A Novel Prototype Neonatal Resuscitator That Controls Tidal Volume and Ventilation Rate: A Comparative Study of Mask Ventilation in a Newborn Manikin

The objective of this randomized controlled manikin trial was to examine tidal volume (V_T) delivery and ventilation rate during mask positive pressure ventilation (PPV) with five different devices, including a volume-controlled prototype Next Step™ device for neonatal resuscitation. We hypothesized that V_T and rate would be closest to target with the Next Step™. Twenty-five Neonatal Resuscitation Program providers provided mask PPV to a newborn manikin (simulated weight 1 kg) in a randomized order with a self-inflating bag (SIB), a disposable T-piece, a non-disposable T-piece, a stand-alone resuscitation system T-piece, and the Next Step™. All T-pieces used a peak inflation pressure of 20 cmH₂O and a positive end-expiratory pressure of 5 cmH₂O. The participants were instructed to deliver a 5 mL/kg V_T (rate 40–60/min) for 1 min with each device and each of three test lungs with increasing compliance of 0.5, 1.0, and 2.0 mL/cmH₂O. V_T and ventilation rate were compared between devices and compliance levels (linear mixed model). All devices, except the Next Step™ delivered a too high V_T, up to sixfold the target at the 2.0-mL/cmH₂O compliance. The Next Step™ V_T was 26% lower than the target in the low compliance. The ventilation rate was within target with the Next Step™ and SIB, and slightly lower with the T-pieces. In conclusion, routinely used newborn resuscitators over delivered V_T, whereas the Next Step™ under delivered in the low compliant test lung. The SIB had higher V_T and rate than the T-pieces. More research is needed on volume-controlled delivery room ventilation.

Introduction

Inappropriate tidal volume (V_T) delivery during positive pressure ventilation (PPV) has been associated with an increased risk of both brain and lung injury in preterm infants (1, 2). Thus, modern mechanical ventilators use flow sensors to deliver volume-targeted ventilation. Perinatal transition involves rapidly changing lung mechanics (3), and despite the increasing focus on volume-targeted ventilation, commonly used neonatal resuscitation devices (4, 5) [e.g., self-inflating bags (SIBs) or T-piece devices] are pressure limited. If PPV is required, the inflation pressure and inflation time should be adjusted to deliver an adequate V_T as the lungs are gradually aerating and clearing lung liquid (6–8). However, complicating the task of providing PPV in the delivery room (DR) is the fact that the “appropriate” V_T varies in the first minutes after birth (9, 10). We have previously demonstrated that T-piece and SIB V_T were above the target in an intubated model with high airway compliance when a set peak inflation pressure (PIP) was used during PPV (11). However, mask ventilation differs from ventilation using an endotracheal tube (ETT) in many aspects. Distension of the facemask and upper airways during PPV might contribute to a difference in V_T needed to achieve adequate lung inflation during mask PPV compared to ETT ventilation (12). Mask leak and airway obstruction are other significant complicating factors during mask PPV (13, 14).

Thus, the primary aim of this study was to compare the same commonly used neonatal resuscitators that we used in our previous ETT study (11), to assess V_T delivery and ventilation rate during mask PPV with changing airway compliance in a newborn manikin. Also, similar to our previous study, we added a prototype ventilation device designed to deliver a predetermined V_T and ventilation rate irrespective of airway compliance (Next Step™, KM Medical, Auckland, New Zealand) for comparison. We hypothesized that the Next Step™ V_T delivery and ventilation rate would be most consistent compared to all other devices.

Materials and Methods

Environment and Subjects

The study was carried out in July 2015 at The Royal Alexandra Hospital, Edmonton, a tertiary perinatal center with an annual delivery rate of >7,000, and admitting approximately 1,500 infants to the Neonatal Intensive Care Unit (NICU) each year. The Northern Alberta Neonatal Program Research Committee and Health Ethics Research Board, University of Alberta approved the study. Registered Neonatal Resuscitation Program (NRP) health-care professionals including neonatologists, neonatal fellows, neonatal nurse practitioners, respiratory therapists, and neonatal nurses were included after written informed consent. The most commonly used neonatal resuscitator used by the participants was either T-piece device tested in the present study. The SIB was infrequently used.

Randomization

This was a randomized, prospective, experimental study. All participants performed PPV with five ventilation devices in a randomized order. The primary investigator (Anne Lee Solevåg) conducted the randomization using an online tool¹ and a code list. As this was a manikin study, the trial was not registered in the clinical trials database.²

Sample Size Estimation

Our primary outcome was V_T delivery. A sample size of 25 NRP providers would be sufficient to detect a 40% more accurate V_T delivery with the Next Step™ device compared to a pressure-limited device, which we considered clinically important with 80% power and a two-tailed alpha error of 0.05.

Manikin

We used a NeoNatalie manikin (Laerdal Medical, Stavanger, Norway) modified with rubber tubing connecting the manikin’s pharynx with test lungs with three different compliance levels (Figure 1). The manikin did not exhibit chest rise, and its exterior resembled a term infant.

FIGURE 1

Figure 1. Test lung components. The upper airway of the manikin was connected via rubber tubing to three external test lungs. The cylinder test lungs were designed to exhibit a compliance of 0.5 mL/cmH₂O (C0.5), 1.0 mL/cmH₂O (C1), and 2.0 mL/cmH₂O (C2), respectively. ADC, analog-to-digital converter.

Test Lungs and Integrated Flow Sensor

Details about the three aluminum cylinder test lungs with integrated flow sensor are presented in Ref. (11). In agreement with clinical data (15), the test lungs had compliances of (i) 0.5 mL/cmH₂O, (ii) 1.0 mL/cmH₂O, and (iii) 2.0 mL/cmH₂O, respectively. The test lungs were kept in a case and connected to the upper airways of the manikin by rubber tubing (Figure 1). The test lung settings were masked to participants during the study. Prior to each study day, the test lungs were checked for accuracy using a 10-mL glass syringe.

Ventilation Devices and Mask

Prior to the experiments, the participants had time to familiarize themselves with each ventilation device. (1) A SIB with a 35-cmH₂O pop-off valve (Laerdal Medical, Stavanger, Norway), no PEEP valve, or manometer attached; (2) Neo-Tee disposable T-piece (Mercury Medical, Clearwater, FL, USA) with PIP 20 cmH₂O and PEEP 5 cmH₂O; (3) Neopuff™ Infant T-piece (Fisher & Paykel, Auckland, New Zealand) with PIP 20 cmH₂O and PEEP 5 cmH₂O; (4) Giraffe Stand-alone Infant Resuscitation System T-piece (GE Healthcare, Buckinghamshire, UK) with PIP 20 cmH₂O and PEEP 5 cmH₂O; and (5) The Next Step™ (KM Medical, Auckland, New Zealand) with a 5-cmH₂O PEEP valve. The Next Step™ neonatal resuscitator (Figure 2) controls V_T and ventilation rate and monitors airway pressure. The 800g prototype is operated via a tablet displaying PIP, PEEP, and V_T in real time. According to the manufacturer, the device delivers V_T with an accuracy of 0.1–0.3 mL and has an internal battery with a life of ≥4 h in the standard configuration. For all devices, a Neonatal Clear Anatomical Face Mask (Mercury Medical) was used.

FIGURE 2

Figure 2. Current prototype of the KM Medical Next Step™.

Experimental Protocol

Each participant used all five ventilation devices in a randomized order, delivering PPV with each device for 3 × 1 min (1 min for each compliance level) targeting a rate of 40–60/min. The Next Step™ ventilation rate was set at 50/min. The 1-min ventilation sessions were separated by a minute during which compliance and/or ventilation device was changed and the participants were allowed to recover. The compliance levels were used in a predetermined order (0.5, 1.0, and 2.0 mL/cmH₂O) for each device to simulate the changing airway compliance after birth (15, 16). Although participants knew that the compliance would change after each minute of PPV, they were unaware of the starting compliance level. Since small preterm infants are most prone to ventilation-induced lung injury (17), the experimental setup was that of a 1-kg infant and a target V_T of 5 mL/kg (18). During the experiments, participants were reminded to provide a targeted V_T of 5 mL/kg, but were blinded to respiratory data recordings, except when using the Next Step™, which has a screen displaying airway pressures and V_T in real time. After completion of all experiments, each participant was asked to complete a brief questionnaire. Questions included (i) “difficulty of providing PPV with each device”; (ii) “comfort with each device to provide PPV”; and (iii) preferred ventilation device; as well as profession, years of experience, and gender. Questions (i) and (ii) were rated using a Likert scale [1 (very difficult/uncomfortable) to 5 (very easy/comfortable)].

Data Processing and Statistical Analyses

Demographical data and device preferences are presented as numbers and percentages or median with range or interquartile range (IQR). The primary outcome parameters of the study (V_T and ventilation rate with each device and compliance level) were tested for normality and presented as mean with 95% confidence interval. The outcome parameters were nested within participants, and a cluster effect was likely to be present in the data. As observations within the same participant tended to be correlated, differences between ventilation devices were estimated using a linear mixed model treating device and compliance as fixed effects and participants as random effects. Adjustments were made for multiple comparisons. Categorical data were compared between devices with the Pearson’s Chi-squared test.

Statistical analyses were performed in IBM SPSS Statistics 22 (IBM Corporation, Armonk, NY, USA). A p-value <0.05 was considered significant.

Results

Twenty-five NRP providers were recruited for the study [neonatologists (n = 4), neonatal fellows (n = 5), neonatal nurse practitioners (n = 4), respiratory therapists (n = 8), and neonatal nurses (n = 4)]. Nineteen (76%) of the participants were female. Participants had a median (range) of 6 (1–26) years of neonatal experience, and 10 (0–22) months had passed since their last NRP update.

Tidal Volume Delivery

The Next Step™ delivered the most consistent V_T at all compliance levels, but the V_T was 26% lower than the target at the lowest compliance level (0.5 mL/cmH₂O). The SIB delivered a higher V_T than all other devices at all compliance levels. V_T delivery was similar between T-piece devices in all three compliance levels. In summary, all ventilation devices except the Next Step™ delivered V_T, which was twofold to sixfold higher than our target V_T with increasing compliance levels (Table 1).

TABLE 1

Table 1. Tidal volumes during 1 min of positive pressure ventilation with different ventilation devices and compliance levels.

Ventilation Rate

Insignificant variation in the Next Step™ ventilation rate was observed (Table 2). By the design of the study, the ventilation rate with the Next Step™ was significantly different from that of the other devices i.e., set at 50 inflations/min, whereas the rate for the other devices was operator dependent. For the other devices, the ventilation rate was within 40–60/min at all compliance levels only with the SIB. The T-piece ventilation rates were slightly below the target at all compliance levels.

TABLE 2

Table 2. Health-care professionals’ Likert scale rating of how difficult and comfortable [1 (very difficult/uncomfortable) to 5 (very easy/comfortable)] it was to use the different ventilation devices.

Questionnaire results are presented in Table 2. The Next Step™ was rated the easiest and the SIB the most difficult device to use. There was a significant difference in the number of participants preferring each device: 10 (40%) participants preferred the Next Step™, 9 (36%) preferred the Neopuff T-piece, 4 (16%) preferred the Giraffe T-piece, 1 (4%) preferred the Neo-Tee, and 1 (4%) answered “any T-piece device” (p < 0.001). The main difference was between the Neo-Tee and the Next Step™ (p = 0.002), and the SIB and the Next Step™ (p < 0.001).

Discussion

In this randomized, controlled study comparing V_T delivery during mask ventilation at different compliance levels, we found that V_T delivery with widely used neonatal resuscitators was twofold to sixfold higher than our targeted V_T at high airway compliance, whereas volume delivery was lower than targeted with a prototype volume-controlled resuscitator at the lowest compliance.

During mask ventilation, the proportion of the V_T that enters the gas exchanging regions of the lungs may be affected by some of the volume being retained in the oropharynx and upper trachea (12). van Vonderen et al. (12) found that equivalent inflation pressures (25 cmH₂O) resulted in significantly higher V_Ti (11.1 vs. 5.8 mL/kg) and V_Te (8.3 vs. 4.9 mL/kg) during mask ventilation in 10 preterm infants compared to ETT ventilation in the same infants after intubation. We have previously studied ETT ventilation in our manikin model (11), and when we compare the ETT results with mask data from the present study, we also find higher V_T delivery at the low and high compliance setting using a facemask compared to ETT. However, with a compliance of 1 mL/cmH₂O, there is no difference in V_T delivery using a facemask or ETT with either device in our model.

T-Piece Resuscitators

The participants in the current study were not allowed to adjust the PIP on the T-piece devices, which potentially could have yielded even higher V_T than measured. On the other hand, a decrease in PIP with increasing compliance might have resulted in lower V_T delivery. Although compliance changes during lung aeration at birth might be detected during PPV using a flow-inflating bag (19), it remains challenging using either a SIB or T-piece device (20–22). In fact, our results support this claim as we observed increasing V_T with increasing compliance levels (Table 1), despite the fact that the T-pieces are the devices most extensively used by the study participants. This is further supported by Huynh et al. (23), who reported that participants are unable to assess effectiveness of V_T delivery during compliance changes.

T-piece resuscitators have several advantages compared to a SIB, including more consistent V_T delivery (24, 25) and airway pressure (5, 10, 26–28), which was also observed in the current study and our previous ETT study (11).

Self-Inflating Bag

The fact that SIBs are readily available in our NICU but rarely used might partly explain the fact that the participants found the SIB relatively difficult and uncomfortable to use during mask PPV (Table 2). A T-piece requires a gas source resulting in pressurizing the facemask, which could cause higher mask leak and lower V_T compared to the SIB. We did not measure mask leak, but this might have contributed to the higher V_T delivery with the SIB. However, the general lack of experience with the SIB might also have introduced a bias toward a greater deviation from target V_T with this device in our participants.

The Next Step™

The prototype volume-controlled resuscitator used in this experiment utilizes room air and does not require a pressurized gas source. It can deliver supplementary oxygen if connected to a gas tank or oxygen outlet. Similar to our findings in an intubated manikin (11), the Next Step™ provided the most consistent V_T of all devices in the current study using a facemask (Table 1). Participants mentioned that the mask hold with the Next Step™ was unfamiliar, which might have resulted in a higher mask leak and V_T below target in the low compliance setting, especially since the low compliance was the first compliance tested. This suggests that health-care personnel should be familiar with the ventilation device they commonly use. However, despite this, the Next Step™ was ranked number one for preferred device as well as easiest to be used (Table 2). These are very important attributes, as the DR is often a stressful environment, where decisions are made quickly and resuscitators need to have good mask ventilation skills. In addition, the Next Step™ provides visual feedback by displaying delivered airway pressures and V_T during PPV. Although respiratory function monitoring is routinely used in the NICU (29), it is not commonly used in the DR during resuscitation (28, 30). Compared to a respiratory function monitor, the Next Step™ is expected to be cheaper. Also, being smaller and portable, the Next Step™ offers an advantage in units without a designated resuscitation area, i.e., where resuscitation is performed in the delivery suite. With these features, the Next Step™ device is a promising new device for neonatal resuscitation.

Ventilation Rate

Even though the ventilation rate was close to the 40–60/min target with the SIB and T-piece devices, the 95% confidence intervals indicate a variable rate during manual PPV. Preterm infants may have impaired cerebral autoregulation (31), and changes in minute ventilation caused by inconsistent ventilation rate may result in blood CO₂ fluctuations, which again have a theoretical potential for causing damage to the immature brain (32). Achieving more consistent ventilation rates in the DR offers a potential for improved outcomes.

Limitations

Using a manikin to simulate DR resuscitation has its limitations, as it does not resemble real-life resuscitation. However, new ventilation devices have to undergo extensive bench-top testing prior to introduction into the DR. During real-life resuscitation, clinicians can use clinical cues (e.g., changes in heart rate) to adjust their ventilation efforts (33), which manikins do not have. Furthermore, the manikin resembled a term infant rather than a 1-kg preterm infant and chest rise was not visible as the manikin was connected to the external test lungs, which might have caused higher V_T delivery. We tried to reduce this bias by continuously reminding the participants that they should aim for a 5-mL V_T/kg.

In conclusion, the Next Step™, a volume-controlled device, had the most consistent V_T delivery and ventilation rate compared to all other devices, and the SIB over delivered to the greatest extent. Participants were unable to recognize compliance changes. The Next Step™, a volume-targeted neonatal resuscitation device has the potential to provide a lung-protective strategy from birth both using a facemask and ETT, but requires further investigation.

Author Contributions

AS conceptualized and designed the study; performed the data collection, data analysis, and data interpretation; and wrote the manuscript. EH, P-YC, and GS conceptualized and designed the study, was involved in the data analysis and data interpretation, critically reviewed the manuscript, and approved the final manuscript as submitted. SO and KB conceptualized and designed the study, was involved in the data interpretation, critically reviewed the manuscript, and approved the final manuscript as submitted.

Conflict of Interest Statement

KM Medical provided The Next Step™ Neonatal Resuscitator for the study. The company was involved in the design of the study, but not in the data acquisition, data analysis, interpretation of the results, or writing of the manuscript. The test lung was designed and manufactured by Auckland University of Technology under the supervision of EH. Ms. SO and AS, KB, P-YC, and GS have no potential conflicts of interest relevant to this article to disclose. EH was in 2008 involved as a University of Auckland Senior Lecturer in the prototype design of the Next Step™ under a research and consultancy agreement between Auckland UniServices Limited and KM Medical (Auckland, New Zealand). Ms. SO and AS, KB, P-YC, and GS have no financial relationships relevant to this article to disclose.

Acknowledgments

The authors would like to thank the neonatal health-care professionals who have contributed to this study.

Funding

The authors would like to thank the public for their donations to our funding agencies. AS is supported by the Canadian Institutes of Health Research [operating grant (MOP-CIA-299111) to P-YC and travel award to AS] and the South-Eastern Norway Regional Health Authority. GS is supported by a Heart and Stroke Foundation/University of Alberta Professorship for Neonatal Resuscitation and by a Heart and Stroke Foundation of Canada Research Scholarship. Ms. SO and EH, KB, and P-YC did not receive funding for work related to this article. Sponsors of the study had no role in study design, data collection, data analysis, data interpretation, or writing of the report.

Footnotes

1. ^http://www.randomizer.org.
2. ^http://clinicaltrials.gov.

References

1. Keszler M. Volume-targeted ventilation. J Perinatol (2005) 25(Suppl 2):S19–22. doi: 10.1038/sj.jp.7211313

PubMed Abstract | CrossRef Full Text | Google Scholar

2. Barton SK, Tolcos M, Miller SL, Roehr CC, Schmölzer GM, Davis PG, et al. Unraveling the links between the initiation of ventilation and brain injury in preterm infants. Front Pediatr (2015) 3:97. doi:10.3389/fped.2015.00097

PubMed Abstract | CrossRef Full Text | Google Scholar

3. Hooper SB, Siew ML, Kitchen MJ, te Pas AB. Establishing functional residual capacity in the non-breathing infant. Semin Fetal Neonatal Med (2013) 18:336–43. doi:10.1016/j.siny.2013.08.011

PubMed Abstract | CrossRef Full Text | Google Scholar

4. Schmölzer GM, Olischar M, Raith W, Resch B, Reiterer F, Müller W. Delivery room resuscitation. Monatsschr Kinderheilkd (2010) 158:471–6. doi:10.1007/s00112-010-2171-y

CrossRef Full Text | Google Scholar

5. Leone TA, Rich W, Finer NN. A survey of delivery room resuscitation practices in the United States. Pediatrics (2006) 117:e164–75. doi:10.1542/peds.2005-0936

PubMed Abstract | CrossRef Full Text | Google Scholar

6. Davis JM, Veness-Meehan K, Notter RH, Bhutani VK, Kendig JW, Shapiro DL. Changes in pulmonary mechanics after the administration of surfactant to infants with respiratory distress syndrome. N Engl J Med (1988) 319:476–9. doi:10.1056/NEJM198808253190804

PubMed Abstract | CrossRef Full Text | Google Scholar

7. Avery ME, Fletcher BD, Williams RG. The lung and its disorders in the newborn infant. Major Probl Clin Pediatr (1981) 1(4th Edition):1–367.

Google Scholar

8. Kattwinkel J, Perlman JM, Aziz K, Colby C, Fairchild K, Gallagher J, et al. Part 15: neonatal resuscitation: 2010 American Heart Association Guidelines for Cardiopulmonary Resuscitation and Emergency Cardiovascular Care. Circulation (2010) 122:S909–19. doi:10.1161/CIRCULATIONAHA.110.971119

CrossRef Full Text | Google Scholar

9. van Vonderen JJ, Kamlin CO, Dawson JA, Walther FJ, Davis PG, te Pas AB. Mask versus nasal tube for stabilization of preterm infants at birth: respiratory function measurements. J Pediatr (2015) 167:81–5.e1. doi:10.1016/j.jpeds.2015.04.003

PubMed Abstract | CrossRef Full Text | Google Scholar

10. Schmölzer GM, Kamlin OC, O’Donnell CP, Dawson JA, Morley CJ, Davis PG. Assessment of tidal volume and gas leak during mask ventilation of preterm infants in the delivery room. Arch Dis Child Fetal Neonatal Ed (2010) 95:F393–7. doi:10.1136/adc.2009.174003

PubMed Abstract | CrossRef Full Text | Google Scholar

11. Solevåg AL, Haemmerle E, van Os S, Bach KP, Cheung PY, SchmÖlzer GM. Comparison of positive pressure ventilation devices in a newborn manikin. J Matern Fetal Neonatal Med (2016):1–5. doi:10.1080/14767058.2016.1180360

PubMed Abstract | CrossRef Full Text | Google Scholar

12. van Vonderen JJ, Hooper SB, Krabbe VB, Siew ML, te Pas AB. Monitoring tidal volumes in preterm infants at birth: mask versus endotracheal ventilation. Arch Dis Child Fetal Neonatal Ed (2015) 100:F43–6. doi:10.1136/archdischild-2014-306614

PubMed Abstract | CrossRef Full Text | Google Scholar

13. Finer NN, Rich W, Wang C, Leone T. Aiway obstruction during mask ventilation of very low birth weight infants during neonatal resuscitation. Pediatrics (2009) 123:865–9. doi:10.1542/peds.2008-0560

CrossRef Full Text | Google Scholar

14. Schmölzer GM, Dawson JA, Kamlin CO, O’Donnell CP, Morley CJ, Davis PG. Airway obstruction and gas leak during mask ventilation of preterm infants in the delivery room. Arch Dis Child Fetal Neonatal Ed (2011) 96:F254–7. doi:10.1136/adc.2010.191171

PubMed Abstract | CrossRef Full Text | Google Scholar

15. Hull D. Lung expansion and ventilation during resuscitation of asphyxiated newborn infants. J Pediatr (1969) 75:47–58. doi:10.1016/S0022-3476(69)80100-9

CrossRef Full Text | Google Scholar

16. Vilstrup CT, Bjorklund LJ, Werner O, Larsson A. Lung volumes and pressure-volume relations of the respiratory system in small ventilated neonates with severe respiratory distress syndrome. Pediatr Res (1996) 39:127–33. doi:10.1203/00006450-199601000-00019

PubMed Abstract | CrossRef Full Text | Google Scholar

17. Jobe AH, Hillman N, Polglase G, Kramer BW, Kallapur S, Pillow J. Injury and inflammation from resuscitation of the preterm infant. Neonatology (2008) 94:190–6. doi:10.1159/000143721

PubMed Abstract | CrossRef Full Text | Google Scholar

18. Mian QN, Pichler G, Binder C, O’Reilly M, Aziz K, Urlesberger B, et al. Tidal volumes in spontaneously breathing preterm infants supported with continuous positive airway pressure. J Pediatr (2014) 165:702–6.e1. doi:10.1016/j.jpeds.2014.06.047

PubMed Abstract | CrossRef Full Text | Google Scholar

19. Bowman TA, Paget-Brown A, Carroll J, Gurka MJ, Kattwinkel J. Sensing and responding to compliance changes during manual ventilation using a lung model: can we teach healthcare providers to improve? J Pediatr (2012) 160:372–6.e1. doi:10.1016/j.jpeds.2011.09.028

PubMed Abstract | CrossRef Full Text | Google Scholar

20. Kattwinkel J, Stewart C, Walsh B, Gurka M, Paget-Brown A. Responding to compliance changes in a lung model during manual ventilation: perhaps volume, rather than pressure, should be displayed. Pediatrics (2009) 123:e465–70. doi:10.1542/peds.2008-2012

PubMed Abstract | CrossRef Full Text | Google Scholar

21. Boldingh AM, Solevåg AL, Benth JS, Klingenberg C, Nakstad B. Newborn manikin study shows that physicians often fail to detect correct lung compliance when using a self-inflating bag. Acta Paediatr (2016) 105:172–7. doi:10.1111/apa.13114

PubMed Abstract | CrossRef Full Text | Google Scholar

22. Klingenberg C, Dawson JA, Gerber A, Kamlin CO, Davis PG, Morley CJ. Sustained inflations: comparing three neonatal resuscitation devices. Neonatology (2011) 100:78–84. doi:10.1159/000322983

PubMed Abstract | CrossRef Full Text | Google Scholar

23. Huynh T, Hemway RJ, Perlman J. Assessment of effective mask ventilation is compromised during synchronized chest compressions. Arch Dis Child Fetal Neonatal Ed (2015) 100:F39–42. doi:10.1136/archdischild-2014-306309

CrossRef Full Text | Google Scholar

24. Roehr CC, Kelm M, Fischer HS, Buhrer C, Schmalisch G, Proquitte H. Manual ventilation devices in neonatal resuscitation: tidal volume and positive pressure-provision. Resuscitation (2010) 81:202–5. doi:10.1016/j.resuscitation.2009.10.008

PubMed Abstract | CrossRef Full Text | Google Scholar

25. Roehr CC, Kelm M, Proquitte H, Schmalisch G. Equipment and operator training denote manual ventilation performance in neonatal resuscitation. Am J Perinatol (2010) 27:753–8. doi:10.1055/s-0030-1254236

PubMed Abstract | CrossRef Full Text | Google Scholar

26. Bennett S, Finer NN, Rich W, Vaucher Y. A comparison of three neonatal resuscitation devices. Resuscitation (2005) 67:113–8. doi:10.1016/j.resuscitation.2005.02.016

CrossRef Full Text | Google Scholar

27. Finer NN, Rich W, Craft A, Henderson C. Comparison of methods of bag and mask ventilation for neonatal resuscitation. Resuscitation (2001) 49:299–305. doi:10.1016/S0300-9572(01)00311-2

PubMed Abstract | CrossRef Full Text | Google Scholar

28. Schmölzer GM, Morley CJ, Wong C, Dawson JA, Kamlin CO, Donath SM, et al. Respiratory function monitor guidance of mask ventilation in the delivery room: a feasibility study. J Pediatr (2012) 160:377–81.e2. doi:10.1016/j.jpeds.2011.09.017

PubMed Abstract | CrossRef Full Text | Google Scholar

29. Morley CJ, Keszler M. Ventilators do not breathe. Arch Dis Child Fetal Neonatal Ed (2012) 97:F392–4. doi:10.1136/fetalneonatal-2012-302137

CrossRef Full Text | Google Scholar

30. Schilleman K, Siew ML, Lopriore E, Morley CJ, Walther FJ, te Pas AB. Auditing resuscitation of preterm infants at birth by recording video and physiological parameters. Resuscitation (2012) 83:1135–9. doi:10.1016/j.resuscitation.2012.01.036

PubMed Abstract | CrossRef Full Text | Google Scholar

31. Greisen G. Autoregulation of cerebral blood flow in newborn babies. Early Hum Dev (2005) 81:423–8. doi:10.1016/j.earlhumdev.2005.03.005

CrossRef Full Text | Google Scholar

32. Fenton AC, Woods KL, Evans DH, Levene MI. Cerebrovascular carbon dioxide reactivity and failure of autoregulation in preterm infants. Arch Dis Child (1992) 67:835–9. doi:10.1136/adc.67.7_Spec_No.835

PubMed Abstract | CrossRef Full Text | Google Scholar

33. Poulton DA, Schmölzer GM, Morley CJ, Davis PG. Assessment of chest rise during mask ventilation of preterm infants in the delivery room. Resuscitation (2011) 82:175–9. doi:10.1016/j.resuscitation.2010.10.012

PubMed Abstract | CrossRef Full Text | Google Scholar