ASIC3 plays a protective role in delayed-onset muscle soreness (DOMS) through muscle acid sensation during exercise

Immediate exercise-induced pain (IEIP) and DOMS are two types of exercise-induced muscle pain and can act as barriers to exercise. The burning sensation of IEIP occurs during and immediately after intensive exercise, whereas the soreness of DOMS occurs later. Acid-sensing ion channels (ASICs) within muscle afferents are activated by H⁺ and other chemicals and have been shown to play a role in various chronic muscle pain conditions. Here, we further defined the role of ASICs in IEIP, and also tested if ASIC3 is required for DOMS. After undergoing exhaustive treadmill exercise, exercise-induced muscle pain was assessed in wild-type (WT) and ASIC3^−/− mice at baseline via muscle withdrawal threshold (MWT), immediately, and 24 h after exercise. Locomotor movement, grip strength, and repeat exercise performance were tested at baseline and 24 h after exercise to evaluate DOMS. We found that ASIC3^−/− had similar baseline muscle pain, locomotor activity, grip strength, and exercise performance as WT mice. WT showed diminished MWT immediately after exercise indicating they developed IEIP, but ASIC3^−/− mice did not. At 24 h after baseline exercise, both ASIC3^−/− and WT had similarly lower MWT and grip strength, however, ASIC3^−/− displayed significantly lower locomotor activity and repeat exercise performance at 24 h time points compared to WT. In addition, ASIC3^−/− mice had higher muscle injury as measured by serum lactate dehydrogenase and creatine kinase levels at 24 h after exercise. These results show that ASIC3 is required for IEIP, but not DOMS, and in fact might play a protective role to prevent muscle injury associated with strenuous exercise.

1. Introduction

Exercise is increasingly considered as a non-pharmacological, low cost and readily available first-line therapy for many chronic disease conditions (1–4), as well as to prevent the development of chronic pain (5). However, despite the many benefits of regular physical activity, exercise can induce pain and fatigue (6). These negative responses to exercise can be barriers to participating in regular physical activity, especially in individuals already suffering from chronic pain and fatigue conditions.

Immediate exercise-induced pain (IEIP) and delayed onset muscle soreness (DOMS) are two types of muscle pain induced by exercise. IEIP is generally described as a burning sensation usually experienced during and immediately after intense exercise and is believed to be caused by the accumulation of metabolites (e.g., H⁺, lactate, and ATP) within the exercising muscle. DOMS, on the other hand, is an aching muscle pain and discomfort that peaks between 24 and 48 h after exercise and is caused by excessive and unaccustomed muscle contractions. Besides muscle soreness, DOMS is also characterized by stiffness, weakness, tenderness, and diminished movement (7–10).

While the mechanisms underlying DOMS are incompletely understood, the most accepted purported causes involve reversible microscopic damage and associated inflammation of muscle fibers (7, 9, 11–13). These micro-injuries lead to the recruitment of neutrophils and macrophages that release several inflammatory and growth factors. The accumulation of metabolites during and immediately after exercise, and the increase in inflammatory mediators and neurotrophic factors occurring 1–2 days later, are sensed by receptors on muscle sensory (afferent) neurons, which are in turn activated and signal the sensations of IEIP and DOMS. A likely candidate sensor of these chemical perturbations within muscle afferents are acid-sensing ion channels (ASICs) (12, 14–17).

ASICs are H⁺-gated ion channels of the DEG/ENaC family principally expressed in central and peripheral sensory neurons (18, 19). They include four genes (ASIC1, -2, -3, and -4) that encode for six subunits (ASIC1 and -2 both have alternative splice transcripts: ASIC1a, -1b, -2a, and -2b) (20–22). Functional ASIC channels consist of a complex of three subunits; individually expressed subunits form homomultimeric channels, whereas coexpression of two or more subunits generates heteromultimeric channels (23). They are highly expressed in skeletal muscle afferents (18, 24), where they play several important functions. First, ASICs are poised to sense chemical changes with skeletal muscle—they are activated in the range of extracellular pH (7.0–6.8) that occurs during muscle ischemia and exercise, and are potentiated by other chemicals during muscle ischemia, hypoxia, and/or exercise (e.g., lactate, ATP, arachidonic acid, and nitric oxide) (14–17, 25, 26). We studied isolated labeled muscle afferents from mice and found that ∼70% displayed ASIC-like currents, and the subunit composition was a heteromeric channel consisting primarily of ASIC1a, -2a, and -3 subunits (27). Second, ASICs are required for the development of chronic muscle pain. Either genetic deletion, RNAi knockdown in muscle afferents, or pharmacological inhibition of ASICs attenuates hyperalgesia in rodent models of chronic muscle pain (28–31). Third, ASICs play an important role in muscle afferents during intense exercise. Type III (thinly myelinated) and type IV (unmyelinated) muscle afferents are activated by both mechanical and/or metabolic (chemical) stimuli during exercise, which triggers reflexive increases in BP, HR, and ventilation via sympathetic nerve activity (termed the “exercise pressor reflex”) (29, 32, 33). In a series of studies, pharmacological or genetic inhibition of ASICs have been shown to attenuate the metabolic component, but not the mechanical component, of the exercise pressor reflex (34–39). Additionally, we recently showed that ASICs are required for IEIP—genetic deletion of ASIC3 abolished muscle hyperalgesia immediately after exhaustive exercise (40). Additionally, exercise training downregulated the expression of ASICs in muscle afferents, and was associated with lower IEIP and improved exercise performance after training (40).

There is also evidence that ASICs might play a role in a rat model of DOMS. Fuji et al. found that intraperitoneal or intramuscular injection of an ASICs antagonist (amiloride) prevented muscle hyperalgesia induced by electrically stimulated eccentric muscle contraction (41). More recently, this same group found that intramuscular injection of APETx2, a selective blocker of ASIC3, similarly prevented muscle hypersensitivity and suppressed the hyperexcitability of isolated muscle afferent fibers after eccentric contraction (42).

While acknowledging the above data supporting a role for ASICs in DOMS, we also reasoned that ASICs could have an opposite effect and might actually serve a protective role to attenuate DOMS. While exercise performance can be limited by the sensations of muscle pain (43, 44), acute pain can also be protective against harm and muscle injury (45–47). We hypothesized that IEIP could serve as an alarm to prevent over-exercising and given that ASIC3^−/− mice do not sense IEIP they might experience more muscle fatigue and potentially more injury during exhaustive exercise. Additionally, loss of normal increases in blood pressure, heart rate, and respiration via the exercise pressor reflex in ASIC3^−/− mice might induce more physiological stress on the muscle during exercise. Thus, the current study was conducted to (1) further our understanding of the role of ASICs on muscle pain immediately after exhaustive exercise, and (2) to investigate the role of ASICs in DOMS using a genetic knockout model of ASIC3. We chose to study ASIC3 because it is not expressed in the central nervous system in mice (only expressed in peripheral sensory neurons), and it plays a large role in the biophysical properties of ASIC currents in mouse muscle afferents (27).

2. Methods

2.1. Ethical approval

All experimental procedures and protocols were approved by the Institutional Animal Care and Use Committee of the University of Iowa (Protocol no. 1990903) and conformed to the national guidelines set by the Association for Assessment and Accreditation of Laboratory Animal Care. All experiments were carried out according to the guidelines described by Grundy (48), and we have taken all steps to minimize the pain and suffering of the animals.

2.2. Animals

Male and female 11–12-week-old C57BL/6J (Jackson Laboratory, Sacramento, CA, USA) or ASIC3^−/− mice were acclimated to the new environment and room for 1 week prior to the beginning of treadmill acclimation. The generation of ASIC3^−/− mice has been previously reported (49). These mice were subsequently backcrossed for 10 generations onto a C57BL/6J background to generate a congenic line. The animals were housed in a temperature-controlled room (22°C) with a 12-hour light/dark cycle and had free access to standard mouse food (pellet) and water ad libitum. Mice were euthanized with CO₂ per AVMA guidelines. All tests were performed by the same experimenter who was blinded to genotype and drug injection in all experiments. All experiments were performed in both male and female mice. We found no differences between sexes and so they were combined in all experiments.

2.3. Exhaustive exercise protocol

Mice were acclimated to a six-lane motor-driven treadmill (Columbus Instruments, Columbus, OH) for 3 days with gradually increasing velocity and incline for 30 min/day. Shock grids at the rear of the treadmill were set at 1 mA and 1 Hz to encourage continued ambulation on the treadmill during acclimation. Exhaustive exercise testing was performed to measure time to exhaustion as previously described (40). Briefly, mice were placed on a non-moving treadmill (20° incline throughout) with a shock bar on for 10 min, followed by a 10-min warm-up at a velocity of 6 m/min. At the beginning of exhaustive exercise testing, we turned off the shock bar and instead used gentle prodding with a tongue depressor to encourage exercise as needed. We found this technique to be less stressful for the mice, and exhaustive exercise capacity was similar compared to using the shock bars (50). After the warm-up, the velocity was increased to 8 m/min, and then increased by 2 m/min every 3 min (velocity accelerations were 2 m/min²) until the mice became exhausted. Exhaustion was defined as the point at which mice stayed at the end of treadmill and did not respond to 10 consecutive nudges with a tongue depressor in 10 s.

2.4. Open field test

For the assessment of locomotor activity, mice were subjected to the open field test before, immediately and 24 h following exercise. Horizontal movements were quantified as the number of photobeam breaks over 30 min in a lighted chamber (40.6 cm wide × 40.6 cm deep × 36.8 cm high, 1,500 lux, 16 × 16 photobeams to record horizontal movements, with 2.54 cm photobeam spacing) (San Diego Instruments).

2.5. Muscle withdrawal threshold (MWT)

MWT was measured by applying a force-sensitive tweezer to the belly of the gastrocnemius muscle as previously described (51), whereby lower thresholds indicate greater sensitivity to mechanical stimuli. Briefly, mice were acclimated to the testing paradigm in two 5-min sessions for 3 days. Then, mice were gently restrained in a gardener's glove, the hindlimb held in extension, and the muscle squeezed with progressive force with the force-sensitive tweezers until the mouse withdrew its hindlimb. Three trials per hindlimb were averaged. In this study, we selected the gastrocnemius muscle because it is the main muscle during running on the treadmill in mice and the fast twitch muscles are more susceptible to damage after exercise (12, 52).

2.6. Grip strength test

Limb grip strength was measured using a grip strength meter (Bioseb, Model: Bio-GT3, France). Mice were acclimated to the apparatus by being placed on the grids twice per day for 5 s in 1 min intervals for 3 consecutive days. Grip strength was then measured by placing mice on the grid and then gently pulling their tail (at a constant speed within 3 s) to measure maximal grip force (calculated by averaging 3 trials conducted at 1 min intervals). The grip test was performed at baseline and 24 h after exercise.

2.7. Lactate measurement

Blood lactate was measured using the tail nick technique to collect 0.5 μl of blood and analyzed via a lactate meter (Nova Biomedical, Waltham, MA) (53).

2.8. Serum CK and LDH assessments

Mice were anesthetized under 2% isoflurane and blood was collected via cardiac puncture. Samples were transferred into a serum collection tube, allowed the blood to clot at room temperature for 20 min, then centrifuged at 3,000 g for 15 min. Frozen serum was shipped to IDEXX BioAnalytics Labs in Sacramento, CA. Serum samples were run using the Beckman Coulter AU680 automated chemistry platform and Beckman Coulter reagents. All chemistry assays are validated for a serum matrix. The analyzer performs photometric and potentiometric assays. Creatine kinase (CK) and lactate dehydrogenase (LDH) are both kinetic enzymatic assays read spectrophotometrically.

2.9. Statistical analysis

GraphPad Prism (8.0) was used to statistically analyze data. Bar graphs represent means ± standard error of the mean (SEM). One-way or two-way ANOVA, or t-tests were used to analyze the results as described in figure legends. Paired or repeated measures testing was done if mice were tested sequentially. If significant differences were observed, then the indicated post hoc tests were performed. Values of P < 0.05 were considered statistically significant. Sample sizes for each experimental protocol were determined based on the effect sizes for changes in the variable from our previous and pilot studies (α = 0.05, β = 0.8; G*Power 3.1 software (54).

2.10. Experimental design

To test for IEIP, separate groups of mice underwent 3 days of treadmill acclimation prior to exhaustive exercise testing (exercise group) or were placed on a non-moving treadmill for the same amount of time (control group). Exercise-induced muscle pain (using MWT), locomotor activity (using open field test), or blood lactate levels were assessed at baseline (a day before exercise), and immediately after exercise (Figure 1A). To test for DOMS (Figure 1B), we acclimated separate groups of mice and performed exhaustive exercise as above, and measured MWT, open field test, grip force, and serum CK and LDH before (baseline) and 24 h after exercise [the peak of DOMS has been observed 12–24 h after exercise in mice (55)]. We also performed the same exhaustive running exercise protocol 24 h after the primary exercise test (re-exercising) (Figure 1B). Each experiment was performed in separate groups and in both male and female WT and ASIC3^−/− mice. We found no differences between male and female mice and so their data is combined. For open field and CK, LDH experiments, separate groups of mice were used at baseline and after exercise due to learning effects (for open field test) and blood collection technique (for CK, LDH assessment), whereas for all other assays mice were tested before and after exercise.

FIGURE 1

Figure 1. Schematic illustrations of experimental designs to test IEIP (A), DOMS (B), and muscle injection protocols (C). Separate groups of mice were used for each experiment. Additionally, separate groups of mice were used pre- and post-exercise for OF and CK, LDH experiments. MWT, muscle withdrawal threshold test; OF, open field test; CK, creatine kinase; LDH, lactate dehydrogenase; APETX2, a selective and reversible ASIC3 inhibitor; PBS, phosphate buffered saline.

In separate studies we injected the ASIC3 inhibitory toxin APETx2 [20 μl; 200 nM in 0.9% sodium chloride (saline)] or phosphate buffered saline [PBS (Sigma, MO, US); 20 μl; 137 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, and 1.8 mM KH₂PO₄, pH 7.4] into the right or left (randomly chosen) gastrocnemius muscle and saline (20 μl) as control into the contralateral muscle 5 min before the exhaustive exercise using a 0.5 ml syringe (31 gauge needle). Muscle withdrawal threshold (MWT) was assessed at baseline (a day before exercise), immediately, and 24 h after exercise (Figure 1C).

3. Results

3.1. ASIC3 is required for immediate exercise-induced muscle pain (IEIP)

We previously found that the genetic deletion of ASIC3 abolished muscle hyperalgesia immediately after exhaustive exercise (40). As a measure of muscle pain, we recorded MWT, whereby force-sensitive tweezers were applied to the gastrocnemius muscle until the limb was withdrawn (a lower MWT indicated increased pain or hyperalgesia) before and immediately after exhaustive exercise in ASIC3^−/− and WT mice. We found that there was no difference in MWT at baseline between ASIC3^−/− and WT. However, WT mice had a significantly lower MWT immediately after exercise indicating higher IEIP, whereas the ASIC3^−/− mice did not show any changes in MWT immediately after exercise indicating no changes in pain perception immediately after exercise (Figure 2A). To test if ASIC3 is required specifically within muscle afferents for IEIP, we injected a specific ASIC3 inhibitory peptide APETx2 (20 μl, 200 nM) into the right or left gastrocnemius muscle (randomly assigned), and a similar volume of saline (control) was injected into the contralateral muscle 5 min before the exercise test. Similar to the genetic deletion of ASIC3, the limb that was injected with saline developed lower MWT immediately after exercise, whereas the decrease in post-exercise MWT was abrogated in the limb that received APETx2 (Figure 2B).

FIGURE 2

Figure 2. Effect of genetic deletion and pharmacological inhibition of ASIC3 on immediate exercise-induced pain (IEIP). (A) Muscle withdrawal threshold (MWT) was measured before (baseline) and immediately after exhaustive exercise in male and female wild-type (WT) and ASIC3^−/− mice. Two-way repeated measures ANOVA showed a significant time effect [F_(1,23) = 35.27, P < 0.0001, n ≥ 12]. Sidak's multiple comparison test found no difference in baseline MWT between groups before exhaustive exercise. On the other hand, there was a significant decrease in MWT immediately after exercise compared with baseline for WT (****P < 0.0001) but not ASIC3^−/− mice. Reproduced and modified from Khataei et al. 2020 (40). (B) Twenty microliter APETX2 (200 nM) was injected into the right or left (randomly chosen) gastrocnemius muscle and saline (20 μl) as control into the contralateral muscle 5 min before the exhaustive exercise. MWT was assessed at baseline and immediately after exhaustive exercise. Two-way repeated measure ANOVA showed a significant time effect [F_(1,16) = 8.25, P < 0.05, n = 9]. Sidak's multiple comparison test discovered MWT was diminished immediately after exercise with saline (P < 0.05) but not with APETX2. (C) Twenty microliter saline or Phosphate-Buffered Saline (PBS) was injected into contralateral gastrocnemius muscles 5 min before the exercise. MWT was assessed at baseline and immediately after exhaustive exercise. Two-way repeated measure ANOVA showed a significant group-time effect [F_(1,18) = 7.69, P < 0.05, n = 10]. Sidak's multiple comparison test discovered MWT was diminished immediately after exercise with saline (P < 0.05) but not with PBS. (D) Blood lactate was measured before and immediately after exhaustion. Two-way repeated measure ANOVA found a significant time effect [F_(1,18) = 144.9, P < 0.0001, n = 10]. Sidak's multiple comparison test showed a significant increase in blood lactate for both WT and ASIC3^−/− mice following exhaustive exercise (P < 0.0001).

Next, we tested if acidosis within the exercising muscle is required for IEIP. We found that PBS injected into the gastrocnemius muscle 5 min before exercise completely abolished the reduction in MWT after exercise, indicating buffering pH within exercising muscle was able to eliminate muscle hyperalgesia immediately after exercise (Figure 2C). We also tested lactate levels immediately after exercise to ascertain if this could account for the differences in pain perception immediately after exercise. Our results showed that both ASIC3^−/− and WT mice had significant increases in serum lactate compared to their baselines, and there was no difference between the two groups (Figure 2D). In summary, these results demonstrate that ASIC3 within muscle afferents is required for muscle hyperalgesia immediately after exhaustive exercise, and that acidosis is a necessary mediator of this increase in pain perception.

3.2. While ASIC3^−/− mice have similar exercise capacity, they have increased fatigue immediately after exercise

Since ASIC3^−/− mice had diminished muscle hyperalgesia immediately after exercise, we hypothesized that they might have higher exercise performance and increased fatigue immediately after exercise. Interestingly, we found ASIC3^−/− mice had a similar time to exhaustion on treadmill testing compared to WT mice (Figure 3A). To test for fatigue, we recorded locomotor activity for 30 min in an open field assay immediately after exhaustive exercise. Figure 3B shows that WT mice had similar locomotor activity after exercise compared to WT mice that were placed on a non-moving treadmill. While there was not a statistical difference between genotypes post-exercise, ASIC3^−/− mice had a significantly lower activity immediately after exercise compared to ASIC3^−/− mice that did not exercise (Figure 3B). Thus, while ASIC3^−/− mice had similar exercise capacity as WT mice, our data suggests they have increased fatigue and delayed recovery after exercise.

FIGURE 3

Figure 3. Effect of genetic deletion of ASIC3 on exercise capacity (A) and locomotor activity immediately after exercise (B). (A) Time to exhaustion (sec) was measured during exhaustive exercise test. Unpaired Student's t-test revealed no difference between groups. (B) Locomotor activity (beam breaks) in open field assay measured for 30 min immediately after exhaustive exercise or mice placed on a non-moving treadmill (control). Two-way ANOVA showed a significant time effect [F_(1,30) = 15.11, P < 0.001, n ≥ 6]. Sidak's multiple comparison test revealed significantly lower total beam breaks for ASIC3^−/− post exercise compared to control ASIC3^−/− mice (P = 0.001) but no difference for WT mice (ns).

3.3. ASIC3^−/− mice experience increased DOMS compared to WT mice

Since ASIC3^−/− mice displayed increased fatigue immediately after exercise, this suggested that perhaps ASICs serve as a signal to prevent overexertion and muscle injury. Thus, we hypothesized that ASIC3^−/− mice might experience altered DOMS compared to WT mice. To test if our exercise protocol induces DOMS in mice, we first tested MWT and grip strength 24 h after exercise. Our results show lower MWT and grip strength 24 h after exercise compared to baseline, indicating the mice developed DOMS, however, the results were not different between WT and ASIC3^−/− mice (Figures 4A,B). These results suggest that while ASIC3 is required for IEIP, it is not required for DOMS.

FIGURE 4

Figure 4. Effect of genetic deletion of ASIC3 on measurements of DOMS. (A) MWT was measured at baseline and 24 h after exhaustive exercise in ASIC3^−/− and WT mice. Two-way repeated measures ANOVA showed a significant time effect [F_(1,30) = 26.27, P < 0.0001, n ≥ 13]. Sidak's multiple comparison test revealed both ASIC3^−/− and WT had a significantly lower MWT 24 h after exercise compared to their baseline (P < 0.01). (B) Grip strength was assessed at baseline and 24 h after exercise. Two-way repeated measure ANOVA showed a significant time effect [F_(1,21) = 55.82, P < 0.0001, n ≥ 11]. Sidak's multiple comparison test revealed both ASIC3^−/− and WT had a significantly lower grip force 24 h after exercise compared to their baseline (P < 0.001 and P < 0.0001, respectively). (C) Locomotor activity was assessed via open field test at baseline and 24 h after exercise. Two-way ANOVA showed a significant group effect [F_(1,60) = 13.31, P < 0.001, n ≥ 13]. Sidak's multiple comparison test revealed significantly lower total beam breaks for ASIC3^−/− compared to WT mice at 24 h after exercise (P < 0.001). (D) Exhaustive exercise performance was measured again 24 h after the preliminary exercise test (re-exercise). Two-way repeated-measures ANOVA showed a significant time effect [F_(1,22) = 12.94, P < 0.01, n = 12]. Sidak's multiple comparison test found ASIC3^−/− mice had significantly lower time to exhaustion compared to the baseline (P < 0.01) and WT mice at 24 h after exercise (P < 0.05).

Since a hallmark of DOMS is a diminishment of activity involving the same muscle groups, we measured locomotor activity and also measured repeated exhaustive exercise capacity at 24 h after the first exercise test. These tests also involve more volitional movement compared to MWT and grip strength tests. We found that ASIC3^−/− mice had significantly lower locomotor activity 24 h after exhaustive exercise compared to WT mice (Figure 4C). Additionally, whereas WT mice had a similar exercise performance on repeat treadmill testing done 24 h after the first test, ASIC3^−/− mice showed significantly reduced maximal exercise time compared to their baseline and compared to WT mice (Figure 4D).

Lastly, we assessed serum CK and LDH as measures of muscle injury 24 h after exhaustive exercise. We found that ASIC3^−/− mice had significantly higher levels of CK and LDH compared to WT mice at 24 h after exercise and compared to baseline (Figures 5A,B). In summary, these results show that ASIC3 is not required for DOMS, and in fact, might play a protective role in diminishing DOMS and/or persistent fatigue after exercise.

FIGURE 5

Figure 5. Serum CK (A) and LDH (B) levels in WT and ASIC3^−/− mice at baseline (control mice) and 24 h after exercise. (A) Two-way ANOVA showed a significant group-time effect [F_(1,36) = 4.73, P < 0.05, n ≥ 9]. Sidak's multiple comparison test revealed a significantly higher CK level for ASIC3^−/− compared to control ASIC3^−/− (P < 0.01) and WT at 24 h after exercise (P < 0.05). (B) Two-way ANOVA showed a significant group-time effect [F_(1,34) = 4.73, P < 0.05, n ≥ 9]. Sidak's multiple comparison test showed a significantly higher LDH level for ASIC3^−/− after exercise compared to control ASIC3^−/− (P < 0.05) and WT at 24 h (P < 0.001).

4. Discussion

During strenuous and unaccustomed exercise metabolites and other chemicals accumulate within muscle. Muscle afferents sense these chemical changes, leading to the sensations of muscle pain and fatigue during and immediately after exercise (IEIP), followed a day or so later by DOMS (56, 57). The characteristics and underlying mechanisms of IEIP and DOMS are different. In this study, we show that ASIC3 is required for IEIP but not DOMS. Additionally, since the sensation of fatigue and pain during exercise might protect muscles from overexercising and injury, we tested whether ASIC3 might also play a protective role to prevent muscle injury and attenuating DOMS. We found that compared to WT mice, ASIC3^−/− mice experienced higher fatigue immediately after exercise. At 24 h after exercise, although both ASIC3^−/− and WT mice showed similar impairment in strength and muscle pain perception compared to their baseline, ASIC3^−/− mice showed diminished spontaneous locomotor activity, lower repeat exercise capacity, and increased muscle injury compared to WT mice. Together, these data show that (1) ASICs are required for IEIP but not DOMS, and (2) ASICs might play a protective role against muscle injury and DOMS after exhaustive exercise.

4.1. Genetic deletion and pharmacological inhibition of ASIC3 diminished immediate exercise-induced muscle pain (IEIP)

Since high-intensity exercise is associated with the accumulation of protons and other metabolites in skeletal muscle, we hypothesized that ASICs would be required for IEIP. We found that strenuous exercise induces IEIP in WT mice, however, pharmacological inhibition (using APETx2) or genetic deletion of ASIC3 completely abolished mechanical hypersensitivity immediately after exercise. Moreover, we found that buffering pH within skeletal muscle also abolished IEIP, although it should be noted that we did not measure the degree to which our PBS injection prevented acidosis within the muscle interstitium.

The properties of ASICs make them ideal sensors of metabolic changes associated with strenuous exercise, and thus mediators of IEIP. During intense exercise, anaerobic metabolism causes a rapid drop in intracellular pH, which in turn leads to acidosis of the muscle interstitium (pH 6.7–7.0 range) (58). ASICs located at the nerve terminals of muscle afferents are robustly activated within this pH range (59). In addition, lactate and ATP levels are significantly increased within muscle interstitium during intense exercise (60, 61), and both these metabolites are potentiators of ASIC currents (15, 17). If fact, this combination of metabolites may serve to synergistically activate ASICs. Light et al. used calcium imaging to identify a population of muscle afferents that were maximally activated by a combination of protons, ATP, and lactate at physiological concentrations, and pharmacological block of ASICs completely inhibited the response to the combination of agonists (25).

Our results in mice are consistent with recent studies in humans. Stavres et al. found that ASICs blocker (10-mg oral dose of amiloride) significantly improved exercise tolerance under blood flow restriction in healthy adults. Their subjects in the amiloride group performed more work and for a longer period at the same relative level of pain (62). Similarly, Campos et al. reported that amiloride infused into the occluded forearm of human subjects reduced perceived discomfort during metaboreflex activation by postexercise muscle ischemia (63). Additionally, we have previously shown that high-intensity interval training abolished IEIP and was associated with diminished mRNA expression of ASICs in muscle afferents in mice—lending insight into how exercise training can reduce pain (40).

4.2. ASIC3 is not required for DOMS

To study DOMS, we tested muscle mechanical hypersensitivity (MWT), strength, spontaneous locomotor activity, re-exercise performance, and chemical measure of muscle injury in ASIC3^−/− and WT mice 24 h after exhaustive exercise. We found that WT and ASIC3^−/− mice had similarly lower MWT and grip strength after exhaustive exercise compared to baseline, indicating both groups developed DOMS. Additionally, in other measures, we found that DOMS might actually be worse in ASIC3^−/− mice compared to WT (discussed in subsequent sections). On the other hand, our mouse ASIC3^−/− models lacks only the ASIC3 subunit, and the other remaining subunits in muscle afferents (ASIC1a and -2a) still form acid-activated channels (27), so perhaps these subunits contribute to DOMS. Nevertheless, we found no evidence that ASIC3 is required for DOMS.

Our results contradict previous findings. In a rat model of DOMS induced by electrically stimulated contraction of the ankle extensor muscle, either amiloride or the specific ASIC3 inhibitor APETx2 prevented the resultant hyperalgesia as measured by muscle probe withdrawal (41, 42). Moreover, this group recorded from isolated muscle afferent fibers and found them to be hyperexcitable in this same rat model of DOMS, and this hypersensitivity was attenuated by APETx2 (42). Besides the obvious species difference between rat and mouse, what else might account for the differences in our results compared to previous studies examining the role of ASICs in DOMS? One major difference is the mechanism by which DOMS was induced. Whereas we used whole-body high-intensity exercise in conscious mice, previous studies deployed electrically stimulating muscle contraction in anesthetized rats. Additionally, they examined the role of ASICs via pharmacological inhibition, while we used a genetic knockout model of mice (ASIC3^−/−). Finally, previous studies employed only mechanical stimulation to assess DOMS. In addition to mechanical MWT, we more broadly assessed DOMS via spontaneous ambulatory and non-spontaneous ambulatory assessments, muscle force production, and biochemical measures of muscle damage.

Perhaps it should not be surprising that ASICs are not required for DOMS. The accumulation of metabolites begins to wash out of muscle soon after the resolution of exercise and pH, lactic acid and ATP levels are normalized to baseline at the time that DOMS is at its peak (58, 64, 65). It is likely that other receptors, such as TRPV1, that are better poised to sense the inflammatory milieu associated with DOMS are more important in the transduction of muscle soreness in the days after exercise (41, 55, 66, 67). Given the difference in our results compared to previous studies, it is also quite likely that the sensory mechanisms of DOMS vary depending on the type and intensity of muscle activity that induce DOMS, and the stimuli that provoke it during the following 1–2 days.

4.3. ASIC3 null mice show increased fatigue immediately after exercise

Since ASIC3^−/− mice have diminished IEIP, and IEIP can be a barrier to higher exercise performance, we hypothesized that ASIC3^−/− mice would have higher exercise capacity and run longer on the treadmill. Furthermore, we expected them to experience higher fatigue after exercise due to a higher workload. While we observed no differences in exercise performance between ASIC3^−/− and WT mice, ASIC3^−/− mice showed greater fatigue (less spontaneous locomotor activity immediately after exhaustive exercise) compared to WT mice.

To begin to understand these seemingly contradictory results, it is necessary to understand the multiple different roles that ASICs play in exercise. Besides triggering sensations of fatigue and pain, activation of group III/IV muscle afferents during exercise increases blood pressure, heart rate, and ventilation (known as the “exercise pressor reflex”) (29). This reflex is important to increase blood flow to exercising muscle and allow maximal exercise performance. Amann et al. has shown that blocking lower extremity group III/IV muscle afferents during human bicycle exercise (by injection of fentanyl intrathecally into the spinal canal at the lumbar level) reduced feedback from exercising muscle and leads to attenuated blood flow to the leg muscles and faster locomotor muscle fatigue (68–72). The Kaufman group has shown that ASICs are required for components of the exercise pressor reflex (34, 37–39). ASIC3 null rats had a significantly attenuated blood pressure response to metabolite infusion (such as lactic acid) (38). Thus, we speculate that in our study ASIC3^−/− mice had (1) diminished IEIP, which would tend to increase exercise performance, and (2) an attenuated exercise pressor reflex, which would tend to lower exercise performance. Consequently, we believe that ASIC3^−/− mice experienced a counterbalance between these two mechanisms which lead to similar exercise performance as WT mice. At the same time, a diminishment of IEIP and the exercise pressor reflex would both be expected to lead to an increase in muscle fatigue in ASIC3^−/− mice during and immediately following exercise. In addition, while we found equal increases in serum lactate levels in ASIC3^−/− compared to WT mice, it is also possible that attenuated muscle blood flow in ASIC3^−/− mice could cause a greater build-up of lactate and other metabolites within exercising muscle, thus contributing to the increase in post-exercise fatigue.

4.4. ASIC3 plays a protective role to reduce DOMS and muscle injury

Since ASICs trigger IEIP, and since ASIC3^−/− mice experienced increased fatigue immediately after exercise, we surmised that sensing metabolites during exhaustive exercise might also serve as an alarm to prevent overexercise and skeletal muscle injury. Thus, we hypothesized that ASIC3^−/− mice would experience more severe DOMS and delayed recovery after exercise. To test this, we measured DOMS by multiple different techniques. We found no difference between MWT and grip strength tests, whereas ASIC3^−/− mice showed less spontaneous locomotor activity (open field) and diminished repeat exhaustive treadmill running compared to WT mice measure at 24 h after the initial treadmill testing.

Why the divergent results? A hallmark of DOMS is that the pain is often related to the movement of the same muscle groups involved in the original exercise, thus resulting in a diminishment of activity involving the same muscle groups. However, both MWT and grip strength tests are non-ambulatory tests—MWT does not involve muscle contraction since force is applied by the experimenter, and during grip strength testing the muscle contraction is isometric. On the other hand, spontaneous locomotor activity (open field) is a measure of volitional fatigue/pain. Moreover, both open field and repeat exhaustive treadmill running are tests involving the use and contraction of the same muscle groups as the initial exhaustive exercise, and both are measures of movement-related pain rather than static pain. For these reasons, we believe that measures of spontaneous locomotion and repeat exercise testing might be more discriminative tests of DOMS compared to MWT and grip strength testing. Additionally, we assessed serum CK and LDH as a biochemical measurement of muscle injury and found that ASIC3^−/− mice had significantly higher levels of CK and LDH compared to WT and their baseline at 24 h after exercise. Thus, these findings suggest that ASIC3 is not only not required for DOMS but may also play a protective function to attenuate DOMS.

In conclusion, these results shed light on the multiple different roles that ASICs play in exercise. We found that although ASIC3 is required for IEIP, it is not required for DOMS and may actually protect against DOMS and muscle injury after high-intensity exercise. These findings have significant implications for health and clinical conditions. Exercise-induced muscle pain can be a barrier to achieving higher exercise performance and can prevent participation in regular physical activity, particularly for people with chronic pain conditions such as fibromyalgia, chronic fatigue, and peripheral artery disease. Understanding the molecular mechanisms of the barriers to exercise can provide a potential target to improve health and fitness.

Data availability statement

The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

Ethics statement

The animal studies were approved by Institutional Animal Care and Use Committee of the University of Iowa. The studies were conducted in accordance with the local legislation and institutional requirements. Written informed consent was obtained from the owners for the participation of their animals in this study.

Author contributions

TK and CB conceived and designed research; TK performed experiments; TK analyzed data; TK and CB interpreted results of experiments; TK prepared figures; TK and CB drafted the manuscript; TK and CB edited and revised the manuscript; All authors contributed to the article and approved the submitted version.

Funding

This study was supported by the Department of Veterans Affairs Merit Award (5I01BX000776).

Acknowledgments

We thank Peter Snyder, Alan Ryan, Anne Harding (University of Iowa) and Markus Amann and Alan Light (University of Utah) for their helpful advice. We also thank IDEXX BioAnalytics Labs for perfect analysis and services throughout our study.

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher's note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

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