This article is a correction to:
DDX19A Promotes Metastasis of Cervical Squamous Cell Carcinoma by Inducing NOX1-Mediated ROS Production
Yanhui Jiang1,2†
Baibin Wang2†Yongliang Li3†
Jiahui Shen1Yutao Wei1Hanjie Li2,4Shangqiu Chen1Hua Yang1Famin Zeng2Changqing Liu1Feng Wang1
Huanhuan He2*
Yong Chen1*‡
Jihong Liu1,5*‡- 1Department of Gynecology, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China
- 2Guangdong Provincial Key Laboratory of Biomedical Imaging and Guangdong Provincial Engineering Research Center of Molecular Imaging, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China
- 3Department of Pathology, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China
- 4Department of Interventional Medicine, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China
- 5Department of Gynecologic Oncology, State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou, China
A Corrigendum on:
DDX19A Promotes Metastasis of Cervical Squamous Cell Carcinoma by Inducing NOX1-Mediated ROS Production
By Jiang Y, Wang B, Li Y, Shen J, Wei Y, Li H, Chen S, Yang H, Zeng F, Liu C, Wang F, He H, Chen Y and Liu J (2021) Front. Oncol. 11:629974. doi: 10.3389/fonc.2021.629974
In the original article, there were errors in Figures 2C, D, 4C and 5D as published. Wrong Western blot pictures were accidentally used in the Figures 2C, D and 4C. The corrected Figure 2 and Figure 4 appear below. There was also an error in the images used in Figure 5D. The corrected Figure 5 appears below.
FIGURE 2
Figure 2 DEAD-box helicase 19A (DDX19A) promotes the metastasis of cervical squamous cell carcinoma (CSCC) cells in vitro and in vivo. (A, B) qRT-PCR and Western blot were employed to evaluate the efficacy of DDX19A mRNA and protein knockdown in SiHa and MS751 (n = 3). (C, D) qRT-PCR and Western blot were employed to evaluate the efficacy of DDX19A mRNA and protein overexpression in SiHa and HCC94 (n = 3). (E, F) Cell migration assay and Matrigel invasion assay were employed to investigate the effect of DDX19A knockdown in SiHa and MS751 cell migration and invasion ability (scale bar: 200μm) (n = 3). (G, H) Cell migration assay and Matrigel invasion assay were employed to investigate the effect of DDX19A overexpression in SiHa and HCC94 cell migration and invasion ability (scale bar: 200μm) (n = 3). (I, J) Arrows showed the representative results of metastatic lung nodules. H&E staining was used to stain metastatic lung nodules (200× and 400× magnification; scale bar: 200μm). Dot plots showed the results of the number of lung metastasis nodules and lung weight (n = 6). Results represent three independent experiments (A–H). The results were shown as means ± SD, **p < 0.01, ***p < 0.001 by two-tailed Student’s t-test.
FIGURE 4
Figure 4 NADPH oxidase 1 (NOX1) promotes metastasis and reactive oxygen species (ROS) production in cervical squamous cell carcinoma (CSCC) cells and may serve as a prognostic marker in CSCC patients. (A, B) qRT-PCR and Western blot were employed to evaluate the knockdown efficacy of NOX1 in SiHa and MS751 (n = 3). (C, D) qRT-PCR and Western blot were employed to evaluate the overexpression efficacy of NOX1 in SiHa and HCC94 (n = 3). (E, F) Cell migration assay and Matrigel invasion assay were employed to investigate the effects of NOX1 knockdown cells (SiHa and MS751) (scale bar: 200μm). (G, H) Cell migration assay and Matrigel invasion assay were employed to investigate the effects of NOX1-overexpressing cells (SiHa and HCC94) (scale bar: 200μm). (I) ROS level was reduced in NOX1 knockdown cells (SiHa and MS751). (J) ROS level was increased in NOX1-overexpressing cells (SiHa and HCC94) (n =3). (K) Representative images of the immunohistochemical (IHC) staining of NOX1 in human CSCC tissues and adjacent non-tumor tissues (scale bar: 200μm). (L) Dot plots to show the IHC score of DDX19A expression using 76 pairs of CSCC tissues and adjacent non-tumor tissues tumor microarray (TMA) tissue sections (p < 0.001). (M) Correlation between lymph node metastasis and DDX19A expression in CSCC patients. Chi-square test was used. (N) Kaplan–Meier analysis was performed for our CSCC patients’ cohort to evaluate the association between DDX19A protein level and 86 CSCC patients’ overall survival. Results represent three independent experiments (A–J). The results were shown as mean ± SD, ***p < 0.001 by two-tailed Student’s t-test.
FIGURE 5
Figure 5 The NADPH oxidase 1 (NOX1)/reactive oxygen species (ROS) axis exerts a pro-metastasis effect downstream of DEAD-box helicase 19A (DDX19A). (A, B) Western blot was employed to evaluate the overexpression efficacy of NOX1 proteins in DDX19A knockdown cell lines (SiHa and MS751) (n = 3). (C, D) Cell migration assay and Matrigel invasion assay were performed to evaluate whether restoring NOX1 expression could increase cellular migration and invasion in DDX19A knockdown cell lines (SiHa and MS751) (n = 3). (E) The 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) fluorescence assay was performed to investigate whether NOX1 overexpression weakens the increase of ROS production induced by DDX19A knockdown in SiHa and MS751. (F) The DCFH-DA fluorescence assay was used to examine the ROS level in DDX19A-overexpressing cell lines (SiHa and HCC94) treated with or without N-acetylcysteine (NAC) (ROS inhibitor). (G, H) Cell migration assay and Matrigel invasion assay were performed to investigate whether NAC treatment could recover cell migration and invasion ability in DDX19A-overexpressing cell lines (SiHa and HCC94) (scale bar: 200μm). Results represent three independent experiments. The results were shown as mean ± SD, ***p < 0.001 by two-tailed Student’s t-test.
The authors apologize for these errors and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
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Keywords: DDX19A, cervical squamous cell carcinoma, metastasis, Nox1, reactive oxygen species
Citation: Jiang Y, Wang B, Li Y, Shen J, Wei Y, Li H, Chen S, Yang H, Zeng F, Liu C, Wang F, He H, Chen Y and Liu J (2022) Corrigendum: DDX19A Promotes Metastasis of Cervical Squamous Cell Carcinoma by Inducing NOX1-Mediated ROS Production. Front. Oncol. 12:914765. doi: 10.3389/fonc.2022.914765
Received: 07 April 2022; Accepted: 25 April 2022;
Published: 09 June 2022.
Edited and reviewed by:
Antonella Zannetti, Institute of Biostructure and Bioimaging (CNR), ItalyCopyright © 2022 Jiang, Wang, Li, Shen, Wei, Li, Chen, Yang, Zeng, Liu, Wang, He, Chen and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Jihong Liu, liujih@mail.sysu.edu.cn; Yong Chen, cy840508@163.com; Huanhuan He, hehh23@mail.sysu.edu.cn
†These authors have contributed equally to this work
‡These authors have contributed equally to this work and share senior authorship