This article is a correction to:
Positive Reciprocal Feedback of lncRNA ZEB1-AS1 and HIF-1α Contributes to Hypoxia-Promoted Tumorigenesis and Metastasis of Pancreatic Cancer
Yan Jin
Zhengming Zhang1†- 1Department of Gastrointestinal Surgery, The First Affiliated Yijishan Hospital of Wannan Medical College, Wuhu, China
- 2Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- 3Department of Emergency Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- 4Department of Pathology, The First Affiliated Yijishan Hospital of Wannan Medical College, Wuhu, China
A Corrigendum on
Positive Reciprocal Feedback of lncRNA ZEB1-AS1 and HIF-1a Contributes to HypoxiaPromoted Tumorigenesis and Metastasis of Pancreatic Cancer
By Jin Y, Zhang Z, Yu Q, Zeng Z, Song H, Huang X, Kong Q, Hu H and Xia Y (2021). Front. Oncol. 11:761979. doi: 10.3389/fonc.2021.761979
In the original article, there was a mistake in Figure 4 as published. The western blot of reference gene (GAPDH) in hypoxia group in Figure 4D was incorrectly used and plotted in this figure. However, this mistake doesn’t affect the results of experiment and understanding of our research on purpose. The corrected Figure 4 appears below.
FIGURE 4
Figure 4 ZEB-1AS1 transcription is regulated by HIF-1α during hypoxia medium. (A) A putative hypoxia-responsive element (HRE) was found in the promoter region of ZEB1-AS1. (B) The expression levels of ZEB1-AS1 (upper) and HIF-1α protein (lower) in BXPC-3/PANC-1 cells were measured after being cultured during normoxia, hypoxia (1%O2), or CoCl2 (concentration of 100 µM under 48 h) at the mRNA and protein levels by qRT-PCR and Western blot analysis, respectively. (C, D) After knockdown of HIF-1α with siRNA, the expression of ZEB1-AS1 was evaluated by qRT-PCR in BXPC-3 and PANC-1 cells under normoxia or hypoxia (upper). Lower diagrams indicated HIF-1α protein levels by Western blot analysis. (E) ChIP assays with anti-HIF-1α antibody were performed to affirm the binding between HIF-1α and HRE of ZEB1-AS1 promoter region in PANC-1 cells under normoxia or hypoxia condition. (F) After being cultured in hypoxia or normoxia, ChIP assays with anti-Pol II antibody were performed to ascertain the binding capacity between Pol II and ZEB1-AS1 promoter region in PANC-1 cells. (G) After knockdown of HIF-1α, the expression of ZEB1-AS1 was shown by FISH assays in PANC-1 cells during normoxia and hypoxia condition. (H) Wild-type ZEB1-AS1 promoter-containing pGL3 reporter vector (WT) or mutant-type ZEB1-AS1 promoter-containing pGL3 reporter vector (MUT) of ZEB1-AS1 promoter sequence firefly luciferase reporter activity in PANC-1 cells transfected with siNC or siHIF-1α and cultured under normoxia or hypoxia conditions were assessed by Renilla luciferase reporter assays after 48 h All data were presented as means ± SD of at least three independent experiments. Values are significant at *p ≤ 0.05 and **p ≤ 0.01 as indicated.
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
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Keywords: pancreatic cancer, lncRNA-ZEB1-AS1, Zeb1, HIF-1α, metastasis, hypoxia
Citation: Jin Y, Zhang Z, Yu Q, Zeng Z, Song H, Huang X, Kong Q, Hu H and Xia Y (2021) Corrigendum: Positive Reciprocal Feedback of LncRNA ZEB1-AS1 and HIF-1α Contributes to Hypoxia–Promoted Tumorigenesis and Metastasis of Pancreatic Cancer. Front. Oncol. 11:821077. doi: 10.3389/fonc.2021.821077
Received: 23 November 2021; Accepted: 24 November 2021;
Published: 07 December 2021.
Approved by:
Frontiers Editorial Office, Frontiers Media SA, SwitzerlandCopyright © 2021 Jin, Zhang, Yu, Zeng, Song, Huang, Kong, Hu and Xia. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Yabin Xia, xiayabin1971@163.com
†These authors have contributed equally to this work