This article is a correction to:
Denatonium as a Bitter Taste Receptor Agonist Modifies Transcriptomic Profile and Functions of Acute Myeloid Leukemia Cells
Valentina Salvestrini1*†
Marilena Ciciarello1†
Valentina Pensato1
Giorgia Simonetti2
Maria Antonella Laginestra1,3Samantha Bruno1Martina Pazzaglia1Elena De Marchi4
Dorian Forte1
Stefania Orecchioni5
Giovanni Martinelli2
Francesco Bertolini5
Simon Méndez-Ferrer5,6,7
Elena Adinolfi4
Francesco Di Virgilio4
Michele Cavo1
Antonio Curti8- 1Department of Experimental, Diagnostic and Specialty Medicine, Policlinico S. Orsola-Malpighi University Hospital, University of Bologna, Bologna, Italy
- 2Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori IRCCS, Meldola, Italy
- 3Laboratory of Experimental Oncology, IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy
- 4Department of Morphology, Surgery and Experimental Medicine, Section of Pathology, Oncology and Experimental Biology, University of Ferrara, Ferrara, Italy
- 5Laboratory of Hematology-Oncology, IRCCS European Institute of Oncology, Milan, Italy
- 6Department of Haematology, Wellcome Trust-Medical Research Council Cambridge Stem Cell Institute University of Cambridge, Cambridge, United Kingdom
- 7Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain
- 8Department of Oncology and Hematology, Institute of Hematology “L. and A. Seràgnoli”, University-Hospital S.Orsola-Malpighi, Bologna, Italy
A Corrigendum on
Denatonium as a Bitter Taste Receptor Agonist Modifies Transcriptomic Profile and Functions of Acute Myeloid Leukemia Cells
Salvestrini V, Ciciarello M, Pensato V, Simonetti G, Laginestra MA, Bruno S, Pazzaglia M, De Marchi E, Forte D, Orecchioni S, Martinelli G, Bertolini F, Méndez-Ferrer S, Adinolfi E, Di Virgilio F, Cavo M and Curti A (2020). Front. Oncol. 10:1225. doi: 10.3389/fonc.2020.01225
In the original article, there was an error in the GEO database accession number. The correct GEO database accession number for gene expression data of denatonium-treated cells is GSE149548.
A correction has been made to the “Methods” section, paragraph 2, and the Data Availability Statement:
Gene expression profiling (GEP)
TAS2R expression was analyzed in 61 AML, 49 from a published dataset (32) and 12 new cases. As validation set, we used also 183 AML samples downloaded from The Cancer Genome Atlas (TCGA) (https://gdc.cancer.gov/about-data/publications/laml_2012)(33). GEP after DEN treatment was performed in 5 newly diagnosed AML samples and THP-1 and OCI-AML3 cell lines. Three independent replicates of each condition were hybridized to Human Clariom S Arrays (Thermo Fisher Scientific) according to the manufacturer’s recommendations. Data quality control, normalization (signal space transformation robust multiple-array average), and supervised analysis were carried out by Expression Console and Transcriptome Analysis Console software, respectively (Thermo Fisher Scientific). For AML cells, data were normalized on vehicle-treated cells before comparison. Genes with a 1.5 fold difference and p ≤ 0.05 were considered for enrichment analyses. Downstream analyses were performed as reported in (32,34), and with Thomson Reuter’s MetaCore software suite (Clarivate Analytics, Philadelphia, PA, USA). Gene expression data of denatonium-treated cells will be publicly available on the GEO database under the accession number GSE149548.
Data Availability Statement
The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found below: the NCBI Gene Expression Omnibus (GSE149548).
The authors apologize for these errors and state that this do not change the scientific conclusions of the article in any way. The original article has been updated.
Keywords: acute myeloid leukemia, bitter taste receptors, denatonium benzoate, bone marrow microenvironment, bitter compounds
Citation: Salvestrini V, Ciciarello M, Pensato V, Simonetti G, Laginestra MA, Bruno S, Pazzaglia M, De Marchi E, Forte D, Orecchioni S, Martinelli G, Bertolini F, Méndez-Ferrer S, Adinolfi E, Di Virgilio F, Cavo M and Curti A (2021) Corrigendum: Denatonium as a Bitter Taste Receptor Agonist Modifies Transcriptomic Profile and Functions of Acute Myeloid Leukemia Cells. Front. Oncol. 11:668460. doi: 10.3389/fonc.2021.668460
Received: 16 February 2021; Accepted: 18 March 2021;
Published: 08 April 2021.
Edited and reviewed by: J. Luis Espinoza, Kanazawa University, Japan
Copyright © 2021 Salvestrini, Ciciarello, Pensato, Simonetti, Laginestra, Bruno, Pazzaglia, De Marchi, Forte, Orecchioni, Martinelli, Bertolini, Méndez-Ferrer, Adinolfi, Di Virgilio, Cavo and Curti. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Valentina Salvestrini, valentin.salvestrin2@unibo.it
†These authors have contributed equally to this work