Early establishment of chloride homeostasis in CRH neurons is altered by prenatal stress leading to fetal HPA axis dysregulation

Corticotropin-releasing hormone (CRH) neurons play an important role in the regulation of neuroendocrine responses to stress. The excitability of CRH neurons is regulated by inhibitory GABAergic inputs. However, it is unclear when GABAergic regulation of CRH neurons is established during fetal brain development. Furthermore, the exact progression of the developmental shift of GABA action from depolarization to hyperpolarization remains unelucidated. Considering the importance of CRH neuron function in subsequent hypothalamic-pituitary-adrenal (HPA) axis regulation during this critical phase of development, we investigated the ontogeny of GABAergic inputs to CRH neurons and consequent development of chloride homeostasis. Both CRH neuron soma in the paraventricular nucleus (PVN) and axons projecting to the median eminence could be identified at embryonic day 15 (E15). Using acute slices containing the PVN of CRF-VenusΔNeo mice, gramicidin perforated-patch clamp-recordings of CRH neurons at E15, postnatal day 0 (P0), and P7 were performed to evaluate the developmental shift of GABA action. The equilibrium potential of GABA (E_GABA) was similar between E15 and P0 and showed a further hyperpolarizing shift between P0 and P7 that was comparable to E_GABA values in adult CRH neurons. GABA primarily acted as an inhibitory signal at E15 and KCC2 expression was detected in CRH neurons at this age. Activation of the HPA axis has been proposed as the primary mechanism through which prenatal maternal stress shapes fetal development and subsequent long-term disease risk. We therefore examined the impact of maternal food restriction stress on the development of chloride homeostasis in CRH neurons. We observed a depolarization shift of E_GABA in CRH neurons of pups exposed to maternal food restriction stress. These results suggest that Cl^– homeostasis in early developmental CRH neurons attains mature intracellular Cl^– levels, GABA acts primarily as inhibitory, and CRH neurons mature and function early compared with neurons in other brain regions, such as the cortex and hippocampus. Maternal food restriction stress alters chloride homeostasis in CRH neurons of pups, reducing their inhibitory control by GABA. This may contribute to increased CRH neuron activity and cause activation of the HPA axis in pups.

Introduction

The hypothalamic-pituitary-adrenal (HPA) axis is a key physiological element in how an organism responds to challenges manifested by either internal or external stressors at any given phase of life (Charmandari et al., 2005). Exposure to a variety of stress leads to activation of corticotropin-releasing hormone (CRH) neurons in the hypothalamic paraventricular nucleus (PVN), which release CRH into portal circulation. This leads to subsequent release of adrenocorticotropic hormone from the anterior pituitary into systemic circulation, culminating in activation of the adrenal cortex, which stimulates synthesis and secretion of cortisol in humans and corticosterone in rodents into the systemic circulation (Ulrich-Lai and Herman, 2009). Thereafter, these glucocorticoids initiate multiple cascades that both limit non-essential energy expenditure and increase energy production to cope with these stressors.

For these reasons, this population of CRH neurons have been the subject of extensive studies investigating their normal physiology and plasticity during stress (Herman and Tasker, 2016). Release of the major inhibitory neurotransmitter γ-aminobutyric acid (GABA) onto CRH neurons from multiple afferent inputs originating in diverse regions of the brain is a key regulator of their activity (Levy and Tasker, 2012). More recent evidence also indicates a GABA-mediated novel mechanism of steady-state CRH release from CRH neuron axon terminals in the median eminence (ME) (Kakizawa et al., 2016; Yesmin et al., 2022). Together, these findings highlight the importance of GABAergic signaling in CRH neuron activity and downstream HPA axis regulation.

The inhibitory action of GABA is closely linked to the phenomenon of chloride homeostasis wherein, following development, low intracellular Cl^– concentrations [Cl^–]_i are maintained by optimal functioning of the K⁺-Cl^– cotransporter KCC2 in mature neurons (Rivera et al., 1999). However, early in development, relatively higher [Cl^–]_i due to suboptimal KCC2 function results in depolarizing GABA action (Ben-Ari, 2002). As KCC2 function develops over time during brain development, the shift from depolarizing to hyperpolarizing GABA action is enacted in most brain regions during the second postnatal week (Watanabe and Fukuda, 2015). Developmental studies examining the formation of hypothalamic nuclei indicate that the birthdate of CRH neurons reportedly peaks around E13 in the rat and mouse brain (Keegan et al., 1994; Markakis and Swanson, 1997), with distinct identifiable structures organizing as early E14–E15 in the PVN. However, what is not addressed is whether the fetal HPA axis becomes functional at this early stage of development. In addition, ontogenesis of CRH neuron-specific KCC2 function regulation of chloride homeostasis, and establishment of mature GABAergic control of the HPA axis remain to be investigated.

The significance of this developmental phase can be further assessed based on a multitude of evidence from both retrospective human studies and experimental prenatal stress animal models (Weinstock, 2008). These reports indicate frequent microscopic and macroscopic alterations in brain structures that culminate in behavioral abnormalities ranging from attention and learning deficits to generalized anxiety and depression in offspring. The dysregulation of both maternal and fetal HPA axes has been proposed as a likely underlying mechanism (Charil et al., 2010). Observations indicating dynamic regulation of KCC2, disruption of [Cl^–]_i, and reverting back to depolarizing GABA action in conditions such as traumatic brain injury, epilepsies, and spinal cord injuries emphasizes their role in the etiology of neuropathologies (Kaila et al., 2014; Fukuda and Watanabe, 2019). In adult rodents, when GABAergic inhibition in CRH neurons is well established, exposure to chronic stress has been shown to affect the plasticity of GABAergic synapses resulting in HPA axis dysregulation (Maguire, 2014). Notably, in response to episodes of stress, GABA action switches from inhibitory to excitatory as a result of KCC2 downregulation (Hewitt et al., 2009; Sarkar et al., 2011). Therefore, the impact of stress on excitability of CRH neurons and overall physiological functioning of the HPA axis is critically dependent on the interplay between KCC2 function and GABA action. It is therefore conceivable to suggest that perinatal stress may affect KCC2 function, the development of GABAergic inhibition onto CRH neurons, and HPA axis regulatory mechanisms in progeny.

In view of this possibility, we here investigated the ontogeny of GABAergic inputs to CRH neurons and consequent development of chloride homeostasis. Furthermore, we examine the impact of perinatal stress in this regulatory sequence.

Materials and methods

Ethical approval

All experimental procedures were in accordance with guidelines issued by the Hamamatsu University School of Medicine on the ethical use of animals for experimentation and approved by the Committee for Animal Care and Use (No. 23-049). All efforts were made to minimize the number of animals used and their suffering.

Animals

The CRF-VenusΔNeo mouse was produced as described previously (Nagai et al., 2002; Itoi et al., 2014; Kono et al., 2017) and kindly gifted from RIKEN BioResource Research Center (BRC No. RBRC09893). CRF-VenusΔNeo mice express Venus under control of the CRF (CRH) promoter. These mice were bred on a C57BL/6J background. Genotyping of CRF-VenusΔNeo mice was carried out by PCR of mouse tail DNA using the following primers: 5’-AGGACGACGGCAACTACAAG-3’ and 5’-TCTCGTTGGGGTCTTTGCTC-3’. In the present study, male CRF-VenusΔNeo mice were placed with female C57BL/6J mice (CLEA Japan, Tokyo, Japan) overnight in a cage. The day when a vaginal plug was identified was defined as embryonic day (E) 0 and gestational day (G) 0 for the mother. Mice were housed with a 12-h light/dark cycle (lights on at 07:00 am) and access to food and water ad libitum unless otherwise noted.

Acute brain slice preparation

Coronal brain slices (300 μm in thickness) including the hypothalamus were obtained from male and female mice at E15–E17 and postnatal days (P) 0–2 and 7–9. Under deep anesthesia with ketamine (150 mg/kg body weight)/xylazine (30 mg/kg body weight), mice were decapitated, and their brains were removed. Slices were made using a Vibrating Microtome 7000 (Campden Instruments, Loughborough, Leicestershire, UK) in an ice-cold oxygenated sucrose solution containing (in mM) 220 sucrose, 120 NaCl, 2.5 KCl, 0.5 CaCl₂, 1.25 NaH₂PO₄, 1 MgCl₂, 26 NaHCO₃, 30 glucose, and 10 MgSO₄ (pH 7.4). Slices were maintained in standard artificial cerebrospinal fluid (ACSF) consisting of (in mM) 126 NaCl, 2.5 KCl, 1.25 NaH₂PO₄, 2 MgSO₄, 2 CaCl₂, 26 NaHCO₃, and 20 glucose (pH 7.4) equilibrated with 95% O₂ and 5% CO₂ at room temperature before recording. For recording, slices were transferred to a recording chamber that was perfused with oxygenated ACSF.

Electrophysiology

Electrophysiological recordings were performed using a MultiClamp 700B amplifier (Molecular Devices, San Jose, CA, USA) and pClamp10 software (Molecular Devices, San Jose, CA, USA). Currents were filtered at 2 kHz and digitized at 10 kHz using DigiData1440A. Data were analyzed offline using Clampfit10 (Molecular Devices, San Jose, CA, USA). To estimate the reversal potential of GABA (E_GABA) of CRH neurons in hypothalamic slices, we performed gramicidin-perforated patch-clamp recording to acquire GABA-evoked responses with native intracellular Cl^– concentrations. CRH neurons expressing Venus were selected under epifluorescent illumination. Patch electrode pipettes (4 to 6 megaOhms) were pulled from borosilicate glass capillaries on a P-97 puller (Sutter Instrument, Novato, CA, USA) and filled with pipette solution composed of 150 mM KCl and 10 mM HEPES (pH 7.2) supplemented with gramicidin (Sigma-Aldrich, St. Louis, MO, USA). Gramicidin was dissolved in methanol to prepare a stock solution of 10 mg/mL and then diluted in pipette solution to a final concentration of 30 μg/mL. Recordings were made in ACSF supplemented with 10 μM CNQX (Sigma-Aldrich, St. Louis, MO, USA), 40 μM D-AP5 (Cayman Chemical, Ann Arbor, MI, USA), 3 μM CGP55845 (Sigma-Aldrich, St. Louis, MO, USA), and 500 nM tetrodotoxin (WAKO, Osaka, Japan) to block AMPA- and NMDA-type glutamate receptors, GABA_B receptors, and voltage-gated Na⁺ channels, respectively. The reversal potential of 100 μM GABA-induced current was measured at −70 mV holding potential (V_H), and 1-sec voltage ramps from −90 to −10 mV applied before and during GABA application. E_GABA was estimated by measuring the voltage at which the I-V relationships before and during GABA application intersected (Saitsu et al., 2016). Series resistance (R_s) was compensated by 70%. Membrane potential values were corrected for the voltage drop across the uncompensated R_s using the following equation: V_corr = V_com – I_clamp × R_s, where V_com is the command potential and I_clamp is the clamp current. To determine E_GABA, these values were plotted as a function of the R_s-corrected membrane potential. GABA puffs (5-sec duration) were applied through a patch pipette approximated to the soma using an IM-300 Programmable Microinjector (Narishige, Tokyo, Japan). To study the effect of GABA on membrane potential, current-clamp recording was performed on CRH neurons of P0-P2 mice in gramicidin-perforated patch mode. The bridge balance circuit was applied while recording voltage changes. Recordings were made in ACSF supplemented with 10 μM CNQX, 40 μM D-AP5, and 3 μM CGP55845. GABA puffs (3-sec duration) were applied through a patch pipette approximated to the soma and GABA-induced potential was recorded. Resting membrane potential (RMP) was calculated from traces at zero current injection (I = 0) levels. The calculated liquid junction potential of −3.6 mV was not corrected because the higher K⁺ ion concentration in the electrode solution compared with the cytosol would be negated by the E_K of approximately +4 mV (Kim and Trussell, 2007).

Immunohistochemistry

Under deep anesthesia with ketamine/xylazine, E15 C57BL/6J mice were transcardially perfused with 4% paraformaldehyde (PFA) in 0.1 M phosphate buffer. Mouse brains were rapidly removed and post fixed for 2 h in 4% PFA at 4°C, followed by 20 and 30% sucrose in phosphate-buffered saline (PBS) at 4°C overnight. Brains were sectioned coronally (30 μm) with a cryostat (HM560R; Zeiss Microm). Subsequently, sections were incubated for 1 h in blocking solution [10% normal goat serum in 0.1% Tween 20 (Sigma-Aldrich, St. Louis, MO, USA) in PBS (PBS-T)] at room temperature before incubation for 48 h at 4°C with guinea pig anti-CRH (1:800; Peninsula Laboratories, San Carlos, CA, USA) and rabbit anti-KCC2 (1:500; Merck Millipore, Burlington, MA, USA) diluted in PBS-T. Subsequently, sections were washed several times with PBS-T and incubated with the following secondary antibodies: Alexa Fluor 488-conjugated goat anti-guinea pig and Alexa Fluor 594-conjugated goat anti-rabbit (1:1,000; Molecular Probes, Eugene, OR, USA). After several washes with PBS-T, the slides were mounted with PermaFluor aqueous mounting medium (Thermo Fisher Scientific, Wilmington, DE, USA) and coverslipped. Slices were imaged using a confocal laser-scanning microscope (FV1000-D, Olympus, Tokyo, Japan).

Food restriction stress

CRF-VenusΔNeo pregnant mice were subjected to 70% food restriction stress from G10 to G18. For the food restriction experiment, daily food consumption of pregnant mice was measured from G10 to G18 and average food consumption per gram of body weight was calculated. Pregnant mice were given a food pellet equal to 70% of their average daily food intake from G10 to G18. Body weights were measured during 70% food restriction. For hormone assays, pregnant mice and fetuses at E18 were used. For electrophysiology experiments, pups were raised by naive surrogate mothers with the same delivery date at P0 and recordings were performed using P0–P2 mice.

Hormone assay

Pregnant mice at E18 were sacrificed by cervical dislocation. Mice were decapitated and the trunk blood was collected into polyethylene tubes containing EDTA-2K (Becton, Dickinson and Company, Franklin Lakes, NJ, USA). Samples were collected between 09:00 and 11:00 am Samples were centrifuged at 800 g for 20 min at 4°C, and the serum was collected and stored at −80°C until further use. As fetuses at E18 provide an insufficient amount of blood, the fetus body was homogenized with a homogenizer in 500 μL of 1 N HCl, followed by centrifugation at 9,100 g for 15 min at 4°C. The resulting supernatant was collected and stored at −80°C. For analysis, 100 μL of each supernatant was lyophilized and reconstituted in 300 μL of radioimmunoassay (RIA) buffer [0.1 M PB (pH 7.4) containing 0.05% NaN₃ and 0.1% Triton X-100]. Corticosterone levels were determined by RIA, as previously reported (Yesmin et al., 2022). Briefly, a 25-μL sample of serum was boiled at 98°C for 5 min. Ice-cold RIA buffer was used for dilution and ¹²⁵I-corticosterone (Institute of Isotopes) was used as the label. A mixture comprising 100 μL of corticosterone standard or sample, 100 μL of corticosterone antiserum, and 100 μL of ¹²⁵I-labeled corticosterone was incubated for 24 h at 4°C. Antibody-bound and antibody-free corticosterone were separated via incubation with 100 μL of a secondary antibody (bovine γ-globulin) and 400 μL of 25% polyethylene glycol, followed by centrifugation at 800 g for 15 min at 4°C. The radioactivity of bound antibodies was counted using a γ-counter (ARC-7010, Aloka, Tokyo, Japan). The assay did not cross-react with other corticosteroids and its sensitivity was 2 pg/tube.

Statistics

All statistical analyses were performed using GraphPad Prism (version 8; GraphPad Software). The data was evaluated for normal distribution based on Shapiro–Wilk test and measure of variance was based on F-Test. If the data was found to be normally distributed, we performed unpaired t-test. When the data failed tests for normality, nonparametric tests for comparison between two groups (Mann–Whitney U test) and multiple groups (Kruskal–Wallis one-way ANOVA followed by Dunn’s multiple comparison test) were used, respectively. Data are presented as mean ± standard error of the mean (SEM) as well as median in the text. When the data is statistically compared using nonparametric test, graphical representation of results in figures show box plot that indicates median, 25 and 75th percentile, and whiskers extending from each end of a box depict the minimum and maximum ranges. The individual data points are shown with dots. The confidence interval for statistics was set at 95% for all data comparisons. P-value < 0.05 is indicated as * and < 0.01 as **. No difference between groups was shown as ns.

Results

E_GABA of CRH neurons during developmental period

The activity of CRH neurons is reportedly regulated by GABA in adult mice (Camille Melón and Maguire, 2016). GABAergic inputs exert inhibitory action on the somata of CRH neurons through activation of GABA_A receptors (GABA_ARs) (Kakizawa et al., 2016). To investigate when GABAergic regulation of CRH neurons is established and the development of chloride homeostasis, we measured E_GABA determined primarily by the Cl^– equilibrium potential [reflecting intracellular [Cl^–]_i (Medina et al., 2014)] using gramicidin-perforated patch-clamp technique, which avoids disruption of native neuronal [Cl^–]_i. Using CRF-VenusΔNeo mice, CRH neurons were identified by Venus fluorescence. We recorded E_GABA of CRH neurons in hypothalamic slices from E15–E17, P0–P2, and P7–P9 mice (Figure 1A). The mean E_GABA of CRH neurons was −61.3 ± 1.4 mV at E15–E17 and −60.6 ± 2.4 mV at P0–P2 (Figure 1B). We observed no significant differences in mean E_GABA values between E15–E17 and P0–P2. The E_GABA of P7–P9 CRH neurons was significantly shifted to more hyperpolarized values (P7–P9: −68.8 ± 1.5 mV) compared to E15–E17 and P0–P2 CRH neurons (p < 0.05 by Kruskal–Wallis test). The corresponding median values for E15-E17 and P0-P2 were −60.8 and −60.7 mV, respectively, whereas for P7-P9 was −67.9 mV. These results suggest that the developmental shift of E_GABA occurs between P0 and P7, and E_GABA of perinatal CRH neurons is hyperpolarized compared to neurons in other brain regions, such as the cortex and hippocampus. During gramicidin-perforated patch-clamp recording, the neuronal membrane occasionally ruptured and spontaneous inhibitory postsynaptic currents (sIPSCs) were observed from E15 CRH neurons when voltage-clamped (V_H) at −70 mV (Figure 1C), suggesting that E15 CRH neurons express synaptic GABA_ARs and receive GABAergic inputs at E15.

FIGURE 1

Figure 1. Developmental change of E_GABA in CRH neurons. Gramicidin-perforated patch-clamp recordings of CRH neurons from E15–E17, P0–P2, and P7–P9 mice. (A) A representative 100 μM GABA-evoked current trace at –70 mV holding potential (upper panel). The duration of GABA application is indicated with horizontal bars. Two 1-sec voltage ramps from –90 to –10 mV were applied before and during 5-sec puff application of GABA; sample I-V curves before (black) and after GABA application (red) (lower panel). E_GABA was estimated from the voltage axis intercept (detailed further in “Materials and methods”). (B) E_GABA of CRH neurons at E15–E17, P0–P2, and P7–P9 (*p < 0.05 by Kruskal–Wallis test; E15–E17: n = 8, P0–P2: n = 11, P7–P9: n = 11; closed circle indicates single cells). The lines in each box depict the lower quartile, median, and upper quartile values. The whiskers extending from each end of a box depict the minimum and maximum ranges. (C) sIPSCs recorded from E15 CRH neurons after the neuronal membrane was ruptured during perforated-patch clamp-recording. Scale bar as shown in the figure inset.

GABAergic inhibitory regulation of CRH neurons is established during early development

Whether activation of GABA_A receptors results in depolarization or hyperpolarization of membrane potential depends on the relationship of E_GABA to the RMP. Next, we performed current-clamp recordings from CRH neurons in hypothalamic slices obtained from P0 mice and examined the effect of GABA on membrane potential changes. Most CRH neurons showed hyperpolarizing responses to brief application of GABA (92%; 13 out of 14 neurons; Figures 2A, C). Only one CRH neuron displayed a depolarizing response to GABA (8%: 1 out of 14 neurons). Two CRH neurons showed spontaneous action potentials that were inhibited by GABA application (Figure 2B). The RMP of CRH neurons at P0–P2 was −41.7 ± 1.3 mV (Figure 2D) and positive to E_GABA. These results suggest that GABA action is already inhibitory at P0–P2.

FIGURE 2

Figure 2. GABAergic inhibitory regulation of CRH neurons is established during early development. (A) Representative current-clamp recordings from P0–P2 CRH neurons in gramicidin-perforated patch mode showing the effect of 100 μM GABA on membrane potential. Five-second application of GABA hyperpolarized (upper panel) or depolarized (lower panel) membrane potential. (B) Application of GABA blocked spontaneous action potentials in P0–P2 CRH neurons (n = 2). (C) Summary of the proportions of each response type (depolarization or hyperpolarization) recorded upon GABA application in P0–P2 CRH neurons (n = 14). (D) The RMP of CRH neurons at P0–P2 (n = 13). Error bars represent SEM. Scale bar as shown in the figure inset.

CRH neuronal somata express KCC2 at E15

The chloride cotransporter KCC2 is essential for GABAergic inhibition. In the cortex and hippocampus, the developmental shift of GABA action from excitatory to inhibitory, which results from KCC2 dependent Cl^– extrusion, occurs between P7 and P14 (Ben-Ari, 2002; Blaesse et al., 2009). Therefore, to examine if KCC2 is expressed in CRH neurons of E15 wild-type (WT) mice, we performed immunohistochemistry for CRH and KCC2. We observed CRH expression in the cell bodies of CRH neurons in the PVN and CRH terminals in the ME (Figures 3A, B), suggesting that CRH neurons already exist in the PVN and CRH axons project to the ME at E15. KCC2 expression in the somata of CRH neurons in the PVN was detected at E15, albeit weakly (Figure 3A), suggesting that it is expressed and functional at this age. In the ME, KCC2 expression in the CRH neuron terminals was not detected, similar to our report that KCC2 is not expressed in the CRH neuron terminals of adult mice (Figure 3B; Kakizawa et al., 2016). Cl^– homeostasis in early developmental CRH neurons attains mature [Cl^–]_i levels with GABA acting in a predominantly inhibitory manner during their early development.

FIGURE 3

Figure 3. KCC2 is expressed in the somata of CRH neurons in PVN of E15–E17 mice. (A) Representative images of PVN from WT E15 mice immunostained for CRH (green) and KCC2 (red). III, third ventricle. White dotted line indicates the PVN with cell bodies of CRH neurons. (B) Representative images of ME from WT E15 mice immunostained for CRH (green) and KCC2 (red). III, third ventricle. White arrowheads indicate CRH neuron terminals in the ME. Scale bars: 50 μm.

Maternal food restriction stress causes a depolarizing shift in E_GABA of CRH neurons in pups

Stress reportedly inhibits KCC2 function in adult CRH neurons of the PVN (Hewitt et al., 2009; Sarkar et al., 2011). Therefore, we examined whether prenatal stress affects chloride homeostasis in CRH neurons during early development. We used a maternal food restriction stress model that subjected pregnant mice to 70% food restriction (FR) from G10 to G18. Prenatal stress began on G10 because it corresponds with development of the fetal central nervous system (Clancy et al., 2001) and minimizes premature termination of the pregnancy as a result of excessive stress. First, we confirmed that FR affects the body weights and corticosterone levels of both the mother and fetus. Body weights of FR mothers were significantly decreased from G13 to G18 compared with control mothers (Figure 4A; p < 0.05, p < 0.01 by Mann–Whitney U test; median value: Control: 28.9 g and FR: 25.8 g at E13, Control: 35.85 g and FR: 30.6 g at E17). Fetal body weights from FR mothers tended to decrease, although not significantly (Figure 4B; ns by Mann–Whitney U test; Control: 1.261 ± 0.03 g; median value: 1.217 g, FR: 1.196 ± 0.02 g; median value: 1.19 g). Maternal serum corticosterone levels were significantly increased in FR mothers compared with control mothers (Figure 4C; p < 0.01 by unpaired t-test). Pups from FR mothers showed increased corticosterone levels compared with control pups (Figure 4D; p < 0.01 by unpaired t-test). These results confirm that FR activated the HPA axis of mothers and pups. To examine the effects of FR on chloride homeostasis in CRH neurons, we examined the effects of FR on E_GABA of CRH neurons from P0–P2 pups. E_GABA of CRH neurons from FR mothers was shifted to more depolarized values (Figures 4E–G; Control: −60.6 ± 2.4 mV, FR: −51.8 ± 2.0 mV; p < 0.01 by unpaired t-test) compared with previously observed E_GABA values from control pups (shown in Figure 1), suggesting that FR alters the chloride homeostasis of CRH neurons and reduces their inhibitory control by GABA. Such alterations may contribute to increased CRH neuron activity and cause activation of the HPA axis.

FIGURE 4

Figure 4. Maternal food restriction stress causes a depolarizing shift in E_GABA of CRH neurons in pups. (A) Body weights of control and FR mothers. The lines in each box depict the lower quartile, median, and upper quartile values. The whiskers extending from each end of a box depict the minimum and maximum ranges (*p < 0.05, **p < 0.01 by Mann–Whitney U test; n = 8 in each group). (B) Body weights of pups from control or FR mothers (ns, not significant; control: n = 12, FR: n = 12; closed circle indicates single cells). (C) Serum corticosterone level of control and FR mothers (**p < 0.01 by unpaired t-test, n = 8 in each group; closed circle indicates single cells). Error bars represent SEM. (D) Fetal corticosterone level of control and FR mothers (**p < 0.01 by unpaired t-test; control: n = 12, FR: n = 12; closed circle indicates single cells). Error bars represent SEM. (E) Representative 100 μM GABA-evoked current traces at –70 mV holding potential in CRH neurons of P0–P2 pups from FR mothers. Currents were recorded under gramicidin-perforated voltage-clamp condition. The duration of GABA application is indicated with horizontal bars. (F) Two 1-sec voltage ramps from –90 to –10 mV were applied before and during 5-sec puff application of 100 μM GABA; sample I-V curves before (black) and after GABA application (red). E_GABA was estimated from the voltage axis intercept (detailed further in “Materials and methods”). (G) Plot of E_GABA of CRH neurons from pups of control or FR mothers (**p < 0.01 by unpaired t-test; control: n = 11, FR: n = 15; closed circle indicates single cells). Error bars represent SEM.

Discussion

Our investigations examining the establishment of GABAergic innervation onto CRH neuron somata and sequela of the developmental shift of GABA action reveal that inhibitory GABA function is established by P0–P2 and undergoes a subsequent hyperpolarizing shift around P7–P9. This result may be facilitated by early KCC2 expression observed at E15 in CRH neurons. In addition, we observed spontaneous firing of CRH neurons at P0–P2 that could be inhibited by GABA application, indicating probable regulation of the fetal HPA axis by GABA function. Furthermore, application of food restriction stress during this critical phase of gestation produced a significant depolarizing shift of E_GABA, suggesting food restriction stress alters chloride homeostasis.

The timing of Cl^– homeostasis maturation varies by brain region and cell type (Watanabe and Fukuda, 2015). Developmental decreases in [Cl^–]_i occur in parallel with maturation of the nervous system. It occurs early in the spinal cord, followed by the hypothalamus and thalamus, and finally the limbic system and cortex. Neurons in the spinal cord and medulla originate mainly between E12–E13, while those in the thalamus, hypothalamus, and amygdala arise from E13–E16. In the neocortex, neurons originate between E16–E18; whereas in the hippocampus, pyramidal neurons arise between E17–E19 and dentate granule cells arise after birth (Wang et al., 2002). The sequence of KCC2 mRNA expression appears to follow the maturation sequence of neurons. KCC2 mRNA expression is detected in the spinal cord at E12, thalamus and hypothalamus at E14, and cortex and hippocampus at P15 (Hübner et al., 2001; Li et al., 2002; Shimizu-Okabe et al., 2002; Wang et al., 2002; Stein et al., 2004). In the hippocampus and cortex, the developmental shift of GABA from excitatory to inhibitory occurs during the second postnatal week (Kahle et al., 2008; Ben-Ari et al., 2012; Watanabe et al., 2019). In the present study, KCC2 was already observed in CRH neurons at E15. E_GABA showed hyperpolarized values that are comparable to E_GABA values in adult CRH neurons and GABA primarily acted as an inhibitory signal early in development, suggesting that CRH neurons mature and function earlier than neurons in the cortex and hippocampus. In addition, sIPSCs could be observed at E15, indicating the establishment of GABAergic synapses onto CRH neuron somata at an early phase of development. As previous reports also observed extrasynaptic GABA_AR-mediated constraint of adult CRH neuron activity, it is probable that ambient GABA in the PVN exerts inhibitory control on these neurons early in development (Lee et al., 2014; Colmers and Bains, 2018). We also report a significant hyperpolarizing shift in E_GABA values at P7–P9 compared with E15–E17 and P0–P2. Interestingly, this phase coincides with the well-characterized stress hyporesponsive period (Vázquez, 1998). We hypothesize that the observed shift in the E_GABA value could be physiologically relevant in limiting the activation of CRH neurons during this period.

Some of the earliest electrophysiological evaluations of adult CRH neurons revealed deinactivation of a specific membrane conductance following hyperpolarization (Tasker and Dudek, 1991). This conductance facilitated generation of small low-threshold potentials in these neurons. Later, this voltage-dependent conductance was used as a distinguishing feature of parvocellular neurons (CRH, somatostatin neurons) in the PVN (Hoffman et al., 1991). More recently, with the advent of transgenic reporter mice, specific targeting of individual populations of cells in the PVN has been accomplished (Sarkar et al., 2011; Wamsteeker Cusulin et al., 2013). Applying a similar strategy for identifying CRH neurons in the developing hypothalamus (Kono et al., 2017), we evaluated the establishment of GABAergic inhibition onto these neurons and the ontogenesis of Cl^– homeostasis.

The development of neuronal membrane excitability of CRH neurons is critical for establishment of the HPA axis. In adult CRH neurons, the slope of frequencies of action potentials fired in response to current injection (f-I curve) displays linearity (Wamsteeker Cusulin et al., 2013). This active property of CRH neurons develops as early as P7 and the density of sodium and potassium channels is further enhanced with age (Melnick et al., 2007), suggesting that CRH neurons can modulate the HPA axis at an early phase. We observed spontaneous action potential firing as early as P0–P2, indicating functional sodium and potassium channels at this age. Therefore, it seems probable that CRH neurons are active early in development and the HPA axis could potentially be established at this stage. Furthermore, the early shift of GABA to hyperpolarizing function may parallel the early development of membrane excitability and act as a restraint for excitation of CRH neurons during the critical phase around birth.

We show that maternal food restriction stress shifted E_GABA to positive values in the CRH neurons of pups. Stress reportedly alters GABA signaling in adult CRH neurons and neurons in other brain regions. Acute restraint stress decreases the total and surface expression of KCC2, causing a depolarizing shift in E_GABA in CRH neurons of the PVN due to dephosphorylation of serine residue 940 (S940) (Hewitt et al., 2009; Sarkar et al., 2011). Chronic social defeat stress causes dephosphorylation at S940 and downregulation of KCC2 in the PVN, and increases plasma corticosterone and depression-like behavior (Miller and Maguire, 2014). In the hippocampus, chronic stress also causes dephosphorylation of KCC2 at S940 and the loss of KCC2 surface expression, coinciding with a depolarizing shift in E_GABA (MacKenzie and Maguire, 2015). In ventral tegmental area GABAergic neurons, acute restraint stress inhibits KCC2 function (Kimmey et al., 2019). Therefore, KCC2 might be downregulated in CRH neurons of pups born to food restricted mothers, which activates CRH neurons.

Exposure to prenatal and early life stress represents a major risk factor for the development of both physical and mental dysfunction later in life (Agorastos et al., 2019). Among the different consequences of exposure to stress on fetal programming, dysregulation of the HPA axis constitutes a critical node capable of affecting multiple organ systems through altered glucocorticoid signaling. Incidentally, evidence from human studies suggests that food restriction stress can trigger growth retardation (Franke et al., 2020; Gantenbein and Kanaka-Gantenbein, 2022; Mousikou et al., 2023). It is hypothesized that food restriction stress triggers multiple cascades involved in anabolic processes during fetal growth. The current consensus understanding underlying this phenomenon stems from observations of reduced nutrient transport via the placenta with key roles assigned to mechanistic target of rapamycin, adiponectin and O-GlcNac transferase signaling (Kramer et al., 2023). However, mechanistic understanding of changes in the fetus responsible for programming of future disease remain unidentified. In particular, development of the brain appears to be compromised, with findings showing reductions in cortical volume, reduced neuronal densities in limbic structures, changes in synaptic plasticity, and aberrant neurotransmission (Kassotaki et al., 2021). In cognizance of our observation of early development of GABAergic inhibitory control of CRH neuron activity, as well as significant depolarization of E_GABA following food restriction stress, we hypothesize that perinatal dysregulation of the fetal HPA axis through stressors may also contribute to maladaptation during this highly plastic phase of brain development. The ensuing cascade of events could be intimately linked to the etiopathology of many neurodevelopmental disorders, and therefore warrants extensive evaluation using multiple animal models of neurodevelopmental disorders.

Data availability statement

The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

Ethics statement

The animal study was approved by the Animal Care and Use Committee of Hamamatsu University School of Medicine. The study was conducted in accordance with the local legislation and institutional requirements.

Author contributions

MW: Conceptualization, Formal analysis, Funding acquisition, Investigation, Writing – original draft, Writing – review & editing. AS: Conceptualization, Formal analysis, Investigation, Writing – original draft, Writing – review & editing. YS: Conceptualization, Supervision, Writing – original draft, Writing – review & editing. AF: Conceptualization, Funding acquisition, Supervision, Writing – original draft, Writing – review & editing.

Funding

The authors declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by Grants-in-Aid for Scientific Research on Innovative Areas [Nonlinear Oscillology, 15H05872 (AF)], Grant-in-Aid for Transformative Research Areas (A) [#21H05687 (AF), #23H04159 (AF)] from Ministry of Education, Culture, Sports, Science, and Technology of Japan, Grants-in-Aid for Scientific Research (B) [#21H02661 (AF)], Grant-in-Aid for Challenging Exploratory Research [17K19682 (AF)], Grants-in-Aid for Scientific Research (C) [#20K06924 (MW), #23K05999 (MW)] from the Japan Society for the Promotion of Science, Research Grants (no. 2138) from The Salt Science Research Foundation (AF), Research grant JERF TENKAN 21012 (MW) and Hamamatsu University School of Medicine Grant-in-Aid (MW).

Acknowledgments

We are grateful to Keiichi Itoi (Tohoku University) for generating CRH Venus mice. We also thank Atsushi Miyawaki (Riken Brain Science Institute) for Venus cDNA. We thank Liwen Bianji (Edanz) (www.liwenbianji.cn/) for basic language editing of a draft of this manuscript.

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

The authors declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

References

Agorastos, A., Pervanidou, P., Chrousos, G. P., and Baker, D. G. (2019). Developmental trajectories of early life stress and trauma: a narrative review on neurobiological aspects beyond stress system dysregulation. Front. Psychiatry 10:118. doi: 10.3389/fpsyt.2019.00118

PubMed Abstract | Crossref Full Text | Google Scholar

Ben-Ari, Y. (2002). Excitatory actions of GABA during development: the nature of the nurture. Nat. Rev. Neurosci. 3, 728–739. doi: 10.1038/nrn920

PubMed Abstract | Crossref Full Text | Google Scholar

Ben-Ari, Y., Khalilov, I., Kahle, K. T., and Cherubini, E. (2012). The GABA excitatory/inhibitory shift in brain maturation and neurological disorders. Neuroscientist 18, 467–486. doi: 10.1177/1073858412438697

PubMed Abstract | Crossref Full Text | Google Scholar

Blaesse, P., Airaksinen, M. S., Rivera, C., and Kaila, K. (2009). Cation-chloride cotransporters and neuronal function. Neuron 61, 820–838. doi: 10.1016/j.neuron.2009.03.003

PubMed Abstract | Crossref Full Text | Google Scholar

Camille Melón, L., and Maguire, J. (2016). GABAergic regulation of the HPA and HPG axes and the impact of stress on reproductive function. J. Steroid Biochem. Mol. Biol. 160, 196–203. doi: 10.1016/j.jsbmb.2015.11.019

PubMed Abstract | Crossref Full Text | Google Scholar

Charil, A., Laplante, D. P., Vaillancourt, C., and King, S. (2010). Prenatal stress and brain development. Brain Res. Rev. 65, 56–79. doi: 10.1016/j.brainresrev.2010.06.002

PubMed Abstract | Crossref Full Text | Google Scholar

Charmandari, E., Tsigos, C., and Chrousos, G. (2005). Endocrinology of the stress response. Annu. Rev. Physiol. 67, 259–284. doi: 10.1146/annurev.physiol.67.040403.120816

PubMed Abstract | Crossref Full Text | Google Scholar

Clancy, B., Darlington, R. B., and Finlay, B. L. (2001). Translating developmental time across mammalian species. Neuroscience 105, 7–17. doi: 10.1016/S0306-4522(01)00171-3

PubMed Abstract | Crossref Full Text | Google Scholar

Colmers, P. L. W., and Bains, J. S. (2018). Balancing tonic and phasic inhibition in hypothalamic corticotropin-releasing hormone neurons. J. Physiol. 596, 1919–1929. doi: 10.1113/JP275588

PubMed Abstract | Crossref Full Text | Google Scholar

Franke, K., Van den Bergh, B. R. H., de Rooij, S. R., Kroegel, N., Nathanielsz, P. W., Rakers, F., et al. (2020). Effects of maternal stress and nutrient restriction during gestation on offspring neuroanatomy in humans. Neurosci. Biobehav. Rev. 117, 5–25. doi: 10.1016/j.neubiorev.2020.01.031

PubMed Abstract | Crossref Full Text | Google Scholar

Fukuda, A., and Watanabe, M. (2019). Pathogenic potential of human SLC12A5 variants causing KCC2 dysfunction. Brain Res. 1710, 1–7. doi: 10.1016/j.brainres.2018.12.025

PubMed Abstract | Crossref Full Text | Google Scholar

Gantenbein, K. V., and Kanaka-Gantenbein, C. (2022). Highlighting the trajectory from intrauterine growth restriction to future obesity. Front. Endocrinol. 13:1041718. doi: 10.3389/fendo.2022.1041718

PubMed Abstract | Crossref Full Text | Google Scholar

Herman, J. P., and Tasker, J. G. (2016). Paraventricular hypothalamic mechanisms of chronic stress adaptation. Front. Endocrinol. 7:137. doi: 10.3389/fendo.2016.00137

PubMed Abstract | Crossref Full Text | Google Scholar

Hewitt, S. A., Wamsteeker, J. I., Kurz, E. U., and Bains, J. S. (2009). Altered chloride homeostasis removes synaptic inhibitory constraint of the stress axis. Nat. Neurosci. 12, 438–443. doi: 10.1038/nn.2274

PubMed Abstract | Crossref Full Text | Google Scholar

Hoffman, N. W., Tasker, J. G., and Dudek, F. E. (1991). Immunohistochemical differentiation of electrophysiologically defined neuronal populations in the region of the rat hypothalamic paraventricular nucleus. J. Comp. Neurol. 307, 405–416.

Google Scholar

Hübner, C. A., Stein, V., Hermans-borgmeyer, I., Meyer, T., Ballanyi, K., and Jentsch, T. J. (2001). Disruption of KCC2 reveals an essential role of K-Cl Cotransport already in early synaptic inhibition Christian. Neuron 30, 515–524. doi: 10.1016/s0896-6273(01)00297-5

PubMed Abstract | Crossref Full Text | Google Scholar

Itoi, K., Talukder, A. H., Fuse, T., Kaneko, T., Ozawa, R., Sato, T., et al. (2014). Visualization of corticotropin-releasing factor neurons by fluorescent proteins in the mouse brain and characterization of labeled neurons in the paraventricular nucleus of the hypothalamus. Endocrinology 155, 4054–4060. doi: 10.1210/en.2014-1182

PubMed Abstract | Crossref Full Text | Google Scholar

Kahle, K. T., Staley, K. J., Nahed, B. V., Gamba, G., Hebert, S. C., Lifton, R. P., et al. (2008). Roles of the cation-chloride cotransporters in neurological disease. Nat. Clin. Pract. Neurol. 4, 490–503. doi: 10.1038/ncpneuro0883

PubMed Abstract | Crossref Full Text | Google Scholar

Kaila, K., Ruusuvuori, E., Seja, P., Voipio, J., and Puskarjov, M. (2014). GABA actions and ionic plasticity in epilepsy. Curr. Opin. Neurobiol. 26, 34–41. doi: 10.1016/j.conb.2013.11.004

PubMed Abstract | Crossref Full Text | Google Scholar

Kakizawa, K., Watanabe, M., Mutoh, H., Okawa, Y., Yamashita, M., Yanagawa, Y., et al. (2016). A novel GABA-mediated corticotropin-releasing hormone secretory mechanism in the median eminence. Sci. Adv. 2:e1501723. doi: 10.1126/sciadv.1501723

PubMed Abstract | Crossref Full Text | Google Scholar

Kassotaki, I., Valsamakis, G., Mastorakos, G., and Grammatopoulos, D. K. (2021). Placental CRH as a signal of pregnancy adversity and impact on fetal neurodevelopment. Front. Endocrinol. 12:714214. doi: 10.3389/fendo.2021.714214

PubMed Abstract | Crossref Full Text | Google Scholar

Keegan, C. E., Herman, J. P., Karolyi, I. J., O’Shea, K. S., Camper, S. A., and Seasholtz, A. F. (1994). Differential expression of corticotropin-releasing hormone in developing mouse embryos and adult brain. Endocrinology 134, 2547–2555. doi: 10.1210/endo.134.6.8194481

PubMed Abstract | Crossref Full Text | Google Scholar

Kim, Y., and Trussell, L. O. (2007). Ion channels generating complex spikes in cartwheel cells of the dorsal cochlear nucleus. J. Neurophysiol. 97, 1705–1725. doi: 10.1152/jn.00536.2006

PubMed Abstract | Crossref Full Text | Google Scholar

Kimmey, B. A., Ostroumov, A., and Dani, J. A. (2019). 5-HT2A receptor activation normalizes stress-induced dysregulation of GABAergic signaling in the ventral tegmental area. Proc. Natl. Acad. Sci. U. S. A. 116, 27028–27034. doi: 10.1073/pnas.1911446116

PubMed Abstract | Crossref Full Text | Google Scholar

Kono, J., Konno, K., Talukder, A. H., Fuse, T., Abe, M., Uchida, K., et al. (2017). Distribution of corticotropin-releasing factor neurons in the mouse brain: a study using corticotropin-releasing factor-modified yellow fluorescent protein knock-in mouse. Brain Struct. Funct. 222, 1705–1732.

Google Scholar

Kramer, A. C., Jansson, T., Bale, T. L., and Powell, T. L. (2023). Maternal-fetal cross-talk via the placenta: influence on offspring development and metabolism. Development 150:dev202088. doi: 10.1242/dev.202088

PubMed Abstract | Crossref Full Text | Google Scholar

Lee, V., Sarkar, J., and Maguire, J. (2014). Loss of Gabrd in CRH neurons blunts the corticosterone response to stress and diminishes stress-related behaviors. Psychoneuroendocrinology 41, 75–88. doi: 10.1016/j.psyneuen.2013.12.011

PubMed Abstract | Crossref Full Text | Google Scholar

Levy, B. H., and Tasker, J. G. (2012). Synaptic regulation of the hypothalamic-pituitary-adrenal axis and its modulation by glucocorticoids and stress. Front. Cell. Neurosci. 6:24. doi: 10.3389/fncel.2012.00024

PubMed Abstract | Crossref Full Text | Google Scholar

Li, H., Tornberg, J., Kaila, K., Airaksinen, M. S., and Rivera, C. (2002). Patterns of cation-chloride cotransporter expression during embryonic rodent CNS development. Eur. J. Neurosci. 16, 2358–2370. doi: 10.1046/j.1460-9568.2002.02419.x

PubMed Abstract | Crossref Full Text | Google Scholar

MacKenzie, G., and Maguire, J. (2015). Chronic stress shifts the GABA reversal potential in the hippocampus and increases seizure susceptibility. Epilepsy Res. 109, 13–27. doi: 10.1016/j.eplepsyres.2014.10.003

PubMed Abstract | Crossref Full Text | Google Scholar

Maguire, J. (2014). Stress-induced plasticity of GABAergic inhibition. Front. Cell. Neurosci. 8:157. doi: 10.3389/fncel.2014.00157

PubMed Abstract | Crossref Full Text | Google Scholar

Markakis, E. A., and Swanson, L. W. (1997). Spatiotemporal patterns of secretomotor neuron generation in the parvicellular neuroendocrine system. Brain Res. Rev. 24, 255–291. doi: 10.1016/S0165-0173(97)00006-4

PubMed Abstract | Crossref Full Text | Google Scholar

Medina, I., Friedel, P., Rivera, C., Kahle, K. T., Kourdougli, N., Uvarov, P., et al. (2014). Current view on the functional regulation of the neuronal K⁺-Cl^– cotransporter KCC2. Front. Cell. Neurosci. 8:27. doi: 10.3389/fncel.2014.00027

PubMed Abstract | Crossref Full Text | Google Scholar

Melnick, I., Pronchuk, N., Cowley, M. A., Grove, K. L., and Colmers, W. F. (2007). Developmental switch in neuropeptide Y and melanocortin effects in the paraventricular nucleus of the hypothalamus. Neuron 56, 1103–1115. doi: 10.1016/j.neuron.2007.10.034

PubMed Abstract | Crossref Full Text | Google Scholar

Miller, S., and Maguire, J. (2014). Deficits in KCC2 and activation of the HPA axis lead to depression like behavior following social defeat. Horm. Stud. 2:2. doi: 10.7243/2052-8000-2-2

Crossref Full Text | Google Scholar

Mousikou, M., Kyriakou, A., and Skordis, N. (2023). Stress and growth in children and adolescents. Horm. Res. Paediatr. 96, 25–33. doi: 10.1159/000521074

PubMed Abstract | Crossref Full Text | Google Scholar

Nagai, T., Ibata, K., Park, E., Kubota, M., and Mikoshiba, K. (2002). A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20, 87–90. doi: 10.1038/nbt0102-87

PubMed Abstract | Crossref Full Text | Google Scholar

Rivera, C., Voipio, J., Payne, J. A., Ruusuvuori, E., Lahtinen, H., Lamsa, K., et al. (1999). The K⁺/Cl^– co-transporter KCC2 renders GABA hyperpolarizing during neuronal maturation. Nature 397, 251–255. doi: 10.1038/16697

PubMed Abstract | Crossref Full Text | Google Scholar

Saitsu, H., Watanabe, M., Akita, T., Ohba, C., Sugai, K., Ong, W. P., et al. (2016). Impaired neuronal KCC2 function by biallelic SLC12A5 mutations in migrating focal seizures and severe developmental delay. Sci. Rep. 6:30072. doi: 10.1038/srep30072

PubMed Abstract | Crossref Full Text | Google Scholar

Sarkar, J., Wakefield, S., MacKenzie, G., Moss, S. J., and Maguire, J. (2011). Neurosteroidogenesis is required for the physiological response to stress: role of neurosteroid-sensitive GABA_A receptors. J. Neurosci. 31, 18198–18210. doi: 10.1523/jneurosci.2560-11.2011

PubMed Abstract | Crossref Full Text | Google Scholar

Shimizu-Okabe, C., Yokokura, M., Okabe, A., Ikeda, M., Sato, K., Kilb, W., et al. (2002). Layer-specific expression of Cl^– transporters and differential [Cl^–]_i in newborn rat cortex. Neuroreport 13, 2433–2437. doi: 10.1097/01.wnr.0000048007

Crossref Full Text | Google Scholar

Stein, V., Hermans-Borgmeyer, I., Jentsch, T. J., and Hübner, C. A. (2004). Expression of the KCl cotransporter KCC2 parallels neuronal maturation and the emergence of low intracellular chloride. J. Comp. Neurol. 468, 57–64. doi: 10.1002/cne.10983

PubMed Abstract | Crossref Full Text | Google Scholar

Tasker, J. G., and Dudek, F. E. (1991). Electrophysiological properties of neurones in the region of the paraventricular nucleus in slices of rat hypothalamus. J. Physiol. 434, 271–293. doi: 10.1113/jphysiol.1991.sp018469

PubMed Abstract | Crossref Full Text | Google Scholar

Ulrich-Lai, Y. M., and Herman, J. P. (2009). Neural regulation of endocrine and autonomic stress responses. Nat. Rev. Neurosci. 10, 397–409. doi: 10.1038/nrn2647

PubMed Abstract | Crossref Full Text | Google Scholar

Vázquez, D. M. (1998). Stress and the developing limbic-hypothalamic-pituitary-adrenal axis. Psychoneuroendocrinology 23, 663–700. doi: 10.1016/s0306-4530(98)00029-8

PubMed Abstract | Crossref Full Text | Google Scholar

Wamsteeker Cusulin, J. I., Füzesi, T., Watts, A. G., and Bains, J. S. (2013). Characterization of corticotropin-releasing hormone neurons in the paraventricular nucleus of the hypothalamus of Crh-IRES-Cre mutant mice. PLoS One 8:e64943. doi: 10.1371/journal.pone.0064943

PubMed Abstract | Crossref Full Text | Google Scholar

Wang, C., Shimizu-Okabe, C., Watanabe, K., Okabe, A., Matsuzaki, H., Ogawa, T., et al. (2002). Developmental changes in KCC1. KCC2 and NKCC1 mRNAs in the rat brain. Dev. Brain Res. 139, 59–66. doi: 10.1016/S0165-3806(02)00536-9

PubMed Abstract | Crossref Full Text | Google Scholar

Watanabe, M., and Fukuda, A. (2015). Development and regulation of chloride homeostasis in the central nervous system. Front. Cell. Neurosci. 9:371. doi: 10.3389/fncel.2015.00371

PubMed Abstract | Crossref Full Text | Google Scholar

Watanabe, M., Zhang, J., Mansuri, M. S., Duan, J., Karimy, J. K., Delpire, E., et al. (2019). Developmentally regulated KCC2 phosphorylation is essential for dynamic GABA-mediated inhibition and survival. Sci. Signal. 12:eaaw9315. doi: 10.1126/scisignal.aaw9315

PubMed Abstract | Crossref Full Text | Google Scholar

Weinstock, M. (2008). The long-term behavioural consequences of prenatal stress. Neurosci. Biobehav. Rev. 32, 1073–1086. doi: 10.1016/j.neubiorev.2008.03.002

PubMed Abstract | Crossref Full Text | Google Scholar

Yesmin, R., Watanabe, M., Sinha, A. S., Ishibashi, M., Wang, T., and Fukuda, A. (2022). A subpopulation of agouti-related peptide neurons exciting corticotropin-releasing hormone axon terminals in median eminence led to hypothalamic-pituitary-adrenal axis activation in response to food restriction. Front. Mol. Neurosci. 15:990803. doi: 10.3389/fnmol.2022.990803

PubMed Abstract | Crossref Full Text | Google Scholar