April Contreras
Dustin J. Hines
Rochelle M. Hines- Department of Psychology, University of Nevada, Las Vegas, Las Vegas, NV, United States
The diversity of inhibitory interneurons allows for the coordination and modulation of excitatory principal cell firing. Interneurons that release GABA (γ-aminobutyric acid) onto the soma and axon exert powerful control by virtue of proximity to the site of action potential generation at the axon initial segment (AIS). Here, we review and examine the cellular and molecular regulation of soma and axon targeting GABAergic synapses in the cortex and hippocampus. We also describe their role in controlling network activity in normal and pathological states. Recent studies have demonstrated a specific role for postsynaptic dystroglycan in the formation and maintenance of cholecystokinin positive basket cell terminals contacting the soma, and postsynaptic collybistin in parvalbumin positive chandelier cell contacts onto the AIS. Unique presynaptic molecular contributors, LGI2 and FGF13, expressed in parvalbumin positive basket cells and chandelier cells, respectively, have also recently been identified. Mutations in the genes encoding proteins critical for somatic and AIS inhibitory synapses have been associated with human disorders of the nervous system. Dystroglycan dysfunction in some congenital muscular dystrophies is associated with developmental brain malformations, intellectual disability, and rare epilepsy. Collybistin dysfunction has been linked to hyperekplexia, epilepsy, intellectual disability, and developmental disorders. Both LGI2 and FGF13 mutations are implicated in syndromes with epilepsy as a component. Advancing our understanding of the powerful roles of somatic and axonic GABAergic contacts in controlling activity patterns in the cortex and hippocampus will provide insight into the pathogenesis of epilepsy and other nervous system disorders.
Introduction
The functional output of the nervous system relies upon coordinated patterns of activity within neuronal circuitry. Neuronal circuits in the cortex and hippocampus are composed of not only excitatory pyramidal cells, but a multitude of diverse interneuron types that express unique complements of proteins and play distinct functional roles (Petilla Interneuron Nomenclature Group et al., 2008). The diversity of inhibitory interneuron signaling allows for multiple levels of modulation of excitatory principal cell firing (Kubota et al., 2016). Interneurons release the neurotransmitter γ-aminobutyric acid (GABA) onto postsynaptic targets, which then binds to GABAA receptors (GABAARs). Diversity is also present in the postsynaptic targets of interneuron types (Figure 1). In particular, interneurons releasing GABA onto the principal cell soma and axon exert powerful control by virtue of proximity to the site of action potential generation at the axon initial segment (AIS; Miles et al., 1996; Klausberger and Somogyi, 2008). As with specialization of the presynaptic interneuron partner, the postsynapse is also specialized by enrichment of GABAAR subtypes. GABAARs are heteropentamers, and those enriched at the postsynapse are most commonly composed of 2α, 2β, and a γ subunit. Some of the postsynaptic specialization of GABAARs is conferred by the α subunit, with α1 containing receptors enriched on the dendrites and soma, and α2 containing receptors enriched on the soma and AIS (Jacob et al., 2008). Further complexity is added due to brain circuits likely relying on unique mechanisms to control synapse targeting, specificity, and molecular specialization. In this review, we examine the cellular and molecular regulation of soma and axon targeting GABAergic synapses in the cortex and hippocampus, as well as clarify their role in controlling network activity in these respective circuits. Because unique mechanisms likely exist in each circuit, we will compare and contrast soma and axon targeting GABAergic synapses in the cortex and hippocampus based on current research. We also examine the role of soma and axon targeting GABAergic synapse dysfunction in pathological states, linking animal phenotypes and human syndromes to key molecular contributors at soma and axon targeting synapses of cortical and hippocampal circuits.
Figure 1. Specialization of inhibitory GABAergic synapse subtypes. Interneurons have molecular specifications which help guide, form, and maintain GABAergic synapses onto distinct areas of the cortical pyramidal cell, which in turn feature molecular specialization in terms of enriched GABAA receptor (GABAAR) subtypes and interacting proteins. Cholecystokinin (CCK) positive basket cells target the soma of pyramidal cells, where the dystrophin glycoprotein complex (DGC) containing dystroglycan and GABAARs containing the α2 subunit are robustly expressed. The CCK positive presynaptic terminal is enriched with Cannabinoid receptor type 1 (CB1). Parvalbumin (PV) positive basket cells target the soma of pyramidal cells enriched with GABAARs containing the α1 subunit anchored by gephyrin. The PV positive presynaptic terminal contains the serotonin 5-HT2A receptor, which is thought to depolarize PV positive basket cells. LGI2 protein is enriched in PV positive basket cells during synaptogenesis, and regulates the formation of these synapses. PV positive chandelier cell cartridges target the axon initial segment (AIS) of pyramidal cells. GABAARs containing the α2 subunit are enriched here, and collybistin interaction plays a key role in AIS localization, although both collybistin and α2 are found at other inhibitory contact sites. The non-secreted protein FGF13 is enriched in PV positive chandelier cells during synaptogenesis, and regulates the formation and maintenance of these synapses.
Soma and Axon Targeting Interneurons
The function of a neuronal circuit relies upon inhibitory interneuron modulation of principal cell activity, with interneurons that contact the perisomatic region exerting powerful control over axonal output of principal cells (Miles et al., 1996). The cortex and hippocampus feature complex circuitry exemplified by interneuron diversity. Interneurons can be classified by their morphology, connectivity, firing pattern, and gene expression pattern. Based on morphology, 16 or more types of interneuron have been distinguished in the hippocampus (Cajal, 1893; Lorente and De Nó, 1934; Parra et al., 1998) while transcriptomic cell typing has identified 23 interneuron types in the cortex (Tasic et al., 2016). Interneurons targeting the perisomatic region generally have a small number of large terminals in comparison to those contacting dendrites. While dendrite-targeting inhibitory synapses can suppress Ca2+ dependent spiking, those contacting the perisomatic region can suppress repetitive discharge of Na+ dependent action potentials (Miles et al., 1996). Despite the morphological and molecular complexity of cortical and hippocampal circuits, the majority of interneurons in these regions express either the neuropeptide cholecystokinin or the calcium binding protein parvalbumin (CCK; PV; Whissell et al., 2015).
CCK positive basket cells target the soma and proximal dendrites of cortical and hippocampal pyramidal cells (Figure 1), and do not appear to innervate the AIS (Panzanelli et al., 2011). Proportions of CCK basket cells express the ionotropic serotonin receptor (5-HT3; Morales and Bloom, 1997) and the metabotropic Cannabinoid receptor type 1 (CB1), which modulate GABA release from the presynaptic terminal (Katona et al., 1999; Lee and Soltesz, 2011). Functionally, CCK positive basket cells provide long-lasting inhibition, modulating cortical and hippocampal cell activity based upon motivation, emotion, and autonomic information from subcortical regions (Buzsáki, 1996; Freund and Katona, 2007).
PV positive interneurons include basket cells which target the soma and proximal dendrites of excitatory pyramidal cells, and chandelier cells whose terminals synapse onto the AIS (Figure 1; Defelipe et al., 1985). PV positive basket cell terminals express the metabotropic serotonin receptor 5-HT2A, with electrophysiological data showing that activation of 5-HT2A depolarizes PV positive GABAergic interneurons (Weber and Andrade, 2010). PV positive basket and chandelier cells are fast-spiking, with the potential to robustly influence the activity of hundreds of pyramidal cells (Hu et al., 2014), and are responsible for the generation of network oscillations in both the cortex and hippocampus (Freund and Buzsáki, 1996). While both CCK positive and PV positive interneurons inhibit the perisomatic region of pyramidal cells, they feature molecular specialization and have unique functional contributions to network activity.
Molecular Specialization of Postsynaptic Sites on the Soma and Axon
Since the identification of distinct interneuron types, research has focused on the unique molecular and functional characteristics of their synaptic specializations. CCK positive somatic terminals are enriched with GABAARs containing the α2/α3 subunits (Nyíri et al., 2001), and the formation and maintenance of CCK terminals is linked to dystroglycan (DG) of the dystrophin glycoprotein complex (DGC; Figure 1; Früh et al., 2016). The DGC is composed of a number of interacting proteins dependent upon tissue type, with brain DGC including dystrophin (or utrophin), syntrophin, dystrobrevin, and DG. Pyramidal cells express DGC in perisomatic clusters postsynaptic to GABAergic terminals (Knuesel et al., 1999; Brünig et al., 2002; Lévi et al., 2002). DG interacts with neurexins to form GABAergic synapses (Sugita et al., 2001), and disruption of the DGC alters synaptic clustering of GABAARs (Knuesel et al., 1999; Vaillend et al., 2010).
Conditional deletion of DG (DG cKO) leads to a loss of the DGC and a modification in GABAAR subunit clustering, but does not prevent the formation of GABAergic terminals (Table 1; Früh et al., 2016). DG cKO mice exhibit a decrease in cluster size of GABAARs containing the α1 subunit, along with an increase in cluster density of GABAARs containing the α2 subunit. Examination of DG cKO mice reveals a specific loss of CCK positive basket cell terminals onto pyramidal cells of the cortex and hippocampal CA1. Induction of DG cKO in mature mice reveals that DG is necessary for the maintenance of CCK positive basket terminals, with the absence of DG leading to a reduction in already formed connections with CCK positive terminals (Früh et al., 2016). The role of DG in the maintenance of CCK positive terminals is independent of neurexin, demonstrated using mice that express the T190M variant of DG, which lacks neurexin binding. Disruption of DG alters functional connectivity of CCK positive terminals, with administration of carbachol to DG cKO slices showing a loss of carbachol-induced increases in inhibitory currents (Früh et al., 2016). Carbachol increases perisomatic inhibitory transmission in pyramidal cells in control slices (Früh et al., 2016), which is mediated by direct excitation of CB1 receptor expressing CCK positive interneurons (Nagode et al., 2014). DG cKO mice also appear to have a reduction in body and brain weight compared to controls (Früh et al., 2016).
Table 1. Overview of key proteins involved in specification and maintenance of soma and axon targeting inhibitory synapses, and implications for disorders of the nervous system.
PV positive cells terminate onto the soma and AIS, with synapses on the AIS enriched with GABAARs containing the α2 subunit (Figure 1; Nusser et al., 1996; Nyíri et al., 2001). Recently, the α2 subunit was shown to have a strong interaction with the collybistin-SH3 domain, but a relatively weak interaction with the gephyrin-E domain (Hines et al., 2018). Conversely, the α1 subunit interaction with the collybistin-SH3 domain is relatively weak, with the gephyrin-E domain interaction being strong. These studies also showed that in vitro, collybistin (CB) and gephyrin compete for interaction with the α2 subunit (Hines et al., 2018). This sets interaction with CB as a possible means of regulating postsynaptic enrichment of α2 subunit containing receptors. To examine this possibility, a substitution mutation was made to introduce the gephyrin-preferring portion of the α1 subunit large intracellular loop into α2 (Gabra2-1). The Gabra2-1 mutation reduces interaction with CB, and results in an increase in total α2, but a decrease in CB expression in both the cortex and hippocampal CA1 (Table 1; Hines et al., 2018). The Gabra2-1 mutation reduces clustering of α2-containing receptors, but does not appear to reduce the overall size or density of inhibitory presynaptic terminals stained by VGAT or GAD65 (Hines et al., 2018). Specific examination of AIS synapses showed a loss of α2-containing receptors, and a loss of VGAT positive terminals opposed to the AIS (Hines et al., 2018). A subset of heterozygous and homozygous Gabra2-1 pups die during postnatal (PN) development, with a peak in mortality at postnatal day 20, and during this time spontaneous seizures are observed. Gabra2-1 mice also show abnormalities in electroencephalogram (EEG) recordings, with elevations in δ-power (Hines et al., 2018).
Molecular Specialization of Soma and Axon Targeting Interneurons
During synaptogenesis, contact between opposite yet complementary pre- and post-synaptic terminals is essential for proper circuit formation. In addition to postsynaptic specializations on pyramidal cells, recent papers have identified molecular specialization of presynaptic interneuron subtypes critical for their postsynaptic targeting. Cell sorting of interneurons during peak synaptogenesis, followed by RNA-sequencing and whole-transcriptome analyses, has recently identified molecular programs for synaptogenesis specific to soma, axon, and dendrite targeting interneurons (Favuzzi et al., 2019). Gene ontology analysis showed that the most enriched genes are those belonging to synaptic membrane compartments and processes that contribute to synaptogenesis, which were not enriched in mature cortex (Favuzzi et al., 2019). Lgi2, a member of the leucine-rich glioma inactivated protein gene family, was identified as a chief regulator for the establishment of perisomatic inhibitory synapses by a population of PV positive basket cells (Figure 1; Favuzzi et al., 2019). Lgi2 encodes a secreted protein (LGI2) that consists of leucine-rich repeat and epilepsy-associated/epitempin (EPTP) domains. Prior studies have implicated the related family member LGI1 in maturation of excitatory synapses (Senechal et al., 2005; Kegel et al., 2013). LGI proteins have been shown to interact with a disintegrin and metalloprotease (ADAM) proteins (Seppälä et al., 2011).
Through cell sorting Fgf13 was identified as a candidate for AIS-targeting chandelier synapses (Figure 1; Favuzzi et al., 2019). Fgf13 is a member of the fibroblast growth factor gene family, which encodes proteins (FGFs) critical for development (Wu et al., 2012; Pablo et al., 2016). Unlike many FGF family members, FGF13 is non-secretory and functions independent of FGF receptors. FGF13 has been shown to be a microtubule stabilizing protein enriched in the growth cones of cortical cells (Wu et al., 2012). FGF13 is also known to limit localization of voltage-gated sodium channels to the somatodendritic compartment of principal neurons, while FGF14 promotes localization to the proximal axon (Pablo et al., 2016).
To investigate the role of Lgi2 and Fgf13, interneuron cell type-specific Cre-driver lines were combined with adeno-associated virus (AAV) vectors carrying miR-based short-hairpin RNAs (Favuzzi et al., 2019). Cell-specific down-regulation of Lgi2 and Fgf13 led to a decrease in density of presynaptic inputs from interneurons expressing the short-hairpin RNAs. A decrease in somatic inhibitory synapses made by PV positive basket cells was observed upon down-regulation of Lgi2 at P2 (Table 1; Favuzzi et al., 2019). ADAM22, the proposed postsynaptic partner of expressed LGI2 was also shown to be colocalized with gephyrin clusters on the soma, opposite GAD-65+ terminals (Favuzzi et al., 2019). Interestingly, Fgf13 deficient PV positive chandelier cells showed axonal disorganization in addition to a loss of AIS innervation when down regulation was induced at P2 (Table 1; Favuzzi et al., 2019). Axonal disorganization itself may contribute to the decrease in AIS innervation observed, although this may also represent a dual role for FGF13. Interestingly, chandelier cell synaptic boutons were decreased in the absence of axonal disorganization if Fgf13 was down-regulated after P14, confirming that expressed FGF13 also plays a role in maintenance of chandelier cell contacts onto the AIS, after the axon has reached its target (Favuzzi et al., 2019).
Implications of Gabaergic Synapses on the Soma and Axon in Disorders of the Nervous System
The coordination of excitatory principal cell firing relies on interneuron function, and dysregulation of soma and axon targeting interneurons has been identified in disorders of the nervous system (DeFelipe et al., 1993; Rubenstein and Merzenich, 2003; Ali Rodriguez et al., 2018). Disruption of neuronal DG directly impacts the maintenance of CCK positive basket terminals onto pyramidal cell somas, leading to impaired CCK positive interneuron mediated neurotransmission and functional connectivity. Mutations in DGC components such as dystrophin are the most common cause of muscular dystrophies, which are movement disorders characterized by a robust degeneration of muscle tissue (Table 1; McNally and Pytel, 2007). Muscular dystrophies with neurological aberrations can be caused by varying genetic mutations, and are associated with a lack of available glycosylated DG (Table 1; Brancaccio, 2005; Barresi and Campbell, 2006). Varying ranges of intellectual disability have been identified in individuals with muscular dystrophies, and cognitive deficits are associated with neuronal DG alterations (Knuesel et al., 1999; Moore et al., 2002; Daoud et al., 2009; Desguerre et al., 2009; Vaillend et al., 2010).
Disruption in inhibitory signaling mediated by PV positive cells on a global level has been associated with neurodevelopmental disorders (Ali Rodriguez et al., 2018). Schizophrenia, through post-mortem studies and in animal models, has been associated with soma and axon targeting inhibitory synapses (Lewis et al., 2008, 2012; Hines et al., 2013). Autism spectrum and related disorders such as Angelman syndrome and Rett syndrome have also been linked to PV cell dysfunction, and notably these disorders have a high incidence of epilepsy (Table 1; Kalscheuer et al., 2009; Shimojima et al., 2011; Ali Rodriguez et al., 2018). Mutations in the GABAAR subunit genes have been implicated in genetic epilepsies (Baulac et al., 2001; Wallace et al., 2001; Hines et al., 2018). The gene encoding the α2 subunit (GABRA2) was identified as one of the most likely biological epilepsy genes in a recent genome-wide mega-analysis (The International League Against Epilepsy Consortium on Complex Epilepsies, 2018). In the Gabra2-1 animal model, altered clustering of GABAARs containing the α2 subunit led to developmental seizure and mortality, as well as anxiety-like phenotypes (Hines et al., 2018). In humans, mutations in the gene encoding collybistin (ARHGEF9), lead to hyperekplexia syndromes that include intellectual disability and mental retardation (Table 1; Shimojima et al., 2011; Striano and Zara, 2017). ARHGEF9 mutations are also associated with epilepsies and anxiety in humans (Kalscheuer et al., 2009; Wang et al., 2018).
LGI2 and FGF13 dysfunction have also been linked to epilepsy. LGI1 mutations account for about half of Autosomal Dominant Lateral Temporal lobe Epilepsy (ADLTE; Kalachikov et al., 2002). Mutations in LGI2 have also been associated with an epileptic phenotype, especially that of canine Benign Familial Juvenile Epilepsy (Table 1; Fukata et al., 2010; Seppälä et al., 2011). LGI2 is also a leading candidate for mutations in the 4p15 region thought to be responsible for Partial Epilepsy with Pericentral Spikes (PEPS; Kinton et al., 2002; Limviphuvadh et al., 2010). Mutations in FGF13 are linked to Genetic Epilepsy and Febrile Seizures Plus (GEFS+; Guillemot and Zimmer, 2011; Puranam et al., 2015), as well as Börjeson-Forssman-Lehmann syndrome, which is a rare X-linked disorder characterized by intellectual disability, obesity, seizures, hypogonadism, and distinctive facial features (Table 1; Malmgren et al., 1993; Gecz et al., 1999).
Discussion
The modulation of excitatory pyramidal cells by GABAergic interneurons is determined by interneuron diversity, allowing for the complex computations performed by these neuronal circuits (Tremblay et al., 2016). Of the many interneuron subtypes, those that release GABA onto the soma and axon can powerfully influence and fine-tune neuronal activity (Miles et al., 1996). CCK positive cells target the soma and proximal dendrites of pyramidal cells in cortex and hippocampus, and rely on DG for targeting of α2/α3 containing GABAARs to postsynaptic sites on the hippocampal pyramidal cell soma (Früh et al., 2016). Interestingly, clustering of GABAARs at sites opposing CCK positive terminals appears to be independent of DG, as α2 containing GABAARs still cluster in DG cKO mice in the absence of CCK positive terminals (Früh et al., 2016). Consistent with this, gephyrin clustering was also unaffected in DG cKO, while CB was not assessed (Früh et al., 2016). It remains unclear how gephyrin might selectively stabilize specific subtypes of GABAARs at postsynaptic sites despite its ubiquitous presence, but leading hypotheses point to posttranslational modification (Ghosh et al., 2016), or subtleties in multi-protein complex arrangements (Saiepour et al., 2010). Somatic contacts from CCK positive basket cells were unaffected by a DG mutation that interferes with neurexin binding (Früh et al., 2016), encouraging the exploration of possible novel presynaptic partners in CCK positive terminals that are needed for transsynaptic signaling during synapse formation and maintenance at sites contacting the soma.
PV positive chandelier cells terminate onto the AIS which is enriched with GABAARs containing the α2 subunit, and the high affinity interaction between α2 and CB appears essential in this enrichment and in the maintenance of these synapses on cortical pyramidal cells (Hines et al., 2018). Despite evidence of a preferential interaction between α2 and CB, as well as a preferential role of this complex at AIS synapses onto cortical pyramidal cells, several points remain to be clarified. Although the interaction strength between α2 and gephyrin was comparatively weak, a prominent effect of α2 KO is a loss of gephyrin clustering at perisomatic synapses onto CA1 pyramidal cells (Panzanelli et al., 2011). The molecular mechanism regulating this loss of gephyrin remains unclear but may relate to an indirect interaction between α2 and gephyrin. Although the interaction between α2 and CB appears critical for AIS synapses in the cortex, α2 and CB are well known to be present at other synapse types. Conversely, α1 and α3 containing receptors can also be detected at AIS synapses, particularly in the hippocampus and amygdala (Gao and Heldt, 2016), thus analysis of the impact of the Gabra2-1 mutation on AIS synapses in other brain regions is needed.
Developmental RNA-seq focusing on the period of peak inhibitory synapse formation demonstrated that distinct types of interneurons rely on a largely unique complement of molecular programs for the specific subcellular contact sites that they establish (Favuzzi et al., 2019). During synaptogenesis, genes involved in targeting and matching PV positive interneuron axons to their postsynaptic targets include expression of Lgi2 for basket cells, and Fgf13 for chandelier cells (Favuzzi et al., 2019). Details of how the expressed proteins function at presynaptic terminals during synaptogenesis remain to be uncovered, including further illumination of specific interacting partners and effectors. In addition to the genes that were characterized in more detail, a number of others were identified to have relatively specific regulated expression patterns related to synapse formation (Favuzzi et al., 2019). Many of these were genes encoding adhesion proteins, as well as extracellular components such as proteins that make up the peri-neuronal net (Favuzzi et al., 2019). Investigation into some of the other candidates will allow for more detailed illumination of the steps involved in building each unique type of inhibitory contact on the soma and axon.
In general, studies have yet to replicate or contrast these mechanisms in regulating somatic and axon targeting inhibitory synapse formation, maintenance, and function in distinct brain circuits. As a point of comparison, the cerebellum has a more limited repertoire of cell types, and the cellular and molecular specialization of soma and axon targeting interneurons in the cerebellum is relatively well established (Somogyi and Hámori, 1976; Somogyi et al., 1983; Li et al., 1992; Ango et al., 2004). In the cerebellum, principal Purkinje cells receive GABAergic innervation from stellate and basket cells. Stellate cells target dendritic domains, while basket cells innervate the perisomatic region and ensheath the AIS (pinceau formation) of Purkinje cells. Guidance of the basket cell axon to the Purkinje AIS is mediated by Semaphorin 3A and its receptor neuropilin-1, which interacts directly with the adhesion molecule NF186 at the AIS target (Cioni et al., 2013; Telley et al., 2016). Maturation of the Purkinje AIS and pinceau formation relies upon neurofascin interaction with Ankyrin-G (Ango et al., 2004; Zonta et al., 2011; Buttermore et al., 2012). Somatic synapses on Purkinje cells are enriched with both α1 and α3 containing GABAARs (Fritschy et al., 2006). The maintenance of these synapses does not depend on α1 expression (Fritschy et al., 2006), and α1 expression on the Purkinje soma is maintained in CB knockout in the absence of gephyrin (Papadopoulos et al., 2007), leaving the mechanisms required to build the postsynaptic compartment of somatic synapses of Purkinje cells unclear. Also of interest, another intracellular FGF family member, FGF14, is localized to the AIS, and has been implicated in Purkinje neuron excitability by impacting voltage gated Na+ channel kinetics (Goldfarb et al., 2007; Xiao et al., 2013); thus distinct FGFs may play unique but complementary roles at the AIS. Additional studies should compare and contrast the contributions of this subclass of FGFs in formation and maintenance of axon targeting synapses across multiple circuits.
The function of inhibitory synapses on the soma and axon is perhaps best illustrated by the effects observed upon mutation (Table 1). Epilepsy is an interesting common thread among soma and axon targeting inhibitory synapse gene syndromes. Given the role of soma and axon targeting interneurons in coordinating principal cell activity, discoordination of neuronal activity patterns is a logical extension. Yet further studies are needed to understand the distinction between disrupting specific synapse subtypes and functional implications for circuit activity. Examination of animal models for these disorders focusing on abnormalities in the development and maintenance of specific inhibitory synapse subtypes will be helpful in confirming a selective contribution. Identification of specific synapse subtypes, along with key molecular players at these sites may allow the development of molecular and pharmacological interventions that more precisely modulate the development and maintenance of specific inhibitory synapse subtypes. Further knowledge of specific synapse subtypes in these disorders will ultimately aid with the refinement or development of novel therapeutic strategies.
Author Contributions
AC, DH, and RH wrote the manuscript. RH conceived the work and prepared the figure.
Funding
This work was supported by the UNLV University Libraries Open Article Fund.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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Keywords: GABAergic synapse development, epilepsies and epileptic syndromes, cholecystokinin, parvalbumin, interneuron, GABAA receptor subunits, somatic inhibitory synapse, axon initial segment inhibitory synapse
Citation: Contreras A, Hines DJ and Hines RM (2019) Molecular Specialization of GABAergic Synapses on the Soma and Axon in Cortical and Hippocampal Circuit Function and Dysfunction. Front. Mol. Neurosci. 12:154. doi: 10.3389/fnmol.2019.00154
Received: 29 March 2019; Accepted: 31 May 2019;
Published: 26 June 2019.
Edited by:
Shiva Tyagarajan, University of Zurich, SwitzerlandReviewed by:
Marco Sassoè-pognetto, University of Turin, ItalyYuchio Yanagawa, Gunma University, Japan
Copyright © 2019 Contreras, Hines and Hines. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Rochelle M. Hines, cm9jaGVsbGUuaGluZXNAdW5sdi5lZHU=