Danae Campos-Melo
Danny Galleguillos†
Katia Gysling
María E. Andrés- Nucleus Millennium in Stress and Addiction, Department of Cellular and Molecular Biology, Faculty of Biological Sciences, Pontificia Universidad Católica de Chile, Santiago, Chile
The Nur transcription factors Nur77 (NGFI-B, NR4A1), Nurr1 (NR4A2), and Nor-1 (NR4A3) are a sub-family of orphan members of the nuclear receptor superfamily. These transcription factors are products of immediate early genes, whose expression is rapidly and transiently induced in the central nervous system by several types of stimuli. Nur factors are present throughout the hypothalamus-pituitary-adrenal (HPA) axis where are prominently induced in response to stress. Drugs of abuse and stress also induce the expression of Nur factors in nuclei of the motivation/reward circuit of the brain, indicating their participation in the process of drug addiction and in non-hypothalamic responses to stress. Repeated use of addictive drugs and chronic stress induce long-lasting dysregulation of the brain motivation/reward circuit due to reprogramming of gene expression and enduring alterations in neuronal function. Here, we review the data supporting that Nur transcription factors are key players in the molecular basis of the dysregulation of neuronal circuits involved in chronic stress and addiction.
Introduction
The transcription factors Nur77 (NGFI-B, NR4A1) (Hazel et al., 1988; Milbrandt, 1988), Nurr1 (NR4A2) (Law et al., 1992) and Nor-1 (NR4A3) (Ohkura et al., 1996) are orphan members of the nuclear receptor superfamily and together conform the Nur subfamily. Nur transcription factors, as members of the nuclear receptor superfamily, share their classic structural organization (Figure 1A) encompassing: (a) a non-conserved N-terminal region containing the transcriptional activation function-1 (AF-1), (b) a conserved DNA binding domain (DBD) located in the middle of the proteins, with 90.2% of amino acid sequence identity among all rat Nur factors, and (c) a moderately conserved C-terminal domain, which encloses the ligand-binding domain (LBD) and the ligand-dependent transcriptional activation function 2 or AF-2 (Giguere, 1999). Although Nur transcription factors have a well-recognized LBD structure, their transcriptional activity is not regulated by endogenous ligands as it is for steroid nuclear receptors (Benoit et al., 2004). Crystallographic studies show that the putative ligand-binding pocket of Nurr1 and Nur77 LBDs are filled with side chains of large hydrophobic amino acids, which keep the LBD in a transcriptionally active conformation (Wang et al., 2003; Flaig et al., 2005). Since the binding of ligands does not trigger the transcriptional activity of Nur factors, changes of their expression levels and post-translational modifications appear keys to regulate their activity.
Figure 1. Scheme of Nur factors structure and DNA-binding elements. (A) Nur factors common structure, AF: activation function domain; DBD: DNA Binding Domain; LBD: Ligand Binding Domain. (B) Nur factors DNA binding elements. RXR: retinoic-X-receptor; 9-cis RA: 9-cis retinoic acid.
Nur factors behave as immediate early genes, and as such, their mRNA expression is induced independent of protein synthesis in several cell types by multiple kinds of stimuli (Williams and Lau, 1993; Maruyama et al., 1995; Satoh and Kuroda, 2002; Maxwell and Muscat, 2006). Nur factors show some basal expression in specific nuclei of the rodent brain (Zetterstrom et al., 1996b). However, remarkable fast and high induction has been observed for Nur factors in selected brain nuclei or dissociated neurons after physiological, chemical or toxic stimulation (Chan et al., 1993; Honkaniemi et al., 1994; Jacobs et al., 1994; Umemoto et al., 1994; Svenningsson et al., 1995; Imaki et al., 1996; Svenningsson and Fredholm, 1997; Tang et al., 1997; Xing et al., 1997; Honkaniemi and Sharp, 1999; Brosenitsch and Katz, 2001; Ojeda et al., 2003; Maheux et al., 2005). These data strongly support the notion that Nur factors have homeostatic functions and, functioning as immediate-early gene, can also serve as rapid signaling system.
To exert their function as transcriptional regulators, Nur factors bind to nerve-growth-factor inducible gene B (NGFI-B)-responsive element (NBRE) (A/TAAAGGTCA) (Wilson et al., 1991) as monomers (Figure 1B) (Wilson et al., 1993a; Paulsen et al., 1995), and to Nur-responsive element (NurRE) (inverted repeated of NBRE-related octanucleotide, AAATG/AC/TCA) (Philips et al., 1997) as homodimers or heterodimers between Nur factors (Philips et al., 1997; Maira et al., 1999). Nur77 and Nurr1, but not Nor-1, can also form heterodimers with RXR retinoid receptor (Perlmann and Jansson, 1995; Zetterstrom et al., 1996a). These heterodimers activate transcription through NBRE elements, where the Nur component binds to the NBRE element, or through DR5 elements (two direct repeats of the consensus nuclear receptor binding motif separated by five nucleotides, GGTTCAnnnnnAGGTCA). In the case of DR5 elements, both nuclear receptors bind to the DNA (Perlmann and Jansson, 1995). In addition, Nur factors can regulate transcription indirectly by binding to another transcription factor. For instance, Nurr1 represses transcription of inflammatory genes in microglia indirectly by forming a complex with NF-kB transcription factor (Saijo et al., 2009).
Increasing reports show that Nur factors transcriptional activity is regulated by post-translational modifications (Figure 2). Phosphorylation of Nur77 by extracellular-signal regulated kinase 2 (ERK2) is required for its transcriptional activity in corticotrophs (Kovalovsky et al., 2002). Similarly, Nurr1 phosphorylation by mitogenic-activated protein kinases increases its transcriptional activity (Nordzell et al., 2004; Sacchetti et al., 2006; Zhang et al., 2007). Phosphorylation of Nur factors plays an important role also in their subcellular translocation and Nur-dependent induction of apoptosis (Katagiri et al., 2000; Slagsvold et al., 2002; Wingate et al., 2006). The post-translational modification SUMOylation has appeared as an important regulatory pathway of Nurr1 transcriptional activity (Galleguillos et al., 2004; Saijo et al., 2009; Arredondo et al., 2013). All Nur factors harbor SUMO consensus motifs in their sequences, but only Nurr1 SUMOylation by SUMO2 and SUMO3 has been demonstrated. SUMOylation of the N-terminal domain of Nurr1 with SUMO2 reduces its transcriptional activity in promoters harboring more than one NBRE element (Arredondo et al., 2013). Also, SUMOylation with SUMO2 or SUMO3 of the C-terminal of Nurr1 is required to bind the corepressor CoREST and repress pro-inflammatory gene expression (Saijo et al., 2009). Recent work has shown that Nur factors turnover and transcriptional activity are regulated by acetylation (Kang et al., 2010) and ubiquitination (van Tiel et al., 2012; Alvarez-Castelao et al., 2013).
Figure 2. Nur factors are post-translational modified. (A) Phosphorylation, (B) SUMOylation, and (C) Acetylation and ubiquitination.
Nur factors are associated to several functions (Maxwell and Muscat, 2006). Some of the functions are exclusive of one factor, like the essential role of Nurr1 in the induction and maintenance of midbrain dopamine neurons (Zetterstrom et al., 1997; Castillo et al., 1998; Saucedo-Cardenas et al., 1998; Kadkhodaei et al., 2009), and Nor-1 requirement for normal embryonic development (Deyoung et al., 2003), inner ear (Ponnio et al., 2002), and hippocampus development (Ponnio and Conneely, 2004). Increasing evidence indicates that all Nur factors play significant roles in inflammatory (McMorrow and Murphy, 2011; van Tiel and de Vries, 2012) and oncogenic processes (Mohan et al., 2012). Increased expression levels of Nur factors have been observed in several types of cancer cell lines and tumors, although the opposite has also been reported. Collected data suggest that Nur77 behaves as a pro-oncogenic factor (Lee et al., 2011). However, the double knockout of Nur77 and Nor-1 induced a fatal acute myeloid leukemia in mice (Mullican et al., 2007), indicating that these nuclear receptors may also play a role as tumor suppressors (Mullican et al., 2007). The apparent controversy or dual role of Nur factors as tumor suppressors and/or pro-oncogenic factors could be explained by a dual role in transcription, behaving as transcriptional activators or repressors. Since Nur factors are transcriptionally active in their native form, less attention has been paid to their role as potential transcriptional repressors. Interestingly, there are several reports showing the interaction of Nur factors with transcriptional corepressors. For example, we showed that PIASγ interacts and represses Nurr1-dependent transcriptional activity (Galleguillos et al., 2004). Nurr1 also interacts with the transcriptional repressors SMRT (Lammi et al., 2008; Jacobs et al., 2009) and CoREST (Saijo et al., 2009). The interaction with SMRT maintains Nurr1 in a transcriptional repressive complex impeding the induction of its dopaminergic target genes (Jacobs et al., 2009). It has also been shown the interaction between Nur77 and SMRT (Sohn et al., 2001). Regarding the role of Nur77 and Nor-1 repressing the expression of target genes, it was shown that abrogation of these transcription factors correlates with an increased expression of MYC oncogene (Boudreaux et al., 2012). In addition, it was demonstrated that MYC is a direct target gene of Nur77/Nor-1, whose expression was strongly repressed in a Nur DNA-binding dependent way (Boudreaux et al., 2012). Nur77 and Nor-1 play also an important role triggering apoptosis in several cell types. Interestingly, this effect is due to the translocation of Nur77/Nor-1 from the nuclei to the mitochondria, where Nur77 triggers cytochrome c release and apoptosis (Li et al., 2000). Through this mechanism, Nur77 and Nor-1 play a central function in the clonal deletion of autoreactive thymocytes (Sohn et al., 2007). Nur77 and Nor-1 colocalize in several cell types, including CNS neurons, and apparently they replace each other in some functions. This colocalization of Nur77 and Nor-1 explain the lack of deleterious effects in the single knockout mice, while lack of both induces a catastrophic deregulation in the immune system. Similarly, colocalization of Nur77 with Nor-1 in the CNS may explain an apparent lack of strong effect of each knockout in the stress and rewarding systems of the brain.
Nur Transcription Factors and Dopaminergic Transmission
Histological and neurochemical evidence supports the idea that Nur transcription factors are closely associated with dopamine neurotransmission (Figure 3, Table 1). Nur77 and Nor-1 are expressed in neurons of the striatum, the nucleus accumbens (NAc) and the prefrontal cortex (PFC) (Xiao et al., 1996; Zetterstrom et al., 1996b; Gervais et al., 1999; Werme et al., 2000a,b; Davis and Puhl, 2011), all target nuclei of dopaminergic neurons originated in the ventral midbrain. In these nuclei, a strong control of Nur77 and Nor-1 expression is induced by the stimulation of the dopamine neurons (Chergui et al., 1997); by the administration of dopamine D2 receptor agonists and antagonists (Gervais et al., 1999; Beaudry et al., 2000; Werme et al., 2000b; Langlois et al., 2001; Maheux et al., 2005); by drugs of abuse (Werme et al., 2000a; St-Hilaire et al., 2003b) and after dopamine denervation. Collected data indicate that Nur77 expression is under a tonic inhibitory control exerted by physiological dopamine basal levels through D2 receptor. Acute administration of dopamine D2 agonists decreases Nur77 mRNA levels in the striatum (Gervais et al., 1999), while the opposite is observed after acute dopamine D2 antagonist administration in the striatum, NAc and PFC (Beaudry et al., 2000; Langlois et al., 2001; Maheux et al., 2005). Interestingly, D2 antagonist-dependent induction of Nur77 expression in the striatum and NAc core is preserved after chronic treatment with D2 ligands (Beaudry et al., 2000; Werme et al., 2000b; Mahmoudi et al., 2009, 2013), suggesting that this transcription factor mediates adaptive changes to long-term repetitive dopamine variations (Levesque and Rouillard, 2007).
Figure 3. Expression of Nur factors in the in the motivation/reward circuit. Nurr1 is expressed in dopamine neurons of the SN/VTA and Nur77 and Nor-1 are expressed in dopamine-receptive GABAergic neurons of the striatum/NAc. GABAergic projecting neurons in the striatum/NAc are segregated in two populations that express either D1 or D2 receptors (Valjent et al., 2009).
Table 1. Nur transcription factors and dopaminergic neurotransmission.
Studies using dopamine denervation and Nur77 knockout mice have been instrumental to reveal the role of Nur77 as a master factor for adaptations induced by dopaminergic neurotransmission changes. Full lesion of dopamine nigro-striatal pathway and D2 antagonist administration produce a significant up-regulation of both Nur77 and enkephalin mRNA in enkephalin positive neurons of the striatum (Beaudry et al., 2000; St-Hilaire et al., 2003a, 2005). Enkephalin up-regulation induced by D2 antagonists or by dopamine denervation is severely impaired in Nur77 knockout mice (Ethier et al., 2004; St-Hilaire et al., 2006). Moreover, the normalization of enkephalin levels induced by L-DOPA treatment of dopamine denervated animals is not observed in Nur77 knockout mice (St-Hilaire et al., 2006), indicating that Nur77 is essential for this adaptive effect. Interestingly, a recent publication suggests that the D2 control over Nur77 expression in the striatum is due to presynaptic D2 receptors located on glutamatergic terminals coming from the cortex (Maheux et al., 2012). Metabotropic mGlu5 receptor antagonist suppressed D2 antagonist-induced Nur77 expression (Maheux et al., 2012), indicating that glutamate positively controls Nur77 expression in the striatum. These data show that Nur77 may play an integrative role of multiple signaling in the striatum.
mRNA levels of Nur77 and Nor-1 are extremely low or absent in adult midbrain dopaminergic neurons of substantia nigra (SN) and ventral tegmental area (VTA) in basal conditions (Zetterstrom et al., 1996a,b). However, current data indicate that Nur77 may play an important role regulating dopamine biochemical homeostasis in these nuclei. Nur77-deficient mice show an increase in dopamine D2 receptors in dorsolateral striatum; enhanced spontaneous locomotor activity; greater sensitivity to dopamine D2 receptor agonists and higher levels of DOPAC relative to the wild-type (Gilbert et al., 2006). Remarkably, Nur77 and Nor-1 expression increases significantly in midbrain dopamine neurons after the administration of dopamine D2 receptor antagonists (Maheux et al., 2005).
Unlike Nur77 and Nor-1, Nurr1 is expressed under basal physiological conditions in dopamine neurons of SN and VTA (Xiao et al., 1996; Zetterstrom et al., 1996a; Backman et al., 1999; Ojeda et al., 2003). Strong evidence indicates that Nurr1 is essential for the development of these neurons (Zetterstrom et al., 1997; Castillo et al., 1998; Saucedo-Cardenas et al., 1998). More recently, it was shown that Nurr1 is also critical to maintain midbrain dopamine neurons in the adult (Kadkhodaei et al., 2009). During development, dopamine midbrain precursors do not differentiate into dopaminergic phenotype in the Nurr1 null mice (Zetterstrom et al., 1997; Castillo et al., 1998; Saucedo-Cardenas et al., 1998). Dopaminergic markers like tyrosine hydroxylase (TH), the dopamine transporter (DAT), the vesicular monoamine transporter (VMAT2) and the tyrosine kinase receptor RET (Wallen et al., 2001) are not expressed in the ventral mesencephalon of Nurr1 null mice at birth (reviewed in Smidt et al., 2003; Perlmann and Wallen-Mackenzie, 2004). On the other hand, a progressive decline in the expression of the same genes in the SN and VTA is observed when Nurr1 is ablated in adults (Kadkhodaei et al., 2009, 2013; Galleguillos et al., 2010). Interestingly, it was recently shown that Nurr1 regulates the expression of a set of mitochondria genes (Kadkhodaei et al., 2013). Whether, the role of Nurr1 keeping dopamine neuron fully functional during adulthood and aging is related to the control of genes of the phenotype or mitochondrial genes or both requires further evidence.
The accumulated evidence indicate that Nurr1 regulates the transcription of dopamine phenotype genes (Sakurada et al., 1999; Iwawaki et al., 2000; Sacchetti et al., 2001; Hermanson et al., 2003; Kim et al., 2003; Smits et al., 2003; Galleguillos et al., 2010). Studies in Nurr1-deficient mice (±) indicate that the amount of Nurr1 is important to keep homeostasis during the life of dopamine neurons (Le et al., 1999; Jiang et al., 2005; Eells et al., 2006; Zhang et al., 2012). For instance, aged Nurr1 (±) mice have a significant decrease in rotarod performance and locomotor activity, a motor impairment analogous to Parkinson's disease associated with decreased dopamine levels in the striatum (Jiang et al., 2005). Recently, it was shown that a decreased number of TH positive cells in SN, observed in aged Nurr1 (±) mice, correlates with decreased dopamine release in the striatum (Zhang et al., 2012). Similarly, we showed that inducing a 50% decrease of Nurr1 expression in the SN of adult rats results in a significant decrease of dopamine extracellular levels in the striatum associated with decreased expression of the tyrosine kinase receptor RET (Galleguillos et al., 2010). Nurr1-deficient mice (±) also show some symptoms related to schizophrenia, such as hyperactivity in a novel environment, deficiency in the retention of emotional memory and increased response to swim stress; all symptoms associated with dysfunctions in dopamine neurotransmission (Rojas et al., 2007; Vuillermot et al., 2011). Thus, these data show that Nurr1 controls the expression of dopamine phenotype genes during adulthood. In addition, the data show that survival pathways, which are stressed during aging, require a stronger Nurr1 signaling, that cannot be achieved in Nurr1 heterozygous mice. Remarkably, the amount of Nurr1 protein levels seems to play a significant role controlling the expression of specific sets of target genes. Indeed, the group of Bannon (Johnson et al., 2011), showed that different set of genes are controlled by lower vs. higher Nurr1 level.
How Nurr1 is regulated by dopamine levels and adjusts the expression of target genes accordingly? Accumulated data indicates that Nurr1 expression depends on dopamine signaling, mainly through D2 receptors. D2 receptors are located in soma and dendrites, to regulate firing rate of neurons and presynaptically in axons to regulate dopamine synthesis and release. Drugs of abuse like cocaine decrease Nurr1 in mesencephalon (Bannon et al., 2002, 2004). D2 receptor knockout mice have increased Nurr1 expression in midbrain dopamine neurons (Tseng et al., 2000) and the loss of dopamine in rat striatum induced by 6-hydroxydopamine generates a rapid increase of Nurr1 expression in dopamine neurons of the SN (Ojeda et al., 2003). These data show that dopamine extracellular levels influence the expression of Nurr1 in the SN and VTA. Recent evidence indicates that neuronal firing regulates differentially the expression of Nurr1 and Nur77 in dopamine neurons (Eells et al., 2012). A normal flow of impulses maintains basal expression of Nurr1. Surprisingly, Nur77 was induced with a lower D2 autoreceptor activation in the VTA (Eells et al., 2012). Recently, it was shown that D2 receptors located postsynaptically in GABA projecting neurons of the striatum also regulate dopamine release (Anzalone et al., 2012). Nurr1 and/or Nur77 could be the targets of such a feedback mechanism regulating dopamine homeostasis. The recent findings of Nur77 controlling dopaminergic homeostasis raises several questions regarding the specific role of each Nur factor in dopaminergic gene expression: Are Nur factors redundant or they play particular roles in the adaptation and survival processes of midbrain dopamine neurons during adulthood and aging?
Nur Transcription Factors During the Stress Response
In response to stressful stimuli, secretory neurons of the paraventricular nucleus (PVN) discharge corticotropin-releasing factor (CRF) that in turn increases both the secretion of adrenocorticotrophin hormone (ACTH) and the transcription of its precursor, the proopiomelanocortin (POMC) gene, in the anterior pituitary. ACTH stimulates the release of glucocorticoids (GCs) and the transcription of genes encoding several steroidogenic enzymes in the adrenal gland. GCs exert diverse effects on target tissues to mobilize energy for the body to deal with the stressor, and also exert a negative feedback through the inhibition of the synthesis and secretion of CRF and POMC (Keller-Wood and Dallman, 1984; Sawchenko and Swanson, 1985; Antoni, 1986; Swanson and Simmons, 1989).
Several lines of evidence indicate that Nur transcription factors play a prominent role in adaptive responses to stress, regulating the transcription of target genes in the hypothalamus-pituitary-adrenal (HPA) axis (Figure 4). The evidence indicates that this nuclear receptor subfamily regulates the expression of CRF and POMC in the PVN and pituitary, respectively (Murphy and Conneely, 1997; Drouin et al., 1998). In the adrenal glands, Nur77 and Nor-1 also regulate the expression of steroid-21α-hydroxylase and 3-β-hydroxysteroid dehydrogenase, both enzymes essential for the production of GCs (Figure 4) (Wilson et al., 1993b; Fernandez et al., 2000; Bassett et al., 2004).
Figure 4. Scheme of Nur factors expression in the HPA axis.
A complex signaling cascade involving Nur factors is required for POMC regulation. The group of Eduardo Arzt (Kovalovsky et al., 2002) showed that in AtT-20 corticotrophs, CRF induces both Nur77 and Nurr1 expression resulting in POMC transcription through a NurRE site in its promoter. While CRF-mediated induction of Nur factors requires protein kinase A (PKA) and cAMP signaling, POMC transcriptional induction depends further on MAPK activation. Nur77-dependent induction of POMC transcription required the phosphorylation of Nur77 by MAPK (Kovalovsky et al., 2002). Concordantly, cAMP/PKA signaling enhances DNA binding activity of Nur dimers, but not monomers, and the recruitment of transcriptional coactivators through the AF-1 domain of Nur factors (Maira et al., 2003). Nur transcription factors are also involved in the ending of the stress response. The effect of Nur transcription factors on POMC promoter is antagonized by GCs (Philips et al., 1997; Drouin et al., 1998; Martens et al., 2005; Carpentier et al., 2008). The NurRE element of the POMC gene, which binds Nur dimers, is the target of the repressive effect of GCs. GC receptors bound to ligands directly interact with Nur factors inducing the transrepression of POMC (Martens et al., 2005).
Less is known about the role of Nur factors regulating CRF in the PVN. While Nurr1 is expressed constitutively in the PVN (Saucedo-Cardenas and Conneely, 1996), Nur77 is transiently induced by different types of stressful stimuli (Chan et al., 1993; Honkaniemi et al., 1994; Imaki et al., 1996; Kawasaki et al., 2005), and by central administration of CRF (Parkes et al., 1993). Interestingly, an increased expression of Nur77 occurs in the PVN of virgin and lactating females, but not in pregnant mice stressed either by exposure to a novel environment or forced swimming (Douglas et al., 2003), suggesting a role for sex hormones regulating Nur factors action. In 1997, Murphy and Conneely showed that Nurr1 and Nur77 bind and transactivate the CRF gene (Murphy and Conneely, 1997). The CRF gene promoter harbors a conserved NBRE element (Murphy et al., 1996; Yao and Denver, 2007; Yao et al., 2007), which is required for Nur77-dependent inductive effect of reporters driven by the CRF promoter (Murphy and Conneely, 1997). More recently, Stroth et al. (2011) showed that up-regulation of mRNAs encoding CRF and Nur factors in the PVN during stress depends on Pituitary Adenylate Cyclase Activating Polypeptide (PACAP). Indeed, not only CRF, but also restraint-induced expression of Nur77 and Nurr1 decreases in the pituitary gland of PACAP-deficient mice (Stroth et al., 2011), suggesting a reciprocal regulation between Nur factors and CRF. Normal function of the HPA axis in Nur77 null mutant mice indicates redundancy of Nur factors. Nurr1 mRNA increases in the adrenal gland of Nur77 null mice (Crawford et al., 1995). In addition, Nor-1 and Nur77 play similar, albeit distinct roles in the HPA axis. Similar to Nur77, Nor-1 activates the expression of the gene encoding steroid-21α-hydroxylase (Wilson et al., 1993b; Fernandez et al., 2000) through NBRE elements. In contrast, DNA binding experiments of Nor-1 to NurRE element suggest that Nor-1 is not an efficient substitute of Nur77 activation of POMC gene expression in the pituitary gland (Fernandez et al., 2000).
In addition to its crucial role in the activation of the HPA axis, CRF functions as a neurotransmitter/neuromodulator coordinating extra-hypothalamic aspects of the stress response (Aguilera, 1998; Ziegler and Herman, 2000; Herman et al., 2002). The extra-hypothalamic aspects of the stress response are mediated by interconnected nuclei of the amygdala and the bed nucleus of the stria terminalis (BNST) (Walker et al., 2003). The BNST is the primary integrative center of excitatory and inhibitory inputs regulating the HPA axis during stress (Forray and Gysling, 2004), and mediates anxiety-like behavior resulting of extended threat (Walker et al., 2009). The expression of Nur transcription factors increases in the limbic nuclei associated to the extra-hypothalamic aspects of the stress response, suggesting that Nur factors could also underlie enduring changes induced by chronic stress. For instance, Nor-1 is increased in the somatosensory cortex and amygdala when exposed to novelty stress (Sun et al., 2007). Predator stress increases Nur77 mRNA in prelimbic, infralimbic and, ventral and lateral orbital prefrontal cortexes (Schiltz et al., 2007). Nurr1 expression increases while TH decreases at postnatal day 7 in the VTA of prenatally stressed offspring, which suggest a possible compensatory mechanism that may play Nurr1 to counteract the observed reduction of dopamine levels (Katunar et al., 2009, 2010). In addition, dopamine content decreases within the PFC and midbrain of rats subjected to forced swim test, meanwhile Nurr1 expression increases in the same brain areas (Rojas et al., 2010) supporting a role for Nurr1 counteracting the decrease of dopamine content. Intriguingly, Eells et al. (2002) showed that Nurr1 (±) mice displayed significantly greater locomotor activity in response to mild stress that correlated with lower dopamine content in mesolimbic and mesocortical circuits (Eells et al., 2002). Taken together the data indicate that Nur factors expression is modulated in limbic circuit during the response to stress. It is unknown what are the genes induced or repressed by Nur factors in the nuclei associated to the extra-hypothalamic aspects of the stress response. One possible target is CRF.
Repeated immobilization stress and chronic mild stress induce CRF expression in the BNST (Stout et al., 2000; Santibanez et al., 2006). We reported that cells expressing CRF in the BNST also express Nur77 (Campos-Melo et al., 2011), even though the expression of Nur77 is wider than CRF in this nucleus. Nur77 expression increases significantly in the dorso-lateral and ventro-medial subdivisions of the BNST after acute and repeated immobilization stress (Campos-Melo et al., 2011), same areas where CRF expression increases after repeated immobilization stress (Santibanez et al., 2006). Several years ago, it was shown that the intracerebroventricular injection of CRF increases CRF expression in the PVN (Parkes et al., 1993). A CRF-dependent induction of CRF could be a mechanism of maintaining CRF expression in brain nuclei associated to chronic stress-induced anxiety and depression (Stenzel-Poore et al., 1994; Pelton et al., 1997; Yao and Denver, 2007). In support of this suggestion (Parkes et al., 1993), immunohistochemical data show that CRF neurons are innervated by CRF axons in PVN and amygdala (Moga et al., 1989; Silverman et al., 1989; Moga and Saper, 1994). Similarly, in BNST, positive CRF terminals also innervate CRF neurons (Sakanaka et al., 1986; Veinante et al., 1997). Since CRF induces the expression of Nur77 (Kovalovsky et al., 2002) and Nur77 also is able to induce CRF expression (Murphy and Conneely, 1997), it is possible that Nur77 could mediate the vicious cycle of CRF-dependent CRF induction in the PVN and limbic nuclei, during chronic stress; an hypothesis that requires further investigation.
Functional Role of Nur Transcription Factors in the Addiction Process. Protectors or Instigators?
The motivation/reward circuit has its roots in dopamine neurons located in the VTA, which send afferences to the NAc, BNST, septum, amygdala, and PFC. Acute administration of drugs of abuse, which increase dopamine release (Di Chiara and Imperato, 1988), induce the expression of Nur77 and Nor-1 in nuclei of the motivation/reward circuit (Table 1) (Werme et al., 2000a; St-Hilaire et al., 2003b; Krasnova et al., 2011). Nur77 and Nor-1 are up-regulated in the NAc, striatum, and cortex after acute administration of cocaine and morphine (Werme et al., 2000a). Similarly, acute administration of methamphetamine up-regulates the expression of Nur77 in cortex, striatum and NAc core; and of Nurr1 in the cortex and VTA. Pretreatment with a selective antagonist of D1/D5 dopamine receptors prevents methamphetamine-induced expression of both Nur77 and Nurr1 mRNA, supporting that dopamine-mediated signaling regulates Nur transcription factors expression (Akiyama et al., 2008). In concordance, the simultaneous administration of D1 and D2 agonists increases Nur77 expression in the striatum (St-Hilaire et al., 2003b). However, D1 agonists administered alone do not modify Nur77 expression in the striatum (St-Hilaire et al., 2003a). As analyzed before, substantial data indicate that dopamine D2 antagonists increase, while D2 agonists decrease Nur77 and Nor-1 expression in the striatum (Beaudry et al., 2000; Werme et al., 2000a,b; Langlois et al., 2001; Maheux et al., 2005, 2012; St-Hilaire et al., 2005). How could this paradox be explained? Taken together the available evidence suggests that drugs of abuse would require another neurotransmitter system, besides dopamine, in order to induce Nur77 in GABA projecting neurons of the striatum. Nur77 induction by D2 antagonists in GABA projecting neurons depends on glutamate signaling through mGlu5 receptors (Maheux et al., 2012). Maheux et al. (2012) showed that ablation of the long isoform of dopamine D2 receptors, located post-synaptically do not prevent D2 antagonists-dependent induction of Nur77, indicating that the effect is presynaptic, where the short isoform of the D2 receptor is present. Presynaptic D2 receptors are located in dopaminergic and glutamatergic axons in the striatum. This result was further supported by showing that interrupting glutamate neurotransmission to the striatum by cortex lesion prevented the increase of Nur77 expression induced by D2 antagonists (Maheux et al., 2012). It is tempting to suggest that similarly, drugs of abuse-dependent induction of Nur77 in the striatum and NAc depends, besides dopamine, on glutamate neurotransmission. Dopamine- and glutamate-dependent induction of Nur77 supports a role for Nur77 integrating pre and postsynaptic information in striatal GABA projecting neurons.
The development of compulsive running, associated with a high risk of addictive behavior, correlates with lower Nur77 and Nor1 expression in several nuclei of the motivation/reward circuit, in the addiction-prone Lewis rat strain compared with the less-addiction prone Fisher rats which do not develop compulsive running (Werme et al., 1999). Accordingly, Nur77 null mice show increased locomotor activity, but a similar locomotor sensitization than wild type mice after repeated amphetamine administration (Bourhis et al., 2009). Interestingly, the blockade of amphetamine-induced locomotor sensitization by an RXR antagonist is abolished in Nur77 null mice (Bourhis et al., 2009). Together the data suggest that Nur77 regulates addiction-prone phenotype and sensitization by different mechanisms. The available information regarding Nurr1 protecting or facilitating addictive behaviors is unclear. In one study it was shown that Nurr1 (±) mice do not develop compulsive running behavior and high ethanol consumption compared to wild type mice (Werme et al., 2003). In another study it was shown that Nurr1 (±) mice have an increased basal locomotor activity and augmented locomotor response to acute methamphetamine administration (Backman et al., 2003). Mice genetic background, behavioral protocols, among other parameters, may influence these observations. Remarkably, in a recent work it was shown that ablation of the histone deacetylase HDAC3 in the NAc facilitates condition-place preference induced by cocaine (Rogge et al., 2013). This effect was correlated with increased Nurr1 expression in this nucleus, supporting a role for Nurr1 facilitating addiction behaviors (Rogge et al., 2013).
It has been proposed that the persistent behavioral and cognitive effects of chronic intake of drugs of abuse depend on new programs of gene expression triggered by immediate-early genes. Tolerance and sensitization of Nur factors expression in nuclei of the motivation/reward and stress brain circuits suggest that these early genes play a signaling role in the plastic changes underlying long-term adaptations. Studies in cocaine abusers showed a reduction of Nurr1 expression in SN neurons (Nielsen et al., 2008). This decreased expression of Nurr1 correlates with a reduction of DAT expression in the same neurons (Bannon et al., 2002). Similarly, rats chronically treated with cocaine show a down-regulation of the expression of Nurr1 mRNA and protein in the ventral midbrain (Leo et al., 2007). Additionally, chronic use of heroin decreases Nurr1 mRNA to a greater extent with age in the paranigral nucleus of the VTA (Horvath et al., 2007). Nurr1 expression decreases after chronic intake of drugs of abuse, could be an adaptive change to excessive dopamine stimulation, and also could be indicative of Nurr1 role adjusting the expression of dopamine target genes during the addiction process. In this regard, it was shown that an acute methamphetamine challenge to animals pretreated with methamphetamine causes a further decrease in Nurr1 mRNA levels (McCoy et al., 2011), indicating that the signaling system regulating Nurr1 expression adapts to new parameters.
Opposing to a tolerance effect observed for Nurr1; Nur77 seems to adapt to dopamine changes and its levels are still inducible after chronic drug intake. For instance, it has been shown that Nur77 expression increases in the frontal cortex of rats after 10 days of cocaine self-administration (Freeman et al., 2002b) or 14 days in a binge model of cocaine administration (Freeman et al., 2002a), and in the dorsal striatum after 7 days of cocaine self-administration (Lynch et al., 2008). On other hand, it was shown that the expression of several early-genes, including Nur factors, is no longer induced in the striatum after chronic exposure to methamphetamine (McCoy et al., 2011). It is noteworthy that Nor-1 is still significantly induced in the striatum of methamphetamine chronically-treated rats, indicating the capacity of the system to respond in new settings.
Chronic stress also triggers tolerance and sensitization of Nur factors expression. In the PVN, it has been shown that Nur77 is no longer induced after chronic stress stimuli (Umemoto et al., 1994, 1997). In contrast, in the ventral region of the BNST, we observed a higher number of cells expressing Nur77 after repeated immobilization stress compared with acute stress (Campos-Melo et al., 2011). Interestingly, the data of the group of Marta Antonelli (Katunar et al., 2010) suggest that Nurr1 may be the transcription factor setting up the new parameters of the dopamine system after prenatal stress. Using prenatal restraint stress, they showed that Nurr1 is permanently higher in the VTA, but not in the SN, in the offspring of stressed mothers. This increment of Nurr1 expression in the VTA correlates with several changes in the motivation/reward dopamine system that persist to adulthood (Baier et al., 2012). An exciting work indicates that Nur factors integrate stress and drug addiction signaling. Postweaning isolation causes elevation in amphetamine-induced dopamine overflow in Nurr1 (−/+) mice, but a reduction in (+/+) mice (Moore et al., 2008). These data demonstrate that a deletion of a single allele of Nurr1, which produced only subtle phenotypic changes, when coupled with a developmental stressor such as postweaning isolation, can dramatically alter mesoaccumbens dopamine neurotransmission (Moore et al., 2008).
Conclusions
Chronic stress plays a primary role in the origin of several brain pathologies such as anxiety and depression (McCormick and Green, 2013), and facilitates and perpetuates drug addiction (Koob, 2008; Sinha, 2008). Chronic stress and repeated use of addictive drugs induce long-lasting alterations of the motivation/reward circuit and HPA axis (Koob and Le Moal, 2001). The evidence presented points to Nur transcription factors as orchestrators of the molecular bases of the reorganization of these circuits under stressful stimuli and exposure to addictive drugs, since their expression is fast, transient and strongly regulated by dopamine, glutamate, and CRF in the nuclei of the motivation/reward circuit and HPA axis. The features of Nur factors as early genes and orphan nuclear receptors allow them to integrate and transmit fast responses to incoming neurotransmitter signals in neurons. The transient nature of the changes in Nur factor levels suggest that they can re-program the expression of target genes in response to acute and chronic dopamine changes by adjusting their inducibility to the new conditions, as occurring during chronic stress or after repeated exposure to drugs of abuse. Finally, the localization of Nurr1 in dopamine neurons and Nur77/Nor-1 in dopamine-receptive neurons, positions them to translate the dopaminergic information simultaneously to the genome of pre and post-synaptic neurons, allowing an integrative signaling of the motivation/reward circuit. Identifying the intracellular signaling pathways inducing Nur factors expression and their target genes is essential to elucidate their function in normal physiology as well as in addiction and anxiety disorders. These findings might offer novel targets to treat these devastating conditions.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Acknowledgments
Our work cited in this manuscript was supported by FONDECYT grant N° 3085027 to Danae Campos-Melo, and FONDECYT grants N° 1070349 and 1110352 to María E. Andrés. The Millennium Nucleus in Stress and Addiction is supported by MSI grant N° P10/063-F.
References
Aguilera, G. (1998). Corticotropin releasing hormone, receptor regulation and the stress response. Trends Endocrinol. Metab. 9, 329–336. doi: 10.1016/S1043-2760(98)00079-4
Akiyama, K., Isao, T., Ide, S., Ishikawa, M., and Saito, A. (2008). mRNA expression of the Nurr1 and NGFI-B nuclear receptor families following acute and chronic administration of methamphetamine. Prog. Neuropsychopharmacol. Biol. Psychiatry 32, 1957–1966. doi: 10.1016/j.pnpbp.2008.09.021
Alvarez-Castelao, B., Losada, F., Ahicart, P., and Castano, J. G. (2013). The N-terminal region of Nurr1 (a.a 1-31) is essential for its efficient degradation by the ubiquitin proteasome pathway. PLoS ONE 8:e55999. doi: 10.1371/journal.pone.0055999
Antoni, F. A. (1986). Hypothalamic control of adrenocorticotropin secretion: advances since the discovery of 41-residue corticotropin-releasing factor. Endocr. Rev. 7, 351–378. doi: 10.1210/edrv-7-4-351
Anzalone, A., Lizardi-Ortiz, J. E., Ramos, M., De Mei, C., Hopf, F. W., Iaccarino, C., et al. (2012). Dual control of dopamine synthesis and release by presynaptic and postsynaptic dopamine D2 receptors. J. Neurosci. 32, 9023–9034. doi: 10.1523/JNEUROSCI.0918-12.2012
Arredondo, C., Orellana, M., Vecchiola, A., Pereira, L. A., Galdames, L., and Andres, M. E. (2013). PIASgamma enhanced SUMO-2 modification of Nurr1 activation-function-1 domain limits Nurr1 transcriptional synergy. PLoS ONE 8:e55035. doi: 10.1371/journal.pone.0055035
Backman, C., Perlmann, T., Wallen, A., Hoffer, B. J., and Morales, M. (1999). A selective group of dopaminergic neurons express Nurr1 in the adult mouse brain. Brain Res. 851, 125–132. doi: 10.1016/S0006-8993(99)02149-6
Backman, C., You, Z. B., Perlmann, T., and Hoffer, B. J. (2003). Elevated locomotor activity without altered striatal dopamine contents in Nurr1 heterozygous mice after acute exposure to methamphetamine. Behav. Brain Res. 143, 95–100. doi: 10.1016/S0166-4328(03)00029-9
Baier, C. J., Katunar, M. R., Adrover, E., Pallares, M. E., and Antonelli, M. C. (2012). Gestational restraint stress and the developing dopaminergic system: an overview. Neurotox. Res. 22, 16–32. doi: 10.1007/s12640-011-9305-4
Bannon, M. J., Pruetz, B., Barfield, E., and Schmidt, C. J. (2004). Transcription factors specifying dopamine phenotype are decreased in cocaine users. Neuroreport 15, 401–404. doi: 10.1097/00001756-200403010-00003
Bannon, M. J., Pruetz, B., Manning-Bog, A. B., Whitty, C. J., Michelhaugh, S. K., Sacchetti, P., et al. (2002). Decreased expression of the transcription factor NURR1 in dopamine neurons of cocaine abusers. Proc. Natl. Acad. Sci. U.S.A. 99, 6382–6385. doi: 10.1073/pnas.092654299
Bassett, M. H., Suzuki, T., Sasano, H., De Vries, C. J., Jimenez, P. T., Carr, B. R., et al. (2004). The orphan nuclear receptor NGFIB regulates transcription of 3beta-hydroxysteroid dehydrogenase. implications for the control of adrenal functional zonation. J. Biol. Chem. 279, 37622–37630. doi: 10.1074/jbc.M405431200
Beaudry, G., Langlois, M. C., Weppe, I., Rouillard, C., and Levesque, D. (2000). Contrasting patterns and cellular specificity of transcriptional regulation of the nuclear receptor nerve growth factor-inducible B by haloperidol and clozapine in the rat forebrain. J. Neurochem. 75, 1694–1702. doi: 10.1046/j.1471-4159.2000.0751694.x
Benoit, G., Malewicz, M., and Perlmann, T. (2004). Digging deep into the pockets of orphan nuclear receptors: insights from structural studies. Trends Cell Biol. 14, 369–376. doi: 10.1016/j.tcb.2004.05.007
Boudreaux, S. P., Ramirez-Herrick, A. M., Duren, R. P., and Conneely, O. M. (2012). Genome-wide profiling reveals transcriptional repression of MYC as a core component of NR4A tumor suppression in acute myeloid leukemia. Oncogenesis 1, e19. doi: 10.1038/oncsis.2012.19
Bourhis, E., Maheux, J., Paquet, B., Kagechika, H., Shudo, K., Rompre, P. P., et al. (2009). The transcription factors Nur77 and retinoid X receptors participate in amphetamine-induced locomotor activities. Psychopharmacology (Berl.) 202, 635–648. doi: 10.1007/s00213-008-1343-0
Brosenitsch, T. A., and Katz, D. M. (2001). Physiological patterns of electrical stimulation can induce neuronal gene expression by activating N-type calcium channels. J. Neurosci. 21, 2571–2579.
Campos-Melo, D., Quiroz, G., Noches, V., Gysling, K., Forray, M. I., and Andres, M. E. (2011). Repeated immobilization stress increases nur77 expression in the bed nucleus of the stria terminalis. Neurotox. Res. 20, 289–300. doi: 10.1007/s12640-011-9243-1
Carpentier, R., Sacchetti, P., Segard, P., Staels, B., and Lefebvre, P. (2008). The glucocorticoid receptor is a co-regulator of the orphan nuclear receptor Nurr1. J. Neurochem. 104, 777–789. doi: 10.1111/j.1471-4159.2007.05055.x
Castillo, S. O., Baffi, J. S., Palkovits, M., Goldstein, D. S., Kopin, I. J., Witta, J., et al. (1998). Dopamine biosynthesis is selectively abolished in substantia nigra/ventral tegmental area but not in hypothalamic neurons in mice with targeted disruption of the Nurr1 gene. Mol. Cell. Neurosci. 11, 36–46. doi: 10.1006/mcne.1998.0673
Chan, R. K., Brown, E. R., Ericsson, A., Kovacs, K. J., and Sawchenko, P. E. (1993). A comparison of two immediate-early genes, c-fos and NGFI-B, as markers for functional activation in stress-related neuroendocrine circuitry. J. Neurosci. 13, 5126–5138.
Chergui, K., Svenningsson, P., Nomikos, G. G., Gonon, F., Fredholm, B. B., and Svennson, T. H. (1997). Increased expression of NGFI-A mRNA in the rat striatum following burst stimulation of the medial forebrain bundle. Eur. J. Neurosci. 9, 2370–2382. doi: 10.1111/j.1460-9568.1997.tb01654.x
Crawford, P. A., Sadovsky, Y., Woodson, K., Lee, S. L., and Milbrandt, J. (1995). Adrenocortical function and regulation of the steroid 21-hydroxylase gene in NGFI-B-deficient mice. Mol. Cell. Biol. 15, 4331.
Davis, M. I., and Puhl, H. L. 3rd. (2011). Nr4a1-eGFP is a marker of striosome-matrix architecture, development and activity in the extended striatum. PLoS ONE 6:e16619. doi: 10.1371/journal.pone.0016619
Deyoung, R. A., Baker, J. C., Cado, D., and Winoto, A. (2003). The orphan steroid receptor Nur77 family member Nor-1 is essential for early mouse embryogenesis. J. Biol. Chem. 278, 47104–47109. doi: 10.1074/jbc.M307496200
Di Chiara, G., and Imperato, A. (1988). Drugs abused by humans preferentially increase synaptic dopamine concentrations in the mesolimbic system of freely moving rats. Proc. Natl. Acad. Sci. U.S.A. 85, 5274–5278. doi: 10.1073/pnas.85.14.5274
Douglas, A. J., Brunton, P. J., Bosch, O. J., Russell, J. A., and Neumann, I. D. (2003). Neuroendocrine responses to stress in mice: hyporesponsiveness in pregnancy and parturition. Endocrinology 144, 5268–5276. doi: 10.1210/en.2003-0461
Drouin, J., Maira, M., and Philips, A. (1998). Novel mechanism of action for Nur77 and antagonism by glucocorticoids: a convergent mechanism for CRH activation and glucocorticoid repression of POMC gene transcription. J. Steroid Biochem. Mol. Biol. 65, 59–63. doi: 10.1016/S0960-0760(97)00180-5
Eells, J. B., Lipska, B. K., Yeung, S. K., Misler, J. A., and Nikodem, V. M. (2002). Nurr1-null heterozygous mice have reduced mesolimbic and mesocortical dopamine levels and increased stress-induced locomotor activity. Behav. Brain Res. 136, 267–275. doi: 10.1016/S0166-4328(02)00185-7
Eells, J. B., Misler, J. A., and Nikodem, V. M. (2006). Reduced tyrosine hydroxylase and GTP cyclohydrolase mRNA expression, tyrosine hydroxylase activity, and associated neurochemical alterations in Nurr1-null heterozygous mice. Brain Res. Bull. 70, 186–195. doi: 10.1016/j.brainresbull.2006.05.004
Eells, J. B., Wilcots, J., Sisk, S., and Guo-Ross, S. X. (2012). NR4A gene expression is dynamically regulated in the ventral tegmental area dopamine neurons and is related to expression of dopamine neurotransmission genes. J. Mol. Neurosci. 46, 545–553. doi: 10.1007/s12031-011-9642-z
Ethier, I., Beaudry, G., St-Hilaire, M., Milbrandt, J., Rouillard, C., and Levesque, D. (2004). The transcription factor NGFI-B (Nur77) and retinoids play a critical role in acute neuroleptic-induced extrapyramidal effect and striatal neuropeptide gene expression. Neuropsychopharmacology 29, 335–346. doi: 10.1038/sj.npp.1300318
Fernandez, P. M., Brunel, F., Jimenez, M. A., Saez, J. M., Cereghini, S., and Zakin, M. M. (2000). Nuclear receptors Nor1 and NGFI-B/Nur77 play similar, albeit distinct, roles in the hypothalamo-pituitary-adrenal axis. Endocrinology 141, 2392–2400. doi: 10.1210/en.141.7.2392
Flaig, R., Greschik, H., Peluso-Iltis, C., and Moras, D. (2005). Structural basis for the cell-specific activities of the NGFI-B and the Nurr1 ligand-binding domain. J. Biol. Chem. 280, 19250–19258. doi: 10.1074/jbc.M413175200
Forray, M. I., and Gysling, K. (2004). Role of noradrenergic projections to the bed nucleus of the stria terminalis in the regulation of the hypothalamic-pituitary-adrenal axis. Brain Res. Brain Res. Rev. 47, 145–160. doi: 10.1016/j.brainresrev.2004.07.011
Freeman, W. M., Brebner, K., Lynch, W. J., Patel, K. M., Robertson, D. J., Roberts, D. C., et al. (2002a). Changes in rat frontal cortex gene expression following chronic cocaine. Brain Res. Mol. Brain Res. 104, 11–20. doi: 10.1016/S0169-328X(02)00197-3
Freeman, W. M., Brebner, K., Patel, K. M., Lynch, W. J., Roberts, D. C., and Vrana, K. E. (2002b). Repeated cocaine self-administration causes multiple changes in rat frontal cortex gene expression. Neurochem. Res. 27, 1181–1192. doi: 10.1023/A:1020929526688
Galleguillos, D., Fuentealba, J. A., Gomez, L. M., Saver, M., Gomez, A., Nash, K., et al. (2010). Nurr1 regulates RET expression in dopamine neurons of adult rat midbrain. J. Neurochem. 114, 1158–1167. doi: 10.1111/j.1471-4159.2010.06841.x
Galleguillos, D., Vecchiola, A., Fuentealba, J. A., Ojeda, V., Alvarez, K., Gomez, A., et al. (2004). PIASgamma represses the transcriptional activation induced by the nuclear receptor Nurr1. J. Biol. Chem. 279, 2005–2011. doi: 10.1074/jbc.M308113200
Gervais, J., Soghomonian, J. J., Richard, D., and Rouillard, C. (1999). Dopamine and serotonin interactions in the modulation of the expression of the immediate-early transcription factor, nerve growth factor-inducible B, in the striatum. Neuroscience 91, 1045–1054. doi: 10.1016/S0306-4522(98)00688-5
Giguere, V. (1999). Orphan nuclear receptors: from gene to function. Endocr. Rev. 20, 689–725. doi: 10.1210/er.20.5.689
Gilbert, F., Morissette, M., St-Hilaire, M., Paquet, B., Rouillard, C., Di Paolo, T., et al. (2006). Nur77 gene knockout alters dopamine neuron biochemical activity and dopamine turnover. Biol. Psychiatry 60, 538–547. doi: 10.1016/j.biopsych.2006.04.023
Gofflot, F., Chartoire, N., Vasseur, L., Heikkinen, S., Dembele, D., Le Merrer, J., et al. (2007). Systematic gene expression mapping clusters nuclear receptors according to their function in the brain. Cell 131, 405–418. doi: 10.1016/j.cell.2007.09.012
Hazel, T. G., Nathans, D., and Lau, L. F. (1988). A gene inducible by serum growth factors encodes a member of the steroid and thyroid hormone receptor superfamily. Proc. Natl. Acad. Sci. U.S.A. 85, 8444–8448. doi: 10.1073/pnas.85.22.8444
Herman, J. P., Cullinan, W. E., Ziegler, D. R., and Tasker, J. G. (2002). Role of the paraventricular nucleus microenvironment in stress integration. Eur. J. Neurosci. 16, 381–385. doi: 10.1046/j.1460-9568.2002.02133.x
Hermanson, E., Joseph, B., Castro, D., Lindqvist, E., Aarnisalo, P., Wallen, A., et al. (2003). Nurr1 regulates dopamine synthesis and storage in MN9D dopamine cells. Exp. Cell Res. 288, 324–334. doi: 10.1016/S0014-4827(03)00216-7
Honkaniemi, J., Kononen, J., Kainu, T., Pyykonen, I., and Pelto-Huikko, M. (1994). Induction of multiple immediate early genes in rat hypothalamic paraventricular nucleus after stress. Brain Res. Mol. Brain Res. 25, 234–241. doi: 10.1016/0169-328X(94)90158-9
Honkaniemi, J., and Sharp, F. R. (1999). Prolonged expression of zinc finger immediate-early gene mRNAs and decreased protein synthesis following kainic acid induced seizures. Eur. J. Neurosci. 11, 10–17. doi: 10.1046/j.1460-9568.1999.00401.x
Horvath, M. C., Kovacs, G. G., Kovari, V., Majtenyi, K., Hurd, Y. L., and Keller, E. (2007). Heroin abuse is characterized by discrete mesolimbic dopamine and opioid abnormalities and exaggerated nuclear receptor-related 1 transcriptional decline with age. J. Neurosci. 27, 13371–13375. doi: 10.1523/JNEUROSCI.2398-07.2007
Imaki, T., Shibasaki, T., Chikada, N., Harada, S., Naruse, M., and Demura, H. (1996). Different expression of immediate-early genes in the rat paraventricular nucleus induced by stress: relation to corticotropin-releasing factor gene transcription. Endocr. J. 43, 629–638. doi: 10.1507/endocrj.43.629
Iwawaki, T., Kohno, K., and Kobayashi, K. (2000). Identification of a potential nurr1 response element that activates the tyrosine hydroxylase gene promoter in cultured cells. Biochem. Biophys. Res. Commun. 274, 590–595. doi: 10.1006/bbrc.2000.3204
Jacobs, F. M., van Erp, S., van der Linden, A. J., von Oerthel, L., Burbach, J. P., and Smidt, M. P. (2009). Pitx3 potentiates Nurr1 in dopamine neuron terminal differentiation through release of SMRT-mediated repression. Development 136, 531–540. doi: 10.1242/dev.029769
Jacobs, O., Van Bree, L., Mailleux, P., Zhang, F., Schiffmann, S. N., Halleux, P., et al. (1994). Homolateral cerebrocortical increase of immediate early gene and neurotransmitter messenger RNAs after minimal cortical lesion: blockade by N-methyl-D-aspartate antagonist. Neuroscience 59, 827–836. doi: 10.1016/0306-4522(94)90287-9
Jiang, C., Wan, X., He, Y., Pan, T., Jankovic, J., and Le, W. (2005). Age-dependent dopaminergic dysfunction in Nurr1 knockout mice. Exp. Neurol. 191, 154–162. doi: 10.1016/j.expneurol.2004.08.035
Johnson, M. M., Michelhaugh, S. K., Bouhamdan, M., Schmidt, C. J., and Bannon, M. J. (2011). The transcription factor NURR1 exerts concentration-dependent effects on target genes mediating distinct biological processes. Front. Neurosci. 5:135. doi: 10.3389/fnins.2011.00135
Kadkhodaei, B., Alvarsson, A., Schintu, N., Ramskold, D., Volakakis, N., Joodmardi, E., et al. (2013). Transcription factor Nurr1 maintains fiber integrity and nuclear-encoded mitochondrial gene expression in dopamine neurons. Proc. Natl. Acad. Sci. U.S.A. 110, 2360–2365. doi: 10.1073/pnas.1221077110
Kadkhodaei, B., Ito, T., Joodmardi, E., Mattsson, B., Rouillard, C., Carta, M., et al. (2009). Nurr1 is required for maintenance of maturing and adult midbrain dopamine neurons. J. Neurosci. 29, 15923–15932. doi: 10.1523/JNEUROSCI.3910-09.2009
Kang, S. A., Na, H., Kang, H. J., Kim, S. H., Lee, M. H., and Lee, M. O. (2010). Regulation of Nur77 protein turnover through acetylation and deacetylation induced by p300 and HDAC1. Biochem. Pharmacol. 80, 867–873. doi: 10.1016/j.bcp.2010.04.026
Katagiri, Y., Takeda, K., Yu, Z. X., Ferrans, V. J., Ozato, K., and Guroff, G. (2000). Modulation of retinoid signalling through NGF-induced nuclear export of NGFI-B. Nat. Cell Biol. 2, 435–440. doi: 10.1038/35017072
Katunar, M. R., Saez, T., Brusco, A., and Antonelli, M. C. (2009). Immunocytochemical expression of dopamine-related transcription factors Pitx3 and Nurr1 in prenatally stressed adult rats. J. Neurosci. Res. 87, 1014–1022. doi: 10.1002/jnr.21911
Katunar, M. R., Saez, T., Brusco, A., and Antonelli, M. C. (2010). Ontogenetic expression of dopamine-related transcription factors and tyrosine hydroxylase in prenatally stressed rats. Neurotox. Res. 18, 69–81. doi: 10.1007/s12640-009-9132-z
Kawasaki, M., Yamaguchi, K., Saito, J., Ozaki, Y., Mera, T., Hashimoto, H., et al. (2005). Expression of immediate early genes and vasopressin heteronuclear RNA in the paraventricular and supraoptic nuclei of rats after acute osmotic stimulus. J. Neuroendocrinol. 17, 227–237. doi: 10.1111/j.1365-2826.2005.01297.x
Keller-Wood, M. E., and Dallman, M. F. (1984). Corticosteroid inhibition of ACTH secretion. Endocr. Rev. 5, 1–24. doi: 10.1210/edrv-5-1-1
Kim, K. S., Kim, C. H., Hwang, D. Y., Seo, H., Chung, S., Hong, S. J., et al. (2003). Orphan nuclear receptor Nurr1 directly transactivates the promoter activity of the tyrosine hydroxylase gene in a cell-specific manner. J. Neurochem. 85, 622–634. doi: 10.1046/j.1471-4159.2003.01671.x
Koob, G. F. (2008). A role for brain stress systems in addiction. Neuron 59, 11–34. doi: 10.1016/j.neuron.2008.06.012
Koob, G. F., and Le Moal, M. (2001). Drug addiction, dysregulation of reward, and allostasis. Neuropsychopharmacology 24, 97–129. doi: 10.1016/S0893-133X(00)00195-0
Kovalovsky, D., Refojo, D., Liberman, A. C., Hochbaum, D., Pereda, M. P., Coso, O. A., et al. (2002). Activation and induction of NUR77/NURR1 in corticotrophs by CRH/cAMP: involvement of calcium, protein kinase A, and MAPK pathways. Mol. Endocrinol. 16, 1638–1651. doi: 10.1210/me.16.7.1638
Krasnova, I. N., Ladenheim, B., Hodges, A. B., Volkow, N. D., and Cadet, J. L. (2011). Chronic methamphetamine administration causes differential regulation of transcription factors in the rat midbrain. PLoS ONE 6:e19179. doi: 10.1371/journal.pone.0019179
Lammi, J., Perlmann, T., and Aarnisalo, P. (2008). Corepressor interaction differentiates the permissive and non-permissive retinoid X receptor heterodimers. Arch. Biochem. Biophys. 472, 105–114. doi: 10.1016/j.abb.2008.02.003
Langlois, M. C., Beaudry, G., Zekki, H., Rouillard, C., and Levesque, D. (2001). Impact of antipsychotic drug administration on the expression of nuclear receptors in the neocortex and striatum of the rat brain. Neuroscience 106, 117–128. doi: 10.1016/S0306-4522(01)00248-2
Law, S. W., Conneely, O. M., Demayo, F. J., and O'Malley, B. W. (1992). Identification of a new brain-specific transcription factor, NURR1. Mol. Endocrinol. 6, 2129–2135. doi: 10.1210/me.6.12.2129
Le, W., Conneely, O. M., He, Y., Jankovic, J., and Appel, S. H. (1999). Reduced Nurr1 expression increases the vulnerability of mesencephalic dopamine neurons to MPTP-induced injury. J. Neurochem. 73, 2218–2221. doi: 10.1046/j.1471-4159.1999.02218.x
Lee, S. O., Li, X., Khan, S., and Safe, S. (2011). Targeting NR4A1 (TR3) in cancer cells and tumors. Expert Opin. Ther. Targets 15, 195–206. doi: 10.1517/14728222.2011.547481
Leo, D., di Porzio, U., Racagni, G., Riva, M. A., Fumagalli, F., and Perrone-Capano, C. (2007). Chronic cocaine administration modulates the expression of transcription factors involved in midbrain dopaminergic neuron function. Exp. Neurol. 203, 472–480. doi: 10.1016/j.expneurol.2006.08.024
Levesque, D., and Rouillard, C. (2007). Nur77 and retinoid X receptors: crucial factors in dopamine-related neuroadaptation. Trends Neurosci. 30, 22–30. doi: 10.1016/j.tins.2006.11.006
Li, H., Kolluri, S. K., Gu, J., Dawson, M. I., Cao, X., Hobbs, P. D., et al. (2000). Cytochrome c release and apoptosis induced by mitochondrial targeting of nuclear orphan receptor TR3. Science 289, 1159–1164. doi: 10.1126/science.289.5482.1159
Lynch, W. J., Girgenti, M. J., Breslin, F. J., Newton, S. S., and Taylor, J. R. (2008). Gene profiling the response to repeated cocaine self-administration in dorsal striatum: a focus on circadian genes. Brain Res. 1213, 166–177. doi: 10.1016/j.brainres.2008.02.106
Maheux, J., Ethier, I., Rouillard, C., and Levesque, D. (2005). Induction patterns of transcription factors of the nur family (nurr1, nur77, and nor-1) by typical and atypical antipsychotics in the mouse brain: implication for their mechanism of action. J. Pharmacol. Exp. Ther. 313, 460–473. doi: 10.1124/jpet.104.080184
Maheux, J., St-Hilaire, M., Voyer, D., Tirotta, E., Borrelli, E., Rouillard, C., et al. (2012). Dopamine D(2) antagonist-induced striatal Nur77 expression requires activation of mGlu5 receptors by cortical afferents. Front. Pharmacol. 3:153. doi: 10.3389/fphar.2012.00153
Mahmoudi, S., Blanchet, P. J., and Levesque, D. (2013). Haloperidol-induced striatal Nur77 expression in a non-human primate model of tardive dyskinesia. Eur. J. Neurosci. 38, 2192–2198. doi: 10.1111/ejn.12198
Mahmoudi, S., Samadi, P., Gilbert, F., Ouattara, B., Morissette, M., Gregoire, L., et al. (2009). Nur77 mRNA levels and L-Dopa-induced dyskinesias in MPTP monkeys treated with docosahexaenoic acid. Neurobiol. Dis. 36, 213–222. doi: 10.1016/j.nbd.2009.07.017
Maira, M., Martens, C., Batsche, E., Gauthier, Y., and Drouin, J. (2003). Dimer-specific potentiation of NGFI-B (Nur77) transcriptional activity by the protein kinase A pathway and AF-1-dependent coactivator recruitment. Mol. Cell. Biol. 23, 763–776. doi: 10.1128/MCB.23.3.763-776.2003
Maira, M., Martens, C., Philips, A., and Drouin, J. (1999). Heterodimerization between members of the Nur subfamily of orphan nuclear receptors as a novel mechanism for gene activation. Mol. Cell. Biol. 19, 7549–7557.
Martens, C., Bilodeau, S., Maira, M., Gauthier, Y., and Drouin, J. (2005). Protein-protein interactions and transcriptional antagonism between the subfamily of NGFI-B/Nur77 orphan nuclear receptors and glucocorticoid receptor. Mol. Endocrinol. 19, 885–897. doi: 10.1210/me.2004-0333
Maruyama, K., Tsukada, T., Bandoh, S., Sasaki, K., Ohkura, N., and Yamaguchi, K. (1995). Expression of NOR-1 and its closely related members of the steroid/thyroid hormone receptor superfamily in human neuroblastoma cell lines. Cancer Lett. 96, 117–122. doi: 10.1016/0304-3835(95)03921-I
Maxwell, M. A., and Muscat, G. E. (2006). The NR4A subgroup: immediate early response genes with pleiotropic physiological roles. Nucl. Recept. Signal. 4, e002. doi: 10.1621/nrs.04002
McCormick, C. M., and Green, M. R. (2013). From the stressed adolescent to the anxious and depressed adult: investigations in rodent models. Neuroscience 249, 242–257. doi: 10.1016/j.neuroscience.2012.08.063
McCoy, M. T., Jayanthi, S., Wulu, J. A., Beauvais, G., Ladenheim, B., Martin, T. A., et al. (2011). Chronic methamphetamine exposure suppresses the striatal expression of members of multiple families of immediate early genes (IEGs) in the rat: normalization by an acute methamphetamine injection. Psychopharmacology (Berl.) 215, 353–365. doi: 10.1007/s00213-010-2146-7
McMorrow, J. P., and Murphy, E. P. (2011). Inflammation: a role for NR4A orphan nuclear receptors? Biochem. Soc. Trans. 39, 688–693. doi: 10.1042/BST0390688
Milbrandt, J. (1988). Nerve growth factor induces a gene homologous to the glucocorticoid receptor gene. Neuron 1, 183–188. doi: 10.1016/0896-6273(88)90138-9
Moga, M. M., and Saper, C. B. (1994). Neuropeptide-immunoreactive neurons projecting to the paraventricular hypothalamic nucleus in the rat. J. Comp. Neurol. 346, 137–150. doi: 10.1002/cne.903460110
Moga, M. M., Saper, C. B., and Gray, T. S. (1989). Bed nucleus of the stria terminalis: cytoarchitecture, immunohistochemistry, and projection to the parabrachial nucleus in the rat. J. Comp. Neurol. 283, 315–332. doi: 10.1002/cne.902830302
Mohan, H. M., Aherne, C. M., Rogers, A. C., Baird, A. W., Winter, D. C., and Murphy, E. P. (2012). Molecular pathways: the role of NR4A orphan nuclear receptors in cancer. Clin. Cancer Res. 18, 3223–3228. doi: 10.1158/1078-0432.CCR-11-2953
Moore, T. M., Brown, T., Cade, M., and Eells, J. B. (2008). Alterations in amphetamine-stimulated dopamine overflow due to the Nurr1-null heterozygous genotype and postweaning isolation. Synapse 62, 764–774. doi: 10.1002/syn.20550
Mullican, S. E., Zhang, S., Konopleva, M., Ruvolo, V., Andreeff, M., Milbrandt, J., et al. (2007). Abrogation of nuclear receptors Nr4a3 and Nr4a1 leads to development of acute myeloid leukemia. Nat. Med. 13, 730–735. doi: 10.1038/nm1579
Murphy, E. P., and Conneely, O. M. (1997). Neuroendocrine regulation of the hypothalamic pituitary adrenal axis by the nurr1/nur77 subfamily of nuclear receptors. Mol. Endocrinol. 11, 39–47. doi: 10.1210/me.11.1.39
Murphy, E. P., Dobson, A. D., Keller, C., and Conneely, O. M. (1996). Differential regulation of transcription by the NURR1/NUR77 subfamily of nuclear transcription factors. Gene Expr. 5, 169–179.
Nielsen, D. A., Ji, F., Yuferov, V., Ho, A., Chen, A., Levran, O., et al. (2008). Genotype patterns that contribute to increased risk for or protection from developing heroin addiction. Mol. Psychiatry 13, 417–428. doi: 10.1038/sj.mp.4002147
Nordzell, M., Aarnisalo, P., Benoit, G., Castro, D. S., and Perlmann, T. (2004). Defining an N-terminal activation domain of the orphan nuclear receptor Nurr1. Biochem. Biophys. Res. Commun. 313, 205–211. doi: 10.1016/j.bbrc.2003.11.079
Ohkura, N., Ito, M., Tsukada, T., Sasaki, K., Yamaguchi, K., and Miki, K. (1996). Structure, mapping and expression of a human NOR-1 gene, the third member of the Nur77/NGFI-B family. Biochim. Biophys. Acta 1308, 205–214. doi: 10.1016/0167-4781(96)00101-7
Ojeda, V., Fuentealba, J. A., Galleguillos, D., and Andres, M. E. (2003). Rapid increase of Nurr1 expression in the substantia nigra after 6-hydroxydopamine lesion in the striatum of the rat. J. Neurosci. Res. 73, 686–697. doi: 10.1002/jnr.10705
Parkes, D., Rivest, S., Lee, S., Rivier, C., and Vale, W. (1993). Corticotropin-releasing factor activates c-fos, NGFI-B, and corticotropin-releasing factor gene expression within the paraventricular nucleus of the rat hypothalamus. Mol. Endocrinol. 7, 1357–1367. doi: 10.1210/me.7.10.1357
Paulsen, R. F., Granas, K., Johnsen, H., Rolseth, V., and Sterri, S. (1995). Three related brain nuclear receptors, NGFI-B, Nurr1, and NOR-1, as transcriptional activators. J. Mol. Neurosci. 6, 249–255. doi: 10.1007/BF02736784
Pelton, G. H., Lee, Y., and Davis, M. (1997). Repeated stress, like vasopressin, sensitizes the excitatory effects of corticotropin releasing factor on the acoustic startle reflex. Brain Res. 778, 381–387. doi: 10.1016/S0006-8993(97)00669-0
Perlmann, T., and Jansson, L. (1995). A novel pathway for vitamin A signaling mediated by RXR heterodimerization with NGFI-B and NURR1. Genes Dev. 9, 769–782. doi: 10.1101/gad.9.7.769
Perlmann, T., and Wallen-Mackenzie, A. (2004). Nurr1, an orphan nuclear receptor with essential functions in developing dopamine cells. Cell Tissue Res. 318, 45–52. doi: 10.1007/s00441-004-0974-7
Philips, A., Lesage, S., Gingras, R., Maira, M. H., Gauthier, Y., Hugo, P., et al. (1997). Novel dimeric Nur77 signaling mechanism in endocrine and lymphoid cells. Mol. Cell. Biol. 17, 5946–5951.
Ponnio, T., Burton, Q., Pereira, F. A., Wu, D. K., and Conneely, O. M. (2002). The nuclear receptor Nor-1 is essential for proliferation of the semicircular canals of the mouse inner ear. Mol. Cell. Biol. 22, 935–945. doi: 10.1128/MCB.22.3.935-945.2002
Ponnio, T., and Conneely, O. M. (2004). nor-1 regulates hippocampal axon guidance, pyramidal cell survival, and seizure susceptibility. Mol. Cell. Biol. 24, 9070–9078. doi: 10.1128/MCB.24.20.9070-9078.2004
Rogge, G. A., Singh, H., Dang, R., and Wood, M. A. (2013). HDAC3 is a negative regulator of cocaine-context-associated memory formation. J. Neurosci. 33, 6623–6632. doi: 10.1523/JNEUROSCI.4472-12.2013
Rojas, P., Joodmardi, E., Hong, Y., Perlmann, T., and Ogren, S. O. (2007). Adult mice with reduced Nurr1 expression: an animal model for schizophrenia. Mol. Psychiatry 12, 756–766. doi: 10.1038/sj.mp.4001993
Rojas, P., Joodmardi, E., Perlmann, T., and Ogren, S. O. (2010). Rapid increase of Nurr1 mRNA expression in limbic and cortical brain structures related to coping with depression-like behavior in mice. J. Neurosci. Res. 88, 2284–2293. doi: 10.1002/jnr.22377
Sacchetti, P., Carpentier, R., Segard, P., Olive-Cren, C., and Lefebvre, P. (2006). Multiple signaling pathways regulate the transcriptional activity of the orphan nuclear receptor NURR1. Nucleic Acids Res. 34, 5515–5527. doi: 10.1093/nar/gkl712
Sacchetti, P., Mitchell, T. R., Granneman, J. G., and Bannon, M. J. (2001). Nurr1 enhances transcription of the human dopamine transporter gene through a novel mechanism. J. Neurochem. 76, 1565–1572. doi: 10.1046/j.1471-4159.2001.00181.x
Saijo, K., Winner, B., Carson, C. T., Collier, J. G., Boyer, L., Rosenfeld, M. G., et al. (2009). A Nurr1/CoREST pathway in microglia and astrocytes protects dopaminergic neurons from inflammation-induced death. Cell 137, 47–59. doi: 10.1016/j.cell.2009.01.038
Sakanaka, M., Shibasaki, T., and Lederis, K. (1986). Distribution and efferent projections of corticotropin-releasing factor-like immunoreactivity in the rat amygdaloid complex. Brain Res. 382, 213–238. doi: 10.1016/0006-8993(86)91332-6
Sakurada, K., Ohshima-Sakurada, M., Palmer, T. D., and Gage, F. H. (1999). Nurr1, an orphan nuclear receptor, is a transcriptional activator of endogenous tyrosine hydroxylase in neural progenitor cells derived from the adult brain. Development 126, 4017–4026.
Santibanez, M., Gysling, K., and Forray, M. I. (2006). Desipramine prevents the sustained increase in corticotropin-releasing hormone-like immunoreactivity induced by repeated immobilization stress in the rat central extended amygdala. J. Neurosci. Res. 84, 1270–1281. doi: 10.1002/jnr.21023
Satoh, J., and Kuroda, Y. (2002). The constitutive and inducible expression of Nurr1, a key regulator of dopaminergic neuronal differentiation, in human neural and non-neural cell lines. Neuropathology 22, 219–232. doi: 10.1046/j.1440-1789.2002.00460.x
Saucedo-Cardenas, O., and Conneely, O. M. (1996). Comparative distribution of NURR1 and NUR77 nuclear receptors in the mouse central nervous system. J. Mol. Neurosci. 7, 51–63. doi: 10.1007/BF02736848
Saucedo-Cardenas, O., Quintana-Hau, J. D., Le, W. D., Smidt, M. P., Cox, J. J., De Mayo, F., et al. (1998). Nurr1 is essential for the induction of the dopaminergic phenotype and the survival of ventral mesencephalic late dopaminergic precursor neurons. Proc. Natl. Acad. Sci. U.S.A. 95, 4013–4018. doi: 10.1073/pnas.95.7.4013
Sawchenko, P. E., and Swanson, L. W. (1985). Localization, colocalization, and plasticity of corticotropin-releasing factor immunoreactivity in rat brain. Fed. Proc. 44, 221–227.
Schiltz, C. A., Kelley, A. E., and Landry, C. F. (2007). Acute stress and nicotine cues interact to unveil locomotor arousal and activity-dependent gene expression in the prefrontal cortex. Biol. Psychiatry 61, 127–135. doi: 10.1016/j.biopsych.2006.03.002
Silverman, A. J., Hou-Yu, A., and Chen, W. P. (1989). Corticotropin-releasing factor synapses within the paraventricular nucleus of the hypothalamus. Neuroendocrinology 49, 291–299. doi: 10.1159/000125131
Sinha, R. (2008). Chronic stress, drug use, and vulnerability to addiction. Ann. N.Y. Acad. Sci. 1141, 105–130. doi: 10.1196/annals.1441.030
Slagsvold, H. H., Ostvold, A. C., Fallgren, A. B., and Paulsen, R. E. (2002). Nuclear receptor and apoptosis initiator NGFI-B is a substrate for kinase ERK2. Biochem. Biophys. Res. Commun. 291, 1146–1150. doi: 10.1006/bbrc.2002.6579
Smidt, M. P., Smits, S. M., and Burbach, J. P. (2003). Molecular mechanisms underlying midbrain dopamine neuron development and function. Eur. J. Pharmacol. 480, 75–88. doi: 10.1016/j.ejphar.2003.08.094
Smits, S. M., Ponnio, T., Conneely, O. M., Burbach, J. P., and Smidt, M. P. (2003). Involvement of Nurr1 in specifying the neurotransmitter identity of ventral midbrain dopaminergic neurons. Eur. J. Neurosci. 18, 1731–1738. doi: 10.1046/j.1460-9568.2003.02885.x
Sohn, S. J., Thompson, J., and Winoto, A. (2007). Apoptosis during negative selection of autoreactive thymocytes. Curr. Opin. Immunol. 19, 510–515. doi: 10.1016/j.coi.2007.06.001
Sohn, Y. C., Kwak, E., Na, Y., Lee, J. W., and Lee, S. K. (2001). Silencing mediator of retinoid and thyroid hormone receptors and activating signal cointegrator-2 as transcriptional coregulators of the orphan nuclear receptor Nur77. J. Biol. Chem. 276, 43734–43739. doi: 10.1074/jbc.M107208200
Stenzel-Poore, M. P., Heinrichs, S. C., Rivest, S., Koob, G. F., and Vale, W. W. (1994). Overproduction of corticotropin-releasing factor in transgenic mice: a genetic model of anxiogenic behavior. J. Neurosci. 14, 2579–2584.
St-Hilaire, M., Bourhis, E., Levesque, D., and Rouillard, C. (2006). Impaired behavioural and molecular adaptations to dopamine denervation and repeated L-DOPA treatment in Nur77-knockout mice. Eur. J. Neurosci. 24, 795–805. doi: 10.1111/j.1460-9568.2006.04954.x
St-Hilaire, M., Landry, E., Levesque, D., and Rouillard, C. (2003a). Denervation and repeated L-DOPA induce a coordinate expression of the transcription factor NGFI-B in striatal projection pathways in hemi-parkinsonian rats. Neurobiol. Dis. 14, 98–109. doi: 10.1016/S0969-9961(03)00081-0
St-Hilaire, M., Tremblay, P. O., Levesque, D., Barden, N., and Rouillard, C. (2003b). Effects of cocaine on c-fos and NGFI-B mRNA expression in transgenic mice underexpressing glucocorticoid receptors. Neuropsychopharmacology 28, 478–489. doi: 10.1038/sj.npp.1300067
St-Hilaire, M., Landry, E., Levesque, D., and Rouillard, C. (2005). Denervation and repeated L-DOPA induce complex regulatory changes in neurochemical phenotypes of striatal neurons: implication of a dopamine D1-dependent mechanism. Neurobiol. Dis. 20, 450–460. doi: 10.1016/j.nbd.2005.04.001
Stout, S. C., Mortas, P., Owens, M. J., Nemeroff, C. B., and Moreau, J. (2000). Increased corticotropin-releasing factor concentrations in the bed nucleus of the stria terminalis of anhedonic rats. Eur. J. Pharmacol. 401, 39–46. doi: 10.1016/S0014-2999(00)00412-X
Stroth, N., Liu, Y., Aguilera, G., and Eiden, L. E. (2011). Pituitary adenylate cyclase-activating polypeptide controls stimulus-transcription coupling in the hypothalamic-pituitary-adrenal axis to mediate sustained hormone secretion during stress. J. Neuroendocrinol. 23, 944–955. doi: 10.1111/j.1365-2826.2011.02202.x
Sun, W., Choi, S. H., Park, S. K., Kim, S. J., Noh, M. R., Kim, E. H., et al. (2007). Identification and characterization of novel activity-dependent transcription factors in rat cortical neurons. J. Neurochem. 100, 269–278. doi: 10.1111/j.1471-4159.2006.04214.x
Svenningsson, P., and Fredholm, B. B. (1997). Caffeine mimics the effect of a dopamine D2/3 receptor agonist on the expression of immediate early genes in globus pallidus. Neuropharmacology 36, 1309–1317. doi: 10.1016/S0028-3908(97)00091-9
Svenningsson, P., Nomikos, G. G., and Fredholm, B. B. (1995). Biphasic changes in locomotor behavior and in expression of mRNA for NGFI-A and NGFI-B in rat striatum following acute caffeine administration. J. Neurosci. 15, 7612–7624.
Swanson, L. W., and Simmons, D. M. (1989). Differential steroid hormone and neural influences on peptide mRNA levels in CRH cells of the paraventricular nucleus: a hybridization histochemical study in the rat. J. Comp. Neurol. 285, 413–435. doi: 10.1002/cne.902850402
Tang, Y. P., Murata, Y., Nagaya, T., Noda, Y., Seo, H., and Nabeshima, T. (1997). NGFI-B, c-fos, and c-jun mRNA expression in mouse brain after acute carbon monoxide intoxication. J. Cereb. Blood Flow Metab. 17, 771–780. doi: 10.1097/00004647-199707000-00007
Tseng, K. Y., Roubert, C., Do, L., Rubinstein, M., Kelly, M. A., Grandy, D. K., et al. (2000). Selective increase of Nurr1 mRNA expression in mesencephalic dopaminergic neurons of D2 dopamine receptor-deficient mice. Brain Res. Mol. Brain Res. 80, 1–6. doi: 10.1016/S0169-328X(00)00107-8
Umemoto, S., Kawai, Y., and Senba, E. (1994). Differential regulation of IEGs in the rat PVH in single and repeated stress models. Neuroreport 6, 201–204. doi: 10.1097/00001756-199412300-00051
Umemoto, S., Kawai, Y., Ueyama, T., and Senba, E. (1997). Chronic glucocorticoid administration as well as repeated stress affects the subsequent acute immobilization stress-induced expression of immediate early genes but not that of NGFI-A. Neuroscience 80, 763–773. doi: 10.1016/S0306-4522(97)00050-X
Valjent, E., Bertran-Gonzalez, J., Herve, D., Fisone, G., and Girault, J. A. (2009). Looking BAC at striatal signaling: cell-specific analysis in new transgenic mice. Trends Neurosci. 32, 538–547. doi: 10.1016/j.tins.2009.06.005
van Tiel, C. M., and de Vries, C. J. (2012). NR4All in the vessel wall. J. Steroid Biochem. Mol. Biol. 130, 186–193. doi: 10.1016/j.jsbmb.2011.01.010
van Tiel, C. M., Kurakula, K., Koenis, D. S., van der Wal, E., and de Vries, C. J. (2012). Dual function of Pin1 in NR4A nuclear receptor activation: enhanced activity of NR4As and increased Nur77 protein stability. Biochim. Biophys. Acta 1823, 1894–1904. doi: 10.1016/j.bbamcr.2012.06.030
Veinante, P., Stoeckel, M. E., and Freund-Mercier, M. J. (1997). GABA- and peptide-immunoreactivities co-localize in the rat central extended amygdala. Neuroreport 8, 2985–2989. doi: 10.1097/00001756-199709080-00035
Vuillermot, S., Joodmardi, E., Perlmann, T., Ove Ogren, S., Feldon, J., and Meyer, U. (2011). Schizophrenia-relevant behaviors in a genetic mouse model of constitutive Nurr1 deficiency. Genes Brain Behav. 10, 589–603. doi: 10.1111/j.1601-183X.2011.00698.x
Walker, D. L., Miles, L. A., and Davis, M. (2009). Selective participation of the bed nucleus of the stria terminalis and CRF in sustained anxiety-like versus phasic fear-like responses. Prog. Neuropsychopharmacol. Biol. Psychiatry 33, 1291–1308. doi: 10.1016/j.pnpbp.2009.06.022
Walker, D. L., Toufexis, D. J., and Davis, M. (2003). Role of the bed nucleus of the stria terminalis versus the amygdala in fear, stress, and anxiety. Eur. J. Pharmacol. 463, 199–216. doi: 10.1016/S0014-2999(03)01282-2
Wallen, A. A., Castro, D. S., Zetterstrom, R. H., Karlen, M., Olson, L., Ericson, J., et al. (2001). Orphan nuclear receptor Nurr1 is essential for Ret expression in midbrain dopamine neurons and in the brain stem. Mol. Cell. Neurosci. 18, 649–663. doi: 10.1006/mcne.2001.1057
Wang, Z., Benoit, G., Liu, J., Prasad, S., Aarnisalo, P., Liu, X., et al. (2003). Structure and function of Nurr1 identifies a class of ligand-independent nuclear receptors. Nature 423, 555–560. doi: 10.1038/nature01645
Werme, M., Hermanson, E., Carmine, A., Buervenich, S., Zetterstrom, R. H., Thoren, P., et al. (2003). Decreased ethanol preference and wheel running in Nurr1-deficient mice. Eur. J. Neurosci. 17, 2418–2424. doi: 10.1046/j.1460-9568.2003.02666.x
Werme, M., Olson, L., and Brene, S. (2000a). NGFI-B and nor1 mRNAs are upregulated in brain reward pathways by drugs of abuse: different effects in Fischer and Lewis rats. Brain Res. Mol. Brain Res. 76, 18–24. doi: 10.1016/S0169-328X(99)00327-7
Werme, M., Ringholm, A., Olson, L., and Brene, S. (2000b). Differential patterns of induction of NGFI-B, Nor1 and c-fos mRNAs in striatal subregions by haloperidol and clozapine. Brain Res. 863, 112–119. doi: 10.1016/S0006-8993(00)02109-0
Werme, M., Thoren, P., Olson, L., and Brene, S. (1999). Addiction-prone Lewis but not Fischer rats develop compulsive running that coincides with downregulation of nerve growth factor inducible-B and neuron-derived orphan receptor 1. J. Neurosci. 19, 6169–6174.
Williams, G. T., and Lau, L. F. (1993). Activation of the inducible orphan receptor gene nur77 by serum growth factors: dissociation of immediate-early and delayed-early responses. Mol. Cell. Biol. 13, 6124–6136. doi: 10.1128/MCB.13.10.6124
Wilson, T. E., Fahrner, T. J., Johnston, M., and Milbrandt, J. (1991). Identification of the DNA binding site for NGFI-B by genetic selection in yeast. Science 252, 1296–1300. doi: 10.1126/science.1925541
Wilson, T. E., Fahrner, T. J., and Milbrandt, J. (1993a). The orphan receptors NGFI-B and steroidogenic factor 1 establish monomer binding as a third paradigm of nuclear receptor-DNA interaction. Mol. Cell. Biol. 13, 5794–5804. doi: 10.1128/MCB.13.9.5794
Wilson, T. E., Mouw, A. R., Weaver, C. A., Milbrandt, J., and Parker, K. L. (1993b). The orphan nuclear receptor NGFI-B regulates expression of the gene encoding steroid 21-hydroxylase. Mol. Cell. Biol. 13, 861–868. doi: 10.1128/MCB.13.2.861
Wingate, A. D., Campbell, D. G., Peggie, M., and Arthur, J. S. (2006). Nur77 is phosphorylated in cells by RSK in response to mitogenic stimulation. Biochem. J. 393, 715–724. doi: 10.1042/BJ20050967
Xiao, Q., Castillo, S. O., and Nikodem, V. M. (1996). Distribution of messenger RNAs for the orphan nuclear receptors Nurr1 and Nur77 (NGFI-B) in adult rat brain using in situ hybridization. Neuroscience 75, 221–230. doi: 10.1016/0306-4522(96)00159-5
Xing, G., Zhang, L., Zhang, L., Heynen, T., Li, X. L., Smith, M. A., et al. (1997). Rat nurr1 is prominently expressed in perirhinal cortex, and differentially induced in the hippocampal dentate gyrus by electroconvulsive vs. kindled seizures. Brain Res. Mol. Brain Res. 47, 251–261. doi: 10.1016/S0169-328X(97)00056-9
Yao, M., and Denver, R. J. (2007). Regulation of vertebrate corticotropin-releasing factor genes. Gen. Comp. Endocrinol. 153, 200–216. doi: 10.1016/j.ygcen.2007.01.046
Yao, M., Stenzel-Poore, M., and Denver, R. J. (2007). Structural and functional conservation of vertebrate corticotropin-releasing factor genes: evidence for a critical role for a conserved cyclic AMP response element. Endocrinology 148, 2518–2531. doi: 10.1210/en.2006-1413
Zetterstrom, R. H., Solomin, L., Jansson, L., Hoffer, B. J., Olson, L., and Perlmann, T. (1997). Dopamine neuron agenesis in Nurr1-deficient mice. Science 276, 248–250. doi: 10.1126/science.276.5310.248
Zetterstrom, R. H., Solomin, L., Mitsiadis, T., Olson, L., and Perlmann, T. (1996a). Retinoid X receptor heterodimerization and developmental expression distinguish the orphan nuclear receptors NGFI-B, Nurr1, and Nor1. Mol. Endocrinol. 10, 1656–1666. doi: 10.1210/me.10.12.1656
Zetterstrom, R. H., Williams, R., Perlmann, T., and Olson, L. (1996b). Cellular expression of the immediate early transcription factors Nurr1 and NGFI-B suggests a gene regulatory role in several brain regions including the nigrostriatal dopamine system. Brain Res. Mol. Brain Res. 41, 111–120. doi: 10.1016/0169-328X(96)00074-5
Zhang, L., Le, W., Xie, W., and Dani, J. A. (2012). Age-related changes in dopamine signaling in Nurr1 deficient mice as a model of Parkinson's disease. Neurobiol. Aging 33, 1001.e1007–1001.e1016. doi: 10.1016/j.neurobiolaging.2011.03.022
Zhang, T., Jia, N., Fei, E., Wang, P., Liao, Z., Ding, L., et al. (2007). Nurr1 is phosphorylated by ERK2 in vitro and its phosphorylation upregulates tyrosine hydroxylase expression in SH-SY5Y cells. Neurosci. Lett. 423, 118–122. doi: 10.1016/j.neulet.2007.06.041
Keywords: Nurr1, Nur77, Nor1, corticotropin releasing factor, addiction, stress, nuclear receptors, gene expression regulation
Citation: Campos-Melo D, Galleguillos D, Sánchez N, Gysling K and Andrés ME (2013) Nur transcription factors in stress and addiction. Front. Mol. Neurosci. 6:44. doi: 10.3389/fnmol.2013.00044
Received: 24 September 2013; Accepted: 09 November 2013;
Published online: 02 December 2013.
Edited by:
Nicola Maggio, The Chaim Sheba Medical Center, IsraelReviewed by:
Ted Abel, University of Pennsylvania, USAIzhak Michaelevski, Tel Aviv University, Israel
Copyright © 2013 Campos-Melo, Galleguillos, Sánchez, Gysling and Andrés. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: María E. Andrés, Nucleus Millennium in Stress and Addiction, Department of Cellular and Molecular Biology, Faculty of Biological Sciences, Pontificia Universidad Católica de Chile, PO Box 114D, Santiago 8331150, Chile e-mail: mandres@bio.puc.cl
†Present address: Danae Campos-Melo, Molecular Medicine Group, Robarts Research Institute, Western University, London, Canada;
Danny Galleguillos, Department of Pharmacology, University of Alberta, Edmonton, Canada