Neurological manifestations of encephalitic alphaviruses, traumatic brain injuries, and organophosphorus nerve agent exposure

Encephalitic alphaviruses (EEVs), Traumatic Brain Injuries (TBI), and organophosphorus nerve agents (NAs) are three diverse biological, physical, and chemical injuries that can lead to long-term neurological deficits in humans. EEVs include Venezuelan, eastern, and western equine encephalitis viruses. This review describes the current understanding of neurological pathology during these three conditions, provides a comparative review of case studies vs. animal models, and summarizes current therapeutics. While epidemiological data on clinical and pathological manifestations of these conditions are known in humans, much of our current mechanistic understanding relies upon animal models. Here we review the animal models findings for EEVs, TBIs, and NAs and compare these with what is known from human case studies. Additionally, research on NAs and EEVs is limited due to their classification as high-risk pathogens (BSL-3) and/or select agents; therefore, we leverage commonalities with TBI to develop a further understanding of the mechanisms of neurological damage. Furthermore, we discuss overlapping neurological damage mechanisms between TBI, NAs, and EEVs that highlight novel medical countermeasure opportunities. We describe current treatment methods for reducing neurological damage induced by individual conditions and general neuroprotective treatment options. Finally, we discuss perspectives on the future of neuroprotective drug development against long-term neurological sequelae of EEVs, TBIs, and NAs.

1 Introduction and global impact

1.1 EEVs

The equine encephalitis complex, including western, eastern, and Venezuelan equine encephalitis viruses (WEEV, EEEV, and VEEV), are New World alphaviruses in the family Togaviridae (Aguilar et al., 2011; Calisher, 1994). These viruses are collectively referred to as equine encephalitic viruses (EEVs) throughout this manuscript. These viruses are maintained in an enzootic cycle between mosquitoes and an animal host (e.g., rodents and/or birds) but can spill over into both humans and horses, causing illness (Ronca et al., 2016). In horses, mules, and donkey's, equine encephalitis can cause appetite loss, flu-like symptoms, and progress to muscle and nervous system degeneration (Guzmán-Terán et al., 2020). In humans, illness can include flu-like symptoms and can also progress to neurological deficits and seizures. These effects occur most commonly in children for whom EEVs are more likely to cause life-long behavioral deficits, seizures, and delirium (Falchek, 2012). Cases that progress to neurological deficits, including encephalitis require significant long-term care, which was estimated to cost $320,000 per individual in 1971, and $400,000 per year per individual in 1995 (Villari et al., 1995; Earnest et al., 1971). Based on the 2.54% inflation of the economy between 1995 and 2023 (Webster, 2024), this equates to ~$790,000 per year in 2023. Despite over 100 years of research on these viruses, there are no FDA-approved antiviral treatments or vaccines against EEV infection in humans. Ultimately, current treatment options are limited to supportive care. The lack of FDA approved therapeutics for EEVs is due to a variety of challenges, including the limited number of laboratories researching these viruses because of biosafety level limitations and the lack of adequate and standardized animal models.

1.2 TBI

Traumatic brain injury (TBI) is a leading cause of death and disability across the world, with an estimated 69 million people sustaining an injury each year (Dewan et al., 2018; Peterson et al., 2022). The CDC defines a TBI as “an injury that affects how the brain works” and may be caused by a “bump, blow, or jolt to the head, or a penetrating injury” (CDC, 2023a). Following the primary insult, “secondary injury” will occur in the minutes, hours, days, weeks, and years' post-injury. Secondary injury leads to chronic neurological deficits due to cell death, inflammation, and other consequences of primary injury. Additionally, TBI is a risk factor for several neurodegenerative disorders and neurological disorders and impairments (Dams-O'Connor et al., 2016; Delic et al., 2020; Rapoport, 2012; Howlett et al., 2022). Both primary and secondary injury lead to acute seizures in 1/5 individuals who receive a TBI and chronic post-traumatic epilepsy (PTE) occurs in ~1/50 cases (Fordington and Manford, 2020). Due to the heterogeneous nature of the TBI mechanism, pathophysiology, severity, and outcome, developing effective therapeutic strategies and treatments has yielded limited success, with no correct or universal FDA-approved treatments available (Kochanek et al., 2020; Nishimura et al., 2022). Several recent review articles and reports cover new technological advances in TBI research (Bowman et al., 2022; Bonanno et al., 2022; Lu et al., 2015; Ahmed, 2022). These focus on developments in imaging, biomarkers, and therapeutic approaches that offer insights into the pathophysiology and treatment of TBI. For instance, innovations in high-density neurophysiology monitoring systems, alongside advanced neuroimaging and bioinformatics, have enabled more precise tracking of brain injury progression and recovery. Technologies like high-resolution MRI, PET imaging, and blood-based biomarkers are helping researchers characterize injury severity and predict outcomes more accurately. Therefore, novel approaches to studying TBI and its related pathophysiology may enable therapeutic development.

1.3 NAs

Organophosphate compounds (OPs) are primarily divided into nerve agent OPs and pesticide OPs. Exposure to OPs causes the death of 300,000 people per year worldwide (Ahmad et al., 2024; Adeyinka et al., 2024), primarily from occupational exposure to pesticide OPs and exposures in underdeveloped countries. Challenges in identification of agent in a warfare setting make estimation of deadly nerve agent OP exposures difficult and likely underestimated (Costanzi et al., 2018; Gunnell et al., 2007) though there are recent high-profile examples of nerve agent OP use on civilians (Chai et al., 2017; Haslam et al., 2022; John et al., 2018; Morita et al., 1995). For this review we will focus on nerve agents (NAs) as these have significant implications as warfare agents (Mukherjee and Gupta, 2020). NAs irreversibly inhibit acetylcholinesterase activity, causing a buildup of acetylcholine in both the central and peripheral nervous systems that produces significant neurological changes (Costanzi et al., 2018). NAs are typically broken into classes, including the “German” G-series agents sarin (GB), soman (GD), tabun (GA), and the Russian “Venomous, Victory, or Viscous” V-series agents, which consists of VE, VG, VM, VR, and VX. Nerve agent mechanisms of action are consistent across the series, but the volatility and toxicity vary. V-series nerve agents, most notably VX, are less volatile at ambient temperature and generally regarded as more toxic than G-series agents, but this toxicity is typically associated with skin contact and persistent environmental hazard (Jang et al., 2015; Wiener and Hoffman, 2004; Rosenblatt et al., 1996). G-series agents are volatile at room temperature leading to more significant toxicity through inhalation or skin contact. An additional class, the Novichoks and A-series agents, will not be discussed in this review as their classification, chemical structures, and mechanisms are not well characterized by the literature. The little information that is present concerning this series is incomplete and from scrutinized sources, whereas G-series and V-series agents are relatively well characterized in literature. A-series and Novichok agents in particular display an extreme level of toxicity not observed in other nerve agents (Opravil et al., 2023; Noga and Jurowski, 2023).

Exposure to NAs can occur through inhalation, ingestion, or skin absorption (Wiercinski and Jackson, 2024). Small amounts of these chemicals can cause a variety of mild to moderate flu-like symptoms such as nausea, vomiting, confusion, headache, and weakness or other cholinergic symptoms such as watering eyes, drooling, blurred vision, increased heart rate, muscle spasms, and sweating (CDC, 2023b). The onset of symptoms is typically rapid at high doses, but long-term low dosage exposure can induce memory impairment, motor dysfunction, depression, and anxiety, with multiple lines of evidence from Tokyo Subway Attack victims, veterans, and exposed Iraqi civilians (Figueiredo et al., 2018). The mechanism by which long-term low-dose exposure of NA induces depression and behavioral changes is not well understood: however, neuronal damage and degeneration is commonly correlated with cognitive and motor dysfunction observed post-mortem (Figueiredo et al., 2018). Lower doses of NAs also can cause convulsion mediated at peripheral neuromuscular junctions, high levels of NA exposure almost invariably result in prolonged or repetitive central seizures (status epilepticus, SE) or death without immediate medical intervention (Hrvat and Kovarik, 2020). Unique to NAs, most long-term neurological changes are due to SE-induced neuronal damage, inflammation, and changes to neuronal signaling (Figueiredo et al., 2018) as NAs do not have directly toxic effects on neural cells (Aroniadou-Anderjaska et al., 2023).

2 Neuropathology in humans and laboratory animal models

2.1 EEVs

Human case studies of VEEV, EEEV, and WEEV are extensively under-documented due to the initial symptoms often presenting as febrile illness as well as non-specific documentation due to these viral illnesses being classified under a larger umbrella of neuroinvasive viruses, including flaviviruses, West Nile virus (WNV), and Japanese encephalitis virus (JEV) (Calisher, 1994; Ronca et al., 2016). The number of reported VEEV, WEEV, and EEEV cases trace back to the 1930s, the same time frame in which these viruses were isolated and mosquito vectors were determined to be the route of transmission (Weaver et al., 2004a; Zacks and Paessler, 2010). The combined impact of WEEV, VEEV, and EEEV includes >300,000 cases, >300 recorded deaths, and 3,000 survivors with long-lasting neurological disorders, including paralysis, seizures, migraines, and depression (Crosby and Crespo, 2024) (Table 1). As previously mentioned, this is likely an underestimate due to many viral infections causing vague symptoms in humans, and therefore these infections may go undiagnosed and untested. A recent study identified elevated glial fibrillary acidic protein (GFAP) in patient serum from acute VEEV and Madariaga virus (MADV) infections; however this biomarker was not specific to alphavirus infection as bacterial cases with encephalitis also displayed elevated GFAP (Bartlett et al., 2024). EEV pathology in humans has been divided into three distinct phases consisting of a lymphotropic phase (early/short term), a neuroinvasive phase, and a neurodegenerative phase (late/long-term) [reviewed in Steele and Twenhafel (2010); Kehn-Hall and Bradfute (2022)]. Neurological complications can be attributed to both the neuroinvasion and replication of EEVs in the brain, as well as from the inflammatory response. Despite the large range of neurological deficits induced by these viruses, there is little known about the mechanism of neuroinvasion in humans. Similarly, it is debated whether the inflammatory process hallmarked by the increase of neutrophils in the central nervous system (CNS) is harmful or helpful (Peiseler and Kubes, 2019; Drescher and Bai, 2013). Therefore, we rely upon animal models of infection to study pathologic, behavioral, and molecular processes altered by infection and ultimately identifying therapeutics. Animal models of EEVs have previously been reviewed (Ronca et al., 2016; Steele and Twenhafel, 2010; Kehn-Hall and Bradfute, 2022); however, here, we seek to highlight the consequences of neuroinvasion and long-term neurological symptoms of EEVs in order to correlate them across different neurological diseases.

Table 1

Table 1. Impacts of EEVs, TBI, and NA on humans.

Animal models used for EEV research include non-human primates, rats, gerbils, mice, guinea pigs, rabbits, and hamsters, which have been extensively reviewed by others (Steele and Twenhafel, 2010; Kehn-Hall and Bradfute, 2022; Dremov and Solianik, 1977). Of these models, Guinea pigs, hamsters, and rabbits have high fatality rates, including severe lymphoid necrosis prior to invasion into the CNS; therefore, they are less ideal models for studying neurological sequelae. In this review, we focus on rodents (i.e. mice, rats, and some hamster models) and non-human primates (NHPs) as animal models of EEV infection, where neurological infiltration has been well established. It's also important to note that studies in animal models have primarily been reliant upon the utilization of a variety of different strains of VEEV, EEEV, and WEEV, including naturally evolving strains (VEEV Subtypes I-VI, VEEV Trinidad Donkey (TrD), EEEV Georgia Fatal, EEEV FL93-939, EEEV North American, WEEV MacMillan (MCM), WEEV IMP 181) as well as some attenuated viral isolates previously reviewed (e.g. VEEV TC-83) (Steele and Twenhafel, 2010; Sharma and Knollmann-Ritschel, 2019). Route of infection is also important to consider as there are multiple infection methods used including intranasal, aerosolization, and subcutaneous infection; however, there are different degrees of morbidity and mortality across the different infection routes. While EEVs are naturally transmitted via mosquito bite, there is a large interest in understanding pathogenesis that results from inhalational exposure. EEVs are readily aerosolized, VEEV was developed as a biological weapon, and a large number of laboratory acquired infections have occurred via VEEV aerosolization (Rusnak et al., 2018; Weaver et al., 2004b).

2.1.1 VEEV

VEEV infections in humans result in a relatively low mortality rate (<1%); however, the progression to neurological deficits is significant with 4%−14% of cases progressing to neurological signs with a higher incidence in children, elderly, and immune compromised people (Lundberg et al., 2017). The two largest outbreaks of VEEV occurred through mosquito transmission in Texas in 1971 and Columbia in 1995 (Aguilar et al., 2011). Collectively, there were >300 deaths reported and 3,000 cases with long-lasting neurological disorders including paralysis, seizures, migraines, and depression (Crosby and Crespo, 2024). Additional symptoms included reduced sensory perception of taste, hearing, and smell and changes in emotional stability and mental fitness (Carrera J. P. et al., 2013; Bowen et al., 1976). These studies highlight significant infection rates and neurological sequelae resulting from VEEV outbreaks.

VEEV infection in NHPs results in clinical symptoms such as weight loss, lethargy, hunching, hyperactivity aggression, photophobia, and full body and partial tremors, which closely resembles infection in humans (Table 2) (Burke et al., 2019). Aerosolized VEEV exposure typically results in prolonged febrile state for 1–8 days post-infection (DPI) with immediate invasion into the brain, whereas subcutaneous administration typically presents as febrile illness for 1–6 DPI with signs of virus in the brain and neurological symptoms such as depression 2–3 DPI (Reed et al., 2004; Weaver et al., 2012; Gleiser et al., 1961; Ludwig et al., 2001). The intranasal route of exposure led to the most severe illness in NHPs with invasion, replication, and lesions due to viral infection as early as 48 h post infection (hpi). Further investigation of infected NHPs identified neuronal necrosis, lesions in the thalamus and olfactory cortex, and perivascular cuffing and gliosis with severe inflammation in the hippocampus and cortex (Reed et al., 2004; Gleiser et al., 1961; Danes et al., 1973; Victor et al., 1956; Smith et al., 2020). In Rhesus Monkeys, vertical transmission via fetal contraction of VEEV was confirmed via intranasal infection of the mother. The fetuses showed viral replication in both the brain and peripheral organs, which led to vision impairment and abnormal brain growth, including microcephaly, hydrocephalus, and porencephaly in 67% of cases (London et al., 1977). Evaluation of VEEV in NHP has several advantages as they display highly similar patterns of febrile illness, lethargy, and depression to human case studies; however, murine models of infection include several cost-effective and higher throughputs benefits not represented in this model.

Table 2

Table 2. Comparison of human EEV cases and laboratory animal models of EEV infection.

Infection in mice via footpad or subcutaneous injection leads to viral replication in the lymphatic system as early as 4 hpi, viremia is detected at 12 hpi, and virus in the brain between 24–72 hpi whereas aerosolization or intranasal infection leads to infection in the brain 16–48 h post-infection (Davis et al., 1994; Rusnak et al., 2019). Multiple strains of mouse models (C57Bl/6, CD-1, BALB/c, C3H/HeN) have been well established with intranasally VEEV-infected mice showing signs of weight loss, tremors, paralysis, and dehydration (Table 2) (Gardner et al., 2008; Berge et al., 1961; Hart et al., 1997, 2000; Vogel et al., 2005; Honnold et al., 2015a,b). In most models of VEEV infection, there is direct entrance into the brain via the olfactory bulb via aerosolization and intranasal routes, which has aided in further understanding the neurological features of infection (Cain et al., 2023; Phillips et al., 2016; Salimi et al., 2020). VEEV can also enter the brain via transcytosis through brain epithelium, independent of blood-brain barrier (BBB) breakdown, which appears later in the course of infection (Salimi et al., 2020). Caveolin-1 was shown to be important for viral neuroinvasion (Salimi et al., 2020). VEEV infection typically results in 100% mortality in mice when they are infected with fully virulent BSL-3 strains of VEEV. However, VEEV TC83 is a live attenuated vaccine strain that is used to study VEEV pathogenesis at BSL-2. VEEV TC83 infection results in different levels of mortality dependent on the strain of mouse utilized. C3H/HeN mice are highly susceptible to VEEV TC83, resulting in 100% mortality when intranasally infected with 10^9.1 and 10^7.1 cell culture infection dose 50 (CCID50) (Julander et al., 2008). It's been proposed that higher mortality rates in VEEV-infected C3H/HeN mice compared to C57BL/6 and BALB/c mice is due to a reduced immune response partially dependent on reduced IgA in C3H/HeN mucosa (Hart et al., 1997; Steele et al., 1998; Charles et al., 1997). The majority of C57BL/6 mice infected intranasally with VEEV TC-83 survive infection and serve as an important model to study neurological sequelae following VEEV infection (Ronca et al., 2017; Cain et al., 2017). These mice display biphasic disease initially causing symptoms such as ruffled fur, weight loss, lethargy, and shivering (Figure 1). Following neuroinvasion, circling, hind limb weakness or paralysis, convulsions, and head-tilt are observed. Post-mortem analysis of VEEV V3000 (molecular clone of fully virulent VEEV TrD) infected mice indicated perivascular cuffing, inclusion bodies, neuronal necrosis, and overt encephalitis widespread across the brain (Sharma and Knollmann-Ritschel, 2019; Cain et al., 2023). It has been confirmed that in mice, VEEV primarily infects neurons, but also infects microglia, macrophages, and oligodendrocytes as well as inducing significant inflammation around the BBB (Sharma and Knollmann-Ritschel, 2019).

Figure 1

Figure 1. Acute and chronic clinical comparisons between EEVs, TBI, and NA. Summary of characterized acute (<30 days post-injury or exposure) and chronic manifestations (>30 days post-exposure) of disease in humans. Overlapping clinical symptoms and sequelae are color-coded. EEV, TBI, and NA (red), TBI and NA shared (magenta), VEEV and NA shared (green), VEEV only (teal), TBI only (blue), and NA (gold). DPI, days post injury/infection. Created in BioRender. Kehn-hall (2024) BioRender.com/r18b546.

There has been a significant effort to determine transcriptomic changes associated with VEEV infection, although much has been done in cell culture with increasing evaluation in murine models. In VEEV-infected mice, neuronal damage has been correlated with upregulation of apoptotic, antiviral, T-cell response, and pro-inflammatory genes including CXCL10, IL1-beta, Interferon gamma (IFN-y), CCL5 (RANTES), CCL3 (MIP-1a) and TNF-alpha (Weaver et al., 2004a; Sharma and Knollmann-Ritschel, 2019; Sharma et al., 2008; Taylor and Paessler, 2013). Symptomatic mice appear to have more significant upregulation of inflammatory genes, as well as signs of increased astrocyte activation within specific areas of the brain, such as the thalamus and hippocampus, even after 6 months post-infection (Ronca et al., 2016). A more expansive study investigated individual areas of the brain on days 1–7 post-infection and identified upregulation of numerous cell death pathways (phagocytic pathways, natural killer, pyroptosis, necroptosis) and pro-inflammatory response (Williams et al., 2023). Collectively, these studies have established that there is much similarity between humans, NHPs, and mice with CNS invasion leading to markers of encephalitis such as perivascular cuffing, neuronal necrosis, as well as other markers of damage including infiltration of leukocytes, brain lesions, and gliosis. Evaluation of proteomic and metabolomic changes is currently limited to cell culture-based studies of VEEV and is thus not included in this review.

2.1.2 EEEV

EEEV infections result in 30%−75% mortality and 50%−90% of surviving individuals progress to long-term neurological symptoms (Lindsey et al., 2020; Ciota, 2022; Langsjoen et al., 2023). Collectively, chronic symptoms are poorly characterized in EEV infected animals due to the high mortality rate. Small periodic cases of EEEV and the emergence of four diverse EEEV lineages from 1960–2010 have yielded inconsistent reporting of morbidity, mortality, and neurological sequelae (Arrigo et al., 2010). There were ~178 documented cases of EEEV infection from 2009–2020 with the majority of these cases in Panama and the eastern United States, with the highest number of cases in Georgia, New Jersey, New York, North Carolina, and Michigan (Carrera J. P. et al., 2013; Virmani et al., 2010; Hill et al., 2023; Vilcarromero et al., 2010). Surveillance efforts identified 100 potential positive cases and seven confirmed cases of EEEV in Panama in 2010, where many survivors experienced chronic seizures which were traced to virally induced abnormalities within the temporal lobe of the brain, responsible for memory storage, formation, sensory processing, and emotions (Carrera J. P. et al., 2013). Sequelae observed in human cases include behavioral, memory, and emotional changes which are potentially attributed to changes such as neuronal loss, vasculitis, thrombosis, gliosis in motor neurons, and abnormalities in the temporal lobe (Table 2) (Ronca et al., 2016; Reddy et al., 2008).

In NHP models of EEEV infection, high fatality rates have been recorded in correlation with the high fatality rates seen in humans (Williams et al., 2022). Studies in NHPs have identified EEEV replication in the CNS and severe neurological disease (Albe et al., 2021; Ma et al., 2022). NHPs infected with EEEV display neuronal dysfunction in the olfactory bulb, olfactory tract, and spinal cord which is associated with EEEV pathology rather than neuronal death, which is also typically seen in VEEV. An overall review of NHP EEEV infections has identified pathological evidence of vasculitis, perivascular cuffing, edema, hemorrhage, and widespread necrosis widespread across the brain (Steele and Twenhafel, 2010; Reed et al., 2004; Arrigo et al., 2010). Another unique characteristic of EEEV in Cynomolgus macaques is the neuroinvasion and pathology in the brain is equally as severe between both intranasal and subcutaneous injection, potentially due to EEEV entering the brain via the bloodstream rather than olfactory nerve routes (Smith et al., 2020).

Mouse models of EEEV are also relatively understudied. EEEV is highly fatal in mice, typically 100% in all routes of infection with accompanying invasion into the brain even with subcutaneous methods as early as 24 h post-infection (Vogel et al., 2005). EEEV infected mice show preferential infection of osteoblasts, skeletal muscle, and fibroblasts, with less efficient replication in macrophages and dendritic cells (Steele and Twenhafel, 2010; Gardner et al., 2008; Vogel et al., 2005). Cytokine analysis indicated CCL5 (RANTES), CXCL9 (MIG), CCL4 (MIP-1B), and IFN-y peak between day 1 and 2 post infection dependent on the route of infection (Honnold et al., 2015a). In CD-1 mice infected with 10³ plaque forming units of either VEEV or EEEV, both viruses showed similar trends with decreased weight, piloerection, paralysis, seizure, and 100% mortality by day 7 (Gardner et al., 2008). Disease symptoms of VEEV onset earlier with mice surviving longer with clinical signs of CNS infection; whereas EEEV-infected mice showed later onset of symptoms and mortality within 12–24 h post disease onset (Gardner et al., 2008). Potentially, this is driven by cellular tropism differences between VEEV and EEEV, as EEEV replicates poorly in lymphoid tissues while VEEV flourishes in macrophages and dendritic cells in these tissues (Gardner et al., 2008). The overarching similarity between VEEV and EEEV infections in mice are encephalitis; however, in VEEV-infected mice, neurons in the hippocampus and cerebellum show signs of morphological changes correlated with apoptosis, whereas in EEEV seem to infiltrate the thalamus, pons, and putamen with widespread neuronal necrosis (Vogel et al., 2005). There are a few consistent differences noted with VEEV and EEEV infection in mice which has not been fully elucidated in NHP and humans where paralysis is more common in VEEV and have a rarer occurrence of seizures, while with EEEV there are few cases of paralysis and frequent report of seizures (Gardner et al., 2008). Collectively, EEEV pathogenesis and neuroinvasion appear to be more rapid, although EEEV infected mice display widespread neurological damage and proinflammatory activation similar to VEEV with slight alterations dependent on the route of exposure.

2.1.3 WEEV

Several hundred cases of WEEV infection have been well documented, with neurological sequelae lingering for several years, if not lifelong, post-infection. In children and some adults, mild sequelae include loss of taste, changes in speech, decreased fine motor skills, changes in gait, and hearing deficits (Table 2) (Mulder et al., 1951). Severe sequelae in both adults and children include personality and behavioral changes, intellectual disability, seizure, limb weakness, mood swings, depression, anxiety and paranoia (Palmer and Finley, 1956; Fulton and Burton, 1953; Deaton et al., 1986). Neurological manifestation of WEEV infections are the most significantly documented partially attributed to its lower mortality (< 7%) and high incidence of neurological symptoms (15%−30%) (Luethy, 2023; Simon et al., 2023). Documentation of WEEV in an aerosol laboratory-acquired infection lead to the death of 2/5 infected individuals following symptoms such as headache, fever, tachycardia, and increased heart rate (Hanson et al., 1967). A recent outbreak of WEEV occurred in early 2024, where >100 equine cases, most of them fatal, and >100 human cases were documented with 10 fatalities (Campos et al., 2024). Campos et al. (2024) identified a novel WEEV lineage likely due to unreported cases circulating in South America, but it does not currently appear to be a recombination with other EEVs. In this outbreak, most cases were mild or asymptomatic, with signs or meningitis and encephalitis in fatal cases (Campos et al., 2024). Interestingly, some patients with WEEV have been described to have other neurological symptoms that mirror those diagnosed with diseases such as Parkinson's and Schizophrenia (Bantle et al., 2019; Levine and Griffin, 1992; Herzon et al., 1957). One recent case occurred in November 2023 of an equine agricultural working in Argentina, where the patient presented with headache, fever, disorientation, confusion, and tiredness, leading to intensive care treatment for 12 days and eventual discharge nearly 30 days after initial symptoms (World Health Organization, 2023). Given both a recent case and severity of disease across many documented cases, it highlights the need for adequate surveillance, diagnosis, and animal models for therapeutic evaluation against WEEV.

WEEV infections in animal models have substantial research in murine, hamster, and NHP models. Studies of WEEV in NHPs have been minimal since the 1930s. Fever and increased heart rate, as well as clinical signs suggesting encephalitis, were observed in NHPs exposed to WEEV via aerosol (Smith et al., 2020; Reed et al., 2005). Some conflicting reports indicate minimal clinical signs and no recoverable virus from WEEV infected NHPs, but potentially this is due to quicker clearance compared to the other EEVs (Burke et al., 2022). Pathology from infected NHPs has indicated viral infection broadly across the brain with widespread infiltration in the gray matter of the brain in areas of the striatum and cerebrum and infection of the neurons in the hippocampus (Reed et al., 2005). Some models of WEEV and VEEV in NHPs suggest peak neutrophil and monocyte infiltration is slightly later around 7–9 DPI. Despite several studies that have investigated WEEV neuropathology, there is still much left unknown about the course of disease in NHPs, highlighting a significant gap in the literature.

Golden hamsters infected with WEEV resulted in 100% mortality with symptoms such as severe respiratory challenges, blurred cornea, eye discharge, and seizure with confirmation of the virus in the brain within 24 h of infection (Zlotnik et al., 1972). Older studies have revealed neuronal necrosis, edema, glial nodes, perivascular cuffs, and astrocytosis in mice 4–8 weeks old with 100% mortality in 2-day-old mice due to severe inflammation and necrosis of muscle, cartilage and bone marrows prior to neuroinvasion (Aguilar et al., 2011; Phillips et al., 2016; Gardner et al., 2022; Phelps et al., 2017). Suppression of WEEV replication in CD-1 mice using immunotherapy enabled the survival of mice and analysis of neurological sequelae (Bantle et al., 2019). These mice displayed Parkinson's-like symptoms and pathology seen in humans, including a loss of dopaminergic neurons, increased protein aggregation, and persistent neuroinflammatory responses (Bantle et al., 2019). In this model, WEEV replication was confirmed in the olfactory bulb, the cortex, hippocampus, and basal midbrain by day 4 post-infection with prolonged inflammatory response and glia and astrocyte activation 2 months post-infection. A variety of new literature has come out regarding WEEV pathology, especially surrounding its similarities to Parkinson's disease and the chronic consequences of neuroinvasion. Microgliosis, astrogliosis, dopaminergic neuron loss, and a-synuclein protein aggregation are observed across the brain with particular accumulation in the hippocampus and cortex (Bantle et al., 2021).

Despite vast advances in our understanding of disease induced by EEV family members, there have been no studies that have been able to correlate transcriptomic and histopathological features with specific changes in physical, emotional, and behavioral changes seen in humans. Collectively, given the vast similarities indicated in the acute phase of infection, it's likely that therapies could enhance protection against EEV fatality or neurological sequelae.

2.2 TBI

The National Institute of Neurological Disorders and Stroke classifies TBI as an external mechanical force that causes damage to the brain, potentially leading to temporary or permanent disability (NINDS, 2024). The most recent data suggests that over 200,000 TBI related hospitalizations occurred in 2020 and almost 70,000 TBI related deaths occurred in 2021 (CDC, 2024). TBI injury severity varies greatly both across human patients requiring varied animal models to replicate the disease processes and outcomes seen in people. TBI severity can be stratified by the Glasgow Coma Scale (GCS) into mild, moderate, and severe injuries (Vella et al., 2017; Jain and Iverson, 2023). Much of the human research conducted focuses on sports related injuries or combat related injuries (Fehily and Fitzgerald, 2017; Kim et al., 2023; Elder and Cristian, 2009). Even so, the majority of TBIs are caused by falls and frequently impact elderly individuals and young children (Faul et al., 2010). Further, males are over three times more likely to sustain a TBI than females, likely due to more prevalent high-risk behaviors and higher-risk jobs as this sex difference is only present in adults, not children (Alexis et al., 2022). The TBI injury cascade can be generally split into two categories: primary and secondary injury (Davis, 2000). The primary injury reflects the initial mechanical insult, such as axonal shearing, contusion, laceration, and skull fracture. The secondary injury includes the physiological aftermath that causes continued cell damage and death in the hours, days, and years following the initial impact. This includes BBB disruption, hypotension, hyperglycemia, hypoglycemia, changes in intracranial pressure, cerebral edema, peripheral immune cell infiltration, gliosis, and release of excitatory neurotransmitters. These mechanisms lead to the symptoms and outcomes seen in patients and replicated in animal models (Table 3). To replicate human TBI cases, animal models of TBI are adapted to study various injury types and severities (Xiong et al., 2013).

Table 3

Table 3. Comparison of human TBI cases and laboratory animal models of TBI.

Animal models utilized for TBI research include rats, mice, pigs, rabbits, dogs, swine, sheep, ferrets, monkeys, and cats, which have been evaluated and critiqued by others (Xiong et al., 2013; Cernak, 2005; Ma et al., 2019). Non-human primate models of TBI are essential for accurate modeling of TBI impact on neural damage as these models most closely resemble the human brain; however, these models are limited (Barbay et al., 2021). The most common model of TBI is the rodent (mouse and rat), which we will focus on in this review. Additionally, it is essential to consider that TBI is a heterogeneous injury and that there are many injury models that replicate various pathologies, neurological manifestations, and severity levels. While the classification of TBI is under current refinement including the use of endophenotypes, we aim to review several known severity categories in humans (mild, moderate/severe) and compare human disease pathology to rodent models.

2.2.1 Mild TBI

Mild TBI is considered a score of 13–15 on the GCS. Mild TBI is the most common type of TBI, with 70%−90% of treated brain injuries falling into the mild category (Cassidy et al., 2004). Furthermore, it is estimated that the incidence of treated mild TBI is 100–300/100,000, however, the true incidence is likely much higher since many mild TBIs are not treated at a hospital (Cassidy et al., 2004). Risk factors for mild TBI include intoxication, low education, age, intimate partner violence, military deployment, contact sports, and socioeconomic status (Alexis et al., 2022; Nordstrom et al., 2013; Gardner and Yaffe, 2015). Frequently, mild TBIs show no abnormalities on CT or MRI scans, yet many patients suffer from a plethora of symptoms. Common symptoms of a mild TBI include post traumatic amnesia, loss of mental alertness, anterograde amnesia, confusion, speech and gait abnormalities, personality changes, a lack of energy, and sometimes a loss of consciousness. Various rodent models of mild TBI, including controlled cortical impact (CCI), weight drop, closed head injury, and fluid percussion injury (FPI) models display many of the outcomes seen in humans (Table 3) (Bodnar et al., 2019). These symptoms included diminished cognitive and motor capabilities, including deficits in spatial learning and memory, increased anxiety-like behavior, and risk-taking behavior. Further, many of the pathological changes in human cases were replicated in mouse models. Pathological changes include accumulation of phosphorylated tau, white matter structure abnormalities, diffuse axonal injury, inflammation, and BBB disruption (Xu et al., 2021; Wu et al., 2020).

2.2.2 Moderate and severe TBI

Moderate and severe TBIs are classified by having GCS scores of 9–12 and 3–8, respectively. Falls are a leading cause of moderate and severe TBI, particularly for older adults (Iaccarino et al., 2018). Motor vehicle accidents, assaults, and firearm-related injuries also account for many moderate and severe TBIs (Iaccarino et al., 2018; Miller et al., 2020). Loss of consciousness is typically longer with moderate and severe TBI and can last between 30 min and 6 h for moderate TBI and even over 6 h for severe TBI. Common symptomology includes general cognitive impairment, dizziness, confusion, seeing stars, no memory of the injury, persistent or worsening headache, vomiting, nausea, seizures, dilations of pupils, fluid draining from the nose or ears, and/or inability to wake from sleep. Pathological findings can include subarachnoid hemorrhage, subdural hematoma, extradural hematoma, intraventricular hemorrhage, effacement of ventricles, cerebral edema, bradycardia, elevated blood pressure and intracranial pressure, midline shift, cerebral contusion, skull fracture, cerebellar damage, BBB disruption, hippocampal volume loss, increased serum cytokine levels, elevated Glial fibrillary acidic protein (GFAP), myelin basic protein (MBP), and neurofilament light (NfL) deposition. Long-term neurological and psychiatric consequences can include general cognitive impairment, schizophrenia, depression, hallucinations, anxiety, substance abuse, somatoform disorder, adjustment disorder, affective disorder, and general psychiatric diagnosis. Numerous rodent models of moderate and severe TBI have recapitulated human TBI pathology and behavioral deficits (Table 3) (Yu et al., 2009; Pischiutta et al., 2018; Mao et al., 2020).

2.3 NA

Early descriptions of human exposure to NAs occurred through laboratory exposure through inhalation of tabun (ethyl N,N-dimethylphosphoramidocyanidate) (Lopez-Munoz et al., 2008). Exposure to sarin (isopropyl methylphosphonofluoridate), soman (pinacolyl methylphosphonofluoridate), and VX are the most well documented in both patients and animal models (Moshiri et al., 2012). Unlike the aforementioned viruses or TBI models, the mechanism of action and the manifestation of disease is nearly identical across different NAs. The primary mechanism of action of all NAs is the inhibition of acetylcholinesterase activity, leading to accumulation of acetylcholine at neuronal synapses. The excess acetylcholine, termed cholinergic crisis, causes prolonged activation of nicotinic and muscarinic receptor activity, which induces neurotoxic symptoms including muscle cramping, paralysis, headaches, and more (Lopez-Munoz et al., 2008). The accumulation of acetylcholine at the synapses in both the peripheral nervous system (PNS) and CNS leads to widespread CNS damage, with damage in the limbic system, particularly the amygdala and hippocampus, being most noted in the literature (Prager et al., 2014; Shih et al., 2003; Miller et al., 2015). The dentate gyrus of the hippocampus is built of tightly packed cholinergic neurons, therefore, they are highly sensitive to acetylcholine accumulation. Communication between the amygdala and the hippocampus is essential for memory and anxiety behaviors, and these cholinergic neurons play a large role in signal transmission between these portions of the brain (Song, 2023); therefore, it is unsurprising that these regions are significantly impacted by nerve agents (Aroniadou-Anderjaska et al., 2009). While the action of all NAs is similar, the primary differences in disease manifestation are related to the concentration and duration of exposure (CDC, 2017). Unfortunately, high or prolonged low-dosage exposure is highly fatal without immediate medical intervention to mediate seizure damage. The G-series agents (soman and sarin mostly discussed here) are water-like in consistency and easily form vapors, while the V-series agents (VX primarily discussed here) which are a thicker consistency and typically do not vaporize, leading to prolonged presence in the environment (Fan et al., 2024). While not discussed here, it's worth noting that several research articles have used surrogates, which are nonvolatile chemical compounds that inhibit acetylcholinesterase activity but are not as toxic or as strictly controlled as NAs (Finnegan et al., 2021). These compounds are valuable for enabling more laboratory research for therapeutics and biomarker analysis, but are not as toxic as G and V series nerve agents, so they are not the focus of this review.

In the following sections, we will review the animal models of NA exposure, which consist of NHP, rat, and mouse models. Animal models have been reviewed previously (Figueiredo et al., 2018; Pereira et al., 2014). As briefly discussed in future sections, an NHP model is ideal for nerve agent research because humans and primates have conserved nicotinic acetylcholine receptors (Kendrick et al., 2021). Cynomolgus, African green, and rhesus monkeys, as well as common marmosets have been investigated (Despain et al., 2007). Some models that have not been as well evaluated, such as the baboon model, have shown significant airway decline and neuromuscular junction activity, but the effects were inconsistent due to anesthetization with phenobarbital which inhibits some of the clinical presenting signs of NA toxicity, including muscle fasciculation and seizures (Anzueto et al., 1990). Collectively, it has been suggested that NHPs are the best model for NA intoxication due to similar levels of organophosphorus metabolizing carboxylesterases to humans though new humanized mouse models are being developed to better mimic human responses and reduce some of the ethical challenges of NHP experimentation (Marrero-Rosado et al., 2021; Tressler et al., 2024).

2.3.1 G-series nerve agents (GA, GB, GD)

Several instances of G-series NA exposure have been documented in humans, but the extent of exposure and resulting neurological sequelae are not well characterized. Notable use occurred in Northern Iraq (Iraq, Iran War), Syria, and Damascus (Balali-Mood and Saber, 2012; Rosman et al., 2014; Thiermann et al., 1999). Documentation of sarin exposure in humans has been primarily on accidental exposures (Duffy et al., 1979) though several long-term studies of sarin exposure have been conducted on victims of the Tokyo and Matsumoto subway terrorist attacks (Duffy et al., 1979; Okumura et al., 1996). Inhalation and ingestion are the most toxic routes of NA exposure and induce faster, more severe symptoms than dermal contact which can take hours for symptoms to onset (Vucinic et al., 2017). Sarin, tabun, and soman are highly volatile and lethal doses are estimated to be between 10–500 mg-min/m³ (Table 4). Acute symptoms of exposure include headache, nausea, vomiting, and seizures (CDC, 2018). Prolonged symptoms of G-series exposure include depression, anxiety, mood swings, memory impairment, and cognitive and behavioral changes (Figueiredo et al., 2018; Levin and Rodnitzky, 1976; Rosenstock et al., 1991; Wesseling et al., 2002; Savage et al., 1988; Roldan-Tapia et al., 2005). Some evidence has suggested that Gulf War illness, characterized by broadly defined features such as tiredness, pain, memory impairment and imbalance in >250,000 warfighters, may be partially due to low levels of sarin exposure (Haley et al., 2022; Elhaj and Reynolds, 2023). These long-term effects are currently hypothesized to be due to intracellular cytotoxicity that correlates with the white matter edema observations in human cases following the Tokyo sarin attacks, but post mortem analysis of NA-exposed humans is limited (Bhagat et al., 2001, 2005). Collectively, early detection and antiseizure administration are the most crucial mediator of severe OP neurotoxicity (Gupta, 2020).

Table 4

Table 4. Comparison of human cases and laboratory animal models of NA.

Soman exposure in NHPs has been well-established in several models (rhesus, cynomolgus, baboons, African green) to induce tremors, seizures, and muscle spasms (Despain et al., 2007; Raveh et al., 1997; Woodard et al., 1994; Maxwell et al., 1992). Immediate symptoms include chewing, increased salivation, and facial spasms with symptoms progressing to limb twitching, persistent tremors, convulsion, seizure, thrashing, and slumped posture (Despain et al., 2007). Without medical countermeasures to mediate seizure and acute toxicity, NA exposure is typically lethal. The lethal dose reported in African green monkeys was ~7.15 μg/kg, which is consistent with ranges from 5–15 μg/kg in rhesus, cynomolgus, and baboons (Table 4). Seizure activity is closely correlated with administered dosage of soman, where lower dosages result in less severe seizures and other neuromuscular changes, while higher dosage animals are prone to more frequent and longer lasting seizures, apnea, and cyanosis (Despain et al., 2007). Apnea, cardiac arrhythmia, and decreased blood pressure following soman exposure have been most well characterized in NHPs (Despain et al., 2007; Raveh et al., 1997; Woodard et al., 1994; Maxwell et al., 1992). The prolonged activation of acetylcholine receptors in humans and NHPs induces fatal neurotoxicity and, ultimately, death.

Symptoms of nerve agent poisoning in rodents resemble that of humans and NHPs with respiratory challenges, involuntary secretions, chewing, salivation, diarrhea, muscle fasciculation, tremors, convulsions, and seizures (Table 4). Soman can cross the BBB rapidly leading to increases in acetylcholine followed later by glutamate and subsequent neurotoxicity (Bhagat et al., 2005; Shih and McDonough, 1997). A single high dose of sarin or soman induces neuronal loss in rats dependent on seizure activity (McLeod et al., 1984; Petras, 1994). Pathological signs of injury occur soon after injury (4 h) but appear to worsen over time with notably increased severity at 3+ months post injury in the hippocampus, piriform cortex, and thalamus (Kadar et al., 1995). Single doses of sarin induce edema widespread \throughout the brain (Testylier et al., 1999). Extensive pathology of soman-exposed rats with memory deficits showed significant loss of neurons and interneurons and an increase in activated astrocytes and microglia in the hippocampus 90 days post-exposure (Reddy et al., 2020; Marrero-Rosado et al., 2018). BBB disruption and permeability significantly increase with exposure to NA across the brain; however, these impacts appear to be acute while the dysregulation of acetylcholine and muscarinic receptors appears to persist beyond 90 days post-injury. Persistent BBB disruption following NA exposure has not been well documented. Currently, BBB disruption appears to be at its height in the acute phase post-exposure and may be associated with convulsions and seizure typically seen acutely following exposure (Gupta, 2020). However, BBB restoration following injury in general can take time and depends on several factors, including severity of disruption and neuroinflammation. NA exposure is likely not an exception to this. In addition to pathological markers, there has been significant evaluation of neurotoxicity in rodent brains at various timepoints post exposure that display increased cytokine production, oxidative stress, and locomotor activity which becomes more apparent with increasing concentration of nerve agent (Henderson et al., 2002; Abu-Qare and Abou-Donia, 2002; Nieminen et al., 1990; Johnson and Kan, 2010). A review of animal models exposed to soman highlighted that in both rat, guinea pig, and nonhuman primate NA exposure models, only animals with seizures developed neuropathology and these seizures are the best variable for predicting neuron loss (Abdollahi and Karami-Mohajeri, 2012; Jett et al., 2020).

Unique challenges of NA exposure model development are the differing baseline levels of cholinesterase activity between inbred and outbred rodent strains. It has been relatively well established that C57BL/6 mice and C3H/He have less acetylcholinesterase activity in comparison to DBA/2 and BALB/c mice (Atalayer and Rowland, 2010). Genetic differences, including cholinesterase levels, appear to contribute to NA toxicity, especially regarding lethal dose and percent mortality (Matson et al., 2018; Furman et al., 2014). An additional challenge is the presence of serum carboxylesterase activity in rodent models of NAs. These compounds aid in degradation of NAs, thereby reducing the total NA concentration, but this activity appears to be absent in human cases. Differences in rodent response to NAs could also be sex dependent, as neurodegeneration and gliosis 4 months post exposure to soman is more significant in female animals than male animals (Gage et al., 2021).

2.3.2 V-series nerve agents (VE, VG, VM, VR, VX)

The largest evidence of VX exposure consists of nearly 100 warfighters presenting ill-defined symptoms, including “altered awareness,” reduced intellectual ability, slowed movements, anxiety, and confusion (Bowers et al., 1964). V- series NAs induce a variety of symptoms including seizure, salivation, urination, vomiting, spasms, muscle twitching, and tremors (Hayoun et al., 2024). Incidence of seizure is lower in both humans and animals exposed to VX or VR compared to the G-series agents (Shih et al., 2003; Fawcett et al., 2009). Similar to the other OPs, chronic evaluation of nerve agents in humans is limited, but amnesia and behavioral changes may persist years after exposure (Nozaki et al., 1995). While human cell culture models of NAs are limited, a unique study performed a microarray analysis of human neurons and astrocytes exposed to VX and soman, respectively, and identified significant upregulation of apoptosis and inflammatory cascades independent of in vivo hallmarks of seizures and acetylcholine dysregulation (Hoard-Fruchey et al., 2020). Gene expression changes of astrocytes were not dependent on the agent used, but neurons appear to be significantly separated with both datasets showing significant upregulation of genes associated with the inflammatory response. It should be noted though that cell death was not induced by VX or soman in either cell population.

Multiple models for VX exposure exist including goats, guinea pigs, swine and mice. Observations in these animals are consistent with dose-dependent impacts of G-series agents, where facial twitches, chewing, tremors, seizures, but appear to be brief and all animals typically survive (Fawcett et al., 2009; Langston and Myers, 2016; O'Donnell et al., 2011). Ultimately, NHP and guinea pig models, previously reviewed (Pereira et al., 2014), appear to be the most reliable over rat and mouse models. Of the rodent models of VX exposure, there were minor changes in acute toxicity, histopathology, and weight, leading to authors conclusion of unreliable results with subcutaneous administration of sublethal doses (Goldman et al., 1988; Atchison et al., 2001). Minimal exploration of chronic aspects of VX exposure have been explored.

3 Comparative neuropathology

Indicated throughout this review, there are overarching similarities across the three neuropathologies in terms of symptoms and pathology. In humans, the symptomatic manifestations of disease often include seizure, confusion, behavioral or emotional changes. In following sections, we will compare disease progression of EEVs, TBI, and NA.

3.1 Overlapping clinical sequelae in humans

Disease manifestations of EEVs, TBI, and NA include overlapping symptoms and neuropathologies, which highlight the potential for both research gaps in neurological disease mechanisms as well as therapeutic potentials. These illnesses induce mild symptoms such as fever, confusion, headache, shock, neck pain, vomiting, malaise, and chills and can progress to more severe symptoms such as stupor, left-sided weakness, thalamic enhancement, dysarthria, convulsions, seizures, paralysis, intellectual disability, as well as cognitive, motor, and behavioral changes (Figure 1). EEV infection and TBI have a few shared neurological sequelae, including intellectual disability, memory loss, and depression, while convulsions are shared amongst EEV infection and NA exposure. Neurological sequelae shared by TBI and NA exposure include loss of consciousness, slurred speech, partial or complete vision loss, fatigue, insomnia, and drowsiness. All three pathologies can result in paralysis, coma, muscle twitch, photophobia, sleep disorders, and seizures (Figure 1). Although these conditions impact a varying number of individuals worldwide, there are significant case fatalities and progression to neurological sequelae warrantying further evaluation (Table 1).

3.2 General immune response to injury and agent exposure

Immune system activation induced by NA, TBI, and EEVs follow relatively similar patterns; however, the timescale of activation based on the progression of the disease state varies drastically (Figure 2). In brief, primary acute damage driven by NAs occurs within the first 24 h and is primarily driven by immediate neurotransmitter dysregulation and seizures. Continued seizures after the initial injury can occur for days, months, or indefinitely if not mediated by antiseizure therapies. For TBI, the initial damage response recognition initiates a cytokine and chemokine response and glial cell activation in the first 4–5 days, but secondary damage can occur days and months after injury due to prolonged microglia activation and inflammation. EEVs initially evade the immune system and establish primary infection in lymphocytes in the first 4 days of infection, but as the virus spreads to the brain, there is an increase in immune mediators in both the PNS and CNS. While some is known about the immune response to NA and EEVs, much more is known about the immune response to TBI in both human and animal models.

Figure 2

Figure 2. General immune response to injury and agent exposure. (A) Venezuelan equine encephalitis virus acute disease progression is biphasic. (B) Traumatic Brain injury damage consists of two phases: direct injury and secondary tissue damage. (C) Nerve agent damage is driven by neurotransmitter dysregulation. Created in BioRender. Kehn-hall (2024) BioRender.com/i57t162.

While the immune response to EEVs has not been entirely elucidated, the replication and acute response to infection with VEEV have been explored (Skidmore and Bradfute, 2023). VEEV enters the cell via cell surface receptors, passes through the plasma membrane through endocytosis, and replicates within the host cytoplasm before genetic material is encapsulated and buds from the cell (Lundberg et al., 2017). All EEVs have a capsid protein which plays a significant role in dampening the host immune and antiviral responses to promote viral replication (Lundberg et al., 2017). Antivirals aimed at disrupting this activity of VEEV capsid have been tested in vitro (Thomas et al., 2018; Lundberg et al., 2017, 2018; Shechter et al., 2017; DeBono et al., 2019), but no data in animal models is available to date. Efficient replication strategies promote rapid spread into both PNS and established CNS infection, including in the brain within 24–72 h post-infection, depending on the route of infection (Phillips et al., 2016; Salimi et al., 2020) (Figure 2A). Both Type I and II interferon systems are likely initially upregulated by infection, but VEEV has been shown to disrupt nuclear localization of STAT1 and, therefore, reduce Type I and II Interferon systems. Treatment with IFNβ in both rats and NHPs significantly reduced VEEV levels in the CNS post-infection (Cwiklinska et al., 2020; Thorne et al., 2008).

VEEV infection in CH3 mice dampens cytokine levels at 1 DPI, whereas BALB/c mice have a slight upregulation at 1DPI and both models have significant upregulation of chemokines, cytokines, and upregulation of genes associated with interferon responsive genes by 6 DPI (Phelps et al., 2023). Five to six DPI, VEEV-infected mice also display decreased leukocytes and increased T-cells, monocytes, and neutrophils (Phelps et al., 2023). In addition to these gene expression studies, there have been some studies that have evaluated immune responses in mice in different rodent models and with a variety of different VEEV strains. It's proposed that attenuated strains of VEEV induce delayed cytokine expression profiles, but both attenuated and virulent strains of VEEV induce significant upregulation of INF-g, IL-6, IL-12, IL-10, and TNF-a (Grieder et al., 1997). IFNAR-1^−/− and IRF-2^−/− mice show accelerated viral replication, disease onset, and reactive oxygen species, highlighting that these genes play a role in viral dissemination and antiviral response (Schoneboom et al., 2000). Natural killer cells are early innate response immune effectors that induce IFN-y and cell death mechanisms (Taylor et al., 2012). Some reports suggest that natural killer cells also have neuroprotective roles; however, in VEEV, it appears that NK cells increase from 1 to 6 DPI and appear to worsen pathological outcomes of VEEV infection (Segal, 2007). Principal component analysis of a panel of chemokines, cytokines, and other immune and inflammatory markers distinctly separated VEEV-infected animals with pathology, highlighting that VEEV-induced pathology is highly correlated with inflammatory biomarkers (Phelps et al., 2023). Perivascular mononuclear cell infiltration, encephalitis, microglia activation, and neutrophil infiltration in the brain are well established at 7 DPI (Paessler et al., 2006). Collectively, it appears that CNS infiltration appears to occur at relatively the same time as T-cell, B-cell, Natural killer cells, neutrophil, and monocyte upregulation, only slightly later than cytokine and chemokine upregulation, which may promote severe CNS disease.

The time course of the immune response to TBI is biphasic (Figure 2B). Following the initial insult, tissue damage leads to the release of DAMPs (damage-associated molecular patterns). DAMPs stimulate resident cells to release chemokines and cytokines, which in turn recruit neutrophils to the injury site to contain the injury and remove debris. As the neutrophil population dwindles, monocytes begin to infiltrate, and glia become activated around the injury site to initiate reparative processes. T and B cells may also be recruited at later timepoints (Alam et al., 2020; Blennow et al., 2016; McKee and Lukens, 2016). Acute fever has been observed in one rodent models of TBI from day 1–4 post injury (Verduzco-Mendoza et al., 2023), which correlates with clinical case reports of fever after TBI, but this is inconsistently observed and not well characterized (Thompson et al., 2003). After injury, cytokines and chemokines are released, guiding circulating peripheral immune cells to the injury site. Microglia and astrocytes also become activated in response to injury and peripheral immune cell infiltration and can influence long-term outcomes (Loane and Kumar, 2016; Burda et al., 2016). This secondary injury can include but is not limited to, elevated intracranial pressure, ischemia, excitotoxicity, cell death, swelling, axonal injury, BBB disruption, and inflammation (Patel et al., 2023; Kochanek et al., 2000; Yi and Hazell, 2006).

Immune response after NA exposure is driven by convulsion and seizure damage (Figure 2C). Immediate consequences of nerve agent exposure are the inhibition of acetylcholinesterase, accumulation of acetylcholine at the synapse, and subsequent dysregulation of neurotransmitters GABA and glutamate function that leads to neurotoxicity (Figueiredo et al., 2018). Peripheral cytokine and leukocyte activation occur within 30 min post-seizure onset (de Araujo Furtado et al., 2012; Johnson et al., 2011). Proinflammatory cytokines (IL-1B, IL-6) and necrosis factors (TNF-α) increase as early as 2 h post-exposure to NA and peak between 6 and 24 h, depending on the agent and exposure concentration (Johnson and Kan, 2010; Chapman et al., 2006). Inflammation is accompanied by microglial and astrocyte activation, CNS inflammation, and peak injury within the first 48 h (Zimmer et al., 1997). Long-term functional and structural damage primarily dependent primarily depends on the duration and frequency of seizure post-exposure (Hrvat and Kovarik, 2020).

3.3 Acute vs. chronic neurological manifestations of rodent models

Documentation of chronic pathological changes is limited in humans and NHPs; therefore, to eliminate these gaps, we primarily focus on rodent models. While assessment of acute neuropathologies is crucial for animal model development and evaluation of biomarkers; chronic manifestations of disease are less well-understood.

Throughout this review, it has been highlighted that EEV infection induces prolonged neurological symptoms. Acute infection of VEEV yields neuronal necrosis, lesions in the thalamus and olfactory cortex, and perivascular cuffing and gliosis with severe inflammation in the hippocampus and cortex (Reed et al., 2004; Gleiser et al., 1961; Danes et al., 1973; Victor et al., 1956) (Figure 3 and Table 5). While viral replication is not dependent on BBB breakdown, hemorrhage, lesions, and BBB breakdown are observed in acute infection, which could promote worsened chronic outcomes (Cain et al., 2017; Fongsaran et al., 2024). The overarching similarity between VEEV and EEEV infections in mice is the neuronal damage; however, following infection with VEEV, neurons in the hippocampus and cerebellum show signs of morphological changes correlated with apoptosis, whereas damage following EEEV infection seems to induce widespread necrosis, which is uniformly fatal (Steele and Twenhafel, 2010; Honnold et al., 2015a,b). Pathological evidence of vasculitis, perivascular cuffing, edema, hemorrhage, and widespread neuronal necrosis across the brain has also been observed in a variety of different models (Steele and Twenhafel, 2010). The chronic manifestations of EEVs have only recently been explored, but it is evident that VEEV induces neuronal loss, astrocyte activation, neuromuscular deficits, and fear responses several months post-exposure (Ronca et al., 2016, 2017; Fongsaran et al., 2024).

Figure 3

Figure 3. Neuropathology comparison between EEVs, TBI, and NA. Comparison of acute (<30 days post injury or exposure) and chronic manifestations (>30 days post injury or exposure) of disease in rodent models. DPI, days post injury/infection, See Table 5 for associated literature for each observation. Created in BioRender. Kehn-hall (2024) BioRender.com/b05r121.

Table 5

Table 5. Neuropathology comparison between acute and chronic manifestations of EEV, TBI, and NA in rodents.

Despite varying mortality rates and animal models associated with TBI injury (including ferret, mouse, rat, dog, sheep, swine, monkeys, and more) there are a variety of pathological changes which are consistent across the models, including cortical, hippocampal, thalamic degeneration, neuronal death, glial activation, neuroinflammation, excitotoxicity, and BBB disruption (Blennow et al., 2016; Loane and Faden, 2010; Liu et al., 2010) (Figure 3 and Table 5). Following the primary insult, ‘secondary injury' will begin to occur in the minutes, hours, days, weeks, and years post-injury. This secondary injury can include but is not limited to, elevated intracranial pressure, ischemia, excitotoxicity, cell death, swelling, axonal injury, BBB disruption, and inflammation (Burda et al., 2016; Loane and Faden, 2010; Dewan et al., 2018).

For NAs, the severity of the disease is almost exclusively dependent upon the duration of SE post-exposure (Aroniadou-Anderjaska et al., 2008). NA-induced pathology includes severe damage to the amygdala, the thalamus, and the hippocampus (Aroniadou-Anderjaska et al., 2008) (Figure 3 and Table 5). Neuronal loss, neuronal necrosis, and neuronal lesion abnormalities have also been observed (Redell et al., 2020; Apland et al., 2017). In models where prolonged seizures continue for longer durations without medical intervention, the degree of neurodegeneration-induced mortality is very high; therefore, the majority of studies provide a medical countermeasure against seizures to study sequelae.

Collectively, there are signs of neuroinflammation and neuronal loss across all of these models (Figure 3). Additional shared neuropathologies include gliosis, disruption of the BBB, and hippocampal damage. Perhaps the most striking and consistent deficits between the three conditions are associated with regions of the brain associated with memory, namely the hippocampus. This suggests that there are shared neurological pathways altered by infection or exposure, which medical countermeasures can target to alleviate long-term sequelae.

3.4 Behavioral alterations in response to injury and agent exposure

In human cases, symptoms of EEVs, TBI, and NAs often result in behavioral and emotional changes, but the mechanisms behind these personality alterations induced by injury are relatively unknown. Animal models are crucial for developing a better understanding behind the underlying cause and mechanisms which drive these responses (van der Staay et al., 2009). In animal models, neuron damage as a result of NAs, TBI, and EEVs is well documented across the brain with the most striking impacts in the hippocampus, amygdala and other regions of the limbic system (Williams et al., 2023; Shih et al., 2003; Aroniadou-Anderjaska et al., 2008; Palmer et al., 2016; Atkins, 2011). In recent years, there has been an increased interest in memory and anxiety behaviors in rodents and how this information can better inform therapeutic development, including related to how potential therapeutics impact areas of the brain that drive these interactions. In both rodent and human brains, memory formation occurs through three main steps: encoding, storage, and recall, which primarily occurs between sensory signal input from the cortex, signal transduction through the limbic system, and storage within the hippocampus (Joshi et al., 2019).

Behavioral tests to evaluate memory in rodents include both spatial and recognition memory tests, such as Novel Object Recognition, Morris water maze, y-maze and novel arm y-maze. In sarin-exposed (low dosage) and recovered rats with no visible weight difference or persisting symptoms, there is significant impairment of spatial learning and memory via the Morris water maze test (Shi et al., 2023). Further analysis of these animals showed significantly decreased acetylcholine activity in the mouse hippocampus and decreased dendritic spine density in the hippocampal neurons 21 days post-injury, supporting these behavioral deficits. Gene ontology analysis of differentially expressed genes highlighted significant upregulation of genes associated with neurodegenerative diseases, including Huntington's disease and Alzheimer's disease, and downregulation. On a more chronic timescale of 6 weeks post-injury, adult rats exposed to sarin showed poor spatial memory via Y-Maze and the Morris water maze in both animals presenting clinical signs and asymptomatic animals (Kassa and Vachek, 2002; Kassa et al., 2001; Filliat et al., 1999; Raveh et al., 2002). TBI memory deficits have been well established following mild and moderate TBI (Malkesman et al., 2013). The memory deficits appear to be primarily associated with challenges with working memory [reviewed in Paterno et al. (2017)] and more information could be gleaned through new technology such as opto- and chemo-genetics (Paterno et al., 2017). The primary behavior evaluated in most EEV models is neuromuscular function, not memory, as the biosafety challenges with setting up large maze systems in biocontainment adds several challenges and limitations. Several studies have highlighted there is significant viral invasion and damage to the hippocampus as early as 4 days post-infection (Ronca et al., 2017; Williams et al., 2023). Active avoidance, which evaluates fear memory, has been evaluated for VEEV-infected mice with mixed effects as they discovered a reduced latency between shock and escape compared to uninfected controls, but no differences between shock avoidance (Fongsaran et al., 2024). Other behavioral mazes, or the behavior response following EEV infection has not been explored.

Emotional instability and behavioral changes, such as anxiety, are a common complaint in inpatient cases of TBI, NA, and EEVs. In the brain, anxiety is most often associated with the limbic system, of particular importance is the amygdala, which receives information from the cortices and regulates fear, emotion, and motivation (AbuHasan et al., 2024). These changes have been most thoroughly explored in TBI rodent models. Anxiety behaviors of rodents following TBI have been previously reviewed (Malkesman et al., 2013; Tucker and McCabe, 2021). The majority of tests performed in TBI-inflicted animals report increased anxiety in both rats and mice, in both closed head, CCI, and FPI injuries, but there are some outliers (Tucker and McCabe, 2021; Tucker et al., 2017; Lapinlampi et al., 2020; Das et al., 2019). WEEV infection has often been likened to Parkinson's-like disease, and mice exposed to WEEV display abnormal movement gait and increased run duration, which could be indicative of both motor dysfunction and increased anxiety (Bantle et al., 2019). Unfortunately, there has been no specific investigation of anxiety in rodent models of EEVs, but fear memory tests indicate there is a potential alteration of fear anxiety pathways (Fongsaran et al., 2024). At both acute and chronic timepoints following sarin and soman exposure, surviving rats show increased anxiety-like behavior, assessed via open field and elevated T-maze, at both acute and chronic sarin and soman exposure (Sirkka et al., 1990; Baille et al., 2005; Mamczarz et al., 2010). Recordings of other rodent anxiety-like behaviors such as fleeing, hiding, ridged movement, or other prey responses to predators could provide further insight into anxiety in models where behavioral tests have not been performed (Zhao et al., 2023).

One important consideration for rodent research is the difference in behavioral outcomes in regard to the strain of animals being utilized. For example, sham-injured C57BL/6 mice scored significantly better than FVB/N and 129/SvEMS sham mice in motor function assessments (Fox et al., 1999). FVB/N and 129/SvEMS sham mice were not capable of learning in the Morris water maze or Barnes circular maze tasks. Further, C57BL/6 and BALB/c mice show distinct anxiety-like and depressive-like behaviors, pain perception, motor performance, and learning and memory (Mogil et al., 1999; Lucki et al., 2001; An et al., 2011; Garcia and Esquivel, 2018; Stelfa et al., 2022). These strain differences suggest one should carefully select rodent strains based on the research conducted. Collectively, there is indication based on neuropathology and reports of human clinical cases that there are changes in memory and anxiety which could be evaluated using rodent behavioral models.

4 Targeted therapeutics and neuroprotective countermeasures

4.1 Current treatments for EEVs

Currently, no publicly available therapeutics or antiviral treatments are licensed for use in humans infected with VEEV, EEEV, or WEEV. The WHO report indicates no ongoing clinical trials for vaccines or therapeutics against EEVs; however, a recent review of preclinical therapeutics for VEEV is available (Ogorek and Golden, 2023). Several groups have investigated direct-acting antivirals, vaccines, and supportive treatments for injury or inflammation resulting from EEVs (Thomas et al., 2018; Lundberg et al., 2017, 2018; Shechter et al., 2017; DeBono et al., 2019; Ogorek and Golden, 2023; Jonsson et al., 2019; Panny et al., 2023; Lehman et al., 2022; Saikh et al., 2020; Risner et al., 2019; Gall et al., 2018; Carey et al., 2018, 2020; Barrera et al., 2021; Bakovic et al., 2021; Ahmed et al., 2020). Given this, the current treatments are limited to supportive care, a costly treatment with up to $4.6 million-dollar life-time care reported by an EEV infected patient (Villari et al., 1995). In the United States, a live attenuated version of VEEV (VEEV TC83) has been previously used to vaccinate military personnel and at-risk laboratory workers; however, there has been an estimated <30% of those vaccinated developing flu-like illness and adverse reactions (Tigertt et al., 1962; Sewell, 1995). Immunocompetent species of mice and NHPs display similar tropism and CNS infection with one of the hallmarks of infection and major contributing factors to mortality being neuronal damage. Monoclonal antibodies have been used to protect NHPs from VEEV-induced death if administered 48 h post-infection and show some promise as a potential treatment; however, there were mutations in the virus detected in NHPs treated 24 h post-infection (Burke et al., 2019). Thus far, most medical countermeasures of EEVs have focused on the inhibition of viral replication. Prominent antivirals include quinazolinone compound, CID15997213, which inhibits viral replication of VEEV and WEEV; and a ML336 derivate BGDR-4, which has demonstrated over 90% protection against VEEV and EEEV if administrated within 48 h post-infection; although there are potential concerns with viral resistance which is concentration dependent (Jonsson et al., 2019; Chung et al., 2014). CID15997213 targets the viral nonstructural protein 2 (nsP2) (Chung et al., 2014), which contains protease and helicase activities, and BDGR compounds target nsP4, the viral RNA dependent RNA polymerase (Skidmore et al., 2020). The most recent BDGR derivative developed by this group, BDGR-49, shows 70% and 100% protection from 10x LD50 of EEEV (FL93-939) and VEEV (TrD), respectively (Cao et al., 2023). While there are no direct neuroprotective drug treatments for VEEV, there are treatments that have been investigated to reduce neurological damage in viral infection and neurodegenerative diseases. These include pretreatment or co-treatment at infection with melatonin, which significantly reduces mortality, apoptosis, and oxidative stress (Boga et al., 2012; Montiel et al., 2015). Current treatments are challenged by the short window required for adequate inhibition of the virus as well as the potential for viral mutations to overcome antiviral mechanisms. Other challenges are due to the unique feature of illness where many of the human cases that document long-term neurological deficits have mild flu-like symptoms or no symptoms in the early viral infection period, making it challenging to diagnose and prevent. Given this, there is an urgent need for both characterization of CNS infection and neuroprotective treatment options for acute and chronic illness manifestations.

4.2 Current treatments for TBI

As of this publication, there are no FDA-approved medications for the treatment of TBI (Food and Drug Administration, 2021). However, numerous interventions are used in the clinic to alleviate pain and symptomology. Like many other injuries, the elevation of the head has immediate effects as intracranial pressure is reduced as the cerebral spinal fluid is displaced, and venous outflow is increased (Sattur et al., 2023). Intracranial pressure monitoring using an instrument inside the patient's body is an additional option for those who show substantial neurological compromise but do not require immediate surgical intervention (Shim et al., 2023). Hyperventilation is a strategy used to reduce cerebral blood flow via vasoconstriction (Gouvea Bogossian et al., 2020). This is typically only used in severe cases when acute neurological decline is expected to occur. Antiepileptics are often used during the acute phase. However, there is no evidence that these drugs help to prevent PTE in the long term (Chang et al., 2003). The last resort in managing the injury is putting the patient into a medically induced coma, which reduces the metabolic demand in the brain (Galgano et al., 2017). In the most severe cases, often due to cerebral edema and/or severe bleeding, surgical intervention may be warranted. Surgical intervention typically involves a craniotomy over the brain region of interest, followed by the removal of the hematoma and vessel cauterization (Galgano et al., 2017). Altogether, the lack of FDA-approved medication or therapeutics suggests a need to better understand the underlying pathology.

4.3 Current treatments for NAs

Positive outcomes of NA illness induced by acute exposure are heavily reliant upon quick identification of the exposure and immediate treatment. In cases of high-dosage exposure to NAs, or a delay in the time between exposure and treatment, current treatment methods are inadequate at reducing or preventing long-term neurological deficits. Emerging NA biomarkers are crucial for quick identification of NA exposure which could aid in faster and more target specific treatments (Wang et al., 2022). Current treatments for NA-exposed patients typically consist of anti-seizure agents (i.e. benzodiazepine anticonvulsants such as diazepam and midazolam), atropine (a muscarinic acetylcholine receptor antagonist), and pralidoxime (a.k.a 2-PAM, an acetylcholinesterase reactivator) (Figueiredo et al., 2018; Lallement et al., 2001). Benzodiazepines are well characterized in the treatment of NA-induced seizure and are therefore the anticonvulsant drug of choice, while neurosteroids are only recently being explored (Reddy, 2024). Neurosteroids may be an effective treatment for NA-induced seizure, especially seizure that has become refractory to benzodiazepine treatment. However, more research is needed. Combination treatments such as combining the NMDA antagonist ketamine with anticholinergic medications such as atropine have indicated improved outcomes compared to just atropine alone (Marrero-Rosado et al., 2021). Prophylactic treatments, such as carbamate pyridostigmine bromide, have been explored as protective agents against inhibited cholinesterases (Kassa et al., 2001; Bajgar, 2004; Kassa et al., 2008; Gordon et al., 1978). Emerging anticonvulsant options are also being explored, including the neurosteroid, Ganaxolone, which aids in reactivation of GABA-A receptors (Reddy, 2024). Neurosteroids are a promising alternative to benzodiazepines to mitigate acute and refractory seizures. Collectively, several potential therapeutics focused on acetylcholinesterase inhibition could be utilized to pretreat against nerve agent toxicity (van Helden et al., 2011); however, the approval of these therapies are still in the early stages.

4.4 Neuroprotective therapeutics

Neuroprotective therapies have been broadly defined as therapies that either reduce, prevent, or reverse permanent damage to neuron structure and/or function (Levi and Brimble, 2004; Mallah et al., 2020). Most commonly, neuroprotective agents mediate conditions such as Alzheimer's, Parkinson's, or Ischemia (Rehman et al., 2019). As discussed earlier, there are many therapies in the preclinical evaluation stage and some emerging therapies in later stages of the clinical approval pipeline which may be able to mediate some of the neurological deficits caused by EEV, TBI, and NA damage. Currently, the DrugBank online resource cites 43 drugs which are classified as neuroprotective agents (Knox et al., 2024; Wishart et al., 2018; Law et al., 2014; Knox et al., 2011; Wishart et al., 2006).

One area of potential therapeutic development would be guided toward novel treatments selective for modulating neurotransmitter activity to improve neuron signaling. Acetylcholinesterase therapies have proven beneficial in antipsychotic therapy of schizophrenia, as well as visual hallucinations and dementia in Parkinson's and Alzheimer's disease (Singh et al., 2012; Bittner et al., 2023). Gacyclidine, an NMDA receptor agonist, is a psychoactive drug that has been evaluated for a variety of neuroprotective mechanisms, including against OPs. One study evaluated GK-11 (Gacyclidine) treatment in NHPs exposed to OP levels at 8x the LD50 and found it protected animals from mortality (Golime et al., 2018). Similarly, there are a variety of neurotransmitter mediating therapies that aid with both negative regulation of signal transduction, such as Ziconotide which mediates chronic pain via calcium channel blockage (McGivern, 2007), and Tenocyclidin which mediates NMDA binding and provides anesthetic and dissociative effects in a variety of different situations including mediation of severe injuries such as spinal cord or brain injury (Radic et al., 2006). Mediation of the toxic effects of neurotransmitter dysregulation could help preserve neuron function and general immune responses to promote clearance of damaged regions of the brain following injury.

Oxidative stress is a driving mechanism behind many progressive neurodegenerative diseases as well as a potential therapeutic avenue against secondary injuries. Mitochondrial disruption is one of the drivers of this damage and has been identified in EEV (Keck et al., 2018), TBI (Hiebert et al., 2015), and NAs (Pearson and Patel, 2016). Antioxidants can help with scavenging and clearing radical oxygen species in the brain, as well as promoting the stability of naturally occurring antioxidants during phases of mitochondrial dysregulation (Ashok et al., 2022). Several antioxidants have been evaluated in clinical trials against neurological injuries and appear to be a safe and well-tolerated therapeutic option, these have been previously reviewed (Lee et al., 2020; Kelsey et al., 2010; Lalkovicova and Danielisova, 2016; Teleanu et al., 2019).

Combating the death of healthy cells while clearing damaged or misfunctioning cells is a major challenge for every organism. Neurodegenerative diseases almost always have alterations in programmed cell death (apoptosis), mitochondrial recycling (mitophagy), or cellular recycling (autophagy) mechanisms (Knox et al., 2024). For VEEV it has been shown there is an upregulation in apoptosis and mitophagy which ultimately worsens pathology in response to infection (Baer et al., 2016; Keck et al., 2017). Several cell death pathways are induced by TBI and OPNA and are likely a leading cause of secondary injury (Wu and Lipinski, 2019). While these mechanisms require energetic stress and cause the death of cells, autophagic mechanisms are often beneficial as they promote restoration of cellular function (Liao et al., 2022). Therefore, modulating cell death or recycling mechanisms is a challenge, and short-term administration of potential therapeutics could be an avenue of healthy cell death while also promoting the uncontrolled growth of dysfunctional cells. In both spinal cord injury and TBI, rapamycin treatment reduces neuron death through activation of autophagy and microglia activation (Husain and Byrareddy, 2020; Song et al., 2015; Li et al., 2019). In combination with lithium, rapamycin can provide synergistic effects inducing autophagy to enhance the clearing of protein aggregates associated with aging neurons (Sarkar et al., 2008; Cherra, 2008). Apoptosis, driven by caspase-3 activation, is associated with apoptosis in the CNS as well as several diseases, including TBI, spinal cord injury, and Alzheimer's disease (Khan et al., 2015). Activation of the neuronal-specific inhibitor of the apoptosis (IAP) family has been shown to reduce the loss of hippocampal neurons, which could potentially reduce pathology and memory deficits (Robertson et al., 2000). Further evaluation of apoptosis and autophagy mechanisms is necessary to determine specific time points post-injury where these therapies could be beneficial or harmful to patient outcomes.

While this is not an exhaustive list, these neuroprotective therapies highlight crucial areas for advancement in neuroprotective health. Further studies into the mechanisms behind neurological damage, especially those that target deficits common across multiple neurodegenerative diseases, such as memory loss or anxiety, could provide promising avenues for therapeutics.

5 Comparative neurodegenerative diseases

5.1 Alzheimer's disease

Alzheimer's disease is the most common neurodegenerative disease driven by the accumulation of misfolded proteins. Unlike many other neurodegenerative diseases, the onset of Alzheimer's disease is not typically associated with genetic mutations but rather a combination of environmental and lifestyle (i.e., age and fitness) risk factors (Korczyn and Grinberg, 2024). Typically associated with increasing age, the initial symptoms of Alzheimer's disease are mild memory loss, which typically progresses into more severe memory loss over time, but each individual case has a different rate of disease progression. Therapeutic options for Alzheimer's disease are limited, but therapies currently include antipsychotics and mediators of abnormal neurotransmitter function which contribute to memory loss-induced psychosis (Eassa et al., 2023; Drevets and Rubin, 1989; Wang et al., 2023; Ricci et al., 2021). In recent years, there has been an increasing interest in the relationship between infectious agents and neurodegenerative disease due to a variety of shared factors, including neuron loss, increased cellular damage, and increased inflammation (Piekut et al., 2022). Memory deficits have been noted in clinical cases of NA, TBI, and EEV survivors. A recent study highlighted the similarities between VEEV and Alzheimer's utilizing early onset Alzheimer's animal model (Tg2576) revealed that alphavirus infection can speed up neurodegenerative phenotypes associated with Alzheimer's, including pro-damage cytokines TNF-alpha and IL-1B, fear memory formation, and amyloid beta plaque concentrations (Fongsaran et al., 2024). Similar links have been made between Alzheimer's disease and TBI, as there are signs of amyloid beta accumulation and an increase in the same cytokine profiles; however, it is still unclear what leads to the accumulation of these factors beyond the hypothesis that its driven by vascular dysfunction or ischemic damage from BBB disruption (Ramos-Cejudo et al., 2018). Several reversible acetylcholine esterase inhibitors have been utilized to treat Alzheimer's disease and have been proposed as potential prophylactic therapeutic options for acetylcholinesterase toxicity caused by NA exposure given that they can occupy acetylcholinesterase binding sites, therefore minimizing the effects of NA. One of these therapies, Galantamine, was used to rescue animals from death and provided notable prevention from sarin and soman-induced neurodegeneration (Golime et al., 2018; Albuquerque et al., 2006). Acetylcholinesterase-inhibiting therapies have greatly increased as a result of NA release, which can be used for the management of oxidative stress and neurotransmitter dysregulation caused by Alzheimer's disease through increasing acetylcholinesterase levels in the brain, though to a lesser extent than NAs (Singh et al., 2012). Collectively, the overlapping neuropathologies between Alzheimer's disease and the biological, physical, and chemical injuries discussed here further highlight the need for broad neuroprotective and neuroreparative therapies.

5.2 Parkinson's disease

Parkinson's disease is the second most common neurodegenerative disease and is typically characterized by the reduction of dopaminergic neurons partially attributed to mitochondrial alteration, inflammation, and alpha-synuclein aggregation (Pardo-Moreno et al., 2023). Since its earliest discovery, WEEV infections in humans have been often likened to Parkinson's disease and there is significant dopaminergic neuron loss and a-synuclein protein aggregation (Bantle et al., 2019, 2021). Increased rigidity, tremors, and slowed movement have been observed in patients, and further pathological evaluation identified chronic microglia and astrocyte activation as well as aggregation of alpha-synuclein and neuron death (Bantle et al., 2019, 2021). In WEEV-infected patients, a Parkinson's therapy which inhibits decarboxylases (levodopa and trihexyphenidyl) is effective at reducing clinical disease indicating these drugs may be able to aid with long-term deficits (Oertel and Schulz, 2016). Other Parkinson's related therapies, including Caramiphen, Bupropion, and Scopolamine, have been explored for protection against soman in rats because of their ability to antagonize cholinergic and glutaminergic fluxes (Myhrer et al., 2008, 2013). Ultimately, these therapies reduced cognitive impairment induced by soman seizures, but co-administration of these drugs is typically required prophylactically to see robust results.

5.3 Epilepsy and recurrent seizures

Recurrent seizures and epilepsy impact over 65 million people per year and have a variety of causative conditions (Fordington and Manford, 2020; Kanner and Bicchi, 2022). Benzodiazepines for antiseizure effects are most effective against NA-induced neurological deficits because, as discussed previously, seizures are the main cause of neurological damage. Emerging reversable cholinesterase inhibitor therapies which have been used for Parkinson's therapeutic such as physostigmine, are being evaluated for prophylactic effects against NA-induced damage with some success (Myhrer et al., 2008, 2013). Post-exposure therapies for NA revolve around the immediate administration of antiseizure medications as the standard of care, but these same antiseizure measures have only been minorly explored in other neuropathologies, including TBI and EEV infection. In the clinic, there has been the utilization of anticonvulsant therapy to treat reoccurring seizures post symptom onset from humans infected with EEEV, which promoted survival in three patients, but neurological deficits, including hemiparesis and psychomotor deficits, persisted (Carrera I. et al., 2013). In TBI, prophylactic administration of antiepileptic drugs is effective in controlling seizures post-moderate TBI if provided within 7 days post-injury; however, these therapies are not effective if administered after 7 days post-injury (Chang et al., 2003). The effect of antiepileptic therapies beyond the early acute phase of injury or infection appears to be poorly understood. Collectively, antiseizure therapies appear to be effective if administered prior to, or shortly after TBI, NA, or EEV, but are not useful against chronic symptoms. Although antiepileptic therapies have significantly improved over the last 150 years, these therapies still have reduced effectiveness overtime, which appears to also overlap with treating other recurrent seizure conditions; there are still major challenges with the development of tolerance over time, requiring increased dosage and increased adverse effects (Schmidt, 2009). Given these challenges, the development of robust and target selective anticonvulsive therapies are crucial for a large number of seizure-causing diseases.

5.4 Mood, sleep, and behavioral disorders

Mood disorders are one of the most common disabilities worldwide, of which depression and sleep disorders are 18% (Dulawa and Janowsky, 2019) and 20% of the population, respectively (Chattu et al., 2018). Insomnia, nervousness, depression, anxiety, and irritability are common among many neurological diseases including many of the ones listed previously, and EEV, TBI, and NA. Depressive disorders has become known as the most common comorbidity, accompanying neurological disorders as well as cardiovascular, inflammatory, and metabolic disorders (Kochanek et al., 2020). Few mechanisms are known for the major drivers of these behaviors, but there are several categories of depression with different causes, whether that be prior injury, genetic susceptibility, or environmental changes (Remes et al., 2021). Given this, it is not surprising that there are many anti-depressant therapies available, with 5 major types based on mechanisms of action. The most common typically mediate neurotransmitters are dopamine, norepinephrine, and serotonin. While there is much variability in the effectiveness of antidepressants and insomnia treatment, there are some areas of promise for elevating long-term symptoms caused by neurological disease. Melatonin has often been evaluated as a potential neuroprotective therapy to mediate oxidative stress and enhance sleep (Chitimus et al., 2020). Treatment with melatonin significantly reduces symptomatic disease, replicating virus in the brain, and mortality in mice infected with VEEV (Boga et al., 2012; Montiel et al., 2015; Ahmad et al., 2023). In TBI studies, melatonin has both anti-inflammatory and antioxidant properties which reduce secondary injury and chronic neurological deficits (Blum et al., 2021). Albeit a different mechanism of action, the steroid hormone 17β-estradiol, E2, has demonstrated neuroprotective qualities, including reducing TBI-altered miRNAs and reducing inflammation and depressive-like behavior in mice (Sell et al., 2019). Although not tested in NAs, melatonin and other steroid treatments can also be effective at reducing apoptosis, reducing inflammation, and controlling reactive oxygen which are protective in peripheral nerve injuries (Uyanikgil et al., 2017). Uses of melatonin as a treatment option for VEEV, TBI, and NA are currently limited to animal models, but the usage of this naturally occurring and affordable therapy in humans could be a valuable asset to mediating chronic damage induced by these conditions.

6 Conclusions and limitations

As discussed, there are a variety of animal models utilized to assess neurological changes induced by EEVs, TBI and NAs. One confounding variable presented throughout this review is the variation in animal models used between the models due to considerable cost differences and biocontainment challenges. In NHPs, symptoms including seizures, depression, and weight loss have been observed across TBI, NA, and EEVs, but pathology is limited. We can glean the most information from NHP models as these are the most similar to clinical outcomes in humans; however, the majority of the known information about these conditions comes from rodent models of infection.

Much of the research for EEVs has been performed in vitro in human or mouse cell lines and in vivo mouse models, whereas TBI and NA have in-depth in vivo rodent models which are more physiologically similar to humans and show more consistent and reliable results in behavioral tests. In rodent models, symptoms include weight loss, fatigue, and seizures. Pathological changes in the brain, including neuronal necrosis, astrocyte activation, inflammation, and blood-brain barrier disruption, are also common among these conditions in many areas, including those that mediate memory and anxiety.

While NHP models appear to be most similar to the human course of disease in TBI, NA, and EEVs, there is much more known about the transcriptomic and pathology changes in lower-order models such as mice and rats due to obvious cost and accessibility limitations. Some of the differences observed in NHP models compared to rodent models may be due to differing levels of neurotransmitters that mediate neuron communication and response to stimuli. NHPs have levels similar to those of the neurotransmitter acetylcholine in humans, while rodents typically have higher levels. This is attributed to worsened outcomes in rodent models exposed to NAs; however, it is unknown how levels of acetylcholine contribute to disease outcomes in EEVs.

Sex differences in humans and animal models is another important consideration in the TBI field. In the general population, data shows that men are more likely to sustain TBIs than women. Women report worse and more symptoms post injury, but research involving sex differences is impacted by fewer women than men being recruited or involved in work related injury (i.e. military services, motor vehicle injuries, competitive sports) (Gupte et al., 2019; Munivenkatappa et al., 2016). Within collegiate sports, women sustain more concussions than men who are engaging in the same sport. Further, there has been an increase in the number of women joining active military duty. Hereby, there is an increased risk of sport and combat related TBIs in women (Amoroso and Iverson, 2017; Covassin et al., 2016). The role of sex as a variable has been minorly explored in NA and EEVs, therefore; its beneficial to consider other neurological diseases for insight into how sex may play a role in disease outcome. The current literature suggests females may experience worsened disease in NA exposure (Sekijima et al., 1997). In VEEV exposure, more males have reported infection and a slightly higher percentage of neurological symptoms such as headaches, but it's this difference is not understood nor explored in animal models (Carrera et al., 2018). As discussed, sex as a variable has been briefly explored for TBI where women report worse and more symptoms post-injury, but this is impacted by fewer women than men being recruited or involved in work-related injury (i.e. military services, motor vehicle injuries, competitive sports) (Gupte et al., 2019; Munivenkatappa et al., 2016).

Age is also a confounding variable across the models. It has been well established that children infected with EEVs have significantly higher mortality and chronic behavioral changes compared to adults (Farber et al., 1940). In NA, younger exposed individuals can have more increased mortality and more severe responses due to different physiological changes in cholinergic stress, increased risk of respiratory distress, and reduced effectiveness of atropine requiring higher doses (Figueiredo et al., 2018; Klass and Westmoreland, 1985; Rotenberg and Newmark, 2003). Pediatric cases of TBI are devastating with higher levels of mortality and increased antisocial, depressive, and maladaptive behaviors beyond acute injury (Levin and Hanten, 2005; Andrews et al., 1998). Both age and sex are important variables for consideration of response to injury and therapeutics as there are notable differences in neuroanatomy and responses to pharmaceutical agents (Soldin and Mattison, 2009; McCarthy, 2016).

7 Future perspectives

Recent advances in our understanding of overlapping neurological pathways that drive chronic sequelae, disease outcomes, and associated behavioral changes have highlighted potential routes for interventional neuroprotective therapeutics. Despite having diverse causes, the biological, physical, and chemical injuries described have many similarities to other neurodegenerative diseases. More studies have been conducted on the chronic consequences of TBI due to vast number of individuals impacted by TBI expanding across military and civilian personnel. However, there have been limited studies to characterize the chronic consequences of EEVs and NAs in animal models. This is an area of great need to allow comparison between neurological sequelae observed in humans and in animal models. Recapitulation of neurological sequelae in animal models is critical to enable testing of therapeutics that can prevent neurological sequelae.

Expansion to include comparisons between all three disease/injury states should be considered given the potential of chemical and biological warfare threats. Cross-condition therapeutics which target secondary injury including inflammation, neuron loss, and BBB disruption could be targeted with therapeutics in acute phases of injury to promote survival and reduce long-term neurological deficits. Prophylactic measures could also be highly beneficial for military personnel going into areas of high-risk for physical, biological, or chemical agent exposure. Additional work evaluating the similarities of these therapies could reveal potential therapeutic countermeasures. Albeit not discussed in depth here, future studies which elaborate on integrative omics such as transcriptomic, epigenetic, metabolomic, and proteomic changes could aid in providing a better understanding of why neurological sequelae vary across individuals. Furthermore, there are several therapeutics mentioned in the literature that have promising neuroprotective qualities in rodent models inflicted with one type of injury or infection. Expanding these therapeutics to higher-order animal models or expansion of these therapeutics to other viruses, organophosphates, or physical injury could improve the drug discovery landscape and promote movement through the drug approval process more quickly.

Author contributions

MV: Conceptualization, Writing – original draft, Writing – review & editing. CJ: Writing – original draft, Writing – review & editing. JL: Writing – original draft, Writing – review & editing. HX: Project administration, Supervision, Writing – review & editing. MT: Project administration, Supervision, Writing – review & editing. EJ: Project administration, Supervision, Writing – original draft, Writing – review & editing. KK-H: Conceptualization, Funding acquisition, Project administration, Supervision, Writing – review & editing.

Funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by the Defense Threat Reduction Agency grant HDTRA1-22-1-0009 to KK-H. Funders do not have any role in the design of the study and collection, analysis, and interpretation of data. The content of the information does not necessarily reflect the position or the policy of the federal government, and no official endorsement should be inferred.

Acknowledgments

Any exclusions of literature are attributed to space limitations and an attempt to preserve brevity, not the quality of researchers work. All figures were created in BioRender.com.

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Generative AI statement

The authors declare that no Generative AI was used in the creation of this manuscript.

Publisher's note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

References

Abdel-Rahman, A., Shetty, A. K., and Abou-Donia, M. B. (2002). Acute exposure to sarin increases blood brain barrier permeability and induces neuropathological changes in the rat brain: dose-response relationships. Neuroscience 113, 721–741. doi: 10.1016/S0306-4522(02)00176-8

PubMed Abstract | Crossref Full Text | Google Scholar

Abdollahi, M., and Karami-Mohajeri, S. A. (2012). comprehensive review on experimental and clinical findings in intermediate syndrome caused by organophosphate poisoning. Toxicol. Appl. Pharmacol. 258, 309–314. doi: 10.1016/j.taap.2011.11.014

PubMed Abstract | Crossref Full Text | Google Scholar

Abou-Donia, M. B., Siracuse, B., Gupta, N., and Sobel Sokol, A. (2016). Sarin (GB, O-isopropyl methylphosphonofluoridate) neurotoxicity: critical review. Crit. Rev. Toxicol. 46, 845–875. doi: 10.1080/10408444.2016.1220916

PubMed Abstract | Crossref Full Text | Google Scholar

AbuHasan, Q., Reddy, V., and Siddiqui, W. (2024). Neuroanatomy, Amygdala. Treasure Island, FL: StatPearls.

Google Scholar

Abu-Qare, A. W., and Abou-Donia, M. B. (2002). Sarin: health effects, metabolism, and methods of analysis. Food Chem. Toxicol. 40, 1327–1333. doi: 10.1016/S0278-6915(02)00079-0

PubMed Abstract | Crossref Full Text | Google Scholar

Adeyinka, A., Muco, E., Regina, A. C., and Pierre, L. (2024). Organophosphates. Treasure Island, FL: StatPearls.

Google Scholar

Agoston, D. V., Vink, R., Helmy, A., Risling, M., Nelson, D., Prins, M., et al. (2019). How to translate time: the temporal aspects of rodent and human pathobiological processes in traumatic brain injury. J. Neurotrauma 36, 1724–1737. doi: 10.1089/neu.2018.6261

PubMed Abstract | Crossref Full Text | Google Scholar

Aguilar, P. V., Estrada-Franco, J. G., Navarro-Lopez, R., Ferro, C., Haddow, A. D., Weaver, S. C., et al. (2011). Endemic Venezuelan equine encephalitis in the Americas: hidden under the dengue umbrella. Future Virol. 6, 721–740. doi: 10.2217/fvl.11.50

Crossref Full Text | Google Scholar

Ahmad, M. F., Ahmad, F. A., Alsayegh, A. A., Zeyaullah, M., AlShahrani, A. M., Muzammil, K, et al. (2024). Pesticides impacts on human health and the environment with their mechanisms of action and possible countermeasures. Heliyon 10:e29128. doi: 10.1016/j.heliyon.2024.e29128

PubMed Abstract | Crossref Full Text | Google Scholar

Ahmad, S. B., Ali, A., Bilal, M., Rashid, S. M., Wani, A. B., Bhat, R. R., et al. (2023). Melatonin and health: insights of melatonin action, biological functions, and associated disorders. Cell. Mol. Neurobiol. 43, 2437–2458. doi: 10.1007/s10571-023-01324-w

PubMed Abstract | Crossref Full Text | Google Scholar

Ahmed, A., Bakovic, A., Risner, K., Kortchak, S., Der Torossian Torres, M., de la Fuente-Nunez, C, et al. (2020). Synthetic host defense peptides inhibit venezuelan equine encephalitis virus replication and the associated inflammatory response. Sci. Rep. 10:21491. doi: 10.1038/s41598-020-77990-3

PubMed Abstract | Crossref Full Text | Google Scholar

Ahmed, Z. (2022). Current clinical trials in traumatic brain injury. Brain Sci. 12:527. doi: 10.3390/brainsci12050527

PubMed Abstract | Crossref Full Text | Google Scholar

Alam, A., Thelin, E. P., Tajsic, T., Khan, D. Z., Khellaf, A., Patani, R., et al. (2020). Cellular infiltration in traumatic brain injury. J. Neuroinflammation. 17:328. doi: 10.1186/s12974-020-02005-x

PubMed Abstract | Crossref Full Text | Google Scholar

Albe, J. R., Ma, H., Gilliland, T. H., McMillen, C. M., Gardner, C. L., Boyles, D. A., et al. (2021). Physiological and immunological changes in the brain associated with lethal eastern equine encephalitis virus in macaques. PLoS Pathog. 17:e1009308. doi: 10.1371/journal.ppat.1009308

PubMed Abstract | Crossref Full Text | Google Scholar

Albuquerque, E. X., Pereira, E. F., Aracava, Y., Fawcett, W. P., Oliveira, M., Randall, W. R., et al. (2006). Effective countermeasure against poisoning by organophosphorus insecticides and nerve agents. Proc. Natl. Acad. Sci. USA. 103, 13220–13225. doi: 10.1073/pnas.0605370103

PubMed Abstract | Crossref Full Text | Google Scholar

Alder, J., Fujioka, W., Lifshitz, J., Crockett, D. P., and Thakker-Varia, S. (2011). Lateral fluid percussion: model of traumatic brain injury in mice. J. Vis. Exp. 3063. doi: 10.3791/3063

PubMed Abstract | Crossref Full Text | Google Scholar

Alexis, B., Peterson, P., Karen, E., Thomas, M., and Hong Zhou, M. (2022). MPH2 Surveillance Report of Traumatic Brain Injury-related Deaths by Age Group, Sex, and Mechanism of Injury. Atlanta, GA: Centers for Disease Control and Prevention.

Google Scholar

Alexis, R. J., Jagdish, S., Sukumar, S., Pandit, V. R., Palnivel, C., Antony, M. J., et al. (2018). Clinical profile and autopsy findings in fatal head injuries. J. Emerg. Trauma Shock. 11, 205–210. doi: 10.4103/JETS.JETS_127_17

PubMed Abstract | Crossref Full Text | Google Scholar

Allon, N., Rabinovitz, I., Manistersky, E., Weissman, B. A., and Grauer, E. (2005). Acute and long-lasting cardiac changes following a single whole-body exposure to sarin vapor in rats. Toxicol. Sci. 87, 385–390. doi: 10.1093/toxsci/kfi263

PubMed Abstract | Crossref Full Text | Google Scholar

Amare, A. T., Tesfaye, T. D., Ali, A. S., Woelile, T. A., Birlie, T. A., Kebede, W. M., et al. (2021). Survival status and predictors of mortality among traumatic brain injury patients in an Ethiopian hospital: a retrospective cohort study. Afr. J. Emerg. Med. 11, 396–403. doi: 10.1016/j.afjem.2021.06.003

PubMed Abstract | Crossref Full Text | Google Scholar

Amoroso, T., and Iverson, K. M. (2017). Acknowledging the risk for traumatic brain injury in women veterans. J. Nerv. Ment. Dis. 205, 318–323. doi: 10.1097/NMD.0000000000000621

PubMed Abstract | Crossref Full Text | Google Scholar

An, X. L., Zou, J. X., Wu, R. Y., Yang, Y., Tai, F. D., Zeng, S. Y., et al. (2011). Strain and sex differences in anxiety-like and social behaviors in C57BL/6J and BALB/cJ mice. Exp. Anim. 60, 111–123. doi: 10.1538/expanim.60.111

PubMed Abstract | Crossref Full Text | Google Scholar

Andrews, T. K., Rose, F. D., and Johnson, D. A. (1998). Social and behavioural effects of traumatic brain injury in children. Brain Inj. 12, 133–138. doi: 10.1080/026990598122755

PubMed Abstract | Crossref Full Text | Google Scholar

Anzueto, A., deLemos, R. A., Seidenfeld, J., Moore, G., Hamil, H., Johnson, D., et al. (1990). Acute inhalation toxicity of soman and sarin in baboons. Fundam. Appl. Toxicol. 14, 676–687. doi: 10.1016/0272-0590(90)90293-S

PubMed Abstract | Crossref Full Text | Google Scholar

Apland, J. P., Aroniadou-Anderjaska, V., Figueiredo, T. H., Prager, E. M., Olsen, C. H., Braga, M. F. M., et al. (2017). Susceptibility to soman toxicity and efficacy of LY293558 against soman-induced seizures and neuropathology in 10-month-old male rats. Neurotox. Res. 32, 694–706. doi: 10.1007/s12640-017-9789-7

PubMed Abstract | Crossref Full Text | Google Scholar

Aroniadou-Anderjaska, V., Figueiredo, T. H., Apland, J. P., Prager, E. M., Pidoplichko, V. I., Miller, S. L., et al. (2016). Long-term neuropathological and behavioral impairments after exposure to nerve agents. Ann. N. Y. Acad. Sci. 1374, 17–28. doi: 10.1111/nyas.13028

PubMed Abstract | Crossref Full Text | Google Scholar

Aroniadou-Anderjaska, V., Figueiredo, T. H., Apland, J. P., Qashu, F., and Braga, M. F. (2009). Primary brain targets of nerve agents: the role of the amygdala in comparison to the hippocampus. Neurotoxicology 30, 772–776. doi: 10.1016/j.neuro.2009.06.011

PubMed Abstract | Crossref Full Text | Google Scholar

Aroniadou-Anderjaska, V., Figueiredo, T. H., de Araujo Furtado, M., Pidoplichko, V. I., and Braga, M. F. M. (2023). Mechanisms of organophosphate toxicity and the role of acetylcholinesterase inhibition. Toxics 11. doi: 10.3390/toxics11100866

PubMed Abstract | Crossref Full Text | Google Scholar

Aroniadou-Anderjaska, V., Fritsch, B., Qashu, F., and Braga, M. F. (2008). Pathology and pathophysiology of the amygdala in epileptogenesis and epilepsy. Epilepsy Res. 78, 102–116. doi: 10.1016/j.eplepsyres.2007.11.011

PubMed Abstract | Crossref Full Text | Google Scholar

Aronson, J. F., Grieder, F. B., Davis, N. L., Charles, P. C., Knott, T., Brown, K., et al. (2000). A single-site mutant and revertants arising in vivo define early steps in the pathogenesis of Venezuelan equine encephalitis virus. Virology 270, 111–123. doi: 10.1006/viro.2000.0241

PubMed Abstract | Crossref Full Text | Google Scholar

Arrigo, N. C., Adams, A. P., and Weaver, S. C. (2010). Evolutionary patterns of eastern equine encephalitis virus in North versus South America suggest ecological differences and taxonomic revision. J. Virol. 84, 1014–1025. doi: 10.1128/JVI.01586-09

PubMed Abstract | Crossref Full Text | Google Scholar

Ashani, Y., and Catravas, G. N. (1981). Seizure-induced changes in the permeability of the blood-brain barrier following administration of anticholinesterase drugs to rats. Biochem. Pharmacol. 30, 2593–2601. doi: 10.1016/0006-2952(81)90587-6

PubMed Abstract | Crossref Full Text | Google Scholar

Ashok, A., Andrabi, S. S., Mansoor, S., Kuang, Y., Kwon, B. K., Labhasetwar, V., et al. (2022). Antioxidant therapy in oxidative stress-induced neurodegenerative diseases: role of nanoparticle-based drug delivery systems in clinical translation. Antioxidants 11. doi: 10.3390/antiox11020408

PubMed Abstract | Crossref Full Text | Google Scholar

Atalayer, D., and Rowland, N. E. (2010). Comparison of C57BL/6 and DBA/2 mice in food motivation and satiety. Physiol. Behav. 99, 679–683. doi: 10.1016/j.physbeh.2010.02.001

PubMed Abstract | Crossref Full Text | Google Scholar

Atchison, C. R., Holmes, C., Akers, S., and Shih, T.M.-A. (2001). “Guinea pig model for low dose chronic exposure to nerve agent,” in Proceedings of the Scientific Conference on Chemical and Biological Defense Research (The National Academies Press).

Google Scholar

Atkins, C. M. (2011). Decoding hippocampal signaling deficits after traumatic brain injury. Transl. Stroke Res. 2, 546–555. doi: 10.1007/s12975-011-0123-z

PubMed Abstract | Crossref Full Text | Google Scholar

Baer, A., Lundberg, L., Swales, D., Waybright, N., Pinkham, C., Dinman, J. D., et al. (2016). Venezuelan equine encephalitis virus induces apoptosis through the unfolded protein response activation of EGR1. J. Virol. 90, 3558–3572. doi: 10.1128/JVI.02827-15

PubMed Abstract | Crossref Full Text | Google Scholar

Baille, V., Clarke, P. G., Brochier, G., Dorandeu, F., Verna, J. M., Four, E., et al. (2005). Soman-induced convulsions: the neuropathology revisited. Toxicology 215, 1–24. doi: 10.1016/j.tox.2005.05.028

PubMed Abstract | Crossref Full Text | Google Scholar

Baille, V., Dorandeu, F., Carpentier, P., Bizot, J. C., Filliat, P., Four, E., et al. (2001). Acute exposure to a low or mild dose of soman: biochemical, behavioral and histopathological effects. Pharmacol. Biochem. Behav. 69, 561–569. doi: 10.1016/S0091-3057(01)00549-4

PubMed Abstract | Crossref Full Text | Google Scholar

Baille-Le Crom, V., Collombet, J. M., Carpentier, P., Brochier, G., Burckhart, M. F., Foquin, A., et al. (1995). Early regional changes of GFAP mRNA in rat hippocampus and dentate gyrus during soman-induced seizures. Neuroreport 7, 365–369. doi: 10.1097/00001756-199512290-00086

PubMed Abstract | Crossref Full Text | Google Scholar

Bajgar, J. (2004). Organophosphates/nerve agent poisoning: mechanism of action, diagnosis, prophylaxis, and treatment. Adv. Clin. Chem. 38, 151–216. doi: 10.1016/S0065-2423(04)38006-6

PubMed Abstract | Crossref Full Text | Google Scholar

Bakovic, A., Bhalla, N., Alem, F., Campbell, C., Zhou, W., Narayanan, A., et al. (2021). Inhibitors of venezuelan equine encephalitis virus identified based on host interaction partners of viral non-structural protein 3. Viruses 13:1533. doi: 10.3390/v13081533

PubMed Abstract | Crossref Full Text | Google Scholar

Balali-Mood, M., and Saber, H. (2012). Recent advances in the treatment of organophosphorous poisonings. Iran. J. Med. Sci. 37, 74–91.

Google Scholar

Bantle, C. M., Phillips, A. T., Smeyne, R. J., Rocha, S. M., Olson, K. E., Tjalkens, R. B., et al. (2019). Infection with mosquito-borne alphavirus induces selective loss of dopaminergic neurons, neuroinflammation and widespread protein aggregation. NPJ Parkinsons Dis. 5:20. doi: 10.1038/s41531-019-0090-8

PubMed Abstract | Crossref Full Text | Google Scholar

Bantle, C. M., Rocha, S. M., French, C. T., Phillips, A. T., Tran, K., Olson, K. E., et al. (2021). Astrocyte inflammatory signaling mediates alpha-synuclein aggregation and dopaminergic neuronal loss following viral encephalitis. Exp. Neurol. 346:113845. doi: 10.1016/j.expneurol.2021.113845

PubMed Abstract | Crossref Full Text | Google Scholar

Barbay, S., Zhang, H., Frost, S. B., Peterson, J. C., Guggenmos, D. J., Hudson, H. M., et al. (2021). A cortical injury model in a non-human primate to assess execution of reach and grasp actions: implications for recovery after traumatic brain injury. J. Neurosci. Methods. 361:109283. doi: 10.1016/j.jneumeth.2021.109283

PubMed Abstract | Crossref Full Text | Google Scholar

Barrera, M. D., Callahan, V., Akhrymuk, I., Bhalla, N., Zhou, W., Campbell, C., et al. (2021). Proteomic Discovery of VEEV E2-host partner interactions identifies GRP78 inhibitor HA15 as a potential therapeutic for alphavirus infections. Pathogens 10. doi: 10.3390/pathogens10030283

PubMed Abstract | Crossref Full Text | Google Scholar

Bartlett, M. L., Goux, H., Johnson, L., Schully, K. L., Gregory, M., Brandsma, J., et al. (2024). Retrospective analysis of blood biomarkers of neurological injury in human cases of viral infection and bacterial sepsis. J. Infect. Dis jiae445. doi: 10.1101/2024.01.05.24300906

PubMed Abstract | Crossref Full Text | Google Scholar

Baskaya, M. K., Rao, A. M., Dogan, A., Donaldson, D., and Dempsey, R. J. (1997). The biphasic opening of the blood-brain barrier in the cortex and hippocampus after traumatic brain injury in rats. Neurosci. Lett. 226, 33–36. doi: 10.1016/S0304-3940(97)00239-5

PubMed Abstract | Crossref Full Text | Google Scholar

Beamer, M., Tummala, S. R., Gullotti, D., Kopil, C., Gorka, S., Ted, A., et al. (2016). Primary blast injury causes cognitive impairments and hippocampal circuit alterations. Exp. Neurol. 283(Pt A), 16–28. doi: 10.1016/j.expneurol.2016.05.025

PubMed Abstract | Crossref Full Text | Google Scholar

Berge, T. O., Gleiser, C. A., ochenour, W. S. Jr., Miesse, M. L., and Tigertt, W. D. (1961). Studies on the virus of Venezuelan equine encephalomyelitis. II. Modification by specific immune serum of response of central nervous system of mice. J. Immunol. 87, 509–517. doi: 10.4049/jimmunol.87.5.509

PubMed Abstract | Crossref Full Text | Google Scholar

Bhagat, Y. A., Obenaus, A., Hamilton, M. G., and Kendall, E. J. (2001). Magnetic resonance imaging predicts neuropathology from soman-mediated seizures in the rodent. Neuroreport 12, 1481–1487. doi: 10.1097/00001756-200105250-00037

PubMed Abstract | Crossref Full Text | Google Scholar

Bhagat, Y. A., Obenaus, A., Hamilton, M. G., Mikler, J., and Kendall, E. J. (2005). Neuroprotection from soman-induced seizures in the rodent: evaluation with diffusion- and T2-weighted magnetic resonance imaging. Neurotoxicology 26, 1001–1013. doi: 10.1016/j.neuro.2005.04.006

PubMed Abstract | Crossref Full Text | Google Scholar

Bittner, N., Funk, C. S. M., Schmidt, A., Bermpohl, F., Brandl, E. J., Algharably, E. E. A., et al. (2023). Psychiatric adverse events of acetylcholinesterase inhibitors in Alzheimer's disease and Parkinson's dementia: systematic review and meta-analysis. Drugs Aging 40, 953–964. doi: 10.1007/s40266-023-01065-x

PubMed Abstract | Crossref Full Text | Google Scholar

Blennow, K., Brody, D. L., Kochanek, P. M., Levin, H., McKee, A., Ribbers, G. M., et al. (2016). Traumatic brain injuries. Nat Rev Dis Primers. 2, 1–19. doi: 10.1038/nrdp.2016.84

PubMed Abstract | Crossref Full Text | Google Scholar

Blick, D. W., Kerenyi, S. Z., Miller, S., Murphy, M. R., Brown, G. C., Hartgraves, S. L., et al. (1991). Behavioral toxicity of anticholinesterases in primates: chronic pyridostigmine and soman interactions. Pharmacol. Biochem. Behav. 38, 527–532. doi: 10.1016/0091-3057(91)90008-P

PubMed Abstract | Crossref Full Text | Google Scholar

Blum, J. A., Klemm, S., Shadrach, J. L., Guttenplan, K. A., Nakayama, L., Kathiria, A., et al. (2021). Single-cell transcriptomic analysis of the adult mouse spinal cord reveals molecular diversity of autonomic and skeletal motor neurons. Nat. Neurosci. 24, 572–583. doi: 10.1038/s41593-020-00795-0

PubMed Abstract | Crossref Full Text | Google Scholar

Bodnar, C. N., Roberts, K. N., Higgins, E. K., and Bachstetter, A. D. A. (2019). Systematic review of closed head injury models of mild traumatic brain injury in mice and rats. J. Neurotrauma 36, 1683–1706. doi: 10.1089/neu.2018.6127

PubMed Abstract | Crossref Full Text | Google Scholar

Boga, J. A., Coto-Montes, A., Rosales-Corral, S. A., Tan, D. X., and Reiter, R. J. (2012). Beneficial actions of melatonin in the management of viral infections: a new use for this “molecular handyman”? Rev. Med. Virol. 22, 323–338. doi: 10.1002/rmv.1714

PubMed Abstract | Crossref Full Text | Google Scholar

Bolkvadze, T., and Pitkanen, A. (2012). Development of post-traumatic epilepsy after controlled cortical impact and lateral fluid-percussion-induced brain injury in the mouse. J. Neurotrauma 29, 789–812. doi: 10.1089/neu.2011.1954

PubMed Abstract | Crossref Full Text | Google Scholar

Bonanno, M., De Luca, R., De Nunzio, A. M., Quartarone, A., and Calabro, R. S. (2022). Innovative technologies in the neurorehabilitation of traumatic brain injury: a systematic review. Brain Sci. 12:1678. doi: 10.3390/brainsci12121678

PubMed Abstract | Crossref Full Text | Google Scholar

Bowen, G. S., Fashinell, T. R., Dean, P. B., and Gregg, M. B. (1976). Clinical aspects of human Venezuelan equine encephalitis in Texas. Bull. Pan Am. Health Organ. 10, 46–57.

PubMed Abstract | Google Scholar

Bowers, M. B. Jr., Goodman, E., and Sim, V. M. (1964). Some behavioral changes in man following anticholinesterase administration. J Nerv Ment Dis. 138, 383–389. doi: 10.1097/00005053-196404000-00009

PubMed Abstract | Crossref Full Text | Google Scholar

Bowman, K., Matney, C., and Berwick, D. M. (2022). Improving traumatic brain injury care and research: a report from the national academies of sciences, engineering, and medicine. JAMA. 327, 419–420. doi: 10.1001/jama.2022.0089

PubMed Abstract | Crossref Full Text | Google Scholar

Bramwell, E. C. B., Ladell, W. S. S., and Shephard, R. J. (1963). Human Exposure to VX Vapour. Porton Technical Paper no 830. The National Academies Press.

Google Scholar

Brooks, W. M., Stidley, C. A., Petropoulos, H., Jung, R. E., Weers, D. C., Friedman, S. D., et al. (2000). Metabolic and cognitive response to human traumatic brain injury: a quantitative proton magnetic resonance study. J. Neurotrauma 17, 629–640. doi: 10.1089/089771500415382

PubMed Abstract | Crossref Full Text | Google Scholar

Broussard, J. I., Acion, L., De Jesus-Cortes, H., Yin, T., Britt, J. K., Salas, R., et al. (2018). Repeated mild traumatic brain injury produces neuroinflammation, anxiety-like behaviour and impaired spatial memory in mice. Brain Inj. 32, 113–122. doi: 10.1080/02699052.2017.1380228

PubMed Abstract | Crossref Full Text | Google Scholar

Burda, J. E., Bernstein, A. M., and Sofroniew, M. V. (2016). Astrocyte roles in traumatic brain injury. Exp. Neurol. 275(Pt 3), 305–315. doi: 10.1016/j.expneurol.2015.03.020

PubMed Abstract | Crossref Full Text | Google Scholar

Burke, C. W., Erwin-Cohen, R. A., Goodson, A. I., Wilhelmsen, C., Edmundson, J. A., White, C. E., et al. (2022). Efficacy of western, eastern, and venezuelan equine encephalitis (WEVEE) virus-replicon particle (VRP) vaccine against WEEV in a non-human primate animal model. Viruses 14:1502. doi: 10.3390/v14071502

PubMed Abstract | Crossref Full Text | Google Scholar

Burke, C. W., Froude, J. W., Rossi, F., White, C. E., Moyer, C. L., Ennis, J., et al. (2019). Therapeutic monoclonal antibody treatment protects nonhuman primates from severe Venezuelan equine encephalitis virus disease after aerosol exposure. PLoS Pathog. 15:e1008157. doi: 10.1371/journal.ppat.1008157

PubMed Abstract | Crossref Full Text | Google Scholar

Cain, M. D., Klein, N. R., Jiang, X., and Klein, R. S. (2023). Post-exposure intranasal IFNalpha suppresses replication and neuroinvasion of Venezuelan Equine Encephalitis virus within olfactory sensory neurons. bioRxiv. doi: 10.1101/2023.06.30.547169

PubMed Abstract | Crossref Full Text | Google Scholar

Cain, M. D., Salimi, H., Gong, Y., Yang, L., Hamilton, S. L., Heffernan, J. R., et al. (2017). Virus entry and replication in the brain precedes blood-brain barrier disruption during intranasal alphavirus infection. J. Neuroimmunol. 308, 118–130. doi: 10.1016/j.jneuroim.2017.04.008

PubMed Abstract | Crossref Full Text | Google Scholar

Calisher, C. H. (1994). Medically important arboviruses of the United States and Canada. Clin. Microbiol. Rev. 7, 89–116. doi: 10.1128/CMR.7.1.89

PubMed Abstract | Crossref Full Text | Google Scholar

Calsbeek, J. J., Gonzalez, E. A., Bruun, D. A., Guignet, M. A., Copping, N., Dawson, M. E., et al. (2021). Persistent neuropathology and behavioral deficits in a mouse model of status epilepticus induced by acute intoxication with diisopropylfluorophosphate. Neurotoxicology 87, 106–119. doi: 10.1016/j.neuro.2021.09.001

PubMed Abstract | Crossref Full Text | Google Scholar

Campos, A. S., Franco, A. C., Godinho, F., Huff, R., da Cruz Cardoso, J., Morais, P., et al. (2024). Molecular epidemiology of Western equine encephalitis virus in Brazil, 2023-2024. medRxiv. doi: 10.1101/2024.04.15.24305848

PubMed Abstract | Crossref Full Text | Google Scholar

Cao, X., Yang, D., Parvathareddy, J., Chu, Y. K., Kim, E. J., Fitz-Henley, J. N., et al. (2023). Efficacy of a brain-penetrant antiviral in lethal Venezuelan and eastern equine encephalitis mouse models. Sci. Transl. Med. 15:eabl9344. doi: 10.1126/scitranslmed.abl9344

PubMed Abstract | Crossref Full Text | Google Scholar

Carbonell, W. S., Maris, D. O., McCall, T., and Grady, M. S. (1998). Adaptation of the fluid percussion injury model to the mouse. J. Neurotrauma 15, 217–229. doi: 10.1089/neu.1998.15.217

PubMed Abstract | Crossref Full Text | Google Scholar

Carey, B. D., Akhrymuk, I., Dahal, B., Pinkham, C. L., Bracci, N., Finstuen-Magro, S., et al. (2020). Protein Kinase C subtype delta interacts with Venezuelan equine encephalitis virus capsid protein and regulates viral RNA binding through modulation of capsid phosphorylation. PLoS Pathog. 16:e1008282. doi: 10.1371/journal.ppat.1008282

PubMed Abstract | Crossref Full Text | Google Scholar

Carey, B. D., Ammosova, T., Pinkham, C., Lin, X., Zhou, W., Liotta, L. A., et al. (2018). Protein phosphatase 1alpha interacts with venezuelan equine encephalitis virus capsid protein and regulates viral replication through modulation of capsid phosphorylation. J. Virol. 92. doi: 10.1128/JVI.02068-17

PubMed Abstract | Crossref Full Text | Google Scholar

Carpentier, P., Delamanche, I. S., Le Bert, M., Blanchet, G., and Bouchaud, C. (1990). Seizure-related opening of the blood-brain barrier induced by soman: possible correlation with the acute neuropathology observed in poisoned rats. Neurotoxicology 11, 493–508.

PubMed Abstract | Google Scholar

Carrera, I., Etcheverria, I., Li, Y., Fernandez-Novoa, L., Lombardi, V., Vigo, C., et al. (2013). Immunocytochemical characterization of Alzheimer's disease hallmarks in APP/PS1 transgenic mice treated with a new anti-amyloid-beta vaccine. Cent. Asian J. Glob. Health 2(Suppl):119. doi: 10.5195/cajgh.2013.119

PubMed Abstract | Crossref Full Text | Google Scholar

Carrera, J. P., Bagamian, K. H., Travassos da Rosa, A. P., Wang, E., Beltran, D., Gundaker, N. D., et al. (2018). Human and equine infection with alphaviruses and flaviviruses in panama during 2010: a cross-sectional study of household contacts during an encephalitis outbreak. Am. J. Trop. Med. Hyg. 98, 1798–1804. doi: 10.4269/ajtmh.17-0679

PubMed Abstract | Crossref Full Text | Google Scholar

Carrera, J. P., Forrester, N., Wang, E., Vittor, A. Y., Haddow, A. D., Lopez-Verges, S., et al. (2013). Eastern equine encephalitis in Latin America. N. Engl. J. Med. 369, 732–744. doi: 10.1056/NEJMoa1212628

PubMed Abstract | Crossref Full Text | Google Scholar

Casals, J., Curnen, E. C., and Thomas, L. (1943). Venezuelan Equine Encephalomyelitis in Man. J. Exp. Med. 77, 521–530. doi: 10.1084/jem.77.6.521

PubMed Abstract | Crossref Full Text | Google Scholar

Cassidy, J. D., Carroll, L. J., Peloso, P. M., Borg, J., von Holst, H., Holm, L., et al. (2004). Incidence, risk factors and prevention of mild traumatic brain injury: results of the WHO Collaborating Centre Task Force on Mild Traumatic Brain Injury. J. Rehabil. Med. 28–60. doi: 10.1080/16501960410023732

PubMed Abstract | Crossref Full Text | Google Scholar

CDC (2017). Medical Management Guidelines for Nerve Agents. Tabun (GA); Sarin (GB); Soman (GD); and VX CDC2017. Available at: https://wwwn.cdc.gov/TSP/MMG/MMGDetails.aspx?mmgid=523andtoxid=93 (accessed October 20, 2024).

Google Scholar

CDC (2018). Nerve Agent and Organophosphate Pesticide Poisoning. Atlanta, GA: CDC.

Google Scholar

CDC (2023a). Get the Facts About TBI: Centers for Disease Control and Prevention. Available at: https://www.cdc.gov/traumaticbraininjury/get_the_facts.html (accessed October 30, 2024).

Google Scholar

CDC (2023b). Nerve Agent and Organophosphate Pesticide Poisoning 2023. Available at: https://emergency.cdc.gov/agent/nerve/tsd.asp (accessed October 30, 2024).

Google Scholar

CDC (2024). Traumatic Brain Injury and Concussion. Retrieved from: https://www.cdc.gov/traumatic-brain-injury/ (accessed October 24, 2024).

Google Scholar

Cernak, I. (2005). Animal models of head trauma. NeuroRx 2, 410–422. doi: 10.1602/neurorx.2.3.410

PubMed Abstract | Crossref Full Text | Google Scholar

Cernak, I., Merkle, A. C., Koliatsos, V. E., Bilik, J. M., Luong, Q. T., Mahota, T. M., et al. (2011). The pathobiology of blast injuries and blast-induced neurotrauma as identified using a new experimental model of injury in mice. Neurobiol. Dis. 41, 538–551. doi: 10.1016/j.nbd.2010.10.025

PubMed Abstract | Crossref Full Text | Google Scholar

Cernak, I., Wing, I. D., Davidsson, J., and Plantman, S. (2014). A novel mouse model of penetrating brain injury. Front. Neurol. 5:209. doi: 10.3389/fneur.2014.00209

PubMed Abstract | Crossref Full Text | Google Scholar

Chai, P. R., Boyer, E. W., Al-Nahhas, H., and Erickson, T. B. (2017). Toxic chemical weapons of assassination and warfare: nerve agents VX and sarin. Toxicol. Commun. 1, 21–23. doi: 10.1080/24734306.2017.1373503

PubMed Abstract | Crossref Full Text | Google Scholar

Chang, B. S., Lowenstein, D. H., and Quality standards subcommittee of the american academy of Neurology (2003). Practice parameter: antiepileptic drug prophylaxis in severe traumatic brain injury: report of the Quality Standards Subcommittee of the American Academy of Neurology. Neurology 60, 10–16. doi: 10.1212/01.WNL.0000031432.05543.14

PubMed Abstract | Crossref Full Text | Google Scholar

Chapman, S., Kadar, T., and Gilat, E. (2006). Seizure duration following sarin exposure affects neuro-inflammatory markers in the rat brain. Neurotoxicology 27, 277–283. doi: 10.1016/j.neuro.2005.11.009

PubMed Abstract | Crossref Full Text | Google Scholar

Charles, P. C., Brown, K. W., Davis, N. L., Hart, M. K., and Johnston, R. E. (1997). Mucosal immunity induced by parenteral immunization with a live attenuated Venezuelan equine encephalitis virus vaccine candidate. Virology 228, 153–160. doi: 10.1006/viro.1996.8381

PubMed Abstract | Crossref Full Text | Google Scholar

Chattu, V. K., Manzar, M. D., Kumary, S., Burman, D., Spence, D. W., Pandi-Perumal, S. R., et al. (2018). The global problem of insufficient sleep and its serious public health implications. Healthcare 7:1. doi: 10.3390/healthcare7010001

PubMed Abstract | Crossref Full Text | Google Scholar

Cherra S. J. 3rd, and Chu, C. T.. (2008). Autophagy in neuroprotection and neurodegeneration: a question of balance. Future Neurol. 3, 309–323. doi: 10.2217/14796708.3.3.309

PubMed Abstract | Crossref Full Text | Google Scholar

Chitimus, D. M., Popescu, M. R., Voiculescu, S. E., Panaitescu, A. M., Pavel, B., Zagrean, L., et al. (2020). Melatonin's impact on antioxidative and anti-inflammatory reprogramming in homeostasis and disease. Biomolecules 10:1211. doi: 10.3390/biom10091211

PubMed Abstract | Crossref Full Text | Google Scholar

Choi, Y. J., Kim, J. Y., Yoo, S. B., Lee, J. H., and Jahng, J. W. (2013). Repeated oral administration of capsaicin increases anxiety-like behaviours with prolonged stress-response in rats. J. Biosci. 38, 561–571. doi: 10.1007/s12038-013-9340-9

PubMed Abstract | Crossref Full Text | Google Scholar

Chung, D. H., Jonsson, C. B., Tower, N. A., Chu, Y. K., Sahin, E., Golden, J. E., et al. (2014). Discovery of a novel compound with anti-venezuelan equine encephalitis virus activity that targets the nonstructural protein 2. PLoS Pathog. 10:e1004213. doi: 10.1371/journal.ppat.1004213

PubMed Abstract | Crossref Full Text | Google Scholar

Cieri, M. B., and Ramos, A. J. (2025). Astrocytes, reactive astrogliosis, and glial scar formation in traumatic brain injury. Neural Regen Res. 20, 973–89. doi: 10.4103/NRR.NRR-D-23-02091

PubMed Abstract | Crossref Full Text | Google Scholar

Ciota, A. T. (2022). Eastern equine encephalitis virus taxonomy, genomics, and evolution. J. Med. Entomol. 59, 14–19. doi: 10.1093/jme/tjab079

PubMed Abstract | Crossref Full Text | Google Scholar

Clement J. G. Hand B. T. and Shiloff, J. D. (1981). Differences in the toxicity of soman in various strains of mice. Toxicol. Sci. 1, 419–420. doi: 10.1093/toxsci/1.6.419

Crossref Full Text | Google Scholar

Collins, M. W., Lovell, M. R., Iverson, G. L., Cantu, R. C., Maroon, J. C., Field, M., et al. (2002). Cumulative effects of concussion in high school athletes. Neurosurgery 51, 1175–1179. discussion 80–81. doi: 10.1097/00006123-200211000-00011

PubMed Abstract | Crossref Full Text | Google Scholar

Collombet, J. M., Four, E., Bernabe, D., Masqueliez, C., Burckhart, M. F., Baille, V., et al. (2005). Soman poisoning increases neural progenitor proliferation and induces long-term glial activation in mouse brain. Toxicology 208, 319–334. doi: 10.1016/j.tox.2004.11.036

PubMed Abstract | Crossref Full Text | Google Scholar

Conti, A. C., Raghupathi, R., Trojanowski, J. Q., and McIntosh, T. K. (1998). Experimental brain injury induces regionally distinct apoptosis during the acute and delayed post-traumatic period. J. Neurosci. 18, 5663–5672. doi: 10.1523/JNEUROSCI.18-15-05663.1998

PubMed Abstract | Crossref Full Text | Google Scholar

Costanzi, S., Machado, J. H., and Mitchell, M. (2018). Nerve agents: what they are, how they work, how to counter them. ACS Chem. Neurosci. 9, 873–885. doi: 10.1021/acschemneuro.8b00148

PubMed Abstract | Crossref Full Text | Google Scholar

Covassin, T., Moran, R., and Elbin, R. J. (2016). Sex differences in reported concussion injury rates and time loss from participation: an update of the national collegiate athletic association injury surveillance program from 2004-2005 through 2008-2009. J. Athl. Train. 51, 189–194. doi: 10.4085/1062-6050-51.3.05

PubMed Abstract | Crossref Full Text | Google Scholar

Crosby, B., and Crespo, M. E. (2024). Venezuelan Equine Encephalitis. Treasure Island, FL: StatPearls.

Google Scholar

Cwiklinska, H., Cichalewska-Studzinska, M., Selmaj, K. W., and Mycko, M. P. (2020). The heat shock protein HSP70 promotes Th17 genes' expression via specific regulation of microrna. Int. J. Mol. Sci. 21. doi: 10.3390/ijms21082823

PubMed Abstract | Crossref Full Text | Google Scholar

Dalgard, C. L., Cole, J. T., Kean, W. S., Lucky, J. J., Sukumar, G., McMullen, D. C., et al. (2012). The cytokine temporal profile in rat cortex after controlled cortical impact. Front. Mol. Neurosci. 5:6. doi: 10.3389/fnmol.2012.00006

PubMed Abstract | Crossref Full Text | Google Scholar

Dams-O'Connor, K., Guetta, G., Hahn-Ketter, A. E., and Fedor, A. (2016). Traumatic brain injury as a risk factor for Alzheimer's disease: current knowledge and future directions. Neurodegener. Dis. Manag. 6, 417–429. doi: 10.2217/nmt-2016-0017

PubMed Abstract | Crossref Full Text | Google Scholar

Danes, L., Rychterova, V., Kliment, V., and Hruskova, J. (1973). Penetration of Venezuelan equine encephalomyelitis virus into the brain of guinea pigs and rabbits after intranasal infection. Acta Virol. 17, 138–146.

PubMed Abstract | Google Scholar

Das, M., Mayilsamy, K., Tang, X., Han, J. Y., Foran, E., Willing, A. E., et al. (2019). Pioglitazone treatment prior to transplantation improves the efficacy of human mesenchymal stem cells after traumatic brain injury in rats. Sci. Rep. 9:13646. doi: 10.1038/s41598-019-49428-y

PubMed Abstract | Crossref Full Text | Google Scholar

Davis, A. E. (2000). Mechanisms of traumatic brain injury: biomechanical, structural and cellular considerations. Crit. Care Nurs. Q. 23, 1–13. doi: 10.1097/00002727-200011000-00002

PubMed Abstract | Crossref Full Text | Google Scholar

Davis, N. L., Grieder, F. B., Smith, J. F., Greenwald, G. F., Valenski, M. L., Sellon, D. C., et al. (1994). A molecular genetic approach to the study of Venezuelan equine encephalitis virus pathogenesis. Arch. Virol. Suppl. 9, 99–109. doi: 10.1007/978-3-7091-9326-6_11

PubMed Abstract | Crossref Full Text | Google Scholar

de Araujo Furtado, M., Lumley, L. A., Robison, C., Tong, L. C., Lichtenstein, S., Yourick, D. L., et al. (2010). Spontaneous recurrent seizures after status epilepticus induced by soman in Sprague-Dawley rats. Epilepsia 51, 1503–1510. doi: 10.1111/j.1528-1167.2009.02478.x

PubMed Abstract | Crossref Full Text | Google Scholar

de Araujo Furtado, M., Rossetti, F., Chanda, S., and Yourick, D. (2012). Exposure to nerve agents: from status epilepticus to neuroinflammation, brain damage, neurogenesis and epilepsy. Neurotoxicology 33, 1476–1490. doi: 10.1016/j.neuro.2012.09.001

PubMed Abstract | Crossref Full Text | Google Scholar

de la Monte, S., Castro, F., Bonilla, N. J., Gaskin de Urdaneta, A., and Hutchins, G. M. (1985). The systemic pathology of Venezuelan equine encephalitis virus infection in humans. Am. J. Trop. Med. Hyg. 34, 194–202. doi: 10.4269/ajtmh.1985.34.194

PubMed Abstract | Crossref Full Text | Google Scholar

Deaton, A. V., Craft, S., and Skenazy, J. (1986). Enduring psychiatric and neuropsychologic sequelae in the post-encephalitis patient. Int. J. Psychiatry Med. 16, 275–280. doi: 10.2190/RV2Q-UKFW-UY9L-5D8H

PubMed Abstract | Crossref Full Text | Google Scholar

DeBono, A., Thomas, D. R., Lundberg, L., Pinkham, C., Cao, Y., Graham, J. D., et al. (2019). Novel RU486 (mifepristone) analogues with increased activity against Venezuelan Equine Encephalitis Virus but reduced progesterone receptor antagonistic activity. Sci. Rep. 9:2634. doi: 10.1038/s41598-019-38671-y

PubMed Abstract | Crossref Full Text | Google Scholar

DeFord, S. M., Wilson, M. S., Rice, A. C., Clausen, T., Rice, L. K., Barabnova, A., et al. (2002). Repeated mild brain injuries result in cognitive impairment in B6C3F1 mice. J. Neurotrauma 19, 427–438. doi: 10.1089/08977150252932389

PubMed Abstract | Crossref Full Text | Google Scholar

Delfraro, A., Burgueno, A., Morel, N., Gonzalez, G., Garcia, A., Morelli, J., et al. (2011). Fatal human case of Western equine encephalitis, Uruguay. Emerg. Infect. Dis. 17, 952–954. doi: 10.3201/eid1705.101068

PubMed Abstract | Crossref Full Text | Google Scholar

Delic, V., Beck, K. D., Pang, K. C. H., and Citron, B. A. (2020). Biological links between traumatic brain injury and Parkinson's disease. Acta Neuropathol. Commun. 8:45. doi: 10.1186/s40478-020-00924-7

PubMed Abstract | Crossref Full Text | Google Scholar

Demlie, T. A., Alemu, M. T., Messelu, M. A., Wagnew, F., and Mekonen, E. G. (2023). Incidence and predictors of mortality among traumatic brain injury patients admitted to Amhara region Comprehensive Specialized Hospitals, northwest Ethiopia, 2022. BMC Emerg. Med. 23:55. doi: 10.1186/s12873-023-00823-9

PubMed Abstract | Crossref Full Text | Google Scholar

Despain, K. E., McDonough, J. H., McMonagle, J. D., McGinley, M. J., and Evans, J. (2007). The toxicity of soman in the african green monkey (Chlorocebus aethiops). Toxicol. Mech. Methods 17, 255–264. doi: 10.1080/15376510600972733

PubMed Abstract | Crossref Full Text | Google Scholar

Dewan, M. C., Rattani, A., Gupta, S., Baticulon, R. E., Hung, Y. C., Punchak, M., et al. (2018). Estimating the global incidence of traumatic brain injury. J. Neurosurg. 130, 1080–1097. doi: 10.3171/2017.10.JNS17352

PubMed Abstract | Crossref Full Text | Google Scholar

Dhote, F., Carpentier, P., Barbier, L., Peinnequin, A., Baille, V., Pernot, F., et al. (2012). Combinations of ketamine and atropine are neuroprotective and reduce neuroinflammation after a toxic status epilepticus in mice. Toxicol. Appl. Pharmacol. 259, 195–209. doi: 10.1016/j.taap.2011.12.024

PubMed Abstract | Crossref Full Text | Google Scholar

Dixon, C. E., Kochanek, P. M., Yan, H. Q., Schiding, J. K., Griffith, R. G., Baum, E., et al. (1999). One-year study of spatial memory performance, brain morphology, and cholinergic markers after moderate controlled cortical impact in rats. J. Neurotrauma 16, 109–122. doi: 10.1089/neu.1999.16.109

PubMed Abstract | Crossref Full Text | Google Scholar

Donat, C. K., Scott, G., Gentleman, S. M., and Sastre, M. (2017). Microglial activation in traumatic brain injury. Front. Aging Neurosci. 9:208. doi: 10.3389/fnagi.2017.00208

PubMed Abstract | Crossref Full Text | Google Scholar

Dremov, D. P., and Solianik, R. G. (1977). Use of rabbits for study of the neurovirulence of attenuated variants of eastern equine encephalomyelitis virus. Virologie 28, 263–269.

PubMed Abstract | Google Scholar

Drescher, B., and Bai, F. (2013). Neutrophil in viral infections, friend or foe? Virus Res. 171, 1–7. doi: 10.1016/j.virusres.2012.11.002

PubMed Abstract | Crossref Full Text | Google Scholar

Drevets, W. C., and Rubin, E. H. (1989). Psychotic symptoms and the longitudinal course of senile dementia of the Alzheimer type. Biol. Psychiatry 25, 39–48. doi: 10.1016/0006-3223(89)90145-5

PubMed Abstract | Crossref Full Text | Google Scholar

Duffy, F. H., Burchfiel, J. L., Bartels, P. H., Gaon, M., and Sim, V. M. (1979). Long-term effects of an organophosphate upon the human electroencephalogram. Toxicol. Appl. Pharmacol. 47, 161–176. doi: 10.1016/0041-008X(79)90083-8

PubMed Abstract | Crossref Full Text | Google Scholar

Dulawa, S. C., and Janowsky, D. S. (2019). Cholinergic regulation of mood: from basic and clinical studies to emerging therapeutics. Mol. Psychiatry 24, 694–709. doi: 10.1038/s41380-018-0219-x

PubMed Abstract | Crossref Full Text | Google Scholar

Dupuy, L. C., and Reed, D. S. (2012). Nonhuman primate models of encephalitic alphavirus infection: historical review and future perspectives. Curr. Opin. Virol. 2, 363–367. doi: 10.1016/j.coviro.2012.02.014

PubMed Abstract | Crossref Full Text | Google Scholar

Earnest, M. P., Goolishian, H. A., Calverley, J. R., Hayes, R. O., and Hill, H. R. (1971). Neurologic, intellectual, and psychologic sequelae following western encephalitis. A follow-up study of 35 cases. Neurology 21, 969–974. doi: 10.1212/WNL.21.9.969

PubMed Abstract | Crossref Full Text | Google Scholar

Eassa, N. E., Perez, S. M., Boley, A. M., Elam, H. B., Sharmin, D., Cook, J. M., et al. (2023). alpha5-GABAA receptor modulation reverses behavioral and neurophysiological correlates of psychosis in rats with ventral hippocampal Alzheimer's disease-like pathology. Int. J. Mol. Sci. 24. doi: 10.3390/ijms241411788

PubMed Abstract | Crossref Full Text | Google Scholar

Elder, G. A., and Cristian, A. (2009). Blast-related mild traumatic brain injury: mechanisms of injury and impact on clinical care. Mt. Sinai J. Med. 76, 111–118. doi: 10.1002/msj.20098

PubMed Abstract | Crossref Full Text | Google Scholar

Elhaj, R., and Reynolds, J. M. (2023). Chemical exposures and suspected impact on Gulf War Veterans. Mil. Med. Res. 10:11. doi: 10.1186/s40779-023-00449-9

PubMed Abstract | Crossref Full Text | Google Scholar

Eyolfson, E., Yamakawa, G. R., Griep, Y., Collins, R., Carr, T., Wang, M., et al. (2020). Examining the progressive behavior and neuropathological outcomes associated with chronic repetitive mild traumatic brain injury in rats. Cereb. Cortex. Commun. 1:tgaa002. doi: 10.1093/texcom/tgaa002

PubMed Abstract | Crossref Full Text | Google Scholar

Falchek, S. J. (2012). Encephalitis in the pediatric population. Pediatr. Rev. 33, 122–133. quiz 33. doi: 10.1542/pir.33.3.122

PubMed Abstract | Crossref Full Text | Google Scholar

Fan, S., Loch, A. S., Vongsanga, K., Dennison, G. H., Burn, P. L., Gentle, I. R., et al. (2024). Differentiating between V- and G-series nerve agent and simulant vapours using fluorescent film responses. Small Methods 8:e2301048. doi: 10.1002/smtd.202301048

PubMed Abstract | Crossref Full Text | Google Scholar

Fann, J. R., Burington, B., Leonetti, A., Jaffe, K., Katon, W. J., Thompson, R. S., et al. (2004). Psychiatric illness following traumatic brain injury in an adult health maintenance organization population. Arch. Gen. Psychiatry 61, 53–61. doi: 10.1001/archpsyc.61.1.53

PubMed Abstract | Crossref Full Text | Google Scholar

Farber, S., Hill, A., Connerly, M. L., and Dingle, J. H. (1940). Encephalitis in infants and children: caused by the virus of the eastern variety of equine encephalitis. J. Am. Med. Assoc. 114, 1725–1731. doi: 10.1001/jama.1940.02810180001001

Crossref Full Text | Google Scholar

Faul, M., Xu, L., Wald, M. M., and Coronado, V. G. (2010). Traumatic Brain Injury in the United States. Atlanta, GA: Centers for Disiease Control and Prevention (CDC).

Google Scholar

Fawcett, W. P., Aracava, Y., Adler, M., Pereira, E. F., and Albuquerque, E. X. (2009). Acute toxicity of organophosphorus compounds in guinea pigs is sex- and age-dependent and cannot be solely accounted for by acetylcholinesterase inhibition. J. Pharmacol. Exp. Ther. 328, 516–524. doi: 10.1124/jpet.108.146639

PubMed Abstract | Crossref Full Text | Google Scholar

Fehily, B., and Fitzgerald, M. (2017). Repeated mild traumatic brain injury: potential mechanisms of damage. Cell Transplant. 26, 1131–1155. doi: 10.1177/0963689717714092

PubMed Abstract | Crossref Full Text | Google Scholar

Fesharaki-Zadeh, A. (2022). Oxidative stress in traumatic brain injury. Int. J. Mol. Sci. 23. doi: 10.3390/ijms232113000

PubMed Abstract | Crossref Full Text | Google Scholar

Figueiredo, T. H., Apland, J. P., Braga, M. F. M., and Marini, A. M. (2018). Acute and long-term consequences of exposure to organophosphate nerve agents in humans. Epilepsia 59(Suppl 2), 92–99. doi: 10.1111/epi.14500

PubMed Abstract | Crossref Full Text | Google Scholar

Filliat, P., Baubichon, D., Burckhart, M. F., Pernot-Marino, I., Foquin, A., Masqueliez, C., et al. (1999). Memory impairment after soman intoxication in rat: correlation with central neuropathology. Improvement with anticholinergic and antiglutamatergic therapeutics. Neurotoxicology 20, 535–549.

PubMed Abstract | Google Scholar

Filliat, P., Coubard, S., Pierard, C., Liscia, P., Beracochea, D., Four, E., et al. (2007). Long-term behavioral consequences of soman poisoning in mice. Neurotoxicology 28, 508–519. doi: 10.1016/j.neuro.2006.11.004

PubMed Abstract | Crossref Full Text | Google Scholar

Finnegan, T. J., Gunawardana, V. W. L., and Badjic, J. D. (2021). Molecular recognition of nerve agents and their organophosphorus surrogates: toward supramolecular scavengers and catalysts. Chemistry 27, 13280–13305. doi: 10.1002/chem.202101532

PubMed Abstract | Crossref Full Text | Google Scholar

Fongsaran, C., Jirakanwisal, K., Peng, B. H., Fracassi, A., Taglialatela, G., Dineley, K. T., et al. (2024). Arbovirus infection increases the risk for the development of neurodegenerative disease pathology in the murine model. Brain Behav Immun Health 38:100780. doi: 10.1016/j.bbih.2024.100780

PubMed Abstract | Crossref Full Text | Google Scholar

Food and Drug Administration (2021). Traumatic Brain Injury: What to Know About Symptoms, Diagnosis, and Treatment. Silver Spring, MD: FDA.

Google Scholar

Fordington, S., and Manford, M. A. (2020). review of seizures and epilepsy following traumatic brain injury. J. Neurol. 267, 3105–3111. doi: 10.1007/s00415-020-09926-w

PubMed Abstract | Crossref Full Text | Google Scholar

Fox, G. B., LeVasseur, R. A., and Faden, A. I. (1999). Behavioral responses of C57BL/6, FVB/N, and 129/SvEMS mouse strains to traumatic brain injury: implications for gene targeting approaches to neurotrauma. J. Neurotrauma 16, 377–389. doi: 10.1089/neu.1999.16.377

PubMed Abstract | Crossref Full Text | Google Scholar

Francois, S., Mondot, S., Gerard, Q., Bel, R., Knoertzer, J., Berriche, A., et al. (2022). Long-term anxiety-like behavior and microbiota changes induced in mice by sublethal doses of acute sarin surrogate exposure. Biomedicines 10:1167. doi: 10.3390/biomedicines10051167

PubMed Abstract | Crossref Full Text | Google Scholar

Frey, L. C. (2003). Epidemiology of posttraumatic epilepsy: a critical review. Epilepsia 44, 11–17. doi: 10.1046/j.1528-1157.44.s10.4.x

PubMed Abstract | Crossref Full Text | Google Scholar

Fulton, J. S., and Burton, A. N. (1953). After effects of western equine encephalomyelitis infection in man. Can. Med. Assoc. J. 69, 268–272.

Google Scholar

Furman, R. A., Garrett, L. T., Rapp, M. C., Watson, G. D., and Lucot, B. J. A. (2014). comparison of the sensitivityof different strains of mice to sarin. MMSL 83, 90–96. doi: 10.31482/mmsl.2014.018

PubMed Abstract | Crossref Full Text | Google Scholar

Gage, M., Rao, N. S., Samidurai, M., Putra, M., Vasanthi, S. S., Meyer, C., et al. (2021). Soman (GD) rat model to mimic civilian exposure to nerve agent: mortality, video-eeg based status epilepticus severity, sex differences, spontaneously recurring seizures, and brain pathology. Front. Cell. Neurosci. 15:798247. doi: 10.3389/fncel.2021.798247

PubMed Abstract | Crossref Full Text | Google Scholar

Galgano, M., Toshkezi, G., Qiu, X., Russell, T., Chin, L., Zhao, L. R., et al. (2017). Traumatic brain injury: current treatment strategies and future endeavors. Cell Transplant 26, 1118–1130. doi: 10.1177/0963689717714102

PubMed Abstract | Crossref Full Text | Google Scholar

Gall, B., Pryke, K., Abraham, J., Mizuno, N., Botto, S., Sali, T. M., et al. (2018). Emerging alphaviruses are sensitive to cellular states induced by a novel small-molecule agonist of the STING pathway. J. Virol. 92. doi: 10.1128/JVI.01913-17

PubMed Abstract | Crossref Full Text | Google Scholar

Garcia, Y., and Esquivel, N. (2018). Comparison of the response of male BALB/c and C57BL/6 mice in behavioral tasks to evaluate cognitive function. Behav Sci. 8. doi: 10.3390/bs8010014

PubMed Abstract | Crossref Full Text | Google Scholar

Gardner, C. L., Burke, C. W., Tesfay, M. Z., Glass, P. J., Klimstra, W. B., Ryman, K. D., et al. (2008). Eastern and Venezuelan equine encephalitis viruses differ in their ability to infect dendritic cells and macrophages: impact of altered cell tropism on pathogenesis. J. Virol. 82, 10634–10646. doi: 10.1128/JVI.01323-08

PubMed Abstract | Crossref Full Text | Google Scholar

Gardner, C. L., Sun, C., Dunn, M. D., Gilliland, T. C. Jr., Trobaugh, D. W., Terada, Y., et al. (2022). In vitro and in vivo phenotypes of venezuelan, eastern and western equine encephalitis viruses derived from cDNA clones of human isolates. Viruses 15:5. doi: 10.3390/v15010005

PubMed Abstract | Crossref Full Text | Google Scholar

Gardner, R. C., and Yaffe, K. (2015). Epidemiology of mild traumatic brain injury and neurodegenerative disease. Mol. Cell Neurosci. 66(Pt B), 75–80. doi: 10.1016/j.mcn.2015.03.001

PubMed Abstract | Crossref Full Text | Google Scholar

Garnett, M. R., Blamire, A. M., Corkill, R. G., Cadoux-Hudson, T. A., Rajagopalan, B., Styles, P., et al. (2000a). Early proton magnetic resonance spectroscopy in normal-appearing brain correlates with outcome in patients following traumatic brain injury. Brain 123, 2046–2054. doi: 10.1093/brain/123.10.2046

PubMed Abstract | Crossref Full Text | Google Scholar

Garnett, M. R., Blamire, A. M., Rajagopalan, B., Styles, P., and Cadoux-Hudson, T. A. (2000b). Evidence for cellular damage in normal-appearing white matter correlates with injury severity in patients following traumatic brain injury: a magnetic resonance spectroscopy study. Brain 123, 1403–1409. doi: 10.1093/brain/123.7.1403

PubMed Abstract | Crossref Full Text | Google Scholar

Gennarelli, T. A., Thibault, L. E., Tomei, G., Wiser, R., Graham, D., Adams, J., et al. (1987). Directional dependence of axonal brain injury due to centroidal and non-centroidal acceleration. SAE Transactions. 96, 1355–1359. doi: 10.4271/872197

Crossref Full Text | Google Scholar

Genovese, R. F., Oubre, J. L., Jakubowski, E. M., Fleming, P. J., Saxena, A., Rockwood, G. A., et al. (2007). Evaluation of cognitive and biochemical effects of low-level exposure to sarin in rhesus and African green monkeys. Toxicology 231, 11–20. doi: 10.1016/j.tox.2006.10.018

PubMed Abstract | Crossref Full Text | Google Scholar

Gleiser, C. A., Gochenour, W. S. Jr., Berge, T. O., and Tigertt, W. D. (1961). Studies on the virus of Venezuelan equine encephalomyelitis. I. Modification by cortisone of the response of the central nervous system of Macaca mulatta. J. Immunol. 87, 504–508. doi: 10.4049/jimmunol.87.5.504

PubMed Abstract | Crossref Full Text | Google Scholar

Glushakova, O. Y., Johnson, D., and Hayes, R. L. (2014). Delayed increases in microvascular pathology after experimental traumatic brain injury are associated with prolonged inflammation, blood-brain barrier disruption, and progressive white matter damage. J. Neurotrauma 31, 1180–1193. doi: 10.1089/neu.2013.3080

PubMed Abstract | Crossref Full Text | Google Scholar

Gold, E. M., Vasilevko, V., Hasselmann, J., Tiefenthaler, C., Hoa, D., Ranawaka, K., et al. (2018). Repeated mild closed head injuries induce long-term white matter pathology and neuronal loss that are correlated with behavioral deficits. ASN Neuro. 10:1759091418781921. doi: 10.1177/1759091418781921

PubMed Abstract | Crossref Full Text | Google Scholar

Goldman, M. W., Kawakami, B. W., Rosenblatt, T. G., Culbertson, L. S., Schreider, M. R., Remsen, J. P., et al. (1988). Toxicity studies on agent VX. Final report (AD A201397) Prepared by the Laboratory for Energy-Related Health Research. The National Academies Press.

Google Scholar

Golime, R., Palit, M., Acharya, J., and Dubey, D. K. (2018). Neuroprotective effects of galantamine on nerve agent-induced neuroglial and biochemical changes. Neurotox. Res. 33, 738–748. doi: 10.1007/s12640-017-9815-9

PubMed Abstract | Crossref Full Text | Google Scholar

Gordon, J. J., Leadbeater, L., and Maidment, M. P. (1978). The protection of animals against organophosphate poisoning by pretreatment with a carbamate. Toxicol. Appl. Pharmacol. 43, 207–216. doi: 10.1016/S0041-008X(78)80045-3

PubMed Abstract | Crossref Full Text | Google Scholar

Gouvea Bogossian, E., Peluso, L., Creteur, J., and Taccone, F. S. (2020). Hyperventilation in adult TBI patients: how to approach it? Front. Neurol. 11:580859. doi: 10.3389/fneur.2020.580859

PubMed Abstract | Crossref Full Text | Google Scholar

Green, R. E. A., Dabek, M. K., Changoor, A., Rybkina, J., Monette, G. A., Colella, B., et al. (2023). Moderate-severe TBI as a progressive disorder: patterns and predictors of cognitive declines in the chronic stages of injury. Neurorehabil. Neural. Repair. 37, 799–809. doi: 10.1177/15459683231212861

PubMed Abstract | Crossref Full Text | Google Scholar

Green, T. R. F., Carey, S. D., Mannino, G., Craig, J. A., Rowe, R. K., Zielinski, M. R., et al. (2024). Sleep, inflammation, and hemodynamics in rodent models of traumatic brain injury. Front. Neurosci. 18:1361014. doi: 10.3389/fnins.2024.1361014

PubMed Abstract | Crossref Full Text | Google Scholar

Grieder, F. B., Davis, B. K., Zhou, X. D., Chen, S. J., Finkelman, F. D., Gause, W. C., et al. (1997). Kinetics of cytokine expression and regulation of host protection following infection with molecularly cloned Venezuelan equine encephalitis virus. Virology 233, 302–312. doi: 10.1006/viro.1997.8617

PubMed Abstract | Crossref Full Text | Google Scholar

Gunnell, D., Eddleston, M., Phillips, M. R., and Konradsen, F. (2007). The global distribution of fatal pesticide self-poisoning: systematic review. BMC Public Health 7:357. doi: 10.1186/1471-2458-7-357

PubMed Abstract | Crossref Full Text | Google Scholar

Gupta, R. C. (2020). “Neurotoxicity of organophosphate nerve agents,” in Advances in Neurotoxicology, 4th Edn, eds. M. Aschner, and L. G. Costa (Cambridge, MA: Academic Press), 79–112. doi: 10.1016/bs.ant.2019.11.001

Crossref Full Text | Google Scholar

Gupte, R., Brooks, W., Vukas, R., Pierce, J., and Harris, J. (2019). Sex differences in traumatic brain injury: what we know and what we should know. J. Neurotrauma 36, 3063–3091. doi: 10.1089/neu.2018.6171

PubMed Abstract | Crossref Full Text | Google Scholar

Guskiewicz, K. M., Marshall, S. W., Bailes, J., McCrea, M., Cantu, R. C., Randolph, C., et al. (2005). Association between recurrent concussion and late-life cognitive impairment in retired professional football players. Neurosurgery 57, 719–724. doi: 10.1227/01.NEU.0000175725.75780.DD

PubMed Abstract | Crossref Full Text | Google Scholar

Guskiewicz, K. M., McCrea, M., Marshall, S. W., Cantu, R. C., Randolph, C., Barr, W., et al. (2003). Cumulative effects associated with recurrent concussion in collegiate football players: the NCAA Concussion Study. JAMA 290, 2549–2555. doi: 10.1001/jama.290.19.2549

PubMed Abstract | Crossref Full Text | Google Scholar

Guzmán-Terán, C., Calderón-Rangel, A., Rodriguez-Morales, A., and Mattar, S. (2020). Venezuelan equine encephalitis virus: the problem is not over for tropical America. Ann. Clin. Microbiol. Antimicrob. 19:19. doi: 10.1186/s12941-020-00360-4

PubMed Abstract | Crossref Full Text | Google Scholar

Hahn, Y. S., Fuchs, S., Flannery, A. M., Barthel, M. J., and McLone, D. G. (1988). Factors influencing posttraumatic seizures in children. Neurosurgery 22, 864–867. doi: 10.1227/00006123-198805000-00008

Crossref Full Text | Google Scholar

Haley, R. W., Dever, J. A., and Teiber, J. F. (2022). Response to “Comment on ‘evaluation of a gene-environment interaction of PON1 and low-level nerve agent exposure with Gulf War illness: a prevalence case-control study drawn from the U.S. Military Health Survey's National Population Sample”'. Environ. Health Perspect. 130:68004. doi: 10.1289/EHP11607

PubMed Abstract | Crossref Full Text | Google Scholar

Hanson, R. P., Sulkin, S. E., Beuscher, E. L., Hammon, W. M., McKinney, R. W., Work, T. H., et al. (1967). Arbovirus infections of laboratory workers. Extent of problem emphasizes the need for more effective measures to reduce hazards. Science 158, 1283–1286. doi: 10.1126/science.158.3806.1283

PubMed Abstract | Crossref Full Text | Google Scholar

Hart, M. K., Caswell-Stephan, K., Bakken, R., Tammariello, R., Pratt, W., Davis, N., et al. (2000). Improved mucosal protection against Venezuelan equine encephalitis virus is induced by the molecularly defined, live-attenuated V3526 vaccine candidate. Vaccine 18, 3067–3075. doi: 10.1016/S0264-410X(00)00042-6

PubMed Abstract | Crossref Full Text | Google Scholar

Hart, M. K., Pratt, W., Panelo, F., Tammariello, R., and Dertzbaugh, M. (1997). Venezuelan equine encephalitis virus vaccines induce mucosal IgA responses and protection from airborne infection in BALB/c, but not C3H/HeN mice. Vaccine 15, 363–369. doi: 10.1016/S0264-410X(96)00204-6

PubMed Abstract | Crossref Full Text | Google Scholar

Haslam, J. D., Russell, P., Hill, S., Emmett, S. R., and Blain, P. G. (2022). Chemical, biological, radiological, and nuclear mass casualty medicine: a review of lessons from the Salisbury and Amesbury Novichok nerve agent incidents. Br. J. Anaesth. 128, e200–e205. doi: 10.1016/j.bja.2021.10.008

PubMed Abstract | Crossref Full Text | Google Scholar

Hayoun, M. A., Smith, M. E., Ausman, C., Yarrarapu, S. N. S., and Swoboda, H. D. (2024). Toxicology, V-Series Nerve Agents. Treasure Island, FL: StatPearls.

Google Scholar

Hellal, F., Bonnefont-Rousselot, D., Croci, N., Palmier, B., Plotkine, M., Marchand-Verrecchia, C., et al. (2004). Pattern of cerebral edema and hemorrhage in a mice model of diffuse brain injury. Neurosci. Lett. 357, 21–24. doi: 10.1016/j.neulet.2003.12.036

PubMed Abstract | Crossref Full Text | Google Scholar

Henderson, R. F., Barr, E. B., Blackwell, W. B., Clark, C. R., Conn, C. A., Kalra, R., et al. (2002). Response of rats to low levels of sarin. Toxicol. Appl. Pharmacol. 184, 67–76. doi: 10.1006/taap.2002.9495

Crossref Full Text | Google Scholar

Herzon, H., Shelton, J. T., and Bruyn, H. B. (1957). Sequelae of western equine and other arthropod-borne encephalitides. Neurology 7, 535–548. doi: 10.1212/WNL.7.8.535

PubMed Abstract | Crossref Full Text | Google Scholar

Hiebert, J. B., Shen, Q., Thimmesch, A. R., and Pierce, J. D. (2015). Traumatic brain injury and mitochondrial dysfunction. Am. J. Med. Sci. 350, 132–138. doi: 10.1097/MAJ.0000000000000506

PubMed Abstract | Crossref Full Text | Google Scholar

Hill, V., Koch, R. T., Bialosuknia, S. M., Ngo, K., Zink, S. D., Koetzner, C. A., et al. (2023). Dynamics of Eastern equine encephalitis virus during the 2019 outbreak in the Northeast United States. medRxiv. doi: 10.1016/j.cub.2023.05.047

PubMed Abstract | Crossref Full Text | Google Scholar

Ho, K. M., Honeybul, S., Yip, C. B., and Silbert, B. I. (2014). Prognostic significance of blood-brain barrier disruption in patients with severe nonpenetrating traumatic brain injury requiring decompressive craniectomy. J. Neurosurg. 121, 674–679. doi: 10.3171/2014.6.JNS132838

PubMed Abstract | Crossref Full Text | Google Scholar

Hoard-Fruchey, H. M., Rothwell, C., Dillman, J. F., and Kan, R. K. (2020). Chemical Warfare Nerve Agent-Induced Effects on Gene Expression in Human Neuron and Astrocyte Cultures Defense Technical Information Center.

Google Scholar

Hollidge, B. S., Cohen, C. A., Akuoku Frimpong, J., Badger, C. V., Dye, J. M., Schmaljohn, C. S., et al. (2021). Toll-like receptor 4 mediates blood-brain barrier permeability and disease in C3H mice during Venezuelan equine encephalitis virus infection. Virulence 12, 430–443. doi: 10.1080/21505594.2020.1870834

PubMed Abstract | Crossref Full Text | Google Scholar

Honnold, S. P., Mossel, E. C., Bakken, R. R., Fisher, D., Lind, C. M., Cohen, J. W., et al. (2015a). Eastern equine encephalitis virus in mice I: clinical course and outcome are dependent on route of exposure. Virol. J. 12:152. doi: 10.1186/s12985-015-0386-1

PubMed Abstract | Crossref Full Text | Google Scholar

Honnold, S. P., Mossel, E. C., Bakken, R. R., Lind, C. M., Cohen, J. W., Eccleston, L. T., et al. (2015b). Eastern equine encephalitis virus in mice II: pathogenesis is dependent on route of exposure. Virol. J. 12:154. doi: 10.1186/s12985-015-0385-2

PubMed Abstract | Crossref Full Text | Google Scholar

Howlett, J. R., Nelson, L. D., and Stein, M. B. (2022). Mental health consequences of traumatic brain injury. Biol. Psychiatry. 91, 413–420. doi: 10.1016/j.biopsych.2021.09.024

PubMed Abstract | Crossref Full Text | Google Scholar

Hrvat, N. M., and Kovarik, Z. (2020). Counteracting poisoning with chemical warfare nerve agents. Arh. Hig. Rada Toksikol. 71, 266–284. doi: 10.2478/aiht-2020-71-3459

PubMed Abstract | Crossref Full Text | Google Scholar

Hubbard, W. B., Joseph, B., Spry, M., Vekaria, H. J., Saatman, K. E., Sullivan, P. G., et al. (2019). Acute mitochondrial impairment underlies prolonged cellular dysfunction after repeated mild traumatic brain injuries. J. Neurotrauma 36, 1252–1263. doi: 10.1089/neu.2018.5990

PubMed Abstract | Crossref Full Text | Google Scholar

Husain, A., and Byrareddy, S. N. (2020). Rapamycin as a potential repurpose drug candidate for the treatment of COVID-19. Chem. Biol. Interact. 331:109282. doi: 10.1016/j.cbi.2020.109282

PubMed Abstract | Crossref Full Text | Google Scholar

Iaccarino, C., Carretta, A., Nicolosi, F., and Morselli, C. (2018). Epidemiology of severe traumatic brain injury. J. Neurosurg. Sci. 62, 535–541. doi: 10.23736/S0390-5616.18.04532-0

PubMed Abstract | Crossref Full Text | Google Scholar

Jackson, A. C., SenGupta, S. K., and Smith, J. F. (1991). Pathogenesis of Venezuelan equine encephalitis virus infection in mice and hamsters. Vet. Pathol. 28, 410–418. doi: 10.1177/030098589102800509

PubMed Abstract | Crossref Full Text | Google Scholar

Jain, S., and Iverson, L. M. (2023). Glasgow Coma Scale. Treasure Island, FL: StatPearls.

Google Scholar

Jang, Y. J., Kim, K., Tsay, O. G., Atwood, D. A., and Churchill, D. G. (2015). Update 1 of: destruction and detection of chemical warfare agents. Chem. Rev. 115, R1–R76. doi: 10.1021/acs.chemrev.5b00402

PubMed Abstract | Crossref Full Text | Google Scholar

Jett, D. A., Sibrizzi, C. A., Blain, R. B., Hartman, P. A., Lein, P. J., Taylor, K. W., et al. (2020). A national toxicology program systematic review of the evidence for long-term effects after acute exposure to sarin nerve agent. Crit. Rev. Toxicol. 50, 474–490. doi: 10.1080/10408444.2020.1787330

PubMed Abstract | Crossref Full Text | Google Scholar

John, H., van der Schans, M. J., Koller, M., Spruit, H. E. T., Worek, F., Thiermann, H., et al. (2018). Fatal sarin poisoning in Syria 2013: forensic verification within an international laboratory network. Forensic Toxicol. 36, 61–71. doi: 10.1007/s11419-017-0376-7

PubMed Abstract | Crossref Full Text | Google Scholar

Johnson, E. A., Dao, T. L., Guignet, M. A., Geddes, C. E., Koemeter-Cox, A. I., Kan, R. K., et al. (2011). Increased expression of the chemokines CXCL1 and MIP-1alpha by resident brain cells precedes neutrophil infiltration in the brain following prolonged soman-induced status epilepticus in rats. J. Neuroinflammation. 8:41. doi: 10.1186/1742-2094-8-41

PubMed Abstract | Crossref Full Text | Google Scholar

Johnson, E. A., Guignet, M. A., Dao, T. L., Hamilton, T. A., and Kan, R. K. (2015). Interleukin-18 expression increases in response to neurovascular damage following soman-induced status epilepticus in rats. J Inflamm. 12:43. doi: 10.1186/s12950-015-0089-9

PubMed Abstract | Crossref Full Text | Google Scholar

Johnson, E. A., and Kan, R. K. (2010). The acute phase response and soman-induced status epilepticus: temporal, regional and cellular changes in rat brain cytokine concentrations. J. Neuroinflammation 7:40. doi: 10.1186/1742-2094-7-40

PubMed Abstract | Crossref Full Text | Google Scholar

Jones, N. C., Cardamone, L., Williams, J. P., Salzberg, M. R., Myers, D., O'Brien, T. J., et al. (2008). Experimental traumatic brain injury induces a pervasive hyperanxious phenotype in rats. J. Neurotrauma 25, 1367–1374. doi: 10.1089/neu.2008.0641

PubMed Abstract | Crossref Full Text | Google Scholar

Jonsson, C. B., Cao, X., Lee, J., Gabbard, J. D., Chu, Y. K., Fitzpatrick, E. A., et al. (2019). Efficacy of a ML336 derivative against Venezuelan and eastern equine encephalitis viruses. Antiviral Res. 167, 25–34. doi: 10.1016/j.antiviral.2019.04.004

PubMed Abstract | Crossref Full Text | Google Scholar

Joshi, V. V., Patel, N. D., Rehan, M. A., and Kuppa, A. (2019). Mysterious mechanisms of memory formation: are the answers hidden in synapses? Cureus 11:e5795. doi: 10.7759/cureus.5795

PubMed Abstract | Crossref Full Text | Google Scholar

Julander, J. G., Skirpstunas, R., Siddharthan, V., Shafer, K., Hoopes, J. D., Smee, D. F., et al. (2008). C3H/HeN mouse model for the evaluation of antiviral agents for the treatment of Venezuelan equine encephalitis virus infection. Antiviral Res. 78, 230–241. doi: 10.1016/j.antiviral.2008.01.007

PubMed Abstract | Crossref Full Text | Google Scholar

Kadar, T., Shapira, S., Cohen, G., Sahar, R., Alkalay, D., Raveh, L., et al. (1995). Sarin-induced neuropathology in rats. Hum. Exp. Toxicol. 14, 252–259. doi: 10.1177/096032719501400304

PubMed Abstract | Crossref Full Text | Google Scholar

Kanner, A. M., and Bicchi, M. M. (2022). Antiseizure medications for adults with epilepsy: a review. JAMA. 327, 1269–1281. doi: 10.1001/jama.2022.3880

PubMed Abstract | Crossref Full Text | Google Scholar

Kassa, J., Bajgar, J., Kuca, K., Musilek, K., and Karasova, J. (2008). The present approaches to the development of prophylactic and therapeutic antidotes against nerve agents. Interdiscip. Toxicol. 1, 18–21. doi: 10.2478/v10102-010-0026-y

PubMed Abstract | Crossref Full Text | Google Scholar

Kassa, J., Koupilova, M., and Vachek, J. (2001). The influence of low-level sarin inhalation exposure on spatial memory in rats. Pharmacol. Biochem. Behav. 70, 175–179. doi: 10.1016/S0091-3057(01)00592-5

PubMed Abstract | Crossref Full Text | Google Scholar

Kassa, J., and Vachek, J. A. (2002). comparison of the efficacy of pyridostigmine alone and the combination of pyridostigmine with anticholinergic drugs as pharmacological pretreatment of tabun-poisoned rats and mice. Toxicology 177, 179–185. doi: 10.1016/S0300-483X(02)00219-6

PubMed Abstract | Crossref Full Text | Google Scholar

Keck, F., Brooks-Faulconer, T., Lark, T., Ravishankar, P., Bailey, C., Salvador-Morales, C., et al. (2017). Altered mitochondrial dynamics as a consequence of Venezuelan Equine encephalitis virus infection. Virulence 8, 1849–1866. doi: 10.1080/21505594.2016.1276690

PubMed Abstract | Crossref Full Text | Google Scholar

Keck, F., Kortchak, S., Bakovic, A., Roberts, B., Agrawal, N., Narayanan, A., et al. (2018). Direct and indirect pro-inflammatory cytokine response resulting from TC-83 infection of glial cells. Virulence 9, 1403–1421. doi: 10.1080/21505594.2018.1509668

PubMed Abstract | Crossref Full Text | Google Scholar

Kehn-Hall, K., and Bradfute, S. B. (2022). Understanding host responses to equine encephalitis virus infection: implications for therapeutic development. Expert Rev. Anti Infect. Ther. 20, 1551–1566. doi: 10.1080/14787210.2022.2141224

PubMed Abstract | Crossref Full Text | Google Scholar

Kelsey, N. A., Wilkins, H. M., and Linseman, D. A. (2010). Nutraceutical antioxidants as novel neuroprotective agents. Molecules 15, 7792–7814. doi: 10.3390/molecules15117792

PubMed Abstract | Crossref Full Text | Google Scholar

Kendrick, D. J., Mishra, R. C., John, C. M., Zhu, H. L., and Braun, A. P. (2021). Effects of pharmacological inhibitors of NADPH oxidase on myogenic contractility and evoked vasoactive responses in rat resistance arteries. Front. Physiol. 12:752366. doi: 10.3389/fphys.2021.752366

PubMed Abstract | Crossref Full Text | Google Scholar

Khan, S., Ahmad, K., Alshammari, E. M., Adnan, M., Baig, M. H., Lohani, M., et al. (2015). Implication of caspase-3 as a common therapeutic target for multineurodegenerative disorders and its inhibition using nonpeptidyl natural compounds. Biomed Res. Int. 2015:379817. doi: 10.1155/2015/379817

PubMed Abstract | Crossref Full Text | Google Scholar

Kim, S. Y., Yeh, P. H., Ollinger, J. M., Morris, H. D., Hood, M. N., Ho, V. B., et al. (2023). Military-related mild traumatic brain injury: clinical characteristics, advanced neuroimaging, and molecular mechanisms. Transl. Psychiatry. 13:289. doi: 10.1038/s41398-023-02569-1

PubMed Abstract | Crossref Full Text | Google Scholar

King, C., Robinson, T., Dixon, C. E., Rao, G. R., Larnard, D., Nemoto, C. E., et al. (2010). Brain temperature profiles during epidural cooling with the ChillerPad in a monkey model of traumatic brain injury. J. Neurotrauma 27, 1895–1903. doi: 10.1089/neu.2009.1178

PubMed Abstract | Crossref Full Text | Google Scholar

Klass, D. W., and Westmoreland, B. F. (1985). Nonepileptogenic epileptiform electroencephalographic activity. Ann. Neurol. 18, 627–635. doi: 10.1002/ana.410180602

PubMed Abstract | Crossref Full Text | Google Scholar

Knox, C., Law, V., Jewison, T., Liu, P., Ly, S., Frolkis, A., et al. (2011). DrugBank 3.0: a comprehensive resource for 'omics' research on drugs. Nucleic Acids Res. 39(Database issue), D1035–D1041. doi: 10.1093/nar/gkq1126

PubMed Abstract | Crossref Full Text | Google Scholar

Knox, C., Wilson, M., Klinger, C. M., Franklin, M., Oler, E., Wilson, A., et al. (2024). DrugBank 6.0: the DrugBank knowledgebase for 2024. Nucleic Acids Res. 52, D1265–D1275. doi: 10.1093/nar/gkad976

PubMed Abstract | Crossref Full Text | Google Scholar

Kochanek, P. M., Clark, R. S., Ruppel, R. A., Adelson, P. D., Bell, M. J., Whalen, M. J., et al. (2000). Biochemical, cellular, and molecular mechanisms in the evolution of secondary damage after severe traumatic brain injury in infants and children: lessons learned from the bedside. Pediatr. Crit. Care Med. 1, 4–19. doi: 10.1097/00130478-200007000-00003

PubMed Abstract | Crossref Full Text | Google Scholar

Kochanek, P. M., Jackson, T. C., Jha, R. M., Clark, R. S. B., Okonkwo, D. O., Bayir, H., et al. (2020). Paths to successful translation of new therapies for severe traumatic brain injury in the golden age of traumatic brain injury research: a Pittsburgh vision. J. Neurotrauma 37, 2353–2371. doi: 10.1089/neu.2018.6203

PubMed Abstract | Crossref Full Text | Google Scholar

Konan, L. M., Song, H., Pentecost, G., Fogwe, D., Ndam, T., Cui, J., et al. (2019). Multi-focal neuronal ultrastructural abnormalities and synaptic alterations in mice after low-intensity blast exposure. J. Neurotrauma 36, 2117–2128. doi: 10.1089/neu.2018.6260

PubMed Abstract | Crossref Full Text | Google Scholar

Korczyn, A. D., and Grinberg, L. T. (2024). Is Alzheimer disease a disease? Nat. Rev. Neurol. 20, 245–51. doi: 10.1038/s41582-024-00940-4

PubMed Abstract | Crossref Full Text | Google Scholar

Koterski, J., Twenhafel, N., Porter, A., Reed, D. S., Martino-Catt, S., Sobral, B., et al. (2007). Gene expression profiling of nonhuman primates exposed to aerosolized Venezuelan equine encephalitis virus. FEMS Immunol. Med. Microbiol. 51, 462–472. doi: 10.1111/j.1574-695X.2007.00319.x

PubMed Abstract | Crossref Full Text | Google Scholar

Kumar, A., Stoica, B. A., Sabirzhanov, B., Burns, M. P., Faden, A. I., Loane, D. J., et al. (2013). Traumatic brain injury in aged animals increases lesion size and chronically alters microglial/macrophage classical and alternative activation states. Neurobiol. Aging. 34, 1397–1411. doi: 10.1016/j.neurobiolaging.2012.11.013

PubMed Abstract | Crossref Full Text | Google Scholar

Lalkovicova, M., and Danielisova, V. (2016). Neuroprotection and antioxidants. Neural Regen Res. 11, 865–74. doi: 10.4103/1673-5374.184447

PubMed Abstract | Crossref Full Text | Google Scholar

Lallement, G., Foquin, A., Dorandeu, F., Baubichon, D., Aubriot, S., Carpentier, P., et al. (2001). Subchronic administration of various pretreatments of nerve agent poisoning. I. Protection of blood and central cholinesterases, innocuousness towards blood-brain barrier permeability. Drug Chem. Toxicol. 24, 151–164. doi: 10.1081/DCT-100102607

PubMed Abstract | Crossref Full Text | Google Scholar

Langsjoen, R. M., Key, A., Shariatzadeh, N., Jackson, C. R., Mahmood, F., Arkun, K., et al. (2023). Eastern equine encephalitis virus diversity in massachusetts patients, 1938-2020. Am. J. Trop. Med. Hyg. 109, 387–396. doi: 10.4269/ajtmh.23-0047

PubMed Abstract | Crossref Full Text | Google Scholar

Langston, J. L., and Myers, T. M. (2016). VX toxicity in the Gottingen minipig. Toxicol. Lett. 264, 12–19. doi: 10.1016/j.toxlet.2016.10.011

PubMed Abstract | Crossref Full Text | Google Scholar

Lapinlampi, N., Andrade, P., Paananen, T., Hamalainen, E., Ekolle Ndode-Ekane, X., Puhakka, N., et al. (2020). Postinjury weight rather than cognitive or behavioral impairment predicts development of posttraumatic epilepsy after lateral fluid-percussion injury in rats. Epilepsia 61, 2035–2052. doi: 10.1111/epi.16632

PubMed Abstract | Crossref Full Text | Google Scholar

Law, V., Knox, C., Djoumbou, Y., Jewison, T., Guo, A. C., Liu, Y., et al. (2014). DrugBank 4.0: shedding new light on drug metabolism. Nucleic Acids Res. 42(Database issue), D1091–D1097. doi: 10.1093/nar/gkt1068

PubMed Abstract | Crossref Full Text | Google Scholar

Leconte, C., Benedetto, C., Lentini, F., Simon, K., Ouaazizi, C., Taib, T., et al. (2020). Histological and behavioral evaluation after traumatic brain injury in mice: a ten months follow-up study. J. Neurotrauma 37, 1342–1357. doi: 10.1089/neu.2019.6679

PubMed Abstract | Crossref Full Text | Google Scholar

Lee, K. H., Cha, M., and Lee, B. H. (2020). Neuroprotective effect of antioxidants in the brain. Int. J. Mol. Sci. 21. doi: 10.3390/ijms21197152

PubMed Abstract | Crossref Full Text | Google Scholar

Lehman, C. W., Smith, A., Kelly, J., Jacobs, J. L., Dinman, J. D., Kehn-Hall, K., et al. (2022). EGR1 upregulation during encephalitic viral infections contributes to inflammation and cell death. Viruses 14:1210. doi: 10.3390/v14061210

PubMed Abstract | Crossref Full Text | Google Scholar

Lennette, E. H., and Koprowski, H. (1943). Human infection with venezuelan equine encephalomyelitis virus: a report on eight cases of infection acquired in the laboratory. J. Am. Med. Assoc. 123, 1088–1095. doi: 10.1001/jama.1943.02840520004002

Crossref Full Text | Google Scholar

Lenz, D. E., Maxwell, D. M., Koplovitz, I., Clark, C. R., Capacio, B. R., Cerasoli, D. M., et al. (2005). Protection against soman or VX poisoning by human butyrylcholinesterase in guinea pigs and cynomolgus monkeys. Chem. Biol. Interact. 157–158, 205–210. doi: 10.1016/j.cbi.2005.10.025

PubMed Abstract | Crossref Full Text | Google Scholar

Levi, M. S., and Brimble, M. A. (2004). A review of neuroprotective agents. Curr. Med. Chem. 11, 2383–2397. doi: 10.2174/0929867043364522

PubMed Abstract | Crossref Full Text | Google Scholar

Levin, H. S., and Hanten, G. (2005). Executive functions after traumatic brain injury in children. Pediatr. Neurol. 33, 79–93. doi: 10.1016/j.pediatrneurol.2005.02.002

PubMed Abstract | Crossref Full Text | Google Scholar

Levin, H. S., and Rodnitzky, R. L. (1976). Behavioral effects of organophosphate in man. Clin. Toxicol. 9, 391–403. doi: 10.3109/15563657608988138

PubMed Abstract | Crossref Full Text | Google Scholar

Levine, B., and Griffin, D. E. (1992). Persistence of viral RNA in mouse brains after recovery from acute alphavirus encephalitis. J. Virol. 66, 6429–6435. doi: 10.1128/jvi.66.11.6429-6435.1992

PubMed Abstract | Crossref Full Text | Google Scholar

Li, C., Chen, S., Siedhoff, H. R., Grant, D., Liu, P., Balderrama, A., et al. (2023). Low-intensity open-field blast exposure effects on neurovascular unit ultrastructure in mice. Acta Neuropathol Commun. 11:144. doi: 10.1186/s40478-023-01636-4

PubMed Abstract | Crossref Full Text | Google Scholar

Li, X. G., Du, J. H., Lu, Y., and Lin, X. J. (2019). Neuroprotective effects of rapamycin on spinal cord injury in rats by increasing autophagy and Akt signaling. Neural. Regen. Res. 14, 721–727. doi: 10.4103/1673-5374.247476

PubMed Abstract | Crossref Full Text | Google Scholar

Liao, M. F., Lu, K. T., Hsu, J. L., Lee, C. H., Cheng, M. Y., Ro, L. S., et al. (2022). The role of autophagy and apoptosis in neuropathic pain formation. Int. J. Mol. Sci. 23. doi: 10.3390/ijms23052685

PubMed Abstract | Crossref Full Text | Google Scholar

Lindsey, N. P., Martin, S. W., Staples, J. E., and Fischer, M. (2020). Notes from the field: multistate outbreak of eastern equine encephalitis virus - United States, 2019. MMWR Morb. Mortal. Wkly. Rep. 69, 50–51. doi: 10.15585/mmwr.mm6902a4

PubMed Abstract | Crossref Full Text | Google Scholar

Liu, Y. R., Cardamone, L., Hogan, R. E., Gregoire, M. C., Williams, J. P., Hicks, R. J., et al. (2010). Progressive metabolic and structural cerebral perturbations after traumatic brain injury: an in vivo imaging study in the rat. J. Nucl. Med. 51, 1788–1795. doi: 10.2967/jnumed.110.078626

PubMed Abstract | Crossref Full Text | Google Scholar

Loane, D. J., and Faden, A. I. (2010). Neuroprotection for traumatic brain injury: translational challenges and emerging therapeutic strategies. Trends Pharmacol. Sci. 31, 596–604. doi: 10.1016/j.tips.2010.09.005

PubMed Abstract | Crossref Full Text | Google Scholar

Loane, D. J., and Kumar, A. (2016). Microglia in the TBI brain: the good, the bad, and the dysregulated. Exp. Neurol. 275(Pt 3), 316–327. doi: 10.1016/j.expneurol.2015.08.018

PubMed Abstract | Crossref Full Text | Google Scholar

Logsdon, A. F., Meabon, J. S., Cline, M. M., Bullock, K. M., Raskind, M. A., Peskind, E. R., et al. (2018). Blast exposure elicits blood-brain barrier disruption and repair mediated by tight junction integrity and nitric oxide dependent processes. Sci. Rep. 8:11344. doi: 10.1038/s41598-018-29341-6

PubMed Abstract | Crossref Full Text | Google Scholar

London, W. T., Levitt, N. H., Kent, S. G., Wong, V. G., and Sever, J. L. (1977). Congenital cerebral and ocular malformations induced in rhesus monkeys by Venezuelan equine encephalitis virus. Teratology 16, 285–285. doi: 10.1002/tera.1420160307

PubMed Abstract | Crossref Full Text | Google Scholar

Loomis, T. A., and Johnson, D. D. (1966). Reversal of a soman-induced effect on neuromuscular function without reactivation of cholinesterase. Toxicol. Appl. Pharmacol. 8, 528–532. doi: 10.1016/0041-008X(66)90064-0

PubMed Abstract | Crossref Full Text | Google Scholar

Lopez-Munoz, F., Alamo, C., Guerra, J. A., and Garcia-Garcia, P. (2008). The development of neurotoxic agents as chemical weapons during the National Socialist period in Germany. Rev. Neurol. 47, 99–106.

PubMed Abstract | Google Scholar

Lu, C., Xia, J., Bin, W., Wu, Y., Liu, X., Zhang, Y., et al. (2015). Advances in diagnosis, treatments, and molecular mechanistic studies of traumatic brain injury. Biosci. Trends. 9, 138–148. doi: 10.5582/bst.2015.01066

PubMed Abstract | Crossref Full Text | Google Scholar

Lu, J., Ng, K. C., Ling, G., Wu, J., Poon, D. J., Kan, E. M., et al. (2012). Effect of blast exposure on the brain structure and cognition in Macaca fascicularis. J. Neurotrauma 29, 1434–1454. doi: 10.1089/neu.2010.1591

PubMed Abstract | Crossref Full Text | Google Scholar

Lucki, I., Dalvi, A., and Mayorga, A. J. (2001). Sensitivity to the effects of pharmacologically selective antidepressants in different strains of mice. Psychopharmacology 155, 315–322. doi: 10.1007/s002130100694

PubMed Abstract | Crossref Full Text | Google Scholar

Ludwig, G. V., Turell, M. J., Vogel, P., Kondig, J. P., Kell, W. K., Smith, J. F., et al. (2001). Comparative neurovirulence of attenuated and non-attenuated strains of Venezuelan equine encephalitis virus in mice. Am. J. Trop. Med. Hyg. 64, 49–55. doi: 10.4269/ajtmh.2001.64.49

PubMed Abstract | Crossref Full Text | Google Scholar

Luethy, D. (2023). Eastern, western, and venezuelan equine encephalitis and west nile viruses: clinical and public health considerations. Vet. Clin. North Am. Equine Pract. 39, 99–113. doi: 10.1016/j.cveq.2022.11.007

PubMed Abstract | Crossref Full Text | Google Scholar

Lundberg, L., Brahms, A., Hooper, I., Carey, B., Lin, S. C., Dahal, B., et al. (2018). Repurposed FDA-Approved drug sorafenib reduces replication of Venezuelan equine encephalitis virus and other alphaviruses. Antiviral Res. 157, 57–67. doi: 10.1016/j.antiviral.2018.07.005

PubMed Abstract | Crossref Full Text | Google Scholar

Lundberg, L., Carey, B., and Kehn-Hall, K. (2017). Venezuelan equine encephalitis virus capsid-the Clever Caper. Viruses 9:3126. doi: 10.3389/fmicb.2018.03126

PubMed Abstract | Crossref Full Text | Google Scholar

Luo, J., Nguyen, A., Villeda, S., Zhang, H., Ding, Z., Lindsey, D., et al. (2014). Long-term cognitive impairments and pathological alterations in a mouse model of repetitive mild traumatic brain injury. Front. Neurol. 5:12. doi: 10.3389/fneur.2014.00012

PubMed Abstract | Crossref Full Text | Google Scholar

Ma, H., Albe, J. R., Gilliland, T., McMillen, C. M., Gardner, C. L., Boyles, D. A., et al. (2022). Long-term persistence of viral RNA and inflammation in the CNS of macaques exposed to aerosolized Venezuelan equine encephalitis virus. PLoS Pathog. 18:e1009946. doi: 10.1371/journal.ppat.1009946

PubMed Abstract | Crossref Full Text | Google Scholar

Ma, X., Aravind, A., Pfister, B. J., Chandra, N., and Haorah, J. (2019). Animal models of traumatic brain injury and assessment of injury severity. Mol. Neurobiol. 56, 5332–5345. doi: 10.1007/s12035-018-1454-5

PubMed Abstract | Crossref Full Text | Google Scholar

Malkesman, O., Tucker, L. B., Ozl, J., and McCabe, J. T. (2013). Traumatic brain injury - modeling neuropsychiatric symptoms in rodents. Front. Neurol. 4:157. doi: 10.3389/fneur.2013.00157

PubMed Abstract | Crossref Full Text | Google Scholar

Mallah, K., Couch, C., Borucki, D. M., Toutonji, A., Alshareef, M., Tomlinson, S., et al. (2020). Anti-inflammatory and neuroprotective agents in clinical trials for CNS disease and injury: where do we go from here? Front. Immunol. 11:2021. doi: 10.3389/fimmu.2020.02021

PubMed Abstract | Crossref Full Text | Google Scholar

Mamczarz, J., Pereira, E. F., Aracava, Y., Adler, M., and Albuquerque, E. X. (2010). An acute exposure to a sub-lethal dose of soman triggers anxiety-related behavior in guinea pigs: interactions with acute restraint. Neurotoxicology 31, 77–84. doi: 10.1016/j.neuro.2009.10.012

PubMed Abstract | Crossref Full Text | Google Scholar

Mao, X., Terpolilli, N. A., Wehn, A., Cheng, S., Hellal, F., Liu, B., et al. (2020). Progressive histopathological damage occurring up to one year after experimental traumatic brain injury is associated with cognitive decline and depression-like behavior. J. Neurotrauma 37, 1331–1341. doi: 10.1089/neu.2019.6510

PubMed Abstract | Crossref Full Text | Google Scholar

Marrero-Rosado, B., Rossetti, F., Rice, M. W., Moffett, M. C., Lee, R. B., Stone, M. F., et al. (2018). Age-related susceptibility to epileptogenesis and neuronal loss in male fischer rats exposed to soman and treated with medical countermeasures. Toxicol. Sci. 164, 142–152. doi: 10.1093/toxsci/kfy065

PubMed Abstract | Crossref Full Text | Google Scholar

Marrero-Rosado, B. M., Stone, M. F., de Araujo Furtado, M., Schultz, C. R., Cadieux, C. L., Lumley, L. A., et al. (2021). Novel genetically modified mouse model to assess soman-induced toxicity and medical countermeasure efficacy: human acetylcholinesterase knock-in serum carboxylesterase knockout mice. Int. J. Mol. Sci. 22. doi: 10.3390/ijms22041893

PubMed Abstract | Crossref Full Text | Google Scholar

Matson, L. M., McCarren, H. S., Cadieux, C. L., Cerasoli, D. M., and McDonough, J. H. (2018). The role of genetic background in susceptibility to chemical warfare nerve agents across rodent and non-human primate models. Toxicology 393, 51–61. doi: 10.1016/j.tox.2017.11.003

PubMed Abstract | Crossref Full Text | Google Scholar

Maxwell, D. M., Castro, C. A., De La Hoz, D. M., Gentry, M. K., Gold, M. B., Solana, R. P., et al. (1992). Protection of rhesus monkeys against soman and prevention of performance decrement by pretreatment with acetylcholinesterase. Toxicol. Appl. Pharmacol. 115, 44–49. doi: 10.1016/0041-008X(92)90365-Y

PubMed Abstract | Crossref Full Text | Google Scholar

McCarren, H. S., Eisen, M. R., Nguyen, D. L., Dubee, P. B., Ardinger, C. E., Dunn, E. N., et al. (2020). Characterization and treatment of spontaneous recurrent seizures following nerve agent-induced status epilepticus in mice. Epilepsy Res. 162:106320. doi: 10.1016/j.eplepsyres.2020.106320

PubMed Abstract | Crossref Full Text | Google Scholar

McCarthy, M. M. (2016). Multifaceted origins of sex differences in the brain. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 371:20150106. doi: 10.1098/rstb.2015.0106

PubMed Abstract | Crossref Full Text | Google Scholar

McGivern, J. G. (2007). Ziconotide: a review of its pharmacology and use in the treatment of pain. Neuropsychiatr. Dis. Treat. 3, 69–85. doi: 10.2147/nedt.2007.3.1.69

PubMed Abstract | Crossref Full Text | Google Scholar

McGowan, J. C., Yang, J. H., Plotkin, R. C., Grossman, R. I., Umile, E. M., Cecil, K. M., et al. (2000). Magnetization transfer imaging in the detection of injury associated with mild head trauma. AJNR Am. J. Neuroradiol. 21, 875–880.

Google Scholar

McKee, A. C., Cantu, R. C., Nowinski, C. J., Hedley-Whyte, E. T., Gavett, B. E., Budson, A. E., et al. (2009). Chronic traumatic encephalopathy in athletes: progressive tauopathy after repetitive head injury. J. Neuropathol. Exp. Neurol. 68, 709–735. doi: 10.1097/NEN.0b013e3181a9d503

PubMed Abstract | Crossref Full Text | Google Scholar

McKee, C. A., and Lukens, J. R. (2016). Emerging roles for the immune system in traumatic brain injury. Front. Immunol. 7:556. doi: 10.3389/fimmu.2016.00556

PubMed Abstract | Crossref Full Text | Google Scholar

McLeod, C. G. Jr. (1985). Pathology of nerve agents: perspectives on medical management. Fundam. Appl. Toxicol. 5, S10–S16. doi: 10.1016/0272-0590(85)90110-1

PubMed Abstract | Crossref Full Text | Google Scholar

McLeod, C. G. Jr., Singer, A. W., and Harrington, D. G. (1984). Acute neuropathology in soman poisoned rats. Neurotoxicology 5, 53–57.

Google Scholar

Miller, G. F., Kegler, S. R., and Stone, D. M. (2020). Traumatic brain injury-related deaths from firearm suicide: United States, 2008-2017. Am. J. Public Health 110, 897–899. doi: 10.2105/AJPH.2020.305622

PubMed Abstract | Crossref Full Text | Google Scholar

Miller, S. L., Aroniadou-Anderjaska, V., Figueiredo, T. H., Prager, E. M., Almeida-Suhett, C. P., Apland, J. P., et al. (2015). A rat model of nerve agent exposure applicable to the pediatric population: the anticonvulsant efficacies of atropine and GluK1 antagonists. Toxicol. Appl. Pharmacol. 284, 204–216. doi: 10.1016/j.taap.2015.02.008

PubMed Abstract | Crossref Full Text | Google Scholar

Milleville, K. A., Awan, N., Disanto, D., Kumar, R. G., and Wagner, A. K. (2021). Early chronic systemic inflammation and associations with cognitive performance after moderate to severe TBI. Brain Behav. Immun. Health 11:100185. doi: 10.1016/j.bbih.2020.100185

PubMed Abstract | Crossref Full Text | Google Scholar

Mogil, J. S., Wilson, S. G., Bon, K., Lee, S. E., Chung, K., Raber, P., et al. (1999). Heritability of nociception I: responses of 11 inbred mouse strains on 12 measures of nociception. Pain 80, 67–82. doi: 10.1016/S0304-3959(98)00197-3

PubMed Abstract | Crossref Full Text | Google Scholar

Montiel, M., Bonilla, E., Valero, N., Mosquera, J., Espina, L. M., Quiroz, Y., et al. (2015). Melatonin decreases brain apoptosis, oxidative stress, and CD200 expression and increased survival rate in mice infected by Venezuelan equine encephalitis virus. Antivir. Chem. Chemother. 24, 99–108. doi: 10.1177/2040206616660851

PubMed Abstract | Crossref Full Text | Google Scholar

Morita, H., Yanagisawa, N., Nakajima, T., Shimizu, M., Hirabayashi, H., Okudera, H., et al. (1995). Sarin poisoning in Matsumoto, Japan. Lancet 346, 290–293. doi: 10.1016/S0140-6736(95)92170-2

PubMed Abstract | Crossref Full Text | Google Scholar

Moshiri, M., Darchini-Maragheh, E., and Balali-Mood, M. (2012). Advances in toxicology and medical treatment of chemical warfare nerve agents. Daru 20:81. doi: 10.1186/2008-2231-20-81

PubMed Abstract | Crossref Full Text | Google Scholar

Mouzon, B., Chaytow, H., Crynen, G., Bachmeier, C., Stewart, J., Mullan, M., et al. (2012). Repetitive mild traumatic brain injury in a mouse model produces learning and memory deficits accompanied by histological changes. J. Neurotrauma 29, 2761–2773. doi: 10.1089/neu.2012.2498

PubMed Abstract | Crossref Full Text | Google Scholar

Mukherjee, S., and Gupta, R. D. (2020). Organophosphorus nerve agents: types, toxicity, and treatments. J. Toxicol. 2020:3007984. doi: 10.1155/2020/3007984

PubMed Abstract | Crossref Full Text | Google Scholar

Mulder, D. W., Parrott, M., and Thaler, M. (1951). Sequelae of western equine encephalitis. Neurology 1, 318–327. doi: 10.1212/WNL.1.7-8.318

PubMed Abstract | Crossref Full Text | Google Scholar

Munivenkatappa, A., Agrawal, A., Shukla, D. P., Kumaraswamy, D., and Devi, B. I. (2016). Traumatic brain injury: does gender influence outcomes? Int. J. Crit. Illn. Inj. Sci. 6, 70–73. doi: 10.4103/2229-5151.183024

PubMed Abstract | Crossref Full Text | Google Scholar

Myhrer, T., Andersen, J. M., Nguyen, N. H., and Aas, P. (2005). Soman-induced convulsions in rats terminated with pharmacological agents after 45 min: neuropathology and cognitive performance. Neurotoxicology 26, 39–48. doi: 10.1016/j.neuro.2004.07.011

PubMed Abstract | Crossref Full Text | Google Scholar

Myhrer, T., Enger, S., and Aas, P. (2008). Antiparkinson drugs used as prophylactics for nerve agents: studies of cognitive side effects in rats. Pharmacol. Biochem. Behav. 89, 633–638. doi: 10.1016/j.pbb.2008.02.022

PubMed Abstract | Crossref Full Text | Google Scholar

Myhrer, T., Enger, S., and Aas, P. (2013). The perirhinal cortex of rats: an intricate area for microinfusion of anticonvulsants against soman-induced seizures. Neurotoxicology 34, 128–134. doi: 10.1016/j.neuro.2012.10.019

PubMed Abstract | Crossref Full Text | Google Scholar

Nakajima, T., Ohta, S., Fukushima, Y., and Yanagisawa, N. (1999). Sequelae of sarin toxicity at one and three years after exposure in Matsumoto, Japan. J. Epidemiol. 9, 337–343. doi: 10.2188/jea.9.337

PubMed Abstract | Crossref Full Text | Google Scholar

National Research Council (US) Subcommittee on Chronic Reference Doses for Selected Chemical Warfare Agents (1999). Review of the U.S. Army's Health Risk Assessments For Oral Exposure to Six Chemical-Warfare Agents. Washington, DC: National Academies Press.

Google Scholar

Nelson, C. G., Elta, T., Bannister, J., Dzandu, J., Mangram, A., Zach, V., et al. (2016). Severe traumatic brain injury: a case report. Am. J. Case Rep. 17, 186–191. doi: 10.12659/AJCR.897116

PubMed Abstract | Crossref Full Text | Google Scholar

Nieminen, S. A., Lecklin, A., Heikkinen, O., and Ylitalo, P. (1990). Acute behavioural effects of the organophosphates sarin and soman in rats. Pharmacol. Toxicol. 67, 36–40. doi: 10.1111/j.1600-0773.1990.tb00778.x

PubMed Abstract | Crossref Full Text | Google Scholar

NINDS (2024). Traumatic Brain Injury. National Institute of Neurological Disorders and Stroke. Available at: https://www.ninds.nih.gov/health-information/disorders/traumatic-brain-injury-tbi (accessed November 1, 2024).

Google Scholar

Nishimura, K., Cordeiro, J. G., Ahmed, A. I., Yokobori, S., and Gajavelli, S. (2022). Advances in traumatic brain injury biomarkers. Cureus 14:e23804. doi: 10.7759/cureus.23804

PubMed Abstract | Crossref Full Text | Google Scholar

Noga, M., and Jurowski, K. (2023). What do we currently know about Novichoks? The state of the art. Arch. Toxicol. 97, 651–661. doi: 10.1007/s00204-022-03437-5

PubMed Abstract | Crossref Full Text | Google Scholar

Nordstrom, A., Edin, B. B., Lindstrom, S., and Nordstrom, P. (2013). Cognitive function and other risk factors for mild traumatic brain injury in young men: nationwide cohort study. BMJ 346:f723. doi: 10.1136/bmj.f723

PubMed Abstract | Crossref Full Text | Google Scholar

Nozaki, H., Aikawa, N., Fujishima, S., Suzuki, M., Shinozawa, Y., Hori, S., et al. (1995). A case of VX poisoning and the difference from sarin. Lancet 346, 698–699. doi: 10.1016/S0140-6736(95)92306-3

Crossref Full Text | Google Scholar

NRC (1997). Review of Acute Human-Toxicity Estimates for Selected Chemical-Warfare Agents. Washington, DC: NRC.

PubMed Abstract | Google Scholar

O'Donnell, J. C., McDonough, J. H., and Shih, T. M. (2011). In vivo microdialysis and electroencephalographic activity in freely moving guinea pigs exposed to organophosphorus nerve agents sarin and VX: analysis of acetylcholine and glutamate. Arch. Toxicol. 85, 1607–1616. doi: 10.1007/s00204-011-0724-z

PubMed Abstract | Crossref Full Text | Google Scholar

Oertel, W., and Schulz, J. B. (2016). Current and experimental treatments of Parkinson disease: a guide for neuroscientists. J. Neurochem. 139(Suppl)1, 325–337. doi: 10.1111/jnc.13750

PubMed Abstract | Crossref Full Text | Google Scholar

Ogorek, T. J., and Golden, J. E. (2023). Advances in the development of small molecule antivirals against equine encephalitic viruses. Viruses 15:413. doi: 10.3390/v15020413

PubMed Abstract | Crossref Full Text | Google Scholar

Okumura, T., Hisaoka, T., Naito, T., Isonuma, H., Okumura, S., Miura, K., et al. (2005). Acute and chronic effects of sarin exposure from the Tokyo subway incident. Environ. Toxicol. Pharmacol. 19, 447–450. doi: 10.1016/j.etap.2004.12.005

PubMed Abstract | Crossref Full Text | Google Scholar

Okumura, T., Takasu, N., Ishimatsu, S., Miyanoki, S., Mitsuhashi, A., Kumada, K., et al. (1996). Report on 640 victims of the Tokyo subway sarin attack. Ann. Emerg. Med. 28, 129–135. doi: 10.1016/S0196-0644(96)70052-5

PubMed Abstract | Crossref Full Text | Google Scholar

Olczak, M., Kwiatkowska, M., Niderla-Bielinska, J., Chutoranski, D., Tarka, S., Wierzba-Bobrowicz, T., et al. (2018). Brain-originated peptides as possible biochemical markers of traumatic brain injury in cerebrospinal fluid post-mortem examination. Folia Neuropathol. 56, 97–103. doi: 10.5114/fn.2018.76613

PubMed Abstract | Crossref Full Text | Google Scholar

Omalu, B. I., DeKosky, S. T., Hamilton, R. L., Minster, R. L., Kamboh, M. I., Shakir, A. M., et al. (2006). Chronic traumatic encephalopathy in a national football league player: part II. Neurosurgery 59, 1086–1092. discussion 92–93. doi: 10.1227/01.NEU.0000245601.69451.27

PubMed Abstract | Crossref Full Text | Google Scholar

Omalu, B. I., DeKosky, S. T., Minster, R. L., Kamboh, M. I., Hamilton, R. L., Wecht, C. H., et al. (2005). Chronic traumatic encephalopathy in a National Football League player. Neurosurgery 57, 128–134. discussion−34. doi: 10.1227/01.NEU.0000163407.92769.ED

PubMed Abstract | Crossref Full Text | Google Scholar

Opravil, J., Pejchal, J., Finger, V., Korabecny, J., Rozsypal, T., Hrabinova, M., et al. (2023). A-agents, misleadingly known as “Novichoks”: a narrative review. Arch. Toxicol. 97, 2587–2607. doi: 10.1007/s00204-023-03571-8

PubMed Abstract | Crossref Full Text | Google Scholar

Osier, N. D., Carlson, S. W., DeSana, A., and Dixon, C. E. (2015). Chronic histopathological and behavioral outcomes of experimental traumatic brain injury in adult male animals. J. Neurotrauma 32, 1861–1882. doi: 10.1089/neu.2014.3680

PubMed Abstract | Crossref Full Text | Google Scholar

Oswal, D. P., Garrett, T. L., Morris, M., and Lucot, J. B. (2013). Low-dose sarin exposure produces long term changes in brain neurochemistry of mice. Neurochem. Res. 38, 108–116. doi: 10.1007/s11064-012-0896-9

PubMed Abstract | Crossref Full Text | Google Scholar

Paessler, S., Ni, H., Petrakova, O., Fayzulin, R. Z., Yun, N., Anishchenko, M., et al. (2006). Replication and clearance of Venezuelan equine encephalitis virus from the brains of animals vaccinated with chimeric SIN/VEE viruses. J. Virol. 80, 2784–2796. doi: 10.1128/JVI.80.6.2784-2796.2006

PubMed Abstract | Crossref Full Text | Google Scholar

Palmer, C. P., Metheny, H. E., Elkind, J. A., and Cohen, A. S. (2016). Diminished amygdala activation and behavioral threat response following traumatic brain injury. Exp. Neurol. 277, 215–226. doi: 10.1016/j.expneurol.2016.01.004

PubMed Abstract | Crossref Full Text | Google Scholar

Palmer, R. J., and Finley, K. H. (1956). Sequelae of encephalitis; report of a study after the California epidemic. Calif. Med. 84, 98–100.

Google Scholar

Pandey, D. K., Yadav, S. K., Mahesh, R., and Rajkumar, R. (2009). Depression-like and anxiety-like behavioural aftermaths of impact accelerated traumatic brain injury in rats: a model of comorbid depression and anxiety? Behav. Brain Res. 205, 436–442. doi: 10.1016/j.bbr.2009.07.027

PubMed Abstract | Crossref Full Text | Google Scholar

Panny, L., Akrhymuk, I., Bracci, N., Woodson, C., Flor, R., Elliott, I., et al. (2023). Venezuelan equine encephalitis virus E1 protein interacts with PDIA6 and PDI inhibition reduces alphavirus production. Antiviral Res. 212:105560. doi: 10.1016/j.antiviral.2023.105560

PubMed Abstract | Crossref Full Text | Google Scholar

Pardo-Moreno, T., Garcia-Morales, V., Suleiman-Martos, S., Rivas-Dominguez, A., Mohamed-Mohamed, H., Ramos-Rodriguez, J. J., et al. (2023). Current treatments and new, tentative therapies for Parkinson's disease. Pharmaceutics 15:770. doi: 10.3390/pharmaceutics15030770

PubMed Abstract | Crossref Full Text | Google Scholar

Patel, S., Maria-Rios, J., Parikh, A., and Okorie, O. N. (2023). Diagnosis and management of elevated intracranial pressure in the emergency department. Int. J. Emerg. Med. 16:72. doi: 10.1186/s12245-023-00540-x

PubMed Abstract | Crossref Full Text | Google Scholar

Paterno, R., Folweiler, K. A., and Cohen, A. S. (2017). Pathophysiology and treatment of memory dysfunction after traumatic brain injury. Curr. Neurol. Neurosci. Rep. 17:52. doi: 10.1007/s11910-017-0762-x

PubMed Abstract | Crossref Full Text | Google Scholar

Pazdernik, T. L., Emerson, M. R., Cross, R., Nelson, S. R., and Samson, F. E. (2001). Soman-induced seizures: limbic activity, oxidative stress and neuroprotective proteins. J. Appl. Toxicol. 21(Suppl 1), S87–S94. doi: 10.1002/jat.818

PubMed Abstract | Crossref Full Text | Google Scholar

Pearson, J. N., and Patel, M. (2016). The role of oxidative stress in organophosphate and nerve agent toxicity. Ann. N. Y. Acad. Sci. 1378, 17–24. doi: 10.1111/nyas.13115

PubMed Abstract | Crossref Full Text | Google Scholar

Pearson-Smith, J. N., Liang, L. P., Rowley, S. D., Day, B. J., and Patel, M. (2017). Oxidative stress contributes to status epilepticus associated mortality. Neurochem. Res. 42, 2024–2032. doi: 10.1007/s11064-017-2273-1

PubMed Abstract | Crossref Full Text | Google Scholar

Pearson-Smith, J. N., and Patel, M. (2020). Antioxidant drug therapy as a neuroprotective countermeasure of nerve agent toxicity. Neurobiol. Dis. 133:104457. doi: 10.1016/j.nbd.2019.04.013

PubMed Abstract | Crossref Full Text | Google Scholar

Peiseler, M., and Kubes, P. (2019). More friend than foe: the emerging role of neutrophils in tissue repair. J. Clin. Invest. 129, 2629–2639. doi: 10.1172/JCI124616

PubMed Abstract | Crossref Full Text | Google Scholar

Peng, B. H., Borisevich, V., Popov, V. L., Zacks, M. A., Estes, D. M., Campbell, G. A., et al. (2013). Production of IL-8, IL-17, IFN-gamma and IP-10 in human astrocytes correlates with alphavirus attenuation. Vet. Microbiol. 163, 223–234. doi: 10.1016/j.vetmic.2012.11.021

PubMed Abstract | Crossref Full Text | Google Scholar

Pereira, E. F., Aracava, Y., DeTolla, L. J. Jr., Beecham, E. J., Basinger, G. W. Jr., Wakayama, E. J., et al. (2014). Animal models that best reproduce the clinical manifestations of human intoxication with organophosphorus compounds. J. Pharmacol. Exp. Ther. 350, 313–321. doi: 10.1124/jpet.114.214932

PubMed Abstract | Crossref Full Text | Google Scholar

Peterson, A. B., Zhou, H., and Thomas, K. E. (2022). Disparities in traumatic brain injury-related deaths-United States, 2020. J. Safety Res. 83, 419–426. doi: 10.1016/j.jsr.2022.10.001

PubMed Abstract | Crossref Full Text | Google Scholar

Petras, J. M. (1994). Neurology and neuropathology of Soman-induced brain injury: an overview. J. Exp. Anal. Behav. 61, 319–329. doi: 10.1901/jeab.1994.61-319

PubMed Abstract | Crossref Full Text | Google Scholar

Phelps, A. L., O'Brien, L. M., Eastaugh, L. S., Davies, C., Lever, M. S., Ennis, J., et al. (2017). Susceptibility and lethality of western equine encephalitis virus in Balb/c Mice when infected by the aerosol route. Viruses 9:163. doi: 10.3390/v9070163

PubMed Abstract | Crossref Full Text | Google Scholar

Phelps, A. L., O'Brien, L. M., Ulaeto, D. O., Holtsberg, F. W., Liao, G. C., Douglas, R., et al. (2021). Cross-strain neutralizing and protective monoclonal antibodies against EEEV or WEEV. Viruses 13:2231. doi: 10.3390/v13112231

PubMed Abstract | Crossref Full Text | Google Scholar

Phelps, A. L., Salguero, F. J., Hunter, L., Stoll, A. L., Jenner, D. C., O'Brien, L. M., et al. (2023). Tumour necrosis factor-alpha, chemokines, and leukocyte infiltrate are biomarkers for pathology in the brains of venezuelan equine encephalitis (VEEV)-infected mice. Viruses 15:1307. doi: 10.3390/v15061307

PubMed Abstract | Crossref Full Text | Google Scholar

Phillips, A. T., Rico, A. B., Stauft, C. B., Hammond, S. L., Aboellail, T. A., Tjalkens, R. B., et al. (2016). Entry sites of venezuelan and western equine encephalitis viruses in the mouse central nervous system following peripheral infection. J. Virol. 90, 5785–5796. doi: 10.1128/JVI.03219-15

PubMed Abstract | Crossref Full Text | Google Scholar

Piekut, T., Hurla, M., Banaszek, N., Szejn, P., Dorszewska, J., Kozubski, W., et al. (2022). Infectious agents and Alzheimer's disease. J. Integr. Neurosci. 21:73. doi: 10.31083/j.jin2102073

PubMed Abstract | Crossref Full Text | Google Scholar

Pischiutta, F., Micotti, E., Hay, J. R., Marongiu, I., Sammali, E., Tolomeo, D., et al. (2018). Single severe traumatic brain injury produces progressive pathology with ongoing contralateral white matter damage one year after injury. Exp. Neurol. 300, 167–178. doi: 10.1016/j.expneurol.2017.11.003

PubMed Abstract | Crossref Full Text | Google Scholar

Porter, A. I., Erwin-Cohen, R. A., Twenhafel, N., Chance, T., Yee, S. B., Kern, S. J., et al. (2017). Characterization and pathogenesis of aerosolized eastern equine encephalitis in the common marmoset (Callithrix jacchus). Virol. J. 14:25. doi: 10.1186/s12985-017-0687-7

PubMed Abstract | Crossref Full Text | Google Scholar

Prager, E. M., Pidoplichko, V. I., Aroniadou-Anderjaska, V., Apland, J. P., and Braga, M. F. (2014). Pathophysiological mechanisms underlying increased anxiety after soman exposure: reduced GABAergic inhibition in the basolateral amygdala. Neurotoxicology 44, 335–343. doi: 10.1016/j.neuro.2014.08.007

PubMed Abstract | Crossref Full Text | Google Scholar

Radic, B., Vrdoljak, A. L., Petek, M. J., Kopjar, N., and Zeljezic, D. (2006). In vitro biological efficiency of tenocyclidine-TCP and its adamantane derivative TAMORF. Toxicol. In Vitro. 20, 1455–1464. doi: 10.1016/j.tiv.2006.07.002

PubMed Abstract | Crossref Full Text | Google Scholar

Ramos-Cejudo, J., Wisniewski, T., Marmar, C., Zetterberg, H., Blennow, K., de Leon, M. J., et al. (2018). Traumatic brain injury and Alzheimer's disease: the cerebrovascular link. EBioMed. 28, 21–30. doi: 10.1016/j.ebiom.2018.01.021

PubMed Abstract | Crossref Full Text | Google Scholar

Rapoport, M. J. (2012). Depression following traumatic brain injury: epidemiology, risk factors and management. CNS Drugs 26, 111–121. doi: 10.2165/11599560-000000000-00000

PubMed Abstract | Crossref Full Text | Google Scholar

Raveh, L., Grauer, E., Grunwald, J., Cohen, E., and Ashani, Y. (1997). The stoichiometry of protection against soman and VX toxicity in monkeys pretreated with human butyrylcholinesterase. Toxicol. Appl. Pharmacol. 145, 43–53. doi: 10.1006/taap.1997.8160

PubMed Abstract | Crossref Full Text | Google Scholar

Raveh, L., Weissman, B. A., Cohen, G., Alkalay, D., Rabinovitz, I., Sonego, H., et al. (2002). Caramiphen and scopolamine prevent soman-induced brain damage and cognitive dysfunction. Neurotoxicology 23, 7–17. doi: 10.1016/S0161-813X(02)00005-0

PubMed Abstract | Crossref Full Text | Google Scholar

Reddy, A. J., Woods, C. W., and Welty-Wolf, K. E. (2008). Eastern equine encephalitis leading to multi-organ failure and sepsis. J. Clin. Virol. 42, 418–421. doi: 10.1016/j.jcv.2008.03.008

PubMed Abstract | Crossref Full Text | Google Scholar

Reddy, D. S. (2024). Neurosteroids as novel anticonvulsants for refractory status epilepticus and medical countermeasures for nerve agents: a 15-year journey to bring ganaxolone from bench to clinic. J. Pharmacol. Exp. Ther. 388, 273–300. doi: 10.1124/jpet.123.001816

PubMed Abstract | Crossref Full Text | Google Scholar

Reddy, S. D., Wu, X., Kuruba, R., Sridhar, V., and Reddy, D. S. (2020). Magnetic resonance imaging analysis of long-term neuropathology after exposure to the nerve agent soman: correlation with histopathology and neurological dysfunction. Ann. N. Y. Acad. Sci. 1480, 116–135. doi: 10.1111/nyas.14431

PubMed Abstract | Crossref Full Text | Google Scholar

Redell, J. B., Maynard, M. E., Underwood, E. L., Vita, S. M., Dash, P. K., Kobori, N., et al. (2020). Traumatic brain injury and hippocampal neurogenesis: functional implications. Exp. Neurol. 331:113372. doi: 10.1016/j.expneurol.2020.113372

PubMed Abstract | Crossref Full Text | Google Scholar

Reed, D. S., Larsen, T., Sullivan, L. J., Lind, C. M., Lackemeyer, M. G., Pratt, W. D., et al. (2005). Aerosol exposure to western equine encephalitis virus causes fever and encephalitis in cynomolgus macaques. J. Infect. Dis. 192, 1173–1182. doi: 10.1086/444397

PubMed Abstract | Crossref Full Text | Google Scholar

Reed, D. S., Lind, C. M., Sullivan, L. J., Pratt, W. D., and Parker, M. D. (2004). Aerosol infection of cynomolgus macaques with enzootic strains of venezuelan equine encephalitis viruses. J. Infect. Dis. 189, 1013–1017. doi: 10.1086/382281

PubMed Abstract | Crossref Full Text | Google Scholar

Rehman, M. U., Wali, A. F., Ahmad, A., Shakeel, S., Rasool, S., Ali, R., et al. (2019). Neuroprotective strategies for neurological disorders by natural products: an update. Curr. Neuropharmacol. 17, 247–267. doi: 10.2174/1570159X16666180911124605

PubMed Abstract | Crossref Full Text | Google Scholar

Remes, O., Mendes, J. F., and Templeton, P. (2021). Biological, Psychological, and social determinants of depression: a review of recent literature. Brain Sci. 11:1633. doi: 10.3390/brainsci11121633

PubMed Abstract | Crossref Full Text | Google Scholar

Ricci, M., Cimini, A., Camedda, R., Chiaravalloti, A., and Schillaci, O. (2021). Tau biomarkers in dementia: positron emission tomography radiopharmaceuticals in tauopathy assessment and future perspective. Int. J. Mol. Sci. 22:13002. doi: 10.3390/ijms222313002

PubMed Abstract | Crossref Full Text | Google Scholar

Risner, K., Ahmed, A., Bakovic, A., Kortchak, S., Bhalla, N., Narayanan, A., et al. (2019). Efficacy of FDA-approved anti-inflammatory drugs against venezuelan equine encephalitis virus infection. Viruses 11:1151. doi: 10.3390/v11121151

PubMed Abstract | Crossref Full Text | Google Scholar

Robertson, G. S., Crocker, S. J., Nicholson, D. W., and Schulz, J. B. (2000). Neuroprotection by the inhibition of apoptosis. Brain Pathol. 10, 283–292. doi: 10.1111/j.1750-3639.2000.tb00262.x

PubMed Abstract | Crossref Full Text | Google Scholar

Robinson, S., Berglass, J. B., Denson, J. L., Berkner, J., Anstine, C. V., Winer, J. L., et al. (2017). Microstructural and microglial changes after repetitive mild traumatic brain injury in mice. J. Neurosci. Res. 95, 1025–1105. doi: 10.1002/jnr.23848

PubMed Abstract | Crossref Full Text | Google Scholar

Rogers, D. C., Fisher, E. M., Brown, S. D., Peters, J., Hunter, A. J., Martin, J. E., et al. (1997). Behavioral and functional analysis of mouse phenotype: SHIRPA, a proposed protocol for comprehensive phenotype assessment. Mamm. Genome. 8, 711–713. doi: 10.1007/s003359900551

PubMed Abstract | Crossref Full Text | Google Scholar

Roldan-Tapia, L., Parron, T., and Sanchez-Santed, F. (2005). Neuropsychological effects of long-term exposure to organophosphate pesticides. Neurotoxicol. Teratol. 27, 259–266. doi: 10.1016/j.ntt.2004.12.002

PubMed Abstract | Crossref Full Text | Google Scholar

Ronca, S. E., Dineley, K. T., and Paessler, S. (2016). Neurological sequelae resulting from encephalitic alphavirus infection. Front. Microbiol. 7:959. doi: 10.3389/fmicb.2016.00959

PubMed Abstract | Crossref Full Text | Google Scholar

Ronca, S. E., Smith, J., Koma, T., Miller, M. M., Yun, N., Dineley, K. T., et al. (2017). Mouse model of neurological complications resulting from encephalitic alphavirus infection. Front. Microbiol. 8:188. doi: 10.3389/fmicb.2017.00188

PubMed Abstract | Crossref Full Text | Google Scholar

Rosenblatt, D. H., Small, M. J., Kimmell, T. A., and Anderson, A. W. (1996). Background chemistry for chemical warfare agents and decontamination processes in support of delisting waste streams at the U.S. Army Dugway Proving Ground, Utah. Oak Ridge, TN: Department of Energy's (DOE) OSTIGOV. doi: 10.2172/258187

Crossref Full Text | Google Scholar

Rosenstock, L., Keifer, M., Daniell, W. E., McConnell, R., and Claypoole, K. (1991). Chronic central nervous system effects of acute organophosphate pesticide intoxication. The Pesticide Health Effects Study Group. Lancet 338, 223–227. doi: 10.1016/0140-6736(91)90356-T

PubMed Abstract | Crossref Full Text | Google Scholar

Rosman, Y., Eisenkraft, A., Milk, N., Shiyovich, A., Ophir, N., Shrot, S., et al. (2014). Lessons learned from the Syrian sarin attack: evaluation of a clinical syndrome through social media. Ann. Intern. Med. 160, 644–648. doi: 10.7326/M13-2799

PubMed Abstract | Crossref Full Text | Google Scholar

Rotenberg, J. S., and Newmark, J. (2003). Nerve agent attacks on children: diagnosis and management. Pediatrics 112, 648–658. doi: 10.1542/peds.112.3.648

PubMed Abstract | Crossref Full Text | Google Scholar

Rusnak, J. M., Dupuy, L. C., Niemuth, N. A., Glenn, A. M., and Ward, L. A. (2018). Comparison of aerosol- and percutaneous-acquired venezuelan equine encephalitis in humans and nonhuman primates for suitability in predicting clinical efficacy under the animal rule. Comp. Med. 68, 380–395. doi: 10.30802/AALAS-CM-18-000027

PubMed Abstract | Crossref Full Text | Google Scholar

Rusnak, J. M., Glass, P. J., Weaver, S. C., Sabourin, C. L., Glenn, A. M., Klimstra, W., et al. (2019). Approach to strain selection and the propagation of viral stocks for venezuelan equine encephalitis virus vaccine efficacy testing under the animal rule. Viruses 11:807. doi: 10.3390/v11090807

PubMed Abstract | Crossref Full Text | Google Scholar

Saikh, K. U., Morazzani, E. M., Piper, A. E., Bakken, R. R., and Glass, P. J. (2020). A small molecule inhibitor of MyD88 exhibits broad spectrum antiviral activity by up regulation of type I interferon. Antiviral Res. 181:104854. doi: 10.1016/j.antiviral.2020.104854

PubMed Abstract | Crossref Full Text | Google Scholar

Salimi, H., Cain, M. D., Jiang, X., Roth, R. A., Beatty, W. L., Sun, C., et al. (2020). Encephalitic alphaviruses exploit caveola-mediated transcytosis at the blood-brain barrier for central nervous system entry. mBio 11:e02731-19. doi: 10.1128/mBio.02731-19

PubMed Abstract | Crossref Full Text | Google Scholar

Sarkar, S., Krishna, G., Imarisio, S., Saiki, S., O'Kane, C. J., Rubinsztein, D. C. A., et al. (2008). rational mechanism for combination treatment of Huntington's disease using lithium and rapamycin. Hum. Mol. Genet. 17, 170–178. doi: 10.1093/hmg/ddm294

PubMed Abstract | Crossref Full Text | Google Scholar

Sattur, M. G., Patel, S. J., Helke, K. L., Donohoe, M., and Spiotta, A. M. (2023). Head elevation, cerebral venous system, and intracranial pressure: review and hypothesis. Stroke-Vasc. Inter. Neurol. 3. doi: 10.1161/SVIN.122.000522

Crossref Full Text | Google Scholar

Savage, E. P., Keefe, T. J., Mounce, L. M., Heaton, R. K., Lewis, J. A., Burcar, P. J., et al. (1988). Chronic neurological sequelae of acute organophosphate pesticide poisoning. Arch. Environ. Health 43, 38–45. doi: 10.1080/00039896.1988.9934372

PubMed Abstract | Crossref Full Text | Google Scholar

Saw, M. M., Chamberlain, J., Barr, M., Morgan, M. P. G., Burnett, J. R., Ho, K. M., et al. (2014). Differential disruption of blood-brain barrier in severe traumatic brain injury. Neurocrit. Care. 20, 209–216. doi: 10.1007/s12028-013-9933-z

PubMed Abstract | Crossref Full Text | Google Scholar

Schmidt, D. (2009). Drug treatment of epilepsy: options and limitations. Epilepsy Behav. 15, 56–65. doi: 10.1016/j.yebeh.2009.02.030

PubMed Abstract | Crossref Full Text | Google Scholar

Schoepp, R. J., Smith, J. F., and Parker, M. D. (2002). Recombinant chimeric western and eastern equine encephalitis viruses as potential vaccine candidates. Virology 302, 299–309. doi: 10.1006/viro.2002.1677

PubMed Abstract | Crossref Full Text | Google Scholar

Schoneboom, B. A., Lee, J. S., and Grieder, F. B. (2000). Early expression of IFN-alpha/beta and iNOS in the brains of Venezuelan equine encephalitis virus-infected mice. J. Interferon Cytokine Res. 20, 205–215. doi: 10.1089/107999000312621

PubMed Abstract | Crossref Full Text | Google Scholar

Schwarzbold, M. L., Rial, D., De Bem, T., Machado, D. G., Cunha, M. P., dos Santos, A. A., et al. (2010). Effects of traumatic brain injury of different severities on emotional, cognitive, and oxidative stress-related parameters in mice. J. Neurotrauma 27, 1883–1893. doi: 10.1089/neu.2010.1318

PubMed Abstract | Crossref Full Text | Google Scholar

Segal, B. M. (2007). The role of natural killer cells in curbing neuroinflammation. J. Neuroimmunol. 191, 2–7. doi: 10.1016/j.jneuroim.2007.09.006

PubMed Abstract | Crossref Full Text | Google Scholar

Sekijima, Y., Morita, H., and Yanagisawa, N. (1997). Follow-up of sarin poisoning in Matsumoto. Ann. Intern. Med. 127:1042. doi: 10.7326/0003-4819-127-11-199712010-00028

PubMed Abstract | Crossref Full Text | Google Scholar

Sell, S. L., Boone, D. R., Weisz, H. A., Cardenas, C., Willey, H. E., Bolding, I. J., et al. (2019). MicroRNA profiling identifies a novel compound with antidepressant properties. PLoS ONE 14:e0221163. doi: 10.1371/journal.pone.0221163

PubMed Abstract | Crossref Full Text | Google Scholar

Sellstrom, A., Algers, G., and Karlsson, B. (1985). Soman intoxication and the blood-brain barrier. Fundam. Appl. Toxicol. 5, S122–6. doi: 10.1016/0272-0590(85)90121-6

Crossref Full Text | Google Scholar

Sewell, D. L. (1995). Laboratory-associated infections and biosafety. Clin. Microbiol. Rev. 8, 389–405. doi: 10.1128/CMR.8.3.389

PubMed Abstract | Crossref Full Text | Google Scholar

Sharma, A., Bhattacharya, B., Puri, R. K., and Maheshwari, R. K. (2008). Venezuelan equine encephalitis virus infection causes modulation of inflammatory and immune response genes in mouse brain. BMC Genomics. 9:289. doi: 10.1186/1471-2164-9-289

PubMed Abstract | Crossref Full Text | Google Scholar

Sharma, A., and Knollmann-Ritschel, B. (2019). Current understanding of the molecular basis of venezuelan equine encephalitis virus pathogenesis and vaccine development. Viruses 11:E164. doi: 10.3390/v11020164

PubMed Abstract | Crossref Full Text | Google Scholar

Shechter, S., Thomas, D. R., Lundberg, L., Pinkham, C., Lin, S. C., Wagstaff, K. M., et al. (2017). Novel inhibitors targeting Venezuelan equine encephalitis virus capsid protein identified using in silico structure-based-drug-design. Sci. Rep. 7:17705. doi: 10.1038/s41598-017-17672-9

PubMed Abstract | Crossref Full Text | Google Scholar

Shi, J., Liu, D., Jin, Q., Chen, X., Zhang, R., Shi, T., et al. (2023). Whole-transcriptome analysis of repeated low-level sarin-exposed rat hippocampus and identification of cerna networks to investigate the mechanism of sarin-induced cognitive impairment. Biology 12:627. doi: 10.3390/biology12040627

PubMed Abstract | Crossref Full Text | Google Scholar

Shih, T. M., Duniho, S. M., and McDonough, J. H. (2003). Control of nerve agent-induced seizures is critical for neuroprotection and survival. Toxicol. Appl. Pharmacol. 188, 69–80. doi: 10.1016/S0041-008X(03)00019-X

PubMed Abstract | Crossref Full Text | Google Scholar

Shih, T. M., and McDonough, J. H. (2000). Efficacy of biperiden and atropine as anticonvulsant treatment for organophosphorus nerve agent intoxication. Arch. Toxicol. 74, 165–172. doi: 10.1007/s002040050670

PubMed Abstract | Crossref Full Text | Google Scholar

Shih, T. M., and McDonough, J. H. Jr. (1997). Neurochemical mechanisms in soman-induced seizures. J. Appl. Toxicol. 17, 255–564. doi: 10.1002/(SICI)1099-1263(199707)17:4&lt;255::AID-JAT441&gt;3.0.CO;2-D

Crossref Full Text | Google Scholar

Shih, T. M., Penetar, D. M., McDonough, J. H. Jr., Romano, J. A., and King, J. M. (1990). Age-related differences in soman toxicity and in blood and brain regional cholinesterase activity. Brain Res. Bull. 24, 429–436. doi: 10.1016/0361-9230(90)90097-J

PubMed Abstract | Crossref Full Text | Google Scholar

Shim, Y., Kim, J., Kim, H. S., Oh, J., Lee, S., Ha, E. J., et al. (2023). Intracranial pressure monitoring for acute brain injured patients: when, how, what should we monitor. Korean J. Neurotrauma 19, 149–161. doi: 10.13004/kjnt.2023.19.e32

PubMed Abstract | Crossref Full Text | Google Scholar

Shishido, H., Ueno, M., Sato, K., Matsumura, M., Toyota, Y., Kirino, Y., et al. (2019). Traumatic brain injury by weight-drop method causes transient amyloid-beta deposition and acute cognitive deficits in mice. Behav. Neurol. 2019:3248519. doi: 10.1155/2019/3248519

PubMed Abstract | Crossref Full Text | Google Scholar

Shively, S. B., Horkayne-Szakaly, I., Jones, R. V., Kelly, J. P., Armstrong, R. C., Perl, D. P., et al. (2016). Characterisation of interface astroglial scarring in the human brain after blast exposure: a post-mortem case series. Lancet Neurol. 15, 944–953. doi: 10.1016/S1474-4422(16)30057-6

PubMed Abstract | Crossref Full Text | Google Scholar

Sidell, F. R. (1974). Soman and sarin: clinical manifestations and treatment of accident of accidental poisoning by organophosphates. Clin. Toxicol. 7, 1–17. doi: 10.3109/15563657408987971

PubMed Abstract | Crossref Full Text | Google Scholar

Simon, L. V., Coffey, R., and Fischer, M. A. (2023). Western Equine Encephalitis. Treasure Island, FL: StatPearls.

Google Scholar

Singh, J., Kour, K., and Jayaram, M. B. (2012). Acetylcholinesterase inhibitors for schizophrenia. Cochrane Database Syst. Rev. 1:CD007967. doi: 10.1002/14651858.CD007967.pub2

PubMed Abstract | Crossref Full Text | Google Scholar

Sirkka, U., Nieminen, S. A., and Ylitalo, P. (1990). Neurobehavioral toxicity with low doses of sarin and soman. Methods Find. Exp. Clin. Pharmacol. 12, 245–250.

PubMed Abstract | Google Scholar

Skidmore, A. M., Adcock, R. S., Jonsson, C. B., Golden, J. E., and Chung, D. H. (2020). Benzamidine ML336 inhibits plus and minus strand RNA synthesis of Venezuelan equine encephalitis virus without affecting host RNA production. Antiviral Res. 174:104674. doi: 10.1016/j.antiviral.2019.104674

PubMed Abstract | Crossref Full Text | Google Scholar

Skidmore, A. M., and Bradfute, S. B. (2023). The life cycle of the alphaviruses: from an antiviral perspective. Antiviral Res. 209:105476. doi: 10.1016/j.antiviral.2022.105476

PubMed Abstract | Crossref Full Text | Google Scholar

Smith, D. H., Soares, H. D., Pierce, J. S., Perlman, K. G., Saatman, K. E., Meaney, D. F., et al. (1995). A model of parasagittal controlled cortical impact in the mouse: cognitive and histopathologic effects. J. Neurotrauma 12, 169–178. doi: 10.1089/neu.1995.12.169

PubMed Abstract | Crossref Full Text | Google Scholar

Smith, D. R., Schmaljohn, C. S., Badger, C., Ostrowski, K., Zeng, X., Grimes, S. D., et al. (2020). Comparative pathology study of Venezuelan, eastern, and western equine encephalitis viruses in non-human primates. Antiviral Res. 182:104875. doi: 10.1016/j.antiviral.2020.104875

PubMed Abstract | Crossref Full Text | Google Scholar

Soldin, O. P., and Mattison, D. R. (2009). Sex differences in pharmacokinetics and pharmacodynamics. Clin. Pharmacokinet. 48, 143–157. doi: 10.2165/00003088-200948030-00001

PubMed Abstract | Crossref Full Text | Google Scholar

Song, H., Konan, L. M., Cui, J., Johnson, C. E., Langenderfer, M., Grant, D., et al. (2018). Ultrastructural brain abnormalities and associated behavioral changes in mice after low-intensity blast exposure. Behav. Brain Res. 347, 148–157. doi: 10.1016/j.bbr.2018.03.007

PubMed Abstract | Crossref Full Text | Google Scholar

Song, J. (2023). Amygdala activity and amygdala-hippocampus connectivity: metabolic diseases, dementia, and neuropsychiatric issues. Biomed. Pharmacother. 162:114647. doi: 10.1016/j.biopha.2023.114647

PubMed Abstract | Crossref Full Text | Google Scholar

Song, Q., Xie, D., Pan, S., and Xu, W. (2015). Rapamycin protects neurons from brain contusion-induced inflammatory reaction via modulation of microglial activation. Mol. Med. Rep. 12, 7203–7210. doi: 10.3892/mmr.2015.4407

PubMed Abstract | Crossref Full Text | Google Scholar

Steele, K. E., Davis, K. J., Stephan, K., Kell, W., Vogel, P., Hart, M. K., et al. (1998). Comparative neurovirulence and tissue tropism of wild-type and attenuated strains of Venezuelan equine encephalitis virus administered by aerosol in C3H/HeN and BALB/c mice. Vet. Pathol. 35, 386–397. doi: 10.1177/030098589803500508

PubMed Abstract | Crossref Full Text | Google Scholar

Steele, K. E., and Twenhafel, N. A. (2010). REVIEW PAPER: pathology of animal models of alphavirus encephalitis. Vet. Pathol. 47, 790–805. doi: 10.1177/0300985810372508

PubMed Abstract | Crossref Full Text | Google Scholar

Stelfa, G., Svalbe, B., Vavers, E., Duritis, I., Dambrova, M., Zvejniece, L., et al. (2022). Moderate traumatic brain injury triggers long-term risks for the development of peripheral pain sensitivity and depressive-like behavior in mice. Front. Neurol. 13:985895. doi: 10.3389/fneur.2022.985895

PubMed Abstract | Crossref Full Text | Google Scholar

Stigler, L., Kohler, A., Koller, M., Job, L., Escher, B., Potschka, H., et al. (2022). Post-VX exposure treatment of rats with engineered phosphotriesterases. Arch. Toxicol. 96, 571–583. doi: 10.1007/s00204-021-03199-6

PubMed Abstract | Crossref Full Text | Google Scholar

Svensson, I., Waara, L., Johansson, L., Bucht, A., and Cassel, G. (2001). Soman-induced interleukin-1 beta mRNA and protein in rat brain. Neurotoxicology 22, 355–362. doi: 10.1016/S0161-813X(01)00022-5

PubMed Abstract | Crossref Full Text | Google Scholar

Taylor, K., Kolokoltsova, O., Patterson, M., Poussard, A., Smith, J., Estes, D. M., et al. (2012). Natural killer cell mediated pathogenesis determines outcome of central nervous system infection with Venezuelan equine encephalitis virus in C3H/HeN mice. Vaccine 30, 4095–4105. doi: 10.1016/j.vaccine.2012.03.076

PubMed Abstract | Crossref Full Text | Google Scholar

Taylor, K., Kolokoltsova, O., Ronca, S. E., Estes, M., and Paessler, S. (2017). Live, attenuated venezuelan equine encephalitis virus vaccine (TC83) causes persistent brain infection in mice with non-functional alphabeta T-cells. Front. Microbiol. 8:81. doi: 10.3389/fmicb.2017.00081

PubMed Abstract | Crossref Full Text | Google Scholar

Taylor, K. G., and Paessler, S. (2013). Pathogenesis of Venezuelan equine encephalitis. Vet. Microbiol. 167, 145–150. doi: 10.1016/j.vetmic.2013.07.012

PubMed Abstract | Crossref Full Text | Google Scholar

Teleanu, R. I., Chircov, C., Grumezescu, A. M., Volceanov, A., and Teleanu, D. M. (2019). Antioxidant therapies for neuroprotection-a review. J. Clin. Med. 8. doi: 10.3390/jcm8101659

PubMed Abstract | Crossref Full Text | Google Scholar

Testylier, G., Tonduli, L., and Lallement, G. (1999). Implication of gamma band in soman-induced seizures. Acta Biotheor. 47, 191–197. doi: 10.1023/A:1002626318172

PubMed Abstract | Crossref Full Text | Google Scholar

Thiermann, H., Szinicz, L., Eyer, F., Worek, F., Eyer, P., Felgenhauer, N., et al. (1999). Modern strategies in therapy of organophosphate poisoning. Toxicol. Lett. 107, 233–239. doi: 10.1016/S0378-4274(99)00052-1

PubMed Abstract | Crossref Full Text | Google Scholar

Thomas, D. R., Lundberg, L., Pinkham, C., Shechter, S., DeBono, A., Baell, J., et al. (2018). Identification of novel antivirals inhibiting recognition of Venezuelan equine encephalitis virus capsid protein by the importin alpha/beta1 heterodimer through high-throughput screening. Antiviral Res. 151, 8–19. doi: 10.1016/j.antiviral.2018.01.007

PubMed Abstract | Crossref Full Text | Google Scholar

Thompson, H. J., Pinto-Martin, J., and Bullock, M. R. (2003). Neurogenic fever after traumatic brain injury: an epidemiological study. J. Neurol. Neurosurg. Psychiatr. 74, 614–619. doi: 10.1136/jnnp.74.5.614

PubMed Abstract | Crossref Full Text | Google Scholar

Thorne, R. G., Hanson, L. R., Ross, T. M., Tung, D., and Frey, W. H. 2nd. (2008). Delivery of interferon-beta to the monkey nervous system following intranasal administration. Neuroscience 152, 785–797. doi: 10.1016/j.neuroscience.2008.01.013

PubMed Abstract | Crossref Full Text | Google Scholar

Tigertt, W. D., Crosby, W. H., Berge, T. O., Howie, D. L., Kress, S., Dangerfield, H. G., et al. (1962). The virus of Venezuelan equine encephalomyelitis as an antineoplastic agent in man. Cancer 15, 628–632. doi: 10.1002/1097-0142(196205/06)15:3&lt;628::AID-CNCR2820150326&gt;3.0.CO;2-A

PubMed Abstract | Crossref Full Text | Google Scholar

Tomkins, O., Feintuch, A., Benifla, M., Cohen, A., Friedman, A., Shelef, I., et al. (2011). Blood-brain barrier breakdown following traumatic brain injury: a possible role in posttraumatic epilepsy. Cardiovasc. Psychiatry Neurol. 2011:765923. doi: 10.1155/2011/765923

PubMed Abstract | Crossref Full Text | Google Scholar

Tressler, C. M., Wadsworth, B., Carriero, S., Dillman, N., Crawford, R., Hahm, T. H., et al. (2024). Characterization of humanized mouse model of organophosphate poisoning and detection of countermeasures via MALDI-MSI. Int. J. Mol. Sci. 25:5624. doi: 10.3390/ijms25115624

PubMed Abstract | Crossref Full Text | Google Scholar

Tu, A. T. (2020). The use of VX as a terrorist agent: action by Aum Shinrikyo of Japan and the death of Kim Jong-Nam in Malaysia: four case studies. Glob. Secur. Health Sci. Policy 5, 48–56. doi: 10.1080/23779497.2020.1801352

Crossref Full Text | Google Scholar

Tucker, L. B., Burke, J. F., Fu, A. H., and McCabe, J. T. (2017). Neuropsychiatric symptom modeling in male and female C57BL/6J Mice after experimental traumatic brain injury. J. Neurotrauma 34, 890–905. doi: 10.1089/neu.2016.4508

PubMed Abstract | Crossref Full Text | Google Scholar

Tucker, L. B., and McCabe, J. T. (2021). Measuring anxiety-like behaviors in rodent models of traumatic brain injury. Front. Behav. Neurosci. 15:682935. doi: 10.3389/fnbeh.2021.682935

PubMed Abstract | Crossref Full Text | Google Scholar

Uyanikgil, Y., Cavusoglu, T., Kilic, K. D., Yigitturk, G., Celik, S., Tubbs, R. S., et al. (2017). Useful effects of melatonin in peripheral nerve injury and development of the nervous system. J. Brachial Plex. Peripher. Nerve Inj. 12, e1–e6. doi: 10.1055/s-0036-1597838

PubMed Abstract | Crossref Full Text | Google Scholar

van der Staay, F. J., Arndt, S. S., and Nordquist, R. E. (2009). Evaluation of animal models of neurobehavioral disorders. Behav. Brain Funct. 5:11. doi: 10.1186/1744-9081-5-11

PubMed Abstract | Crossref Full Text | Google Scholar

Van Helden, H. P. (2002). Low level exposure to gb vapor in air: diagnosis/dosimetry, lowest observable effect levels, performance-incapacitation, and possible delayed effects final addendum. Fort Detrick, MD: US Army Medical Research and Materiel Command. 21702-5012.

Google Scholar

van Helden, H. P., Joosen, M. J., and Philippens, I. H. (2011). Non-enzymatic pretreatment of nerve agent (soman) poisoning: a brief state-of-the-art review. Toxicol. Lett. 206, 35–40. doi: 10.1016/j.toxlet.2011.04.021

PubMed Abstract | Crossref Full Text | Google Scholar

Vella, M. A., Crandall, M. L., and Patel, M. B. (2017). Acute management of traumatic brain injury. Surg. Clin. North Am. 97, 1015–1030. doi: 10.1016/j.suc.2017.06.003

PubMed Abstract | Crossref Full Text | Google Scholar

Verduzco-Mendoza, A., Mota-Rojas, D., Olmos Hernandez, S. A., Galvez-Rosas, A., Aguirre-Perez, A., Cortes-Altamirano, J. L., et al. (2023). Traumatic brain injury extending to the striatum alters autonomic thermoregulation and hypothalamic monoamines in recovering rats. Front. Neurosci. 17:1304440. doi: 10.3389/fnins.2023.1304440

PubMed Abstract | Crossref Full Text | Google Scholar

Victor, J., Smith, D. G., and Pollack, A. D. (1956). The comparative pathology of Venezuelan equine encephalomyelitis. J. Infect. Dis. 98, 55–66. doi: 10.1093/infdis/98.1.55

PubMed Abstract | Crossref Full Text | Google Scholar

Vilcarromero, S., Aguilar, P. V., Halsey, E. S., Laguna-Torres, V. A., Razuri, H., Perez, J., et al. (2010). Venezuelan equine encephalitis and 2 human deaths, Peru. Emerging Infect. Dis. 16, 553–556. doi: 10.3201/eid1603.090970

PubMed Abstract | Crossref Full Text | Google Scholar

Villari, P., Spielman, A., Komar, N., McDowell, M., and Timperi, R. J. (1995). The economic burden imposed by a residual case of eastern encephalitis. Am. J. Trop. Med. Hyg. 52, 8–13. doi: 10.4269/ajtmh.1995.52.8

PubMed Abstract | Crossref Full Text | Google Scholar

Virmani, N., Bera, B. C., Gulati, B. R., Karuppusamy, S., Singh, B. K., Kumar Vaid, R., et al. (2010). Descriptive epidemiology of equine influenza in India (2008-2009): temporal and spatial trends. Vet. Ital. 46, 449–458.

PubMed Abstract | Google Scholar

Vogel, P., Kell, W. M., Fritz, D. L., Parker, M. D., and Schoepp, R. J. (2005). Early events in the pathogenesis of eastern equine encephalitis virus in mice. Am. J. Pathol. 166, 159–171. doi: 10.1016/S0002-9440(10)62241-9

PubMed Abstract | Crossref Full Text | Google Scholar

Vucinic, S., Antonijevic, B., Tsatsakis, A. M., Vassilopoulou, L., Docea, A. O., Nosyrev, A. E., et al. (2017). Environmental exposure to organophosphorus nerve agents. Environ. Toxicol. Pharmacol. 56, 163–171. doi: 10.1016/j.etap.2017.09.004

PubMed Abstract | Crossref Full Text | Google Scholar

Wang, H. Y., Cecon, E., Dam, J., Pei, Z., Jockers, R., Burns, L. H., et al. (2023). Simufilam reverses aberrant receptor interactions of filamin A in Alzheimer's disease. Int. J. Mol. Sci. 24. doi: 10.3390/ijms241813927

PubMed Abstract | Crossref Full Text | Google Scholar

Wang, J., Lu, X., Gao, R., Pei, C., and Wang, H. (2022). Current progress for retrospective identification of nerve agent biomarkers in biological samples after exposure. Toxics 10. doi: 10.3390/toxics10080439

PubMed Abstract | Crossref Full Text | Google Scholar

Weaver, S. C., Anishchenko, M., Bowen, R., Brault, A. C., Estrada-Franco, J. G., Fernandez, Z., et al. (2004a). Genetic determinants of Venezuelan equine encephalitis emergence. Arch. Virol. Suppl. 43–64. doi: 10.1007/978-3-7091-0572-6_5

PubMed Abstract | Crossref Full Text | Google Scholar

Weaver, S. C., Ferro, C., Barrera, R., Boshell, J., and Navarro, J. C. (2004b). Venezuelan equine encephalitis. Annu. Rev. Entomol. 49, 141–174. doi: 10.1146/annurev.ento.49.061802.123422

PubMed Abstract | Crossref Full Text | Google Scholar

Weaver, S. C., Winegar, R., Manger, I. D., and Forrester, N. L. (2012). Alphaviruses: population genetics and determinants of emergence. Antiviral Res. 94, 242–257. doi: 10.1016/j.antiviral.2012.04.002

PubMed Abstract | Crossref Full Text | Google Scholar

Webster, I. (2024). $992 in 1995→2024 | Inflation Calculator. Official Inflation Data, Alioth Finance. Available at: https://wwwofficialdataorg/us/inflation/1995?amount=992 (accessed November 1, 2024).

Google Scholar

Weil, Z. M., Karelina, K., Whitehead, B., Velazquez-Cruz, R., Oliverio, R., Pinti, M., et al. (2021). Mild traumatic brain injury increases vulnerability to cerebral ischemia in mice. Exp. Neurol. 342:113765. doi: 10.1016/j.expneurol.2021.113765

PubMed Abstract | Crossref Full Text | Google Scholar

Wesseling, C., Keifer, M., Ahlbom, A., McConnell, R., Moon, J. D., Rosenstock, L., et al. (2002). Long-term neurobehavioral effects of mild poisonings with organophosphate and n-methyl carbamate pesticides among banana workers. Int. J. Occup. Environ. Health 8, 27–34. doi: 10.1179/oeh.2002.8.1.27

PubMed Abstract | Crossref Full Text | Google Scholar

Wiener, S. W., and Hoffman, R. S. (2004). Nerve agents: a comprehensive review. J. Intensive Care Med. 19, 22–37. doi: 10.1177/0885066603258659

PubMed Abstract | Crossref Full Text | Google Scholar

Wiercinski, A., and Jackson, J. P. (2024). Nerve Agents. Treasure Island, FL: StatPearls.

Google Scholar

Williams, E. P., Xue, Y., Lee, J., Fitzpatrick, E. A., Kong, Y., Reichard, W., et al. (2023). Deep spatial profiling of Venezuelan equine encephalitis virus reveals increased genetic diversity amidst neuroinflammation and cell death during brain infection. J. Virol. 97:e0082723. doi: 10.1128/jvi.00827-23

PubMed Abstract | Crossref Full Text | Google Scholar

Williams, J. A., Long, S. Y., Zeng, X., Kuehl, K., Babka, A. M., Davis, N. M., et al. (2022). Eastern equine encephalitis virus rapidly infects and disseminates in the brain and spinal cord of cynomolgus macaques following aerosol challenge. PLoS Negl. Trop. Dis. 16:e0010081. doi: 10.1371/journal.pntd.0010081

PubMed Abstract | Crossref Full Text | Google Scholar

Wishart, D. S., Feunang, Y. D., Guo, A. C., Lo, E. J., Marcu, A., Grant, J. R., et al. (2018). DrugBank 5.0: a major update to the DrugBank database for 2018. Nucleic Acids Res. 46, D1074–D1082. doi: 10.1093/nar/gkx1037

PubMed Abstract | Crossref Full Text | Google Scholar

Wishart, D. S., Knox, C., Guo, A. C., Shrivastava, S., Hassanali, M., Stothard, P., et al. (2006). DrugBank: a comprehensive resource for in silico drug discovery and exploration. Nucleic Acids Res. 34(Database issue), D668–D672. doi: 10.1093/nar/gkj067

PubMed Abstract | Crossref Full Text | Google Scholar

Woodard, C. L., Calamaio, C. A., Kaminskis, A., Anderson, D. R., Harris, L. W., Martin, D. G., et al. (1994). Erythrocyte and plasma cholinesterase activity in male and female rhesus monkeys before and after exposure to sarin. Fundam. Appl. Toxicol. 23, 342–347. doi: 10.1006/faat.1994.1114

PubMed Abstract | Crossref Full Text | Google Scholar

World Health Organization (2023). Western equine encephalitis in Argentina 2023. Available at: https://www.who.int/emergencies/disease-outbreak-news/item/2023-DON499 (accessed December 28, 2023).

Google Scholar

Wu, J., and Lipinski, M. M. (2019). Autophagy in neurotrauma: good, bad, or dysregulated. Cells 8. doi: 10.3390/cells8070693

PubMed Abstract | Crossref Full Text | Google Scholar

Wu, Y., Wu, H., Guo, X., Pluimer, B., and Zhao, Z. (2020). Blood-brain barrier dysfunction in mild traumatic brain injury: evidence from preclinical murine models. Front. Physiol. 11:1030. doi: 10.3389/fphys.2020.01030

PubMed Abstract | Crossref Full Text | Google Scholar

Wyck, D. W. V., Grant, G. A., and Laskowitz, D. T. (2015). Penetrating traumatic brain injury: A review of current evaluation and management concepts. J. Neurol. Neurophysiol. 6, 1–7.

Google Scholar

Xiong, Y., Mahmood, A., and Chopp, M. (2013). Animal models of traumatic brain injury. Nat. Rev. Neurosci. 14, 128–142. doi: 10.1038/nrn3407

PubMed Abstract | Crossref Full Text | Google Scholar

Xu, S. Y., Liu, M., Gao, Y., Cao, Y., Bao, J. G., Lin, Y. Y., et al. (2019). Acute histopathological responses and long-term behavioral outcomes in mice with graded controlled cortical impact injury. Neural Regen Res. 14, 997–1003. doi: 10.4103/1673-5374.250579

PubMed Abstract | Crossref Full Text | Google Scholar

Xu, X., Cowan, M., Beraldo, F., Schranz, A., McCunn, P., Geremia, N., et al. (2021). Repetitive mild traumatic brain injury in mice triggers a slowly developing cascade of long-term and persistent behavioral deficits and pathological changes. Acta Neuropathol. Commun. 9:60. doi: 10.1186/s40478-021-01161-2

PubMed Abstract | Crossref Full Text | Google Scholar

Yang, S. H., Gustafson, J., Gangidine, M., Stepien, D., Schuster, R., Pritts, T. A., et al. (2013). A murine model of mild traumatic brain injury exhibiting cognitive and motor deficits. J. Surg. Res. 184, 981–8. doi: 10.1016/j.jss.2013.03.075

PubMed Abstract | Crossref Full Text | Google Scholar

Yi, J. H., and Hazell, A. S. (2006). Excitotoxic mechanisms and the role of astrocytic glutamate transporters in traumatic brain injury. Neurochem. Int. 48, 394–403. doi: 10.1016/j.neuint.2005.12.001

PubMed Abstract | Crossref Full Text | Google Scholar

Yu, S., Kaneko, Y., Bae, E., Stahl, C. E., Wang, Y., van Loveren, H., et al. (2009). Severity of controlled cortical impact traumatic brain injury in rats and mice dictates degree of behavioral deficits. Brain Res. 1287, 157–163. doi: 10.1016/j.brainres.2009.06.067

PubMed Abstract | Crossref Full Text | Google Scholar

Zacks, M. A., and Paessler, S. (2010). Encephalitic alphaviruses. Vet. Microbiol. 140, 281–286. doi: 10.1016/j.vetmic.2009.08.023

PubMed Abstract | Crossref Full Text | Google Scholar

Zhao, H., Zhou, M., Liu, Y., Jiang, J., and Wang, Y. (2023). Recent advances in anxiety disorders: focus on animal models and pathological mechanisms. Animal Model Exp. Med. 6, 559–572. doi: 10.1002/ame2.12360

PubMed Abstract | Crossref Full Text | Google Scholar

Zheng, R. Z., Lee, K. Y., Qi, Z. X., Wang, Z., Xu, Z. Y., Wu, X. H., et al. (2022). Neuroinflammation following traumatic brain injury: take it seriously or not. Front. Immunol. 13:855701. doi: 10.3389/fimmu.2022.855701

PubMed Abstract | Crossref Full Text | Google Scholar

Zimmer, L. A., Ennis, M., and Shipley, M. T. (1997). Soman-induced seizures rapidly activate astrocytes and microglia in discrete brain regions. J. Comp. Neurol. 378, 482–492. doi: 10.1002/(SICI)1096-9861(19970224)378:4&lt;482::AID-CNE4&gt;3.0.CO;2-Z

PubMed Abstract | Crossref Full Text | Google Scholar

Zlotnik, I., Peacock, S., Grant, D. P., and Batter-Hatton, D. (1972). The pathogenesis of western equine encephalitis virus (W.E.E.) in adult hamsters with special reference to the long and short term effects on the C.N.S. of the attenuated clone 15 variant. Br. J. Exp. Pathol. 53, 59–77.

PubMed Abstract | Google Scholar