This article is a commentary on:
Organ Cultures for Retinal Diseases
Stephen R. Mut
Maribel Vazquez- Department of Biomedical Engineering, Rutgers, The State University of New Jersey, New Brunswick, NJ, United States
A Commentary on
Organ Cultures for Retinal Diseases
by Hurst, J., Fietz, A., Tsai, T., Joachim, S. C., and Schnichels, S. (2020). Front. Neurosci. 14:583392. doi: 10.3389/fnins.2020.583392
The review by Hurst et al. (2020) is a comprehensive article published within this journal about the use of organotypic culture systems as models to study retinal diseases. The article noted that use of microfluidic technologies, such as microelectrode arrays (MEAs), can be significant in measuring cellular activity within organ culture systems (Hurst et al., 2020). An additional emerging area for microfluidics is their integration with explants to enrich transplantation strategies used to treat retinal degenerative diseases.
Progressive vision loss in adults is escalating worldwide, as the incidence of macular degeneration and diabetic retinopathy are expected to exceed 300 million and 642 million, respectively, by 2040 (Mitchell et al., 2018; Simo-Servat et al., 2019). The retina consists of a varied network of neurons that synapse with one another across three nuclear layers. Damage to any one type of neuron within this intricate network propagates dysfunction to result in progressive vision loss.
Contemporary cell replacement therapies offer exciting promise to restore vision by replacing damaged neurons with transplanted stem cells. Numerous platforms have been developed to elucidate the cellular mechanisms able to promote stem cell integration within mature retinal hosts (Wu et al., 2018). However, ongoing projects have produced mixed results, including low stem cell survival and the inability of stem cells to differentiate and/or position themselves appropriately within the retinal network (Gokoffski et al., 2019). A variety of in vitro and organotypic platforms have been developed to examine native stem cell behaviors within microscale environments (reviewed in Greene et al., 2019). Surprisingly, few of these projects have incorporated microfluidic technologies to model cues from damaged adult retina, such as fields of injury cytokines and degraded cellular matrixes (reviewed in Vazquez, 2020), that differ significantly from stem cell environments. A current thrust is to bridge microfluidic technologies with explanted retina to develop hybrid, quantitative models to examine stem cell behaviors within adult, organotypic cultures.
Initial hybrid models (Figure 1) cultured retinal explants within micro-scale transwell systems to measure long-term viability (Rettinger and Wang, 2018), while newer models integrated microfluidic perfusion systems for controlled delivery of neurotransmitters and therapeutics (Dodson et al., 2015; Rountree et al., 2017). Most recently, our group developed a hybrid system called the Ex Vivo Eye Facsimile System (EVES) to examine how extrinsic factors, such as chemical and electrical gradients, can promote appropriate stem cell positioning within retinal hosts (Mishra et al., 2019; Vazquez et al., 2020). Our system consists of a 3D environment that can be rapidly prototyped to meet the geometric constraints of enucleated eyes derived from a variety of animal models. Our preliminary EVES studies illustrated that combined electrochemical fields increased the numbers of motile stem cells and the distances migrated within rodent eye facsimiles. The integration of microfluidics with organotypic retinal cultures will therefore produce a new generation of quantitative platforms that enable newfound applications of external fields to enrich stem cell replacement strategies.
FIGURE 1
Figure 1. Current hybrid explant systems for studying the retina. (A) An ex vivo chamber for a porcine retina explant capable of maintaining tissue viability for 1–2 weeks to enable long-term investigation of the ex vivo retina (Rettinger and Wang, 2018). (B) A glutamate perfusion system to stimulate retinal neurons with neurotransmitters to augment the phototransduction process that occurs within the body (Rountree et al., 2017). (C) Retina on a chip microfluidic perfusion assay system for point access delivery of therapeutics to specific parts of the rat retina (Dodson et al., 2015). (D) Ex Vivo Eye Facsimile (EVES) hybrid explant system designed for delivery of electrical and chemical stimulation to a whole-enucleated eye explant from mouse (Vazquez et al., 2020).
Author Contributions
SM developed and wrote the manuscript. MV wrote and provided edits to the manuscript. All authors approved the final manuscript version for publication.
Funding
This work was supported by the US National Science Foundation (NSF CBET 180441) and the US National Institutes of Health (NEI 1R21 EY031439-01).
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher's Note
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References
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Vazquez, M., Pena, J. S., and Mut, S. (2020). An Ex Vivo Eye Facsimile System, (EVES) to evaluate transplantation strategies for cell replacement therapy. Investig. Ophthalmol. Vis. Sci. 61:795. Available online at: https://iovs.arvojournals.org/article.aspx?articleid=2769637
Keywords: microfluidics, vision loss, in vitro, stem cells, regeneration
Citation: Mut SR and Vazquez M (2021) Commentary: Organ Cultures for Retinal Diseases. Front. Neurosci. 15:714094. doi: 10.3389/fnins.2021.714094
Received: 24 May 2021; Accepted: 15 June 2021;
Published: 23 July 2021.
Edited by:
Miguel Flores-Bellver, University of Colorado Anschutz Medical Campus, United StatesReviewed by:
Natalia Martínez-Gil, University of Alicante, SpainMaría Miranda, Universidad CEU Cardenal Herrera, Spain
Copyright © 2021 Mut and Vazquez. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Maribel Vazquez, bWFyaWJlbC52YXpxdWV6JiN4MDAwNDA7cnV0Z2Vycy5lZHU=
†ORCID: Stephen R. Mut orcid.org/0000-0002-1535-8342