This article is a correction to:
A Simplified In vitro Experimental Model Encompasses the Essential Features of Sleep
Ilaria Colombi
Federico Tinarelli
Valentina Pasquale
Valter Tucci
Michela Chiappalone- 1Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Genova, Italy
- 2Dipartimento di Neuroscienze, Riabilitazione, Oftalmologia, Genetica e Scienze Materno-Infantili (DINOGMI), Università degli Studi di Genova, Genova, Italy
A corrigendum on
A Simplified In vitro Experimental Model Encompasses the Essential Features of Sleep
by Colombi, I., Tinarelli, F., Pasquale, V., Tucci, V., and Chiappalone, M. (2016). Front. Neurosci. 10:315. doi: 10.3389/fnins.2016.00315
Reason for Corrigendum:
The primer pairs for the gene Homer1 reported in the original manuscript referred to the longer isoforms Homer1b/c and not Homer1a. Moreover, we added some missing references. Therefore
at Page 2, Third paragraph, 11th line: the reference (Tateno et al., 2005; Corner, 2013) is corrected with (Tateno et al., 2005; Kaufman et al., 2012; Corner, 2013). The authors apologize for the missing reference. This error does not change the main scientific conclusions of the article;
at Page 5, Table 1: Homer1a is corrected with Homer1b/c. The authors apologize for the mistake. This error does not change the main scientific conclusions of the article;
at Page 9, Third paragraph, 11th line: Homer1a is corrected with Homer1b/c. The authors apologize for the mistake. This error does not change the main scientific conclusions of the article;
at Page 9, Third paragraph, 16th line: the phrase “In addition to the classical markers of the circadian and the homeostatic control of sleep” is corrected with “In addition to the classical markers of the circadian and the synaptic homeostasis control of sleep.” The authors apologize for the mistake. This error does not change the main scientific conclusions of the article;
at Page 9, Second paragraph, 1st line: the phrase “As a complement of the above conclusions, gene expression profile in our study confirmed an opposite effect on the circadian and the homeostatic components of sleep-like states” is corrected with “As a complement of the above conclusions, gene expression profile in our study confirmed an opposite effect on the circadian and the synaptic homeostatic components.” The authors apologize for the mistake. This error does not change the main scientific conclusions of the article;
at Page 9, Second paragraph, 10th line: the phrase “However, Homer1a, a gene widely considered as the main molecular marker of the homeostatic control of sleep (Hinard et al., 2012), was not affected by the treatment” is corrected with “However, Homer1b/c, a gene considered as a molecular marker of the synaptic excitability response to stimulation (Ango et al., 2000; Nakano-Kobayashi et al., 2014; Cao et al., 2015), was not affected by the treatment.” The authors apologize for the mistake. This error does not change the main scientific conclusions of the article;
at Page 9, Second paragraph, 17th line: the phrase “can we use in vitro experimental model to dissect molecular markers of homeostatic and circadian control of sleep? Indeed, PER2 is a marker of the circadian control of sleep (Kopp et al., 2002; Shiromani et al., 2004) while Homer1a is an important marker of the homeostatic process of sleep” is corrected with the phrase “can we use in vitro experimental model to dissect molecular markers of synaptic homeostasis and circadian control of sleep? Indeed, PER2 is a marker of the circadian control of sleep (Kopp et al., 2002; Shiromani et al., 2004) while Homer1b/c is an important marker of neuronal excitatory synapses activity.” The authors apologize for the mistake. This error does not change the main scientific conclusions of the article.
Author Contributions
Original Research article: IC, MC and VT designed the work. IC performed the electrophysiology experiments. FT performed the gene expressions. IC, FT, VP, and MC analyzed the data. IC, FT, VT, and MC wrote the manuscript. IC, VP, VT, and MC revised the manuscript. MC and VT supervised the study and equally contributed. Corrigendum: FT and VT wrote the corrigendum. MC, IC, and VP revised the corrigendum. All authors approved the final version.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
References
Ango, F., Pin, J. P., Tu, J. C., Xiao, B., Worley, P. F., Bockaert, J., et al. (2000). Dendritic and axonal targeting of type 5 metabotropic glutamate receptor is regulated by Homer1 proteins and neuronal excitation. J. Neurosci. 20, 8710–9716.
Cao, L., Tian, Y., Jiang, Y., Zhang, G. J., Lei, H., and Di, Z. l. (2015). Down-regulation of Homer1b/c protects against chemically induced seizures through inhibition of mTOR signaling. Cell Physiol. Biochem. 35, 1633–1642. doi: 10.1159/000373977
Corner, M. A. (2013). From neural plate to cortical arousal-a neuronal network theory of sleep derived from in vitro “model” systems for primordial patterns of spontaneous bioelectric activity in the vertebrate central nervous system. Brain Sci. 3, 800–820. doi: 10.3390/brainsci3020800
Hinard, V., Mikhail, C., Pradervand, S., Curie, T., Houtkooper, R. H., Auwerx, J., et al. (2012). Key electrophysiological, molecular, and metabolic signatures of sleep and wakefulness revealed in primary cortical cultures. J. Neurosci. 32, 12506–12517. doi: 10.1523/JNEUROSCI.2306-12.2012
Kaufman, M., Corner, M. A., and Ziv, N. E. (2012). Long-term relationships between cholinergic tone, synchronous bursting and synaptic remodeling. PLoS ONE 7:e40980. doi: 10.1371/journal.pone.0040980
Kopp, C., Albrecht, U., Zheng, B., and Tobler, I. (2002). Homeostatic sleep regulation is preserved in mPer1 and mPer2 mutant mice. Eur. J. Neurosci. 16, 1099–1106. doi: 10.1046/j.1460-9568.2002.02156.x
Nakano-Kobayashi, A., Tai, Y., Nadif Kasri, N., and Van Aelst, L. (2014). The X-linked mental retardation protein OPHN1 interacts with Homer1b/c to control spine endocytic zone positioning and expression of synaptic potentiation. J. Neurosci. 34, 8665–8671. doi: 10.1523/JNEUROSCI.0894-14.2014
Shiromani, P. J., Xu, M., Winston, E. M., Shiromani, S. N., Gerashchenko, D., and Weaver, D. R. (2004). Sleep rhythmicity and homeostasis in mice with targeted disruption of mPeriod genes. Am. J. Physiol. Regul. Integr. Comp. Physiol. 287, R47–R57. doi: 10.1152/ajpregu.00138.2004
Keywords: cortical culture, microelectrode arrays, homeostasis, spike train analysis, local field potentials, gene expression
Citation: Colombi I, Tinarelli F, Pasquale V, Tucci V and Chiappalone M (2016) Corrigendum: A Simplified In vitro Experimental Model Encompasses the Essential Features of Sleep. Front. Neurosci. 10:409. doi: 10.3389/fnins.2016.00409
Received: 28 July 2016; Accepted: 24 August 2016;
Published: 07 September 2016.
Edited and reviewed by: Michele Giugliano, University of Antwerp, Belgium
Copyright © 2016 Colombi, Tinarelli, Pasquale, Tucci and Chiappalone. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Michela Chiappalone, bWljaGVsYS5jaGlhcHBhbG9uZUBpaXQuaXQ=
†These authors have contributed equally to this work.