Abstract
During normal aging or neurodegenerative diseases, neuronal survival and function depend on protein homeostasis, which is regulated by multiple mechanisms, including the microRNA (miRNA) pathway. In different cells types, the absence of Dicer, a key miRNA processing enzyme, leads to neurodegeneration through cell-autonomous and non-cell-autonomous mechanisms. Loss of certain miRNAs also causes neurodegeneration in some model organisms. On the other hand, miRNA expression is misregulated in patients with different neurodegenerative diseases. Thus, the miRNA pathway appears to be essential in the pathogenesis of several age-dependent neurodegenerative conditions; however, our understanding of the underlying mechanism remains rudimentary. The precise causal relationships between specific miRNAs and neurodegeneration in humans need to be further investigated.
Introduction
Alzheimer’s disease (AD), frontotemporal dementia (FTD), Parkinson’s disease (PD), and other neurodegenerative disorders are a major health problem in both developed and developing countries (Hampel et al., 2011; Reitz et al., 2011; Wittchen et al., 2011). Since no effective treatments are available, it is unlikely that the adverse societal effects of these disorders will be substantially alleviated in the near future. These disorders are characterized by progressive neuronal dysfunction that initially affects selected groups of neurons in specialized neuronal circuits. A number of cellular and molecular mechanisms lead to neuronal demise. Among them, neurotoxicity induced by misfolding, mislocalization, or abnormally elevated concentrations of particular protein species seems to be a common theme (Jucker and Walker, 2011; Lee et al., 2012; Selkoe et al., 2012). Mutations in several genes that are apparently functionally unrelated can cause the same neurodegenerative disease. Multiple environmental factors (e.g., viral infections and exposure to certain toxins) might also contribute to the development of a neurodegenerative disease (Ahmed and Wicklund, 2011; Gao and Hong, 2011). Much remained to be learned about how the complex interactions of environmental and genetic factors initially lead to the misregulation of protein homeostasis and subsequently to neuronal dysfunction.
MicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate gene expression by degrading their target mRNAs or repressing their translation (Ambros et al., 2003; Bartel, 2004; Ghildiyal and Zamore, 2009). Initially discovered in Caenorhabditis elegans (Lee et al., 1993), miRNAs have been found in plants, invertebrates, mammals, and humans (Bartel, 2009). Two major features of miRNAs indicate their potential contributions to neurodegenerative diseases. First, miRNAs can simultaneously regulate many target transcripts, and up to 50% of all coding genes may be regulated by miRNAs (Krol et al., 2010). Thus, miRNAs are central regulators of genetic networks. Second, miRNAs ensure stable protein levels under variable conditions and are therefore essential for the robustness of biological processes (Herranz and Cohen, 2010). Given the importance of protein homeostasis and the diversity of cellular pathways potentially leading to neurodegeneration, it has been hypothesized that miRNAs might contribute to neurodegenerative diseases (Eacker et al., 2009; Lau and de Strooper, 2010; Sonntag, 2010).
In this review, we briefly describe the biogenesis of miRNAs and their potential involvement in the evolution of the human brain. Then we will discuss accumulating evidence that miRNAs are important contributors to neurodegenerative diseases. Indeed, some observations suggest that miRNA alterations can disrupt protein homeostasis and may be at the root of neurodegenerative processes (Figure 2). Conversely, other data strongly suggest that altered miRNA networks are a consequence of abnormal neuronal physiology (Figure 3). Examples in specific neurodegenerative diseases will be presented.
miRNA Biogenesis
Although several alternative mechanisms also exist (Yang and Lai, 2011), the canonical pathway for miRNA biogenesis involves a primary transcript generated by RNA polymerase II (Lee et al., 2004). The primary miRNA is cleaved by a nuclear complex containing Drosha and DGCR8, giving rise to a hairpin precursor molecule (pre-miRNA) of 70–100 nt (Lee et al., 2003; Han et al., 2004). This pre-miRNA is exported to the cytoplasm, where Dicer, a RNA-III nuclease, catalyzes the final cleavage in the maturation process, resulting in an imperfect RNA duplex (Hutvagner et al., 2001). One strand (guide strand) is loaded into an RNA-induced silencing complex (RISC) to bind the target mRNA; the other strand (passenger strand) is usually destroyed (Chendrimada et al., 2005; Bartel, 2009). miRNAs control gene expression at the post-transcriptional level through imperfect base pairing with specific sequences, located mostly in the 3′UTRs of mRNAs. After recognition, miRNA–target interactions often result in mRNA degradation or inhibition of mRNA translation (Krol et al., 2010; Figure 1).
Figure 1
Role of miRNA in Brain Evolution
Neurodegenerative diseases are considered devastating disorders because they often impair cognitive and executive functions. Understanding how the human brain acquired such functions is a challenging task that might also provide important insights into the mechanisms of neurodegeneration. It was noted four decades ago that genetic differences among species do not account for brain divergence and that brain evolution could have been driven by changes in gene expression levels (King and Wilson, 1975). Early comparative transcriptome analyses confirmed this hypothesis and revealed more human-specific than chimpanzee-specific expression changes in the prefrontal cortex (PFC); no such changes were observed in blood, liver, or other tissues (Enard et al., 2002; Caceres et al., 2003). These findings supported the idea that brain-specific changes in gene expression levels shaped the evolution of the nervous system.
To determine whether miRNAs participated in this process, miRNA expression profiles in human and chimpanzee brains were compared (Berezikov et al., 2006). Many of the newly identified brain miRNAs were expressed only in humans, and many were restricted to primates. Another study reported that 10–35% of miRNAs were expressed in the human brain but not in chimpanzee or macaque brains (Hu et al., 2011). More importantly, developmental profiles of brain miRNAs and their target genes showed the fastest rates of human-specific evolutionary change (Somel et al., 2011). Although experimental evidence is still lacking, it is an attractive hypothesis that rapidly evolving miRNAs in the human brain are essential for neuronal function and maintenance.
Global Loss of miRNAs Causes Neurodegeneration
Genetic disruption of miRNAs biogenesis pathways has been used to probe the potential link between miRNAs and neurodegeneration. In mouse cerebellar Purkinje cells, conditional knockout of Dicer leads to age-dependent cerebellar degeneration and ataxia (Schaefer et al., 2007). Cell-type specific deletion of Dicer in striatal, retinal, spinal, and cortical neurons produced similar results (Cuellar et al., 2008; Damiani et al., 2008; Davis et al., 2008; Haramati et al., 2010). Dicer deletion also altered the phosphorylation pattern of tau before neuronal cell loss (Hebert et al., 2010), indicating that some mechanisms of neurodegeneration might be controlled through miRNAs. Of note, neurodegeneration in the absence of Dicer could also result from the toxic accumulation of pre-miRNAs or from the loss of other Dicer functions unrelated to miRNAs biogenesis. However, reduced production of a small proportion of miRNAs as a result of DGCR8 haploinsufficiency also leads to neuronal dysfunction (Stark et al., 2008; Fenelon et al., 2011; Schofield et al., 2011), supporting the notion that neurodegeneration could indeed arise from loss of miRNAs.
Glial cell defects may also profoundly influence neuronal survival (Ilieva et al., 2009; Prinz et al., 2011). Indeed, neurodegeneration ensues after targeted deletion of Dicer in astrocytes (Tao et al., 2011), oligodendrocytes (Shin et al., 2009), and Schwann cells (Pereira et al., 2010; Verrier et al., 2010; Wu et al., 2012). Thus, alteration of miRNA networks has the potential to disrupt neuronal function in a cell-autonomous or non-cell-autonomous manner and lead to neurodegenerative phenotypes.
Loss of Individual miRNAs Leads to Neurodegeneration
Genetic analyses have revealed essential roles for specific miRNAs in long-term neuronal survival, for example in mice lacking miR-124-1 (Sanuki et al., 2011), one of the most well studied miRNAs in neuronal development (Gao, 2010). The mouse genome has three miR-124 loci (miR-124-1, -2, and -3). Deletion of miR-124-1, the dominant source of this miRNA, increases apoptosis in the hippocampus and retina, causing a significant decrease in brain size. Furthermore, in the tail-suspension test, adult mutant mice exhibit a front and hind limb clasping response, a common phenotype in mouse models of neurodegenerative disorders. This effect seems to be mediated by regulation of the transcription factor Lhx2 (Sanuki et al., 2011). It is unclear whether the neurodegeneration also reflects the absence of miR-124’s well-documented developmental functions (Cao et al., 2007; Visvanathan et al., 2007; De Pietri Tonelli et al., 2008; Cheng et al., 2009; Maiorano and Mallamaci, 2009). This question could be answered by using a conditional knockout approach.
Another miRNA that might be involved in neuronal survival is miR-8 (Karres et al., 2007), which is not brain-specific and has a complex pattern of expression. Mutant flies lacking miR-8 have limb and wing defects and increased apoptosis in the central nervous system. Moreover, concomitant decrease of the transcriptional repressor atrophin in miR-8 mutant flies partially rescued the phenotypes (Karres et al., 2007). It remains to be determined whether miR-8 contributes to neurodegeneration in mammals.
miRNAs in Neurodegenerative Diseases
Microarray studies have shown that the brain expresses a wide range of miRNAs, suggesting that these small RNA molecules participate in nervous system physiology (Lagos-Quintana et al., 2002; Miska et al., 2004; Lim et al., 2005; Manakov et al., 2009). More importantly, profiling studies revealed profound changes in several miRNAs (i.e., miR-9, miR-29 cluster, miR-107, miR-125b, and miR-128) in patient brains such as that of AD patients (Kim et al., 2007; Lukiw, 2007; Hebert et al., 2008; Johnson et al., 2008; Wang et al., 2008, 2011; Nunez-Iglesias et al., 2010). Although these global changes in miRNA expression under pathological conditions should be interpreted cautiously, they support the notion that dysregulation of miRNAs networks is a common theme in neurodegenerative diseases.
Alzheimer’s disease
A pathological hallmark of AD, the most prevalent neurodegenerative disease, is the accumulation of plaques formed by short β-amyloid (Aβ) peptides, commencing in the hippocampus, and spreading progressively throughout the brain (Ballard et al., 2011; Selkoe et al., 2012). It is likely the accumulation is caused by both increased production and impaired clearance of Aβ. Aβ peptides exert toxic effects and elicit an inflammatory response. Both may contribute to disrupt neuronal homeostasis and alter the integrity of neuronal networks involved in learning, memory, and other cognitive functions. Aβ peptides are generated through proteolytic cleavage of the amyloid precursor protein (APP) by γ-secretase and β-site APP-cleaving enzyme 1 (BACE1; O’Brien and Wong, 2011).
Amyloid precursor protein and BACE1 each contain several miRNA target sites in their 3′UTRs. Several miRNAs have been reported to repress APP expression, including miR-16, miR-101, miR-106a, and miR-520c (Patel et al., 2008; Liu et al., 2010; Long and Lahiri, 2011). miR-137 and miR-181c regulate serine palmitoyltransferase, which modulates the Aβ level (Geekiyanage and Chan, 2011). Interestingly, polymorphisms in miRNA binding sites in the 3′UTR of APP gene could influence the binding efficiency of these miRNAs. Thus, miRNAs might fine tune APP expression, which may enhance or limit the risk of AD (Delay et al., 2011).
A significant decrease in neuronal miR-107 expression and a parallel increase in BACE1 have been observed in AD patients (even those at the earliest stages of AD; Wang et al., 2008, 2011; Nelson and Wang, 2010). The 3′UTR of BACE1 mRNA has functional binding sites for miR-29 (Hebert et al., 2008), miR-107 (Wang et al., 2008), and miR-124 (Fang et al., 2012). Interestingly, miR-107 also controls the expression of other proteins relevant to AD pathology, such as cofilin (Yao et al., 2010), an actin-binding protein that accumulates in cytoplasmic inclusions known as Hirano bodies (Hirano, 1994). Thus, a single miRNA deregulation could activate multiple potentially pathogenic cascades upstream of Aβ accumulation.
Another possibility is that alterations in miRNAs in AD brain are a consequence of amyloid deposits. For instance, miR-106b is aberrantly expressed in APPswe/PSE9 mice (Wang et al., 2010a), and miR-146a levels are increased in AD brains and in several mouse models of AD (Li et al., 2011). In vitro exposure of hippocampal neurons to Aβ peptides preferentially decreases the levels of mature miRNAs (only a small fraction of miRNAs were upregulated; Schonrock et al., 2010). miRNAs were similarly deregulated in the hippocampus of APP23 mice at the onset of plaque formation. Overall, these studies suggest that miRNA deregulation is an essential pathogenic mechanism that is induced by Aβ aggregation and contributes to the progression and severity of AD.
Polyglutamine diseases
Polyglutamine (polyQ) diseases are a group of nine neurodegenerative disorders caused by an unstable CAG expansion in the coding region of their respective associated genes (Orr and Zoghbi, 2007). Apart from this common feature, polyQ diseases have distinct clinical presentations, and the proteins involved in these diseases have no structural/functional homology (Table 1). Huntington disease (HD), the most frequent polyQ disease, is characterized by the progressive loss of striatal neurons and motor impairment (typically resulting in the involuntary writhing movements called chorea) and is often associated with cognitive and behavioral deficits (Shoulson and Young, 2011).
Table 1
| miRNA | Disease | Type of evidence | Mechanism | Reference |
|---|---|---|---|---|
| miR-7 | PD | In vitro reporter assay | Regulation of α-synuclein | Doxakis (2010), Junn et al. (2009) |
| Overexpression in vitro | ||||
| miR-8 | miR-8 flies | Upregulation of atrophin | Karres et al. (2007) | |
| miR-9/9* | HD | Profiling studies | REST–coREST | Marti et al. (2010), Packer et al. (2008) |
| In vitro reporter assays | ||||
| miR-9/9* | ALS | Profiling in mouse mode | Neurofilament expression | Haramati et al. (2010) |
| miR-16 | AD | Profiling in mouse model | Regulation of APP levels | Liu et al. (2010) |
| Overexpression in vitro/in vivo | ||||
| miR-19 | SCA1 | In vitro reporter assays | Regulation of ataxin-1 | Lee et al. (2008) |
| In vitro gain of function | ||||
| miR-29 | AD | Profiling in patients | Regulation of BACE1 levels | Hebert et al. (2008) |
| In vitro reporter assay | ||||
| Overexpression/blocking in vitro | ||||
| miR-29b | FTD | In vitro reporter assay | Regulation of progranulin | Jiao et al. (2010) |
| Overexpression in vitro | ||||
| miR-34 | SCA3 overexpression in fly | Protective role | Liu et al. (2012) | |
| miR-101 | AD | In vitro reporter assays | Regulation of APP levels | Long and Lahiri (2011) |
| Overexpression/blocking in vitro | ||||
| miR-101 | SCA1 | In vitro reporter assays | Regulation of ataxin-1 | Lee et al. (2008) |
| In vitro gain of function | ||||
| miR-106a | AD | In vitro reporter assays | Regulation of APP levels | Patel et al. (2008) |
| Overexpression in vitro | ||||
| miR-106b | AD | Expression in mouse model | TGF-β | Wang et al. (2010a) |
| miR-107 | AD | Profiling in patients | Regulation of BACE1 levels | Wang et al. (2008) |
| In vitro reporter assay | ||||
| In situ hybridization in patients | ||||
| miR-107 | AD | In vitro reporter assay | Regulation of cofilin | Yao et al. (2010) |
| Levels in mouse models | ||||
| miR-107 | FTD | In vitro overexpression | Regulation of progranulin | Wang et al. (2010b) |
| miR-124 | miR-124-1 knockout mouse | Altered expression of Lhx2 | Sanuki et al. (2011) | |
| miR-124 | AD | Overexpression in vitro/in vivo | Regulation of BACE1 levels | Fang et al. (2012) |
| miR-130 | SCA1 | In vitro reporter assays | Regulation of ataxin-1 | Lee et al. (2008) |
| In vitro gain of function | ||||
| miR-133b | PD | Profiling in patients | ??? | Kim et al. (2007) |
| miR-137 | AD | Profiling in patients | Regulation of Aβ levels | Geekiyanage and Chan (2011) |
| In vitro reporter assay | ||||
| Blocking in vitro | ||||
| miR-144 | SCA1 | Profiling in patients | Regulation of ataxin-1 | Persengiev et al. (2011) |
| In vitro reporter assay | ||||
| miR-146a | AD | Profiling in patients | Downstream of Aβ | Li et al. (2011) |
| Expression in cell lines and mouse models | ||||
| miR-153 | PD | In vitro reporter assay | Regulation of α-synuclein | Doxakis (2010) |
| Overexpression in vitro | ||||
| miR-181c | AD | Profiling in patients | Regulation of Aβ levels | Geekiyanage and Chan (2011) |
| In vitro reporter assay | ||||
| Blocking in vitro | ||||
| miR-520c | AD | In vitro reporter assays | Regulation of APP levels | Patel et al. (2008) |
| Overexpression in vitro | ||||
| miR-659 | FTD–ALS | Human polymorphism | Regulation of progranulin | Rademakers et al. (2008) |
List of miRNAs and the neurodegenerative diseases to which they have been associated.
The causal mutation in HD is an expanded repetition of the CAG trinucleotide in the first exon of the gene encoding huntingtin (HTT; Gilliam et al., 1987), a large protein (3300 amino acids) whose functions remains mostly unknown. HTT associates with Ago2 in P-bodies, and HTT depletion impairs miRNA-mediated gene silencing (Savas et al., 2008). Expanded HTT may sequester RNA processing factors in the cytoplasm (Jiang et al., 2011).
miRNAs were implicated in HD pathogenesis by two lines of evidence. First, the levels of repressor element 1 silencing transcription (REST) factor, a major pathogenic pathway in HD (Buckley et al., 2010), is elevated in HD neurons, resulting in repression of hundreds of key neuronal genes (Zuccato et al., 2003, 2007; Johnson et al., 2010b). Canonical and non-canonical REST binding motifs have been mapped in close proximity to 22 miRNA sites in the human genome, including several miRNAs that are abundant in neurons (Bruce et al., 2004; Jothi et al., 2008; Yu et al., 2011). Could abnormal REST deregulate miRNAs network in HD patients? REST and its cofactor coREST possess functional target sites for miR-9 and miR-9*, respectively (Packer et al., 2008), and miR-9 and miR-9* (together with miR-7, miR-124, miR-132, and other miRNAs) are downregulated in HD patients (Johnson et al., 2008; Packer et al., 2008; Marti et al., 2010). miRNA deregulation in HD was confirmed by profiling studies in animal models, although there was a high degree of variability among the models (Lee et al., 2011). These observations strongly suggest that altered miRNA transcription is a major event in HD pathogenesis.
Besides HD, there are eight other polyQ diseases: dentatorubral–pallidoluysian atrophy, spinal and bulbar muscular atrophy, and spinocerebellar ataxia (SCA) 1, 2, 3, 6, 7, and 17 (Gatchel and Zoghbi, 2005). In fly models of SCA3, reduction of miRNA processing after knockout of Dicer1 markedly enhances the toxicity induced by mutant ataxin-3 (Bilen et al., 2006). In parallel genetic screens, a single miRNA, bantam, was identified as a potent downstream modulator of both polyQ and tau toxicity in flies (Bilen et al., 2006). In a recent paper, miR-34 was shown to be protective against expanded SCA3 (Liu et al., 2012). Subsequent work suggested that miR-19, miR-101, and miR-130 are important for the post-translational regulation of ataxin-1 (Lee et al., 2008). Inhibition of those miRNAs enhanced the cytotoxicity of polyQ-expanded ataxin-1 in human cells. Moreover, miR-144, a highly conserved miRNA, also regulated ataxin-1 expression and appeared to be associated with aging. Ataxin-1 levels are higher in the cerebellum and cortex of SCA1 patients than in healthy aged brains (Persengiev et al., 2011). On the other hand, ataxin-2 might be required for miRNA function (McCann et al., 2011), further supporting the intimate association between miRNAs and polyQ diseases.
Parkinson’s disease
Parkinson’s disease is a neurodegenerative disorder that primarily affects movement. Clinical symptoms include bradykinesia (decreased ability to start and continue movements), resting tremor, and rigidity. These symptoms are due to the relatively selective loss of dopaminergic neurons in the substantia nigra (Dauer and Przedborski, 2003) and reflect the impairment of neuronal networks important for regulating motor function. The past two decades have witnessed significant advances in the identification of distinct genetic loci at which pathogenic mutations are associated with parkinsonism (for review, see Lesage and Brice, 2009; Zimprich, 2011). Most research is focused on genes that have been conclusively linked to PD pathogenesis, including those encoding α-synuclein, leucine-rich repeat kinase 2, PTEN-induced putative kinase 1, parkin, and DJ-1.
Profiling studies of PD brains have revealed abnormalities in miRNA content (Kim et al., 2007; Minones-Moyano et al., 2011). One of the miRNAs found to be downregulated in these studies, miR-133b, plays a major role in the development of midbrain dopaminergic neurons by regulating the transcription factor Pitx3 (Kim et al., 2007). miR-7 and miR-153 control the expression of α-synuclein (Junn et al., 2009; Doxakis, 2010). Since intracellular levels of this protein appear to be critical in mediating its toxicity, deregulation of those miRNAs might lead to increased toxic levels of α-synuclein.
Amyotrophic lateral sclerosis and frontotemporal dementia
Amyotrophic lateral sclerosis (ALS) is a progressive, lethal, degenerative disorder characterized by the selective death of motor neurons in the brain and spinal cord (Pasinelli and Brown, 2006; Ferraiuolo et al., 2011). ALS shares many clinical, pathological, and molecular features with FTD, the second most common early-onset dementia (Ferrari et al., 2011). Clinically, FTD progresses from an insidious onset of behavioral changes, impaired frontal executive functions, and language deficits to more severe cognitive defects and, finally, to generalized dementia (Boxer and Miller, 2005). In familial FTD cases, the mutated locus has been identified in the genes encoding tau (Hong et al., 1998; Hutton et al., 1998), VCP (Watts et al., 2004), CHMP2B (Skibinski et al., 2005), progranulin (Baker et al., 2006; Cruts et al., 2006), and C9ORF72 (DeJesus-Hernandez et al., 2011; Renton et al., 2011). Among them, CHMP2B (Parkinson et al., 2006; Cox et al., 2010), VCP (Johnson et al., 2010a), and C9ORF72 (DeJesus-Hernandez et al., 2011; Renton et al., 2011; Stewart et al., 2012) have also been implicated in ALS. Moreover, the RNA-binding proteins TDP-43 and FUS have been strongly implicated in both FTD and ALS (Arai et al., 2006; Neumann et al., 2006, 2009; Sreedharan et al., 2008; Vance et al., 2009).
As described above, Dicer deletion in spinal motor neurons mimics most of the clinical (e.g., progressive paralysis) and pathological (e.g., astrocytosis and signs of axonopathy) features of ALS (Haramati et al., 2010). Whether the miRNA pathway is involved in the molecular pathogenesis of FTD/ALS caused by C9ORF72 repeat expansion is unknown. However, a potential involvement for the miRNA pathway in other forms of FTD or ALS comes from limited studies on TDP-43. TDP-43 is mutated in a subset of ALS patients (Sreedharan et al., 2008), and the expression levels of some miRNAs are affected in TDP-43 mutant flies (Buratti et al., 2010). Biochemical interactions between TDP-43 and Drosha, a key miRNA processing enzyme (Han et al., 2004), have been observed (Gregory et al., 2004; Ling et al., 2010). These findings raise the possibility that TDP-43 may play a role in miRNA processing; however, the mechanism remains to be elucidated and whether endogenous Drosha and TDP-43 physically interact needs to be demonstrated.
Some miRNAs are emerging as important contributors to ALS pathogenesis. The muscle-specific miR-206 is upregulated upon nerve injury and is required for regeneration of neuromuscular synapses. Moreover, miR-206 deficiency accelerates disease progression in a mouse model of ALS (Williams et al., 2009). miR-9/9*, an evolutionarily conserved and multifunctional miRNA (Yuva-Aydemir et al., 2011), is also potentially involved in ALS. Profiling of miRNA expression in motor neurons harboring an SMN1mut allele found in pediatric spinal motor atrophy revealed decreases of more than 90% in miR-9 and miR-9* levels (Haramati et al., 2010). More importantly, changes in the expression levels of the neurofilament subunits likely contribute to the disease, and miR-9 is an upstream regulator of the neurofilament mRNAs.
Several other miRNAs might be linked to FTD–ALS through different mechanisms. For example, miR-29b and miR-107 regulate progranulin levels (Jiao et al., 2010; Wang et al., 2010b). Since progranulin haploinsufficiency can cause FTD114,115, excessive levels of those miRNAs might decrease progranulin levels and be a risk factor for the disease. Consistent with these observations, a genetic polymorphism in the 3′UTR of the progranulin gene is associated with a higher risk of FTD–ALS, and multiple miRNAs are misregulated in FTD with TDP-43 pathology (Rademakers et al., 2008; Kocerha et al., 2011). This genetic variant (rs5848) affects the miR-659 binding site, resulting in more efficient binding and, consequently, decreased progranulin levels. It is not known whether translational regulation by miRNAs is a common mechanism in FTD caused by progranulin deficiency or in other neurodegenerative diseases (Rollinson et al., 2011).
Summary
Although much progress has been made in our understanding of how miRNAs control gene expression at the post-transcriptional level during development, their contributions to neurodegenerative disease remain poorly understood. Many fundamental questions need to be addressed. Is miRNA pathway disruption a downstream consequence or a cause of neurodegeneration (Figure 2 vs. Figure 3)? Are miRNAs essential for the proper regulation of aggregation-prone proteins or do they control additional pathogenic pathways? Are individual miRNAs especially important in particular neurodegenerative diseases? Which miRNA target or targets are relevant for the disease? To address these questions, it will be essential to generate novel experimental models, such as conditional knockouts in which developmental defects can be circumvented, allowing assessment of the functions of specific miRNAs in the adult brain. We expect many exciting findings will be made in years to come.
Figure 2
Figure 3
Statements
Acknowledgments
We thank S. Ordway for editorial assistance, A. Wilson for help with the figures, and Gao lab members for discussions. This work is supported by the National Institutes of Health (RO1NS066586 and RO1NS057553 to Fen-Biao Gao).
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
References
1
AhmedA.WicklundM. P. (2011). Amyotrophic lateral sclerosis: what role does environment play?Neurol. Clin.29, 689–711.10.1016/j.ncl.2011.06.001
2
AmbrosV.LeeR. C.LavanwayA.WilliamsP. T.JewellD. (2003). MicroRNAs and other tiny endogenous RNAs in C. elegans. Curr. Biol.13, 807–818.10.1016/S0960-9822(03)00287-2
3
AraiT.HasegawaM.AkiyamaH.IkedaK.NonakaT.MoriH.MannD.TsuchiyaK.YoshidaM.HashizumeY.OdaT. (2006). TDP-43 is a component of ubiquitin-positive tau-negative inclusions in frontotemporal lobar degeneration and amyotrophic lateral sclerosis. Biochem. Biophys. Res. Commun.351, 602–611.10.1016/j.bbrc.2006.10.093
4
BakerM.MackenzieI. R.Pickering-BrownS. M.GassJ.RademakersR.LindholmC.SnowdenJ.AdamsonJ.SadovnickA. D.RollinsonS.CannonA.DwoshE.NearyD.MelquistS.RichardsonA.DicksonD.BergerZ.EriksenJ.RobinsonT.ZehrC.DickeyC. A.CrookR.McGowanE.MannD.BoeveB.FeldmanH.HuttonM. (2006). Mutations in progranulin cause tau-negative frontotemporal dementia linked to chromosome 17. Nature442, 916–919.10.1038/nature05016
5
BallardC.GauthierS.CorbettA.BrayneC.AarslandD.JonesE. (2011). Alzheimer’s disease. Lancet377, 1019–1031.10.1016/S0140-6736(10)61349-9
6
BartelD. P. (2004). MicroRNAs: genomics, biogenesis, mechanism, and function. Cell116, 281–297.10.1016/S0092-8674(04)00045-5
7
BartelD. P. (2009). MicroRNAs: target recognition and regulatory functions. Cell136, 215–233.10.1016/j.cell.2009.01.002
8
BerezikovE.ThuemmlerF.van LaakeL. W.KondovaI.BontropR.CuppenE.PlasterkR. H. (2006). Diversity of microRNAs in human and chimpanzee brain. Nat. Genet.38, 1375–1377.10.1038/ng1794
9
BilenJ.LiuN.BurnettB. G.PittmanR. N.BoniniN. M. (2006). MicroRNA pathways modulate polyglutamine-induced neurodegeneration. Mol. Cell24, 157–163.10.1016/j.molcel.2006.07.030
10
BoxerA. L.MillerB. L. (2005). Clinical features of frontotemporal dementia. Alzheimer Dis. Assoc. Disord.19(Suppl. 1), S3–S6.10.1097/01.wad.0000183086.99691.91
11
BruceA. W.DonaldsonI. J.WoodI. C.YerburyS. A.SadowskiM. I.ChapmanM.GottgensB.BuckleyN. J. (2004). Genome-wide analysis of repressor element 1 silencing transcription factor/neuron-restrictive silencing factor (REST/NRSF) target genes. Proc. Natl. Acad. Sci. U.S.A.101, 10458–10463.10.1073/pnas.0401827101
12
BuckleyN. J.JohnsonR.ZuccatoC.BithellA.CattaneoE. (2010). The role of REST in transcriptional and epigenetic dysregulation in Huntington’s disease. Neurobiol. Dis.39, 28–39.10.1016/j.nbd.2010.02.003
13
BurattiE.De ContiL.StuaniC.RomanoM.BaralleM.BaralleF. (2010). Nuclear factor TDP-43 can affect selected microRNA levels. FEBS J.277, 2268–2281.10.1111/j.1742-4658.2010.07643.x
14
CaceresM.LachuerJ.ZapalaM. A.RedmondJ. C.KudoL.GeschwindD. H.LockhartD. J.PreussT. M.BarlowC. (2003). Elevated gene expression levels distinguish human from non-human primate brains. Proc. Natl. Acad. Sci. U.S.A.100, 13030–13035.10.1073/pnas.2135499100
15
CaoX.PfaffS. L.GageF. H. (2007). A functional study of miR-124 in the developing neural tube. Genes Dev.21, 531–536.10.1101/gad.1519207
16
ChendrimadaT. P.GregoryR. I.KumaraswamyE.NormanJ.CoochN.NishikuraK.ShiekhattarR. (2005). TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing. Nature436, 740–744.10.1038/nature03868
17
ChengL. C.PastranaE.TavazoieM.DoetschF. (2009). miR-124 regulates adult neurogenesis in the subventricular zone stem cell niche. Nat. Neurosci.12, 399–408.10.1038/nn.2240
18
CoxL. E.FerraiuoloL.GoodallE. F.HeathP. R.HigginbottomA.MortiboysH.HollingerH. C.HartleyJ. A.BrockingtonA.BurnessC. E.MorrisonK. E.WhartonS. B.GriersonA. J.InceP. G.KirbyJ.ShawP. J. (2010). Mutations in CHMP2B in lower motor neuron predominant amyotrophic lateral sclerosis (ALS). PLoS ONE5, e9872.10.1371/journal.pone.0009872
19
CrutsM.GijselinckI.van der ZeeJ.EngelborghsS.WilsH.PiriciD.RademakersR.VandenbergheR.DermautB.MartinJ. J.van DuijnC.PeetersK.SciotR.SantensP.De PooterT.MattheijssensM.Van den BroeckM.CuijtI.VennekensK.De DeynP. P.Kumar-SinghS.Van BroeckhovenC. (2006). Null mutations in progranulin cause ubiquitin-positive frontotemporal dementia linked to chromosome 17q21. Nature442, 920–924.10.1038/nature05017
20
CuellarT. L.DavisT. H.NelsonP. T.LoebG. B.HarfeB. D.UllianE.McManusM. T. (2008). Dicer loss in striatal neurons produces behavioral and neuroanatomical phenotypes in the absence of neurodegeneration. Proc. Natl. Acad. Sci. U.S.A.105, 5614–5619.10.1073/pnas.0801689105
21
DamianiD.AlexanderJ. J.O’RourkeJ. R.McManusM.JadhavA. P.CepkoC. L.HauswirthW. W.HarfeB. D.StrettoiE. (2008). Dicer inactivation leads to progressive functional and structural degeneration of the mouse retina. J. Neurosci.28, 4878–4887.10.1523/JNEUROSCI.0828-08.2008
22
DauerW.PrzedborskiS. (2003). Parkinson’s disease: mechanisms and models. Neuron39, 889–909.10.1016/S0896-6273(03)00568-3
23
DavisT. H.CuellarT. L.KochS. M.BarkerA. J.HarfeB. D.McManusM. T.UllianE. M. (2008). Conditional loss of Dicer disrupts cellular and tissue morphogenesis in the cortex and hippocampus. J. Neurosci.28, 4322–4330.10.1523/JNEUROSCI.4815-07.2008
24
De Pietri TonelliD.PulversJ. N.HaffnerC.MurchisonE. P.HannonG. J.HuttnerW. B. (2008). miRNAs are essential for survival and differentiation of newborn neurons but not for expansion of neural progenitors during early neurogenesis in the mouse embryonic neocortex. Development135, 3911–3921.10.1242/dev.025080
25
DeJesus-HernandezM.MackenzieI. R.BoeveB. F.BoxerA. L.BakerM.RutherfordN. J.NicholsonA. M.FinchN. A.FlynnH.AdamsonJ.KouriN.WojtasA.SengdyP.HsiungG. Y.KarydasA.SeeleyW. W.JosephsK. A.CoppolaG.GeschwindD. H.WszolekZ. K.FeldmanH.KnopmanD. S.PetersenR. C.MillerB. L.DicksonD. W.BoylanK. B.Graff-RadfordN. R.RademakersR. (2011). Expanded GGGGCC hexanucleotide repeat in noncoding region of C9ORF72 causes chromosome 9p-linked FTD and ALS. Neuron72, 245–256.10.1016/j.neuron.2011.09.011
26
DelayC.CalonF.MathewsP.HebertS. S. (2011). Alzheimer-specific variants in the 3′UTR of amyloid precursor protein affect microRNA function. Mol. Neurodegener.6, 70.10.1186/1750-1326-6-70
27
DoxakisE. (2010). Post-transcriptional regulation of alpha-synuclein expression by mir-7 and mir-153. J. Biol. Chem.285, 12726–12734.10.1074/jbc.M109.086827
28
EackerS. M.DawsonT. M.DawsonV. L. (2009). Understanding microRNAs in neurodegeneration. Nat. Rev. Neurosci.10, 837–841.
29
EnardW.KhaitovichP.KloseJ.ZollnerS.HeissigF.GiavaliscoP.Nieselt-StruweK.MuchmoreE.VarkiA.RavidR.DoxiadisG. M.BontropR. E.PaaboS. (2002). Intra- and interspecific variation in primate gene expression patterns. Science296, 340–343.10.1126/science.1068996
30
FangM.WangJ.ZhangX.GengY.HuZ.RuddJ. A.LingS.ChenW.HanS. (2012). The miR-124 regulates the expression of BACE1/beta-secretase correlated with cell death in Alzheimer’s disease. Toxicol. Lett.209, 94–105.10.1016/j.toxlet.2011.11.032
31
FenelonK.MukaiJ.XuB.HsuP. K.DrewL. J.KarayiorgouM.FischbachG. D.MacdermottA. B.GogosJ. A. (2011). Deficiency of Dgcr8, a gene disrupted by the 22q11.2 microdeletion, results in altered short-term plasticity in the prefrontal cortex. Proc. Natl. Acad. Sci. U.S.A.108, 4447–4452.10.1073/pnas.1101219108
32
FerraiuoloL.KirbyJ.GriersonA. J.SendtnerM.ShawP. J. (2011). Molecular pathways of motor neuron injury in amyotrophic lateral sclerosis. Nat. Rev. Neurol.7, 616–630.10.1038/nrneurol.2011.152
33
FerrariR.KapogiannisD.HueyE. D.MomeniP. (2011). FTD and ALS: a tale of two diseases. Curr. Alzheimer Res.8, 273–294.10.2174/156720511795563700
34
GaoF. B. (2010). Context-dependent functions of specific microRNAs in neuronal development. Neural Dev. 1, 5:25.10.1186/1749-8104-5-25
35
GaoH. M.HongJ. S. (2011). Gene-environment interactions: key to unraveling the mystery of Parkinson’s disease. Prog. Neurobiol.94, 1–19.10.1016/j.pneurobio.2011.03.005
36
GatchelJ. R.ZoghbiH. Y. (2005). Diseases of unstable repeat expansion: mechanisms and common principles. Nat. Rev. Genet.6, 743–755.10.1038/nrm1712
37
GeekiyanageH.ChanC. (2011). MicroRNA-137/181c regulates serine palmitoyltransferase and in turn amyloid beta, novel targets in sporadic Alzheimer’s disease. J. Neurosci.31, 14820–14830.10.1523/JNEUROSCI.3883-11.2011
38
GhildiyalM.ZamoreP. D. (2009). Small silencing RNAs: an expanding universe. Nat. Rev. Genet.10, 94–108.10.1038/nrg2504
39
GilliamT. C.TanziR. E.HainesJ. L.BonnerT. I.FaryniarzA. G.HobbsW. J.MacDonaldM. E.ChengS. V.FolsteinS. E.ConneallyP. M.WexlerN. S.GusellaJ. F. (1987). Localization of the Huntington’s disease gene to a small segment of chromosome 4 flanked by D4S10 and the telomere. Cell50, 565–571.10.1016/0092-8674(87)90029-8
40
GregoryR. I.YanK. P.AmuthanG.ChendrimadaT.DoratotajB.CoochN.ShiekhattarR. (2004). The Microprocessor complex mediates the genesis of microRNAs. Nature432, 235–240.10.1038/nature03120
41
HampelH.PrvulovicD.TeipelS.JessenF.LuckhausC.FrolichL.RiepeM. W.DodelR.LeyheT.BertramL.HoffmannW.FaltracoF. (2011). The future of Alzheimer’s disease: the next 10 years. Prog. Neurobiol.95, 718–728.10.1016/j.pneurobio.2011.05.012
42
HanJ.LeeY.YeomK. H.KimY. K.JinH.KimV. N. (2004). The Drosha-DGCR8 complex in primary microRNA processing. Genes Dev.18, 3016–3027.10.1101/gad.1262504
43
HaramatiS.ChapnikE.SztainbergY.EilamR.ZwangR.GershoniN.McGlinnE.HeiserP. W.WillsA. M.WirguinI.RubinL. L.MisawaH.TabinC. J.BrownR. J.ChenA.HornsteinE. (2010). miRNA malfunction causes spinal motor neuron disease. Proc. Natl. Acad. Sci. U.S.A.107, 13111–13116.10.1073/pnas.1006151107
44
HebertS. S.HorreK.NicolaiL.PapadopoulouA. S.MandemakersW.SilahtarogluA. N.KauppinenS.DelacourteA.De StrooperB. (2008). Loss of microRNA cluster miR-29a/b-1 in sporadic Alzheimer’s disease correlates with increased BACE1/beta-secretase expression. Proc. Natl. Acad. Sci. U.S.A.105, 6415–6420.10.1073/pnas.0710263105
45
HebertS. S.PapadopoulouA. S.SmithP.GalasM. C.PlanelE.SilahtarogluA. N.SergeantN.BueeL.De StrooperB. (2010). Genetic ablation of Dicer in adult forebrain neurons results in abnormal tau hyperphosphorylation and neurodegeneration. Hum. Mol. Genet.19, 3959–3969.10.1093/hmg/ddq311
46
HerranzH.CohenS. M. (2010). MicroRNAs and gene regulatory networks: managing the impact of noise in biological systems. Genes Dev.24, 1339–1344.10.1101/gad.1937010
47
HiranoA. (1994). Hirano bodies and related neuronal inclusions. Neuropathol. Appl. Neurobiol.20, 3–11.10.1111/j.1365-2990.1994.tb00951.x
48
HongM.ZhukarevaV.Vogelsberg-RagagliaV.WszolekZ.ReedL.MillerB. I.GeschwindD. H.BirdT. D.McKeelD.GoateA.MorrisJ. C.WilhelmsenK. C.SchellenbergG. D.TrojanowskiJ. Q.LeeV. M. (1998). Mutation-specific functional impairments in distinct tau isoforms of hereditary FTDP-17. Science282, 1914–1917.10.1126/science.282.5395.1914
49
HuH. Y.GuoS.XiJ.YanZ.FuN.ZhangX.MenzelC.LiangH.YangH.ZhaoM.ZengR.ChenW.PaaboS.KhaitovichP. (2011). MicroRNA expression and regulation in human, chimpanzee, and macaque brains. PLoS Genet.7, e1002327.10.1371/journal.pgen.1002327
50
HuttonM.LendonC. L.RizzuP.BakerM.FroelichS.HouldenH.Pickering-BrownS.ChakravertyS.IsaacsA.GroverA.HackettJ.AdamsonJ.LincolnS.DicksonD.DaviesP.PetersenR. C.StevensM.de GraaffE.WautersE.van BarenJ.HillebrandM.JoosseM.KwonJ. M.NowotnyP.CheL. K.NortonJ.MorrisJ. C.ReedL. A.TrojanowskiJ.BasunH.LannfeltL.NeystatM.FahnS.DarkF.TannenbergT.DoddP. R.HaywardN.KwokJ. B.SchofieldP. R.AndreadisA.SnowdenJ.CraufurdD.NearyD.OwenF.OostraB. A.HardyJ.GoateA.van SwietenJ.MannD.LynchT.HeutinkP. (1998). Association of missense and 5′-splice-site mutations in tau with the inherited dementia FTDP-17. Nature393, 702–705.10.1038/31508
51
HutvagnerG.McLachlanJ.PasquinelliA. E.BalintE.TuschlT.ZamoreP. D. (2001). A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA. Science293, 834–838.10.1126/science.1062961
52
IlievaH.PolymenidouM.ClevelandD. W. (2009). Non-cell autonomous toxicity in neurodegenerative disorders: ALS and beyond. J. Cell Biol.187, 761–772.10.1083/jcb.200908164
53
JiangY. J.CheM. X.YuanJ. Q.XieY. Y.YanX. Z.HuH. Y. (2011). Interaction with polyglutamine-expanded huntingtin alters cellular distribution and RNA processing of huntingtin yeast two-hybrid protein A (HYPA). J. Biol. Chem.286, 25236–25245.10.1074/jbc.M110.186494
54
JiaoJ.HerlL. D.FareseR. V.GaoF. B. (2010). MicroRNA-29b regulates the expression level of human progranulin, a secreted glycoprotein implicated in frontotemporal dementia. PLoS ONE5, e10551.10.1371/journal.pone.0010551
55
JohnsonJ. O.MandrioliJ.BenatarM.AbramzonY.Van DeerlinV. M.TrojanowskiJ. Q.GibbsJ. R.BrunettiM.GronkaS.WuuJ.DingJ.McCluskeyL.Martinez-LageM.FalconeD.HernandezD. G.ArepalliS.ChongS.SchymickJ. C.RothsteinJ.LandiF.WangY. D.CalvoA.MoraG.SabatelliM.MonsurroM. R.BattistiniS.SalviF.SpataroR.SolaP.BorgheroG.GalassiG.ScholzS. W.TaylorJ. P.RestagnoG.ChioA.TraynorB. J. (2010a). Exome sequencing reveals VCP mutations as a cause of familial ALS. Neuron68, 857–864.10.1016/j.neuron.2010.11.036
56
JohnsonR.RichterN.JauchR.GaughwinP. M.ZuccatoC.CattaneoE.StantonL. W. (2010b). The human accelerated region 1 noncoding RNA is repressed by REST in Huntington’s disease. Physiol. Genomics41, 269–274.10.1152/physiolgenomics.00019.2010
57
JohnsonR.ZuccatoC.BelyaevN. D.GuestD. J.CattaneoE.BuckleyN. J. (2008). A microRNA-based gene dysregulation pathway in Huntington’s disease. Neurobiol. Dis.29, 438–445.10.1016/j.nbd.2007.11.001
58
JothiR.CuddapahS.BarskiA.CuiK.ZhaoK. (2008). Genome-wide identification of in vivo protein-DNA binding sites from ChIP-Seq data. Nucleic Acids Res.36, 5221–5231.10.1093/nar/gkn488
59
JuckerM.WalkerL. C. (2011). Pathogenic protein seeding in Alzheimer disease and other neurodegenerative disorders. Ann. Neurol.70, 532–540.10.1002/ana.22615
60
JunnE.LeeK. W.JeongB. S.ChanT. W.ImJ. Y.MouradianM. M. (2009). Repression of alpha-synuclein expression and toxicity by microRNA-7. Proc. Natl. Acad. Sci. U.S.A.106, 13052–13057.10.1073/pnas.0906277106
61
KarresJ. S.HilgersV.CarreraI.TreismanJ.CohenS. M. (2007). The conserved microRNA miR-8 tunes atrophin levels to prevent neurodegeneration in Drosophila. Cell131, 136–145.10.1016/j.cell.2007.09.020
62
KimJ.InoueK.IshiiJ.VantiW. B.VoronovS. V.MurchisonE.HannonG.AbeliovichA. (2007). A MicroRNA feedback circuit in midbrain dopamine neurons. Science317, 1220–1224.10.1126/science.1146805
63
KingM. C.WilsonA. C. (1975). Evolution at two levels in humans and chimpanzees. Science188, 107–116.10.1126/science.1090005
64
KocerhaJ.KouriN.BakerM.FinchN.DeJesus-HernandezM.GonzalezJ.ChidamparamK.JosephsK. A.BoeveB. F.Graff-RadfordN. R.CrookJ.DicksonD. W.RademakersR. (2011). Altered microRNA expression in frontotemporal lobar degeneration with TDP-43 pathology caused by progranulin mutations. BMC Genomics12, 527.10.1186/1471-2164-12-527
65
KrolJ.LoedigeI.FilipowiczW. (2010). The widespread regulation of microRNA biogenesis, function and decay. Nat. Rev. Genet.11, 597–610.
66
Lagos-QuintanaM.RauhutR.YalcinA.MeyerJ.LendeckelW.TuschlT. (2002). Identification of tissue-specific microRNAs from mouse. Curr. Biol.12, 735–739.10.1016/S0960-9822(02)00809-6
67
LauP.de StrooperB. (2010). Dysregulated microRNAs in neurodegenerative disorders. Semin. Cell Dev. Biol.21, 768–773.10.1016/j.semcdb.2010.01.009
68
LeeE. B.LeeV. M.TrojanowskiJ. Q. (2012). Gains or losses: molecular mechanisms of TDP43-mediated neurodegeneration. Nat. Rev. Neurosci.13, 38–50.
69
LeeR. C.FeinbaumR. L.AmbrosV. (1993). The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell75, 843–854.10.1016/0092-8674(93)90529-Y
70
LeeS. T.ChuK.ImW. S.YoonH. J.ImJ. Y.ParkJ. E.ParkK. H.JungK. H.LeeS. K.KimM.RohJ. K. (2011). Altered microRNA regulation in Huntington’s disease models. Exp. Neurol.227, 172–179.10.1016/j.expneurol.2010.12.003
71
LeeY.AhnC.HanJ.ChoiH.KimJ.YimJ.LeeJ.ProvostP.RadmarkO.KimS.KimV. N. (2003). The nuclear RNase III Drosha initiates microRNA processing. Nature425, 415–419.10.1038/nature01916
72
LeeY.KimM.HanJ.YeomK. H.LeeS.BaekS. H.KimV. N. (2004). MicroRNA genes are transcribed by RNA polymerase II. EMBO J.23, 4051–4060.10.1038/sj.emboj.7600222
73
LeeY.SamacoR. C.GatchelJ. R.ThallerC.OrrH. T.ZoghbiH. Y. (2008). miR-19, miR-101 and miR-130 co-regulate ATXN1 levels to potentially modulate SCA1 pathogenesis. Nat. Neurosci.11, 1137–1139.10.1038/nn.2227
74
LesageS.BriceA. (2009). Parkinson’s disease: from monogenic forms to genetic susceptibility factors. Hum. Mol. Genet.18, R48–R59.10.1093/hmg/ddp012
75
LiY. Y.CuiJ. G.HillJ. M.BhattacharjeeS.ZhaoY.LukiwW. J. (2011). Increased expression of miRNA-146a in Alzheimer’s disease transgenic mouse models. Neurosci. Lett.487, 94–98.10.1016/j.neulet.2010.09.079
76
LimL. P.LauN. C.Garrett-EngeleP.GrimsonA.SchelterJ. M.CastleJ.BartelD. P.LinsleyP. S.JohnsonJ. M. (2005). Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs. Nature433, 769–773.10.1038/nature03315
77
LingS. C.AlbuquerqueC. P.HanJ. S.Lagier-TourenneC.TokunagaS.ZhouH.ClevelandD. W. (2010). ALS-associated mutations in TDP-43 increase its stability and promote TDP-43 complexes with FUS/TLS. Proc. Natl. Acad. Sci. U.S.A.107, 13318–13323.10.1073/pnas.1000315107
78
LiuN.LandrehM.CaoK.AbeM.HendriksG. J.KennerdellJ. R.ZhuY.WangL. S.BoniniN. M. (2012). The microRNA miR-34 modulates ageing and neurodegeneration in Drosophila. Nature482, 519–523.10.1038/nature10749
79
LiuW.LiuC.ZhuJ.ShuP.YinB.GongY.QiangB.YuanJ.PengX. (2010). MicroRNA-16 targets amyloid precursor protein to potentially modulate Alzheimer’s-associated pathogenesis in SAMP8 mice. Neurobiol. Aging33, 522–534.10.1016/j.neurobiolaging.2010.04.034
80
LongJ. M.LahiriD. K. (2011). MicroRNA-101 downregulates Alzheimer’s amyloid-beta precursor protein levels in human cell cultures and is differentially expressed. Biochem. Biophys. Res. Commun.404, 889–895.10.1016/j.bbrc.2010.12.053
81
LukiwW. J. (2007). Micro-RNA speciation in fetal, adult and Alzheimer’s disease hippocampus. Neuroreport18, 297–300.10.1097/WNR.0b013e3280148e8b
82
MaioranoN. A.MallamaciA. (2009). Promotion of embryonic cortico-cerebral neuronogenesis by miR-124. Neural Dev.4, 40.10.1186/1749-8104-4-40
83
ManakovS. A.GrantS. G.EnrightA. J. (2009). Reciprocal regulation of microRNA and mRNA profiles in neuronal development and synapse formation. BMC Genomics10, 419.10.1186/1471-2164-10-419
84
MartiE.PantanoL.Banez-CoronelM.LlorensF.Minones-MoyanoE.PortaS.SumoyL.FerrerI.EstivillX. (2010). A myriad of miRNA variants in control and Huntington’s disease brain regions detected by massively parallel sequencing. Nucleic Acids Res.38, 7219–7235.10.1093/nar/gkq575
85
McCannC.HolohanE. E.DasS.DervanA.LarkinA.LeeJ. A.RodriguesV.ParkerR.RamaswamiM. (2011). The Ataxin-2 protein is required for microRNA function and synapse-specific long-term olfactory habituation. Proc. Natl. Acad. Sci. U.S.A.108, E655–E662.10.1073/pnas.1107198108
86
Minones-MoyanoE.PortaS.EscaramisG.RabionetR.IraolaS.KagerbauerB.Espinosa-ParrillaY.FerrerI.EstivillX.MartiE. (2011). MicroRNA profiling of Parkinson’s disease brains identifies early downregulation of miR-34b/c which modulate mitochondrial function. Hum. Mol. Genet.20, 3067–3078.10.1093/hmg/ddr210
87
MiskaE. A.Alvarez-SaavedraE.TownsendM.YoshiiA.SestanN.RakicP.Constantine-PatonM.HorvitzH. R. (2004). Microarray analysis of microRNA expression in the developing mammalian brain. Genome Biol.5, R68.10.1186/gb-2004-5-9-r68
88
NelsonP. T.WangW. X. (2010). MiR-107 is reduced in Alzheimer’s disease brain neocortex: validation study. J. Alzheimers Dis.21, 75–79.
89
NeumannM.RademakersR.RoeberS.BakerM.KretzschmarH. A.MackenzieI. R. (2009). A new subtype of frontotemporal lobar degeneration with FUS pathology. Brain132, 2922–2931.10.1093/brain/awp044
90
NeumannM.SampathuD. M.KwongL. K.TruaxA. C.MicsenyiM. C.ChouT. T.BruceJ.SchuckT.GrossmanM.ClarkC. M.McCluskeyL. F.MillerB. L.MasliahE.MackenzieI. R.FeldmanH.FeidenW.KretzschmarH. A.TrojanowskiJ. Q.LeeV. M. (2006). Ubiquitinated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis. Science314, 130–133.10.1126/science.1134108
91
Nunez-IglesiasJ.LiuC. C.MorganT. E.FinchC. E.ZhouX. J. (2010). Joint genome-wide profiling of miRNA and mRNA expression in Alzheimer’s disease cortex reveals altered miRNA regulation. PLoS ONE5, e8898.10.1371/journal.pone.0008898
92
O’BrienR. J.WongP. C. (2011). Amyloid precursor protein processing and Alzheimer’s disease. Annu. Rev. Neurosci.34, 185–204.10.1146/annurev-neuro-061010-113613
93
OrrH. T.ZoghbiH. Y. (2007). Trinucleotide repeat disorders. Annu. Rev. Neurosci.30, 575–621.10.1146/annurev.neuro.29.051605.113042
94
PackerA. N.XingY.HarperS. Q.JonesL.DavidsonB. L. (2008). The bifunctional microRNA miR-9/miR-9* regulates REST and CoREST and is downregulated in Huntington’s disease. J. Neurosci.28, 14341–14346.10.1523/JNEUROSCI.2390-08.2008
95
ParkinsonN.InceP. G.SmithM. O.HighleyR.SkibinskiG.AndersenP. M.MorrisonK. E.PallH. S.HardimanO.CollingeJ.ShawP. J.FisherE. M. (2006). ALS phenotypes with mutations in CHMP2B (charged multivesicular body protein 2B). Neurology67, 1074–1077.10.1212/01.wnl.0000231510.89311.8b
96
PasinelliP.BrownR. H. (2006). Molecular biology of amyotrophic lateral sclerosis: insights from genetics. Nat. Rev. Neurosci.7, 710–723.10.1038/nrm2028
97
PatelN.HoangD.MillerN.AnsaloniS.HuangQ.RogersJ. T.LeeJ. C.SaundersA. J. (2008). MicroRNAs can regulate human APP levels. Mol. Neurodegener.3, 10.10.1186/1750-1326-3-10
98
PereiraJ. A.BaumannR.NorrmenC.SomandinC.MieheM.JacobC.LuhmannT.Hall-BozicH.ManteiN.MeijerD.SuterU. (2010). Dicer in Schwann cells is required for myelination and axonal integrity. J. Neurosci.30, 6763–6775.10.1523/JNEUROSCI.0801-10.2010
99
PersengievS.KondovaI.OttingN.KoeppenA. H.BontropR. E. (2011). Genome-wide analysis of miRNA expression reveals a potential role for miR-144 in brain aging and spinocerebellar ataxia pathogenesis. Neurobiol. Aging32, 2316.e17–2316.e27.10.1016/j.neurobiolaging.2010.03.014
100
PrinzM.PrillerJ.SisodiaS. S.RansohoffR. M. (2011). Heterogeneity of CNS myeloid cells and their roles in neurodegeneration. Nat. Neurosci.14, 1227–1235.10.1038/nn.2923
101
RademakersR.EriksenJ. L.BakerM.RobinsonT.AhmedZ.LincolnS. J.FinchN.RutherfordN. J.CrookR. J.JosephsK. A.BoeveB. F.KnopmanD. S.PetersenR. C.ParisiJ. E.CaselliR. J.WszolekZ. K.UittiR. J.FeldmanH.HuttonM. L.MackenzieI. R.Graff-RadfordN. R.DicksonD. W. (2008). Common variation in the miR-659 binding-site of GRN is a major risk factor for TDP43-positive frontotemporal dementia. Hum. Mol. Genet.17, 3631–3642.10.1093/hmg/ddn257
102
ReitzC.BrayneC.MayeuxR. (2011). Epidemiology of Alzheimer disease. Nat. Rev. Neurol.7, 137–152.10.1038/nrneurol.2011.2
103
RentonA. E.MajounieE.WaiteA.Simon-SanchezJ.RollinsonS.GibbsJ. R.SchymickJ. C.LaaksovirtaH.van SwietenJ. C.MyllykangasL.KalimoH.PaetauA.AbramzonY.RemesA. M.KaganovichA.ScholzS. W.DuckworthJ.DingJ.HarmerD. W.HernandezD. G.JohnsonJ. O.MokK.RytenM.TrabzuniD.GuerreiroR. J.OrrellR. W.NealJ.MurrayA.PearsonJ.JansenI. E.SondervanD.SeelaarH.BlakeD.YoungK.HalliwellN.CallisterJ. B.ToulsonG.RichardsonA.GerhardA.SnowdenJ.MannD.NearyD.NallsM. A.PeuralinnaT.JanssonL.IsoviitaV. M.KaivorinneA. L.Holtta-VuoriM.IkonenE.SulkavaR.BenatarM.WuuJ.ChioA.RestagnoG.BorgheroG.SabatelliM.HeckermanD.RogaevaE.ZinmanL.RothsteinJ. D.SendtnerM.DrepperC.EichlerE. E.AlkanC.AbdullaevZ.PackS. D.DutraA.PakE.HardyJ.SingletonA.WilliamsN. M.HeutinkP.Pickering-BrownS.MorrisH. R.TienariP. J.TraynorB. J. (2011). A hexanucleotide repeat expansion in C9ORF72 is the cause of chromosome 9p21-linked ALS-FTD. Neuron72, 257–268.10.1016/j.neuron.2011.09.010
104
RollinsonS.RohrerJ. D.van der ZeeJ.SleegersK.MeadS.EngelborghsS.CollingeJ.De DeynP. P.MannD. M.Van BroeckhovenC.Pickering-BrownS. M. (2011). No association of PGRN 3’UTR rs5848 in frontotemporal lobar degeneration. Neurobiol. Aging32, 754–755.10.1016/j.neurobiolaging.2009.04.009
105
SanukiR.OnishiA.KoikeC.MuramatsuR.WatanabeS.MuranishiY.IrieS.UneoS.KoyasuT.MatsuiR.CherasseY.UradeY.WatanabeD.KondoM.YamashitaT.FurukawaT. (2011). miR-124a is required for hippocampal axogenesis and retinal cone survival through Lhx2 suppression. Nat. Neurosci.14, 1125–1134.10.1038/nn.2897
106
SavasJ. N.MakuskyA.OttosenS.BaillatD.ThenF.KraincD.ShiekhattarR.MarkeyS. P.TaneseN. (2008). Huntington’s disease protein contributes to RNA-mediated gene silencing through association with Argonaute and P bodies. Proc. Natl. Acad. Sci. U.S.A.105, 10820–10825.10.1073/pnas.0800658105
107
SchaeferA.O’CarrollD.TanC. L.HillmanD.SugimoriM.LlinasR.GreengardP. (2007). Cerebellar neurodegeneration in the absence of microRNAs. J. Exp. Med.204, 1553–1558.10.1084/jem.20070823
108
SchofieldC. M.HsuR.BarkerA. J.GertzC. C.BlellochR.UllianE. M. (2011). Monoallelic deletion of the microRNA biogenesis gene Dgcr8 produces deficits in the development of excitatory synaptic transmission in the prefrontal cortex. Neural Dev.6, 11.10.1186/1749-8104-6-11
109
SchonrockN.KeY. D.HumphreysD.StaufenbielM.IttnerL. M.PreissT.GotzJ. (2010). Neuronal microRNA deregulation in response to Alzheimer’s disease amyloid-beta. PLoS ONE5, e11070.10.1371/journal.pone.0011070
110
SelkoeD.MandelkowE.HoltzmanD. (2012). Deciphering Alzheimer disease. Cold Spring Harb. Perspect. Med.2, a011460.
111
ShinD.ShinJ. Y.McManusM. T.PtacekL. J.FuY. H. (2009). Dicer ablation in oligodendrocytes provokes neuronal impairment in mice. Ann. Neurol.66, 843–857.10.1002/ana.21927
112
ShoulsonI.YoungA. B. (2011). Milestones in Huntington disease. Mov. Disord.26, 1127–1133.10.1002/mds.23685
113
SkibinskiG.ParkinsonN. J.BrownJ. M.ChakrabartiL.LloydS. L.HummerichH.NielsenJ. E.HodgesJ. R.SpillantiniM. G.ThusgaardT.BrandnerS.BrunA.RossorM. N.GadeA.JohannsenP.SorensenS. A.GydesenS.FisherE. M.CollingeJ. (2005). Mutations in the endosomal ESCRTIII-complex subunit CHMP2B in frontotemporal dementia. Nat. Genet.37, 806–808.10.1038/ng1609
114
SomelM.LiuX.TangL.YanZ.HuH.GuoS.JiangX.ZhangX.XuG.XieG.LiN.HuY.ChenW.PaaboS.KhaitovichP. (2011). MicroRNA-driven developmental remodeling in the brain distinguishes humans from other primates. PLoS Biol.9, e1001214.10.1371/journal.pbio.1001214
115
SonntagK. C. (2010). MicroRNAs and deregulated gene expression networks in neurodegeneration. Brain Res.1338, 48–57.10.1016/j.brainres.2010.03.106
116
SreedharanJ.BlairI. P.TripathiV. B.HuX.VanceC.RogeljB.AckerleyS.DurnallJ. C.WilliamsK. L.BurattiE.BaralleF.de BellerocheJ.MitchellJ. D.LeighP. N.Al-ChalabiA.MillerC. C.NicholsonG.ShawC. E. (2008). TDP-43 mutations in familial and sporadic amyotrophic lateral sclerosis. Science319, 1668–1672.10.1126/science.1154584
117
StarkK. L.XuB.BagchiA.LaiW. S.LiuH.HsuR.WanX.PavlidisP.MillsA. A.KarayiorgouM.GogosJ. A. (2008). Altered brain microRNA biogenesis contributes to phenotypic deficits in a 22q11-deletion mouse model. Nat. Genet.40, 751–760.10.1038/ng.138
118
StewartH.RutherfordN. J.BriembergH.KriegerC.CashmanN.FabrosM.BakerM.FokA.DeJesus-HernandezM.EisenA.RademakersR.MackenzieI. R. (2012). Clinical and pathological features of amyotrophic lateral sclerosis caused by mutation in the C9ORF72 gene on chromosome 9p. Acta Neuropathol.123, 409–417.10.1007/s00401-011-0937-5
119
TaoJ.WuH.LinQ.WeiW.LuX. H.CantleJ. P.AoY.OlsenR. W.YangX. W.ModyI.SofroniewM. V.SunY. E. (2011). Deletion of astroglial Dicer causes non-cell-autonomous neuronal dysfunction and degeneration. J. Neurosci.31, 8306–8319.10.1523/JNEUROSCI.0567-11.2011
120
VanceC.RogeljB.HortobagyiT.De VosK. J.NishimuraA. L.SreedharanJ.HuX.SmithB.RuddyD.WrightP.GanesalingamJ.WilliamsK. L.TripathiV.Al-SarajS.Al-ChalabiA.LeighP. N.BlairI. P.NicholsonG.de BellerocheJ.GalloJ. M.MillerC. C.ShawC. E. (2009). Mutations in FUS, an RNA processing protein, cause familial amyotrophic lateral sclerosis type 6. Science323, 1208–1211.10.1126/science.1165942
121
VerrierJ. D.Semple-RowlandS.MadorskyI.PapinJ. E.NotterpekL. (2010). Reduction of Dicer impairs Schwann cell differentiation and myelination. J. Neurosci. Res.88, 2558–2568.
122
VisvanathanJ.LeeS.LeeB.LeeJ. W.LeeS. K. (2007). The microRNA miR-124 antagonizes the anti-neural REST/SCP1 pathway during embryonic CNS development. Genes Dev.21, 744–749.10.1101/gad.1519107
123
WangH.LiuJ.ZongY.XuY.DengW.ZhuH.LiuY.MaC.HuangL.ZhangL.QinC. (2010a). miR-106b aberrantly expressed in a double transgenic mouse model for Alzheimer’s disease targets TGF-beta type II receptor. Brain Res.1357, 166–174.10.1016/j.brainres.2010.08.018
124
WangW. X.WilfredB. R.MadathilS. K.TangG.HuY.DimayugaJ.StrombergA. J.HuangQ.SaatmanK. E.NelsonP. T. (2010b). miR-107 regulates granulin/progranulin with implications for traumatic brain injury and neurodegenerative disease. Am. J. Pathol.177, 334–345.10.2353/ajpath.2010.091198
125
WangW. X.HuangQ.HuY.StrombergA. J.NelsonP. T. (2011). Patterns of microRNA expression in normal and early Alzheimer’s disease human temporal cortex: white matter versus gray matter. Acta Neuropathol.121, 193–205.10.1007/s00401-010-0756-0
126
WangW. X.RajeevB. W.StrombergA. J.RenN.TangG.HuangQ.RigoutsosI.NelsonP. T. (2008). The expression of microRNA miR-107 decreases early in Alzheimer’s disease and may accelerate disease progression through regulation of beta-site amyloid precursor protein-cleaving enzyme 1. J. Neurosci.28, 1213–1223.10.1523/JNEUROSCI.0750-08.2008
127
WattsG. D.WymerJ.KovachM. J.MehtaS. G.MummS.DarvishD.PestronkA.WhyteM. P.KimonisV. E. (2004). Inclusion body myopathy associated with Paget disease of bone and frontotemporal dementia is caused by mutant valosin-containing protein. Nat. Genet.36, 377–381.10.1038/ng1332
128
WilliamsA. H.ValdezG.MoresiV.QiX.McAnallyJ.ElliottJ. L.Bassel-DubyR.SanesJ. R.OlsonE. N. (2009). MicroRNA-206 delays ALS progression and promotes regeneration of neuromuscular synapses in mice. Science326, 1549–1554.10.1126/science.1181046
129
WittchenH. U.JacobiF.RehmJ.GustavssonA.SvenssonM.JonssonB.OlesenJ.AllgulanderC.AlonsoJ.FaravelliC.FratiglioniL.JennumP.LiebR.MaerckerA.van OsJ.PreisigM.Salvador-CarullaL.SimonR.SteinhausenH. C. (2011). The size and burden of mental disorders and other disorders of the brain in Europe 2010. Eur. Neuropsychopharmacol.21, 655–679.10.1016/S0924-977X(11)70328-5
130
WuD.RaafatA.PakE.ClemensS.MurashovA. K. (2012). Dicer-microRNA pathway is critical for peripheral nerve regeneration and functional recovery in vivo and regenerative axonogenesis in vitro. Exp. Neurol.233, 555–565.10.1016/j.expneurol.2011.11.034
131
YangJ. S.LaiE. C. (2011). Alternative miRNA biogenesis pathways and the interpretation of core miRNA pathway mutants. Mol. Cell43, 892–903.10.1016/j.molcel.2011.08.004
132
YaoJ.HennesseyT.FlyntA.LaiE.BealM. F.LinM. T. (2010). MicroRNA-related cofilin abnormality in Alzheimer’s disease. PLoS ONE5, e15546.10.1371/journal.pone.0015546
133
YuH. B.JohnsonR.KunarsoG.StantonL. W. (2011). Coassembly of REST and its cofactors at sites of gene repression in embryonic stem cells. Genome Res.21, 1284–1293.10.1101/gr.114488.110
134
Yuva-AydemirY.SimkinA.GasconE.GaoF. B. (2011). MicroRNA-9: functional evolution of a conserved small regulatory RNA. RNA Biol.8, 557–564.10.4161/rna.8.4.16019
135
ZimprichA. (2011). Genetics of Parkinson’s disease and essential tremor. Curr. Opin. Neurol.24, 318–323.10.1097/WCO.0b013e3283484b87
136
ZuccatoC.BelyaevN.ConfortiP.OoiL.TartariM.PapadimouE.MacDonaldM.FossaleE.ZeitlinS.BuckleyN.CattaneoE. (2007). Widespread disruption of repressor element-1 silencing transcription factor/neuron-restrictive silencer factor occupancy at its target genes in Huntington’s disease. J. Neurosci.27, 6972–6983.10.1523/JNEUROSCI.4278-06.2007
137
ZuccatoC.TartariM.CrottiA.GoffredoD.ValenzaM.ContiL.CataudellaT.LeavittB. R.HaydenM. R.TimmuskT.RigamontiD.CattaneoE. (2003). Huntingtin interacts with REST/NRSF to modulate the transcription of NRSE-controlled neuronal genes. Nat. Genet.35, 76–83.10.1038/ng1219
Summary
Keywords
Alzheimer’s disease, ALS, FTD, microRNAs, neurodegeneration, C9ORF72, CHMP2B, TDP-43
Citation
Gascon E and Gao F-B (2012) Cause or Effect: Misregulation of microRNA Pathways in Neurodegeneration. Front. Neurosci. 6:48. doi: 10.3389/fnins.2012.00048
Received
13 February 2012
Accepted
23 March 2012
Published
09 April 2012
Volume
6 - 2012
Edited by
Jernej Ule, Medical Research Council Laboratory of Molecular Biology, UK
Reviewed by
Tao Sun, Cornell University Weill Medical College, USA; Philip Wong, Johns Hopkins University School of Medicine, USA
Copyright
© 2012 Gascon and Gao.
This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.
*Correspondence: Eduardo Gascon and Fen-Biao Gao, Department of Neurology, University of Massachusetts Medical School, Worcester, MA 01605, USA. e-mail: eduardo.gascon@umassmed.edu; fen-biao.gao@umassmed.edu
This article was submitted to Frontiers in Neurogenomics, a specialty of Frontiers in Neuroscience.
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