This article is a correction to:
Acute Ablation of Cortical Pericytes Leads to Rapid Neurovascular Uncoupling
Kassandra Kisler1†
Angeliki M. Nikolakopoulou1†
Melanie D. Sweeney1Divna Lazic1,2
Zhen Zhao1
Berislav V. Zlokovic1*- 1Department of Physiology and Neuroscience, The Zilkha Neurogenetic Institute, Keck School of Medicine of the University of Southern California, Los Angeles, CA, United States
- 2Department of Neurobiology, Institute for Biological Research, University of Belgrade, Belgrade, Serbia
A corrigendum on
Acute ablation of cortical pericytes leads to rapid neurovascular uncoupling
by Kisler, K., Nikolakopoulou, A. M., Sweeney, M. D., Lazic, D., Zhao, Z., and Zlokovic, B. V. (2020). Front. Cell. Neurosci. 14:27. doi: 10.3389/fncel.2020.00027
In the original article, there was an error in the representative image in Figure 1B panel “9 d” as published. A representative image of a tissue section from a pericyte-CreER-iDTR mouse treated for 6 days with DT (6 d) was placed incorrectly into the 9 day (9 d) representative image location.
FIGURE 1
Figure 1. Ablation of cortical pericytes from the adult mouse brain leads to acute dysregulation of neurovascular coupling. (A) A diagram of the injection protocol of pericyte-CreER; iDTR mice with tamoxifen (TAM; 40 mg/kg daily for seven consecutive days), diphtheria toxin (DT; 0.1 mg per day for 10 consecutive days) or vehicle, and the time points when cerebral blood flow (CBF) responses to stimulus measured by laser Doppler flowmetry (LDF) and intrinsic optical signal (IOS) imaging, neuronal response to stimulus, and capillary density measurements were performed. (B) Representative confocal microscopy images of CD13-positive pericyte coverage of lectin-positive endothelial profiles in the S1 cortex hind-limb region at 3, 6, and 9 days of DT or vehicle administration, and 3 days post-DT or vehicle. Bar = 20 μm. (C) Quantification of pericyte coverage loss on capillaries (<6 μm in diameter) in the S1 cortex in TAM-treated pericyte-CreER; iDTR mice at 0, 3, 6, and 9 days of DT administration, and 3 days post-DT or vehicle treatment; n = 5 mice per group. *P < 0.05 vs. day 0 of DT treatment by analysis of variance (ANOVA) followed by Tukey's post hoc test. (D) CBF response to an electrical hind limb stimulus (60 s duration, 7 Hz, 2 ms pulse duration) in 3-month-old TAM-treated pericyte-CreER; iDTR mice determined by LDF in the S1 cortex hind-limb region at 0, 3, 6, and 9 days of DT or vehicle administration, and 3 days post-DT or vehicle treatment. CBF response is expressed as the percentage increase relative to baseline; n = 5 mice per group; *P < 0.05, by ANOVA followed by Tukey's post hoc test. (E) Pearson's correlation between CBF response to a stimulus as in (D) and loss of pericyte coverage determined at 3 days post-DT or vehicle treatment of TAM-treated pericyte-CreER; iDTR mice. Each point represents an individual response per mouse of 10 studied mice; P < 0.0001. Significance by two-tailed Pearson correlation; R, Pearson correlation coefficient. (F) Capillary (diameter <6 μm) density in the S1 cortex hind-limb region in TAM-treated pericyte-CreER; iDTR mice at 3 days post-DT or vehicle treatment; n = 3 mice per group. (G) LDF measurements of CBF response to endothelium-dependent vasodilator acetylcholine (10 μM) in TAM-treated pericyte-CreER; iDTR mice determined 3 days post-DT or vehicle treatment; n = 5 mice per group. Data in (C,D,F,G) represented as Mean ± SEM; in (F,G) ns, non-significant by Student's t-test. Circles denote individual values per mouse in (E–G).
The corrected Figure 1 and its caption is shown below.
The authors apologize for this error and state that it does not change scientific conclusions of the article in any way. The original article has been updated.
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Keywords: neurovascular coupling, acute pericyte ablation, cerebral blood flow, capillary, laser doppler flowmetry, intrinsic optical signal imaging, voltage-sensitive dye imaging
Citation: Kisler K, Nikolakopoulou AM, Sweeney MD, Lazic D, Zhao Z and Zlokovic BV (2022) Corrigendum: Acute ablation of cortical pericytes leads to rapid neurovascular uncoupling. Front. Cell. Neurosci. 16:1078919. doi: 10.3389/fncel.2022.1078919
Received: 24 October 2022; Accepted: 18 November 2022;
Published: 29 November 2022.
Edited and reviewed by: Eng-King Tan, National Neuroscience Institute (NNI), Singapore
Copyright © 2022 Kisler, Nikolakopoulou, Sweeney, Lazic, Zhao and Zlokovic. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Berislav V. Zlokovic, emxva292aWMmI3gwMDA0MDt1c2MuZWR1
†These authors have contributed equally to this work and share first authorship