Yuanlin Niu1,2†
Zhenjiang Liu
Bin Li- 1Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Institute of Animal Husbandry and Veterinary, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, China
- 2School of Public Health, Lanzhou University, Lanzhou, China
- 3National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, China
Polysaccharides derived from plants, algae, or fungi serve as the major components of some human diets. Polysaccharides have been shown to exhibit diverse biological activities in improving human health, and have also been proposed to function as potent modulators of gut microbiota composition, thus playing a bi-directional regulatory role in host health. Here, we review a variety of polysaccharide structures potentially linked to biological functions, and cover current research progress in characterizing their pharmaceutical effects in various disease models, including antioxidant, anticoagulant, anti-inflammatory, immunomodulatory, hypoglycemic, and antimicrobial activities. We also highlight the effects of polysaccharides on modulating gut microbiota via enrichment for beneficial taxa and suppression of potential pathogens, leading to increased microbial expression of carbohydrate-active enzymes and enhanced short chain fatty acid production. This review also discusses polysaccharide-mediated improvements in gut function by influencing interleukin and hormone secretion in host intestinal epithelial cells.
1. Introduction
Polysaccharides, formed by α- or β-glycosidic bond of identical or various monosaccharide monomers with10 or more polymerization, are naturally produced in large quantities by plants and fungi (Tan et al., 2017). To date, numerous polysaccharides have been shown to exhibit a range of biological activities, including anticoagulation, antiviral, antitumor, antioxidant, hypoglycemic, and immunomodulatory effects (Zeng et al., 2019; Zhang et al., 2019; Wang et al., 2019b; Kalinina et al., 2020; Chaisuwan et al., 2021; Chen C. et al., 2021; Kiddane and Kim, 2021; Liang Q. et al., 2021; Surayot et al., 2021; Figure 1 and Table 1). In addition, many studies have proposed that some polysaccharides also contribute to shaping the structure, diversity and function of gut microbiota and thus play a role in enhancing human health (Koropatkin et al., 2012; Chang et al., 2015; Lin et al., 2018; Zhang L. et al., 2018; Zhang X. et al., 2018; Chen G. et al., 2019; Ding et al., 2019; Wang M. et al., 2019; Wang Y. et al., 2020; Wang et al., 2020b; Guo et al., 2021a).
Figure 1. Bioactivities of polysaccharides (anticoagulation, antiviral, antitumor, antioxidant, hypoglycemic, anti-inflammatory and immunomodulatory). PI3K, phosphoinositide 3-kinase; AKT, serine/threonine-specific protein kinase; ERK, extracellular-signal-regulated kinase; MAPK, mitogen-activated protein kinase; NF-κB, nuclear factor-κB; ROS, reactive oxygen species; GSH-Px, glutathione peroxidase; SOD, superoxide dismutase; CAT, catalase; Nrf2, nuclear factor erythroid 2–related factor 2.
Table 1. The action of some bioactive polysaccharides in disease prevention and treatment.
Gut microbes share a symbiotic lifestyle with human hosts, colonizing the gastrointestinal tract, and a large number of studies have highlighted the significance of these microbes, collectively termed microbiota, in mediating interactions that determine human health or disease states involving the gastrointestinal, cardiovascular, orthopedic and even neurological systems (Björkstén et al., 2001; Li et al., 2019). Recently, naturally occurring undigestable polysaccharides in plants and foods were found to exert regulatory effects on gut microbiota by selecting for beneficial microorganisms in the gut while inhibiting the growth of pathogenic bacteria. This activity reinforces the structural and functional integrity of the intestinal mucosal barrier, and enhances the intestinal immune system via modulation of cytokine expression levels (de Vrese and Schrezenmeir, 2008; Chiu et al., 2014; Fuke et al., 2019; Han et al., 2020; Zhang et al., 2020; Zhao et al., 2020; Guo et al., 2021b). For example, Hawthorn HAW1-2 Polysaccharide (Guo et al., 2021b), Ziziphus Jujuba Polysaccharide (Han et al., 2020), glycyrrhiza polysaccharide (Zhang X. et al., 2018), and Lycium barbarum polysaccharide (Ding et al., 2019) were all reported to act as prebiotics by affecting gut microbiota structure and diversity. In addition, fungal polysaccharides were also found to play an important regulatory role as prebiotics through mechanisms similar to that of plant polysaccharides (Liang J. et al., 2021). However, studies of these various prebiotic effects of polysaccharides have yet to fully uncover the full range of interaction mechanisms between polysaccharides and gut microbiota. Thus, a summary of the different plant sources and structures of known bioactive polysaccharides can facilitate ongoing research efforts, especially their prebiotic effects and modulation of microbiota in the context of human health. It should be noted that progress toward understanding the diversity of prebiotic polysaccharide functions requires the integration of plant phenotypic data with multi-omics analyses to identify tripartite host-polysaccharide-microbiota interactions.
2. Overview of polysaccharides and their bioactivities
2.1. Structure and classification of polysaccharides
To provide an overview of higher order polysaccharide structures, polysaccharides are first categorized by their primary structure, determined by connection types, the organization and composition of sugar residues, the configuration of glycosidic bonds, and the conformation of sugar rings (Yang et al., 2020). The secondary structure of oligosaccharides, i.e., their regular conformation resulting from hydrogen bonding (the most common secondary bonds between main chains), is determined by the dihedral Φ, Ψ or ω angles of the polymer backbone (Hatcher et al., 2011). Based on the secondary structure of polysaccharides, the tertiary structure is formed by non-covalent interactions among carboxyl groups, hydroxyl groups, sulfate groups, and/or amino groups on the sugar units. The polysaccharide quaternary structure refers to aggregates formed by non-covalent bonding between polymer chains (Lafond et al., 2016). In addition, polysaccharides can be classified as either homopolysaccharides or heteropolysaccharides, homo-polysaccharides are composed of a single type of monosaccharide and hetero-polysaccharides are composed of different types of monosaccharides (Sinha and Kumria, 2001; Liu et al., 2008). For example, glucans are glucose homopolysaccharides, while mannans are mannose homopolysaccharides (d’Ayala et al., 2008). Overall, polysaccharides are categorized as glucans, mannans, pectin polysaccharides, arabinogalactans, galactans, fucoidan, fructan, and polyxylose, among others, based on their monosaccharide composition and linkage types (Tan et al., 2017; Maji, 2019; Figure 2).
Figure 2. Classification and composition of some representative polysaccharides and commonly polymerized monosaccharides. (A) Xylan (B) Beta-Glucan (C) Mannan (D) Arabinogalactan (E) Galactan (F) Fucoidan (G) Fructan.
Different monosaccharide contents and branching exhibit various bioactivities. Wu et al. (2020) isolated polysaccharide from the seeds of Litchi chinensis Sonn. and reported reduction of antitumor activity of branched backbone of polysaccharides with more than four monosaccharide units. The monosaccharide contents of polysaccharides could also influence their anti-inflammatory activity (Chen Y. C. et al., 2019). In addition, polysaccharides contain many functional groups, including hydroxyl groups and a hemiacetal reducing end that has the potential to reduce precursor salts (Park et al., 2011). Studies have revealed that the oxidation of polysaccharide hydroxyl groups to carbonyl groups play a significant role in reducing gold salts (Mata et al., 2009). Alternatively, the reducing end of polysaccharides has been used to introduce an amino functional group capable of forming complexes with and stabilizing metallic nanoparticles (Nadkarni et al., 1994). Carbohydrates with these amino groups bind strongly to the surface of gold or silver nanoparticles to provide a hydrophilic surface (Kemp et al., 2009). The introduction of specific functional groups will change a polysaccharide’s molecular weight, structure, types, position and number of substituents groups, and may alter the physiochemical and functional properties of that polysaccharide (Li et al., 2016). More specifically, various modifications, including sulfation, phosphorylation, carbomethylation, benzoylation or acetylation have all been shown to enhance the biology activity of some specific polysaccharides (Xu Y. et al., 2019). For instance, in heparan sulfate (HS) polysaccharides, negatively charged sulfate and carboxylate decorations can be organized into a variety of different so-called HS S-domains through a tightly controlled biosynthetic pathway that enables remarkable structural variability (Li and Kusche-Gullberg, 2016). Using standard DPPH and ABTS assays, Cao et al. (2020) characterized the antioxidant properties of sulfated polysaccharides from Amana edulis (Cao et al., 2020). Another study illustrated the positive effects of phosphorylation- based modifications on increasing the antioxidant activity of polysaccharides isolated from Momordica charantia (Chen F. et al., 2019). Recently, nanocellulose incorporated polysaccharides were reported to extent nanoparticle application in health promotion. Incorporation of chitosan and nanocellulose could improve their antimicrobial activity (Tomé et al., 2013), as well as exerting roles in wound healing (Hasan et al., 2017). These different combinations of functional groups of polysaccharides from diverse sources, along with monosaccharide composition, linkage types, and chemical modifications can thus result in different biological activities.
2.2. Polysaccharide bioactivities
2.2.1. Anticoagulation and anti-inflammatory effects
Polysaccharides are widely reported to exhibit anticoagulation properties by inhibiting thrombin activity, decreasing platelet counts, inhibiting platelet adhesion and aggregation, enhancing plasminase activity, and promoting the dissolution of fibrin (Matsubara et al., 2001; Pawlaczyk et al., 2011; Xue et al., 2018). Souza et al. (2015) identified polysaccharides from spinosa bark that could provide anticoagulant, antiplatelet and antithrombotic effects without increasing inducing a greater likelihood of hemorrhage (Souza et al., 2015). Similarly, an acidic polysaccharide was extracted from the edible mushroom Auricularia auricula which contained mannose, glucose, glucuronic acid and xylose oligosaccharides, but no sulfate ester links. This polysaccharide displayed potent anticoagulation effect by inhibiting thrombin via antithrombin activation (Yoon et al., 2003). Sulfated galactans produced by the seaweed Hypnea musciformis were also found to have antioxidant, anticoagulant, and immunostimulatory properties, depending on the method of their extraction (Gabriela das Chagas Faustino Alves et al., 2016).
Other than anticoagulant effects, polysaccharides are also known to exert anti-inflammatory activities. Fu et al. (2022) studied the structure of polysaccharides from Chinese aconite (Aconitum carmichaelii) leaves and demonstrated that these polysaccharides have immunomodulatory and anti-inflammatory effects on lipopolysaccharide (LPS)-induced inflammation in intestinal epithelial cells (Fu et al., 2022). In addition, in vitro investigation of the bioactivity of polysaccharides from Typha angustifolia using RAW264.7 cell provided evidence indicating that these polysaccharides can significantly suppress inflammatory cytokine production, nuclear factor-κB (NF-κB) signal pathway activation, and reactive oxygen species (ROS) production (Wei et al., 2021). Study on Sargassum fusiforme fucoidan has shown that polysaccharides can inhibit selectin-mediated leukocyte migration and infiltration by blocking interactions between P-selectin and its ligands on leukocytes, ultimately reducing the levels of IL-6, IL-8, TNF, and CRP cytokines to ameliorate systemic inflammation (Wu S. et al., 2019). Guo et al. (2018) studied the anti-inflammatory activity and related mechanism of polysaccharides isolated from Sargentodoxa cuneata. Their findings demonstrated that these polysaccharides could markedly inhibit carrageenan-induced edema in the hind paw of rats by decreasing malondialdehyde and prostaglandin E2 levels in the hind paw, serum and liver, while promoting SOD activity in serum and liver (Guo et al., 2018).
2.2.2. Immunomodulatory effects
Numerous polysaccharides from fungi and plants can reportedly provide various dietary and medicinal benefits, including marked effects on immune system function. Polysaccharides have also been shown to function as immunomodulators through a variety of mechanisms (Mousavi et al., 2015), such as activating macrophages, T cells, B lymphocytes, or natural killer cells, or by activating complements and promoting cytokine production (Kouakou et al., 2013). This regulation of innate immune response can substantially impact the host’s ability to rapidly respond to pathogens. As an essential component of the host immune system, macrophages collaborate with other cell types, such as neutrophils, to resist the adverse effects of biotic and abiotic stresses (Schepetkin and Quinn, 2006; Shen et al., 2014). To augment this function, some polysaccharide signal molecules can activate a macrophage-mediated immune response via binding with different receptors on macrophages, such as Toll-like receptor 4, complement receptor 3, scavenger receptor, mannose receptor, and Dectin-1, consequently initiating one or more intracellular signaling cascades that ultimately result in production of inflammation-related cytokines (Schepetkin and Quinn, 2006; Liao et al., 2015; Bunyatyan et al., 2017; Gong et al., 2017; Zhou et al., 2020). These macrophage-associated immunomodulatory effects of plant polysaccharides are largely mediated by increased ROS generation, cytokine secretion, cell proliferation, and phagocytic activity of macrophages (Gong et al., 2017) One earlier study described a novel polysacchaide obtained from the fruiting body of Dictyophora indusiate that could significantly promote macrophage secretion of NO, TNF-α, and IL-6 via complement receptor 3 in mouse RAW 264.7 cells (Liao et al., 2015). As organic selenium compound with complex chemical structure and diverse sources, selenium polysaccharide has been widely studied as its biological activities. A wide variety of non-specific immune cells, such as natural killer cells and macrophages display significantly improved immune function in the presence of selenium polysaccharides (Zhou et al., 2020). These advances in understanding the scope of polysaccharide activity suggest that many more plant polysaccharides with immunomodulatory effects have yet to be identified through extensive screening and research.
2.2.3. Hypoglycemic effects
Diabetes is a chronic, metabolic disease with typical hyperglycemia symptoms which is characterized by insulin resistance and a relative/absolute insulin insufficiency (Bunyatyan et al., 2017). Plant polysaccharides can also stimulate insulin secretion, modulate the activity of glucose metabolizing enzymes, inhibit the gluconeogenesis pathway, and promote glucose utilization in peripheral tissues, thus performing important functions in the prevention and treatment of diabetes (Wu et al., 2016). Tea made with guava (Psidium guajava L., Myrtaceae) leaves has long been used as a traditional herbal treatment for diabetes in Asia and North America (Oh et al., 2005). Polysaccharides from guava leaf have been shown to exhibit potent free-radical scavenging activity toward DPPH, OH, and ABTS, and can significantly lower fasting blood sugar, total cholesterol, total triglyceride, glycated serum protein, creatinine, and malonaldehyde levels (Luo et al., 2019). In addition, treatment with these polysaccharides can significantly increase total antioxidant activity and superoxide dismutase (SOD) enzyme activity in diabetic mice, consequently ameliorating damage to the liver, kidney, and pancreas (Luo et al., 2019). Recently, the application of bitter gourd (Momordica charantia) as herbal medicine/vegetable in the treatment against diabetes has been widely reported. One study investigating the hypoglycemic effects of Momordica charantia polysaccharides in alloxan-induced diabetic mice model showed that the polysaccharide treatment led to significantly lower fasting blood glucose levels and improved glucose tolerance, thus proposed dose-dependent anti-diabetic activity (Xu et al., 2015). In order to obtain better antidiabetic activities, a selenylated polysaccharide from Momordica charantia has been applied to diabetic mice and was reported to prevent pancreatic islets, liver and kidney damage from diabetes by reduction of fasting blood glucose levels, enhancement of insulin levels and antioxidant enzyme activities (Ru et al., 2020). The pumpkin polysaccharides also demonstrated a significant glucose tolerance effect, and effectively alleviated insulin resistance in addition to providing cytoprotective benefits on type II diabetes mellitus (T2DM) mice (Chen X. et al., 2019). In particular, glucomannan and glucogalactan have been shown to exhibit significant antidiabetic properties by inhibiting alpha-amylase and alpha-glucosidase activity to promote pancreatic beta cell proliferation and stimulate insulin sensitivity and secretion (Mirzadeh et al., 2021).
Mechanistically, these effects are mediated by phosphorylated tyrosine residues present in the intracellular substrates of the insulin receptor (IRS) (Mirzadeh et al., 2021). These substrates could activate the phosphoinositide 3-kinase (PI3K), phosphoinositide-dependent protein kinase 1 and 2 (PDK1/2) and then activates serine/threonine-specific protein kinase (AKT) pathways (Ganesan and Xu, 2019). AKT activates the phosphorylation of glycogen synthase kinase 3 (GSK3), resulting in upregulation of glycogen synthesis in the liver and skeletal muscle (Mirzadeh et al., 2021). Moreover, AKT can also stimulate the translocation of glucose transporter 4 (GLUT4) to the plasma membrane which consequently enhance glucose uptake (Wang et al., 2012). Study also found that AkT is a main mediator in activating the extracellular-signal-regulated kinase (ERK) /mitogen-activated protein kinase (MAPK) pathways thereby triggering several physiological and biochemical mechanisms, such as cell differentiation, proliferation, apoptosis, and cell endurance (Jayachandran et al., 2019). IRS studies on Ophiopogon japonicus polysaccharide and glucopyranose-rich heteropolysaccharide from Catathelasma ventricosum demonstrated that these compounds could trigger the PI3K/AKT signaling pathway through IRS1, PI3K-p85, and phosphorylated AKT to promote insulin sensitivity and improve diabetes-associated renal disease (Wang et al., 2012; Liu et al., 2016a,b). Collectively, polysaccharide could regulate glucose uptaking, glycogen synthesis and β-cell activity through PI3K/Akt pathway and ERK/MAPK pathways and resulting in playing an anti-diabetic role (Figure 3).
Figure 3. The hypoglycemic function of bioactive polysaccharides on the insulin signaling pathway. IRS, insulin receptor substrate; PI3K, phosphoinositide 3-kinase; PDK1/2, phosphoinositide-dependent protein kinase 1 and 2; AKT, serine/threonine-specific protein kinase; GSK-3, glycogen synthase kinase-3; GLUT4, glucose transporter type 4; ERK, extracellular-signal-regulated kinase; MAPK, mitogen-activated protein kinase.
2.2.4. Antioxidant effects
Oxidative stress can be link to a variety of diseases including cancer, cardiovascular diseases, diabetes, respiratory diseases, immune deficiency and neurodegenerative disorders, while antioxidants could protect cells against free radicals and reduce the risk of many diseases (Fridovich, 1999; Fang et al., 2002). Plant polysaccharides have been shown to directly eliminate free radicals by inhibiting lipid peroxidation, scavenging hydroxyl free radicals, and clearing superoxide anion free radicals. They also act on free radicals indirectly by enhancing the activities of SOD, catalase (CAT), and glutathione peroxidase to maintain a balance of free radicals, which can collectively diminish or avert the occurrence of disease (Xie et al., 2016). Lin et al. (2022) used enzymatic and microwave extraction methods to obtain polysaccharides from Purple-Heart Radish (Raphanus sativus) that displayed high antioxidant effects by inhibiting lipid peroxidation (Lin et al., 2022). Studies of polysaccharides from yerba mate (Ilex paraguariensis) tea reported IC50 values of 3.36 ± 0.31 mg/mL for ·OH scavenging activity, suggesting a strong antioxidant capacity (Chen X. et al., 2019). Xu et al. (2012) characterized polysaccharides from flowers of Camellia sinensis and found a high, dose-dependent capacity for scavenging superoxide anion free radicals (Xu et al., 2012). Study of polysaccharides from Astragalus membranaceus (Fisch.) has shown that ROS levels decrease, SOD activity increases, and superoxide dismutase free radical scavenging is enhanced, which can alleviate tissue damage and delay senescence (Song et al., 2019). Shan et al. (2011) found that administering Lycium barbarum polysaccharides led to significantly higher SOD and glutathione peroxidase (GPX) levels in rats with exercise-induced oxidative stress, indicating that these polysaccharides played a significant role in preventing oxidative stress after exhaustive exercise (Shan et al., 2011). Investigation of alfalfa polysaccharides illustrated their antioxidant effects on immune response in preventing H2O2-induced oxidative damage by activating mitogen-activated protein kinase (MAPK)/nuclear factor erythroid 2–related factor 2 (Nrf2) signaling pathways while suppressing NF-κB signaling in mouse embryonic fibroblasts (Wang et al., 2019a).
In addition to naturally occurring polysaccharides, chemical modification can also enhance the antioxidant effects of some polysaccharides. For example, findings by Gao et al. (2017) showed that selenylation of Angelica sinensis polysaccharide could enhance its antioxidant and hepatoprotective activity through inhibition of p- ERK and p-JNK signaling in mice with hepatic injury (Gao et al., 2017). Collectively, polysaccharides from diverse sources have been applied as functional antioxidant components in many pharmaceutical/nutraceutical products due to their capacity for modulating ROS levels, enzymatic and non-enzymatic antioxidant defense responses (e.g., SOD, CAT, GPX), and oxidative stress-induced signaling pathways (e.g., MAPK ERK, and JNK).
2.2.5. Antitumor and antiviral capacities
Cancer remains one of the greatest threats to public health worldwide, and is a long-standing focus of research attention and drug development, especially those with reduced side-effects. Bioactive polysaccharides have been identified that exert anti-tumor activity toward a variety of tumor cells without inducing cytotoxicity in normal cells (You et al., 2013). In the cactus (Opuntia dilleniid), polysaccharides were found that can induce S-phase arrest and block the growth of SK-MES-1 cells, possibly through increased levels of P53 and phosphatase and tensin homolog deleted on chromosome 10 protein (PTEN) (Li et al., 2014). The screening and discovery of new anti-tumor polysaccharides represents an ongoing pursuit for many research groups, with the most common direct tumor-killing mechanisms of anti-tumor polysaccharides involving cell cycle arrest, blocking angiogenesis, and inducing apoptosis. In addition, some polysaccharides act through immunomodulation to indirectly induce tumor killing (Khan T. et al., 2019; Lu et al., 2020).
Several studies have described antiviral effects of both natural and chemically modified polysaccharides. For example, Witvrouw et al. (1994) observed antiviral activity by a sulfated polysaccharide isolated from the red seaweed (Aghardhiella tenera) in vitro and found that it could inhibit the cytopathic effects of human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2), as well as against other enveloped viruses. Examination of the inhibitory effects of Glycyrrhiza polysaccharide (GPS) on bovine immunodeficiency virus (BIV), adenovirus type III (AdVIII), and coxsackie virus (CBV3) revealed that GPS could inhibit BIV to some extent, but showed obvious inhibition or direct inactivation of AdVIII and CBV3 (Wang Y. et al., 2020). Further technological advances in plant polysaccharide research will enable more comprehensive screening for effective anti-tumor and antiviral polysaccharide drugs.
3. Prebiotic effects of various sources of polysaccharides on gut microbiota
The adult human intestinal tract harbors an estimated stable community of 39 trillion microbial cells, which has been recognized as a diverse and dynamic ecosystem containing bacteria, fungi, protozoa, and viruses (Rajakovich and Balskus, 2019). Gut bacterial communities are generally comprised of six major phyla, including Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, Verrucomicrobia, and Fusobacteria (Gong et al., 2020), which collectively contribute to protecting the intestinal barrier, preventing pathogen invasion, participating in vitamin synthesis (Vitamin K, Vitamin B), host metabolism, and other functions related to nutrient uptake (Cresci and Bawden, 2015). In addition, mutual interactions between gut microbiota and their host were found to provide important functions in host health, and dysbiosis in gut microbiota has been linked to inflammatory bowel disease, obesity, allergies, and psychological disorders (Coyte et al., 2015). Patterson et al. (2016) proposed a possible association between gut microbiota and obesity and metabolic syndrome, which are both accompanied by an increased ratio of Firmicutes to Bacteroidetes in obese phenotypes patients, compared with that in non-obese individuals (Patterson et al., 2016). Other studies have indicated that gut microbiota can influence the development of neurological disorders as autism, depression, Alzheimer’s disease, and Parkinson’s disease (Dinan and Cryan, 2017).
Several factors, including infant delivery modes and feeding patterns, host, diet, antibiotic usage, and other factors have all been reported to affect gut microbiota composition and diversity (Cresci and Bawden, 2015). Among these factors, host and diet exert a particularly strong influence on gut microbiota. Our own previous study identified clear differences in gut microbiota driven by dietary variation among people living in close regional proximity (Liu et al., 2020). Furthermore, dietary intervention was recently recognized as a reliable strategy for altering gut microbiota and enhancing gut function, especially through prebiotics. Prebiotics are food components that may promote health in the host by exert activity in the gastrointestinal microbiota, such as fructose oligosaccharides, arabino-oligosaccharides, isomalt oligosaccharides, polyphenols and polyunsaturated fatty acids and etc. (Gibson et al., 2004, 2017). Among these prebiotics, dietary polysaccharides are relatively large macromolecules that are difficult to digest and absorbed, but can serve as carbon sources that potentially enrich beneficial gut microbes, or regulate microbial growth, activity, and metabolite production (Koropatkin et al., 2012; Chang et al., 2015; Wang et al., 2018). Furthermore, the polysaccharides affected gut microbiota modulation via enrichment for beneficial taxa and suppression of potential pathogens, leading to increased microbial expression of carbohydrate-active enzymes and enhanced short chain fatty acid production. In addition, polysaccharide mediated improvements in gut function such as influencing immune-related cytokines and hormone secretion in host intestinal epithelial cells and maintaining gut barrier function (Figure 4).
Figure 4. Prebiotic effects of polysaccharides toward gut microbial modulation. LPS, lipopolysaccharide; VH/CD, villus height/crypt depth; CAZymes, carbohydrate-active enzymes; SCFA, short-chain fatty acids; PPARγ, peroxisome proliferator-activated receptor-γ; PYY, peptide tyrosine; IL-2, interleukin-2; IFN-γ, interferon-γ; TNF-α, tumor necrosis factor-α.
Plant polysaccharides have been frequently proposed to serve as regulators of gut microecology (Fang et al., 2019), and have been shown to regulate the diversity and structure of gut microbiota, thus altering metabolic functions in the gastrointestinal tract (Zhao et al., 2021). Oral administration of polysaccharides from Ziziphus Jujuba cv. Pozao in cyclophosphamide-induced mice led to enrichment of Firmicutes and decreased Bacteroidetes, with genus-level increases in the relative abundance of Bacteroidales-S24-7-group, Lachnospiraceae, Alloprevotella, Alistipes, and Bacteroides (Han et al., 2020). Chen and colleagues showed that Sarcodon aspratus polysaccharides could serve as a prebiotic treatment to prevent obesity in mice fed with a high-fat diet, and led to increased relative abundance of Lactobacillus, Bacteroides and Akkermansia, decreased Firmicutes-to-Bacteroidetes ratio, inhibitory effects on immune cells activation and adipocyte differentiation in adipose tissues (Chen et al., 2020). Poria cocos polysaccharides were shown to alleviate intestinal mucosal injury, improve intestinal integrity, and restore the composition and structure of gut microbiota following dysbiosis by promoting the proliferation of beneficial bacteria in Ob/Ob Mice (Sun et al., 2019). Li et al. (2020) investigated the hypoglycemic and hypolipidemic effects of tea polysaccharides, potentially mediated by changes in gut microbiota and metabolism, in a rat model of type 2 diabetes. They stated that treatment with tea polysaccharides restored some specific bacterial taxa, such as Lachnospira, Victivallis, Roseburia, and Fluviicola in diabetic rats, whereas Bacteroides was decreased (Li et al., 2020). Taken together, polysaccharides play an important role in improving body health by increasing the enrichment of beneficial bacteria and reducing the proximity of harmful bacteria.
In addition, gut microbiota could produce short-chain fatty acids (SCFAs) and other metabolites, such as acetic acid, propionic acid, butyric acid, lactic acid and succinic acids, etc. These SCFAs can, in turn, affect gut functions to jointly regulate health (Wu Y et al., 2019). SCFAs are the final product of polysaccharide fermentation by gut microbiota (Mitsou et al., 2020), and are well-known to contribute to maintaining epithelial cell growth in the colon, modulate host metabolism, participate in immune regulation of the intestinal system, and play an indispensable role in maintaining the homeostasis of human gut microbiota (Morrison and Preston, 2016). In a study by Guo et al., treatment with polysaccharide from hawthorn (Crataegus monogyna) by gavage ameliorated inflammation symptoms in mice with dextran sulfate sodium (DSS)-induced colitis through significantly increased production of total SCFAs and acetic acid, attributable to greater relative abundance of Alestipes and Odoribacter (Guo et al., 2021b). Investigation of Cyclocarya paliurus polysaccharides in type 2 diabetic rat models. Suggested that these molecules could enrich for SCFA-producing bacteria, leading to elevated SCFA production and upregulation of associated sensory mediators that alleviate symptoms of type 2 diabetes (Yao et al., 2020). β-glucans, inulin, and oligofructose have all been found to significantly increase butyric acid production, which serve as a nutrient source for intestinal epithelial cells, reduces pH in the lumen, and provides energy for human activities (Song et al., 2014; Kim and Jazwinski, 2018), whereas polysaccharides such as α-glucan, fructan, and arabinoxylan can reportedly increase intestinal acetic acid levels (Shang et al., 2018). Insoluble polysaccharide isolated from the sclerotium of Poria cocos, as a prebiotic, increased the abundance of butyrate-producing bacteria such as Lachnospiracea and Clostridium, leading to an increase in the level of butyrate and improvement of gut mucosal integrity and activated the intestinal PPAR-γ pathway, significantly improving glucose and lipid metabolism and alleviating hepatic steatosis in ob/ob mice, suggesting its potential for the treatment of metabolic diseases (Sun et al., 2019). As one of the important natural polysaccharides source, marine polysaccharides were also well studied recently. Lentinan and sea anemone polysaccharides were recently reported to facilitate the production of total SCFAs, maintain gut homeostasis, and provide energy (Wang et al., 2018). In addition, polysaccharides from tea, C. sinensis, can also reduce blood glucose and lipid levels, promote SCFA production, attenuate insulin resistance, confer protective effects against pancreatic damage in type 2 diabetic rat model (Li et al., 2020).
In addition to regulating SCFA production, study showed that plant polysaccharides can up-regulate the expression of genes encoding carbohydrate-active enzymes (CAZymes), enhancing CAZyme activity and thus leads to higher SCFA production and increased tight junction protein expression with concurrent suppression of metabolic endotoxemia and decreased expression of inflammatory factors (Nguyen et al., 2016). Guo et al. (2021a) reported that Ganoderma lucidum polysaccharide could also upregulate CAZyme expression, especially glycoside hydrolases, polysaccharide lyases, glycosyltransferases, and carbohydrate esterases, leading to improved health.
Furthermore, polysaccharides can facilitate repair of damaged intestinal barrier to ensure the integrity of intestinal structures (Liang J. et al., 2021). Zhou et al. (2021) conducted co-treatment of American ginseng polysaccharide and ginsenoside altered uric acid, xanthurenic acid, acylcarnitine and restored the morphology of intestine. Specifically, the co-treatment resulted in an up-regulation of the villus height (VH)/crypt depth (CD) ratio, as well as an increase in the areas of mucins expression, quantity of goblet cells and expression of tight junction proteins (ZO-1, occludin) and then protecting the intestinal barrier (Zhou et al., 2021). Additionally, study of Yu et al. (2021) found that Cyclocarya paliurus polysaccharides dramatically increased the intestine antioxidant defense of CTX-induced mice, repaired the intestinal barrier by restoring the length of villi and depth of crypt, up-regulating the expression of tight junction proteins, shifting the composition and diversity of the gut microbiota, and regulating the relative abundances of specific taxa, then restoring intestinal mucosal barrier function (Yu et al., 2021).
Additionally, polysaccharide mediated improvements in gut function such as influencing immune-related cytokines and hormone secretion. Study of Lycium barbarum polysaccharide also suggested that polysaccharide treatment could protect immune organs, enhance the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α, and IFN-γ) and prevent the hepatotoxicity in cyclophosphamide (CTX)-induced mice (Ding et al., 2019). In immunosuppressed mice, Cordyceps sinensis polysaccharide was found to modulate gut microbiota, alleviate gut injury, and regulate the balance of T helper (Th)1/Th2 cells (Chen S. et al., 2021). Khan I. et al. (2019) reported that the treatment of Ganoderma lucidum polysaccharides markedly promoted SCFA produced bacteria and abridged sulfate-reducing bacteria in a time-dependent manner, altered expressions of histone deacetylases, anti-cancer gut hormone PYY, and PPAPγ in ApcMin/+ mice. Collectively, both in vitro and animal experiments applied for polysaccharide from different sources has been proved to have gut modulation capacity as prebiotic, however, clinical application of well recognized polysaccharides could help further understanding the actual response of human gut microbiota. These studies collectively indicate that plant polysaccharides share a complex relationship with gut microbes, and further study is required to reveal the full scope of mechanisms. In addition, gut regulation by postbiotic as secreted polysaccharides and extracellular polysaccharides has aroused attention recently. In several studies, the lactic acid bacteria-exopolysaccharides showed anti-oxidative and immunomodulatory activities as well as gut microbiota regulation effect (Xu R. et al., 2019; Xu Y. et al., 2019; Nataraj et al., 2020; Wang et al., 2020a). Further research into the biological activities of these metabolites is expected to reveal novel uses for postbiotics in medicine and beyond.
In general, the influence of polysaccharides on gut microbes can be summarized in three main aspects. Firstly, polysaccharides can attenuate damage to intestinal mucosa, alleviate intestinal inflammation, facilitate repair of damaged intestinal barrier, and ensure the integrity of the intestinal structure. Second, polysaccharides can alter intestinal microbial community composition and function, enriching beneficial bacteria while suppressing the proliferation of potential pathogens, consequently improving the relative content of various intestinal metabolites, especially SCFAs, that positively impact overall health. Third, polysaccharides can directly regulate gut function, such as modulating the secretion of interleukins and hormones in intestinal epithelial cells, or activating microbial CAZyme expression, which can reduce the likelihood of gut disease. However, most of these interventional studies are based on in vitro experiments or model animals, and thus clinical trials examining the effects of polysaccharide-based treatments in gut dysbiosis could improve our understanding of the interactions between polysaccharides, gut microbiota, and gastrointestinal function in humans.
4. Conclusion
Dietary polysaccharides have been shown to exert a range of biological activities, including anticoagulation, anti-inflammatory, immunomodulatory, hypoglycemic, antitumor, and antioxidant effects that impact gut health, in addition to modulating gut function and microbiota composition. These diverse functions are largely dependent on the primary structure and functional groups of the saccharide monomers. Ongoing innovations in data mining and compound screening continually expand the range of available bioactive polysaccharides, and drive the development of their pharmacological applications. It is noteworthy that these bioactive polysaccharides are not strictly limited to plants, and have been screened from fungi and algal sources, suggesting that there are likely a multitude of such metabolites with potential health benefits that are yet to be discovered in nature.
Author contributions
BL and ZL: conceptualization. YN and WL: writing – original draft preparation. BL, ZL, XF, DWX, DW, and HW: revising entire manuscript draft. BL, ZL, and XF: supervising, reviewing and editing final version of article. All authors have read and agreed to the published version of the manuscript.
Funding
The study was supported by Special Item of Regional Collaborative Innovation in Tibet Autonomous Region (QYXTZX-LS2021-01) and Key Research and Development Pro-gram in Tibet Autonomous Region (XZ202201ZY0004N and XZ202301ZY0018N).
Acknowledgments
We gratefully acknowledge Isaac V. Greenhut, ELS for advice with language and editorial assistance in manuscript preparation.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
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Keywords: polysaccharides, gut microbiota, structure, bioactivities, prebiotics
Citation: Niu Y, Liu W, Fan X, Wen D, Wu D, Wang H, Liu Z and Li B (2023) Beyond cellulose: pharmaceutical potential for bioactive plant polysaccharides in treating disease and gut dysbiosis. Front. Microbiol. 14:1183130. doi: 10.3389/fmicb.2023.1183130
Edited by:
Hui Yin Tan, Tunku Abdul Rahman University College, MalaysiaReviewed by:
Phisit Seesuriyachan, Chiang Mai University, ThailandTünde Pusztahelyi, University of Debrecen, Hungary
Copyright © 2023 Niu, Liu, Fan, Wen, Wu, Wang, Liu and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Zhenjiang Liu, emhlbmpsaXVAamx1LmVkdS5jbg==; Bin Li, eHVrZXN1b2xpYmluQDE2My5jb20=
†These authors share first authorship