Jin Zhan
Yulin Li1*
Hongling Yang
Zhiying Ning
Rui Zhang- 1Naiman Desertification Research Station, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, China
- 2College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, China
The plant and soil microbial communities are influenced by variability in environmental conditions (e.g., nitrogen addition); however, it is unclear how long-term nitrogen addition and litter manipulation affect soil microbial communities in a semiarid sandy grassland. Therefore, we simulated the impact of N addition and litter manipulation (litter removal, litter doubling) on plant and soil microbial communities in Horqin grassland, northern China through an experiment from 2014 to 2019. Our results revealed that in the case of non-nitrogen (N0), litter manipulation significantly reduced vegetation coverage (V) (p < 0.05); soil bacterial communities have higher alpha diversity than that of the fungi, and the beta diversity of soil fungi was higher than that of the bacteria; soil microbial alpha diversity was significantly decreased by nitrogen addition (N10) (p < 0.05); N addition and litter manipulation had significantly interactive influences on soil microbial beta diversity, and litter manipulation (C0 and C2) had significantly decreased soil microbial beta diversity (p < 0.05) in the case of nitrogen addition (N10) (p < 0.05). Moreover, bacteria were mostly dominated by the universal phyla Proteobacteria, Actinobacteria, and Acidobacteria, and fungi were only dominated by Ascomycota. Furthermore, the correlation analysis, redundancy analysis (RDA), and variation partitioning analysis indicated that the soil fungi community was more apt to be influenced by plant community diversity. Our results provide evidence that plant and soil microbial community respond differently to the treatments of the 6-year N addition and litter manipulation in a semiarid sandy land.
1. Introduction
Plant and soil microorganisms play important roles in regulating food and timber production, soil carbon (C) sequestration, and nutrient cycling in terrestrial ecosystems (Cui et al., 2020; Delgado-Baquerizo et al., 2020). The plant and soil microbial community is highly vulnerable to environmental changes in the context of global changes, such as increased atmospheric N deposition and land desertification (Liu et al., 2011, 2020; Zuo et al., 2012; Guo et al., 2019). Horqin sandy land is a typical semiarid sandy land in northern China, with relatively high dry N deposition (Lue and Tian, 2007). Due to the effect of strong wind in the process of land desertification, the spatial distribution pattern of litter on the ground surface takes place secondary distribution. Research showed that increased atmospheric N deposition and uneven litter distribution are likely to solely or interactively affect various biotic factors [e.g., plant aboveground biomass (AB), plant productivity, plant functional group composition, soil respiration (Zhao X. et al., 2020), microbial respiration] and abiotic factors (e.g., soil temperature, soil moisture, soil microbial biomass C, and soil microbial biomass N) (Shen et al., 2016; Liu et al., 2017; Gao et al., 2018; Gamadaerji et al., 2020; Zhao X. et al., 2020). Estimating the ecological consequences of N deposition and uneven litter distribution, as well as determining the influence on plant and soil microbial community in a semiarid sandy land are thus urgently needed.
N deposition has been an important component in the global N cycle, and anthropogenic N emission was increased since the industrial revolution (Galloway et al., 2008; Liu et al., 2011). China has become the third largest N deposition area since the late 1980s or early 1990s (Goulding et al., 1998). At the same time, N is the major limiting factor for plant growth in most terrestrial ecosystems (Gamadaerji et al., 2020), and a large number of studies have shown that increased atmospheric N deposition can increase plant community productivity, relieve nutrient limitation of microorganisms, promote activity of microorganisms, influence the bacterial and fungal community compositions, and accelerate the decomposition of litter, but significantly decrease plant species diversity in a community (Clark and Tilman, 2008; Isbell et al., 2013; Yue et al., 2016; Wang et al., 2017; Wang J. Q. et al., 2021). Some studies indicated that N deposition led to a change in plant functional group composition in a semiarid grassland (Gamadaerji et al., 2020).
Litter is one of the important carbon pools in the terrestrial ecosystem, and its decomposition process, as an important nutrient release pathway, connects the aboveground and underground carbon cycle process (Sayer et al., 2011). Global changes (e.g., N deposition), human activities, and changes in land use patterns are significantly affecting terrestrial ecosystem net primary production (NPP) and altering aboveground litter input to soil (LeBauer and Treseder, 2008; Fang et al., 2018). Previous studies showed that the litter quantity and decomposition rate would directly affect the process of nutrient absorption and utilization by plants and soil microorganisms, and further regulated the structure and function of an ecosystem (Wardle et al., 2004; Zhang et al., 2018). Focusing on sandy ecosystems, studies have shown that litter crusts can promote nutrient cycling in sandy ecosystems by mediating the restoration of bacterial taxa, rather than fungi, to enhance soil nutrient availability (Liu X. et al., 2021; Liu Y. et al., 2021). So the responses of soil microbial community to litter alteration in different terrestrial ecosystems has not yet reached a universal conclusion. One study indicated that N deposition affected litter decomposition by affecting soil N availability, litter yield and quality, soil biological factors, and factors in relation to litter decomposition (Yang et al., 2017). It’s well known that N addition promotes plant growth and increases litter production. It is noteworthy that when litter and N addition work collectively, how will it affect the plant and soil microbial community in the semiarid sandy land ecosystem?
Previous studies have shown that increased litter input and N addition significantly increased the community productivity in a semiarid grassland (Gamadaerji et al., 2020), litter addition suppressed the AB responses to N addition under ambient precipitation conditions by affecting soil moisture (Shen et al., 2016), and increased N deposition slightly weakened the inhibition of litter removal on soil respiration (Zhao X. et al., 2020). The biomass of perennial bunch grass (PB) and perennial rhizome grasses (PR) increased significantly with the increment of litter and nitrogen in a semiarid grassland after 6 year observation (Gamadaerji et al., 2020). Study on nitrogen and litter addition showed that litter addition increased AB and belowground net primary productivity (BNPP) in Inner Mongolia grassland after a two-year observation (Shen et al., 2016). In summary, when studying the impact of N addition and litter manipulation on plant and soil microbial communities, most researchers have focused on a single factor [e.g., N addition (Hou et al., 2021)] and have analyzed only one community [e.g., either bacteria or fungi, (van Diepen et al., 2017; Liu et al., 2020)]. But it is unknown how plant and microbial communities respond to N addition and litter manipulation in consecutive years. Thus, in this study, we examined the impact of N addition and litter manipulation (litter removal, litter doubling) on plant and soil microbial communities in a semiarid sandy land in northern China from 2014 to 2019. We hypothesized that (1) N addition can significantly affect plant and soil microorganisms, while litter treatment has interaction with N addition on the plant and soil microbial communities. (2) Fungal communities were more susceptible to N addition and litter treatment than that of bacteria in this semiarid sandy land over these 6 years. (3) The effect of plant community on soil fungal and bacterial community under N addition and litter manipulation was significantly different.
In this work, on the one hand, we can fill in the knowledge gap of the impact of long-term nitrogen addition and litter treatment on soil microbial communities in semi-arid sandy land. And on the other hand, we can improve our understanding of how sandy land ecosystems in semi-arid areas respond to environmental changes. It will provide reference for the follow-up research on the soil-plant-microbe interaction mechanism in semi-arid areas under long-term nitrogen deposition and aboveground litter change treatments.
2. Materials and methods
2.1. Study area
The study was conducted at a semiarid sandy land near Inner Mongolia Naiman Agroecosystem National Observation and Research Station (42°55′N, 120°41′E), in the southwestern part of the Horqin sandy land. The mean annual temperature is between 6.4 and 6.9°C. The mean annual precipitation is between 343 and 451 mm, with > 75% falling in the growing season (May to September). The mean annual wind speed is from 3.5 to 4.5 ms–1, the windy days are between 20 and 60 days, and sandstorms often occur in spring (about 10–15 days). It is mostly northwest wind in winter and spring and southwest in summer. The local geomorphic types are dominated by slowly-fluctuating sandy lands, with fixed dunes, semi-mobile dunes, mobile dunes, and inter-dune lowlands (Zhao et al., 2008). Affected by climate change and human activities, the region has experienced a different degree of desertification (Zuo et al., 2012; Zhao et al., 2014). The zonal soils are sandy chestnut soils with loose structures and vulnerable to wind erosion (Li Y. L. et al., 2016; Zuo et al., 2018). The soil is poor and mostly aeolian soil, and in the 0–30 cm soil layer, the soil nitrogen content was 0.057∼0.199 g.kg–1, the soil bulk density was 1.29∼1.59 g.cm–3 (Mao et al., 2012). In the 0–10 cm soil layer, the soil bulk density was about 1.41 g.cm–3, total nitrogen was about 0.57 g.kg–1, total carbon was about 4.16 g.kg–1 (Lv et al., 2021), and organic carbon was about 1.72 g.kg–1 (Chen et al., 2016). The dominant plant species are Cleistogenes squarrosa (Trin.) Keng, Chenopodium acuminatum Willd, Pennisetum centrasiaticum Tzvel, and Setaria viridis (L.) Beauv.
2.2. Experiment design
The field experiment of N addition and litter manipulation was established in 2014. The experiment was conducted in a randomized block design of six replicate blocks. In each block, we created six 10 m × 10 m plots and the plots were all spaced by 1 m apart to avoid cross effects between treatments. In this study, we only selected the experimental plots treated with nitrogen addition and litter manipulation for observation (Supplementary Figure 1). For the purpose of this study the experimental platform had set up 3 L manipulation levels [litter removal (C0), control (C1) and litter doubled (C2)] and 2 N addition levels [non-nitrogen addition (N0) and nitrogen addition (N10)], and composed of 6 treatments: N0C0, N0C1, N0C2, N10C0, N10C1, N10C2. So every treatment has six replicates. According to a typical practice of N treatment in many scientific simulation experiments of N deposition (Hasselquist et al., 2012; Gao et al., 2018), the experiment of N addition had been conducted at the beginning of May each year in the form of urea [CO(NH2)2] and the amount of N addition was 10 g N m–2 year–1. The urea was dissolved in 10 L purified water and sprayed evenly to each nitrogen addition (N10) plot, with a backpack sprayer. An equal amount of water was applied to the non-nitrogen addition (N0) areas. The experiment of litter manipulation was implemented at the end of November each year including N0 and N10. C0 has removed all litter materials on the soil surface, C1 was in a natural condition and remained intact. C2 has evenly added the litter collected in C0 to the corresponding plot of the same size. The main species of litter collected are Artemisia scoparia Waldst. et Kit., P. centrasiaticum Tzvel., S. viridis (L) Beauv., and C. squarrosa (Trin.) Keng.
2.3. Soil sampling
By the end of the plant growth season in September 2019, we collected 5 subsamples of soil at a depth of 10 cm using a soil corer with a diameter of 2.5 cm in each plot. The 5 samples were thoroughly mixed into a composite sample in the field, and then sieved with a 2 mm mesh to remove any roots or stones. The soil was stored at −80°C for analyzing the bacterial and fungal communities.
2.4. Measurement of plant community
At each block, 1 m × 1 m plots were established for vegetation surveys, and we surveyed plant species richness (S) and vegetation coverage (V) in September 2019. Four general diversity indexes are selected for calculation and analysis of plant diversity: Species richness (S, Equation 1), Shannon–Wiener’s diversity index (H, Equation 2), Pielou’s evenness index (E, Equation 3), and Simpson’s dominance index (λ, Equation 4) to evaluate plant community characteristics (Zhan et al., 2019). The calculation equations are:
where, Pi is the ratio of the number of individuals of the i-th species in the 1 m × 1 m plots to the total number of all species in the sample plot (Zhan et al., 2019; Zhou et al., 2021).
2.5. Measurement of soil microbial community
Total genomic DNA was extracted from 0.4 g of well-mixed soil using the Power Soil DNA Isolation Kit (MoBio Laboratories, Carlsbad, USA) in accordance with the manufacturer’s specifications. Total genomic DNA was subjected to high-throughput sequencing using an IlluminaMiSeq platformat the Novogene Cooperation (Beijing, China). A detailed procedure can be found in the Supplementary information (Supplementary measurement of soil microbial community).
2.6. Statistical analysis
Statistical analyses were performed using SPSS22.0 (USA). We used multi-factor variance analysis to test the significance of the impact of N addition and litter manipulation on plant and soil microbial communities. Then, the effect of different litter manipulation levels on all response variables was tested using one-way ANOVA, and the effect of N addition levels on all response variables was tested by independent sample t-tests. Significant differences were assessed at p < 0.05. We performed non-metric multidimensional scaling (NMDS) analysis of soil microbial communities through the vegan package in R (version 3.6.2) using Bray–Curtis. Furthermore, we performed analysis of similarities (ANOSIM) to test whether the responses of microbial community profiles were significant through the vegan package in R (version 3.6.2). The correlation analysis of plant community diversity with soil microbial alpha diversity and community composition was conducted by OriginPro2021 (USA). The redundancy analysis (RDA) was performed using the “vegan” package in R (version 3.6.2), and p-values of the influence of each plant factor on soil microbial species distribution were calculated by the Envifit function. Variation partitioning analysis (VPA) was performed to determine the relative contributions of vegetation coverage, species richness, and plant diversity indexes (H, E and λ) to shaping the soil fungal and bacterial community composition.
3. Results
3.1. Responses of plant community diversity to N addition and litter manipulation
N addition and litter manipulation had no significantly interactive influences on plant community diversity indexes at the end of 6-year (Supplementary Figure 2). Compared with non-nitrogen (N0), the nitrogen addition (N10), respectively, increased vegetation coverage (V), Pielou’s evenness (E) and Simpson’s dominance (λ) index by 7.16, 16.84, and 5.36% (Supplementary Figures 2A, C, E), and decreased species richness (S) and Shannon–Wiener’s diversity (H) by 15.06 and 2.46% (Supplementary Figures 2B, D). Compared with litter control (C1), litter removal (C0) greatly reduced vegetation coverage (V) and species richness (S) but increased Pielou’s evenness (E) index (Supplementary Figures 2A–C). Furthermore, in the case of non-nitrogen (N0), litter manipulation significantly reduced vegetation coverage (V) (Supplementary Figure 2A, p < 0.05).
3.2. Responses of soil microbial alpha and beta diversity to N addition and litter manipulation
Microbial alpha diversity was used to describe the composition of microbial community for a single habitat or treatment, and beta diversity was used to describe the assembly of microbial communities among different habitats or treatments. In our study, the alpha diversity of fungi was lower than that of bacteria. N addition and litter manipulation had no significantly interactive influences on soil fungal and bacterial alpha diversity at the end of 6-year (Figure 1 and Supplementary Figure 3). Nitrogen addition (N10) significantly reduced fungal alpha diversity (Figure 1 and Supplementary Figure 3, p < 0.05). Similarly, nitrogen addition (N10) significantly decreased bacterial Simpson, chao1, and Coverage estimators based on abundance (ACE) index (Figure 1 and Supplementary Figure 3, p < 0.05). Furthermore, in the case of nitrogen addition (N10), Shannon index was increased by litter doubling (C2), but decreased by litter removal (C0) in fungi (Figure 1A, p < 0.05).
Figure 1. The 6-year of soil fungal and bacterial Shannon diversity index and chao1 richness in N addition and litter manipulation treatments during 2014–2019. Significance levels were presented to show the effect of N addition (N) and altered litter input (C) treatments and their interaction (N × C) on these parameters (nsp > 0.1; #p < 0.1; *p < 0.05; **p < 0.01; ***p < 0.001). The uppercase letters indicate the significant difference between two nitrogen treatments (p < 0.05), different lowercase letters indicate significant difference among three altered litter input treatments in the case of same nitrogen (p < 0.05). (A–D) Represents the α diversity indices of soil fungi and bacteria (Shannon index and Chao1 index), respectively.
The beta diversity of soil fungi was higher than that of bacteria. In fungi, beta diversity was significantly reduced by nitrogen addition (N10) (Figure 2, p < 0.05). In contrary, nitrogen addition (N10) significantly increased soil bacteria beta diversity (Figure 2, p < 0.05). The beta diversity of fungi and bacteria were significantly decreased by litter manipulation (C0 and C2) (Figure 2, p < 0.05). N addition and litter manipulation had significantly interactive influences on soil fungal and bacterial beta diversity at the end of 6-year (Figure 2, p < 0.05). In the case of non-nitrogen addition (N0), the beta diversity of fungi was significantly decreased by litter doubling (C2) (Figure 2, p < 0.05). Furthermore, in the case of nitrogen addition (N10), the beta diversity of fungi and bacteria were significantly decreased by litter manipulation (C0 and C2) (Figure 2, p < 0.05). To further explore the differences in beta diversity, NMDS analysis was carried out. In fungi and bacteria, the Bray–Curtis dissimilarity from the nitrogen addition (N10) and non-nitrogen (N0) treatments was separated along the X- or Y-axis (Figure 3, stress = 0.173, stress = 0.109). Furthermore, the ANOSIM highlighted that the soil fungal and bacterial communities with the nitrogen addition (N10) were substantially different from those of non-nitrogen (N0) treatments (p < 0.05). The clear differences in soil fungal and bacterial communities under N addition and litter manipulation during 2014–2019 were shown in Supplementary Table 1.
Figure 2. The 6-year of soil fungal and bacterial beta diversity in N addition and litter manipulation treatments during 2014–2019. Significance levels were presented to show the effect of N addition (N) and altered litter input (C) treatments and their interaction (N × C) on these parameters (nsp > 0.1; #p < 0.1; *p < 0.05; **p < 0.01; ***p < 0.001). The uppercase letters indicate the significant difference between two nitrogen treatments (p < 0.05), different lowercase letters indicate significant difference among three altered litter input treatments in the case of same nitrogen (p < 0.05).
Figure 3. Non-metric multidimensional scaling (NMDS) analysis of soil fungal and bacterial beta diversity in N addition and litter manipulation treatments during 2014–2019. R and p-values under the non-nitrogen (N0) and nitrogen addition (N10) treatments comes from the ANOSIM analysis.
3.3. Responses of soil microbial community composition to N addition and litter manipulation
The Venn diagrams represent the numbers of specific bacterial and fungal species (represented by OTUs) associated with different treatments (Figure 4). A total of 3,139 soil fungal operational taxonomic unit (OTU) species and 6,217 bacteria were detected in our study. In the case of non-nitrogen addition (N0), a greater number of treatment-specific fungal species were detected in each treatment than bacterial species (Figure 4). In fungi, the core common species dominated the study area and represented 69.25∼85.30%. In addition, compared with non-nitrogen (N0), nitrogen addition (N10) reduced the OTU species from 1,505.67 to 1,300.67 (Figure 4 fungi). In comparison, the core common bacterial species accounted for 86.97∼92.34%, whereas there was little difference between nitrogen addition (N10) and non-nitrogen (N0) (Figure 4 bacteria).
Figure 4. Venn diagram of the soil fungal (left) and bacterial (right) OTU in N addition and litter manipulation treatments during 2014–2019. The numbers within circles represent the specific OTU in that treatment, the core number represents the common OTU present in all treatment.
N addition and litter manipulation had an influence on the relative abundance of many common taxa (top 10%) at the phylum level in soil fungi and bacteria (Supplementary Table 2). Soil fungal communities changed lesser along with different treatments than bacteria. Specifically, the Ascomycota (49.20∼65.63%) was relatively dominant in fungi (Figure 5 fungi), then the Basidiomycota was 2.50∼7.61% and Mortierellomycota was 3.29∼7.96%. In comparison, the bacterial community was getting more diverse among different treatments. The Proteobacteria (26.08∼34.03%), Actinobacteria (27.73∼33.58%), Acidobacteria (10.24∼18.09%), and Firmicutes (3.46∼7.83%) were the most abundant bacterial phyla (Figure 5 bacteria). Meanwhile, the result of the independent sample t-test for N addition treatment showed that nitrogen addition (N10) treatment significantly increased the relative abundance of Ascomycota, but decreased the relative abundance of Chytridiomycota (p < 0.05) in fungi (p < 0.05) (Supplementary Figures 4A, B, p < 0.05). Similarly, nitrogen addition (N10) treatment significantly increased the relative abundance of Proteobacteria, Firmicutes, Bacteroidetes, and Thaumarchaeota (Supplementary Figures 4C, D, p < 0.05), but decreased the relative abundance of Acidobacteria (p < 0.05) in bacteria (Supplementary Figure 5, p < 0.05).
Figure 5. Soil microbial community proportion at phylum level in N addition and litter manipulation treatments during 2014–2019 (mean).
3.4. Relationship between the soil microbial community and plant community diversity under N addition and litter manipulation
The correlation analysis result revealed that Shannon, chao1 and ACE indexes of fungi were positively correlated with species richness (S) (Supplementary Figure 6, p < = 0.05). Similarly, Simpson index of bacteria was positively correlated with species richness (S) (Supplementary Figure 6, p < = 0.05). Furthermore, the Pielou’s evenness (E) decreased significantly with an increasing relative abundance of Mucoromycota in fungi (Supplementary Figure 6 fungi, p < 0.05). Whereas vegetation coverage (V) increased significantly with the increasing relative abundance of Gemmatimonadetes in soil bacteria (Supplementary Figure 6 bacteria, p < 0.05).
The effect of N addition and litter manipulation on soil microbial community was analyzed using RDA (Figure 6). The cumulative percentage variance of the first and second axes was 24.95 and 22.85% for fungi, and 27.31 and 20.45% for bacteria, respectively, indicating that the soil microbial species distribution was significantly affected by the plant community diversity (Figure 6). And the Envifit function results indicated that species richness (S) and vegetation coverage (V) were the most important influential factors to the changes in soil microbial species distribution (p < 0.01). Furthermore, Shannon–Wiener’s diversity (H) and Simpson’s dominance (λ) had significant effect on soil fungal species distribution (p < 0.01), and Pielou’s evenness (E) had a significant effect on soil bacterial species distribution (p < 0.05).
Figure 6. Redundancy analysis (RDA) of plant community diversity and soil microbial species distribution in community under N addition and litter manipulation. Significance levels of each plant community factor were calculated by Envifit function (nsp > 0.1; #p < 0.1; *p < 0.05; **p < 0.01; ***p < 0.001).
A variation partitioning analysis further demonstrated that the soil bacterial and fungal communities were highly explained by vegetation coverage, species richness, and diversity indexes (Figure 7). For fungi, a total of 22.69% of the community variation could be explained by plant variables, and the species richness explained 17.74% of the variation. For bacteria, a total of 12.02% of the community variation could be explained by plant variables, and plant diversity indexes explained 5.98% of the variation (Figure 7).
Figure 7. Variation partitioning analysis (VPA) of the fungal and bacterial community explained by vegetation coverage, species richness and plant’s diversity indexes (H, E, and λ) and their interactions. The value of black circle is the common explanation of the factors at both ends.
4. Discussion
4.1. Effects of N addition and litter manipulation on plant community diversity
In our study, nitrogen addition (N10) had no significant influences on vegetation coverage (V) and plant community diversity, and there are small effects. The reason, on the one hand, N is a key limiting factor in the growth of plants in arid and semiarid regions, and the required nitrogen for plant growth mainly comes from the soil or plant’s nitrogen fixation (Yang et al., 2017). Nitrogen addition will ease the limiting factor, increase the absorption of CO2 in the atmosphere by plants, significantly improve photosynthesis efficiency (Gao Y. et al., 2013), and is conducive to plant growth. So vegetation coverage (V), Pielou’s evenness (E) index, and Simpson’s dominance (λ) were increased in the semiarid sandy land. On the other hand, the addition of nitrogen greatly intensifies interspecific competition in the plant community, tall plants produce photoinhibition to short plants (Li C. B. et al., 2016), at the same time, the increase of plant vegetation coverage (V) under nitrogen addition weakens the surface solar radiation (Kan et al., 2015; Zhao X. X. et al., 2020), which is not conducive to the growth of bottom plants. So nitrogen addition (N10) reduced species richness (S) and Shannon–Wiener’s diversity (H). The plant community did not show significant difference at the end of the 6-year N addition in the semiarid sandy land. The discrepancies could due to that plant diversity responses may also vary depending on the N deposition levels (Lu et al., 2011), different plants with divergent N utilization (Gherardi et al., 2013) and experimental duration (De Schrijver et al., 2011).
Our study presented that litter removal (C0) greatly reduced vegetation coverage (V) and species richness (S). In contrast, litter doubling (C2) slightly increased vegetation coverage (V) and species richness (S) in nitrogen addition (N10), and slightly reduced vegetation coverage (V) and species richness (S) in non-nitrogen (N0). The results addressed part of our first hypothesis: litter manipulation had no significant influence on the plant community. The explanation for the observed differences might be that: First, litter removal (C0) inhibited seed activity of some species or prolonged seed dormancy by increasing soil temperature and reducing soil moisture, thereby significantly inhibiting seed germination (Cuena-Lombrana et al., 2016). Second, litter removal (C0) increased near-surface photosynthetically active radiation, accelerated water loss, reduced photosynthetic rate, and indirectly increased seedling mortality (Bajwa et al., 2017; Zhang, 2019). So litter removal (C0) greatly reduced vegetation coverage (V) and species richness (S). Third, nitrogen addition alleviated the N limitation of plant growth in this area, and litter doubling (C2) slowed evaporation of soil surface moister, promoted the growth of annual species in semiarid areas, and hence increased vegetation coverage (V) and species richness (S). However, in the non- nitrogen addition, the smaller seeds of one or two annual plants stayed in the litter layer (Wang et al., 2013), leading to delay or failure of germination and reducing vegetation coverage (V) and species richness (S).
4.2. Effects of N addition and litter manipulation on soil microbial diversity
Bacteria has higher alpha diversity than fungi in many ecosystems (Anderson et al., 2017; Chen et al., 2020). Our study confirmed this statement in the semiarid sandy land, and fungal alpha and beta diversity were significantly decreased by nitrogen addition (p < 0.05). In fact, fungi typically has lower nutrient requirements than bacteria (Zhou et al., 2017). By contrast, bacteria grow faster and prefer substrates of low C: N ratios. Plants provide soil microbes with C in exchange for other soil nutrients, such as N (Vasar et al., 2017). N availability is increased due to N deposition in soil, plants release lesser amounts of C to soil microbes (Johnson and Thornley, 1987), lesser C could be allocated to belowground parts, resulting in a decrease in the soil C storage in a long term. Consequently, with the addition of nitrogen, when the soil C/N ratio was low, the effect on fungi is greater than bacteria. Our study showed that soil bacterial and fungal alpha and beta diversity index changed to different degrees under nitrogen addition. A explanation for the discrepancies may be the fact that bacteria and fungi are the two major microbial taxa in soils, and they respond differently concerning their morphological traits, utilization strategies, and sensitivities to the environment (Chen et al., 2020).
Litter accumulation can promote the formation of soil organic carbon and affect the community of soil microorganisms (Wang L. Y. et al., 2021). In our study, litter manipulation (C) had no significant influence on soil microbial alpha diversity. This is inconsistent with some studies showing that after the removal or addition of litter in soil, the alpha diversity indexes of soil microbial community have changed significantly (Wang L. Y. et al., 2021). One study indicated that the input of plant litter did not affect the soil organic carbon content (SOC) content in the observation of two temperate forests, even after 11 years of treatment (Holub et al., 2005). And one study indicated that the underground root system may provide a more stable carbon source for the soil than the above-ground litter (Sokol et al., 2019). Therefore, we should consider the influence of underground litter and/or the coupled impacts with the above ground litters on soil microbial community in the later study.
4.3. Effects of N addition and litter manipulation on soil microbial community composition
The microbial OTU taxonomic composition excavates the specific difference along with environmental change. As our Venn diagrams indicated that the core common species accounted for 86.97∼92.34% of the total OTU species in soil bacteria, which were less specific and more adaptable along with environmental change than the fungal species that the core common species represented 69.25∼85.30% of the total soil fungi. In addition, Ascomycota’s proportion was greater than 48% in fungal at the phylum level. Proteobacteria, Actinobacteria, and Acidobacteria were relatively dominant in bacteria. These results confirmed that most soil bacterial taxa occupied a wide range, On the other hand, most soil fungal taxa occupied a narrower range. Therefore, soil bacteria adapted to the environment by changing the proportions of their taxa, while fungi changed their rare taxa in response to environmental change. This was consistent with the results that soil bacterial and fungal communities adapted to the natural aridity gradient in desert grassland ecosystems (Wang S. K. et al., 2021).
Furthermore, nitrogen addition (N10) significantly increased the relative abundance of Ascomycota, Proteobacteria, Firmicutes, Bacteroidetes, and Thaumarchaeota (p < 0.05), but significantly decreased the relative abundance of Acidobacteria (p < 0.05). There are several possible explanations for this result. On the one hand, nutrient enrichment may alter the interactions among microbial species, shifting from symbiosis to competition with increased nutrient supply (Hoek et al., 2016), leading to changes in soil microbial community composition. On the other hand, as the fact that, microbial taxa can have niche preferences, even within the same phylum (Faust and Raes, 2012). For example, our study showed that in semi-arid sandy land, continuous 6 years nitrogen addition was beneficial to Copiotrophic taxa, Proteobacteria’s reproduction, but was not apt to oligotrophic taxa Acidobacteria’s survival.
4.4. Relationship of plant community diversity with soil microbial community under N addition and litter manipulation
Plant species are the main driving factor for microenvironmental change, affecting soil nutrient availability (Schiedung et al., 2017) and ultimately the microbial community (Zuo et al., 2016; Wang et al., 2018). We found that only the species richness (S) was significantly positively associated with some alpha diversity indices of soil microorganisms, and RDA showed that the soil microbial species distribution was significantly affected by the plant community diversity. A variation partitioning analysis further demonstrated that plant variables explained the change in soil fungal and bacterial community by 22.69 and 12.02%. These results indicated that plant community diversity influenced soil fungi more than bacteria. One explanation for this association is that shared environmental factors contribute to relationships between soil microorganisms and plant community composition (Prober et al., 2015). First, plants provide microhabitats as well as organic substrates for soil microorganisms, and such changes in plant community composition lead to changes in both habitats and carbon resources for soil microhabitats (Ramirez et al., 2012), and translating into changes in soil microbial communities. Second, changes in the soil microbial community can affect the plant community. For instance, plant-soil feedback is associated with the processes of soil organic matter decomposition and mineralization, or pathogenic and beneficial interactions (De Deyn and Van der Putten, 2005; Brigham et al., 2022). Third, fungi are often more directly dependent on plant products and mycorrhizal fungi are more dependent on direct symbiotic relationships with plants (Gao C. et al., 2013; Prober et al., 2015). As in this study, soil fungi Shannon, chao1 and ACE indices were significantly positively correlated with species richness (S).
Our results showed that N addition and litter manipulation had no significantly interactive influences on plant and soil microbial community except that soil microbial beta diversity. There are several possible explanations for this result. On the one hand, N addition was dependent on the water condition (Zong et al., 2019). The interannual variability of precipitation in the semi-arid area fluctuates greatly, which will affect the nitrogen use efficiency. On the other hand, as a nutrient provider, litter needs to accumulate in a certain time scale during its decomposition process and its nutrient utilization. Thence to further explore the interaction between N addition and litter manipulation, we need more long-term data accumulation or consideration of factors such as moisture, precipitation, and time in the data analysis process. In this work, we focused on sandy ecosystems in semi-arid regions and filled in the gaps in knowledge about the effects of nitrogen deposition and aboveground litter treatment on soil microbial communities on long-term scales. However, soil microbial communities are mainly regulated by multiple environmental factors, such as climate, topography, soil properties, and vegetation type (Bodelier, 2011; Yang et al., 2018). Nitrogen addition causes soil acidification, if at the end of nitrogen addition, accompanied by a large amount of natural precipitation, the semiarid sandy soil has poor water and fertilizer retention capacity and is prone to leaching, which will affect the utilization of nitrogen by plants and shallow soil microorganisms. So to more rigorously distinguish the effects of abiotic environments on plant communities and soil microbial communities, and the relationship between plant communities and soil microorganisms, it is necessary to conduct experiments that simultaneously manipulate plant communities, soil microorganisms, and related abiotic factors.
5. Conclusion
This study demonstrated that continued N addition and litter manipulation for 6 years had no significant interactive influences on plant and soil microbial community except for soil microbial beta diversity in a semiarid sandy land. Whereas soil microbial alpha and beta diversities were significantly decreased by nitrogen addition (p < 0.05) except for bacterial beta diversity. Furthermore, soil bacterial and fungal communities responded differently, bacterial communities showing higher alpha diversity than fungi, and the beta diversity of soil fungi higher than bacteria. In particular, soil bacteria were dominated by the universal phyla, while fungi were dominated only by the phylum Ascomycota. Results from our study also indicated that most soil bacteria (of the same taxa) occupied a wide range and adapted to the environment by changing the proportions of their taxa, while fungi changed their rare taxa in response to environmental change. Finally, the soil microbial species distribution was significantly affected by the plant community diversity. A variation partitioning analysis further demonstrated that plant variables explained the changes in soil fungal and bacterial communities by 22.69 and 12.02%.
Data availability statement
The datasets presented in this study are deposited in the NCBI repository, accession number: PRJNA944160.
Author contributions
YL, XZ, JZ, HY, and ZN conceived and designed the study. XZ, JZ, HY, and RZ performed the experiments. JZ analyzed the results and drafted the manuscript. All authors had a chance to review the manuscript before submission, contributed to the discussion and interpretation of the data, and approved the submitted version.
Funding
This work was supported by the Key Science and Technology Project of Inner Mongolia Autonomous Region (2021ZD001505 and 2019ZD00704), the National Natural Science Foundation of China (32071845), and Class A Strategic Pilot Science and Technology Project of Chinese Academy of Sciences (XDA23060404).
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Supplementary material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2023.1013570/full#supplementary-material
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Keywords: soil microbial community, plant, nitrogen addition, litter manipulation, Horqin sandy land
Citation: Zhan J, Li Y, Zhao X, Yang H, Ning Z and Zhang R (2023) Effects of nitrogen addition and plant litter manipulation on soil fungal and bacterial communities in a semiarid sandy land. Front. Microbiol. 14:1013570. doi: 10.3389/fmicb.2023.1013570
Received: 07 August 2022; Accepted: 07 March 2023;
Published: 27 March 2023.
Edited by:
Bruno Tilocca, Magna Græcia University, ItalyReviewed by:
Baizhao Ren, Shandong Agricultural University, ChinaYu Liu, Northwest A&F University, China
Copyright © 2023 Zhan, Li, Zhao, Yang, Ning and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Yulin Li, bGl5bEBsemIuYWMuY24=