Recent Advances in Porcine Reproductive and Respiratory Syndrome Virus NADC30-Like Research in China: Molecular Characterization, Pathogenicity, and Control

The name porcine reproductive and respiratory syndrome virus (PRRSV) NADC30-like was first coined in 2015. It originated from the NADC30 strain that was introduced into China by importing breeding pigs and has since undergone mutations or recombination, resulting in variant viruses. Following widespread outbreaks in China in recent years, these NADC30-like strains have presented major health challenges in swine production systems. Outcomes induced by PRRSV NADC30-like infection are highly variable, ranging from inapparent to severe, depending on the recombination between NADC30 and field PRRSV strains prevalent in swine farms. Vaccines and strict biosecurity measures have been explored to fight this disease; however, current PRRSV commercially modified-live virus vaccines (MLVs) have the potential to revert to virulence and only provide limited or no cross-protection efficacy against NADC30-like strains. PRRSVs will remain an ongoing challenge to the swine industry until safe and effective vaccines or antiviral reagents are developed.

Introduction

Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious disease caused by PRRS viruses (PRRSVs), which are small, enveloped, positive single-stranded RNA viruses in the genus Porartevirus, family Arteriviridae, and order Nidovirales (Adams et al., 2017). Currently, PRRSV exists as two distinct virus species, i.e., PRRSV-1 (known as the European genotype, type strain Lelystad) and PRRSV-2 (known as North American genotype, strain VR-2332), with both species sharing ∼60% nucleotide identity at the genomic level and are subjected to frequent mutation and viral recombination events (Nelsen et al., 1999; Adams et al., 2017).

Porcine reproductive and respiratory syndrome virus contains an infectious RNA genome in a proteinaceous nucleocapsid, surrounded by a lipid layer including five or six enveloped structural proteins. The genome of PRRSV contains ∼15 kb nucleotides, with cap structure at the 5′ end and a poly (A) tail at the 3′ end, and contains approximately 11 open reading frames (ORFs) (1a, 1b, 2a, 2b, 3, 4, 5a, 5, 6, 7, and a short transframe ORF) expressed from genomic and subgenomic (sg) mRNAs (sgmRNAs) (Conzelmann et al., 1993). ORF1a and ORF1b are translated as large polypeptides, pp1a and pp1ab, which are then proteolytically processed into the nonstructural proteins (nsp) of PRRSV (nsp1α, nsp1β, nsp2, nsp2TF, and nsp3–nsp12); ORFs 2–7 encode the structural proteins of PRRSV (GP2a, E, GP3–GP5, M, and N) (Meulenberg et al., 1997; Snijder and Meulenberg, 1998; Snijder et al., 2013).

Since the highly pathogenic PRRSV strains were seeded in swine farms in China in 2006 (Li et al., 2007; Tian et al., 2007; Tong et al., 2007), the clinical prevalence of PRRSV has become complicated. To make matters worse, the PRRSV NADC30 strain began to infect Chinese pigs in 2014 (Zhao et al., 2015; Zhou et al., 2015; Li C. et al., 2016; Li Y. et al., 2016). Currently, the recombination characteristics of NADC30 contribute to the emergence of variant NADC30-like viruses, as well as the highly variable clinical symptoms, ranging from inapparent to severe symptoms (Zhao et al., 2015; Zhou et al., 2015; Wang et al., 2018). Herein, we review the current information related to the novel PRRSV strains (NADC30-like) in China in terms of their molecular characterization (genetic diversity) and pathogenicity (virulence), and the questionable efficacy of current vaccines against this disease.

Molecular Characterization (Genetic Diversity of Viruses)

The molecular markers of NADC30 are 131-aa discontinuous deletions in nsp2, including a 111-aa deletion at position 322–432, a 1-aa deletion at position 483, and a 19-aa deletion at position 504–522 corresponding to the NADC30 complete sequence (Brockmeier et al., 2012). All the isolates, called NADC30-like strains, also have the same molecular markers and belong to lineage 1 based on GP5 analysis (Figure 1A) and subgroup 1 based on nsp2 analysis (Figure 1B) (Shi et al., 2010).

FIGURE 1

Figure 1. Phylogenetic tree based on PRRSV ORF5 (A) and nsp2 gene (B) sequences. The phylogenetic tree was constructed using the neighbor-joining method in MEGAX64 with 1,000 bootstrap replicates. Multiple sequence alignments were generated using MUSCLE. NADC30 (GenBank number: JN654459) and 17 references NADC30-like PRRSV strains are indicated by closed red triangles and open red triangles, respectively.

Recombination breakpoints occurring in genes encoding nsps and/or minor structural proteins contribute to the variant pathogenicity of individual isolates, including highly pathogenic, moderate virulent, or mild strains, depending on their other recombination positions with variant local PRRSV strains (Zhao et al., 2015; Zhou et al., 2015; Sun et al., 2016; Wang et al., 2018; Zhang et al., 2019). Currently, there is no reasonable explanation for the random recombination of variant NADC30-like isolates; the postulated reason is the pressure of the complicated PRRSV pool in a swine farm pushes the invading NADC30 strain to survive via a new strategy. Those recombination breakpoints can be found in the 5′ UTR, nsp1-9, nsp12, ORF3, and ORF5-3′ UTR (Guo et al., 2019; Zhang et al., 2019; Zhao et al., 2020).

Outcomes Produced by Porcine Reproductive and Respiratory Syndrome Virus NADC30-Like Infection Are Highly Variable

All the NADC30-like isolates reported from swine farm outbreaks of PRRS are characterized by abortions and stillbirth in pregnant sows, as well as high fever, anorexia, red discoloration of the body, blue ears, and respiratory disorders in piglets. The impact of NADC30-like infection contributes to the swine farm referring to its virulence, as well as the age and immune status of the infected pigs, and the presence of concurrent infections. Pig infection experiments in the laboratory may not replicate the outcomes of clinical cases, but can be used to evaluate the pathogenicity of an isolate.

The pathogenicity of NADC30-like strains was first investigated using JL580 (accession no. KR706343), which acquired increased genetic diversity by recombining with local HP-PRRSV 09HEN1 in China at six different sites in its genome (Zhao et al., 2015; Sun et al., 2016). Six-week-old PRRSV-free piglets were inoculated intramuscularly (1 ml) and intranasally (2 ml) with JL580-F2 at 3 × 10^4.0 TCID50 in 3 ml of Dulbecco’s modified Eagle’s medium (DMEM) per pig. These piglets were reported to develop obvious clinical symptoms from 3 days post-inoculation (DPI), e.g., higher fever, cough, anorexia, and red discoloration of the body and ears, accompanied by severe lung lesions with pulmonary consolidation, and interstitial pneumonia (Zhao et al., 2015). The outcomes induced by NADC30-like JL580 are much more severe than those of NADC30 isolates (e.g., HNjz15, CHsx1401, SC-d, SD-A19) (Brockmeier et al., 2012; Sun et al., 2016; Zhou et al., 2017; Wang et al., 2018) and CH-1a (Xue et al., 2004), and are similar to those of the HP-PRRSV strains that appeared in China from 2006 (Li et al., 2007; Tian et al., 2007; Tong et al., 2007).

Similarly, the pathogenicity of another NADC30-like isolate, HNjz15 (accession no. KT945017), with no recombination with other PRRSV strains, as compared with the pathogenicity with highly pathogenic PRRSV JAX1 strain (accession no. EF112445) (Sun et al., 2016). Six-week-old PRRSV-free pigs were inoculated intranasally with 2 ml (5 × 10⁴ TCID₅₀/ml) of HNjz15 or JXA1. Piglets infected with one of the two PRRSV isolates developed typical PRRS symptoms, e.g., high fever and respiratory disorders, and the difference was that JXA1-infected piglets developed more severe clinical manifestations than the HNjz15-infected piglets (Sun et al., 2016). Analysis of the data from HNjz15- and JL580-infected piglets demonstrated that HNjz15 is less pathogenic than the JXA1 and JL580 PRRSV strains, even though those experiments were not performed at the same time.

To further clarify their pathogenic characterization, three isolates, including non-recombinant NADC30-like PRRSV (SD-A19), recombinant NADC30-like PRRSV (SC-d), and highly pathogenic PRRSV (HuN4), were used to inoculate 5-week-old SPF piglets intranasally (3 ml, 1 × 10⁵ TCID₅₀/ml), respectively. The results showed HuN4 infection induced most severe PRRS disease, including rectal temperature, thymic atrophy, and interstitial pneumonia, and the SC-d isolate infection induced milder PRRS disease than that of HuN4, but more severe than that of the SD-A19 isolate (Wang et al., 2018).

Based on cumulative data, recombination is responsible for the pathogenicity variance and genetic diversity of NADC30-like PRRSVs in China, and the pathogenicity tends to be intermediate between those of the parental strains. However, we cannot find the key factors that determine viral pathogenicity.

Lesions of Central Immune Organs

Bone marrow and the thymus are the primary lymphoid organs of the mammalian immune system, which provide suitable sites for antigen-independent B- and T-lymphocyte differentiation from stem cells. Therefore, they play important roles in humoral or cell-mediated immunity, respectively. PRRSV-induced central immune organ lesions have become a concern since severe thymic atrophy induced by highly pathogenic PRRSV infection was first reported (Wang et al., 2011). Since then, different PRRSV-1 and PRRSV-2 isolates with variable pathogenicity have been evaluated for their contribution to bone marrow and thymus lesions (Guo et al., 2013; Amarilla et al., 2016, 2017; Wang et al., 2016, 2018). PRRSV infection of susceptible CD14⁺ cells incapacitates their ability to act against microbial infection and antigen recognition, processing, and presentation to T and B cells; and PRRSV infection induced apoptosis in precursor cells, as well as CD4⁺CD8⁺ thymocytes directly by infection or indirectly via a bystander effect (He et al., 2012; Li et al., 2014; Wang et al., 2016). The cumulative effect leads to immunomodulation, and impairs the host’s ability to resist and/or eliminate secondary infectious agents (Wang et al., 2019, 2020).

Porcine reproductive and respiratory syndrome virus NADC30(-like) infection also induces thymic atrophy. The average ratio of thymus/body weight (g/kg) of piglets induced by recombinant NADC30-like PRRSV SC-d isolate infection was lower than that of piglets induced by non-recombinant NADC30-like PRRSV SD-A19 isolate infection, but was significantly higher than that of piglets induced by the highly pathogenic PRRSV HuN4 strain (Wang et al., 2018). The further data are sparse since the new PRRSV isolates were only reported from 2015 in China. The thymic atrophy induced by PRRSV NADC30 (-like) isolate infection suggested that their infection can cause immunomodulation by destroying the function of central immune organs. Herein, we remind investigators of the concerns regarding thymic atrophy and the mechanism by which PRRS induces these lesions because the mammalian thymus plays important roles in cell-mediated immunity.

Current Commercial Modified Live Vaccines Against Porcine Reproductive and Respiratory Syndrome Virus NADC30-Like Isolates

Currently, different kinds of commercial PRRSV modified-live virus vaccines (MLVs) have been used widely in Chinese swine farms, and these MLVs are effective in homologous PRRSV strain challenges (Tian et al., 2009; Leng et al., 2012; Yu et al., 2015). However, NADC30-like strains inducing PRRS still cause outbreaks in vaccinated pigs, which indicates the inefficiency of current commercial PRRSV vaccines. Some reports offered evidence that current commercial PRRSV MLVs provide cross-, limited, or no protection against NADC30-like PRRSV infection, and these vaccines include VR-2332 (Boehringer-Ingelheim, Mannheim, Germany), JXA1-P80 (Pulike Biological Engineering Co. Ltd., Luoyang, China), HuN4-F112 (Harbin Weike Biotechnology Development Company, Harbin, China), GDr180 (Guangdong Yongshun Biological Pharmaceutical Co. Ltd. Guangdong, China), and TJM-F92 [Qingdao Yibang Biological Engineering Co. Ltd or Sinovet (Beijing) Biotechnology Co. Ltd. or Zoetis] as well as an attenuated low pathogenic PRRSV HB-1/3.9-P40 vaccine that was not commercialized. These commercial vaccines include classical MLVs and HP-PRRSV MLV vaccines used in swine farms in China. The inoculated PRRSV isolates include NADC30-like HNjz15 (accession number KT945017, virulent to pigs, but is less pathogenic than the JXA1 and JL580 PRRSV strains) (Sun et al., 2016), CHsx1401 (GenBank accession no. KP861625, a moderately virulent virus for piglets) (Zhou et al., 2017), HN201605 (with lower pathogenicity than HP-PRRSV strain) (Zhang et al., 2018), and v2016/ZJ/09-03 (a virulent PRRSV) (Chai et al., 2020). The cumulative results demonstrated that the pathogenicity of NADC30-like strains is complicated, and suitable PRRSV MLVs for swine farms remain to be evaluated. However, following reports of more NADC30-like strains, it is likely that PRRSV MLVs will have limited cross-protection efficacy to some NADC30-like strains (Table 1).

TABLE 1

Table 1. The efficacy of commercial PRRSV MLVs to NADC30-like isolates.

Other Attempted Strategies to Control Porcine Reproductive and Respiratory Syndrome in Swine Herds

This section describes attempted strategies to control the PRRS in swine herds in China.

Tylvalosin Attenuated Porcine Reproductive and Respiratory Syndrome Virus-Induced Acute Lung Injury in Piglets

Tylvalosin is a member of third-generation macrolides, which has broad-spectrum activity and can exert a variety of pharmacological effects. It is often used to control the Mycoplasma hyopneumoniae infection in swine farms, and the premix formulation (Aivlosin 42.5 mg/g premix for medicated feedstuff) and oral powder formulation (Aivlosin 42.5 mg/g oral powder for pigs) are licensed in the EU for the treatment and metaphylaxis of EP (CVMP European Public Assessment Report [EPAR], 2013; Lopez Rodriguez et al., 2020). The Committee for Medicinal Products for Veterinary Use (CVMP) European public assessment report (EPAR) for Aivlosin is available online.¹ In recent years, tylvalosin has been advertised because of its anti-PRRSV activities, especially for existing PRRSV NADC30-like or HP-PRRSV-like strains. In China, tylvalosin is used widely to control PRRS in many swine farms. An animal study reported that piglets received feed containing 75 ppm tylvalosin for 28 days, following challenge with PRRSV Hn isolate intranasally (2 ml, 1 × 10^4.5 TCID₅₀/ml), and then continued to receive tylvalosin medicated feed or not for another 21 days. The outcomes showed that tylvalosin attenuated PRRSV-induced clinical disease and improved growth, and similar low-intensity clinical signs were observed in these piglets compared with the piglets infected with the PRRSV Hn isolate directly (Zhao et al., 2014). Mechanistically, it was postulated that tylvalosin inhibited PRRSV-induced NF-κB activation, as well as the production of inflammatory cytokines, such as IL-6, IL-8, and TNF-α (Zhao et al., 2014).

Bioactive Compounds From Traditional Chinese Medicines Exhibit Anti-Porcine Reproductive and Respiratory Syndrome Virus Activities

Bioactive compounds from TCMs exhibit features of PRRSV replication inhibition; therefore, many crude TCM herbal extracts have emerged as effective alternatives in the control of PRRS, as premixes for medicated feedstuff or administration via intramuscular injection. The mechanisms by which bioactive compounds of TCMs inhibit PRRSV replication in vitro have been reviewed in detail (Bello-Onaghise et al., 2020), and include blocking PRRSV attachment and entry into cells, exerting activity against different stages of the PRRSV life cycle (i.e., interfering with viral RNA replication, viral particle assembly, and particle release), or acting as immunomodulators through cytokine regulation.

Since NADC30-like strains and HP-PRRSV-like strains appeared in swine farms, the commercially available PRRSV MLVs have provided limited protection or no protection against these genetically diverse isolates. Some bioactive compounds from TCMs remain popular in swine farms in China in different forms, including medicated feedstuff or oral or injected formulations.

Strict Biosecurity Measures

In China, a set of strict biosecurity measures were carried out in response to the African swine fever virus (ASFV), a highly contagious virus that is a major threat to domestic pigs and wild boars, which appeared in China in 2018. Since there was no effective vaccine or medicine that could be used to control the disease, biosecurity measure was the only way to prevent virus transmission. Risks, including people, pigs, vehicles and equipment, feed and water, as well as pests and air, were treated sterilely as soon as possible to keep this swine pathogen out of swine farms. The outcome was that the swine populations seemed to have remained healthy for a long time. Thus, many swine farmers have given their herds PRRSV vaccination. However, PRRSVs exist in most swine farms in China, and free use of PRRSV vaccination has led to HP-PRRSV-like or NADC30-like strains re-emerging in seemingly healthy populations since the end of 2020.

Inactivated Porcine Reproductive and Respiratory Syndrome Virus Vaccines

Different adjuvants (cytokines, chemical reagents, and bacterial products; detailed in Charerntantanakul, 2009), different administration routes (intramuscular or into the skin using dissolving microneedle patches), and different inactivation manners [binary ethyleneimine (BEI)-inactivated, beta-propiolactone (BPL)-inactivated, or ultraviolet (UV)-inactivated] have been tested to obtain inactivated PRRSV vaccine candidates to provide a better protective efficacy against clinical PRRSV isolate infection in piglets. Although some attempts could induce humoral immune responses, no successful cases have been reported that provided satisfactory protection efficacy to relieve clinical symptoms and reduce viremia and the pathological changes in the lung and other organs (Nilubol et al., 2004; Tabynov et al., 2016; Vreman et al., 2019, 2021).

There have been no reports about the efficacy of PRRSV-inactivated vaccines against NADC30-like strains in swine farms of China. However, following the appearance of NADC30-like strains in swine farms, sows have continued to be inoculated with the only commercial PRRSV-inactivated vaccine (CH-1a strain) in China. Currently, although the protective mechanism of the inactivated vaccine is unclear, it provides good protection for the gilts in PRRSV-affected swine farms.

Implications and Conclusion

As a “mystery swine disease” that first appeared in swine farms (Pol et al., 1991; Stevenson et al., 1993; Wensvoort, 1993; Wills et al., 1997), PRRS has existed for more than 25 years. During this period, researchers have been working to determine the pathogenesis, immune mechanisms, and efficient control measures of PRRS, and have achieved milestones in clarifying the mechanism of immune responses, immunosuppression, and antiviral innate immunity evasion, as well as the development of MLV vaccines to relieve clinical symptoms, and reduce viremia and the pathological changes in the lung and other organs (for detailed information, see Lunney et al., 2016; Du et al., 2017; Wang et al., 2019, 2020). However, because of the recombination and variation characteristics, PRRSVs are still active in swine farms and have evolved into many variant strains, which have inflicted major losses on swine productivity.

In mainland China, since PRRS was first reported in 1996 (Guo et al., 1996), the virus has experienced two main variations. HP-PRRSV was the product of the first variation in May 2006, which represented a great challenge for the development of the pig industry in the following ∼10 years (Li et al., 2007; Tian et al., 2007; Tong et al., 2007). The second variation began in 2014 because of the invasion of the PRRSV NADC30 strain and recombination with the field PRRSV strains that were prevalent in the Chinese swine farms, causing various clinical outcomes from inapparent symptoms to severe symptoms, and representing new challenges for farms (Zhao et al., 2015; Zhou et al., 2015; Sun et al., 2016; Wang et al., 2018; Zhang et al., 2019). Although PRRS was placed under limited control in most swine farms, depending on the commercial MLV vaccines available before 2014, the invasion of PRRSV NADC30 and NADC30-like strain from 2014 onward broke the vulnerable PRRSV control balance. Routine and reliable control of PRRSV will require long-term exploration, and the recombination characteristics of PRRSV contribute to the emergence of variant virus strains, meaning their effects on swine farms must be monitored in the future.

Author Contributions

HS and YY designed the structure and concept of the review. All authors wrote the article, contributed to the article, and approved the final version of the article.

Funding

This work was supported by grants from the National Natural Science Foundation of China (grant number 32072870), the Shandong Province Natural Science Foundation (grant number ZR2021MC008), the State Key Laboratory of Veterinary Biotechnology Foundation (grant number SKLVBF202115), and the Ph.D. Foundation of Qingdao Agricultural University, China (grant number 6631120019).

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s Note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Footnotes

1. ^ https://www.ema.europa.eu/en/documents/product-information/aivlosin-epar-product-information_en.pdf

References

Adams, M. J., Lefkowitz, E. J., King, A. M. Q., Harrach, B., Harrison, R. L., Knowles, N. J., et al. (2017). Changes to taxonomy and the international code of virus classification and nomenclature ratified by the international committee on taxonomy of viruses (2017). Arch. Virol. 162, 2505–2538. doi: 10.1007/s00705-017-3358-5

PubMed Abstract | CrossRef Full Text | Google Scholar

Amarilla, S. P., Gomez-Laguna, J., Carrasco, L., Rodriguez-Gomez, I. M., Caridad, Y. O. J. M., Graham, S. P., et al. (2017). Porcine reproductive and respiratory syndrome type 1 viruses induce hypoplasia of erythroid cells and myeloid cell hyperplasia in the bone marrow of experimentally infected piglets independently of the viral load and virulence. Vet. Microbiol. 201, 126–135. doi: 10.1016/j.vetmic.2016.12.040

PubMed Abstract | CrossRef Full Text | Google Scholar

Amarilla, S. P., Gomez-Laguna, J., Carrasco, L., Rodriguez-Gomez, I. M., Caridad, Y. O. J. M., Graham, S. P., et al. (2016). Thymic depletion of lymphocytes is associated with the virulence of PRRSV-1 strains. Vet. Microbiol. 188, 47–58. doi: 10.1016/j.vetmic.2016.04.005

PubMed Abstract | CrossRef Full Text | Google Scholar

Bai, X., Wang, Y., Xu, X., Sun, Z., Xiao, Y., Ji, G., et al. (2016). Commercial vaccines provide limited protection to NADC30-like PRRSV infection. Vaccine 34, 5540–5545. doi: 10.1016/j.vaccine.2016.09.048

PubMed Abstract | CrossRef Full Text | Google Scholar

Bello-Onaghise, G., Wang, G., Han, X., Nsabimana, E., Cui, W., Yu, F., et al. (2020). Antiviral strategies of chinese herbal medicine against PRRSV infection. Front. Microbiol. 11:1756. doi: 10.3389/fmicb.2020.01756

PubMed Abstract | CrossRef Full Text | Google Scholar

Brockmeier, S. L., Loving, C. L., Vorwald, A. C., Kehrli, M. E. Jr., Baker, R. B., Nicholson, T. L., et al. (2012). Genomic sequence and virulence comparison of four Type 2 porcine reproductive and respiratory syndrome virus strains. Virus Res. 169, 212–221. doi: 10.1016/j.virusres.2012.07.030

PubMed Abstract | CrossRef Full Text | Google Scholar

Chai, W., Liu, Z., Sun, Z., Su, L., Zhang, C., and Huang, L. (2020). Efficacy of two porcine reproductive and respiratory syndrome (PRRS) modified-live virus (MLV) vaccines against heterologous NADC30-like PRRS virus challenge. Vet. Microbiol. 248:108805. doi: 10.1016/j.vetmic.2020.108805

PubMed Abstract | CrossRef Full Text | Google Scholar

Charerntantanakul, W. (2009). Adjuvants for porcine reproductive and respiratory syndrome virus vaccines. Vet. Immunol. Immunopathol. 129, 1–13. doi: 10.1016/j.vetimm.2008.12.018

PubMed Abstract | CrossRef Full Text | Google Scholar

Conzelmann, K. K., Visser, N., Van Woensel, P., and Thiel, H. J. (1993). Molecular characterization of porcine reproductive and respiratory syndrome virus, a member of the arterivirus group. Virology 193, 329–339. doi: 10.1006/viro.1993.1129

PubMed Abstract | CrossRef Full Text | Google Scholar

CVMP European Public Assessment Report [EPAR]. (2013). For Aivlosin. Available online at: https://www.ema.europa.eu/en/documents/variation-report/aivlosin-v-c-83-x-0051-epar-assessment-report-extension_en.pdf (accessed 10 October, 2013).

Google Scholar

Du, T., Nan, Y., Xiao, S., Zhao, Q., and Zhou, E. M. (2017). Antiviral strategies against PRRSV Infection. Trends Microbiol. 25, 968–979. doi: 10.1016/j.tim.2017.06.001

PubMed Abstract | CrossRef Full Text | Google Scholar

Guo, B. Q., Chen, Z. S., Liu, X. W., and Cui, Y. Z. (1996). Isolation and identification of porcine reproductive and respiratory syndrome (PRRS) virus. Chin. J. Anim. Poultry Infect. Dis. 25, 1–5.

Google Scholar

Guo, B., Lager, K. M., Henningson, J. N., Miller, L. C., Schlink, S. N., Kappes, M. A., et al. (2013). Experimental infection of United States swine with a Chinese highly pathogenic strain of porcine reproductive and respiratory syndrome virus. Virology 435, 372–384. doi: 10.1016/j.virol.2012.09.013

PubMed Abstract | CrossRef Full Text | Google Scholar

Guo, Z., Chen, X. X., Li, X., Qiao, S., Deng, R., and Zhang, G. (2019). Prevalence and genetic characteristics of porcine reproductive and respiratory syndrome virus in central China during 2016-2017: NADC30-like PRRSVs are predominant. Microb. Pathog. 135:103657. doi: 10.1016/j.micpath.2019.103657

PubMed Abstract | CrossRef Full Text | Google Scholar

He, Y., Wang, G., Liu, Y., Shi, W., Han, Z., Wu, J., et al. (2012). Characterization of thymus atrophy in piglets infected with highly pathogenic porcine reproductive and respiratory syndrome virus. Vet. Microbiol. 160, 455–462. doi: 10.1016/j.vetmic.2012.05.040

PubMed Abstract | CrossRef Full Text | Google Scholar

Leng, X., Li, Z., Xia, M., He, Y., and Wu, H. (2012). Evaluation of the efficacy of an attenuated live vaccine against highly pathogenic porcine reproductive and respiratory syndrome virus in young pigs. Clin. Vaccine Immunol. 19, 1199–1206. doi: 10.1128/CVI.05646-11

PubMed Abstract | CrossRef Full Text | Google Scholar

Li, C., Zhuang, J., Wang, J., Han, L., Sun, Z., Xiao, Y., et al. (2016). Outbreak Investigation of NADC30-Like PRRSV in South-East China. Transbound. Emerg. Dis. 63, 474–479. doi: 10.1111/tbed.12530

PubMed Abstract | CrossRef Full Text | Google Scholar

Li, Y., Ji, G., Wang, J., Tan, F., Zhuang, J., Li, X., et al. (2016). Complete genome sequence of an NADC30-like porcine reproductive and respiratory syndrome virus characterized by recombination with other strains. Genome Announc 4, e00330–e00416. doi: 10.1128/genomeA.00330-16

PubMed Abstract | CrossRef Full Text | Google Scholar

Li, Y., Wang, G., Liu, Y., Tu, Y., He, Y., Wang, Z., et al. (2014). Identification of apoptotic cells in the thymus of piglets infected with highly pathogenic porcine reproductive and respiratory syndrome virus. Virus Res. 189, 29–33. doi: 10.1016/j.virusres.2014.04.011

PubMed Abstract | CrossRef Full Text | Google Scholar

Li, Y., Wang, X., Bo, K., Tang, B., Yang, B., Jiang, W., et al. (2007). Emergence of a highly pathogenic porcine reproductive and respiratory syndrome virus in the Mid-Eastern region of China. Vet. J. 174, 577–584. doi: 10.1016/j.tvjl.2007.07.032

PubMed Abstract | CrossRef Full Text | Google Scholar

Lopez Rodriguez, A., Berge, A. C., Ramage, C., Saltzman, R., Domangue, R. J., Gnozzio, M. J., et al. (2020). Evaluation of the clinical efficacy of a water soluble formulation of tylvalosin in the control of enzootic pneumonia associated with Mycoplasma hyopneumoniae and Pasteurella multocida in pigs. Porcine Health Manag. 6:39. doi: 10.1186/s40813-020-00177-9

PubMed Abstract | CrossRef Full Text | Google Scholar

Lunney, J. K., Fang, Y., Ladinig, A., Chen, N., Li, Y., Rowland, B., et al. (2016). Porcine reproductive and respiratory syndrome virus (PRRSV): pathogenesis and interaction with the immune system. Annu. Rev. Anim. Biosci. 4, 129–154. doi: 10.1146/annurev-animal-022114-111025

PubMed Abstract | CrossRef Full Text | Google Scholar

Meulenberg, J. J., Petersen den Besten, A., de Kluyver, E., van Nieuwstadt, A., Wensvoort, G., and Moormann, R. J. (1997). Molecular characterization of Lelystad virus. Vet. Microbiol. 55, 197–202. doi: 10.1016/s0378-1135(96)01335-1

PubMed Abstract | CrossRef Full Text | Google Scholar

Nelsen, C. J., Murtaugh, M. P., and Faaberg, K. S. (1999). Porcine reproductive and respiratory syndrome virus comparison: divergent evolution on two continents. J. Virol. 73, 270–280. doi: 10.1128/JVI.73.1.270-280.1999

PubMed Abstract | CrossRef Full Text | Google Scholar

Nilubol, D., Platt, K. B., Halbur, P. G., Torremorell, M., and Harris, D. L. (2004). The effect of a killed porcine reproductive and respiratory syndrome virus (PRRSV) vaccine treatment on virus shedding in previously PRRSV infected pigs. Vet. Microbiol. 102, 11–18. doi: 10.1016/j.vetmic.2004.05.006

PubMed Abstract | CrossRef Full Text | Google Scholar

Pol, J. M., van Dijk, J. E., Wensvoort, G., and Terpstra, C. (1991). Pathological, ultrastructural, and immunohistochemical changes caused by Lelystad virus in experimentally induced infections of mystery swine disease (synonym: porcine epidemic abortion and respiratory syndrome (PEARS)). Vet. Q. 13, 137–143. doi: 10.1080/01652176.1991.9694298

PubMed Abstract | CrossRef Full Text | Google Scholar

Shi, M., Lam, T. T., Hon, C. C., Murtaugh, M. P., Davies, P. R., Hui, R. K., et al. (2010). Phylogeny-based evolutionary, demographical, and geographical dissection of North American type 2 porcine reproductive and respiratory syndrome viruses. J. Virol. 84, 8700–8711. doi: 10.1128/JVI.02551-09

PubMed Abstract | CrossRef Full Text | Google Scholar

Snijder, E. J., and Meulenberg, J. J. (1998). The molecular biology of arteriviruses. J. Gen. Virol. 79(Pt 5), 961–979. doi: 10.1099/0022-1317-79-5-961

PubMed Abstract | CrossRef Full Text | Google Scholar

Snijder, E. J., Kikkert, M., and Fang, Y. (2013). Arterivirus molecular biology and pathogenesis. J. Gen. Virol. 94, 2141–2163. doi: 10.1099/vir.0.056341-0

PubMed Abstract | CrossRef Full Text | Google Scholar

Stevenson, G. W., Van Alstine, W. G., Kanitz, C. L., and Keffaber, K. K. (1993). Endemic porcine reproductive and respiratory syndrome virus infection of nursery pigs in two swine herds without current reproductive failure. J. Vet. Diagn. Invest. 5, 432–434. doi: 10.1177/104063879300500322

PubMed Abstract | CrossRef Full Text | Google Scholar

Sun, Z., Wang, J., Bai, X., Ji, G., Yan, H., Li, Y., et al. (2016). Pathogenicity comparison between highly pathogenic and NADC30-like porcine reproductive and respiratory syndrome virus. Arch. Virol. 161, 2257–2261. doi: 10.1007/s00705-016-2883-y

PubMed Abstract | CrossRef Full Text | Google Scholar

Tabynov, K., Sansyzbay, A., Tulemissova, Z., Dhakal, S., Samoltyrova, A., Renukaradhya, G. J., et al. (2016). Inactivated porcine reproductive and respiratory syndrome virus vaccine adjuvanted with montanide Gel 01 ST elicits virus-specific cross-protective inter-genotypic response in piglets. Vet. Microbiol. 192, 81–89. doi: 10.1016/j.vetmic.2016.06.014

PubMed Abstract | CrossRef Full Text | Google Scholar

Tian, K., Yu, X., Zhao, T., Feng, Y., Cao, Z., Wang, C., et al. (2007). Emergence of fatal PRRSV variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark. PLoS One 2:e526. doi: 10.1371/journal.pone.0000526

PubMed Abstract | CrossRef Full Text | Google Scholar

Tian, Z. J., An, T. Q., Zhou, Y. J., Peng, J. M., Hu, S. P., Wei, T. C., et al. (2009). An attenuated live vaccine based on highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) protects piglets against HP-PRRS. Vet. Microbiol. 138, 34–40. doi: 10.1016/j.vetmic.2009.03.003

PubMed Abstract | CrossRef Full Text | Google Scholar

Tong, G. Z., Zhou, Y. J., Hao, X. F., Tian, Z. J., An, T. Q., and Qiu, H. J. (2007). Highly pathogenic porcine reproductive and respiratory syndrome, China. Emerg. Infect. Dis. 13, 1434–1436. doi: 10.3201/eid1309.070399

PubMed Abstract | CrossRef Full Text | Google Scholar

Vreman, S., McCaffrey, J., Popma-de Graaf, D. J., Nauwynck, H., Savelkoul, H. F. J., Moore, A., et al. (2019). Toll-like receptor agonists as adjuvants for inactivated porcine reproductive and respiratory syndrome virus (PRRSV) vaccine. Vet. Immunol. Immunopathol. 212, 27–37. doi: 10.1016/j.vetimm.2019.04.008

PubMed Abstract | CrossRef Full Text | Google Scholar

Vreman, S., Stockhofe-Zurwieden, N., Popma-de Graaf, D. J., Savelkoul, H. F. J., Barnier-Quer, C., Collin, N., et al. (2021). Immune responses induced by inactivated porcine reproductive and respiratory syndrome virus (PRRSV) vaccine in neonatal pigs using different adjuvants. Vet. Immunol. Immunopathol. 232:110170. doi: 10.1016/j.vetimm.2020.110170

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, G., Li, L., Yu, Y., Tu, Y., Tong, J., Zhang, C., et al. (2016). Highly pathogenic porcine reproductive and respiratory syndrome virus infection and induction of apoptosis in bone marrow cells of infected piglets. J. Gen. Virol. 97, 1356–1361. doi: 10.1099/jgv.0.000454

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, G., Song, T., Yu, Y., Liu, Y., Shi, W., Wang, S., et al. (2011). Immune responses in piglets infected with highly pathogenic porcine reproductive and respiratory syndrome virus. Vet. Immunol. Immunopathol. 142, 170–178. doi: 10.1016/j.vetimm.2011.05.004

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, G., Yu, Y., Cai, X., Zhou, E. M., and Zimmerman, J. J. (2020). Effects of PRRSV infection on the porcine thymus. Trends Microbiol. 28, 212–223. doi: 10.1016/j.tim.2019.10.009

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, G., Yu, Y., He, X., Wang, M., Cai, X., and Zimmerman, J. J. (2019). Porcine reproductive and respiratory syndrome virus infection of bone marrow: Lesions and pathogenesis. Virus Res. 265, 20–29. doi: 10.1016/j.virusres.2019.02.019

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, H. M., Liu, Y. G., Tang, Y. D., Liu, T. X., Zheng, L. L., Wang, T. Y., et al. (2018). A natural recombinant PRRSV between HP-PRRSV JXA1-like and NADC30-like strains. Transbound. Emerg. Dis. 65, 1078–1086. doi: 10.1111/tbed.12852

PubMed Abstract | CrossRef Full Text | Google Scholar

Wensvoort, G. (1993). Lelystad virus and the porcine epidemic abortion and respiratory syndrome. Vet. Res. 24, 117–124.

Google Scholar

Wills, R. W., Zimmerman, J. J., Yoon, K. J., Swenson, S. L., McGinley, M. J., Hill, H. T., et al. (1997). Porcine reproductive and respiratory syndrome virus: a persistent infection. Vet. Microbiol. 55, 231–240.

Google Scholar

Xue, Q., Zhao, Y. G., Zhou, Y. J., Qiu, H. J., Wang, Y. F., Wu, D. L., et al. (2004). Immune responses of swine following DNA immunization with plasmids encoding porcine reproductive and respiratory syndrome virus ORFs 5 and 7, and porcine IL-2 and IFNgamma. Vet. Immunol. Immunopathol. 102, 291–298. doi: 10.1016/j.vetimm.2004.09.021

PubMed Abstract | CrossRef Full Text | Google Scholar

Yu, X., Zhou, Z., Cao, Z., Wu, J., Zhang, Z., Xu, B., et al. (2015). Assessment of the safety and efficacy of an attenuated live vaccine based on highly pathogenic porcine reproductive and respiratory syndrome virus. Clin. Vaccine Immunol. 22, 493–502. doi: 10.1128/CVI.00722-14

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhang, H., Leng, C., Ding, Y., Zhai, H., Li, Z., Xiang, L., et al. (2019). Characterization of newly emerged NADC30-like strains of porcine reproductive and respiratory syndrome virus in China. Arch. Virol. 164, 401–411. doi: 10.1007/s00705-018-4080-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhang, H., Xia, M., Wang, W., Ju, D., Cao, L., Wu, B., et al. (2018). An attenuated highly pathogenic chinese PRRS viral vaccine confers cross protection to pigs against challenge with the emerging PRRSV NADC30-Like strain. Virol. Sin. 33, 153–161. doi: 10.1007/s12250-018-0027-0

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhao, J., Zhu, L., Huang, J., Yang, Z., Xu, L., Gu, S., et al. (2020). Genetic characterization of a novel recombined porcine reproductive and respiratory syndrome virus 2 among Nadc30-like, Jxa1-like and TJ-like strains. Vet. Med. Sci. 7, 697–704. doi: 10.1002/vms3.402

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhao, K., Ye, C., Chang, X. B., Jiang, C. G., Wang, S. J., Cai, X. H., et al. (2015). Importation and recombination are responsible for the latest emergence of highly pathogenic porcine reproductive and respiratory syndrome virus in China. J. Virol. 89, 10712–10716. doi: 10.1128/JVI.01446-15

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhao, Z., Tang, X., Zhao, X., Zhang, M., Zhang, W., Hou, S., et al. (2014). Tylvalosin exhibits anti-inflammatory property and attenuates acute lung injury in different models possibly through suppression of NF-kappaB activation. Biochem. Pharmacol. 90, 73–87. doi: 10.1016/j.bcp.2014.04.015

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhou, L., Wang, Z., Ding, Y., Ge, X., Guo, X., and Yang, H. (2015). NADC30-like strain of porcine reproductive and respiratory syndrome virus, China. Emerg. Infect. Dis. 21, 2256–2257.

Google Scholar

Zhou, L., Yang, B., Xu, L., Jin, H., Ge, X., Guo, X., et al. (2017). Efficacy evaluation of three modified-live virus vaccines against a strain of porcine reproductive and respiratory syndrome virus NADC30-like. Vet. Microbiol. 207, 108–116. doi: 10.1016/j.vetmic.2017.05.031

PubMed Abstract | CrossRef Full Text | Google Scholar