Corrigendum: Potent Restriction of Sexual Zika Virus Infection by the Lipid Fraction of Extracellular Vesicles in Semen

A Corrigendum on
Potent Restriction of Sexual Zika Virus Infection by the Lipid Fraction of Extracellular Vesicles in Semen

by Wang, R., Gornalusse, G. G., Kim, Y., Pandey, U., Hladik, F., and Vojtech, L. (2020). Front. Microbiol. 11:574054. doi: 10.3389/fmicb.2020.574054

In the original article, there was a mistake in the legend for Figure 4 as published. Figure 4E depicts the effect of pre-incubating liposomes with either ZIKV or cells, but the legend mistakenly says SEV rather than liposomes. The correct legend appears below.

Figure 4. Lipid content of SEV blocks ZIKV infection. (A) The protein content of SEV was denatured by heating at 95° for 8 min. Nanoparticle tracking analysis (NTA) profile comparing heat-treated and untreated SEV showing that the size profiles do not change. Size profiles of liposomes (see part C) are also plotted. (B) Equal amounts of heat-treated or untreated SEV were incubated with ZIKV virions at a 10⁶ SEV:PFU, then added to epithelial cell lines for 1.5 h before washing and assessing ZIKV binding by ddPCR. As a control, ZIKV virions were also heated at 95° for 8 min. Each line is a separate cell line, points are mean of two replicate wells and error bars are standard deviation. Significance by one-way ANOVA with Tukey's multiple comparisons test. **p > 0.01. (C) ZIKV was pre-incubated with 10⁶ SEV or 10⁶ liposomes for 1 h prior to infecting genital epithelial cells. Seventy-two hours post-infection cells were lysed and ZIKV genomes quantified by ddPCR. Each line is the average of duplicate wells from a separate experiment. Reduction in ZIKV genomes was significant by one-way ANOVA with Dunnett's multiple comparisons test. *p < 0.05, and **p < 0.005. (D) The data from C, and additional conditions with 10⁵ SEV or liposomes, are plotted as percent reduction from ZIKV alone. Each symbol represents the average of two technical wells from a different cell line. The horizontal lines are the mean for each condition. (E) Comparison of pre-incubation of either ZIKV or epithelial cells with liposomes on viral infection. Liposomes were pre-incubated with ZIKV (ratio:10⁶ liposome per PFU) or with cells (same amount of liposomes) for 1 h at 37°, then virus was added to cells for 1.5 h. Cells were washed and cell-associated ZIKV genomes quantified by ddPCR. Each symbol represents the average of two technical replicates from different epithelial cell lines. Significance by one-way ANOVA with Dunnett's multiple comparisons test. *p < 0.05.

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.