Changqing He1,2Jiaxing Lei1,2Yaling Yao3Xiangyong Qu1,2Jifa Chen2,4Kailai Xie1Xingju Wang1Qi Yi1Bing Xiao5
Songchang Guo1,2*
Xiaoyan Zou6*- 1Hunan Engineering Research Center of Poultry Production Safety, Hunan Agricultural University, Changsha, China
- 2College of Animal Science and Technology, Hunan Agricultural University, Changsha, China
- 3Huaihua Animal Husbandry and Fishery Affairs Center, Huaihua, China
- 4College of Life Science and Resources and Environment, Yichun University, Yichun, China
- 5Hunan Yunfeifeng Agricultural Co., Ltd., Huaihua, China
- 6College of Veterinary Medicine, Hunan Agricultural University, Changsha, China
The addition of Hermetia illucens larvae meal (HILM) to the feed could contribute to particular antimicrobial and intestinal health in animal husbandry. This study was conducted to investigate the effects of HILM on intestinal morphology and microbial diversity in different intestinal segments of Xuefeng black-bone chickens. All of 432 birds (45 weeks old) were randomly assigned to four equal groups with six replicates and 18 hens in each replicate: (A) basal diet, (B) basal diet with 1% HILM, (C) basal diet with 3% HILM, and (D) basal diet with 5% HILM. The results showed that, compared with the basal diet group, the HILM supplement significantly increased the abundance-based coverage estimator (ACE) and Chao index in cecum (p < 0.05). Diet with 1% HILM significantly increased the villus height (VH) of the duodenum (p < 0.05) and cecum microbial diversity as represented by the Simpson index (p < 0.05). In particular, 1% HILM displayed a markedly increase in the genus unclassified Bacteroidales (cecum, p < 0.05). A basal diet with 3% HILM markedly increased the beneficial genus Romboutsia (jejunum, p < 0.05). Also, principal component analysis (PCA) cluster analysis showed that 3% of HILM was more individual than other groups (p < 0.05). However, 5% HILM decreased the VH and the ratio of villus height to crypt depth (VH/CD) of the jejunum and increased beneficial bacteria such as Staphylococcus (p < 0.05), which was regarded as pathogenetic genera. In conclusion, we found that HILM improved intestinal morphology and increased microbiological diversity and species abundance. Together, dietary supplementation of 1 or 3% HILM might benefit the intestinal morphology and intestinal microbiota of Xuefeng black-bone chicken. However, the addition of 5% HILM could decrease VH and the ratio of VH/CD of the jejunum and increased pathogenetic genera. HILM was an excellent protein substitute for Xuefeng black-bone chickens, which could meet the nutritional requirements under the condition of less feed. These results provide information for HILM meal as an alternative source of soybean meal in Xuefeng black-bone chickens’ feed.
Introduction
In recent years, the demand for high-quality protein in the world has grown tremendously. Being one of the sources with high-quality protein, it has been reported that human demand for poultry meat would increase more rapidly than any other meat product between 2030 and 2050 (the 25th World’s Poultry Congress, 2016). With this upcoming expansion, the resources needed to grow food and feed were becoming increasingly strained because the production of animal and plant proteins required the use of large amounts of land, water, and energy (Kim et al., 2019; Rathnayaka et al., 2021). Therefore, existing feed resources might not be able to support the current growth in poultry production; alternative feeds and feed protein supplements were absolutely needed to replace the current supply and meet the growing demand (De Smet et al., 2018).
Insect, compared with conventional proteins, seemed to be one of the most promising alternatives (Henry et al., 2015; DiGiacomo et al., 2019; Gravel and Doyen, 2020). Hermetia illucens larvae meal (HILM) was characterized by being rich in protein (dry matter >37%) and fat (up to 49%), as well as several macronutrients and micronutrients, which were important for animal development (Smetana et al., 2019). The amino acids of HILM are balanced; most of the amino acid content is similar to fish meals and soybean meals (Barroso et al., 2014). Different from other insects, it was indicated in the prevailing view that HILM products could have particular antimicrobial attributes (Elhag et al., 2017; Fawole et al., 2020; Wang et al., 2020), which might profit from the antimicrobial peptides and chitin in HILM. Furthermore, HILM had a lower environmental impact than other protein sources, making it a potential alternative to regular feed resources for animal production (Wang and Shelomi, 2017). Recently, HILM had been used in the production of livestock (poultry, rabbits, and pigs) and aquaculture species (Gasco et al., 2019).
Xuefeng black-bone chickens, originating from Xuefengshan Mountain and the adjacent region, were the most famous local breed in the Hunan province of China and had been listed as one of the national livestock and poultry genetic resources in China. Being classified into the type of meat-and-egg chicken, Xuefeng black-bone chicken was commonly believed to have medicinal properties and has been used as remedies to enhance the human immune system by providing protein, vitamins, and amino acids (Xie et al., 2020). At present, induced by the switch from quantity to quality of consumers, the market demand for Xuefeng black-bone chicken is increasing (Guo et al., 2021). Moreover, the shortage of protein resources for livestock and poultry feeding drove up the rise of the cost of animal husbandry, which required seeking potential substitutes. Studies have revealed that HILM could be a suitable protein substitution for poultry (Marono et al., 2017; Cutrignelli et al., 2018; Moniello et al., 2019). Nonetheless, its application on Xuefeng black-bone chicken remained poorly understood. In this context, we conducted to explore the effects of HILM on intestinal morphology and microbial diversity in different intestinal segments of the Xuefeng black-bone chicken and provide more detailed information about the capability of HILM as an ideal protein replacement in feed and inform the future diagnosis and therapy of insect meal as a sustainable protein source.
Materials and Methods
All the animals were humanely conducted in accordance with the principles stated by the Chinese guidelines for animal welfare. The experimental procedures received approval from the animal welfare standards of the College of Animal Science and Technology, Hunan Agricultural University.
Animals, Diets, and Experimental Design
A total of 432 female Xuefeng black-bone chickens were randomly divided into four groups (6 replicas of 18 hens) and fed for 56 days. The diets based on corn and soybean meal of the chickens were supplemented with 0, 1, 3, or 5% HILM to partly replace soybean meal and designated as A, B, C, and D groups, respectively. The feeding experiment was the same as our previous study (Liu et al., 2021). The experimental diets were isonitrogenous and isoenergetic, but the proportion of HILM was different.
Sample Collection
At the end of the experiment, three chickens from each group were randomly chosen and killed by cervical dislocation. After killing, the digesta samples from the jejunum and cecum were collected in sterilized plastic tubes and then immediately stored in liquid nitrogen to investigate intestinal microflora. Additionally, segments (10 cm in length) of the middle of the duodenum and jejunum were excised and washed with phosphate-buffered saline until no digesta were visible. The segments were fixed with 4% paraformaldehyde for more than 24 h to make intestinal sections.
Experimental Parameters Analysis
Intestinal Morphology
Five-μm sections were cut from the paraffin-embedded intestinal segments after removing the fixation solution and then stained with hematoxylin and eosin for light microscopy. Zeiss microscope (Axio Imager, A1) and the MShot Image Analysis System were used to take photographs for sections. The villus height (VH) and the crypt depth (CD) were used in five typical fields to measure (Wang and Hogan, 2019).
Intestinal Microflora
Genomic DNA was extracted from chyme samples using a kit (DP328, Tiangen Biochemical Technology Co., Ltd., China) (Xia et al., 2020). The samples of qualified DNA were sent to Shanghai Majorbio Bio-pharm Technology Co., Ltd. (China) for polymerase chain reaction amplification and sequencing analysis.
Using quantitative DNA as a template, the 16S rRNA gene V3–V4 region was amplified by primer pairs 338F (5′-ACTCCTACGGGAGCAGAC-3′) and 806R (5′-GGACTACHVGGGTWTCTA-3′). The purified, amplified products were sequenced on the Novaseq PE250 platform (Illumina, San Diego, United States) using equimolar and paired-end sequencing according to MajorBio Biopharma Technologies, Inc. (Shanghai, China) standard protocol.
The original sequence was demultiplexed and quality filtered using FASTP version 0.20.0 (Chen et al., 2018) and merged using Flash version 1.2.7 (Magoc and Salzberg, 2011). The criteria are as follows: (a) the 300-bp reads were truncated at any site receiving an average quality score of <20 over a 50-bp sliding window, and the truncated reads shorter than 50 bp were discarded; reads containing ambiguous characters were also discarded; (b) Only overlap sequences greater than 10 bp are spliced according to overlapping sequences. The maximum mismatch in the overlapping area is 0.2. Reads that cannot be assembled are discarded; (c) samples were distinguished according to the barcode and primers, and the sequence direction was adjusted. The barcode matching was accurate, and the two nucleotides in the primer matching did not match. UPARSE version 7.1 was used to cluster operational taxonomic units (OTU) with a similarity cutoff rate of 97% (Edgar, 2013). RDP Classifier version 2.2 was used to classify each OTU representative sequence, and Silva V132 was used for classification and identification.
Statistical Analysis
One-way analysis of variance in SPSS 22.0 software (IBM SPSS, United States) was used for the statistical analyses. The analysis results were expressed as the arithmetic mean and standard error. Differences were considered to be significant at p < 0.05 and highly significant at p < 0.01. The Majorbio Cloud Platform1 was used to analyze intestinal microflora.
Results
Intestinal Morphology
To determine whether HILM diets were associated with the intestinal morphology of Xuefeng black-bone chickens, we measured the VH and CD of the duodenum and jejunum (Figure 1). In the duodenum, the VH of group B was significantly higher than groups A and D (p < 0.05). However, no significant difference was observed in the CD and the ratio of VH to CD (VH/CD) among four treatments (p > 0.05). In the jejunum, the VH of group B was the highest, which was markedly higher than groups C and D (p < 0.05). The CD decreased significantly in group D, compared with group A (p < 0.05).
Figure 1. Effects of HILM on intestinal morphology of Xuefeng black-bone chicken. (A) Representative pictures of hematoxylin and eosin staining (40×, scale bar: 20 μm); VH of duodenum (B) and jejunum (E); CD of duodenum (C) and jejunum (F); VH/CD of duodenum (D) and jejunum (G). Results shown are means ± SEM (n = 12). ∗p < 0.05, ∗∗p < 0.01. VH, villus height; CD, crypt depth; VH/CD, ratio of villus height to crypt depth; HILM, Hermetia illucens larvae meal.
Production Performance
The production performance was shown in Table 2 of our previous study (Liu et al., 2021). In the overall period (from day 1 to 56 of the experiment), 3% HILM increased the egg weight (p < 0.05), feed intake (p < 0.05), and egg production (p < 0.05), meanwhile decreased the feed conversion ratio (p < 0.05) in a linear or quadratic manner.
Operational Taxonomic Unit Analysis and Venn Diagram of the Difference in Operational Taxonomic Unit Distributions Between Groups
In our study, a total of four groups (each group with two intestinal segments) were investigated, and each sample contained large numbers of enriched OTUs (Figure 2). According to OTU classification, we calculated the same number of OTUs between groups and then pointed them in the Venn graph. Each color block in the Venn graph represents a group, intersecting sections represent OTUs shared with adjacent groups, and one number means one group. The OTUs of the jejunum were as follows (Figure 2A): JA, 53; JB, 64; JC, 92; and JD, 85. Uniform OTUs in the jejunum among groups were as follows: JA and JB, 32; JA and JC, 59; JA and JD, 13; JB and JC, 44; JB and JD, 30; JC and JD, 54; JA, JB, and JC, 83; JA, JB, and JD, 36; JA, JC, and JD, 32; JB, JC, and JD, 53; and JA, JB, JC, and JD, 627. The OTUs in the duodenum were as follows (Figure 2B): CA, 23; CB, 16; CC, 24; and CD, 46. Uniform OTUs in the duodenum among groups were as follows: CA and CB, 8; CA and CC, 10; CA and CD, 28; CB and CC, 20; CB and CD, 22; CC and CD, 44; CA, CB, and CC, 24; CA, CB, and CD, 20; CA, CC, and CD, 42; CB, CC, and CD, 77; and CA, CB, CC, and CD, 733.
Figure 2. Shared and specific operational taxonomic unit (OTUs) in different intestinal sections of different groups (A: jejunum; B: cecum). JA, jejunum of 0% HILM; JB, jejunum of 1% HILM; JC, jejunum of 3% HILM; JD, jejunum of 5% HILM; CA, cecum of 0% HILM; CB, cecum of 1% HILM; CC, cecum of 3% HILM; CD, cecum of 5% HILM.
Alpha Diversity
The abundance-based coverage estimator (ACE) index, Chao index, Shannon index, and Simpson index were selected to analyze diversity (Figure 3). Although dietary HILM did not influence bacterial diversity in the jejunum, the richness of the intestinal microbiota increased in group C, but the community richness and diversity decreased in group D (Figure 3A, p > 0.05). However, in the cecum, the ACE index and Chao index of group D were significantly higher than those of group A (p < 0.05) and group B (p < 0.01). The Simpson index was the lowest, which was significantly higher than that of group A (p < 0.05). There were no significant differences observed among the groups in the Shannon index (p > 0.05).
Figure 3. Alpha diversity of four groups in jejunum (A) and cecum (B). Significant difference between samples and marks with significant differences between two groups. JA, jejunum of 0% HILM; JB, jejunum of 1% HILM; JC, jejunum of 3% HILM, JD: jejunum of 5% HILM; CA, cecum of 0% HILM; CB, cecum of 1% HILM; CC, cecum of 3% HILM; CD, cecum of 5% HILM.
Heatmap Analysis–Phylum Level
To analyze the effects of HILM on the microbial community, a heatmap was used to assess the composition of microorganisms by color shades among the four groups (each group with two intestinal segments). The results showed that Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, Spirochaetota, Desulfobacterota, Campilobacterota, WPS-2, and Synergistetes accounted for 98.58% of the microbial population (Figure 4A). Among those, Bacteroidetes and Firmicutes were the main phyla in the jejunum, accounting for 83.37%. As shown in Figure 4C, the Deferribacterota and Fusobacteriota phyla of group A were markedly improved (p < 0.05).
Figure 4. Heatmap chart of top 20 phyla in different intestinal segments (A: jejunum; B: cecum) and comparison of differences between groups of top 20 phyla (C: jejunum; D: cecum). One-way analysis of variance, JA, jejunum of 0% HILM; JB, jejunum of 1% HILM; JC, jejunum of 3% HILM; JD, jejunum of 5% HILM; CA, cecum of 0% HILM; CB, cecum of 1% HILM; CC, cecum of 3% HILM; CD, cecum of 5% HILM.
As shown in Figure 4B, the dominant bacterial phyla of the cecum were Firmicutes, Bacteroidetes, Desulfobacterota, Synergistetes, Spirochaetota, and WPS-2, accounting for 99.12% of the microbial population. Among those, Bacteroidetes and Firmicutes were the main phyla in the jejunum, accounting for 91.78%. The Campilobacterota phylum of group C was significantly elevated (p < 0.05); however, there were no significant differences among other microorganisms (p > 0.05, Figure 4D).
Heatmap Analysis–Genus Level
As shown in Figure 5A, the dominant genus of the jejunum was Bacteroides, and the second genus was Lactobacillus. At the genus level, the analysis results showed that compared with groups A, B, and C, the abundances of Staphylococcus in group D significantly increased. The abundances of Romboutsia increased in group C (p < 0.05, Figure 5C). However, in group A, we found that the abundances of Megamonas were significantly higher than others (p < 0.05).
Figure 5. Heatmap chart of the top 20 genera in different intestinal segments (A: jejunum; B: cecum) and comparison of differences between groups of the top 20 phyla (C: jejunum; D: cecum). One-way analysis of variance, JA, jejunum of 0% HILM; JB, jejunum of 1% HILM; JC, jejunum of 3% HILM; JD, jejunum of 5% HILM; CA, cecum of 0% HILM; CB, cecum of 1% HILM; CC, cecum of 3% HILM; CD, cecum of 5% HILM.
As shown in Figure 5B, the dominant genus of the cecum was Bacteroides, and the next genus was Rikenellaceae. In group B, we found that the abundances of the unclassified Bacteroidales were significantly higher than the other groups (p < 0.05, Figure 5D). However, there was no significant change in the intestinal microflora in the cecum (p > 0.05).
Principal Component Analysis Cluster Analysis
To analyze the effects of HILM on the microbial community, principal component analysis (PCA) used weighted UniFrac distances, which were calculated based on the OTU species and relative abundance of the samples (Figure 6). The results displayed significant differences between microbial communities in group C and group D of the jejunum but no significant differences between the microbial communities of groups A and B (Figure 6A). It suggested that the feed additives significantly influenced the gut microbiota distribution in chicken. Figure 6B showed that the degree of clustering of individuals in groups A and B was high, whereas the degree of clustering of individuals in groups C and D was low, indicating that the similarity within groups A and B was higher than in other groups. There were no significant differences among the microbial communities in the cecum (p > 0.05).
Figure 6. Cluster analysis by principal coordinate analysis. (A) jejunum; (B) cecum. JA, jejunum of 0% HILM; JB, jejunum of 1% HILM; JC, jejunum of 3% HILM; JD, jejunum of 5% HILM; CA, cecum of 0% HILM; CB, cecum of 1% HILM; CC, cecum of 3% HILM; CD, cecum of 5% HILM. X- and Y-axes represent two selected main coordinate axes, and percentage represents interpretation value of main coordinate axis to difference of sample composition; scales of the X- and Y-axes are relative distances and have no practical significance; points of different colors or shapes represent samples of different groups; the closer the two sample points are, the more similar the species composition of the two samples.
Discussion
HILM contains higher or similar protein content and richer essential amino acid content (Yu et al., 2020). During recent decades, many studies indicated that the significant effects of HILM on intestinal health (Weththasinghe et al., 2021). Intestinal morphology and intestinal microbiota are critical to maintaining ecosystem stability and performance. HILM is a reasonable protein feed additive. The current study evaluated the effect of HILM on villus morphology of the jejunum and duodenum and the comparison of OTUs of microorganisms, alpha diversity, PCA cluster analysis, and microbial species composition analysis of the jejunum and cecum.
The rapid growth of broilers was due to the great potential of the small intestinal cells to absorb nutrients and the efficient conversion of nutrients to muscles (Mishra and Jha, 2019; Liu et al., 2020). Modifications of intestinal morphology, mainly increasing VH and the VH/CD in the duodenum and jejunum, could improve nutrient absorption (Schiavone et al., 2018; Hajimohammadi et al., 2020). Small intestinal VH determined the functional maturity of villus absorptive cells while the VH increases; the intestinal surface area enabled more efficient absorption of available nutrients (Li et al., 2019; Ramlucken et al., 2019). In this study, the supplementation of 1% HILM significantly increased the VH of the duodenum, and dietary 1 and 3% HILM supplementation had no damage effect in the duodenum and jejunum. In conclusion, dietary supplementation of HILM could improve nutrient absorption, maintain the normal intestinal morphology, and had effects on VH, CD, and VH/CD. However, dietary supplementation of 5% HILM reduced the VH of the jejunum and duodenum and VH/CD of the jejunum, consistent with the adverse effects of protein source substitution on small intestinal morphology in broilers (Biasato et al., 2018b). Interestingly, the CD of dietary 5% HILM supplementation in the jejunum decreased, which contrasted with earlier studies (Ibitoye et al., 2019). This might be related to the high chitin content in HILM, which may cause damage to the intestinal structure and reduce nutrient digestibility (Kroeckel et al., 2012). The supplementation of chitinase in diets supplemented with HILM might improve the effect, which needed to be studied in the future.
The intestinal tracts of poultry were complex, diverse, and dynamic microbial communities, which played an important role in host health and production performance. In the process of nutrition, metabolism, physiology, and immunity, the microbial community could promote digestion and absorption of nutrients, stimulate the immune response of the host, and enhance resistance to infection (Shi et al., 2020). However, gut microbiota also had direct and indirect harmful effects on decreased digestibility, increased cell turnover rate, and production of toxic metabolites, which might also lead to the decreased growth performance of chickens (Wang et al., 2017; Yadav and Jha, 2019). It was necessary to sufficiently understand the composition and diversity of the intestinal microbiome to further promote poultry growth and intestinal health. In this study, 16S recombinant DNA gene sequencing was used to detect the microbial diversity and community compositions of bacteria in the jejunum and cecum of Xuefeng black-bone chickens fed with different doses of HILM diets. The OTU number of dietary 3% HILM supplementation was the highest in the jejunum, which implied that the 3% HILM to the diet increased the microbial diversity of the jejunum. However, none of the groups in both the jejunum and cecum had the unique OTU number, indicating that dietary HILM had no effect but maintained species richness.
In different parts of the intestine, due to different intestinal structures, pH values, and diet status, bacterial populations vary greatly. Alpha diversity analysis analyzed species diversity in independent samples (Cui et al., 2021). The high diversity of intestinal bacteria is conducive to maintaining intestinal stability. In this study, we investigated the ACE, Chao, Shannon, and Simpson indexes in the jejunum and cecum of the dietary HILM to Xuefeng black-bone chickens. The results showed that dietary 3 and 5% HILM supplementation significantly increased the ACE and Chao indexes, and 1% HILM supplementation significantly decreased the Simpson index in the cecum, consistent with the report that highly nutritious diets could increase diversity indexes (He et al., 2020). Although there were no significantly differences of alpha diversity index in the jejunum, 3% HILM had the highest ACE, Chao, and Shannon indexes and the lowest Simpson index. Accordingly, the alpha diversity index was used as the community richness index. The higher the ACE, Chao, and Shannon indexes, the higher the community richness, whereas the Simpson index was the opposite. Improving the alpha index of dietary HILM might increase the stability between communities and closer the connections between communities.
Robust and balanced compositions of intestinal microflora were required to support health and growth. Microorganisms could benefit the host by affecting nutrient digestion and disease resistance, but an overgrowth of gut microbial could affect ecosystem balance and destroy gut integrity to initiate intestinal inflammation (Saracila et al., 2018). HILM, as a kind of feed resource based on insects, which could maintain intestinal microorganism health, has been reported in animal feed production (Leni et al., 2020). Based on the results of species annotation, we analyzed the microbial composition of the cecum and jejunum at the phylum and genus levels to evaluate the role of HILM in maintaining homeostasis.
At the phylum level of the jejunum and cecum microbial composition, both Bacteroidetes and Firmicutes were the largest phyla, which accounted for >80% of all the microbial community detected in our study, and Bacteroidetes and Firmicutes accounted for >90% in the cecum. This was consistent with many previous studies suggesting that Firmicutes and Bacteroidetes made up the majority of the microbial communities in chickens (accounting for >80%) at the phylum level, and these bacteria affected energy production and metabolism (Shang et al., 2018; Herrero-Encinas et al., 2020). Bacteroidetes and Firmicutes were the common colonizers of the chicken intestinal, which were considered to be potentially beneficial autochthonous bacteria (Borda-Molina et al., 2020). Cui et al. (2021) found that these two kinds of a dominant phylum of the gut community played an important role in improving intestinal diseases, inhibiting the proliferation of harmful intestinal bacteria, and producing short-chain fatty acids, which reduced luminal pH and regulated the microbial composition (stimulating the growth of beneficial bacteria, such as Bifidobacterium) (Dong et al., 2017; Ratajczak et al., 2019). At the phylum level of the jejunum, we found that HILM supplementation decreased the number of Deferribacterota and Fusobacteriota phyla. Deferribacterota has been found in healthy mice and humans. However, the relatively small proportion of intestinal microorganisms and their role and function have been rarely reported (Lewis et al., 2021). Also, Fusobacteria, anaerobic Gram-negative rods, were rare agents of severe human diseases that had repeatedly noted their link to colorectal cancer. It was indicated that HILM supplementation to the diet could alleviate damage and improve the intestinal microbiota disorders caused by the disease. At the phylum level of the cecum, the Campilobacterota phyla were greatly improved with 3% HILM supplementation, which was not a usual report with the phylum by poultry studies under natural or captivity conditions. Although some members of this phylum might cause diseases in wild and domestic animals, they were considered to be nonpathogenic and were frequently isolated from healthy birds (Zhao Y. et al., 2019; Chen et al., 2020; Maraci et al., 2021). It was showed that HILM could affect intestinal microbiota, either directly or indirectly, by modulating jejunal and cecal microbial compositions at the phylum level, especially a diet with 3% HILM supplementation.
At the genus level, the dominant bacteria in the gut were remodeled. Different from phyla, the dominant taxa in chicken cecal microbiota was controversial (Biasato et al., 2018a). Costa et al. (2017) found that the most predominant genera in the cecum of broilers were Clostridium, Ruminococcus, Lactobacillus, and Bacteroides. Callaway et al. (2009) found that Prevotella was the most abundant genus. In particular, the Bacteroides genus has been reported as the most predominant member of the cecal microbiota of Bermuda free-range broilers. In this study, the genus Bacteroides was the positive bacterium in the jejunum and cecum, which is in line with the previous finding that a higher number of Bacteroides enables larger efficiency of gut microbiota in extracting energy from the diet (Yue et al., 2019). Of interest, in our findings, the genus Lactobacillus was the second leading bacterium in the jejunum. Li et al. (2020) also found that Lactobacillus consisted in the gut of healthy pigs. Lactobacillus had a strong antibacterial activity, which enhanced the health of the chickens by inhibiting the growth of pathogens through competitive exclusion in the gastrointestinal tract (Ahmed et al., 2019). In addition, dietary 3% HILM increased the genus Romboutsia, a valuable intestinal biomarker in maintaining the health of the host (Mangifesta et al., 2018; Zhang et al., 2021). However, the 5% HILM supplementation increased the genus Staphylococcus and decreased the genus Megamonas in the jejunum. Megamonas was a genus of Firmicutes bacteria, which has been reported that it acted as a hydrogen sink in the gut of broilers by increasing the production of short-chain fatty acids (Chen et al., 2019). In general, most Staphylococcus were nonpathogenic bacteria, but a few might cause disease. The result showed that high-level protein might result in the decrease of beneficial microbes, leading to an imbalance of the jejunum microbiota. Diet was one of the most important factors that affected the gut microbiota (Makki et al., 2018). The components of dietary protein affected the composition of gut microbiota, and higher levels could lead to an increase in pathogenic microorganisms, thereby increasing the associated risk of metabolic diseases (Zhao J. et al., 2019; Sedgh-Gooya et al., 2021). Moreover, in the cecum, the genus Lactobacillus was the second leading bacterium. Dalle Zotte et al. (2021) fattened quails with full-fat or defatted silkworm pupa meal to observe the cecal microbiome and found that the genus Rikenellaceae was increased, which was consistent with our results. Rikenellaceae has been reported to promote starch decomposition in raw potato starch-fed mice (Bang et al., 2019). Meanwhile, we observed that the genus unclassified Bacteroidales in 1% HILM of the cecum was significantly increased. The results showed that dietary 1 or 3% HILM could increase the number of beneficial microflora, maintaining the jejunal and cecal microflora stability. However, 5% HILM supplementation might have no beneficial effect on the intestine.
The main intention of PCA cluster analysis was to investigate the similarity of community structure among varied samples, to observe the differences among samples by sorting the samples by decomposing the community data structure (Fang et al., 2016). In this study, the weighted UniFrac plot showed that the jejunal microbial community of groups C and D was highly isolated from groups A and B. Cui et al. (2021) reported that the grouping effect was obvious and could effectively stabilize the progenitor of intestinal flora. It indicated that a diet supplemented with 3 and 5% HILM could change the species and abundance of main intestinal microflora and stabilize the composition of intestinal microflora. In addition, diet HILM had little effect on the difference of community members and the abundance of community members of the cecum.
Our previous data demonstrated that dietary supplementation of 3% HILM in broiler breeders could significantly increase feed intake and egg weight. Additionally, HILM could strengthen the activity of total-superoxide dismutase and the level of antibody to avian influenza virus of plasma. The data of this study displayed that dietary supplementation of 1 or 3% HILM benefited the intestinal morphology and intestinal microbiota of Xuefeng black-bone chicken. Together, 3% HILM dietary supplementation might improve intestinal morphology and regulate microbiota, increasing feed intake, egg weight, and disease resistance. Hence, HILM would be a suitable substitute for plant protein in the diet of Xuefeng black-bone chicken.
Conclusion
Dietary supplementation of HILM to Xuefeng black-bone chicken diets improved intestinal morphology, alpha diversity, and beneficial flora content (Campilobacterota, Romboutsia, unclassified Bacteroidales). Among the three additive quantities, the addition of 1% HILM increased the VH of the duodenum and decreased the Simpson index of the cecum, and the addition of 3% HILM stabilized the composition of the jejunum. However, the addition of 5% HILM decreased the VH and the ratio of VH/CD of the jejunum and increased pathogenetic genera (Staphylococcus). Dietary 1 or 3% HILM might benefit intestinal morphology and intestinal microbiota of Xuefeng black-bone chicken. HILM could be an alternative protein supplement to meet the growing demand in poultry production.
Data Availability Statement
The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found below: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA747901/.
Ethics Statement
The animal study was reviewed and approved by the Animal Care and Use Committee of Hunan Agricultural University (HUNAU2020005).
Author Contributions
CH, JC, and XZ designed the experiment. JL and YY performed the experiment. KX and XW collected the samples. QY, BX, XQ, and SG analyzed the samples and data. SG, CH, and XZ conceptualized the manuscript, compiled all of the information, and prepared the manuscript. All authors read and approved the final manuscript.
Funding
This study was funded by the start-up funds from Hunan Agricultural University, the National Innovation Training Program for College Students (G)SCX1802, the Hunan Science and Technology Correspondent Innovation and Entrepreneurship Project (2020NK4155), and the National Natural Science Foundation of China (32072711).
Conflict of Interest
BX was employed by Hunan Yunfeifeng Agricultural Co. Ltd.
The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
The reviewer RF declared a shared affiliation, with no collaboration, with the authors, to the handling editor at the time of the review.
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Footnotes
References
Ahmed, Z., Vohra, M. S., Khan, M. N., Ahmed, A., and Khan, T. A. (2019). Antimicrobial role of Lactobacillus species as potential probiotics against enteropathogenic bacteria in chickens. J. Infect. Dev. Ctries. 13, 130–136. doi: 10.3855/jidc.10542
Bang, S. J., Lee, E. S., Song, E. J., Nam, Y. D., Seo, M. J., Kim, H. J., et al. (2019). Effect of raw potato starch on the gut microbiome and metabolome in mice. Int. J. Biol. Macromol. 133, 37–43. doi: 10.1016/j.ijbiomac.2019.04.085
Barroso, F. G., de Haro, C., Sanchez-Muros, M. J., Venegas, E., Martinez-Sanchez, A., and Perez-Banon, C. (2014). The potential of various insect species for use as food for fish. Aquaculture 422, 193–201. doi: 10.1016/j.aquaculture.2013.12.024
Biasato, I., Ferrocino, I., Biasibetti, E., Grego, E., Dabbou, S., Sereno, A., et al. (2018a). Modulation of intestinal microbiota, morphology and mucin composition by dietary insect meal inclusion in free-range chickens. BMC Vet. Res. 14:383. doi: 10.1186/s12917-018-1690-y
Biasato, I., Gasco, L., De Marco, M., Renna, M., Rotolo, L., Dabbou, S., et al. (2018b). Yellow mealworm larvae (Tenebrio molitor) inclusion in diets for male broiler chickens: effects on growth performance, gut morphology, and histological findings. Poult. Sci. 97, 540–548. doi: 10.3382/ps/pex308
Borda-Molina, D., Matis, G., Mackei, M., Neogrady, Z., Huber, K., Seifert, J., et al. (2020). Caeca microbial variation in broiler chickens as a result of dietary combinations using two cereal types, supplementation of crude protein and sodium butyrate. Front. Microbiol. 11:617800. doi: 10.3389/fmicb.2020.617800
Callaway, T. R., Dowd, S. E., Wolcott, R. D., Sun, Y., McReynolds, J. L., Edrington, T. S., et al. (2009). Evaluation of the bacterial diversity in cecal contents of laying hens fed various molting diets by using bacterial tag-encoded FLX amplicon pyrosequencing. Poult. Sci. 88, 298–302. doi: 10.3382/ps.2008-00222
Chen, C. Y., Chen, C. K., Chen, Y. Y., Fang, A., Shaw, G. T., Hung, C. M., et al. (2020). Maternal gut microbes shape the early-life assembly of gut microbiota in passerine chicks via nests. Microbiome 8:129. doi: 10.1186/s40168-020-00896-9
Chen, S., Zhou, Y., Chen, Y., and Gu, J. (2018). fastp: an ultra-fast all-in-one FASTQ preprocessor. Bioinformatics 34, 884–890. doi: 10.1093/bioinformatics/bty560
Chen, Y., Ni, J., and Li, H. (2019). Effect of green tea and mulberry leaf powders on the gut microbiota of chicken. BMC Vet Res 15:77. doi: 10.1186/s12917-019-1822-z
Costa, M. C., Bessegatto, J. A., Alfieri, A. A., Weese, J. S., Filho, J. A., and Oba, A. (2017). Different antibiotic growth promoters induce specific changes in the cecal microbiota membership of broiler chicken. PLoS One 12:e0171642. doi: 10.1371/journal.pone.0171642
Cui, Y., Han, C., Li, S., Geng, Y., Wei, Y., Shi, W., et al. (2021). High-throughput sequencing-based analysis of the intestinal microbiota of broiler chickens fed with compound small peptides of Chinese medicine. Poult. Sci. 100:100897. doi: 10.1016/j.psj.2020.11.066
Cutrignelli, M. I., Messina, M., Tulli, F., Randazzo, B., Olivotto, I., Gasco, L., et al. (2018). Evaluation of an insect meal of the Black Soldier Fly (Hermetia illucens) as soybean substitute: intestinal morphometry, enzymatic and microbial activity in laying hens. Res. Vet. Sci. 117, 209–215. doi: 10.1016/j.rvsc.2017.12.020
Dalle Zotte, A., Singh, Y., Squartini, A., Stevanato, P., Cappellozza, S., Kovitvadhi, A., et al. (2021). Effect of a dietary inclusion of full-fat or defatted silkworm pupa meal on the nutrient digestibility and faecal microbiome of fattening quails. Animal 15:100112. doi: 10.1016/j.animal.2020.100112
De Smet, J., Wynants, E., Cos, P., and Van Campenhout, L. (2018). Microbial community dynamics during rearing of black soldier fly larvae (Hermetia illucens) and impact on exploitation potential. Appl. Environ. Microbiol. 84:e02722–17. doi: 10.1128/AEM.02722-17
DiGiacomo, K., Akit, H., and Leury, B. J. (2019). Insects: a novel animal-feed protein source for the Australian market. Anim. Prod. Sci. 59, 2037–2045. doi: 10.1071/an19301
Dong, X. Y., Azzam, M. M. M., and Zou, X. T. (2017). Effects of dietary threonine supplementation on intestinal barrier function and gut microbiota of laying hens. Poult. Sci. 96, 3654–3663. doi: 10.3382/ps/pex185
Edgar, R. C. (2013). UPARSE: highly accurate OTU sequences from microbial amplicon reads. Nat. Methods 10, 996–998. doi: 10.1038/nmeth.2604
Elhag, O., Zhou, D., Song, Q., Soomro, A. A., Cai, M., Zheng, L., et al. (2017). Screening, expression, purification and functional characterization of novel antimicrobial peptide genes from Hermetia illucens (L.). PLoS One 12:e0169582. doi: 10.1371/journal.pone.0169582
Fang, X., Wang, X., Yang, S., Meng, F., Wang, X., Wei, H., et al. (2016). Evaluation of the microbial diversity in amyotrophic lateral sclerosis using high-throughput sequencing. Front. Microbiol. 7:1479. doi: 10.3389/fmicb.2016.01479
Fawole, F. J., Adeoye, A. A., Tiamiyu, L. O., Ajala, K. I., Obadara, S. O., and Ganiyu, I. O. (2020). Substituting fishmeal with Hermetia illucens in the diets of African catfish (Clarias gariepinus): effects on growth, nutrient utilization, haemato-physiological response, and oxidative stress biomarker. Aquaculture 518:734849. doi: 10.1016/j.aquaculture.2019.734849
Gasco, L., Biasato, I., Dabbou, S., Schiavone, A., and Gai, F. (2019). Animals fed insect-based diets: state-of-the-art on digestibility, performance and product quality. Animals (Basel) 9:170. doi: 10.3390/ani9040170
Gravel, A., and Doyen, A. (2020). The use of edible insect proteins in food: challenges and issues related to their functional properties. Innov. Food Sci. Emerg. Technol. 59:102272. doi: 10.1016/j.ifset.2019.102272
Guo, S. C., Lei, J. X., Liu, L. L., Qu, X. Y., Li, P., Liu, X., et al. (2021). Effects of Macleaya cordata extract on laying performance, egg quality, and serum indices in Xuefeng black-bone chicken. Poult. Sci. 100:9. doi: 10.1016/j.psj.2021.101031
Hajimohammadi, A., Mottaghitalab, M., and Hashemi, M. (2020). Effects of microbial fermented sesame meal and enzyme supplementation on the intestinal morphology, microbiota, pH, tibia bone and blood parameters of broiler chicks. Ital. J. Anim. Sci. 19, 457–467. doi: 10.1080/1828051x.2020.1755378
He, Y., Yang, Y., Dong, Y., Ito, K., and Zhang, B. (2020). Highly nutritious diet resists Salmonella typhimurium infections by improving intestinal microbiota and morphology in broiler chickens. Poult. Sci. 99, 7055–7065. doi: 10.1016/j.psj.2020.09.073
Henry, M., Gasco, L., Piccolo, G., and Fountoulaki, E. (2015). Review on the use of insects in the diet of farmed fish: past and future. Anim. Feed Sci. Technol. 203, 1–22. doi: 10.1016/j.anifeedsci.2015.03.001
Herrero-Encinas, J., Blanch, M., Pastor, J. J., Mereu, A., Ipharraguerre, I. R., and Menoyo, D. (2020). Effects of a bioactive olive pomace extract from Olea europaea on growth performance, gut function, and intestinal microbiota in broiler chickens. Poult. Sci. 99, 2–10. doi: 10.3382/ps/pez467
Ibitoye, E. B., Lokman, I. H., Hezmee, M. N. M., Goh, Y. M., Zuki, A. B. Z., Jimoh, A. A., et al. (2019). Gut health and serum growth hormone levels of broiler chickens fed dietary chitin and chitosan from cricket and shrimp. Poult. Sci. 98, 745–752. doi: 10.3382/ps/pey419
Kim, S. W., Less, J. F., Wang, L., Yan, T., Kiron, V., Kaushik, S. J., et al. (2019). Meeting global feed protein demand: challenge, opportunity, and strategy. Annu. Rev. Anim. Biosci. 7, 221–243. doi: 10.1146/annurev-animal-030117-014838
Kroeckel, S., Harjes, A. G. E., Roth, I., Katz, H., Wuertz, S., Susenbeth, A., et al. (2012). When a turbot catches a fly: evaluation of a pre-pupae meal of the black soldier fly (Hermetia illucens) as fish meal substitute–growth performance and chitin degradation in juvenile turbot (Psetta maxima). Aquaculture 364, 345–352. doi: 10.1016/j.aquaculture.2012.08.041
Leni, G., Prandi, B., Varani, M., Faccini, A., Caligiani, A., and Sforza, S. (2020). Peptide fingerprinting of Hermetia illucens and Alphitobius diaperinus: identification of insect species-specific marker peptides for authentication in food and feed. Food Chem. 320:126681. doi: 10.1016/j.foodchem.2020.126681
Lewis, W. H., Tahon, G., Geesink, P., Sousa, D. Z., and Ettema, T. J. G. (2021). Innovations to culturing the uncultured microbial majority. Nat. Rev. Microbiol. 19, 225–240. doi: 10.1038/s41579-020-00458-8
Li, X. J., Wang, M., Xue, Y., Duan, D., Li, C., Han, X., et al. (2020). Identification of microflora related to growth performance in pigs based on 16S rRNA sequence analyses. AMB Express 10:192. doi: 10.1186/s13568-020-01130-3
Li, Y., Lu, X., Wu, H., Xia, M., Hou, Q., Hu, W., et al. (2019). The effect of dietary supplementation of low crude protein on intestinal morphology in pigs. Res. Vet. Sci. 122, 15–21. doi: 10.1016/j.rvsc.2018.11.013
Liu, J. B., Yan, H. L., Zhang, Y., Hu, Y. D., and Zhang, H. F. (2020). Effects of stale maize on growth performance, immunity, intestinal morphology and antioxidant capacity in broilers. Asian-Australas. J. Anim. Sci. 33, 605–614. doi: 10.5713/ajas.19.0224
Liu, X., Liu, X., Yao, Y., Qu, X., Chen, J., Xie, K., et al. (2021). Effects of different levels of Hermetia illucens larvae meal on performance, egg quality, yolk fatty acid composition and oxidative status of laying hens. Ital. J. Anim. Sci. 20, 256–266. doi: 10.1080/1828051x.2021.1878946
Magoc, T., and Salzberg, S. L. (2011). FLASH: fast length adjustment of short reads to improve genome assemblies. Bioinformatics 27, 2957–2963. doi: 10.1093/bioinformatics/btr507
Makki, K., Deehan, E. C., Walter, J., and Backhed, F. (2018). The impact of dietary fiber on gut microbiota in host health and disease. Cell Host Microbe 23, 705–715. doi: 10.1016/j.chom.2018.05.012
Mangifesta, M., Mancabelli, L., Milani, C., Gaiani, F., de’Angelis, N., de’Angelis, G. L., et al. (2018). Mucosal microbiota of intestinal polyps reveals putative biomarkers of colorectal cancer. Sci. Rep. 8:13974. doi: 10.1038/s41598-018-32413-2
Maraci, O., Antonatou-Papaioannou, A., Junemann, S., Castillo-Gutierrez, O., Busche, T., Kalinowski, J., et al. (2021). The gut microbial composition is species-specific and individual-specific in two species of estrildid finches, the bengalese finch and the zebra finch. Front. Microbiol. 12:619141. doi: 10.3389/fmicb.2021.619141
Marono, S., Loponte, R., Lombardi, P., Vassalotti, G., Pero, M. E., Russo, F., et al. (2017). Productive performance and blood profiles of laying hens fed Hermetia illucens larvae meal as total replacement of soybean meal from 24 to 45 weeks of age. Poult. Sci. 96, 1783–1790. doi: 10.3382/ps/pew461
Mishra, B., and Jha, R. (2019). Oxidative stress in the poultry gut: potential challenges and interventions. Front. Vet. Sci. 6:60. doi: 10.3389/fvets.2019.00060
Moniello, G., Ariano, A., Panettieri, V., Tulli, F., Olivotto, I., Messina, M., et al. (2019). Intestinal morphometry, enzymatic and microbial activity in laying hens fed different levels of a Hermetia illucens larvae meal and toxic elements content of the insect meal and diets. Animals (Basel) 9:86. doi: 10.3390/ani9030086
Ramlucken, U., Ramchuran, S. O., Moonsamy, G., Lalloo, R., Thantsha, M. S., and Jansen van Rensburg, C. (2019). A novel Bacillus based multi-strain probiotic improves growth performance and intestinal properties of Clostridium perfringens challenged broilers. Poult. Sci. 99, 331–341. doi: 10.3382/ps/pez496
Ratajczak, W., Ryl, A., Mizerski, A., Walczakiewicz, K., Sipak, O., and Laszczynska, M. (2019). Immunomodulatory potential of gut microbiome-derived short-chain fatty acids (SCFAs). Acta Biochim. Pol. 66, 1–12. doi: 10.18388/abp.2018_2648
Rathnayaka, S. D., Selvanathan, S., and Selvanathan, E. A. (2021). Demand for animal-derived food in selected Asian countries: a system-wide analysis. Agric. Econ. 52, 97–122. doi: 10.1111/agec.12609
Saracila, M., Criste, R. D., Panaite, T. D., Vlaicu, P. A., Tabuc, C., Turcu, R. P., et al. (2018). Artemisia annua as phytogenic feed additive in the diet of broilers (14-35 Days) reared under heat stress (32 °C). Braz. J. Poult. Sci. 20, 825–832. doi: 10.1590/1806-9061-2018-0772
Schiavone, A., Dabbou, S., De Marco, M., Cullere, M., Biasato, I., Biasibetti, E., et al. (2018). Black soldier fly larva fat inclusion in finisher broiler chicken diet as an alternative fat source. Animal 12, 2032–2039. doi: 10.1017/S1751731117003743
Sedgh-Gooya, S., Torki, M., Darbemamieh, M., Khamisabadi, H., Karimi Torshizi, M. A., and Abdolmohamadi, A. (2021). Yellow mealworm, Tenebrio molitor (Col: Tenebrionidae), larvae powder as dietary protein sources for broiler chickens: Effects on growth performance, carcass traits, selected intestinal microbiota and blood parameters. J. Anim. Physiol. Anim. Nutr. (Berl.) 105, 119–128. doi: 10.1111/jpn.13434
Shang, Y., Kumar, S., Thippareddi, H., and Kim, W. K. (2018). Effect of dietary fructooligosaccharide (FOS) supplementation on ileal microbiota in broiler chickens. Poult. Sci. 97, 3622–3634. doi: 10.3382/ps/pey131
Shi, J., Zhao, D., Song, S., Zhang, M., Zamaratskaia, G., Xu, X., et al. (2020). High-meat-protein high-fat diet induced dysbiosis of gut microbiota and tryptophan metabolism in wistar rats. J. Agric. Food Chem. 68, 6333–6346. doi: 10.1021/acs.jafc.0c00245
Smetana, S., Schmitt, E., and Mathys, A. (2019). Sustainable use of Hermetia illucens insect biomass for feed and food: attributional and consequential life cycle assessment. Resour. Conserv. Recycl. 144, 285–296. doi: 10.1016/j.resconrec.2019.01.042
Wang, A. H., and Hogan, N. S. (2019). Performance effects of feed-borne Fusarium mycotoxins on broiler chickens: Influences of timing and duration of exposure. Anim. Nutr. 5, 32–40. doi: 10.1016/j.aninu.2018.08.001
Wang, J., Fan, H., Han, Y., Wei, J. P., Zhao, J. Z., and Zhou, Z. J. (2017). Pyrosequencing of the broiler chicken gastrointestinal tract reveals the regional similarity and dissimilarity of microbial community. Can. J. Anim. Sci. 97, 302–313. doi: 10.1139/cjas-2015-0120
Wang, T., Shen, Q., Feng, W., Wang, C., and Yang, F. (2020). Aqueous ethyl acetate as a novel solvent for the degreasing of black soldier fly (Hermetia illucens L.) larvae: degreasing rate, nutritional value evaluation of the degreased meal, and thermal properties. J. Sci. Food Agric. 100, 1204–1212. doi: 10.1002/jsfa.10131
Wang, Y. S., and Shelomi, M. (2017). Review of black soldier fly (Hermetia illucens) as animal feed and human food. Foods 6:91. doi: 10.3390/foods6100091
Weththasinghe, P., Lagos, L., Cortes, M., Hansen, J. O., and Overland, M. (2021). Dietary inclusion of black soldier fly (Hermetia illucens) larvae meal and paste improved gut health but had minor effects on skin mucus proteome and immune response in Atlantic salmon (Salmo salar). Front. Immunol. 12:599530. doi: 10.3389/fimmu.2021.599530
Xia, Y., Wang, M., Gao, F., Lu, M., and Chen, G. (2020). Effects of dietary probiotic supplementation on the growth, gut health and disease resistance of juvenile Nile tilapia (Oreochromis niloticus). Anim. Nutr. 6, 69–79. doi: 10.1016/j.aninu.2019.07.002
Xie, Y., Liu, J., Wang, H., Luo, J., Chen, T., Xi, Q., et al. (2020). Effects of fermented feeds and ginseng polysaccharides on the intestinal morphology and microbiota composition of Xuefeng black-bone chicken. PLoS One 15:e0237357. doi: 10.1371/journal.pone.0237357
Yadav, S., and Jha, R. (2019). Strategies to modulate the intestinal microbiota and their effects on nutrient utilization, performance, and health of poultry. J. Anim. Sci. Biotechnol. 10:2. doi: 10.1186/s40104-018-0310-9
Yu, M., Li, Z., Chen, W., Wang, G., Rong, T., Liu, Z., et al. (2020). Hermetia illucens larvae as a fishmeal replacement alters intestinal specific bacterial populations and immune homeostasis in weanling piglets. J. Anim. Sci. 98:skz395. doi: 10.1093/jas/skz395
Yue, S., Zhao, D., Peng, C., Tan, C., Wang, Q., and Gong, J. (2019). Effects of theabrownin on serum metabolites and gut microbiome in rats with a high-sugar diet. Food Funct. 10, 7063–7080. doi: 10.1039/c9fo01334b
Zhang, S., Zhong, G., Shao, D., Wang, Q., Hu, Y., Wu, T., et al. (2021). Dietary supplementation with Bacillus subtilis promotes growth performance of broilers by altering the dominant microbial community. Poult. Sci. 100:100935. doi: 10.1016/j.psj.2020.12.032
Zhao, J., Zhang, X., Liu, H., Brown, M. A., and Qiao, S. (2019). Dietary protein and gut microbiota composition and function. Curr. Protein Pept. Sci. 20, 145–154. doi: 10.2174/1389203719666180514145437
Keywords: Hermetia illucens larvae meal, Xuefeng black-boned chicken, intestinal morphology, intestinal microbial diversity, gut health
Citation: He C, Lei J, Yao Y, Qu X, Chen J, Xie K, Wang X, Yi Q, Xiao B, Guo S and Zou X (2021) Black Soldier Fly (Hermetia illucens) Larvae Meal Modulates Intestinal Morphology and Microbiota in Xuefeng Black-Bone Chickens. Front. Microbiol. 12:706424. doi: 10.3389/fmicb.2021.706424
Received: 07 May 2021; Accepted: 26 July 2021;
Published: 16 September 2021.
Edited by:
Jia Yin, Hunan Normal University, ChinaReviewed by:
Vahid Rezaeipour, Qaemshahr Islamic Azad University, IranRejun Fang, Hunan Agricultural University, China
Kai Qiu, Chinese Academy of Agricultural Sciences (CAAS), China
Copyright © 2021 He, Lei, Yao, Qu, Chen, Xie, Wang, Yi, Xiao, Guo and Zou. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Songchang Guo, Z3VvX2FzdEAxMjYuY29t; orcid.org/0000-0001-6067-3475; Xiaoyan Zou, ZGF5YW4yMjAwQDE2My5jb20=; orcid.org/0000-0003-1590-4179