Ancestral Sequence Reconstructions of MotB Are Proton-Motile and Require MotA for Motility
- 1School of Biotechnology and Biomolecular Sciences (BABS), University of New South Wales, Sydney, NSW, Australia
- 2School of Biological Sciences, University of Auckland, Auckland, New Zealand
- 3CSIRO Synthetic Biology Future Science Platform, Brisbane, QLD, Australia
A Corrigendum on
Ancestral Sequence Reconstructions of MotB Are Proton-Motile and Require MotA for Motility
by Islam, M. I., Lin, A., Lai, Y.-W., Matzke, N. J., and Baker, M. A. B. (2020). Front. Microbiol. 11:625837. doi: 10.3389/fmicb.2020.625837
In the original article, in the Introduction, paragraph four, we referenced previous work on ancestral reconstruction. We would like to add a further sentence and three additional citations for the work that first demonstrated the resurrection of ancient genes into contemporary hosts to form ancient modern hybrids and examine function in vivo:
It is possible to then resurrect the ancestral proteins corresponding to these inferred sequences and characterize their in vitro biological and biochemical properties (Gaucher et al., 2003; Thornton et al., 2003). Furthermore, ancient proteins can be expressed in contemporary hosts to examine the function of ancient proteins in vivo, and their integration and subsequent adaptation in ancient-modern hybrids (Kaçar and Gaucher, 2012; Kacar et al., 2017a,b).
Whilst our work does not explore subsequent adaptation of these ancient-modern hybrids, citation of these references is appropriate to give appropriate credit and to guide the reader to consider the research in ancestral reconstruction that has enabled our work.
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
References
Gaucher, E. A., Thomson, J. M., Burgan, M. F., and Benner, S. A. (2003). Inferring the palaeoenvironment of ancient bacteria on the basis of resurrected proteins. Nature 425, 285–288. doi: 10.1038/nature01977
Kacar, B., Garmendia, E., Tuncbag, N., Andersson, D. I., and Hughes, D. (2017a). Functional constraints on replacing an essential gene with its ancient and modern homologs. mBio 8:e01276-17. doi: 10.1128/mBio.01276-17
Kaçar, B., and Gaucher, E. (2012). “Towards the recapitulation of ancient history in the laboratory: combining synthetic biology with experimental evolution,” in Artificial Life 13: Proceedings of the 13th International Conference on the Simulation and Synthesis of Living Systems, ALIFE 2012 (East Lansing, MI), 11–18.
Kacar, B., Ge, X., Sanyal, S., and Gaucher, E. A. (2017b). Experimental evolution of Escherichia coli harboring an ancient translation protein. J. Mol. Evol. 84, 69–84. doi: 10.1007/s00239-017-9781-0
Keywords: motility, flagellar and chemotaxis, stator, ancestral sequence reconstruction, ion-selectivity
Citation: Islam MI, Lin A, Lai Y-W, Matzke NJ and Baker MAB (2021) Corrigendum: Ancestral Sequence Reconstructions of MotB Are Proton-Motile and Require MotA for Motility. Front. Microbiol. 12:650373. doi: 10.3389/fmicb.2021.650373
Received: 07 January 2021; Accepted: 11 February 2021;
Published: 19 March 2021.
Edited and reviewed by: Masahiro Ito, Toyo University, Japan
Copyright © 2021 Islam, Lin, Lai, Matzke and Baker. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Matthew A. B. Baker, matthew.baker@unsw.edu.au