CORRECTION article

Front. Microbiol., 30 October 2020

Sec. Infectious Agents and Disease

Volume 11 - 2020 | https://doi.org/10.3389/fmicb.2020.599031

Corrigendum: Comprehensive Molecular Characterization of Escherichia coli Isolates from Urine Samples of Hospitalized Patients in Rio de Janeiro, Brazil

  • 1. Departamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ciências Médicas, Universidade Do Estado Do Rio de Janeiro, Rio de Janeiro, Brazil

  • 2. Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Groningen, Netherlands

  • 3. Departamento de Controle de Infecções, Hospital Rio Laranjeiras, Rio de Janeiro, Brazil

  • 4. Departamento de Doenças Infecciosas e Parasitárias, Universidade Federal Do Estado Do Rio de Janeiro, Rio de Janeiro, Brazil

  • 5. Departamento de Doenças Infecciosas e Parasitárias, Universidade Do Estado Do Rio de Janeiro, Rio de Janeiro, Brazil

In the original article, there was a mistake in Table 2 and Supplementary Data Sheet 1, S.6 as published. The virotype of the isolate 4233 is not typeable. The corrected Table 2 and Supplementary Data Sheet 1, S.6 appear below.

Table 2

IsolatesPhylogenetic groupFimH typeSerotypeVirotypeESBL genesFluoroquinolone resistanta
5332B2fimH22O25:H4DblaCMY−2Pos
7018B2fimH30O25:H4AblaOXA−1Pos
7104B2fimH30O25:H4C2blaKPC−2Pos
9260B2fimH30O25:H4CblaCTX−M−15Pos
3218B2fimH30O25:H4C2blaKPC−2Pos
9581AB2fimH30O25:H4CblaCTX−M−15Pos
x5770dB2fimH30O25:H4CblaCTX−M−15Pos
x6638B2fimH30O25:H4AblaCTX−M−15Pos
1294DB2fimH30O25:H4BblaKPC−2Pos
2102B2fimH30O25:H4AblaKPC−2Pos
1710DB2fimH30O25:H4CblaCTX−M−15Pos
9533DB2fimH30O25:H4CblaCTX−M−15Pos
3528B2fimH30O25:H4C2blaCTX−M−15Neg
7078B2fimH30O25:H4C3blaTEM−1BPos
9893B2fimH30O25:H4C2blaKPC−2Neg
7974B2fimH30O25:H4DblaCTX−M−2Pos
4233B2fimH30O25:H4NTblaKPC−2Pos
5420B2fimH30O25:H4AblaCTX−M−15Neg
2478B2fimH41O16:H5AblaTEM−1BNeg
4006B2fimH41O16:H5AblaTEM−1BPos
5976B2fimH30O25:H4C3blaTEM−1BPos
2206B2fimH30O25:H4AblaCTX−M−15Pos
8565B2fimH30O25:H4C3blaTEM−1BPos
x2724B2fimH30O25:H4C2blaTEM−1BPos
6202B2fimH30O25:H4C2blaTEM−1BPos
5848B2fimH22O25:H4DblaCMY−2Neg

Distribution of fimH types among ST131 Escherichia coli isolates.

a

Neg. indicates susceptible to fluoroquinolones and Pos. indicates resistant to fluoroquinolones. NT, not typeable.

Supplementary data S.6

IsolatesFimH typeSerotypeafa/draBCafaoperon FM955459iroNsatibeApapG IIpapG IIIcnf1hlyAcdtBneuC-K1kpsM II-K2kpsM II-K5Virotype
5332fimH22O25:H4negnegposnegposnegnegnegnegnegposposnegD
7018fimH30O25:H4posposnegposnegnegnegnegnegnegnegposnegA
7104fimH30O25:H4negnegnegposnegnegnegnegnegnegnegnegposC2
9260fimH30O25:H4negnegnegposnegposnegposnegnegnegposnegC
3218fimH30O25:H4negnegnegposnegnegnegnegnegnegnegnegposC2
9581AfimH30O25:H4negnegnegposnegposnegposnegnegnegposnegC
x5770dfimH30O25:H4negnegnegposnegposnegposnegnegnegposnegC
x6638fimH30O25:H4posposnegposnegnegnegnegnegnegnegposnegA
1294DfimH30O25:H4negnegposposnegnegnegnegnegnegnegnegposB
2102fimH30O25:H4posposnegposnegnegnegnegnegnegnegposnegA
1710DfimH30O25:H4negnegnegposnegposnegposnegnegnegnegposC
9533DfimH30O25:H4negnegnegposnegposnegposnegnegnegposnegC
3528fimH30O25:H4negnegnegposnegnegnegnegnegnegnegnegposC2
7078fimH30O25:H4negnegnegposnegnegnegnegnegnegnegnegnegC3
9893fimH30O25:H4negnegnegposnegnegnegnegnegnegnegnegposC2
7974fimH30O25:H4negnegnegnegposnegnegnegnegnegnegposnegD
4233fimH30O25:H4negnegnegnegnegnegnegnegnegnegnegnegnegNT
5420fimH30O25:H4posposnegposnegnegnegnegnegnegnegposnegA
2478fimH41O16:H5posposnegnegnegnegnegnegnegnegnegposnegA
4006fimH41O16:H5posposnegnegnegnegnegnegnegnegnegposnegA
5976fimH30O25:H4negnegnegposnegnegnegnegnegnegnegnegnegC3
2206fimH30O25:H4posposnegposnegnegnegnegnegnegnegposnegA
8565fimH30O25:H4negnegnegposnegnegnegnegnegnegnegnegnegC3
x2724fimH30O25:H4negnegnegposnegnegnegnegnegnegnegnegposC2
6202fimH30O25:H4negnegnegposnegnegnegnegnegnegnegnegposC2
5848fimH22O25:H4negnegposnegposnegnegnegnegnegposposnegD

Virotypes distribution in ST131 isolates.

pos (positive) and neg (negative); Some isolates were'nt possible to subtyping according with Dahbi et al., 2014 were just typing according Blanco et al., 2014, and other were not possible to type being classified as NT, not typeable.

In the original article, there was an error in the presented percentage of the virotype D among ST131 isolates.

A correction has been made to Results, Escherichia coli ST131, Paragraph 1

UPEC strains produce different adhesins and fimbriae, including type 1 fimbriae. The FimH protein is the adhesive subunit of type 1 fimbriae that is used for epidemiological typing of UPEC. In this study, three fimH types were identified among the ST131 isolates, two O25:H4/ST131 isolates belonged to fimH22, two O16:H5/ST131 isolates to fimH41 while the majority of O25:H4/ST131 isolates (n = 22) belonged to fimH30 (Table 2). The virulence genes (afa/draBC, iroN, sat, ibeA, papGII, papGIII, cnf-1, hlyA, cdtB, neuC-K1, kpsMIIK2, kpsmII-K5) were used to determine the virotype of ST131 isolates based on the virulence profile. O25:H4/ST131 isolates belonged to different virotypes, i.e., 7 (26.92%) to virotype A, 1 (3.84%) to virotype B, 14 (53.84%) to virotype C, and 3 (11.53%) to virotype D. Isolates belonging to virotype C could be divided into subtypes C2 (n = 6) or C3 (n = 3), whereas five isolates could not be further subtyped. The only two isolates with serotype O16:H5/ST131 were classified as virotype A (see Data Sheet S6). Almost all O25:H4/ST131 isolates were resistant to fluoroquinolones, whereas the O16:H5/ST131 isolates were susceptible to this antibiotic. The blaCTX−M gene was most prevalent in O25:H4/ST131 fimH30 fluoroquinolones resistant (O25:H4/ST131-H30-R) isolates belonging to virotype C. Within ST131, blaCTX−M15 was confined to the H30-R sub-clone known as O25:H4/ST131-H30-Rx, represented by 9 (34.61%) isolates (Table 2).

The authors apologize for these errors and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

Summary

Keywords

Escherichia coli, urinary tract infections, Brazil, ST131, antibiotic resistance, virulence genes, whole genome sequencing, diagnostic stewardship

Citation

Campos ACC, Andrade NL, Ferdous M, Chlebowicz MA, Santos CC, Correal JCD, Lo Ten Foe JR, Rosa ACP, Damasco PV, Friedrich AW and Rossen JWA (2020) Corrigendum: Comprehensive Molecular Characterization of Escherichia coli Isolates from Urine Samples of Hospitalized Patients in Rio de Janeiro, Brazil. Front. Microbiol. 11:599031. doi: 10.3389/fmicb.2020.599031

Received

26 August 2020

Accepted

15 September 2020

Published

30 October 2020

Volume

11 - 2020

Edited and reviewed by

Jorge Blanco, University of Santiago de Compostela, Spain

Updates

Copyright

*Correspondence: John W. A. Rossen

This article was submitted to Infectious Diseases, a section of the journal Frontiers in Microbiology

Disclaimer

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

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