Weichao Ren
Na Liu2†
Qianqian Yang
Changjun Chen- 1College of Plant Protection, Nanjing Agricultural University, Nanjing, China
- 2Institute of Biotechnology, Zhejiang University, Hangzhou, China
- 3College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, China
- 4Plant Protection Station of Pizhou City, Xuzhou, China
High-osmolarity glycerol (HOG) signaling pathway belongs to mitogen-activated protein kinase (MAPK) cascades that regulate responses of organism to diverse extracellular stimuli. The membrane spanning proteins Sho1 and Sln1 serve as biosensors of HOG pathway in Saccharomyces cerevisiae. In this study, we investigated the biological functions of BcSHO1 and BcSLN1 in the gray mold fungus Botrytis cinerea. Target gene deletion demonstrated that both BcSHO1 and BcSLN1 are important for mycelial growth, conidiation and sclerotial formation. The BcSHO1 and BcSLN1 double deletion mutant ΔBcSln1-Sho1 produced much more, but smaller sclerotia than ΔBcSho1 and the wild-type (WT) strain, while ΔBcSln1 failed to develop sclerotia on all tested media, instead, formed a large number of conidia. Infection tests revealed that the virulence of ΔBcSln1-Sho1 decreased significantly, however, ΔBcSho1 or ΔBcSln1 showed no difference with the WT strain. In addition, ΔBcSln1-Sho1 exhibited resistance to osmotic stress by negatively regulating the phosphorylation of BcSak1 (yeast Hog1). All the phenotypic defects of mutants were recovered by target gene complementation. These results suggest that BcSHO1 and BcSLN1 share some functional redundancy in the regulation of fungal development, pathogenesis and osmotic stress response in B. cinerea.
Introduction
Botrytis cinerea (teleomorph: Botryotinia fuckeliana) is a devastating fungal pathogen that causes gray mold on over 500 plant species worldwide (Fillinger and Elad, 2016). Moreover, B. cinerea infects crop hosts during both pre- and post-harvesting phases and leads to huge economic losses (Dean et al., 2012). Currently, because of the lack of resistant varieties, chemical control is still the most effective strategy for controlling gray mold, however, many kinds of fungicides have lost effectiveness due to resistance development (Hu et al., 2016; Weber and Hahn, 2019). Therefore, exploring the molecular mechanisms underlying vegetative differentiation, pathogenesis and stress response in B. cinerea will contribute to establish more efficient disease management strategies.
Cells sense and respond to a variety of extracellular signals through ubiquitous mitogen-activated protein kinase (MAPK) cascades (Pearson et al., 2001). To date, five MAPK pathways have been identified in the eukaryotic model Saccharomyces cerevisiae, which regulate diverse physiological processes, including filamentation, sporulation, osmoregulation, cell wall integrity and pheromone response (Gustin et al., 1998). Osmoregulation is mainly controlled by the (high-osmolarity glycerol) HOG signaling pathway to adapt external hyperosmotic stress (Hohmann et al., 2007). Specifically, signals from the Sho1 and Sln1 branches are independently transduced by unique components and converge to activate the MAPK kinase (MAPKK) Pbs2. Cdc42, Ste20, and Ste50 are essential for Sho1 branch to activate the MAPK kinase kinase (MAPKKK) Ste11 (Saito and Tatebayashi, 2004). The Sln1 branch activates two partially redundant MAPKKs, Ssk2, and Ssk22 through Ypd1 and Ssk1 to Reiser et al. (2003), Pbs2 could be activated by any of the three osmoresponsive MAPKKKs (Ste11, Ssk2, or Ssk22), then the activated Pbs2 phosphorylates the MAPK Hog1, resulting in the translocation of Hog1 to nucleus and finally regulates the expression of responsible genes (Figure 1A; O’Rourke and Herskowitz, 2003).
Figure 1. Model of the yeast hyperosmotic-response MAPK pathway. (A) The osmotic signals from Sho1 or Sln1 are transduced by unique components and converge to activate Pbs2. The Sho1 branch requires Cdc42, Ste20, and Ste50 to activate Ste11. The Sln1 protein activates Ssk2 and Ssk22 through Ypd1 and Ssk1. Any of Ste11, Ssk2, or Ssk22 is able to activate Pbs2, which then phosphorylates Hog1, resulting in translocation of Hog1 to the nucleus that regulates responsible genes expression for osmoadaptation. (B) The schematic diagram of Sho1 and Sln1 orthologs in Botrytis cinerea. The functional domains were retrieved in EnsemblFungi (https://fungi.ensembl.org/Botrytis_cinerea/Info/Index). (C) Subcellular localization of BcSho1-GFP and BcSln1-GFP fusion proteins in B. cienrea. Bars, 10 μm.
The HOG pathway also involved in oxidative and cell wall stress response, through the interaction with the cell wall integrity (CWI) pathway (Brewster et al., 1993; Schüller et al., 1994). In addition to the conserved function of osmoregulation, the HOG pathway also plays several species-specific roles (Jiang et al., 2018). For example, the HOG pathway is dispensable for pathogenesis in some pathogenic fungi, such as Magnaporthe oryzae, Bipolaris oryzae, and Colletotrichum orbiculare (Li et al., 2012; Turrà et al., 2014), however, mutants blocked in this pathway exhibit severe defect in host infection in Candida albicans, Fusarium graminearum, Zymoseptoria tritici, Verticillium dahlia, and Cochliobolus sativus (Jiang et al., 2018). In phytopathogenic fungi, the HOG pathway also plays species-specific roles in growth and development, such as the regulation of conidiation and perithecium formation in F. graminearum and microsclerotium formation in V. dahliae (Zheng et al., 2012; Wang et al., 2016). Nevertheless, in general, the HOG pathway play crucial roles in oxidative stress responses and tolerance to phenylpyrrole and dicarboximide fungicides, despite its function in response to cell wall and other stresses may vary among different fungi (Jiang et al., 2018).
There are several core elements of HOG pathway in B. cinerea, including the osmosensor histidine kinase BcOs1, the histidine phosphotransfer protein BcHpt1, two response regulators BcRrg1 and BcSkn7, the MAPKKK BcOs4, the MAPKK BcOs5 and the MAPK BcSak1 (Viefhues et al., 2015). Up to now, most of the core proteins have been well characterized and showed that the HOG pathway plays unique roles in vegetative differentiation and stress responses in B. cinerea (Viaud et al., 2006; Segmüller et al., 2007; Liu et al., 2008; Yan et al., 2010; Yang et al., 2012). To further understand the function of HOG pathway in B. cinerea, we identified and investigated the upstream sensor proteins BcSho1 and BcSln1 in this pathway, and demonstrated the roles in fungal development, virulence and stress responses.
Materials and Methods
Fungal Strains and Culture Conditions
Strain B05.10 of B. cinerea was used as a recipient strain for the transformation experiments and as a wild-type control. The WT, resultant gene deletion and complemented strains were grown at 25 C on potato dextrose agar (PDA), complete medium (CM) and minimal medium (MM) for mycelial growth tests (Ren et al., 2018). B. cinerea strains were incubated on PDA plates under white light for conidiation or in the darkness for sclerotial formation. Sensitivity tests to osmotic stress were performed on PDA plates containing NaCl (1.5 M), KCl (1.5 M) or Sorbitol (2 M). The inhibition ratio of mycelial growth was calculated as a percentage of colony radial growth on medium with inhibitor compared with that on normal medium. Each experiment was repeated three times independently.
Gene Deletion and Complementation
Generation of the gene deletion and complementation strains were performed with the protocol described previously (Ren et al., 2017). To obtain BcSHO1 and BcSLN1 double deletion mutant, BcSHO1 was knocked out from BcSLN1 single deletion mutant. The primers used to amplify gene fragments were listed in Supplementary Table S1. Putative gene deletion mutants were identified by PCR and further confirmed by southern blotting analyses.
Construction of GFP Fusion Cassettes and Microscopy
To construct BcSho1-GFP fusion cassette, the open-reading fragment (without stop codon) of BcSHO1 was amplified and assembled with the NcoI-digested plasmid pNAN-OGG (Schumacher, 2012) using a One Step Cloning Kit (Vazyme Biotech, Nanjing, China). Using the same strategy, BcSln1-GFP cassette was also constructed. The resulting recombinant vectors were sequenced to ensure accuracy of the in-frame fusion region, and then transformed into the corresponding deletion mutants. The resultant transformants were screened by PCR and fluorescence signal. Subcellular localization was observed with a confocal laser scanning microscope (Leica TCS SP8, Germany).
Pathogenicity Assays
Primary leaves of strawberry were point-inoculated with the mycelial plugs of 3-day-old cultures. Prior to inoculation, the leaves were wounded with a sterilized needle to facilitate the penetration of plant tissues. Apple and grape fruits were harvested at the maturity stage, and were also wounded with a sterilized needle before inoculation. The detached host samples were moisturized and incubated at 25 C with 16 h of daylight. Lesion diameters were measured at 3 days (leaves) or 4 days (fruits) post-incubation using the crossing method. Three samples were incubated for each strain, and the experiments were repeated three times independently.
Sensitivity Tests to Different Fungicides
Mycelial plugs were taken from the colony margin and placed on the center of PDA plates amended with fludioxonil (0, 0.00625, 0.0125, 0.025, 0.05, and 0.1 μg/ml) or procymidone (0, 0.0625, 0.125, 0.25, 0.5, and 1 μg/ml) or iprodione (0, 0.125, 0.25, 0.5, 1, and 2 μg/ml). Three replicates per concentration for each strain. After incubation for 4 days at 25 C, the colony diameter was measured perpendicularly. EC50 (the effective concentration for 50% inhibition of mycelial growth) was calculated by the regression of the probit of the percentage of inhibition of radial growth against the logarithmic value of fungicide concentration. The experiments were repeated three times independently.
Determination of Intracellular Glycerol Content
Strains were grown in liquid YEPD (1% yeast extract, 2% peptone, and 2% dextrose) for 48 h and then treated with NaCl (0.8 M) for 30 min. The mycelia were harvested and ground in liquid nitrogen. 100 mg of mycelial powder were added to 1 ml of glycerol extraction buffer (Applygen Technologies Inc., Beijing, China), homogenized and centrifuged at 5000 g at for 20 min at 4 C. 5 μl supernatant of each sample was mixed with 195 μl of glycerol detection buffer (Applygen Technologies Inc.) in 96-well plate. The glycerol concentration was determined using an automated microplate reader (Bio-Rad, Japan) at 550 nm after the mixture had been incubated at 37 C for 20 min. The experiments were repeated three times independently.
Protein Manipulation and Western Blotting
Protein extraction was performed as described previously (Yu et al., 2014). The total proteins were separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to Immobilon-P transfer membrane (Millipore, Billerica, MA, United States). Phosphorylation of BcSak1 was detected by using an antibody against dually phosphorylated p38 (Thr180/Tyr182) (Cell Signaling Technology, Beverly, MA, United States). In addition, the samples were detected with anti-Hog1 antibody (C-terminal anti-Hog1) (Santa Cruz Biotechnology, Santa Cruz, CA, United States) as a reference. The experiments were repeated three times independently.
Results
Identification of BcSHO1 and BcSLN1 in B. cinerea
The Sho1 and Sln1 orthologs of B. cinerea were identified by BLASTP search of the B. cinerea genome database1 using S. cerevisiae Sho1 and Sln1 as queries, respectively. BcSHO1 (Gene ID: Bcin08p05150) is predicted to encode a 312 amino-acid protein with 32% identity to the transmembrane osmosensor Sho1 of S. cerevisiae. Protein domain analysis by SMART2 revealed that BcSho1 contains four characteristic transmembrane domains and a C-terminal SH3 domain. BcSLN1 (Gene ID: Bcin14p01870) is predicted to encode an 1184 amino-acid protein with 38% identity to the transmembrane histidine kinase and osmosensor Sln1 of S. cerevisiae. BcSln1 contains three characteristic transmembrane domains, a SH3 domain, a HAMP domain, a HisKA domain, a HATPase-c domain and a REC domain (Figure 1B). Subcellular localization showed that BcSho1-GFP and BcSln1-GFP fusion proteins were mainly located close to the cell membrane of mycelia in B. cinerea in some type of vesicular patches (Figure 1C).
Deletion of BcSHO1 and BcSLN1 in B. cinerea
To investigate the functions of BcSHO1 and BcSLN1 in B. cinerea, we generated both single and double deletion mutants ΔBcSho1, ΔBcSln1, and ΔBcSln1-Sho1, using homologous recombination strategy (Supplementary Figures S1A,C). The single deletion mutants were verified by Southern blotting analysis (Supplementary Figures S1B,D). To confirm that the phenotypic changes of mutants were due to gene deletion, the mutants were complemented with the full-length of BcSHO1 or BcSLN1, respectively, and generated the complemented strains ΔBcSho1-C and ΔBcSln1-C.
BcSHO1 and BcSLN1 Are Involved in Vegetative Growth and Conidiation
ΔBcSho1 and ΔBcSln1 showed similar growth rates to the WT strain B05.10 on PDA, but grew slower than B05.10 on either CM or MM especially ΔBcSln1. ΔBcSln1-Sho1 showed significantly reduced radial growth on all media tested compared with that of B05.10 (Figures 2A,C). Moreover, ΔBcSho1 and ΔBcSln1 formed denser colonies, in contrast, ΔBcSln1-Sho1 formed sparser colony with reduced aerial hyphae (Figure 2A). Microscopic examination showed that the hyphae of ΔBcSho1 and ΔBcSln1 beared more short lateral branches especially ΔBcSln1 produced shorter hyphae (Figure 2B).
Figure 2. Roles of BcSHO1 and BcSLN1 in vegetative growth. (A) Colony morphology of the wild-type B05.10, the single deletion mutants ΔBcSho1 and ΔBcSln1, the double deletion mutant ΔBcSln1-Sho1, and the complemented mutants ΔBcSho1-C and ΔBcSln1-C on PDA, CM and MM media at 25°C for 4 days. (B) Hyphal morphology of each strain growing on PDA plates. Bar, 100 μm (C) Colony diameters were measured in each independent biological experiment and statistically analyzed by Duncan analysis. Bars represent standard errors from three replications. Values on the bars followed by the same letter are not significantly different at P = 0.05.
Conidia serve as the primary inoculum for disease cycle of B. cinerea (Williamson et al., 2007). After incubated on PDA for 10 days, the amounts of conidia produced by ΔBcSho1, ΔBcSln1, and ΔBcSln1-Sho1 were significantly less than that of B05.10 (Figures 3A,C). Moreover, the conidia of ΔBcSho1, ΔBcSln1, and ΔBcSln1-Sho1 showed morphological abnormality and shifts in size (Figures 3B,D). These results indicate that both BcSHO1 and BcSLN1 play important roles in vegetative growth and conidiation of B. cinerea.
Figure 3. Roles of BcSHO1 and BcSLN1 in conidiation. (A) Comparison of conidiation among B05.10, ΔBcSho1, ΔBcSln1, and ΔBcSln1-Sho1 after 10 days incubation on PDA plates at 20°C under white light. (B) Conidial morphology of the each strain. Scale bar 10 μm. (C) The number of conidia produced by each strain. Bars represent standard errors from three replications. Values on the bars followed by the same letter are not significantly different at P = 0.05. (D) Conidial size of the each strain. More than 100 conidia were measured under a microscope in each experiment.
BcSHO1 and BcSLN1 Are Involved in Sclerotial Formation, Morphogenesis and Germination
Sclerotia represent an important survival mechanism in B. cinerea (Williamson et al., 2007), we therefore investigated the impacts of BcSHO1 and BcSLN1 on sclerotial formation. After incubation for 4 weeks, B05.10, ΔBcSho1, and ΔBcSln1-Sho1 formed sclerotia on all tested media, even though the size of sclerotia produced by ΔBcSho1 and ΔBcSln1-Sho1 were smaller than that of B05.10. Moreover, ΔBcSho1 produced less sclerotia on all tested media, and ΔBcSln1-Sho1 produced more sclerotia on PDA and CM, but less sclerotia on MM than that of B05.10. Notably, ΔBcSln1 failed to develop any sclerotia on all tested media, but produced a large number of conidia (Figures 4A–C), suggesting that BcSLN1 as such is not required for conidiation, but for its correct regulation under light vs. dark conditions. In addition, the sclerotial germination of ΔBcSho1 and ΔBcSln1-Sho1 was severely delayed compared with that of B05.10 (Figure 4D). These results indicate that BcSLN1 is essential for sclerotial development in B. cinerea, while BcSHO1 is less important, and there exists cooperative regulation between them.
Figure 4. Roles of BcSHO1 and BcSLN1 in sclerotial formation. (A) Colony morphology of sclerotial formation. B05.10, ΔBcSho1, ΔBcSln1, ΔBcSln1-Sho1, ΔBcSho1-C, and ΔBcSln1-C were cultured on PDA, CM and MM media at 4°C for 4 weeks in darkness. (B) The number of sclerotia produced by each strain on PDA, CM, and MM media. ND, not detected. Bars represent standard errors from three replications. Values on the bars followed by the same letter are not significantly different at P = 0.05. (C) The size of sclerotia produced by each strain. Bars represent standard errors from three replications. Values on the bars followed by the same letter are not significantly different at P = 0.05. (D) Sclerotial germination of each strain on PDA plates at 25°C at 1 and 2 days post-incubation (d.p.i.).
BcSHO1 and BcSLN1 Are Redundantly Required for Virulence
To determine the roles of BcSHO1 and BcSLN1 in virulence, infection tests were performed on different plant tissues. As shown in Figure 5, both ΔBcSho1 and ΔBcSln1 showed similar pathogenicity to the WT strain B05.10 on strawberry leaves and apple and grape fruits, however, the virulence of ΔBcSln1-Sho1 reduced significantly, suggesting that there is functional redundancy between BcSHO1 and BcSLN1 in terms of pathogenesis.
Figure 5. Roles of BcSHO1 and BcSLN1 in virulence. Infection tests on different plant tissues following incubation with B05.10, ΔBcSho1, ΔBcSln1, ΔBcSln1-Sho1, ΔBcSho1-C, and ΔBcSln1-C. Agar plugs without fungal mycelia were used as negative controls (CK). (A) Disease symptom on strawberry leaves at 3 days post-incubation (d.p.i.). (B) Disease symptom on apple fruits at 4 d.p.i. (C) Disease symptom on grape fruits at 4 d.p.i. (D) The diameter of disease lesions caused by each strain. Bars represent standard errors from three replications. Values on the bars followed by the same letter are not significantly different at P = 0.05.
BcSHO1 and BcSLN1 Redundantly Regulate Osmotic Stress Tolerance
Sho1 and Sln1 serve as the osmosensors of HOG pathway in yeast (Rispail et al., 2009), so we were interested in the impacts of BcSHO1 and BcSLN1 on osmotic stress tolerance in B. cinerea. As shown in Figure 6, ΔBcSln1-Sho1 showed significantly reduced sensitivity to osmotic stress generated by NaCl, KCl or sorbitol compared with that of B05.10, however, the sensitivity of ΔBcSho1 and ΔBcSln1 to osmotic stress remained unchanged.
Figure 6. Sensitivity of B05.10, ΔBcSho1, ΔBcSln1, and ΔBcSln1-Sho1 to osmotic stress. (A) All strains were grown on PDA plates amended with NaCl, KCl, or D-sorbitol at the indicated concentrations at 25°C for 4 days. (B) Statistical analysis of the growth inhibition rate of all strains under osmotic stress. Bars represent standard errors from three replications. Values on the bars followed by the same letter are not significantly different at P = 0.05.
Since phenylpyrrole and dicarboximide fungicides have been reported to activate HOG pathway in some fungal pathogens (Kojima et al., 2004), we tested the sensitivity of ΔBcSho1, ΔBcSln1, and ΔBcSln1-Sho1 to the phenylpyrrole fungicide, fludioxonil and the dicarboximide fungicides, procymidone and iprodione. ΔBcSho1 showed slightly reduced sensitivity (two-fold) to all tested fungicides, and ΔBcSln1-Sho1 only showed reduced sensitivity to fludioxonil, whereas the sensitivity of ΔBcSln1 to all tested fungicides remained unchanged (Table 1). These results indicate that BcSHO1 and BcSLN1 play redundant roles in osmotic stress response in B. cinerea.
Table 1. Sensitivity of the wild-type strain B05.10, the BcSHO1 deletion mutant ΔBcSho1, the BcSLN1 deletion mutant ΔBcSln1, and the BcSHO1 and BcSLN1 double deletion mutant ΔBcSln1-Sho1 to different fungicides.
BcSHO1 and BcSLN1 Function Redundantly to Negatively Regulate Glycerol Production and Hog1 Phosphorylation
The HOG pathway maintains osmotic homeostasis by Hog1 phosphorylation which regulates glycerol production (Burg et al., 1996), we therefore examined glycerol content and the phosphorylation of BcSak1 (yeast Hog1). Under normal conditions, the glycerol contents of ΔBcSho1, ΔBcSln1, and ΔBcSln1-Sho1 were similar to that of B05.10, but when treated with NaCl, ΔBcSln1-Sho1 showed significantly high glycerol production (Figure 7A).
Figure 7. Glycerol content and BcSak1 phosphorylation of B05.10, ΔBcSho1, ΔBcSln1, and ΔBcSln1-Sho1. (A) The amounts of glycerol (per g mycelial dry weight) produced by each strain in YEPD medium amended with 0.8 M NaCl. The cultures without any treatment were used as control (CK). Bars represent standard errors from three replications. Values on the bars followed by the same letter are not significantly different at P = 0.05. (B) The BcSak1 and phosphorylated BcSak1 proteins were detected using the yeast anti-Hog1p (C-terminal anti-Hog1) and phosphorylated p38 (Thr180/Tyr182) antibodies, respectively.
Phosphorylation analysis revealed that the phosphorylation level of BcSak1 in ΔBcSln1-Sho1 was significantly increased under hyperosmotic conditions compared with that of B05.10, ΔBcSho1 and ΔBcSln1 (Figure 7B), which was consistent with the above results. These results indicate that BcSHO1 share redundancy with BcSLN1 in regulating the response of B. cinerea to external osmotic stress.
Discussion
Living cells are equipped with precise mechanisms for sensing external signal, such as osmosis, oxidation, and hormones, which allow adaptation to environment through a variety of cellular responses triggered by sensing and subsequent signaling systems (Bahn et al., 2007). In this study, we characterized the roles of two sensor proteins of HOG pathway in B. cinerea and found that the single or double deletion mutants of BcSHO1 and BcSLN1 exhibited severe defects in growth, conidium and sclerotium formation, however, only the BcSHO1 and BcSLN1 double deletion mutant showed a significant reduction in virulence. In addition, single or double deletion of BcSHO1 and BcSLN1 made no difference to oxidative and cell wall stress sensitivity (data not shown), and only the double deletion increased the phosphorylation level of BcSak1 (an ortholog of S. cerevisiae Hog1), which in return resulted in tolerance to osmotic stresses. In S. cerevisiae, the Sho1 and Sln1 branches are redundant for promoting growth on high-osmolarity condition, but they also display some functional specificities (O’Rourke and Herskowitz, 2003).
Both Sho1 and Sln1 proteins in budding yeast serve as biosensors to response to extracellular environmental stimuli (O’Rourke et al., 2002). In this study, compared with the WT strain, single deletion of BcSHO1 or BcSLN1 in B. cinerea exhibited similar sensitivity to osmotic and oxidative stress and virulence suggesting that the roles of BcSHO1 and BcSLN1 are dramatically different from that of S. cerevisiae. Likewise, as the major sensing proteins in pathogenic fungi, the hybrid histidine-kinase (HK) of class III are generally implicated in oxidant condition adaptation, dicarboximide and phenylpyrrole antifungal sensitivity, cell wall integrity, and conidiation, however, it still remains the species-specific roles (Defosse et al., 2015; Hérivaux et al., 2016).
Pathogenicity experiment revealed that the double deletion mutant of BcSHO1 and BcSLN1 caused the decreased virulence, suggesting that BcSho1 and BcSln1 have an additive effect on pathogenicity of B. cinerea. However, the disease severity of ΔBcSln1-Sho1 was dramatically less than that of ΔFgSln1-Sho1 in F. graminearum (Gu et al., 2014), indicating that the involvement of the BcSln1 and BcSho1 branches in pathogenesis is less important than that in F. graminearum.
Previous study in F. graminearum showed that disruption of FgSHO1 results in a reduction in sporulation, whereas the deletion of FgSLN1 displays an undetectable change in sporulation (Gu et al., 2014). MoSho1 and MoSln1 are required for sporulation in M. oryzae (Zhang et al., 2010; Liu et al., 2011), and deletion of tcsA (a homolog of Sln1 in S. cerevisiae) attenuated sporulation in A. nidulans (Furukawa et al., 2002). In the current study, both BcSHO1 and BcSLN1 showed dramatically decrease in sporulation, suggesting that the effect of Sln1 on sporulation differs in fungi. In addition, the spore sizes from the mutants and WT strain differed considerably. Spores in statistical size from smallest to biggest were ranked as an order of ΔBcSln1 < ΔBcSho1 < WT strain (B05.10) < ΔBcSln1-Sho1. To our knowledge, this is first reported that the roles of BcSHO1 and BcSLN1 are involved in the size of spore.
Sclerotia are asexual and resting structures of some agricultural crop pathogens, including Claviceps purpurea, Sclerotinia sclerotiorum, and B. cinerea (Coley-Smith and Cooke, 1971). Disruption of BcSak1 in B. cinerea causes increased number of sclerotia (Segmüller et al., 2007). In this study, ΔBcSln1, not ΔBcSho1, failed to form sclerotia, while the double deletion of BcSHO1 and BcSLN1 resulted in increased sclerotial development on rich medium. Notably, ΔBcSln1 produced a large number of conidia instead of sclerotia in the darkness. For Botrytis, vegetative mycelia differentiate conidiophores and conidia when incubated in the light and sclerotia in constant darkness (Schumacher, 2017). Therefore, BcSLN1 participates in the light-dependent development of B. cinerea. Moreover, the procedure of sclerotia formation mainly includes initiation, development and maturation, and three groups of factors are reportedly involved in sclerotial formation, including environmental signals, Ser/Thr phosphatase type 2A and 2B, cellular sensing and signal transduction (Giesbert et al., 2011). BcSho1 and BcSln1 branches may play a role in sensing and signal transduction for sclerotial formation in B. cinerea.
Besides osmotic stress, HOG pathway is also involved in cellular responses to oxidative, ultraviolet radiation, heavy metal, and heat stress in fungi (Irniger et al., 2016). In Aspergillus nidulans, the SakA (yeast Hog1) mutants showed more sensitive to osmotic and oxidative stress, and the phosphorylated SakA undergoes nuclear import under osmotic and oxidative stress conditions (Jaimes-Arroyo et al., 2015). In Neurospora crassa, the Os2 (yeast Hog1) mutants are highly sensitive to high osmolarity and exhibit resistance to fungicides such as phenylpyrrole (Zhang et al., 2002). The FgOS-2 deletion mutants are sensitive to osmotic stress but not to oxidative stress or fungicides in F. graminearum (Nguyen et al., 2012). In this study, the double-gene deletion mutant ΔBcSln1-Sho1 exhibited increased tolerance to osmotic stress by BcSak1 phosphorylation, indicating that an extra pathway may be triggered and/or strengthened to activate the HOG pathway. In S. cerevisiae, the HOG pathway perceives the osmotic stress signals from the five transmembrane osmosensors, including Sho1, Sln1, Msb1, Hkr1, and Opy2 (Maeda et al., 1994). Opy2 reportedly serves as a third osmosensor in the Sho1 branch that anchors ste50 and ste11 in budding yeast (Yamamoto et al., 2010). According to the data from an online platform of KEGG3, B. cinerea MAPK-HOG pathway harbors three transmembrane osmosensors, i.e., BcSln1, BcSho1 and BcOpy2 (Gene code: BCIN_04g03130 in B. cinerea B05.10). We therefore postulated that the MAPK-HOG branch [Opy2-Ste50-Ste11-Pbs2-Sak1(Hog1)] is triggered at least on the condition of the double disruption of BcSHO1 and BcSLN1 in B. cinerea. Additionally, only ΔBcSho1 displayed stable resistance against phenylpyrrole and dicarboximide fungicides tested, while deletion of Sln1, not Sho1 in C. neoformans, A. nidulans, and A. fumigatus exhibited resistance against phenylpyrrole or/and dicarboximide fungicides (Chen et al., 2012), suggesting that the unique effect of BcSHO1 on the resistance against these fungicides maybe results from the specific branch [Opy2-Ste50-Ste11-Pbs2-Sak1(Hog1)]. Detailed characterization of this branch and their orchestration with other MAPK-HOG signaling cascades is under scrutiny.
Author Contributions
WR, CC, and QG conceived and designed the experiments. WR, NL, and YY performed the experiments, analyzed the data, prepared figures and tables. WR, QY, and CC wrote and revised the paper. All authors have read and approved the final manuscript.
Funding
This work was supported by the National Natural Science Foundation of China (Nos. 31672065 and 31801778), Chinese National Key R&D Plans (Nos. 2016YED0201007 and 2018YFD0201201), and Jiangsu Provincial Agricultural Plans [Nos. CX(18)2005 and PZCZ201715].
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Supplementary Material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2019.00328/full#supplementary-material
FIGURE S1 | Generation and identification of BcSHO1 and BcSLN1 deletion mutants. (A) Gene replacement strategy of BcSHO1. (B) Southern blotting analysis of BcSHO1 mutant. (C) Gene replacement strategy of BcSLN1. (D) Southern blotting analysis of BcSLN1 mutant.
TABLE S1 | Oligonucleotide primers used in this study.
Footnotes
- ^http://fungi.ensembl.org/Botrytis_cinerea/Info/Index
- ^http://smart.embl-heidelberg.de/
- ^https://www.kegg.jp/
References
Bahn, Y. S., Xue, C., Idnurm, A., Rutherford, J. C., Heitman, J., and Cardenas, M. E. (2007). Sensing the environment: lessons from fungi. Nat. Rev. Microbiol. 5, 57–69. doi: 10.1038/nrmicro1578
Brewster, J. L., de Valoir, T., Dwyer, N. D., Winter, E., and Gustin, M. C. (1993). An osmosensing signal transduction pathway in yeast. Science 259, 1760–1763. doi: 10.1126/science.7681220
Burg, M. B., Kwon, E. D., and Kültz, D. (1996). Osmotic regulation of gene expression. FASEB J. 10, 1598–1606. doi: 10.1096/fasebj.10.14.9002551
Chen, L. H., Lin, C. H., and Chung, K. R. (2012). Roles for SKN7 response regulator in stress resistance, conidiation and virulence in the citrus pathogen Alternaria alternata. Fungal Genet. Biol. 49, 802–813. doi: 10.1016/j.fgb.2012.07.006
Coley-Smith, J. R., and Cooke, R. C. (1971). Survival and germination of fungal sclerotia. Annu. Rev. Phytopathol. 9, 65–92. doi: 10.1146/annurev.py.09.090171.000433
Dean, R., van Kan Jan, A. L., Pretorius Zacharias, A., Hammond-Kosack Kim, E., Di Pietro, A., Spanu Pietro, D., et al. (2012). The top 10 fungal pathogens in molecular plant pathology. Mol. Plant Pathol. 13, 414–430. doi: 10.1111/j.1364-3703.2011.00783.x
Defosse, T. A., Sharma, A., Mondal, A. K., Dugé de Bernonville, T., Latgé, J. P., Calderone, R., et al. (2015). Hybrid histidine kinases in pathogenic fungi. Mol. Microbiol. 95, 914–924. doi: 10.1111/mmi.12911
Fillinger, S., and Elad, Y. (eds) (2016). Botrytis - the fungus, the pathogen and its management in agricultural systems. New York, NY: Springer, doi: 10.1007/978-3-319-23371-0
Furukawa, K., Katsuno, Y., Urao, T., Yabe, T., Yamada-Okabe, T., Yamada-Okabe, H., et al. (2002). Isolation and functional analysis of a gene, tcsB, encoding a transmembrane hybrid-type histidine kinase from Aspergillus nidulans. Appl. Environ. Microb. 68, 5304–5310. doi: 10.1128/AEM.68.11.5304-5310.2002
Giesbert, S., Schumacher, J., Kupas, V., Espino, J., Segmüller, N., Haeuser-Hahn, I., et al. (2011). Identification of pathogenesis-associated genes by T-DNA-mediated insertional mutagenesis in Botrytis cinerea: a type 2A phosphoprotein phosphatase and an SPT3 transcription factor have significant impact on virulence. Mol. Plant Microbe Interact. 25, 481–495. doi: 10.1094/MPMI-07-11-0199
Gu, Q., Chen, Y., Liu, Y., Zhang, C., and Ma, Z. (2014). The transmembrane protein FgSho1 regulates fungal development and pathogenicity via the MAPK module Ste50-Ste11-Ste7 in Fusarium graminearum. New Phytol. 206, 315–328. doi: 10.1111/nph.13158
Gustin, M. C., Albertyn, J., Alexander, M., and Davenport, K. (1998). MAP kinase pathways in the yeast Saccharomyces cerevisiae. Microbiol. Mol. Biol. R. 62, 1264–1300.
Hérivaux, A., So, Y. S., Gastebois, A., Latgé, J. P., Bouchara, J. P., Bahn, Y. S., et al. (2016). Major sensing proteins in pathogenic fungi: the hybrid histidine kinase family. PLoS Pathog. 12:e1005683. doi: 10.1371/journal.ppat.1005683
Hohmann, S., Krantz, M., and Nordlander, B. (2007). Chapter two - yeast osmoregulation. Method. Enzymol. 428, 29–45. doi: 10.1016/S0076-6879(07)28002-4
Hu, M. J., Cox, K. D., and Schnabel, G. (2016). Resistance to increasing chemical classes of fungicides by virtue of “selection by association” in Botrytis cinerea. Phytopathology 106, 1513–1520. doi: 10.1094/PHYTO-04-16-0161-R
Irniger, S., Sarikaya-Bayram,Ö, and Bayram, Ö (2016). “Fungal MAP-Kinase-mediated regulatory pathways,” in Biochemistry and Molecular Biology, ed. D. Hoffmeister (Cham: Springer international publishing), 97–117. doi: 10.1007/978-3-319-27790-5_6
Jaimes-Arroyo, R., Lara-Rojas, F., Bayram,Ö, Valerius, O., Braus, G. H., and Aguirre, J. (2015). The SrkA kinase is part of the SakA mitogen-activated protein kinase interactome and regulates stress responses and development in Aspergillus nidulans. Eukaryot. Cell. 14, 495–510. doi: 10.1128/EC.00277-14
Jiang, C., Zhang, X., Liu, H., and Xu, J. R. (2018). Mitogen-activated protein kinase signaling in plant pathogenic fungi. PLoS Pathog. 14:e1006875. doi: 10.1371/journal.ppat.1006875
Kojima, K., Takano, Y., Yoshimi, A., Tanaka, C., Kikuchi, T., and Okuno, T. (2004). Fungicide activity through activation of a fungal signalling pathway. Mol. Microbiol. 53, 1785–1796. doi: 10.1111/j.1365-2958.2004.04244.x
Li, G., Zhou, X., and Xu, J. R. (2012). Genetic control of infection-related development in Magnaporthe oryzae. Curr. Opin. Microbiol. 15, 678–684. doi: 10.1016/j.mib.2012.09.004
Liu, W., Leroux, P., and Fillinger, S. (2008). The HOG1-like MAP kinase Sak1 of Botrytis cinerea is negatively regulated by the upstream histidine kinase Bos1 and is not involved in dicarboximide- and phenylpyrrole-resistance. Fungal Genet. Biol. 45, 1062–1074. doi: 10.1016/j.fgb.2008.04.003
Liu, W., Zhou, X., Li, G., Li, L., Kong, L., Wang, C., et al. (2011). Multiple plant surface signals are sensed by different mechanisms in the rice blast fungus for appressorium formation. PLoS Pathog. 7:e1001261. doi: 10.1371/journal.ppat.1001261
Maeda, T., Wurgler-Murphy, S. M., and Saito, H. (1994). A two-component system that regulates an osmosensing MAP kinase cascade in yeast. Nature 369, 242–245. doi: 10.1038/369242a0
Nguyen, T. V., Schäfer, W., and Bormann, J. (2012). The stress-activated protein kinase FgOS-2 is a key regulator in the life cycle of the cereal pathogen Fusarium graminearum. Mol. Plant Microbe Interact. 25, 1142–1156. doi: 10.1094/MPMI-02-12-0047-R
O’Rourke, S. M., and Herskowitz, I. (2003). Unique and redundant roles for HOG MAPK pathway components as revealed by whole-genome expression analysis. Mol. Biol. Cell. 15, 532–542. doi: 10.1091/mbc.e03-07-0521
O’Rourke, S. M., Herskowitz, I., and O’Shea, E. K. (2002). Yeast go the whole HOG for the hyperosmotic response. Trends Genet. 18, 405–412. doi: 10.1016/S0168-9525(02)02723-3
Pearson, G., Robinson, F., Beers Gibson, T., Xu, B., Karandikar, M., Berman, K., et al. (2001). Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions. Endocr. Rev. 22, 153–183. doi: 10.1210/edrv.22.2.0428
Reiser, V., Raitt, D. C., and Saito, H. (2003). Yeast osmosensor Sln1 and plant cytokinin receptor Cre1 respond to changes in turgor pressure. J. Cell Biol. 161:1035. doi: 10.1083/jcb.200301099
Ren, W., Liu, N., Sang, C., Shi, D., Zhou, M., Chen, C., et al. (2018). The autophagy gene BcATG8 regulates the vegetative differentiation and pathogenicity of Botrytis cinerea. Appl. Environ. Microb. 84:e2455-17. doi: 10.1128/AEM.02455-17
Ren, W., Zhang, Z., Shao, W., Yang, Y., Zhou, M., and Chen, C. (2017). The autophagy-related gene BcATG1 is involved in fungal development and pathogenesis in Botrytis cinerea. Mol. Plant Pathol. 18, 238–248. doi: 10.1111/mpp.12396
Rispail, N., Soanes, D. M., Ant, C., Czajkowski, R., Grünler, A., Huguet, R., et al. (2009). Comparative genomics of MAP kinase and calcium-calcineurin signalling components in plant and human pathogenic fungi. Fungal Genet. Biol. 46, 287–298. doi: 10.1016/j.fgb.2009.01.002
Saito, H., and Tatebayashi, K. (2004). Regulation of the osmoregulatory HOG MAPK cascade in yeast. J. Biochem. 136, 267–272. doi: 10.1093/jb/mvh135
Schüller, C., Brewster, J. L., Alexander, M. R., Gustin, M. C., and Ruis, H. (1994). The HOG pathway controls osmotic regulation of transcription via the stress response element (STRE) of the Saccharomyces cerevisiae CTT1 gene. EMBO J. 13, 4382–4389. doi: 10.1002/j.1460-2075.1994.tb06758.x
Schumacher, J. (2012). Tools for Botrytis cinerea: new expression vectors make the gray mold fungus more accessible to cell biology approaches. Fungal Genet. Biol. 49, 483–497. doi: 10.1016/j.fgb.2012.03.005
Schumacher, J. (2017). How light affects the life of Botrytis. Fungal Genet. Biol. 106, 26–41. doi: 10.1016/j.fgb.2017.06.002
Segmüller, N., Ellendorf, U., Tudzynski, B., and Tudzynski, P. (2007). BcSAK1, a stress-activated mitogen-activated protein kinase, is involved in vegetative differentiation and pathogenicity in Botrytis cinerea. Eukaryot. Cell. 6, 211–221. doi: 10.1128/EC.00153-06
Turrà, D., Segorbe, D., and Di Pietro, A. (2014). Protein kinases in plant-pathogenic fungi: conserved regulators of infection. Annu. Rev. Phytopathol. 52, 267–288. doi: 10.1146/annurev-phyto-102313-050143
Viaud, M., Fillinger, S., Liu, W., Polepalli, J. S., Le Pêcheur, P., Kunduru, A. R., et al. (2006). A class III histidine kinase acts as a novel virulence factor in Botrytis cinerea. Mol. Plant Microbe Interact. 19, 1042–1050. doi: 10.1094/MPMI-19-1042
Viefhues, A., Schlathoelter, I., Simon, A., Viaud, M., and Tudzynski, P. (2015). Unraveling the function of the response regulator BcSkn7 in the stress signaling network of Botrytis cinerea. Eukaryot. Cell 14, 636–651. doi: 10.1128/EC.00043-15
Wang, Y., Tian, L., Xiong, D., Klosterman, S. J., Xiao, S., and Tian, C. (2016). The mitogen-activated protein kinase gene, VdHog1, regulates osmotic stress response, microsclerotia formation and virulence in Verticillium dahliae. Fungal Genet. Biol. 88, 13–23. doi: 10.1016/j.fgb.2016.01.011
Weber, R. W. S., and Hahn, M. (2019). Grey mould disease of strawberry in northern Germany: causal agents, fungicide resistance and management strategies. Appl. Microbiol. Biot. doi: 10.1007/s00253-018-09590-1 [Epub ahead of print].
Williamson, B., Tudzynski, B., Tudzynski, P., and van Kan Jan, A. L. (2007). Botrytis cinerea: the cause of grey mould disease. Mol. Plant Pathol. 8, 561–580. doi: 10.1111/j.1364-3703.2007.00417.x
Yamamoto, K., Tatebayashi, K., Tanaka, K., and Saito, H. (2010). Dynamic control of yeast MAP kinase network by induced association and dissociation between the Ste50 scaffold and the Opy2 membrane anchor. Mol. Cell. 40, 87–98. doi: 10.1016/j.molcel.2010.09.011
Yan, L., Yang, Q., Sundin, G. W., Li, H., and Ma, Z. (2010). The mitogen-activated protein kinase kinase BOS5 is involved in regulating vegetative differentiation and virulence in Botrytis cinerea. Fungal Genet. Biol. 47, 753–760. doi: 10.1016/j.fgb.2010.06.002
Yang, Q., Yan, L., Gu, Q., and Ma, Z. (2012). The mitogen-activated protein kinase kinase kinase BcOs4 is required for vegetative differentiation and pathogenicity in Botrytis cinerea. Appl. Microbiol. Biotechnol. 96, 481–492. doi: 10.1007/s00253-012-4029-9
Yu, F., Gu, Q., Yun, Y., Yin, Y., Xu, J. R., Shim, W. B., et al. (2014). The TOR signaling pathway regulates vegetative development and virulence in Fusarium graminearum. New Phytol. 203, 219–232. doi: 10.1111/nph.12776
Zhang, H., Liu, K., Zhang, X., Song, W., Zhao, Q., Dong, Y., et al. (2010). A two-component histidine kinase, MoSLN1, is required for cell wall integrity and pathogenicity of the rice blast fungus, Magnaporthe oryzae. Curr. Genet. 56, 517–528. doi: 10.1007/s00294-010-0319-x
Zhang, Y., Lamm, R., Pillonel, C., Lam, S., and Xu, J. R. (2002). Osmoregulation and fungicide resistance: the Neurospora crassa os-2 gene encodes a HOG1 mitogen-activated protein kinase homologue. Appl. Environ. Microb. 68, 532–538. doi: 10.1128/AEM.68.2.532-538.2002
Keywords: high-osmolarity glycerol pathway, osmosensor, development, virulence, osmotic stress
Citation: Ren W, Liu N, Yang Y, Yang Q, Chen C and Gao Q (2019) The Sensor Proteins BcSho1 and BcSln1 Are Involved in, Though Not Essential to, Vegetative Differentiation, Pathogenicity and Osmotic Stress Tolerance in Botrytis cinerea. Front. Microbiol. 10:328. doi: 10.3389/fmicb.2019.00328
Received: 22 September 2018; Accepted: 08 February 2019;
Published: 25 February 2019.
Edited by:
Jerome Collemare, Westerdijk Fungal Biodiversity Institute, NetherlandsReviewed by:
Sabine Fillinger, Institut National de la Recherche Agronomique (INRA), FranceElvira Román, Complutense University of Madrid, Spain
Copyright © 2019 Ren, Liu, Yang, Yang, Chen and Gao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Changjun Chen, Y2hhbmdqdW4tY2hlbkBuamF1LmVkdS5jbg== Qingli Gao, Z3FsMzMzM0AxNjMuY29t
†These authors have contributed equally to this work as joint first authors