This article is a commentary on:
Diversity of reductive dehalogenase genes from environmental samples and enrichment cultures identified with degenerate primer PCR screens
Laura A. Hug
Elizabeth A. Edwards- 1Department of Cell and Systems Biology, University of Toronto, Toronto, ON, Canada
- 2Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada
A corrigendum on
Diversity of reductive dehalogenase genes from environmental samples and enrichment cultures identified with degenerate primer PCR screens
by Hug, L. A., and Edwards, E. A. (2013). Front. Microbiol. 4:341 doi: 10.3389/fmicb.2013.00341
The PCR amplification conditions were inaccurate as originally published, omitting the number of cycles for the final amplification step—a total of 25 cycles should be conducted when annealing at 50°C. The corrected methods are included below.
The following conditions were subsequently applied to all described samples: an initial denaturation at 95°C for 5 min, three cycles of denaturation at 95°C for 30 s, primer annealing at 38°C for 30 s, and elongation at 72°C for 90 s, three cycles of denaturation at 95°C for 30 s, primer annealing at 45°C for 30 s, and elongation at 72°C for 90 s, and 25 cycles of denaturation at 95°C for 30 s, primer annealing at 50°C for 30 s, and elongation at 72°C for 90 s, and a final extension at 72°C for 10 min.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Keywords: Erratum, Amplicon sequencing, diversity, reductive dehalogenase, organohalide respiration
Citation: Hug LA and Edwards EA (2015) Corrigendum: Diversity of reductive dehalogenase genes from environmental samples and enrichment cultures identified with degenerate primer PCR screens. Front. Microbiol. 6:30. doi: 10.3389/fmicb.2015.00030
Received: 22 December 2014; Accepted: 10 January 2015;
Published online: 03 February 2015.
Edited and reviewed by: Claudia Lüke, Radboud University Nijmegen, Netherlands
Copyright © 2015 Hug and Edwards. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: laura.hug@berkeley.edu
†Present address: Laura A. Hug, Department of Earth and Planetary Science, University of California, Berkeley, Berkeley, CA, USA