Junjie Wu
Zhengqi Chang*- Department of Orthopedics, 960th Hospital of PLA, Jinan, China
Purpose: To analyze the feasibility and effectiveness of bacterial culture in negative pressure wound drainage (NPWD) fluid in patients with Pyogenic Vertebral Osteomyelitis (PVO).
Methods: A retrospective analysis was performed on 17 patients with PVO who were treated with negative pressure drainage at the Department of Orthopedics in our hospital from January 1, 2018 to December 31, 2021. Data was obtained while the patients were in the hospital, including 12 males and 5 females with an average age of 57.71 ± 9.93 years. After applying the negative pressure drainage technique, the drainage fluid was collected and a bacterial culture was performed. The positive rate of bacterial culture in the drainage fluid was recorded. Comparing the positive rate of specimen culture obtained by this surgical method with other methods reported in the literature, the effectiveness of negative pressure drainage in the treatment of PVO was analyzed.
Results: All patients were placed with negative pressure sponge during operation and underwent continuous negative pressure aspiration after operation. The average of total drainage volume was 186.47 ± 29.44 mL. The drainage fluid was successfully retained for bacterial culture, and the results of bacterial culture were negative in 3 patients. Pathogenic bacteria were successfully obtained from negative pressure drainage fluid in 14 cases, with a positive rate of 82.4% (14/17).
Conclusion: Using negative pressure drainage to retain drainage fluid for bacterial culture can significantly increase the positive rate, which is helpful for the diagnosis of PVO and rational antibiotic treatment.
Introduction
Spinal infections are a common type of bone infection, accounting for approximately 3% of all bone infections (1). The prevalence of spinal infections is on the rise due to factors such as increased life expectancy, chronic illnesses, antibiotic usage, and spinal surgeries (2). In China, the aging population, improved diagnostic techniques, and heightened susceptibility have contributed to the increasing occurrence of spinal infections (3). Symptoms of spinal infections typically include low back pain, swelling, and other discomforts. If left untreated, these infections can result in nerve damage or even death (4). However, spinal infections are often misdiagnosed due to their subtle onset and atypical symptoms, leading to an average diagnosis time of 2–6 months (5). In cases where a spinal infection is confirmed, some patients may require surgical intervention, especially if they have nerve damage, spinal instability, or spinal deformities (6). Proper administration of antibiotics is essential for both conservative and surgical treatments, with the selection of antibiotics based on bacterial culture and drug sensitivity. For non-specific vertebral infections (Pyogenic Vertebral Osteomyelitis), the identification of pathogenic microorganisms through bacterial culture and the effective use of sensitive antibiotics are crucial priorities in clinical management.
Due to the low rate of positive blood culture results in patients with abscesses, puncturing to obtain pus for culture and identification of pathogenic bacteria is the most reliable method of diagnosis and treatment (7). However, primary spinal infections are located deep, with few skin sinus openings, and large abscesses tend to spread along the bilateral psoas major muscles, making it more difficult to obtain specimens than with other superficial infections. Common techniques for obtaining specimens include B-ultrasound or CT guided puncture and drainage surgery, intervertebral foramen endoscopic lesion clearance surgery, and incision lesion clearance surgery (8). Nevertheless, in some cases, the positive rate of pus culture is still not satisfactory due to the prolonged use of antibiotics.
We implemented Negative Pressure Wound Drainage (NPWD) technology to treat deep infection after spinal surgery, with successful results and a higher rate of bacterial culture (9). We then extended the use of NPWD to treat primary spinal infection, using small incisions to place negative pressure drainage for continuous drainage of secretions from patients with Pyogenic Vertebral Osteomyelitis (PVO). This method proved to be effective and safe, with a higher rate of bacterial culture and satisfactory clinical results when compared to other surgical methods reported in the literature.
Materials and methods
A retrospective study was carried out on 57 patients diagnosed with pyogenic vertebral osteomyelitis (PVO) at our clinic from January 2018 to December 2021. The study focused on individuals who underwent debridement surgery and subsequently received continuous negative pressure wound therapy. Seventeen PVO patients were included in the study, consisting of 12 males and 5 females, with an average age of 57.71 ± 9.93 years. The cases consisted of one thoracic infection, one combined thoracic and lumbar infection, six lumbar infections, four combined lumbar and psoas major muscle infections, two infections after posterior cervical internal fixation, and three infections after posterior lumbar internal fixation. Preoperative antibiotic use was recorded in 76.5% of cases. Negative pressure drainage technology was utilized, and drainage fluid was collected for bacterial culture to determine the positivity rate. Subsequent internal fixation surgery was considered based on infection control outcomes.
After administering general anesthesia, the surgical site is prepared by disinfecting and covering it with tissue. The procedure begins with the use of a percutaneous screw fixator, followed by a small incision to remove the affected area through the intervertebral foramen. Tissue samples are taken for bacterial culture and pathological examination. Physiological saline is used to flush out the infected area during the surgery, and an NPWD sponge is placed to complete the procedure (Figure 1). A negative pressure drainage bottle is replaced 24 h later to maintain sterility and avoid contamination, and the fluid is collected for bacterial culture. Bacterial culture is performed according to the National Clinical Laboratory Operating Regulations. Specimens are inoculated onto various agar plates and incubated in a carbon dioxide incubator at 35°C for 16–20 h. The bacteria are then isolated, purified, and identified using a fully automated microbiological analyzer (Figure 2).
Figure 1. A 68-year-old male was admitted to the hospital with a month-long history of low back pain and fever. (A) MRI revealed increased signals on the anterior and inferior margins of the L3 and L4 vertebrae, as well as T2 liposuppressive images in the L3/4 space. (B) The patient was then placed in a lateral position and underwent a small incision debridement. (C,D) A negative pressure drainage sponge was inserted into the intervertebral space, and the negative pressure was connected. (E) Intraoperative fluoroscopy showed that the sponge was in the correct position (marked in red box).
Figure 2. (A,B) The negative pressure drainage bottle and tube are replaced after 24 h, and the fluid is collected for bacterial culture. (C) Staphylococcus aureus is identified in the bacterial culture of the drainage fluid. (D) Staphylococcus aureus is confirmed by mass spectrometry, with an identification score of 2.29 (scores below 1.7 are not reliable, scores between 1.7 and 2.0 indicate possible genus-level identification, and scores equal to or greater than 2.0 indicate possible species-level identification).
Results
All patients had negative pressure sponges placed during surgery, followed by postoperative continuous negative pressure suction. The negative pressure drainage fluid was collected 24 h after surgery, and 5-10 mL was taken for bacterial culture. The total drainage volume was an average of 186.47 ± 29.44 mL. Out of the 17 patients, 14 (82.4%) had pathogenic bacteria in their drainage fluid, including 4 Staphylococcus aureus, 3 Staphylococcus epidermidis, 2 Streptococcus viridans, 1 Streptococcus pharyngitis, 1 Escherichia coli, 1 Enterobacter cloacae, 1 Klebsiella pneumoniae, and 1 Candida tropicalis. The remaining 3 patients had negative bacterial culture results (Table 1).
Table 1. shows general information of patients and bacterial culture results.
Discussion
In statistical analysis, the positive rate of bacterial culture from tissue samples taken during surgery was found to be 47.06% (8/17), which is consistent with previous studies (10–13). This indicates that our method of obtaining samples and conducting cultures is reliable, but also suggests that the positive rate of bacterial culture from intraoperative tissue samples is not very optimistic. In this study, our team innovatively applied NPWD technology to the treatment of spinal infections, resulting in a total drainage volume of 186.47 ± 29.44 mL, which is significantly higher than the drainage volume obtained from non-NPWD (14). Furthermore, the positive rate of bacterial culture was increased to 82.4%, which is a significant improvement. This is crucial because spinal infections have a poor prognosis, with a reported fatality rate of approximately 6% and a disability rate of around 27% (15, 16). If left untreated, this condition can have serious consequences for the patient’s life and lead to various complications (15, 16). Antibiotics are the main form of treatment for spinal infections, whether conservative or surgical methods are used (1, 17). However, in cases where sepsis is present or the cause of the infection is unknown, it is recommended to start empirical antibiotic treatment. The suggested duration of antibiotic treatment for bone tissue infections is 3–8 weeks, while for non-specific spinal infections, it is recommended to use antibiotics for at least 6 weeks (18). Even with such a long period of antibiotic treatment, the effectiveness may be compromised without bacterial culture and sensitivity results (19). Therefore, improving the positive rate of bacterial culture is crucial, and our research results are of great help in this regard.
Several factors can affect the likelihood of detecting microbial cultures, including prior antibiotic use, the amount of infected tissue obtained, specimen collection method, and sample storage. The probability of identifying pathogenic bacteria decreases significantly if antibiotics were used before sample collection, dropping from 70 to 30%. Therefore, it is recommended to identify the specific pathogenic microorganisms before starting antibiotic treatment if possible. While infections like spinal tuberculosis or brucellosis can be detected through serological tests, non-specific infections such as Staphylococcus aureus or Escherichia coli have a lower positivity rate in blood cultures, typically ranging from 40 to 60% (10). Pus cultures have a wider range of positivity rates, varying from 30 to 91% (11–13), but not all patients produce pus.
The traditional tissue culture method has a higher positive rate compared to blood culture, but it is still not satisfactory. Research has shown that the positive rate of specimens obtained through minimally invasive extraction, incision extraction, or ordinary drainage is similar (20). In our case data, the positive rate of tissue samples obtained during surgery and sent for bacterial culture was 47.06% (8/17), which is consistent with the literature. This could be due to the small amount of pathogenic bacteria present and the use of antibiotics by many patients before treatment. Furthermore, catheter drainage does not significantly increase the positive rate of drainage culture compared to other specimen cultures. In our study, a significant increase in the positive culture rate was observed. This could be attributed to the negative pressure generated by Negative Pressure Wound Therapy (NPWT), which continuously extracts tissue exudates, pus, and other substances. The pressure is evenly distributed on the surface of polyvinyl alcohol (PVA), creating comprehensive drainage. NPWT is able to remove water from bacterial biofilms and disrupt the bacterial growth environment (21). Additionally, NPWT can attract immune cells such as macrophages and white blood cells to the infected site, enhancing their activity and phagocytosis (22). With the continuous negative pressure effect, bacteria are drained into a collection bottle outside the body, meeting the cultivation requirements.
In conclusion, antibiotics are essential for treating spinal infections. The traditional method of blood culture and puncture specimen bacterial culture has a low success rate. However, using negative pressure drainage to collect drainage fluid for cultivation can significantly increase the positive rate and aid in the diagnosis and treatment of spinal infection. Despite this, this study was a single center retrospective study with a limited sample size, so further research with a larger sample size is necessary.
Data availability statement
The original contributions presented in the study are included in the article/supplementary material, further inquiries can be directed to the corresponding author.
Ethics statement
The studies involving humans were approved by Ethics Committee of the 960th hospital of PLA. The studies were conducted in accordance with the local legislation and institutional requirements. The participants provided their written informed consent to participate in this study. Written informed consent was obtained from the individual(s) for the publication of any potentially identifiable images or data included in this article.
Author contributions
JW: Data curation, Investigation, Writing – original draft. ZC: Conceptualization, Funding acquisition, Investigation, Resources, Writing – review & editing.
Funding
The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This research was supported by Natural Science Foundation of Shandong Province (Award Number: ZR2023MH331; Grant Recipient: Zhengqi Chang).
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
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Keywords: spinal infection, negative pressure drainage, bacterial culture, positive rate, drug sensitivity result
Citation: Wu J and Chang Z (2024) Investigating the feasibility and effectiveness of bacterial culture in negative pressure wound drainage fluid. Front. Med. 11:1479698. doi: 10.3389/fmed.2024.1479698
Edited by:
Débora Coraça-Huber, Innsbruck Medical University, AustriaReviewed by:
Yesholata Mahabeer, National Health Laboratory Service (NHLS), South AfricaZehua Zhang, Army Medical University, China
Ting Wang, The Affiliated Hospital of Qingdao University, China
Copyright © 2024 Wu and Chang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Zhengqi Chang, MjY3NjY3NzFAcXEuY29t