Circulating levels of mitochondrial oxidative stress-related peptides MOTS-c and Romo1 in stable COPD: A cross-sectional study

Background: MOTS-c and Romo1 are mitochondrial peptides that are modulated by oxidative stress. No previous studies have explored circulating levels of MOTS-c in patients with chronic obstructive pulmonary disease (COPD).

Methods: We enrolled 142 patients with stable COPD and 47 smokers with normal lung function in an observational cross-sectional study. We assessed serum levels of both MOTS-c and Romo1 and associated these findings with clinical characteristics of COPD.

Results: Compared with smokers with normal lung function, patients with COPD had lower levels of MOTS-c (p = 0.02) and higher levels of Romo1 (p = 0.01). A multivariate logistic regression analysis revealed that above-median MOTS-c levels were positively associated with Romo1 levels (OR 1.075, 95% CI 1.005–1.150, p = 0.036), but no association was found with other COPD characteristics. Below-median levels of circulating MOTS-c were associated with oxygen desaturation (OR 3.25 95% CI 1.456–8.522, p = 0.005) and walking <350 meters (OR 3.246 95% CI 1.229–8.577, p = 0.018) in six-minute walk test. Above-median levels of Romo1 were positively associated with current smoking (OR 2.756, 95% CI 1.133–6.704, p = 0.025) and negatively associated with baseline oxygen saturation (OR 0.776 95% CI 0.641–0.939, p = 0.009).

Conclusions: Reduced levels of circulating MOTS-c and increased levels of Romo1 were detected in patients diagnosed with COPD. Low levels of MOTS-c were associated with oxygen desaturation and poorer exercise capacity using 6 min walk test. Romo1 was associated with current smoking and baseline oxygen saturation.

Trial registration: www.clinicaltrials.gov; No.: NCT04449419; URL: www.clinicaltrials.gov. Date of registration: June 26, 2020.

Introduction

Chronic Obstructive Pulmonary Disease (COPD) is one of the leading causes of mortality worldwide (1). Oxidative stress (OS) is one of the most important factors contributing to the pathogenesis and severity of COPD (2, 3). COPD is a heterogeneous disease with several potentially treatable traits that have been associated with disease prognosis (4) including muscle weakness (5), reduced exercise capacity (6), and increased oxygen desaturation (OD) during exercise (7). While several biomarkers of OS have been evaluated in COPD (8, 9), none are evaluated on a routine basis.

Mitochondrial open reading frame of the 12S ribosomal RNA type-c (MOTS-c) is a recently discovered micropeptide encoded by the mitochondrial genome. MOTS-c is produced primarily in mitochondria-rich tissues, including skeletal muscle (10). MOTS-c has also been detected in peripheral blood and has thus been tentatively identified as a circulating myomitokine (11). While circulating MOTS-c penetrates target cells rapidly, its mechanism of entry remains unknown (11). Receptors for the hormonal mitokine, humanin, have already been described; by contrast, no receptors have been identified that interact specifically with MOTS-c. In vitro, MOTS-c is detected primarily in the mitochondria; production is induced in response to glucose restriction (10) or OS (12). MOTS-c activates sarcoplasmic adenosine monophosphate-activated protein kinase (AMPK) and is then translocated to the nucleus, where it binds to antioxidant response element sequences in the promoter regions of nuclear factor erythroid 2-related factor 2 (NRF2) and other transcription factors, thereby modulating target gene activity (12). Results of recent research reveal that circulating levels of MOTS-c increase with acute intense exercise but decline in association with increasing age as well as obesity, coronary disease, diabetes mellitus, and kidney failure (13).

Reactive oxygen species modulator 1 (Romo1) is a redox-sensitive protein located in the inner mitochondrial membrane that regulates the integrity of mitochondrial cristae and mitochondrial shape under conditions of OS (14). Of note, defective cristae, abnormal branches, and swollen and fragmented organelles are frequently observed in respiratory epithelial cells isolated from COPD patients (3).

To the best of our knowledge, there are no previously published studies that document serum MOTS-c levels in patients diagnosed with COPD or smokers without COPD. On the other hand, the results of one small study documented higher serum Romo1 levels in COPD patients (n = 49) compared to healthy volunteers not matched by smoking status (n = 39) and a negative correlation between Romo1 levels and forced expiratory volume in the first second (FEV1) (%), but exercise capacity was not evaluated (15). Although MOTS-c and Romo1 are mitochondrial peptides, and are associated with chronic diseases, they have not been measured together before.

We hypothesized that, because of low exercise capacity and high OS characteristic of COPD, serum levels of both MOTS-c and Romo1 would be altered. We also hypothesized that circulating levels might be associated with specific outcomes of this disease, for example, exercise capacity.

Methods

An observational cross-sectional study of patients receiving care at a COPD outpatient clinic in Spain was performed from November 2018 to December 2020. The study was reviewed and approved by the ethics committee of our institution (CEIm of Cantabria; 2018.276). Written informed consent was provided by all patients before entering the study. The study protocol was registered at www.clinicaltrials.gov (https://clinicaltrials.gov/ct2/show/NCT04449419).

Participants

Participants were recruited at a dedicated COPD outpatient clinic during routine visits. Smokers without COPD (control group) were recruited from the smoking cessation clinics held at our institution.

The inclusion criteria were as follows: (i) patients diagnosed with COPD based on the Global Initiative for Chronic Obstructive Lung Disease (GOLD) guidelines (16) who were >40 years of age and (ii) sex, age, and smoking status- matched control patients who had not been diagnosed with COPD.

The exclusion criteria were as follows: (i) patients who experienced a COPD exacerbation within 8 weeks of inclusion in the study; (ii) patients undergoing pulmonary rehabilitation during or up to 6 months before inclusion in the study; (iii) patients previously diagnosed with coronary or peripheral artery disease or cancer; (iv) patients with respiratory diseases different from COPD; (v) patients with serum C-reactive protein levels > 2.5 mg/dL; and (vi) patients with a glomerular filtration rate < 50 ml/min/1.73 m²; (vii) patients using systemic corticosteroids within 8 weeks of inclusion in the study.

Measurements

Body composition estimates were obtained using a bioelectrical impedance device (OMRON BF511, Omron, Japan). Spirometry and a six-minute walk test (6MWT) were performed according to the Spanish Society of Pneumology and Thoracic Surgery (SEPAR) protocol (17, 18). Maximum hand grip strength was measured with a GRIP-A hand dynamometer (Takei, Niigata, Japan). Disease-associated malnutrition was determined based on the European Society for Clinical Nutrition and Metabolism (ESPEN) consensus guidelines, including body mass index (BMI) <18.5 kg/m² or 18.5–22 kg/m², combined with a low fat-free mass index (FFMI) at <17 kg/m² for men and <15 kg/m² for women (19). Participants were categorized as having a high risk of exacerbation (HRE) at the time of entry into the study if they had two or more moderate or one severe COPD exacerbation during the previous year as per GOLD guidelines (17). Oxygen desaturation (OD) was defined as a fall in oxygen saturation (SpO₂) ≥4% or an overall SpO₂ <90% (20). Serum creatinine, albumin, uric acid, and creatine kinase levels were measured with IDMS-traceable enzymatic assays (Atellica^® Analyzer, Siemens, Germany).

Serum levels of MOTS-c were determined using sandwich immunoassay (Mitochondrial Open Reading Frame of the 12S rRNA-c: MOTS-c kit, CEX132Hu, Katy, Cloud-Clone Corp., TX, USA Lot number L210213153). Serum levels of Romo1 were determined using sandwich immunoassay (Reactive oxygen species modulator 1 (Human ROMO1) ELISA Kit, Elabscience^®, TX, USA Lot number ET4ZB9UKKQ) as per the manufacturers' protocols.

Blood from each participant was collected early in the morning to avoid any confusion that might result from circadian changes in MOTS-c and ROMO-1 levels. Samples and patient data were preserved by Biobanco Valdecilla (PT17/0015/0019), integrated into the Spanish Biobank Network, and processed according to standard operating procedures with approval from both ethical and scientific committees.

Statistical analysis

Normally distributed data are presented as means ± standard deviations (SDs). Non-parametric data are presented as medians with interquartile ranges. We calculated the sample size in Stata Statistical Software: Release 15. College Station, TX: StataCorp LLC) based on an α risk of 0.05 and a β risk of 0.2. Differences between groups were evaluated with unpaired t-tests (for parametric data) or Mann-Whitney tests (for non-parametric data). Normal distribution was determined using the Kolmogorov–Smirnov test. The creation of a dichotomized variable from MOTS-c and Romo1 levels with a cut-off at the median value resulted in the highest discriminative power for the outcomes under study as it resulted in the lowest Akaike information criterion value, similar to that observed in other studies (21, 22). We determined cross-sectional associations by univariate and multivariate logistic regression based on high (i.e., above the median) vs. low (i.e., below the median) values of baseline circulating MOTS-c and Romo1 with baseline characteristics of the patients. Using a similar model, we evaluated the primary outcomes of the study: baseline oxygen saturation, and two outcomes of the 6MWT [6 min walking distance (6MWD) and oxygen desaturation (OD)] using SPSS Software version 25.00 for PC. All p-values resulted from two-tailed tests with p < 0.05 as statistically significant.

Results

Characteristics of patients and controls

One hundred and forty-two COPD patients and forty-seven sex and age-matched controls (i.e., smokers who were not diagnosed with COPD) were enrolled in our study (Figure 1). Table 1 includes demographic, clinical, and biochemical data. The patient population was 66.2% men with a mean age of 67.5 ± 7.7 years. The COPD patient cohort included a substantial percentage of current smokers (30.6%); most had moderate to severe airway obstruction. The participants included in the control group had normal lung function, lower scores on COPD assessment tests (CATs), and were capable of longer six-minute walk distances (6MWD) than the COPD patients. As shown in Table 1 the percentage of current smokers was similar in COPD and control groups. The prevalence of type 2 diabetes mellitus (T2DM) which is a well-established cause of reductions in serum MOTS-c levels was similar in both groups (p = 0.842). Among our findings, MOTS-c levels were lower in the COPD group [median 622 ng/mL; interquartile range (IQR) 482–848 ng/mL] compared to controls (median 764 ng/mL; IQR 604–906 ng/mL; p = 0.022) (Figure 2). Our findings revealed no significant differences between serum levels of MOTS-c detected in patients with COPD either with (median 681 ng/mL, IQR 452–1150 ng/mL) or without T2DM (median 688 ng/mL, IQR 504–924 ng/mL; p = 0.853). Furthermore, serum levels of MOTS-c did not correlate with hemoglobin A1c (r = 0.165, p = 0.649) in patients with T2DM; thus, all COPD patients were evaluated as a single group.

FIGURE 1

Figure 1. Flowchart for patient selection.

TABLE 1

Table 1. Demographic, clinical, and biochemical characteristics of control (smokers without COPD) and COPD patients.

FIGURE 2

Figure 2. MOTS-c and Romo1 distribution in patients with COPD and smokers without COPD.

Serum levels of Romo1 were higher among those in the COPD group (median 5.42 ng/mL, IQR 2.84–8.72 ng/mL) vs. controls (median 3.72 ng/mL, IQR 1.64–7.59 ng/mL, p = 0.038) (Figure 2).

Association of circulating levels of MOTS-c and Romo1 with baseline characteristics of COPD

Table 2 highlights the associations of MOTS-c and Romo1 with baseline characteristics of COPD and between both peptides. Multivariate logistic regression analysis showed that high levels of MOTS-c were positively associated with Romo1 levels (OR 1.075, 95% CI 1.005–1.150, p = 0.036), but no association was found with other COPD characteristics. On the other hand, multivariate logistic regression analysis confirmed that high levels of Romo1 were independently and positively associated with current smoking (OR 2.756, 95% CI 1.133–6.704, p = 0.025) and circulating MOTS-c levels (OR 1.001, 95% CI 1.000–1.003, p = 0.012) (Table 2).

TABLE 2

Table 2. Univariate and multivariate logistic regression analysis for the associations between baseline chronic obstructive pulmonary disease characteristics and high levels of MOTS-c and Romo1.

Low MOTS-c and high Romo1 levels as predictors of basal oxygen saturation, distance and oxygen desaturation in 6MWT

Forty-eight patients with COPD presented with Oxygen desaturation (OD). Among these, 33 exhibited low MOTS-c levels and 23 patients exhibited high Romo1 levels. Thirty-five patients walked <350 m during the 6MWT; this included 25 patients with low MOTS-c levels and 14 patients with high Romo1 levels. Multivariate logistic regression (Table 3) showed that low MOTS levels were significantly associated with OD (OR 3.25 95% CI 1.456–8.522, p = 0.005) and walking <350 meters (OR 3.246 95% CI 1.229–8.577, p = 0.018), but not with baseline O₂ levels. On the other hand, high Romo 1 levels were negatively associated with baseline O₂ levels (OR 0.776 95% CI 0.641–0.939, p = 0.009), but not with OD or walking <350 m (Table 4).

TABLE 3

Table 3. Multivariate logistic regression analysis showing factors associated with baseline low MOTS-c.

TABLE 4

Table 4. Logistic regression analysis showing factors associated with baseline high Romo1.

Discussion

The results of our study provide the first evidence that circulating levels of MOTS-c are lower in patients with COPD compared with otherwise healthy current smokers; by contrast, circulating levels of Romo1 were higher in patients with COPD. MOTS-c and Romo1 were associated with different COPD characteristics; low circulating MOTS-c levels were associated with worse 6MWD and oxygen desaturation; by contrast, high circulating levels of Romo1 were associated with active smoking and lower baseline levels of oxygen saturation. Interestingly we found a positive association between Romo1 and MOTS-c.

Circulating MOTS-c levels were reduced in COPD patients. Similar responses have been observed in other chronic diseases associated with OS, including T2DM (23), obesity with or without obstructive sleep apnea syndrome (24, 25), endothelial dysfunction/coronary artery disease (26), kidney failure (27), and multiple sclerosis (28). Interestingly, concomitant T2DM resulted in no further reductions in circulating MOTS-c levels compared to patients with COPD alone; these results suggest that the effects of these two diseases on MOTS-c levels are not additive. Low MOTS-c levels, as happens with “low T3 sick euthyroid syndrome”, may be a generalized non-specific response to many illnesses (29).

Reductions in circulating MOTS-c levels may result from mitochondrial damage. Alternatively, OS may induce profound sequestration of MOTS-c in the nucleus, thereby blocking its transfer into the peripheral circulation (12). Given that low levels of circulating MOTS-c have been reported in numerous diseases associated with OS, the second explanation may be the more likely of the two.

Lower levels of circulating MOTS-c were associated with shorter distances walked in the 6MWT, specifically with walking < 350 m, a parameter that is associated with the risk of death in COPD (6) and more profound OD. Exercise induces MOTS-c synthesis in healthy individuals (13, 30); MOTS-c in turn activates the synthesis of other enzymes (such as AMP-activated protein kinase) known to be induced by exercise (11, 31). On the other hand, it has been reported recently that MOTS-c promotes muscle differentiation of muscle progenitor cells (32). Thus, it is reasonable to hypothesize that individuals who are unable to induce MOTS-c synthesis will exhibit a comparatively low exercise capacity. One recent study evaluated the relationship between oxidative biomarkers associated with stable COPD and their role in exercise, identifying superoxide dismutase (SOD) as an independent determinant of performance in the 6MWT (33).

Our study shows that patients diagnosed with COPD have higher circulating levels of Romo1. This finding was also suggested previously in a cross-sectional study that included a small population of COPD patients (15). The results of our study reveal for the first time an important association between high circulating levels of Romo1 with current smoking and reduced oxygen saturation (i.e., factors intrinsically associated with oxidative stress), although no relationship with exercise capacity. Our data revealed a positive (albeit weak) association between high circulating levels of MOTS-c and Romo1. This relationship that has not been described previously, warrants further research and contrasts with the fact that low MOTS-c is associated with worse outcomes related with exercise capacity but high Romo1 is associated with low oxygen saturation, suggesting that each molecule relates to different characteristics of COPD. In a different model of respiratory disease, Ye et al. (34) have recently reported that serum Romo1 and ROS were increased in patients with obstructive sleep apnea syndrome.

Several of the strengths of this study are worth highlighting. First, this study was designed specifically to evaluate the impact of COPD on circulating levels of MOTS-c and Romo1 in a group of carefully selected and well-characterized patients without comorbidities (other than T2DM and asymptomatic coronary diseases) that might influence the results and a matched for current smoking control group of smokers without COPD (in order to avoid smoking as a potential confounding factor). Second, we considered a variety of factors and clinical characteristics of COPD that might have an impact on circulating levels of MOTS-c and Romo1.

Our study has several limitations. Most importantly, this type of study reveals associations but not causality. Any assessments of causality will require specifically designed In vitro and in vivo experimental studies. Furthermore, largely because of the complex pathophysiology of mitochondrial dysfunction and oxidative stress, a full understanding of this phenomenon will require consideration of a large collection of markers. Finally, our patient cohort was enrolled from a single center. Thus, these findings will need to be replicated in large multicenter trials with patients from a large range of socio-demographic settings and who exhibit a variety of comorbidities that might influence the results.

Conclusion

Our study provides the first evidence of reductions in circulating levels of MOTS-c levels in patients diagnosed with COPD, lower MOTS-c is associated with lower 6MWD and higher rate of oxygen desaturation. Our study also revealed increases in levels of Romo1 in COPD patients that are associated with current smoking and baseline oxygen saturation but are not associated with 6MWD or oxygen desaturation in 6MWT. Further studies will be needed to confirm our findings which will open new perspectives in the multidimensional management of COPD.

Data availability statement

The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

Ethics statement

The studies involving human participants were reviewed and approved by the Ceim of Cantabria. The patients/participants provided their written informed consent to participate in this study.

Author contributions

Guarantor of the paper: CA. Conceptualization: CA and MG-U. Data curation: DF-P, CA, PM-A, and FM. Formal analysis: DF-P and CA. Project administration: CA and PM-A. Methodology: CA, MG-U, DB, AB, and AG. Resources: CA, MG-U, and AB. Visualization: CA and BL. Supervision: MG-U and PM-A. Software: DB. Writing—original draft: CA and CC. Writing—review and editing: CA, PM-A, AB, BL, AG, DB, and CC. All authors contributed to the article and approved the submitted version.

Funding

This study was funded by Instituto de Investigación Sanitaria of Cantabria (IDIVAL): NextVAL grant: NVAL19/01. This study received funding from GSK (NCT04449419). The funder was not involved in the study design, collection, analysis, interpretation of data, the writing of this article or the decision to submit it for publication. GSK was provided the opportunity to review a preliminary version of this manuscript for factual accuracy, but the authors are solely responsible for final content and interpretation.

Acknowledgments

We want to particularly acknowledge the patients and the Biobank Valdecilla (PT17/0015/0019) integrated in the Spanish Biobank Network for its collaboration.

Conflict of interest

CA has received speaker or consulting fees from Boehringer Ingelheim, Pfizer, AstraZeneca, Novartis, Chiesi, Faes Farma, Esteve, and GSK. CC has received speaker or consulting fees from AstraZeneca, Bial, Boehringer Ingelheim, Chiesi, GSK, Menarini, Novartis, and research grants from GSK, Menarini, and AstraZeneca. DF-P has received speaker or consulting fees from Chiesi and GSK. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher's note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Abbreviations

COPD, chronic obstructive pulmonary disease; MOTS-c, mitochondrial open reading frame of the 12S ribosomal RNA type-c; Romo1, reactive oxygen species modulator 1; 6MWD, six-minute walk distance; 6MWT, six-minute walk test; OD, oxygen desaturation; OS, oxidative stress; SEPAR, Spanish Society of Pneumology and Thoracic Surgery; BMI, body mass index; FFMI, fat-free mass index; HRE, high risk of exacerbation.

References

1. Lozano R, Naghavi M, Foreman K. Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analysis for the global burden of disease study 2010. Lancet. (2012) 380:2095–128.

PubMed Abstract | Google Scholar

2. Barnes PJ. Inflammatory mechanisms in patients with chronic obstructive pulmonary disease. J Allergy Clin Immunol. (2016) 138:16–27. doi: 10.1016/j.jaci.2016.05.011

PubMed Abstract | CrossRef Full Text | Google Scholar

3. Mumby S, Adcock S. Recent evidence from omic analysis for redox signalling and mitochondrial oxidative stress in COPD. J Inflamm. (2022) 19:10. doi: 10.1186/s12950-022-00308-9

PubMed Abstract | CrossRef Full Text | Google Scholar

4. Miravitlles M, Calle M, Molina J, Almagro P, Gómez JT, Trigueros JA, et al. Spanish COPD guidelines (GesEPOC) 2021: updated pharmacological treatment of stable COPD. Arch Bronconeumol. (2022) 58:69–81. doi: 10.1016/j.arbres.2021.03.026

PubMed Abstract | CrossRef Full Text | Google Scholar

5. Schols AMWJ, Broekhuizen R, Weling-Scheepers CA, Wouters EF. Body composition and mortality in chronic obstructive pulmonary disease. Am J Clin Nutr. (2005) 82:53–9. doi: 10.1093/ajcn.82.1.53

PubMed Abstract | CrossRef Full Text | Google Scholar

6. Celli BR, Cote CG, Marin JM, Casanova C, Montes de Oca M, Mendez RA, et al. The body-mass index, airflow obstruction, dyspnea, and exercise capacity index in chronic obstructive pulmonary disease. N Engl J Med. (2004) 350:1005–12. doi: 10.1056/NEJMoa021322

PubMed Abstract | CrossRef Full Text | Google Scholar

7. Waatevik M, Johannessen A, Gomez Real F, Aanerud M, Hardie JA, Bakke PS, et al. Oxygen desaturation in 6-min walk test is a risk factor for adverse outcomes in COPD. Eur Respir J. (2016) 48:82–91. doi: 10.1183/13993003.00975-2015

PubMed Abstract | CrossRef Full Text | Google Scholar

8. Fermont JM, Masconi KL, Jensen MT, Ferrari R, Di Lorenzo VAP, Marott JM, et al. Biomarkers and clinical outcomes in COPD: a systematic review and meta-analysis. Thorax. (2019) 74:439–46. doi: 10.1136/thoraxjnl-2018-211855

PubMed Abstract | CrossRef Full Text | Google Scholar

9. Kirkham PA, Barnes PJ. Oxidative stress in COPD. Chest. (2013) 144:266–73. doi: 10.1378/chest.12-2664

PubMed Abstract | CrossRef Full Text | Google Scholar

10. Lee C, Zeng J, Drew BG, Sallam T, Martin-Montalvo A, Wan J, et al. The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance. Cell Metab. (2015) 21:443–54. doi: 10.1016/j.cmet.2015.02.009

PubMed Abstract | CrossRef Full Text | Google Scholar

11. Reynolds JC, Lai RW, Woodhead JST, Joly JH, Mitchell CJ, Cameron-Smith D, et al. MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis. Nat Commun. (2021) 12:470. doi: 10.1038/s41467-020-20790-0

PubMed Abstract | CrossRef Full Text | Google Scholar

12. Kim KH, Son JM, Benayoun BA, Lee C. The mitochondrial-encoded peptide MOTS-c translocates to the nucleus to regulate nuclear gene expression in response to metabolic stress. Cell Metab. (2018) 28:516–24. doi: 10.1016/j.cmet.2018.06.008

PubMed Abstract | CrossRef Full Text | Google Scholar

13. Merry TL, Chan A, Woodhead JST, Reynolds JC, Kumagai H, Kim SJ, et al. Mitocondrial derived peptides in energy metabolism. Am J Physiol Endocrinol Metab. (2020) 319:E659–66 doi: 10.1152/ajpendo.00249.2020

PubMed Abstract | CrossRef Full Text | Google Scholar

14. Swarnabala S, Gattu M, Perry B, Cho Y, Lockey RF, Kolliputi N. Romo1 links oxidative stress to mitochondrial integrity. J Cell Commun Signal. (2015) 9:73–5. doi: 10.1007/s12079-014-0249-3

PubMed Abstract | CrossRef Full Text | Google Scholar

15. Ye L, Mao S, Fang S, Zhang J, Tan Y, Gu W. Increased serum Romo1 was correlated with lung function, inflammation, and oxidative stress in chronic obstructive pulmonary disease. Inflammation. (2019) 42:1555–60. doi: 10.1007/s10753-019-01017-x

PubMed Abstract | CrossRef Full Text | Google Scholar

16. Global Strategy for the Diagnosis Management and Prevention of Chronic Obstructive Pulmonary Disease (2022). Global Initiative for Chronic Obstructive Lung Disease. (2022). Available online at: https://goldcopd.org/https://goldcopd.org/2022-gold-reports-2 (accessed July 31, 2022).

Google Scholar

17. García-Río F, Calle M, Burgos F, Casan P, Del Campo F, Galdiz JB, Giner J, González-Mangado N, Ortega F, Puente Maestu L. Spanish society of pulmonology and thoracic surgery (SEPAR) spirometry. Arch Bronconeumol. (2013) 49:388–401. doi: 10.1016/j.arbr.2013.07.007

PubMed Abstract | CrossRef Full Text | Google Scholar

18. Barreiro E, Bustamante V, Cejudo P, Gáldiz JB, Gea J, de Lucas P, et al. SEPAR guidelines for the evaluation and treatment of muscle dysfunction in patients with chronic obstructive pulmonary disease. Arch Bronconeumol. (2015) 51:384–95. doi: 10.1016/j.arbr.2015.04.027

PubMed Abstract | CrossRef Full Text | Google Scholar

19. Cederholm T, Bosaeus I, Barazzoni R, Bauer J, Van Gossum A, Klek S, et al. Diagnostic criteria for malnutrition - an ESPEN consensus statement. Clin Nutr. (2015) 34:335–40. doi: 10.1016/j.clnu.2015.03.001

PubMed Abstract | CrossRef Full Text | Google Scholar

20. Casanova C, Cote C, Marin JM, Pinto-Plata V, de Torres JP, Aguirre-Jaíme A, et al. Distance and oxygen desaturation during the 6-min walk test as predictors of long-term mortality in patients with COPD. Chest. (2008) 134:746–52. doi: 10.1378/chest.08-0520

PubMed Abstract | CrossRef Full Text | Google Scholar

21. Husebø GR, Grønseth R, Lerner L, Gyuris J, Hardie JA, Bakke PS, et al. Growth differentiation factor-15 is a predictor of important disease outcomes in patients with COPD. Eur Respir J. (2017) 49:1601298. doi: 10.1183/13993003.01298-2016

PubMed Abstract | CrossRef Full Text | Google Scholar

22. Kempf T, von Haehling S, Peter T, Allhoff T, Cicoira M, Doehner W, et al. Prognostic utility of growth differentiation factor-15 in patients with chronic heart failure. J Am Coll Cardiol. (2007) 50:1054–60. doi: 10.1016/j.jacc.2007.04.091

PubMed Abstract | CrossRef Full Text | Google Scholar

23. Ramanjaneya M, Bettahi I, Jerobin J, Chandra P, Abi Khalil C, Skarulis M, et al. Mitochondrial-derived peptides are down regulated in diabetes subjects. Front Endocrinol. (2019) 10:331. doi: 10.3389/fendo.2019.00331

PubMed Abstract | CrossRef Full Text | Google Scholar

24. Du C, Zhang C, Wu W, Liang Y, Wang A, Wu S, et al. Circulating MOTS-c levels are decreased in obese male children and adolescents and associated with insulin resistance. Pediatr Diabetes. (2018) 19:1058–64. doi: 10.1111/pedi.12685

PubMed Abstract | CrossRef Full Text | Google Scholar

25. Baylan FA, Yarar E. Relationship between the mitochondria-derived peptide MOTS-c and insulin resistance in obstructive sleep apnea. Sleep Breath. (2021) 25:861–6 doi: 10.1007/s11325-020-02273-0

PubMed Abstract | CrossRef Full Text | Google Scholar

26. Qin Q, Delrio S, Wan J, Widmer RJ, Cohen P, Lerman LO, et al. Downregulation of circulating MOTS-c levels in patients with coronary endothelial dysfunction. Int J Cardiol. (2018) 254:23–7 doi: 10.1016/j.ijcard.2017.12.001

PubMed Abstract | CrossRef Full Text | Google Scholar

27. Liu C, Gidlund EK, Witasp A, Qureshi AR, Söderberg M, Thorell A, et al. Reduced skeletal muscle expression of mitochondrial-derived peptides human and MOTS-c and Nrf2 in chronic kidney disease. Am J Physiol Renal Physiol. (2019) 317:F1123–31 doi: 10.1152/ajprenal.00202.2019

PubMed Abstract | CrossRef Full Text | Google Scholar

28. Tekin S, Bir LS, Avci E, Senol H, Tekin I, Cinkir U. Comparison of serum mitochondrial open reading frame of the 12S rRNA-c (MOTS-c) levels in patients with multiple sclerosis and healthy controls. Cureus. (2022) 14:e26981. doi: 10.7759/cureus.26981

PubMed Abstract | CrossRef Full Text | Google Scholar

29. Salas-Lucia F, Bianco AC. T3 levels and thyroid hormone signaling. Front Endocrinol. (2022) 13:1044691 doi: 10.3389/fendo.2022.1044691

PubMed Abstract | CrossRef Full Text | Google Scholar

30. Hyatt JK. MOTS-c increases in skeletal muscle following long term physical activity and improves acute exercise performance after a single dose. Physiol Rep. (2022) 10:e15377 doi: 10.14814/phy2.15377

PubMed Abstract | CrossRef Full Text | Google Scholar

31. Dieli-Conwright CM, Sami N, Norris MK, Wan J, Kumagai H, Kim SJ, et al. Effect of aerobic and resistance exercise on the mitochondrial peptide MOTS-c in hispanic and non-hispanic white breast cancer survivors. Sci Rep. (2021) 11:16916. doi: 10.1038/s41598-021-96419-z

PubMed Abstract | CrossRef Full Text | Google Scholar

32. Garcia-Benlloch S, Revert-Ros F, Blesa JR, Alis R. MOTS-c promotes muscle differentiation in vitro. Peptides. (2022) 155:170840 doi: 10.1016/j.peptides.2022.170840

PubMed Abstract | CrossRef Full Text | Google Scholar

33. Neves CDC, Lage VKS, Lima LP, Matos MA, Vieira ELM, Teixeira AL, et al. Inflammatory and oxidative biomarkers as determinants of functional capacity in patients with COPD assessed by 6-min walk test-derived outcomes. Exp Gerontol. (2021) 152:111456. doi: 10.1016/j.exger.2021.111456

PubMed Abstract | CrossRef Full Text | Google Scholar

34. Ye L, Quian Y, Li Q, Fang S, Yang Z, Tan Y, et al. Serum Romo1 is significantly associated with disease severity in patients with obstructive sleep apnea syndrome. Sleep Breath. (2018) 22:743–8 doi: 10.1007/s11325-017-1606-2

PubMed Abstract | CrossRef Full Text | Google Scholar