Ruhui Zhang1
Ge Jin
Yasheng Zhan
Lisha Shen
Yake Yao
Qiqi Gao
Qing Yang
Jianying Zhou
Hua Zhou- 1Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
- 2Department of Pathology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
- 3State Key Lab for Diagnostic and Treatment of Infectious Diseases, First Affiliated Hospital, Zhejiang University, Hangzhou, China
Mucormycosis is a rare and invasive fungal infection with high mortality. Cases of invasive pulmonary mucormycosis that involve allergic reactions such as allergic bronchopulmonary mycosis are rarely reported. Herein, we describe a case of invasive pulmonary mucormycosis overlapping with allergic diseases in a patient who presented with eosinophilia and high total plasma immunoglobulin E (IgE). The patient was successfully treated with systemic corticosteroids (initial dose of prednisolone approximately 0.5 mg/kg per day, total duration less than 3 months) combined with posaconazole antifungal therapy. The treatment resulted in recovery of peripheral-blood eosinophil count and total plasma IgE, and significant reduction in lung lesions. A subsequent lobectomy was performed. The findings in this case indicate that systemic corticosteroid therapy may contribute to the treatment of pulmonary mucormycosis combined with allergic factors.
Introduction
A pulmonary fungal infection can simultaneously cause both invasive infection and allergic reactions such as allergic bronchopulmonary mycosis (ABPM), and this can be associated with undesirable therapeutic effects and poor outcomes (1). Pulmonary mucormycosis is an invasive fungal disease with high mortality (2). Herein, we describe a case of invasive pulmonary mucormycosis with concurrent allergic reaction in a patient who presented with eosinophilia and high plasma IgE, and in whom treatment with systemic corticosteroid (SCS) combined with posaconazole (antifungal therapy) achieved a favorable outcome.
Case Description
A previously well 38-year-old man with protracted cough, sputum, and chest pain was diagnosed with pulmonary mucormycosis via a lung puncture. He had no history of diabetes mellitus, HIV infection, atopy, or any other disease. Chest computed tomography revealed a mass in the right upper lobe of the lung involving the right clavicular region, axillary mediastinum, hilar lymph node, and chest wall. A lung biopsy was performed and indicated pulmonary mucormycosis. Posaconazole oral suspension (200 mg every 6 h) was started on day 19.
Radiological assessment on day 118 indicated no reduction in the size of the mass after 3 months of posaconazole therapy (Figure 1). Blood tests revealed a white blood cell count of 18,100/μl (neutrophils 6,730/mm3, lymphocytes 1,910/mm3, and eosinophils 1,550/mm3), a platelet count of 278,000/μl, and a hemoglobin value of 11.4 g/dl. C-reactive protein level was 28.9 mg/L (normal range 0–8 mg/L). Total IgE level was > 5,000 kU/L (normal range 0–100 kU/L). Bronchoscopy revealed stenosis of the apical segment of the upper lobe, with no mucus plug. Repeated lung puncture on day 118 indicated granulomatous lesions with infiltration of a large number of lymphocytes and eosinophils. Immunofluorescence staining suggested mucormycosis infection (Figure 2). Tissue culture and metagenomic next-generation sequencing both indicated Rhizopus infection. Amphotericin B (0.7 mg/kg per day) with concurrent intravenous dexamethasone (5 mg per day) was started on day 120 because of failure to respond to posaconazole, but amphotericin B was stopped on day 128 after a total dose of 170 mg because of renal dysfunction.
Figure 1. Case time line. Chest computed tomography on day 118 depicted a mass in the right upper lobe of the lung involving the right chest wall, and there was no reduction in its size after 3 months of treatment with oral posaconazole suspension. Concurrent amphotericin B and dexamethasone were started after repeat diagnostic lung biopsy on day 120. Amphotericin B was stopped on day 128. Systemic corticosteroid (SCS, prednisone tablets) was given after repeat imaging on day 131 and depicted a marked reduction in mass size. Radiology on day 183 indicated substantial improvement in the lung lesions with concurrent weaning of SCS. The patient underwent subsequent lobectomy on day 195, and posaconazole was used during the following 3 months. He was well, with no radiological evidence of relapse on day 342 follow-up. Reduction in peripheral eosinophil count and total serum IgE level was observed after initial SCS and anti-fungal therapy.
Figure 2. Pathological findings in lung tissue samples. (A) High-magnification image of a hematoxylin-eosin-stained specimen (bar = 100 μm). Infiltration of eosinophils was observed (arrow). (B) Gomori's methenamine silver staining revealed filamentous fungi with broad and nonseptate hyphae in the lung lesion (arrow; bar =100 μm). (C) Periodic acid Schiff staining revealed filamentous fungi with broad and nonseptate hyphae in the lung lesion (arrow; bar = 100 μm).
Repeat imaging on day 131 revealed a marked reduction in the size of the mass (Figure 1). Given the eosinophilia and elevated serum IgE, we hypothesized that an allergic reaction had favored disease progression. Accordingly, SCS was administered as follows: prednisone tablets 0.5 mg/kg for 1 week, followed by 0.25 mg/kg for 3 weeks, then tapered by 5 mg every week continued for a total duration of 2 months. IgE level and eosinophil count in peripheral blood decreased during treatment with SCS and posaconazole oral suspension. Follow-up radiology on day 183 indicated substantial improvement in the lung lesions (Figure 1). The patient underwent subsequent right upper lobe lobectomy on day 195, and post-surgery he was administered a further 3 months of posaconazole treatment. The patient was well with no clinical or radiological evidence of relapse on day 342 follow-up (Figure 1).
Discussion
Concurrent invasive pulmonary mucormycosis and host allergic reaction is relatively rare. A systematic PubMed search suggested that to date only a few case reports have described pulmonary mucormycosis with ABPM or increased eosinophil count and total IgE level (3–5). This patient exhibited increased total serum IgE (> 1,000 IU/ml) and peripheral blood eosinophilia (> 500/mm3). Together with substantial infiltration of eosinophils in mucoraceous lesions, these observations indicated an allergic response triggered by Mucor that was poorly responsive to conventional antifungal therapies. We postulate that this patient probably developed ABPM during the course of pulmonary mucormycosis based on the modified International Society for Human and Animal Mycology working group criteria (6, 7). Notably however, the definition and diagnostic criteria of ABPM are still controversial (8).
The management of ABPM includes two main components, SCS anti-inflammatory therapy and antifungal therapy, to alleviate fungal burden (9). Although there are no previous reports on successful SCS treatment for mucormycosis-induced ABPM (3–5), there is emerging evidence that SCS combined with immunotherapy is effective in patients with invasive fungal infections associated with T-helper 2 immune responses. Anti-interleukin 5 (mepolizumab) therapy can effectively dampen aberrant T-helper 2 responses, and may be a new therapeutic option for invasive pulmonary mycosis (10). In this patient, low-dose and medium-dose SCS and concurrent posaconazole treatment rapidly achieved symptomatic control, reduced the size of the lung mass, and suppressed peripheral blood eosinophilia. We assume that the major response to SCS was mediated by suppression of eosinophilic inflammation in invasive pulmonary mucormycosis. In a randomized controlled trial of ABPM, it was reported that a median dose (0.5 mg/kg per day) of prednisolone treatment may be as effective as or safer than a higher dose (0.75 mg/kg per day); hence a lower dose and a shorter duration may be better options (11).
This report has several limitations, which include the absence of amphotericin B liposome therapy and posaconazole therapeutic drug monitoring due to drug availability. The patient only received treatment with posaconazole suspension, because posaconazole tablets and intravenous formulation were not marketed, and because liposomal amphotericin B was not available in China at the time. Global mucormycosis guidelines recommend liposomal amphotericin B as first-line treatment. In clinical studies, plasma concentrations in patients administered with sustained-release posaconazole tablets and intravenous formulations were higher than those in patients administered with oral suspensions (12, 13). We speculate that this patient had ABPM caused by Mucor based on clinical evidence. Direct evidence of allergy against Mucorales such as mucor-specific IgE was not obtained because of experimental limitations. We concluded that there was clear evidence of type 2 hypersensitivity (raised IgE and eosinophil count) that responded to prednisolone.
To our knowledge, this is the first report to describe the administration of SCS as part of successful therapy for invasive pulmonary mucormycosis. It also provides important clinical insight indicating that invasive pulmonary mucormycosis can be present with severe allergic reaction.
Data Availability Statement
The original contributions presented in the study are included in the article/supplementary material, further inquiries can be directed to the corresponding author.
Ethics Statement
This study was conducted in accordance with the Declaration of Helsinki, and reviewed and approved by the Research Ethics Committee of the First Affiliated Hospital of Zhejiang University (IIT20210130A). The patient provided written informed consent for the publication of clinical information including lung CT and pathological images.
Author Contributions
All authors listed have made a substantial, direct, and intellectual contribution to the work and approved it for publication.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher's Note
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Abbreviations
IgE, immunoglobin E; SCSs, systemic corticosteroids; ABPM, allergic bronchopulmonary mycosis; CT, computedtomography; PET-CT, positron emission-CT; WBC, white blood cell; CRP, C-reactive protein; AmB, amphotericin B; ISHAM, International Society for Human and Animal Mycology.
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Keywords: allergic bronchopulmonary mycosis, mucor, invasive pulmonary mucormycosis, glucocorticoid, IgE
Citation: Zhang R, Jin G, Zhan Y, Shen L, Yao Y, Gao Q, Yang Q, Zhou J and Zhou H (2022) Allergic Bronchopulmonary Mycosis Caused by Mucor Overlapping With Invasive Pulmonary Mucormycosis: A Case Report. Front. Med. 9:831213. doi: 10.3389/fmed.2022.831213
Received: 08 December 2021; Accepted: 12 January 2022;
Published: 24 February 2022.
Edited by:
Amelia Licari, University of Pavia, ItalyReviewed by:
Ritesh Agarwal, Post Graduate Institute of Medical Education and Research (PGIMER), IndiaGiuseppe Fabio Parisi, University of Catania, Italy
Copyright © 2022 Zhang, Jin, Zhan, Shen, Yao, Gao, Yang, Zhou and Zhou. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Hua Zhou, zhouhua1@zju.edu.cn