This article is a correction to:
Role of HK2 in the Enzootic Cycle of Borrelia burgdorferi
Qiang Liu
Haijun Xu
Yan Zhang
Jing Yang
Jimei Du
Yan Zhou1
X. Frank Yang
Yongliang Lou- 1Wenzhou Key Laboratory of Sanitary Microbiology, Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
- 2Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN, United States
- 3State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou, China
- 4Optometry and Eye Hospital and School of Ophthalmology, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, China
A Corrigendum on
Role of HK2 in the Enzootic Cycle of Borrelia burgdorferi
by Liu, Q., Xu, H., Zhang, Y., Yang, J., Du, J., Zhou, Y., et al. (2020). Front. Med. 7:573648. doi: 10.3389/fmed.2020.573648
In the original article, there was a mistake in Figure 5 as published. Due to an error in compiling multi-panel images, a gap between the image of “B31-A3” and the image of “B31A3/flaBp-HD-GYP; B31A3/flaBp-hk2” was omitted. In this figure, unphosphorylated Rrp2 (lower lane) serves as an internal control for each sample. Overproduction of an unrelated protein HD-GYP (B31A3/flaBp-HD-GYP) serves as the negative control for overproduction of Hk2 (B31A3/flaBp-hk2), showing a reduction of Rrp2 phosphorylation by overexpression of Hk2. The corrected Figure 5 appears below.
FIGURE 5
Figure 5. Overexpressing HK2 reduces the level of phosphorylated Rrp2 in B. burgdorferi. Phos-tag SDS-PAGE and immunoblotting was used to detect both phosphorylated and dephosphorylated Rrp2 in the cell. Wild-type B. burgdorferi B31A3, B31A3 carrying a shuttle vector harboring a unrelated protein HD-GYP (B31A3/flaBp-HD-GYP), or B31A3 carrying a shuttle vector harboring a hk2 gene driven by a flaB promoter GYP (B31A3/flaBp-hk2), were harvested at mid-log phase and cell lysates were prepared and separated on 7.5% SDS-PAGE containing 0, 5, 10, and 25 uM Phos-tag followed by immunoblotting using anti-Rrp2 antibody. p-Rrp2, the band corresponds to phosphorylated Rrp2. As a unphosphorylated Rrp2 control, B31A3 was also treated by boiling (lane 2) prior to Phos-tag SDS-PAGE (Rrp2 phosphorylation is unstable and sensitive to heat).
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
Keywords: lyme disease, Borrelia (Borreliella) burgdorferi, two-component system (TCS), Rrp2-HK2, OspC
Citation: Liu Q, Xu H, Zhang Y, Yang J, Du J, Zhou Y, Yang XF and Lou Y (2021) Corrigendum: Role of HK2 in the Enzootic Cycle of Borrelia burgdorferi. Front. Med. 8:668709. doi: 10.3389/fmed.2021.668709
Received: 17 February 2021; Accepted: 01 March 2021;
Published: 31 March 2021.
Edited and reviewed by: Ying Zhang, Zhejiang University, China
Copyright © 2021 Liu, Xu, Zhang, Yang, Du, Zhou, Yang and Lou. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: X. Frank Yang, xfyang@iu.edu; Yongliang Lou, lyl@wmu.edu.cn