Skip to main content

ORIGINAL RESEARCH article

Front. Mar. Sci.
Sec. Marine Biotechnology and Bioproducts
Volume 11 - 2024 | doi: 10.3389/fmars.2024.1461607
This article is part of the Research Topic Harnessing Marine Natural Products for Innovative Drug Discovery and Global Health Solutions View all articles

Harnessing Marine Bacteria for Next-Gen Antibiotics: Potent inhibition of S.aureus & Riemerella anatipestifer Through In-Vitro, Omics and Chemoinformatics Approach with Enhanced Production of Secondary Metabolites through Zinc Sulphate

Provisionally accepted
  • Shanghai Jiao Tong University, Shanghai, China

The final, formatted version of the article will be published soon.

    The rise of bacterial infections and increasing antibiotic resistance underscores an urgent need for new, effective antimicrobial agents with marine bacteria offering a unique and promising source for novel antibiotic compounds to combat persistent and emerging pathogens. In this research, five compounds were achieved from marine Streptomyces sp, C2-13 and their yield was enhanced by the addition of zinc sulphate 0.5mM. All compounds have been evaluated for its antibacterial activity against multiple pathogens among which good activity was achieved against S.aureus, while potent activity was achieved against Riemerella anatipestifer with its IC50 value on 200 µm and bactericidal effect on 300 µm. Among all compounds, 4 was more active against both pathogens. Transcriptome analysis of active compound 4 showed its antibacterial effect on R. anatipestifer by inhibiting 30 and 50S ribosomal subunits, resistance mechanisms and gliding motility proteins IX secretion system (T9SS), interfering with protein translations process, secretion system, defense and resistance mechanisms, ultimately resulting in effective inhibition of normal bacterial growth and its motility. To investigate the anti-bacterial mechanism, all compounds were docked with two enzymes, and TLR-4 protein for predicting vaccine construct, and the best docking score was achieved against RMFP with the highest score of -12.9 for compound 4. In-silico cloning was carried out to ensure the expression of proteins generated and were cloned using E.coli as a host. The simulation studies have shown that both compound 4-RMFP and TLR-4-RMFP complex are stable with the system. To best known to our knowledge this is the first study investigating marine bacterial metabolites against R. anatipestifer with their anti-bacterial mechanism and enhancing its yield through addition of zinc sulphate ions.

    Keywords: anti-bacterial, Streptomyces, Zinc sulphate, Transcriptome, Staphylococcus aureus, Riemerella anatipestifer

    Received: 08 Jul 2024; Accepted: 29 Nov 2024.

    Copyright: © 2024 Hassan and Jin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Syed Shams ul Hassan, Shanghai Jiao Tong University, Shanghai, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.