Xinghai Liu
Xinyue Tang†
Muyan Chen
Gang Ni
Yujia Yang- The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, China
Sea cucumber has emerged as a crucial economic species in aquaculture in China because of its remarkable nutritional and medicinal value. However, wild sea cucumber populations have experienced a decline due to overfishing and environmental factors, underscoring the urgent need for genetic resource conservation and biotechnology innovation within the sea cucumber aquaculture and breeding industry. The development of the sea cucumber industry is still impeded by challenges and difficulties. Nevertheless, significant progress has been made through the utilization of molecular markers, which have effectively addressed a number of fisheries and aquaculture issues. In recent years, diverse types of molecular markers including mitochondrial DNA, microsatellites, and SNP markers have been developed and extensively applied in various aspects of sea cucumber research. These markers play vital roles in genetic sex identification, germplasm resource evaluation, population structure assessment, as well as marker-assisted breeding in marine ranching and sea cucumber aquaculture and breeding industry. This review provides an overview of the fundamental principles, functions, and characteristics associated with various markers employed across various sea cucumber species while also discussing their applications within different aspects of the sea cucumber fisheries, aquaculture, and breeding industry.
1 Introduction
Sea cucumber is a marine invertebrate belonging to the class Holothuroidea with the phylum Echinodermata. In general, sea cucumbers appear in a cylindrical, highly contractile body with numerous papillae on the dorsal and tube feet on the ventral side. Sea cucumbers live in a wide variety of different habitats, including seaweed ecosystems and coral reefs, ranging from shallow water to depths of more than 3,000 meters (Sulardiono et al., 2022). They feed on microalgae, organic debris, and material in sediments (Madduppa et al., 2017). There are more than 1,700 species of sea cucumber in the world, of which about 50–60 are edible (Elvevoll et al., 2022). Total sea cucumber fisheries increased from 26,000 tonnes of live weight in 2007–2016 to 48,000 tonnes in 2018–2019, with a slight decline to 43,000 tonnes in 2020 (FAO). Most cultured sea cucumber species belong to the order Aspidochirotida, including two sister groups, Holothuriidae and Stichopodidae (Wen et al., 2010). In Asian countries, sea cucumber is a commercially important aquaculture species due to its high nutritional and medicinal value. The wild sea cucumber populations have declined because of overfishing and environmental factors (Peng et al., 2012).
To increase the yield of sea cucumber, the sea cucumber breeding industry is rising gradually. Since the 19th century, sea cucumber farming in most countries has experienced boom-and-bust cycles, with the exception of China, where the majority of sea cucumbers have come from the aquaculture industry since the late 1980s (Liu, 2016). Even though sea cucumber aquaculture has been developed in the past decades, a series of problems occurred that could affect the larva development and juvenile growth, mainly related to disease control, germplasm conservation, and large-scale production in sea cucumber industry (Du et al., 2012). Among these, the slow growth rate is one of the most important issues in the sea cucumber industry (Cui et al., 2021). To meet the increasing demand for sea cucumber in the market, sea cucumber aquaculture and management using molecular methods or based genomic methods are quite important.
The sea cucumber breeding industry requires the application of traditional and novel molecular methods to benefit production and ensure sustainable development. Genetic breeding has been widely used in the breeding of aquaculture species, which focuses on genetic variations among populations and individuals to improve different types of performance traits. Molecular markers are commonly used molecular methods for detecting biological diversity at the DNA level, which is a powerful informatic molecular tool in genetic breeding and population genetics. Commonly used molecular markers include mitochondrial DNA (mtDNA), restricted fragments length polymorphism (RFLP), random amplification polymorphic DNA (RAPD), microsatellite (SSR), and single nucleic polymorphism (SNP), which have been widely used for species identification, population genetics, genetic breeding, marker-assisted selection, stock enhancement, and aquaculture management (Maqsood, 2017).
At the end of the 20th century, with the emergence of restriction enzymes and polymerase chain reaction (PCR), many molecular marker technologies have been developed and widely used. RFLP marker is a highly polymorphic and co-dominant marker that can be used for species identification, which is rarely used at present due to its complex process (Wen et al., 2010). RAPD marker is a simple technique without molecular hybridization and radioautography, which can be used to evaluate the geographical origin of species and genetic diversity analysis (Yun et al., 2017). Microsatellite marker is abundant and widely distributed throughout the genome (Oliveira et al., 2006), which is a simple and fast marker that have high mutation rates and reliable results, which can identify sea cucumber species, which will benefit germplasm conservation and management (Zhan et al., 2007). Subsequently, the advent of single nucleic polymorphism (SNP) molecular marker technology made it possible to perform genetic analysis of the genome at a precise level (Oliveira and Azevedo, 2022). Microsatellite marker and SNP marker are ideal molecular markers because of their high polymorphism and co-dominant inheritance (Maqsood, 2017), which have been widely used in population structure analysis (Kanno et al., 2006), genetic linkage map construction (Yan et al., 2013), QTL mapping (Chen and Li, 2007), marker-assisted selection (MAS) (Wang et al., 2009). Mitochondrial marker is a simple, fast, reliable molecular marker that is inherited from maternal lineage, which have high mutation rates and can be used in species identification, population genetic structure analysis, and phylogenetic analysis (Sulardiono et al., 2022). The advantages and disadvantages of various types of molecular markers are summarized in Table 1. At present, molecular markers are quite useful in the sea cucumber aquaculture and breeding industry. For instance, using sex-specific tags and SNPs to identify the sex can develop and breed a single-sex population, which could improve breeding efficiency, and lay a molecular foundation for studying the sex determination mechanism of sea cucumber (Cui et al., 2021); The high-density genetic linkage map of sea cucumber was constructed based on SNPs, which laid a foundation for mapping and analyzing QTL for performance traits like growth rate (Wei et al., 2021). This review introduces and summarizes the development and application of different types of molecular markers and their based genomic methods in sea cucumbers (Figure 1, Table 2) and examines the present status and prospects in fisheries, aquaculture, and industry.
Table 1 Characteristics, advantages, and disadvantages of various types of molecular markers.
Figure 1 Time history of molecular markers and their based genomic methods applied in sea cucumbers.
Table 2 Development and application of various types of molecular markers in sea cucumbers.
2 Development of various molecular markers in sea cucumber
2.1 Mitochondrial DNA markers
The DNA barcoding using mitochondrial COI (cytochrome C oxidase subunit I) and ITS (internal transcribed spacers) can be used for identifying species for Stichopus genus and analyzing genetic diversity and phylogenetic relationships (Madduppa et al., 2017; Sulardiono et al., 2022). More specifically, the DNA barcode mitochondrial COI has been used for identifying sea cucumber species in two main families, including Stichopodidae (Stichopus herrmanni, Stichopus ocellatus, Stichopus horrens, Stichopus monotuberculatus) and Holothuridae (Bohadschia bivittata, Actinopyga lecanora, and Holothuria leucospilota), which will benefit germplasm conservation and management (Madduppa et al., 2017).
Using the mitochondrial DNA marker, three local sea cucumber species in Indonesia, Crengek Gamete, Pace gamete, and Kuning gamete, were close relatives to Stichopus monotuberculatus (Sulardiono et al., 2022). A next-generation sequencing-based DNA mini-barcoding using COI has been developed to screen various commercial sea cucumber products and correct mislabeled for clearer labeling guidelines (Xing et al., 2021). The complete mitochondrial genome and cox1 DNA barcoding will be helpful for the sea cucumber breeding industry and studies on population genetic diversity and molecular ecology resources (Fan et al., 2012). In addition, a species-specific PCR of 16S rRNA has been used to authenticate 11 sea cucumber species from processed products, providing a rapid and useful protocol for correct labeling of commercial sea cucumber products (Wen et al., 2012a). By calculating the genetic distance of COI gene among different sea cucumber, Lu et al. proved the feasibility of COI as a DNA barcode for species identification, and the probes designed based on COI gene were used to identify four species of sea cucumber (Lv et al., 2011). Hu et al. used COI gene and 16S rRNA gene as target genes to identify sea cucumber species based on DNA barcoding technology, and the results showed that COI gene or 16S RNA gene could identify most species of sea cucumber (Hu et al., 2019). According to the specific primers for cox1 gene of Holothuria scabra, eDNA technology was used to investigate Holothuria resources in Weizhou Island, and the results showed that the eDNA monitoring method was more suitable and reliable than traditional methods (E et al., 2023).
2.2 Traditional molecular markers
A PCR-RFLP method was developed to identify six species of sea cucumbers in commercial products, which can authenticate species and detect fraudulent labeling (Wen et al., 2010). Another application of the PCR-RFLP method was to identify 16 commercial sea cucumber species from food products to correct the mislabeling (Zeng et al., 2018). Moreover, a PCR-RFLP method using restriction nuclease Sau3AI on 16S rRNA fragments has been developed to rapidly and inexpensively identify holothurian species with no need for taxonomical or genetic expertise (Madeira et al., 2018). DNA polymorphisms identified by RAPD-PCR technology were used to evaluate the geographical origin of A. japonicus and genetic diversity analysis (Yun et al., 2017). EST databases of sea cucumber species have been built, including Holothuria glaberrima during intestinal regeneration (Rojas-Cartagena et al., 2007), multiple tissues (body wall, intestine, respiratory tree) in Apostichopus japonicus (Yang et al., 2009), which were submitted to NCBI Genbank. EST databases have provided a rich resource for the development of molecular markers of sea cucumber species, especially microsatellite markers. SSR can be identified from EST data using screening programs, for example, SSRHUNTER (Chen and Li, 2007; Zhan et al., 2007; Peng et al., 2009; Jiang et al., 2011). In addition, SNP markers were characterized by mining EST data of A. japonicus and using a homogeneous Tm- (melting temperature) genotyping method (Yang et al., 2012; Dong et al., 2016).
2.3 Microsatellite markers
The majority of microsatellite markers in sea cucumber species were from an aquaculture species in the coastal area of the Northwest Pacific, Stichopus (Apostichopus) japonicus. The earliest 20 microsatellite markers of sea cucumber Apostichopus japonicus were developed by Kanno et al. in 2004, which could be excellent molecular markers to facilitate genetic structure analysis (Kanno et al., 2005). Eleven microsatellite markers were used to identify A. japonicus population from green and black color variants for further genetic population structure analysis and ecological research (Kanno et al., 2006). In sea cucumber Apostichopus japonicus, Hu et al. developed an efficient procedure for isolating a reasonable number of microsatellites for genetic analysis (Hu et al., 2007). Forty-five informative microsatellite markers (43 genomic SSRs and 2 EST-SSRs) were developed based on screening SSR-enriched library and EST library, which may contribute to population structure analysis, linkage map construction, stock enhancement, and species identification (Zhan et al., 2007). Eleven microsatellite markers derived from expressed sequence tags were developed to investigate population structure and conduct parentage analysis and QTL mapping (Chen and Li, 2007). Microsatellite markers associated with body weight were developed for not only molecular breeding but also marker-assisted selection (MAS) (Wang et al., 2009). Jiang et al. have developed 43 EST-SSR markers for future studies in population genetics and genomics (Jiang et al., 2011). Twenty novel microsatellite markers from Apostichopus japonicus were used for cross-species application in Parastichopus parvimensis from the United States and Pathallus mollis from Peru, which could facilitate genetic variation assessment and genetic resource management among Holothuroidea populations (Liao et al., 2011). A larger number of ~70 microsatellite markers were developed for the implementation of marker-assisted selection and population genetic studies (Peng et al., 2012). Furthermore, 45 gene-derived microsatellite markers were identified from transcriptome sequencing, which could be useful for comparative genomics studies, genetic linkage mapping, and molecular breeding like MAS (Chen et al., 2013). A total of 8 highly polymorphic microsatellite markers were used for genetic structure studies between wild and hatchery populations and implications in breeding programs and stocking strategies (An et al., 2013). Ten microsatellite markers have revealed the genetic variation and population structure of A. japonicus in Peter the Great Gulf, Japan. In studying microsatellite markers, the detected high heterogeneity among populations might be due to several factors: stochastic larval settlement at a location, and selection pressures on QTL (Yagodina et al., 2022).
Ten microsatellite markers were developed in a tropical sea cucumber species Stichopus chloronotus to investigate the genetic structure and gene flow among populations (Taquet et al., 2011). Eighteen microsatellite markers from another sea cucumber species Holothuria scabra were characterized, which were beneficial for genetic structure and gene flow studies for sea cucumber aquaculture and management (Fitch et al., 2013). A total of another 9 microsatellite markers of H. scabra were developed for conservation strategy and investigation of genetic diversity and population structure (Li et al., 2015a). Eight microsatellite markers were identified in a tropical species Holothuria leucospilota, which could be beneficial for genetic structure, population diversity, and germplasm conservation (Dai et al., 2015). Shangguan et al. identified 16 additional microsatellite markers from H. leucospilota that could be used for studies in genetic diversity and population structure and application in cultivation and conservation strategies (Shangguan et al., 2015a). Li et al. identified 9 microsatellite markers in another tropical sea cucumber Stichopus horrens (Selenka) that could be used as markers to study its conservation and breeding, population structure, phylogeny and evolutionary studies of Holothuroidea (Li et al., 2015b). 9 more microsatellite markers have been characterized in S. horrens, which could be helpful for conservation strategy and management, population structure and genetic diversity of S. horrens (Shangguan et al., 2015b). 9 novel microsatellite markers have been identified from Holothuria mammata, an exploited sea cucumber species in Mediterranean and North-Eastern Atlantic, which could be used for aquaculture and conservation management, gene flow, and genetic diversity assessment (Henriques et al., 2016). Furthermore, eight microsatellite markers in the West Atlantic sea cucumber species Holothuria grisea have been developed and used as markers for proper conservation and advancement in sustainable aquaculture of this species (Alves Pereira et al., 2018). Despite the popularity of SSR in diverse aspects of sea cucumber research, it is gradually replaced by genome-wide molecular markers such as SNPs.
2.4 Single nucleic polymorphism (SNP)
In A. japonicus, all identified 13 SNPs have two alleles and their heterozygosity could be used as molecular markers in analyzing population structure, population genetics, and QTL mapping (Sun et al., 2010). Fifteen more SNP markers were identified from ESTs and provided a complement to available molecular markers of A. japonicus (Yang et al., 2012). These SNPs may be implied in studies on genetic diversity, QTL mapping, and ecology analyses of A. japonicus (Yang et al., 2012). 26 SNP markers associated with defense mechanisms were developed by mutation scanning from unigene sequences in A. japonicus transcriptome, which may provide insights into genetic maps and comparative genomic studies (Gao et al., 2013). 51 gene-derived SNPs from ESTs using high-resolution melting can be used for MAS, genetic improvement, and aquaculture management (Dong et al., 2016). High throughput transcriptome analysis provided benefit to large-scale marker discovery of A. japonicus. 142,511 SNP markers and 6,417 microsatellite markers with high quality have been identified through transcriptome analysis, which can be used for gene discovery and functional genomic research (Zhou et al., 2014). A total of 219,860 SNP markers have been identified from tube foot transcriptome, providing plentiful resources in genetic and molecular ecology studies (Zhou et al., 2016).
3 Construction of genome assembly, genetic linkage map, and SNP array
Since 2017, the genome assemblies of sea cucumber species have been constructed using next-generation sequencing and third-generation sequencing, including one northern sea cucumber species A. japonicus, and three tropical sea cucumber species, H. scabra, H. leucospilota, H. glaberrima and S. monotuberculatus (Table 3). As the most common commercial sea cucumber species, the A. japonicus genome assembly have been constructed in 2017 (Zhang et al., 2017) and 2018 (Li et al., 2018) and updated in 2023 (Sun et al., 2023). The construction of genome assembly has been used to understand the molecular mechanisms on morphological evolution, visceral regeneration, saponin biosynthesis, aestivation regulation, providing a referable resource for understanding evolution and biodiversity of sea cucumber. In 2022, De novo genome assembly of H. scabra has been built using nanopore sequencing, which provides excellent genetic resource for studying genetic, phylogenetic, molecular biology, and breeding studies (Luo et al., 2022). In 2023, a draft genome of S. monotuberculatus was assembled using Nanopore sequencing, which provides insight into critical genes in fucosylated chondroitin sulfates biosynthetic pathway (Zhong et al., 2023). The high-resolution genome assembly of H. leucospilota was also built in 2023 using Illumina sequencing, Pacbio sequencing, BGI sequencing platform, which provides insights into molecular underpinnings of sacrificial organ expulsion in holothurian species (Chen et al., 2023).
Table 3 Construction of genome assembly and molecular markers-based genomic methods in sea cucumbers.
Genetic linkage maps are constructed based on the gene linkage and genetic recombination rates to infer the relative positions of genes or genetic markers on chromosomes (Cui et al., 2021). Up to date, a total of four genetic linkage maps of Apostichopus japonicus based on molecular markers have been developed for QTL mapping and marker-assisted selection breeding since 2009 (Table 3). The first genetic linkage map was built on 484 polymorphic AFLP and microsatellite markers, which provides the application of molecular breeding and understanding the genetic basis of economic traits in sea cucumber industry (Li et al., 2009). In 2013, a medium-density genetic linkage map using co-dominant microsatellite and SNP markers was built to benefit QTL mapping, marker-assisted selection, and comparative genomic studies (Yan et al., 2013). A high-density and high-resolution genetic map of 7,839 markers based on 2b-restriction site-associated DNA (2b-RAD) was constructed in 2015, which facilitates fine-scale QTL mapping, precise marker-assisted selection, and chromosome assignment for whole genome assembly (Tian et al., 2015). A 6,144 SNP-based high-density genetic map using genotyping-by-sequencing was built, which provides an important genomic resource for marker-assisted selection with high performance traits like growth traits (Sun et al., 2021). With the development of whole genome sequencing and new molecular techniques, genome-wide molecular markers like SNPs in an SNP array chip have been used as an efficient genotyping tool for genetic research and genome breeding programs in aquaculture. In 2022, a high-throughput 24 K SNP array genotyping platform, HaishenSNP24K, was developed for genetic studies and application in selection and breeding programs with minmax concave penalty regularized deep neural networks (DNN-MCP) (Lv et al., 2022). The new SNP array has significantly improved the genomic assessment of sea cucumber populations for quantitative performance traits, including wet or dry weight, and survival time (Lv et al., 2022).
4 Application of molecular markers and based genomic methods in fisheries, aquaculture, and industry
The molecular marker technology facilitates germplasm resource evaluation, assessment of genetic diversity and population structure, sex identification for live animal, and selection of desirable traits, which has been extensively applied in various aspects of the sea cucumber aquaculture industry (Figure 2). These applications play a pivotal role in enhancing the efficiency of industrialized aquaculture and breeding programs for sea cucumbers, enabling precise breeding strategies and conservation of germplasm resources.
Figure 2 Flow chart of molecular markers or based genomic methods in sea cucumber fisheries, aquaculture, and industry. (A) Germplasm resource evaluation and species identification; (B) Assessment of genetic diversity and population structure; (C) Genetic sex identification in aquaculture; (D) Marker-assisted selection and breeding.
4.1 Germplasm resource evaluation and species identification
Germplasm resource evaluation mainly includes population resource information, ecological factors, habitat conditions, environmental variables, external disturbance factors, and strain identification. Germplasm resource identification has significant implications not only in the discovery and development of new germplasm but also in hybrid and cryptic species identification and the utilization of hybrid advantages (Ding and Chang, 2020). Specific sequence analysis plays an important role in natural hybridization and gene flow between populations. For example, skeletal characteristics could not distinguish H. scabra, colour morphs of H. S. var.versicolor, and intermediate phenotype hybrids in the species boundary study, as their color patterns, morphology, and small-scale distribution patterns were different (Uthicke et al., 2005). Both of population genetics and 16S mtDNA sequence analysis were used to identify new species H. S. var.versicolor and individuals with intermediate phenotypes represented F1 hybrids, demonstrating that the opportunity for introgression exists (Uthicke et al., 2005). The conventional method based on their morphology or external characteristics for species identification have gradually developed towards molecular markers with high accuracy and polymorphism, such as RFLP, RAPD, SSR, and SNP (Lv, 2012). In general, germplasm resource evaluation by molecular markers includes four important steps: sample collection, DNA extraction, PCR amplification using molecular markers, gel electrophoresis or DNA sequencing. For instance, PCR-RFLP was used to develop a method of DNA barcoding-based sea cucumber species identification, and mitochondrial COI gene was used as a DNA barcode to achieve germplasm identification of five sea cucumber species (Lv, 2012). Wen et al. successfully identified 19 commercial sea cucumber species using PCR-RFLP method (Wen et al., 2012b). With the completion of mitochondrial genome sequencing of Apostichopus japonicus and other sea cucumber species at the National Center for Biotechnology Information (NCBI), the basic characteristics of the mitochondrial genome of the taxonomic class Holothuroidea have been revealed, which could benefit germplasm resource evaluation using molecular methods (Shen et al., 2011). The use of all these molecular markers in fisheries resource evaluation include species identification, geographical origin, and variant analysis. In sea cucumber industry, molecular methods could identify product ingredients and detect fraudulent labeling for food safety and better quality.
4.2 Assessment of genetic diversity and population structure
Molecular marker technology has been widely applied in the assessment of population genetic structure and genetic diversity, providing guidance for the sea cucumber aquaculture industry and germplasm resource protection. Bottom-culture farming in marine ranching is an important means of germplasm resource protection, and the evaluation of genetic diversity and population structure of its natural geographical populations is crucial. In general, this includes four important steps: sample collection, DNA extraction, genotyping or sequencing, and data analysis. Numerous sea cucumber studies have utilized mitochondrial DNA markers (e.g. COI, 16S) and nuclear DNA markers (e.g. SNP, SSR) for this purpose. Mitochondrial DNA markers are used to conduct haplotype diversity (h) and nucleotide diversity (π) for genetic diversity analysis, as well as FST (fixation index) and AMOVA (Analysis of molecular variance) for population analysis. For instance, Yan (2006) used COI molecular marker methods to investigate the genetic diversity and population genetic structure of A. japonicus from five geographic subgroups in the Dalian Sea area and revealed that these five geographical subgroups had high genetic diversity. A mitochondrial COI gene analysis for the population genetic structure of Holothuria atra in the waters surrounding the Hawaiian Islands in order to aid coral reef management (Skillings et al., 2011). Additionally, nuclear DNA markers have proven to be an effective method for evaluating genetic diversity, including allelic frequency, allelic richness, and heterozygosity, as well as population analysis including F-statistics analysis (Fixation indices), STRUCTURE analysis, DAPC (discriminant analysis of principal components), AMOVA. For instance, Wang et al. conducted genetic diversity and population structure assessments of 18 geographical populations of A. japonicus from sea ranching in Shandong, Hebei, and Liaoning provinces using specific SNP loci, thereby enabling continuous genetic monitoring of the northern natural habitats of sea cucumbers in China (Wang et al., 2023). Whole-genome SNP data was used to identify three genetically distinct subgroups within populations of Holothuria (Metriatyla) scabra from Fiji, revealing a model of genetic structure isolated by distance (Brown et al., 2022). An investigation of population genetics between Australia and Vietnam using SNP markers in H. Leucospilota was conducted to compare gene flow and genetic similarities among populations and analyzing population genetic structure (Chieu et al., 2023). Now land et al. used population genetic analysis based on microsatellite sequences to assess the population genetic structure of H. scabra in Papua New Guinea and a broader region of northern Australia, in order to determine the genetic diversity within subpopulations and investigate the genetic impact of environmental changes on wild populations (Nowland et al., 2017). Eight polymorphic microsatellite loci were successfully developed in H. leucospilota, which provided crucial molecular markers for studying population genetic diversity and conservation strategies in sea cucumbers (Dai et al., 2015). Liao et al. utilized SSR fingerprinting technology to analyze the genetic diversity and construct fingerprinting profiles of sea cucumber populations from different regions in China, South Korea, and Russia (Liao et al., 2021). The constructed fingerprinting profiles successfully differentiated eight populations and provided technical support for the protection of sea cucumber germplasm resources and the identification of different geographical populations (Liao et al., 2021). Furthermore, genetic diversity and structure assessments of wild populations and hatchery-produced H. scabra in New Caledonia were achieved using nine polymorphic microsatellite markers, aiming to develop an appropriate breeding plan for restocking (Riquet et al., 2022).
4.3 Identification of genetic sex
Due to the lack of visible sexual dimorphism in the external appearance of most sea cucumber species, the development and application of a method based on molecular markers is useful to identify the sex of several sea cucumber species (Table 4). Generally, the development of a sex-specific marker is realized through the assembly of female and male genome sequences, resequencing of female and male individuals, mapping and read alignment, identification of sex-specific region, and design and verification of sex-specific marker. The verification of an accurate and stable sex-specific marker is usually completed by wild populations in multiple geographical regions. Tested samples can be collected by anal swab or tissue dissection, and then genomic DNA can be extracted by multiple protocols. The genetic sex of sea cucumber individual is identified by PCR amplification using sex-specific marker. The utilization of sex-specific molecular markers facilitates the determination of individual gender and enhances the efficiency of artificial breeding in large scale aquaculture. In A. japonicus, a sex-specific marker was developed utilizing 2b-RAD technology, which holds significant implications for genetic breeding (Wei et al., 2021). In addition, a loop-mediated isothermal amplification (LAMP) method was established based on male-specific sequences, which could rapidly identify the genetic sex of A. japonicus (Cong et al., 2024). Furthermore, a rapid and non-destructive method was devised for sex identification in aquaculture practices involving another tropical sea cucumber species (Stichopus monotuberculatus) (Wu et al., 2022). A non-invasive and rapid method based on anal swab sampling using a LAMP system (PCR and loop-mediated isothermal amplification) was employed for sex identification in tropical sea cucumber Holothuria scabra (E et al., 2023).
Table 4 Development and application of sex-specific markers for genetic sex identification in sea cucumbers.
4.4 Marker-assisted selection and breeding
With the continuous development of breeding technologies, there is a gradual shift from traditional selective breeding and hybrid breeding techniques towards the integration of molecular marker-assisted breeding, whole-genome selection breeding, molecular design breeding, sex control, gene transfer, and gene editing (Ding et al., 2021). Molecular marker-assisted breeding is a technique in aquaculture breeding that involves directly selecting individuals with advantageous alleles or genotypes for specific traits using molecular markers closely associated with those traits, which applies the research findings of genomics and molecular biology to the selection and breeding of aquaculture species (Lu et al., 2019). It demonstrated that the construction and use of genetic linkage maps with a high number of molecular markers could connect desirable performance traits with molecular markers, which can benefit marker-assisted selection and breeding and accomplish genetic improvements for sea cucumber aquaculture. The accomplishments of marker-assisted selection and breeding can be evaluated through the correlation between performance traits and genetic information in offspring. In A. japonicus, the insertion/deletion (INDEL) marker of internal transcribed spacer 1 (ITS1) sequence divergence was successfully used to identify the genetic types of different body color varieties, and hybridization experiments showed that this marker could be used to distinguish and track the genetic composition of F1 hybrid larvae, which explored the relationship between body color variation and genetic background and its impact on larval development (Yoshida et al., 2012). The linkage map using AFLP markers serves as a foundation for constructing high-resolution genetic maps and identifying functional and quantitative trait loci (Li et al., 2009). Yan et al. constructed a common genetic map for sea cucumbers based on microsatellite and SNP markers, which was consistent with the haploid chromosome number of sea cucumbers (Yan et al., 2013). Liu et al. used a high-density genetic linkage map of sea cucumbers to preliminarily locate QTL regions associated with five traits: body length, body width, body weight, total number of spines, and survival rate (Liu et al., 2019). The “Sea Cucumber Superior No.1” was successfully bred using molecular marker-assisted breeding, which has strong resistance to Vibrio splendidus infection and fast growth rate (Ding and Chang, 2020). The new germplasm was achieved through population selection, screening and verification of disease-resistant molecular markers that indicate disease-resistant functional genes (Ding and Chang, 2020). In A. japonicus, a new high-density genetic linkage map was constructed based on single nucleotide polymorphism (SNP) molecular markers, and quantitative trait loci (QTL) mapping analysis was performed to explore the genetic mechanism of sea cucumber growth traits, which could be helpful for future marker-assisted breeding (Cui et al., 2021).
Molecular marker technology has diverse applications in fisheries research and offers significant convenience and solutions to numerous challenges encountered in the aquaculture industry. In order to tackle the obstacles faced by the sea cucumber aquaculture and breeding industry, various molecular markers have been developed and implemented for multiple purposes, including germplasm resource evaluation, species identification, Assessment of genetic diversity and population structure, and marker-assisted selection and breeding. With innovations in genomic methods, molecular markers are expected to continue playing important roles in promoting the future development of sea cucumber fisheries, aquaculture, and industry.
Author contributions
XL: Investigation, Visualization, Data curation, Writing – original draft. XT: Investigation, Visualization, Data curation, Writing – original draft. MC: Supervision, Resources, Conceptualization, Writing – review & editing. GN: Supervision, Resources, Conceptualization, Writing – review & editing. YY: Visualization, Supervision, Resources, Conceptualization, Writing – review & editing, Writing – original draft.
Funding
The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was supported by National Key Research and Development Program of China (grant number 2022YFD2401305).
Conflict of interest
The authors declare the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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Keywords: sea cucumber, molecular marker, aquaculture, germplasm conservation, molecular breeding
Citation: Liu X, Tang X, Chen M, Ni G and Yang Y (2024) Development and application of molecular markers in fisheries, aquaculture, and industry of representative temperate and tropical sea cucumbers: a review. Front. Mar. Sci. 11:1423096. doi: 10.3389/fmars.2024.1423096
Received: 25 April 2024; Accepted: 06 June 2024;
Published: 25 June 2024.
Edited by:
Arnold Rakaj, University of Rome Tor Vergata, ItalyReviewed by:
Chryssanthi Antoniadou, Aristotle University of Thessaloniki, GreeceTing Chen, Chinese Academy of Sciences (CAS), China
Copyright © 2024 Liu, Tang, Chen, Ni and Yang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Yujia Yang, yangyujia@ouc.edu.cn
†These authors have contributed equally to this work