Immunological characteristics of a recombinant alphaherpesvirus with an envelope-embedded Cap protein of circovirus
- 1MOA Key Laboratory of Animal Virology, Zhejiang University Center for Veterinary Sciences, Hangzhou, China
- 2College of Veterinary Medicine, Yangzhou University, Yangzhou, China
- 3State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, Zhejiang University, Hangzhou, China
A Corrigendum on
Immunological characteristics of a recombinant alphaherpesvirus with an envelope-embedded Cap protein of circovirus
By Lu C, Li H, Chen W, Li H, Ma J, Peng P, Yan Y, Dong W, Jin Y, Pan S, Shang S, Gu J and Zhou J (2024). 15:1438371. doi: 10.3389/fimmu.2024.1438371
In the published article, there was an error. In the Materials and Methods section, we conducted Cas9 editing on the homologous right arm region, US9 and US2, and the final site was US2 instead of US9.
A correction has been made to Materials and Methods, Construction for Cap protein of PCV2 transfer and US9 CRISPR-Cas9 gene editing vectors. This section sub-heading previously stated: “Construction for Cap protein of PCV2 transfer and US9 CRISPR-Cas9 gene editing vectors”.
The corrected sub-heading appears below:
“Construction for Cap protein of PCV2 transfer and US2 CRISPR-Cas9 gene editing vectors”.
In the published article, there was an error in Figure 1A as published. As stated above, we conducted Cas9 editing on the homologous right arm region, US9 and US2, and the final site was US2 instead of US9. The corrected Figure 1A and its caption appear below.
Figure 1. Generation of recombinant PRV with the Cap protein of PCV2. (A) The construction strategy of PRV-Cap. The PCV2 Cap gene was inserted into the gE extracellular region of parent PRV HD/c virus strain by homologous recombinant transfer vector, and then the fluorescent labeled EGFP gene was removed in vitro by the Cre-LoxP recombinant enzyme system to obtain the recombinant PRV with only the exogenous Cap gene. (B) IFA and (C) Western blotting assays of Cap-gE fusion protein expression in Vero cells inoculated with 1 MOI of PRV-Cap-EGFP, PRV-Cap, and PRV HD/c virus at 24 h post infection (D)Identification of inserting the Cap gene in Cap-EGFP and PRV-Cap by nucleic acid electrophoresis using gE-US7-9 primers.
The authors apologize for these errors and state that they do not change the scientific conclusions of the article in any way. The original article has been updated.
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Keywords: chimeric pseudorabies virus, circovirus, immunity, memory responses, IFN-γ
Citation: Lu C, Li H, Chen W, Li H, Ma J, Peng P, Yan Y, Dong W, Jin Y, Pan S, Shang S, Gu J and Zhou J (2024) Corrigendum: Immunological characteristics of a recombinant alphaherpesvirus with an envelope-embedded Cap protein of circovirus. Front. Immunol. 15:1515309. doi: 10.3389/fimmu.2024.1515309
Received: 22 October 2024; Accepted: 04 November 2024;
Published: 18 November 2024.
Edited and Reviewed by:
Wei Wang, Wenzhou University, ChinaCopyright © 2024 Lu, Li, Chen, Li, Ma, Peng, Yan, Dong, Jin, Pan, Shang, Gu and Zhou. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Jiyong Zhou, anl6aG91QHpqdS5lZHUuY24=