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ORIGINAL RESEARCH article

Front. Immunol.
Sec. Inflammation
Volume 15 - 2024 | doi: 10.3389/fimmu.2024.1475195
This article is part of the Research Topic Community Series in Targeting Signalling Pathways in Inflammatory Diseases - Volume II View all 10 articles

Integrative RNA, miRNA and 16S rRNA Sequencing Reveals Immune-Related Regulation Network for Glycinin-Induced Enteritis in Hybrid Yellow Catfish, Pelteobagrus fulvidraco ♀ × Pelteobagrus vachelli ♂

Provisionally accepted
Linyuan Yi Linyuan Yi Aijie Mo Aijie Mo *Huijun Yang Huijun Yang *Yifan Yang Yifan Yang *Qian Xu Qian Xu *Yong-chao Yuan Yong-chao Yuan *
  • College of Fisheries, Huazhong Agricultural University, Wuhan, China

The final, formatted version of the article will be published soon.

    Glycinin-induced foodborne enteritis is a significant obstacle that hinders the healthy development of the aquatic industry. Glycinin causes growth retardation and intestinal damage in hybrid yellow catfish (Pelteobagrus fulvidraco ♀ × Pelteobagrus vachelli ♂), but the immune mechanisms are largely unknown. In current study, five experimental diets containing 0% (CK), 1.74% (G2), 3.57% (G4), 5.45% (G6), and 7.27% (G8) immunological activity of glycinin were fed to juvenile hybrid yellow catfish for revealing the mechanism of the intestinal immune response to glycinin through RNA and miRNA sequencing, and exploring the interrelation between immune molecules and intestinal microbiota. The results demonstrated that glycinin content in posterior intestine increased significantly and linearly with the rise of dietary glycinin level. More than 5.45% of dietary glycinin significantly reduced the nutritional digestion and absorption function of posterior intestine. Notably, an obvious alteration in the expression levels of inflammatory genes (tnf-α, il-1β, il-15, tgf-β1) of posterior intestine was observed when dietary glycinin exceeded 3.57%. Sequencing results of RNA and miRNA deciphered 4246 differentially expressed genes (DEGs) and 28 differentially expressed miRNAs (DEmiRNAs) between the CK and G6 groups. Furthermore, enrichment analysis of DEGs and DEmiRNA target genes exhibited significant responses of the MAPK, NF-κB and WNT pathways following experiment fish exposure to 5.45% dietary glycinin. Additionally, at the level of 3.57% in the diet, glycinin obviously inhibited the increase of microbiota, especially potential probiotics such as Ruminococcus bromii, Bacteroides plebeius, Faecalibacterium prausnitzii and Clostridium clostridioforme. In sum, 5.45% dietary glycinin trough MAPK/NF-κB/WNT pathway to induce enteritis, and inflammatory condition could disrupt micro-ecological equilibrium through miRNA secreted by host in hybrid yellow catfish. This study constitutes a comprehensive transcriptional perspective of how the intestinal immunity respond to excessive glycinin in fish intestine.

    Keywords: Hybrid yellow catfish, glycinin, foodborne enteritis, intestinal immunity, Intestinal microbiata

    Received: 03 Aug 2024; Accepted: 16 Dec 2024.

    Copyright: © 2024 Yi, Mo, Yang, Yang, Xu and Yuan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Aijie Mo, College of Fisheries, Huazhong Agricultural University, Wuhan, China
    Huijun Yang, College of Fisheries, Huazhong Agricultural University, Wuhan, China
    Yifan Yang, College of Fisheries, Huazhong Agricultural University, Wuhan, China
    Qian Xu, College of Fisheries, Huazhong Agricultural University, Wuhan, China
    Yong-chao Yuan, College of Fisheries, Huazhong Agricultural University, Wuhan, China

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