Valentin Wenger1
Robert Zeiser1,2,3*- 1Department of Medicine I, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany
- 2Signaling Research Centre for Biological Signalling Studies (BIOSS) Freiburg and CIBSS – Centre for Integrative Biological Signalling Studies, University of Freiburg, Freiburg, Germany
- 3Comprehensive Cancer Center Freiburg (CCCF), Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany
Editorial on the Research Topic
Current concepts of cellular and biological drugs to modulate regulatory T cell activity in the clinic, volume II
Regulatory T cells (Tregs) represent a group of specialized CD4+ T cells that play a pivotal role in the maintenance of immune homeostasis and tolerance. In several diseases, however, the presence of Tregs interferes with a desirable effect to combat disease. This is specifically important in malignancy where Tregs have been shown to reduce the anti-tumor effects of immune checkpoint inhibitor (ICI) such as anti-PD-(L)1 (programmed cell death 1 (ligand) protein 1 or anti-CTLA-4 (cytotoxic T-lymphocyte-associated protein 4) and thereby promote secondary resistance. On the other hand, a lack of functional Tregs may enhance autoimmune diseases, such as rheumatoid arthritis, systemic lupus erythematosus, severe allergy, and allogeneic immune responses such as allograft rejection or graft-versus-host disease (GvHD).
In this editorial, the authors cover recent advances on Tregs including the role of glycoprotein A repetitions predominant (GARP), ectonucleotidases CD39 and CD73, as well as nanodrugs targeting T cells.
Tregs exert immunosuppression via multiple mechanisms including suppression of antigen-presenting cells (APCs), secretion of anti-inflammatory cytokines, reprogramming of metabolic functions, and direct cytotoxic effect.
Eschborn et al. elucidated on the role of the Transforming Growth Factor β (TGFβ)- activating transmembrane GARP. They demonstrated that depletion of GARP+ Tregs in food allergy results in increased gastrointestinal inflammation (1). Interestingly, Krebs et al. reported that patients with melanoma who did not respond to ICI had a longer survival if they expressed low frequency of GARP+ Tregs (2). In addition, Satoh et al. have described that the anti-GARP antibody DS-1055a is an effective treatment in a humanized mouse model of colorectal cancer (3). On the other hand, Wang et al. highlighted that GARP+ Tregs attenuate GvHD (4).
Another mechanism of action for Tregs’ suppressive functions is their ectonucleotide enzymes CD39 and CD73 that convert ATP to ADP/AMP and adenosine conversely, conferring local and short-lasting immune suppression. Allard et al. reported that CD73 has been upregulated in several tumor entities, such as ovarian, colon, breast cancer, and leukemia, and concluded that CD73 expression increases the risk for metastasis and chemoresistance (5). In addition, Allard et al. also reported that, by anti-CD73 monoclonal antibody treatment, effector T cells (Teff) and APCs potentiate Th1 response and IFNγ production due to the synergistic effect of CD73 and PD1 blockage (6). In a head and neck cancer model, Deng et al. demonstrated that CD73-blockade reduced tumor growth and reversed exhausted CD4+ and CD8+ T-cell phenotype (7). The role of CD73 in GvHD and anti-tumor immune responses has been reported in the past. Tsukamoto et al. revealed that pharmacological inhibition of CD73 resulted in enhanced graft-versus-tumor response (8).
A major hurdle for targeting Tregs in autoimmune diseases and cancer remains the difficulty to specifically target these cells. Therefore, the use of nanodrug carriers (NCs) has shown to be a promising approach, thereby maximizing on-target while minimizing any off-target effects. NCs targeting Teff and Tregs are an especially useful tool as they can be used for depletion/reprogramming of immunosuppressive cells, and APCs mediated T- cell activation or enhancement of immunogenic cell death. One such approach is the use of protein-based nanodrug (Nab-paclitaxel) that exhibited rapid tumor response for patients with metastatic breast cancer (9). Other groups demonstrated that polymer-based doxorubicin (Doxil) depleted myeloid-derived suppressor cells and increased cancer cell susceptibility to granzyme B while reducing cardiotoxicity (10–12).
In summary, this editorial provides an overview over several novel interventions employing a variety of therapeutic moieties that result in therapeutic up- or down-modulation of Treg function. Future studies will reveal whether some of these modalities are effective and safe enough to become part of our treatment armamentarium to combat autoimmunity and cancer.
Author contributions
All authors listed have made a substantial, direct, and intellectual contribution to the work and approved it for publication.
Funding
This study was supported by the Deutsche Forschungsgemeinschaft, Germany, SFB-1479 – Project ID: 441891347 (P01 to RZ) and SFB1160 (RZ).
Conflict of interest
RZ received honoraria from Novartis, MNK, Incyte, VectivBio, and Sanofi.
The remaining author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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References
1. Eschborn M, Weigmann B, Reissig S, Waisman A, Saloga J, Bellinghausen I. Activated glycoprotein a repetitions predominant (GARP)-expressing regulatory T cells inhibit allergen-induced intestinal inflammation in humanized mice. J Allergy Clin Immunol (2015) 136(1):159–68. doi: 10.1016/j.jaci.2015.04.020
2. Krebs FK, Trzeciak ER, Zimmer S, Özistanbullu D, Mitzel-Rink H, Meissner M, et al. Immune signature as predictive marker for response to checkpoint inhibitor immunotherapy and overall survival in melanoma. Cancer Med (2021) 10(5):1562–75. doi: 10.1002/cam4.3710
3. Satoh K, Kobayashi Y, Fujimaki K, Hayashi S, Ishida S, Sugiyama D, et al. Novel anti-GARP antibody DS-1055a augments anti-tumor immunity by depleting highly suppressive GARP+ regulatory T cells. Int Immunol (2021) 33(8):435–46. doi: 10.1093/intimm/dxab027
4. Wang H, Song H, Pham AV, Cooper LJ, Schulze JJ, Olek S, et al. Human LAP(+)GARP(+)FOXP3(+) regulatory T cells attenuate xenogeneic graft versus host disease. Theranostics (2019) 9(8):2315–24. doi: 10.7150/thno.30254
5. Allard B, Turcotte M, Stagg J. CD73-generated adenosine: orchestrating the tumor-stroma interplay to promote cancer growth. J BioMed Biotechnol (2012) 2012:485156. doi: 10.1155/2012/485156
6. Allard B, Pommey S, Smyth MJ, Stagg J. Targeting CD73 enhances the antitumor activity of anti-PD-1 and anti-CTLA-4 mAbs. Clin Cancer Res (2013) 19(20):5626–35. doi: 10.1158/1078-0432.CCR-13-0545
7. Deng WW, Li YC, Ma SR, Mao L, Yu GT, Bu LL, et al. Specific blockade CD73 alters the "exhausted" phenotype of T cells in head and neck squamous cell carcinoma. Int J Cancer (2018) 143(6):1494–504. doi: 10.1002/ijc.31534
8. Tsukamoto H, Chernogorova P, Ayata K, Gerlach UV, Rughani A, Ritchey JW, et al. Deficiency of CD73/ecto-5'-nucleotidase in mice enhances acute graft-versus-host disease. Blood (2012) 119:4554–64. doi: 10.1182/blood-2011-09-375899
9. Ciruelos E, Jackisch C. Evaluating the role of nab-paclitaxel (Abraxane) in women with aggressive metastatic breast cancer. Expert Rev Anticancer Ther (2014) 14(5):511–21. doi: 10.1586/14737140.2014.883922
10. Alizadeh D, Trad M, Hanke NT, Larmonier CB, Janikashvili N, Bonnotte B, et al. Doxorubicin eliminates myeloid-derived suppressor cells and enhances the efficacy of adoptive T-cell transfer in breast cancer. Cancer Res (2014) 74(1):104–18. doi: 10.1158/0008-5472.CAN-13-1545
11. Ramakrishnan R, Assudani D, Nagaraj S, Hunter T, Cho HI, Antonia S, et al. Chemotherapy enhances tumor cell susceptibility to CTL-mediated killing during cancer immunotherapy in mice. J Clin Invest (2010) 120(4):1111–24. doi: 10.1172/JCI40269
Keywords: regulatory T (Treg) cells, graft-versus host disease, CD73, GARP, anti-tumor immune response, autoimmune diseases
Citation: Wenger V and Zeiser R (2023) Editorial: Current concepts of cellular and biological drugs to modulate regulatory T cell activity in the clinic, volume II. Front. Immunol. 14:1221904. doi: 10.3389/fimmu.2023.1221904
Received: 13 May 2023; Accepted: 01 June 2023;
Published: 13 June 2023.
Edited and Reviewed by:
Magali Terme, Université de Paris, FranceCopyright © 2023 Wenger and Zeiser. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Robert Zeiser, cm9iZXJ0LnplaXNlckB1bmlrbGluaWstZnJlaWJ1cmcuZGU=