In the original article, there was a mistake in Figures 1, 3, 4, 5, 6 and their respective legends as published. The original figures and figure legends, were mislabeled with “0.16 ppm thyme oil”. They should have been labeled with the correct label “0.16 ppb thyme oil” in all instances. The correct figures and figure legends appears below.
Figure 1
Figure 3
Figure 4
Figure 5
Figure 6
In the original article, there was a mistake in the Supplementary Material as published. Throughout the Supplementary Material “0.16 ppm thyme oil” should be corrected to “0.16 ppb thyme oil” in all instances. The correct files appear below.
In the original article, there was an error. Throughout the text it was mistakenly written that “0.16 ppm thyme oil” was utilized for experiments. It should be written “0.16 ppb thyme oil” for all experiments. The original article has been updated.
In addition, in the original article, there was an error. It was mistakenly written “0.06 ppm thymol” instead of “0.06 ppb thymol”: “We estimate that 0.16 ppb thyme oil used in this experiment may contain approximately 0.06 ppb (60 ppb) thymol (37% thymol in 0.16 ppb thyme oil corresponds to ~ 60 ppb)”.
A correction has been made to 4. Materials and Methods, 4.4. Honey Bee Diet Preparation: “Post-injection, honey bees were housed in modified deli containers for the duration of the study. Bees in the control group were fed 50% sucrose syrup only, whereas bees in treatment groups were fed sucrose syrup containing one of the following additives: 0.16 ppb thyme oil (Body wonders), Fumagilin-B®, fumagillin dicyclohexyl ammonium (Medivet Pharmaceuticals Ltd.) at manufacturer’s recommended dose of 25 ppm or a higher dose of 75 ppm, or clothianidin at the field relevant sublethal concentration of 1 ppb or near lethal dose of 10 ppb (130, 195, 196). Thyme oil contains 10% - 64% thymol (~37% average) depending on the plant species, geographical sources, and harvest season, which may affect the volatile composition of the plant (113, 116-118). We estimate that 0.16 ppb thyme oil used in this experiment may contain approximately 0.06 ppb thymol (37% thymol in 0.16 ppb thyme oil corresponds to ~ 0.06 ppb). For clothianidin treatments we utilized the commercially available Poncho® 600, which contains 48% of the active ingredient clothianidin. A working stock of 1000 ppb clothianidin was prepared by 1:10 serial dilutions in 50% sucrose solution, which was further diluted in 50% sucrose to prepare 1 ppb (i.e., 10 ul of 1000 ppb Poncho® 600) and 10 ppb (i.e., 100 ul of 1000 ppb Poncho® 600) clothianidin solutions. Honey bees were fed sucrose solution either alone or with additives using cage feeders that were made by putting two holes on each side of a 1.5 mL centrifuge tube; sucrose solution was checked daily and refilled as needed throughout the study.”
The authors apologize for these errors and state they do not change the scientific conclusions of the article in any way.
Publisher’s Note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Parekh F, Daughenbaugh KF and Flenniken ML (2022) Corrigendum: Chemical Stimulants and Stressors Impact the Outcome of Virus Infection and Immune Gene Expression in Honey Bees (Apis mellifera). Front. Immunol. 13:844882. doi: 10.3389/fimmu.2022.844882
Received
28 December 2021
Accepted
24 January 2022
Published
02 March 2022
Volume
13 - 2022
Edited and reviewed by
Humberto Lanz-Mendoza, National Institute of Public Health, Mexico
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This article was submitted to Comparative Immunology, a section of the journal Frontiers in Immunology
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.