Corrigendum: Spatial Organization and Recruitment of Non-Specific T Cells May Limit T Cell-Macrophage Interactions Within Mycobacterium tuberculosis Granulomas

A Corrigendum on:
Spatial Organization and Recruitment of Non-Specific T Cells May Limit T Cell-Macrophage Interactions Within Mycobacterium tuberculosis Granulomas

By Millar JA, Butler JR, Evans S, Grant NL, Mattila JT, Linderman JJ, Flynn JL and Kirschner DE (2021). Front. Immunol. 11:613638. doi: 10.3389/fimmu.2020.613638

In the original article, there was a mistake in Figure 1 as published. The original image included cell types not referenced in the paper and data from an analysis was not included in the paper. The corrected Figure 1 legend appears below.

“Immunohistochemistry analysis of four non-human primates (NHP) granulomas [shown in Panels (A–D)] examining spatial distributions of both T cells and macrophages, and also where they intersect. Four distinct, randomly chosen granuloma images with extracted cell distributions. Column 1 shows the immunohistochemically stained preparation for CD3 (green), CD11c+ macrophages (red), and nuclei (dark blue). White points represent Geographical Information Systems Technology (GIS) analyses of these images revealing cell locations for T cells (Column 2), macrophages (Column 3), and their intersections (Column 4), as follows. Rows represent four distinct granulomas. The data for the cell numbers in these granulomas are given in Table 2. On average, about 9.75% (median 8.6%, StDev is 4.5%) of T cells interacted with macrophages.”

In the original article, there was a mistake in Table 2 as published. The original image included data from an analysis of cell types not described in this publication and non-standard non-human primate identification numbers. The corrected Table 2 appears below.

For the four IHC images of granulomas analyzed in Figure 1, we used GIS to count the T Cells (CD3) and macrophages (CD11c), cell distributions and their ratios. We also identified where the two cell types intersected. Intersections are defined as cell boundaries that touched or overlapped on the IHC image.

Nicole L. Grant was not included as an author in the published article. The corrected Author Contributions Statement appears below.

“DK, JF, and JL designed the study. NLG, JTM, SE, and JF carried out the experiments. JAM and JB analyzed the data. JAM and DK drafted the manuscript. All authors contributed to the article and approved the submitted version.”

In the original article, there was an error. In the Methods section, the number of macaques that the granulomas in Figure 1 were obtained from and their weeks post-infection were incorrectly included, antibodies for a cell type not analyzed in this study were included, and a microscope that used for imaging was excluded. A correction has been made to Methods, Immunohistochemistry and Imaging, paragraph 1:

“Four randomly selected, formalin fixed paraffin embedded (FFPE) granulomas were derived from 3 cynomolgus macaques (Macaca fascicularis), necropsied at approx. 10-11 weeks post infection (Figures 1A–D), and were deparaffinized and antigen retrieval was performed as previously indicated (35). Granulomas were stained with cocktails of antibodies including polyclonal rabbit anti-CD3 (Agilent Technologies, Santa Clara, CA), IgG2a mouse anti-CD11c (clone 5D11; Leica Microsystems, Buffalo Grove, IL). Primary antibodies were labeled with fluorochrome-labeled secondaries including anti-isotype (IgG2a) specific antibodies (Jackson ImmunoResearch, West Grove, PA). Coverslips were mounted with Prolong Gold with DAPI (ThermoFisher Scientific) and the sections were imaged on an Olympus FluoView confocal microscope (Center Valley, PA) or Nikon e1000 epifluorescence microscope (Nikon Instruments, Melville, NY) with Nikon NIS Elements (Nikon Instruments).”

FIGURE 1

Figure 1 Immunohistochemistry analysis of four non-human primates (NHP) granulomas [shown in Panels (A–D)] examining spatial distributions of both T cells and macrophages, and also where they intersect. Four distinct, randomly chosen granuloma images with extracted cell distributions. Column 1 shows the immunohistochemically stained preparation for CD3 (green), CD11c+ macrophages (red), and nuclei (dark blue). White points represent Geographical Information Systems Technology (GIS) analyses of these images revealing cell locations for T cells (Column 2), macrophages (Column 3), and their intersections (Column 4), as follows. Rows represent four distinct granulomas. The data for the cell numbers in these granulomas are given in Table 2. On average, about 9.75% (median 8.6%, StDev is 4.5%) of T cells interacted with macrophages.

In the Results section, we included a statistic for a cell type not analyzed in this study. A correction has been made to Results, Hypothesis 2: Spatial Organization of Granulomas Affects the Ability of T Cells to Reach Macrophages and Thus Be Activated Via Antigen Presentation, paragraph 2:

“We found that T cell-macrophage interactions occurred for, on average, only about 9.75% of the T cells identified (median: 8.6%, StDev: 4.5%), for at least the four granuloma that we examined (See Table 2).”

TABLE 2

Table 2 Geographical Information Systems Technology (GIS) analysis identifies numbers of cells of two types, and numbers of contacts from four immunohistochemistry (IHC) granulomas.

The authors apologize for these errors and state that they do not change the scientific conclusions of the article in any way. The original article has been updated.

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