This article is a correction to:
Priming Is Dispensable for NLRP3 Inflammasome Activation in Human Monocytes In Vitro
Anna Gritsenko1
Shi Yu2Fatima Martin-Sanchez1Ines Diaz-del-Olmo1
Eva-Maria Nichols3
Daniel M. Davis1
David Brough2
Gloria Lopez-Castejon1*- 1Division of Infection, Immunity and Respiratory Medicine, Faculty of Biology, Medicine and Health, Lydia Becker Institute of Immunology and Inflammation, University of Manchester, Manchester Academic Health Science Centre, Manchester, United Kingdom
- 2Division of Neuroscience and Experimental Psychology, Faculty of Biology, Medicine and Health, Lydia Becker Institute of Immunology and Inflammation, University of Manchester, Manchester Academic Health Science Centre, Manchester, United Kingdom
- 3Adaptive Immunity Research Unit, GSK, Stevenage, United Kingdom
A Corrigendum on
Priming Is Dispensable for NLRP3 Inflammasome Activation in Human Monocytes In Vitro
By Gritsenko A, Yu S, Martin-Sanchez F, Diaz-del-Olmo I, Nichols E-M, Davis DM, Brough D and Lopez-Castejon G (2020). Front. Immunol. 11:565924. doi: 10.3389/fimmu.2020.565924
In the original article, there was an error in the section Experimental Procedures, Cell Culture and Treatments, Paragraph 2. The incorrect guide RNA oligonucleotide sequences were given for GSDMD knockout in THP-1 cells: “GSDMD knockout THP-1 cells were lentivirally generated using guide RNA oligonucleotides sequence 5′-CACCGCTGCAAGCTGGCCAGGTACC-3′ and 5′ AAACGGTACCTGGCCAGCTTGCAGC-3′ (22) by utilizing the lentiCRISPR v2 plasmid system. lentiCRISPR v2 was a gift from Feng Zhang (Addgene plasmid # 52961; http://n2t.net/addgene:52961; RRID : Addgene_52961)”.
A correction has been made to the above sentence, as follows: “GSDMD knockout THP-1 cells were lentivirally generated using guide RNA oligonucleotide sequences 5’-CACCGACCAGCCTGCAGAGCTCCAC-3’ and 5’-AAACGTGGAGCTCTGCAGGCTGGTC-3’ (22) by utilizing the lentiCRISPR v2 plasmid system. lentiCRISPR v2 was a gift from Feng Zhang (Addgene plasmid #52961; http://n2t.net/addgene:52961; RRID : Addgene_52961)”.
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
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Keywords: macrophage, inflammasome, NLRP3, priming, monocytes, IL-18, GSDMD
Citation: Gritsenko A, Yu S, Martin-Sanchez F, Diaz-del-Olmo I, Nichols E-M, Davis DM, Brough D and Lopez-Castejon G (2021) Corrigendum: Priming Is Dispensable for NLRP3 Inflammasome Activation in Human Monocytes In Vitro. Front. Immunol. 12:763899. doi: 10.3389/fimmu.2021.763899
Received: 24 August 2021; Accepted: 27 August 2021;
Published: 13 September 2021.
Edited and reviewed by:
Alessandra Mortellaro, San Raffaele Telethon Institute for Gene Therapy (SR-Tiget), ItalyCopyright © 2021 Gritsenko, Yu, Martin-Sanchez, Diaz-del-Olmo, Nichols, Davis, Brough and Lopez-Castejon. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Gloria Lopez-Castejon, gloria.lopez-castejon@manchester.ac.uk