Corrigendum: Generation of Powerful Human Tolerogenic Dendritic Cells by Lentiviral-Mediated IL-10 Gene Transfer

A Corrigendum on
Generation of Powerful Human Tolerogenic Dendritic Cells by Lentiviral-Mediated IL-10 Gene Transfer

By Comi M, Amodio G, Passeri L, Fortunato M, Santoni de Sio FR, Andolfi G, Kajaste-Rudnitski A, Russo F, Cesana L and Gregori S (2020). Front. Immunol. 11:1260. doi: .10.3389/fimmu.2020.01260

In the original article, there was a mistake: a duplication of one dot plot in Figure 8B as published. The corrected Figure 8 appears below.

FIGURE 8

Figure 8 In vitro characterization of murine DC^IL-10. Female Balb/c BM cells were differentiated into DC, transduced at day 2 with LV-GFP (DC^GFP) or LV-IL-10 (DC^IL-10), and activated with LPS (200 ng/ml) during the last 2 days of differentiation. (A) Transduction efficiency was quantified based on ∆NGFR expression and the expression of the indicated markers was analyzed at day 9 of differentiation by flow cytometry. Each dot represents a single experiment (n = 8-9), lines indicate median, while whiskers are minimum and maximum levels. (B) DC were plated and left unstimulated or stimulated with LPS (200 ng/ml) for 24 h, with the addition of brefeldin A at 6 h. The expression of human IL-10 was quantified by intracytoplasmic staining. One representative donor out of two is depicted, and percentages of positive cells are indicated. (C) Spleen cells from female C57Bl/6 mice were stained with a proliferation dye and stimulated with Balb/c DC^GFP and DC^IL-10 at 1:10 ratio. At day 5, proliferation of CD4⁺ and CD8⁺ T cells was measured by flow cytometry. Each dot represents a single donor (n = 6), lines indicate median, while whiskers are minimum and maximum levels. *P ≤ 0.05 (Wilcoxon matched pairs test, two-tailed).

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.