Stefan Schülke- Vice President’s Research Group 1, Molecular Allergology, Paul-Ehrlich-Institut, Langen, Germany
Dendritic cells (DCs) are gatekeepers of the immune system that control induction and polarization of primary, antigen-specific immune responses. Depending on their maturation/activation status, the molecules expressed on their surface, and the cytokines produced DCs have been shown to either elicit immune responses through activation of effector T cells or induce tolerance through induction of either T cell anergy, regulatory T cells, or production of regulatory cytokines. Among the cytokines produced by tolerogenic DCs, interleukin 10 (IL-10) is a key regulatory cytokine limiting und ultimately terminating excessive T-cell responses to microbial pathogens to prevent chronic inflammation and tissue damage. Because of their important role in preventing autoimmune diseases, transplant rejection, allergic reactions, or in controlling chronic inflammation DCs have become an interesting tool to modulate antigen-specific immune responses. For the treatment of allergic inflammation, the aim is to downregulate allergen-specific T helper 2 (Th2) responses and the associated clinical symptoms [allergen-driven Th2 activation, Th2-driven immunoglobulin E (IgE) production, IgE-mediated mast cell and basophil activation, allergic inflammation]. Here, combining the presentation of allergens by DCs with a pro-tolerogenic, IL-10-producing phenotype is of special interest to modulate allergen-specific immune responses in the treatment of allergic diseases. This review discusses the reported strategies to induce DC-derived IL-10 secretion for the suppression of allergen-specific Th2-responses with a focus on IL-10 treatment, IL-10 transduction, and the usage of both whole bacteria and bacteria-derived components. Interestingly, while IL-10-producing DCs induced either by IL-10 treatment or IL-10 transduction are arrested in an immature/semi-mature state, treatment of DCs with live or killed bacteria as well as isolated bacterial components results in the induction of both anti-inflammatory IL-10 and pro-inflammatory, Th1-promoting IL-12 secretion often paralleled by an enhanced expression of co-stimulatory molecules on the stimulated DCs. By the secretion of DC-derived exosomes or CC-chemokine ligand 18, as well as the expression of inhibitory molecules like cytotoxic T lymphocyte-associated antigen 4, TNF receptor superfamily member 4, Ig-like transcript-22/cluster of differentiation 85, or programmed death-1, IL-10-producing DCs have been repeatedly shown to suppress antigen-specific Th2-responses. Therefore, DC-based vaccination approaches hold great potential to improve the treatment of allergic diseases.
Introduction
Dendritic Cells (DCs) Control the Induction of Immune Responses
Our immune system efficiently protects us from most pathogens. However, if the actions of immune cells are misdirected (e.g., against our own cells and tissues in the case of autoimmune diseases or against innocuous environmental antigens in the case of allergies) severe immunopathology can be the consequence. Therefore, the induction of potentially highly destructive immune responses needs to be tightly regulated.
Usually, the prevention of such detrimental immune responses is achieved by controlling against which antigens cells of the adaptive immune system are allowed to react. Here, antigen-presenting cells (APCs) are pivotal in controlling the induction of innate and subsequent adaptive immune responses.
Antigen-presenting cells consist of DCs, macrophages, and B cells (1, 2). They control both the induction and regulation of T-cell immune responses via the uptake, processing, and presentation of antigens to antigen-specific T cells (1, 2).
Among the different types of APCs, DCs are of special importance because they are the only APC type able to induce activation, differentiation, and expansion of naive, antigen-specific T cells (3, 4). In contrast to this, macrophages and B cells are only sufficient to reactivate T cells that have already encountered their specific antigen in the past (5).
Dendritic cells are highly specialized APCs strategically located in the skin and the mucosal system (2, 6). They act as sentinel cells that initiate, monitor, and regulate immune responses (1). In their immature form DCs continuously take up and process antigens via endocytosis or pinocytosis (7). If this antigen uptake occurs in the context of additional DC-activating signals such as pro-inflamatory cytokines [tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, or IL-6], prostaglandin hormones (prostaglandin E 2), immune stimulating bacterial and viral components [lipopolysaccharide (LPS), CpG-DNA; Pam2CysK4, flagellin, etc.], or cell-contact-dependent signals [e.g., via cluster of differentiation (CD)40-ligand] DCs become activated (8). Once activated, DCs start to present the processed antigens in the context of major histocompatibility complex II (MHC II) molecules and express co-stimulatory molecules on their surface (2, 8). Via the expression of the chemokine receptor 7 (CCR7, whose ligand is abundantly expressed in lymph nodes) mature DCs also start to migrate to lymph nodes, where DCs encounter antigen-specific naive T cells and initiate their priming (9, 10).
By their actions, DCs link innate and adaptive immune responses by connecting the detection of danger signals with the uptake, processing, and presentation of foreign antigens to control both the induction and polarization of primary antigen-specific CD4+ T-cell responses (11, 12).
Besides their important function in the induction of antigen-specific immune responses, DCs are also key players in maintaining immune homeostasis (13). Uptake and presentation of innocuous foreign- and self-antigens by DCs usually mediates T-cell tolerance (14). In this context, the cytokine IL-10 has been shown to shift DC function toward a tolerogenic rather than an immunogenic phenotype (15).
Dendritic cells may acquire tolerogenic properties either by (1) displaying a semi-mature state and exert tolerogenic function via the induction of apoptosis or anergy in the absence of co-stimulatory signals (2, 3, 16) promoting the differentiation of interacting T cells into CD4+CD25+ regulatory T (Treg) cells, or (3) increasing IL-10 production to expand allergen-specific type 1 regulatory T (Tr1) cells (3, 17, 18). Indeed, the T cell skewing capacity of DCs largely depends on their cytokine pattern and expression of co-stimulatory molecules (19, 20).
Therefore, depending on their maturation/activation status, the molecules expressed on their surface, and their cytokine production DCs have been shown to elicit immune responses through either activation of effector T cells, induction of tolerance through regulatory T cells, or the induction of regulatory cytokines (6).
Because of their important role in the induction of both innate and adaptive immune responses, DCs have become an interesting tool to modulate antigen-specific immune responses (11, 21). In this context, their capacity to induce, modulate, and control T cell responses makes DCs an attractive adjuvant in vaccination settings that have the aim to either enhance inadequate immune responses for the treatment of infectious diseases and cancer or to attenuate exaggerated immune responses in conditions such as autoimmunity, allergy, transplant rejection, and chronic inflammation (11, 21).
IL-10 Is an Important Cytokine Limiting Excessive Immune Responses
As we have just seen, cytokines produced by DCs play a central role in controlling both the induction and polarization of primary antigen-specific T-cell responses.
Among the cytokines produced by DCs, IL-10 is a key regulatory cytokine limiting and ultimately terminating excessive T-cell responses to microbial pathogens to prevent chronic inflammation and tissue damage (15, 22). IL-10 can both be produced by and has pleiotropic effects on multiple cell types, including DCs, macrophages, B cells, natural killer cells, both Th1- and Th2 cells, CD4+CD25+ forkhead box protein 3 (Foxp3+) Treg cells, and keratinocytes (1, 23–25).
Interleukin-10, originally identified as an inhibitor of interferon gamma (IFN-γ) and IL-2 synthesis in Th2 cells (26), efficiently inhibits proliferative and cytokine responses in T cells (1) and was shown to mediate both immunological unresponsiveness and the suppression of immune reactions (27). At epithelial interfaces to the environment, including the skin, IL-10 prevents excessive immune responses to foreign antigens (25).
Indeed, a well-documented mechanism by which IL-10-producing DCs suppress allergic Th2-responses is the induction of allergen-specific CD4+CD25+Foxp3+ Treg cells (27–29). For example, Pacciani et al. reported that IL-10-producing DC can induce allergen-specific regulatory T cells suppressing proliferation and inflammatory cytokine production from Th2 cells of the same specificity from house dust mite-allergic patients (30). In line with this, Oh and colleagues showed that IL-10-secreting T cells in the airways were able to reduce Th2-type inflammation and airway hyperreactivity (AHR) (31). Therefore, the induction of IL-10- and transforming growth factor beta (TGF-β)-producing regulatory T cells by IL-10-producing DCs is an important mechanism to prevent excessive immune responses (32, 33).
Consequently, IL-10-deficient mice develop increased contact hypersensitivity (34), display spontaneous enterocolitis and other symptoms akin to Crohn’s disease (35), and develop exaggerated asthmatic and allergic responses (35).
Interleukin-10 signaling is transmitted through a heterotetrameric interleukin 10 receptor (IL-10R) which consists of two ligand-binding IL-10R alpha chains and two accessory, signal-transducing beta chains all belonging to the interferon receptor family (Figure 1) (23). The IL-10R α chain is expressed at high levels on both macrophages and DCs, whereas the IL-10R β chain is ubiquitously and constitutively expressed by all cell types (23).
Figure 1. Immune modulatory signaling in antigen-presenting cells (APCs) induced by binding of interleukin-10 (IL-10) to the IL-10 receptor. Activation of the IL-10/Janus kinase 1 (JAK1)/tyrosine kinase 2 (Tyk2)/signal transducer and activator of transcription 3 (STAT3) pathway in APCs results in the phosphorylation of STAT3 by the interleukin 10 receptor (IL-10R) complex and the subsequent translocation of STAT3 homodimers into the nucleus. There STAT3 homodimers bind to STAT-binding elements and drive the expression of STAT-3-responsive genes such as suppressor of cytokine signaling 3 (SOCS-3) and IL-1 receptor antagonist (IL-1RN): SOCS-3 efficiently inhibits mitogen-activated protein kinase (MAP kinase) activation, NFκB translocation into the nucleus, and the subsequent induction of pro-inflammatory gene expression, while the decoy protein IL-1RN suppresses pro-inflammatory signaling normally initiated by binding of IL-1β to its receptor. STAT3 activation also inhibits STAT6 activation and therefore the expression of IL-4/IL-13-responsive genes. By these events, IL-10 reduces the production of pro-inflammatory cytokines (IL-1β, IL-6, tumor necrosis factor alpha) and diminished expression of both major histocompatibility complex II and co-stimulatory molecules (CD80, CD83, CD86) on APCs. Black arrows: activation of the indicated signaling pathways, orange arrows: inhibition of the indicated signaling pathways, black dashed arrows: pathways normally induced by the indicated molecules that are inhibited in the presence of STAT3 activation. For more detailed information, see Section “IL-10 Is an Important Cytokine Limiting Excessive Immune Responses.”
Mechanistically, IL-10 inhibits the function of APCs, including macrophages and DCs, by downregulating their maturation status and reducing the associated production of pro-inflammatory cytokines (such as IL-1β, IL-6, or TNF-α), while increasing the expression of inhibitory genes (23, 36). These effects of IL-10 are mediated via the Janus kinase 1 (JAK1)/Tyk2/STAT3 pathway. It is initiated when IL-10 homodimers bind to the extracellular portion of the IL-10R alpha chain (Figure 1). In a first activation step, IL-10 binding triggers the recruitment of Jak1 to the IL-10R alpha chain and its subsequent phosphorylation, while tyrosine kinase 2 (Tyk2) is recruited to and phosphorylated by the IL-10R beta chain (Figure 1) (37, 38). Upon their phosphorylation, these kinases phosphorylate the tyrosine motifs Y446 and Y496 located in the intracellular portion of the IL-10R alpha chain (Figure 1) (39). The activated IL-10 receptor complex then mediates the phosphorylation of signal transducer and activator of transcription 3 (STAT3) by providing transient anchorage sites for STAT-3 that allow the phosphorylation of STAT3 by Jak1 and Tyk2 (39, 40). Phosphorylated STAT3 forms homodimers which translocate into the nucleus, bind to STAT-binding elements, and drive the expression of STAT-3-responsive genes. Among others, these include the suppressor of cytokine signaling 3 (SOCS-3) and the IL-1 receptor antagonist (IL-1RN) (Figure 1) (41): SOCS-3 subsequently inhibits mitogen-activated protein kinase activation, NFκB translocation into the nucleus, and the associated induction of pro-inflammatory gene expression (40). SOCS-3 also mediates Jak1-inhibition, resulting in feedback inhibition of the JAK1/Tyk2/STAT3 pathway (42).
In addition, production of IL-1RN, a decoy protein binding to the IL-1 receptor, blocks pro-inflammatory signaling normally initiated by binding of IL-1β to this receptor (Figure 1) (43).
Interleukin-10 also directly inhibits Th1 cell differentiation (by reducing IL-2, IL-12, and INF-γ production), limits effector T-cell function (by suppressing TNF-α, IL-1β, and IL-6 production), and promotes the development, expansion, and function of regulatory T cells (23, 44). For example, IL-10 is known to inhibit the expression of IL-4 and IL-13-responsive genes in monocytes and DCs by suppressing the activation of STAT6 (Figure 1) (45).
Interleukin-10/IL-10R signaling may also result in STAT1 and STAT5 phosphorylation in monocytes and Treg cells, but the interactions of STAT1 and STAT5 with other intracellular signaling events triggered by IL-10 are still unclear (42).
Because of its broadly anti-inflammatory effects, IL-10 is a highly interesting molecule for the treatment of allergic diseases, where affected patients mount exaggerated, immunoglobulin E (IgE)- and Th2-mediated immune responses against otherwise harmless environmental antigens.
In line with this, DC-derived autocrine IL-10 secretion was shown to suppress high-affinity IgE receptor Fc epsilon receptor I-dependent pro-inflammatory responses (46), suggesting that increased IL-10 production by DCs during allergy immunotherapy may reduce inflammatory responses to the allergen (47).
Up to now, numerous studies support the importance of IL-10 produced by either Treg or Tr1 cells (48, 49), IL-10-producing regulatory B cells (50), and lung DCs (4, 32, 51) in the modulation of allergic diseases. Among other findings, IL-10 production by murine lung DCs suppressed inflammation and promoted the establishment of allergen-specific tolerance (52). In line with its function in the suppression of lung inflammation, IL-10 expression has been reported in DCs located in both lung tissue and the intestine, suggesting IL-10 to fulfill an important role in maintaining local T-cell tolerance to common environmental antigens (32).
The importance of IL-10 in controlling allergic inflammation is further highlighted by its ability to decrease eosinophil survival and IgE synthesis (53, 54). Indeed, IL-10 is often regarded as a key cytokine mediating tolerance in patients undergoing immunotherapy (55, 56).
Allergic Patients Show a Tendency to Produce Reduced IL-10 Levels upon Allergen Contact
In line with the importance of IL-10 in suppressing allergic responses, Akbari and colleagues reported that DCs from mice exposed to harmless inhaled antigens transiently produce IL-10 stimulating the development of IL-10-secreting, antigen-specific CD4+CD25+Foxp3+ Tregs (32). Moreover, upon stimulation with the probiotic bacterium Escherichia coli 083, a lower expression and secretion of IL-10 was detected from monocyte-derived DCs (moDCs) derived from newborns of allergic mothers compared with cells derived from children with non-allergic mothers (57).
These results suggest that a reduced capacity to produce DC-derived IL-10 upon antigen contact may facilitate the development of allergic diseases by skewing immune responses toward the differentiation of Th2 cells and the development of childhood atopy and/or asthma (2).
Early studies showed that DC-derived IL-10 production is profoundly diminished in allergic rhinitis (AR) children regardless of the presence or absence of asthma, while DC-derived IL-12 secretion as well as T cell cytokine secretion were unchanged (2). These results suggest that atopic individuals have an intrinsic inability to upregulate DC-derived IL-10 production (2). In line with this, several studies reported diminished antigen-induced, peripheral blood mononuclear cell (PBMC)-derived IL-10 production in children (58, 59) and adults (31, 60, 61) with atopic disorders (AR, asthma, or atopic dermatitis).
In addition, several studies have reported that allergic patients show a tendency to produce reduced levels of IL-10 upon allergen exposure. For example, defects in IL-10-producing T cells have been implicated in the immunopathogenesis of airway allergy, resulting in Th2-mediated production of allergen-specific IgE and tissue eosinophilia (55, 62).
Moreover, Wei et al. reported that IL-10 levels in the supernatants of DCs from AR patients were significantly lower than those observed in healthy controls (63). Accordingly, Pilette and coworkers described not only local nasal DCs but also systemically circulating blood myeloid DCs (mDCs) from AR patients to exhibit reduced IL-10 and IL-12 expression after allergen provocation, while activated plasmacytoid DCs from these patients produced diminished amounts of interferon alpha (IFN-α) and triggered reduced levels of IL-10 from allogeneic CD4+ T cells (64). Due to these changes in cytokine production mDCs from AR patients preferentially supported Th2-cell polarization, linking systemic DC dysfunction to biased T-cell responses and the failure to regulate T-cell-mediated responses to allergens seen in atopic patients (64).
While most studies suggest allergic patients to produce reduced levels of IL-10 compared with healthy individuals, some groups report contrary results: Lied and coworkers reported LPS-stimulated moDCs from atopic patients to produce significantly more IL-10 compared to non-atopic patients (65) and Frischmeyer-Guerrerio and colleagues described that mDCs from food allergic children produced greater quantities of IL-10 (66).
Successful Allergen-Specific Immunotherapy May Restore Reduced IL-10 Secretion in Allergic Patients
Since several studies have reported that allergic patients show a tendency to produce reduced levels of IL-10 upon allergen exposure (see above paragraph), restoring allergen-induced IL-10 secretion from DCs is one of the aims in allergen-specific immunotherapy (AIT).
Indeed, IL-10 is often regarded as a key cytokine mediating tolerance in patients undergoing immunotherapy (55, 56). Many of the observed beneficial immune alterations during AIT have been attributed to IL-10 production (67). Several studies reported increased levels of IL-10 in blood and affected tissues of patients that underwent AIT (4, 68, 69). However, the cellular source of AIT-induced IL-10 production seems to depend on the exact treatment modalities: for example, exposure to high doses of allergen was repeatedly shown to result in the induction of different IL-10-producing CD4+CD25+Foxp3+ Treg subsets (70–72), while for patients receiving pollen AIT increased IL-10 production was reported in mucosal macrophages (69). Interestingly, in contrast to this, increased numbers of IL-10-producing B cells and monocytes were described in the peripheral blood of patients receiving bee venom AIT (70, 73).
While many of the beneficial effects of AIT-induced IL-10 production are attributed to the induction of allergen-specific, IL-10-producing CD4+CD25+Foxp3+ Tregs (69–72), other cellular sources of IL-10 should not be ignored. In line with this, Kunz and coworkers recently described that the IL-10-dependent induction of allergen-specific tolerance by subcutaneous allergen injection resulted in increased IL-10 signals in T and B cells of both skin draining and mediastinal lymph nodes (74). Interestingly, tolerance induction could still be achieved when mice were unable to produce either T cell-, B cell-, T and B cell-, or DC-derived IL-10 (74). In contrast to this, tolerance induction was not possible if all hematopoetic cells were unable to produce IL-10 (74). Taken together, these results suggest a high degree of functional cellular redundancy in IL-10-mediated tolerance induction (74).
Strategies to Induce DC-Derived IL-10 Secretion
We have seen that immature DCs can be used to as tolerogenic DCs to suppress antigen-specific T cell responses. A regulatory, IL-10-producing DC phenotype is of special interest for the treatment of allergic diseases where the treatment aim is to downregulate allergen-specific Th2 responses and the associated clinical symptoms (allergen-specific Th2-responses, IgE-mediated mast cell and basophil activation, allergic inflammation). The remainder of this review will summarize the strategies used to generate such IL-10-producing DCs with a focus on IL-10 treatment, IL-10 transduction, and the usage of both whole bacteria and bacteria-derived components (see also Figures 2 and 3 and Table 1). Besides these strategies, several other factors have been described to promote the differentiation of IL-10-producing DC subsets in the context of allergies. Among others, these are endothelial splenic stroma (11), aryl hydrocarbon receptors (63), the phosphodiesterase-4 inhibitor roflumilast (75), and dexamethasone (16). Also induction of cyclic adenosine monophosphate was shown to result in upregulated expression of DC-derived IL-10, attenuated secretion of the pro-inflammatory cytokines TNF-α and IL-12, and impaired T cell stimulation by these DCs (76).
Figure 2. Phenotype and immune modulatory effects of interleukin-10 (IL-10)-producing, semi-mature tolerogenic DCs. (A) Strategies resulting in the generation of IL-10-producing, semi-mature tolerogenic dendritic cells (DCs) with reduced expression of co-stimulatory molecules, cell adhesion molecules, and lower secretion of pro-inflammatory cytokines. Expression levels are indicated as follows: low: low expression, int: intermediate expression, high: high expression, +: positive for the indicated molecule; arrow up: increased production, arrow down: decreased production. (B) Immune modulatory effects of tolerogenic DCs displaying a semi-mature DC phenotype. The lack of co-stimulation and antigen presentation results in the preferred induction of either anergic or regulatory T cell subsets which themselves are able to suppress both Th1- and Th2-responses. Also, the reprogramming of CD4+ memory T cells into IL-10 and IL-4 co-producing Th0-like cells has been described.
Figure 3. Phenotype and immune modulatory effects of interleukin-10 (IL-10) producing, mature tolerogenic DCs co-producing pro-inflammatory cytokines. (A) Strategies using live or killed bacteria and bacterial components resulting in pro-tolerogenic dendritic cell (DC) phenotypes characterized by the expression of high levels of co-stimulatory molecules as well as the co-production of IL-10 and pro-inflammatory cytokines [IL-1β, IL-6, IL-12, tumor necrosis factor alpha (TNF-α)]. Expression levels are indicated as follows: low: low expression, int: intermediate expression, high: high expression, +: positive for the indicated molecule; arrow up: increased production, arrow down: decreased production. (B) Regulation of T cell responses by DCs stimulated with bacteria or bacterial components inducing an IL-10-positive DC phenotype that is characterized by the co-production of IL-12 and high expression levels of co-stimulatory molecules. In this context, both the induction of different regulatory T cell subsets and Th1-biased effector cells have been described. +: positive for the indicated molecule.
Table 1. Summary of the strategies reported in the literature to induce dendritic cell (DC)-derived interleukin-10 (IL-10) secretion.
IL-10 Treatment
The simplest strategy to induce tolerogenic IL-10 DCs is to differentiate naive DCs in the presence of IL-10. Indeed, several studies reported IL-10-treated human or mouse DCs to induce antigen-specific anergy (99–101).
Immunologically, T cell tolerization (meaning the induction of antigen-specific CD4+CD25+ Treg cells) by IL-10-treated DCs requires a partially activated DC status commonly referred to as semimaturation (102, 103). In contrast to this, complete DC activation likely is more immunogenic than tolerogenic, resulting in the activation of effector T cell subsets (102, 103). This semi-mature status is characterized by high-expression levels of MHC, intermediate to low levels of co-stimulatory molecules, and a strongly reduced production of pro-inflammatory cytokines such as IL-12 (Figure 2A) (102). Moreover, the development of antigen-specific CD4+CD25+Foxp3+ Treg cells, inhibiting allergic responses, was shown to be dependent on the presence of both IL-10 and an inducible co-stimulator (ICOS)–inducible co-stimulator ligand (ICOS-L) interaction provided by DCs (Figure 2B) (32).
Here, the suppression of Th2-responses by the induced regulatory T cells was repeatedly described to occur via a cell contact-dependent and antigen non-specific manner (48, 104, 105). For example, DCs treated with IL-10 were shown to induce CD4+ T cells expressing the cytotoxic T lymphocyte-associated antigen 4 (CD152, CTLA4), capable of mediating tolerance in a cell contact-dependent manner (Figure 2B) (99, 106). In addition, some studies reported an increased expression of inhibitory molecules such as the Ig-like transcript-22/CD85 (77) on the surface of IL-10-treated DCs (Figure 2A).
The exact tolerogenic potential of IL-10-treated DCs seems to dependent on the experimental model used: while there are some reports suggesting that DCs treated with IL-10 may increase the secretion of Th2 cytokines (while suppressing Th1-responses) (107, 108), the majority of studies have described an efficient suppression of both mouse and human Th1- and Th2-responses by DCs treated with IL-10 (101).
The potency of IL-10-treated DCs to prevent the development of lung allergic responses in mice was demonstrated by Koya and colleagues (1). Koya et al. reported IL-10-treated DCs to suppress production of the Th2 cytokines IL-4, IL-5, and IL-13 in vitro and decrease both AHR and airway inflammation in vivo (1). Here, transfer of ovalbumin (OVA)-pulsed, IL-10-treated DCs into naive mice prevented the development of AHR, airway eosinophilia, reduced Th2 cytokine levels in bronchoalveolar lavage (BAL) fluid, and goblet cell metaplasia when challenged with the allergen (1).
In their hands, the IL-10-treated DCs displayed a tolerogenic phenotype, expressing lower levels of CD11c, CD80, and CD86, while producing lower amounts of IL-12 but significantly more IL-10 (Figure 2A) (1). Mechanistically, this endogenous IL-10 production of exogenously IL-10-treated DCs was shown to be required for their regulatory function since DCs from IL-10-deficient mice did not display regulatory function even when differentiated in the presence of IL-10 (1).
In accordance with these results, Li et al. did report IL-10-treated human DCs to induce tolerance in autologous T cells of patients with asthma (77). Phenotypically, the IL-10-treated DCs expressed reduced levels of the co-stimulatory and maturation markers CD86, human leukocyte antigen DR, and CD54, only modest reductions in CD40 and CD80, and reduced levels of the pro-inflammatory cytokines IL-6 and IL-12 (Figure 2A) (77). In contrast to this, expression levels of Ig-like transcript-22/CD85j, IFN-α1, IL-2, and IL-10 were strongly increased (Figure 2A) (77). In this context, the inhibitory receptor Ig-like transcript-22/CD85j was shown to have an important role both in the regulation of natural killer cells and T cells (109, 110) and the function of tolerogenic DCs (110).
In co-culture with autologous CD4+ T cells IL-10-treated DCs inhibited Th2 cell differentiation and production of Th2-related cytokines (IL-4, IL-5, and IL-13) otherwise driven by immunostimulatory DCs differentiated in the presence of TNF-α (77). Moreover, treatment of DCs with IL-10 led to a significant outgrowth and activation of CD4+CD25+LAG-3+CTLA-4+ Foxp3+ IL-10-secreting Tr1-type Tregs, and resulted in allergen-specific induction of tolerance in a contact-dependent manner which was critically dependent on expression of IL-10 by DC (Figure 2B) (77).
Although the tolerogenic capacity of IL-10-treated DCs is well described, Bellinghausen et al. reported treatment of DCs with IL-10 alone (in contrast to the efficient suppression of Th1 responses by IL-10-treated DCs) to be insufficient for the suppression of Th2-responses (78). In their hands, the induction of regulatory T cells with the ability to suppress Th2 cytokine production required at least two signals: IL-10 plus TGF-β (78). In their experimental system, the suppressive capacity of the IL-10 plus TGF-β-induced regulatory T cells was shown to be antigen-unspecific and strongly dependent on both cell–cell contact and the surface molecule programmed death-1 (PD-1) (78). Interestingly, neutralization of either IL-10, CTLA-4, or TGF-β had only marginal effects on the suppressive capacity of the induced CD4+CD25+ Tregs (78). Here, incubation of T cells with IL-10 alone instead of IL-10-treated DC did not lead to the generation of inducible Tregs (iTregs), suggesting that additional signals provided by the tolerizing DC are necessary for the generation of iTregs (78).
One such factor might be DC-derived CC-chemokine ligand 18 (CCL18). When performing a genome-wide analysis of gene expression in co-cultures of CD4+ T cells from patients with grass pollen allergy and either tolerogenic, IL-10-treated DCs or regular, mature allergen-pulsed DCs, Bellinghausen and coworkers could show that in DCs differentiated in the presence of IL-10 the only gene being upregulated was CCL18 (while many genes were downregulated) (79). These IL-10-treated, CCL18-producing DCs efficiently suppressed IL-13, IL-5, and TNF receptor superfamily member 4 (OX40) gene expression in CD4 T cell:DC co-cultures (79). Of note, exogenous addition of CCL18 to these co-cultures was sufficient to induce a similar inhibition of Th2 cytokine production compared to allergen-pulsed, IL-10-treated DCs (without affecting IFN-γ or IL-10 production) (79). In these co-cultures, neutralizing IL-10 did not reduce CCL18 production suggesting that factors other than IL-10 are involved in maintaining the enhanced CCL18 expression in IL-10-treated DCs (79). In a humanized mouse model of airway allergy, application of CCL18 inhibited airway reactivity and lung inflammation, preferentially attracting regulatory T cells over Th2 cells (79). Therefore, CCL18 was shown to be an important effector molecule of tolerogenic IL-10-treated DCs.
IL-10 Transduction
Besides differentiating DCs in the presence of IL-10, several studies have described transduction of DCs with the IL-10 gene to result in DCs with tolerogenic properties. Here, IL-10-transduced DCs were shown to induce long-lasting, antigen-specific tolerance by induction of regulatory T cells (Figures 2A,B) (4).
Henry and coworkers reported a single intratracheal injection of OVA-pulsed, IL-10-transduced DCs to prevent eosinophilic airway inflammation, AHR, production of mucus, antigen-specific IgE and immunoglobulin G1 (IgG1) antibodies, and IL-4 as well as IFN-γ production in a mouse model of experimental asthma (Figure 2B) (4). These effects were shown to also depend on non-DC-derived IL-10 since IL-10-deficient mice treated with IL-10-transduced wild-type DCs were less well protected (4). Phenotypically, IL-10-transduced DCs displayed intermediate levels of cell surface maturation markers MHC II, CD40, CD80, and CD86 and secreted high amounts of IL-10, but no IL-12 (Figure 2A) (4). In contact with allergen-specific T cells, these semi-mature DCs induced both differentiation and proliferation of antigen-specific CD4+CD25+Foxp3+ IL-10-producing regulatory T cells in the mediastinal lymph nodes of animals treated with IL-10-transduced DCs (4). These effects were shown to be antigen-specific, since IL-10-transduced DCs, primed with the major house dust mite allergen Dermatophagoides pteronyssinus peptidase 1 (Der p 1), did not protect against OVA-induced airway allergy (4).
In line with these results, Nakagome et al. reported IL-10 gene delivery by plasmid transfer to suppress OVA-induced eosinophilic airway inflammation and AHR, suppressing the overall function of CD11c+ lung APCs in terms of antigen-presenting capacity, cytokine production, and transport of antigen to lymph nodes resulting in reduced Th2 responses (80).
In an attempt to optimize DC-derived IL-10 transduction, Besche et al. showed that the usage of the DC-specific fascin promoter for IL-10 overexpression in bone marrow-derived DCs (BM-DCs) to result in the generation of IL-10+IL-6+ DCs with reduced IL-12p40 mRNA expression (3). In vivo application of these IL-10-transduced BM-DCs efficiently inhibited ear swelling responses in a mouse model of hapten-induced contact hypersensitivity (3).
Exosomes from IL-10-Treated DCs
Besides secreting immune modulatory cytokines, IL-10-treated DCs were also shown to secret exosomes with immune modulating capacity involved in the suppression of inflammatory and autoimmune responses (Figure 2A) (81).
Kim et al. reported exosomes isolated from either BM-DCs transduced ex vivo with an adenovirus expressing the IL-10 gene or BM-DCs treated with recombinant murine IL-10 protein to express high levels of MHC II, moderate levels of MHC I, CD11c, CD80, and CD86 on their surface (Figure 2A) (81). Upon periarticular administration, these exosomes were shown to suppress delayed-type hypersensitivity responses within both injected and untreated contralateral joints, while systemic injection suppressed the onset of murine collagen-induced arthritis and reduced the severity of established arthritis in a mouse model (Figure 2B) (81). Here, administration of isolated exosomes had comparable effects to the application of IL-10 transduced DC (81). Mechanistically, the suppressive capacity of the exosomes was shown to depend on surface expression of MHC II (81). The authors speculated that these exosomes may be able to bind and possibly fuse with endogenous cells (macrophages, or APCs) to subsequently modulate their activity (81).
These results were confirmed by Ruffner and colleagues which demonstrated that IL-10 treatment generates both DCs with a pro-tolerogenic phenotype and a population of immunosuppressive exosomes (82). Treatment of DC with IL-10 significantly downregulated surface expression of MHC I, MHC II, CD80, CD86, and programmed death ligand 2 (PD-L2) (Figure 2A) (82). In addition to the modified co-stimulatory profile of IL-10-treated DCs, exosomes derived from these DCs were shown to also contain reduced surface levels of CD80, PD-L1, and PD-L2 (Figure 2A) (82). Here, the suppressive capacity of both IL-10-treated DCs and exosomes derived from these cells in a mouse model of delayed-type hypersensitivity was shown to depend on CD80 and CD86, but not PD-L1 and PD-L2 expression (82).
Live Bacteria
Several studies have described the potential of different bacteria to induce DC-derived IL-10 production (27, 84). Among others, E. coli 083 (57), Helicobacter pylori (83), Clamydia (27), Listeria monocytogenes (85), Mycobacterium vaccae (111), and Bacillus Calmette–Guérin (BCG) (84) were reported to induce DC-derived IL-10 secretion with immune modulatory potential for the treatment of allergic diseases. Of note, in contrast to IL-10-treated DCs, many of the available studies suggest that stimulation with either bacteria (live or killed) or isolated bacterial components induces both anti-inflammatory IL-10- and pro-inflammatory, Th1-promoting IL-12 secretion often alongside an enhanced expression of co-stimulatory molecules on the stimulated DCs (Figure 3A) (27, 84, 86, 98, 112).
Here, IL-10 secretion induced by bacteria can either prevent excessive inflammatory responses or suppress immune responses otherwise directed against the bacterium (27). In theory, this IL-10 induction by bacteria may be used to modulate immune responses in the host to unrelated antigens such as allergens (27).
In line with this, epidemiological and experimental studies revealed a strong inverse relationship between chronic H. pylori infection (which induces IL-10 secretion from DCs) and the risk of developing allergic asthma, hay fever, or eczema (113–115). Here, Engler and colleagues further investigated the mechanism underlying the protective effects of H. pylori (83). They reported extracts of H. pylori to prevent allergen-induced airway hyperresponsiveness, bronchoalveolar eosinophilia, pulmonary inflammation, and Th2 cytokine production in a mouse asthma model (Figure 3B) (83). Mechanistically, this suppression of Th2-responses was shown to require a heat-sensitive H. pylori component (possibly the H. pylori persistence determinants γ-glutamyl-transpeptidase GGT and the vacuolating cytotoxin VacA) and the production of IL-10 by basic leucine zipper ATF-like 3 (BATF3)-dependent CD103 and CD11b positive DCs infiltrating the lungs of protected animals (Figure 3A) (83). Moreover, both IL-18 and BATF3 were critically required for H. pylori-mediated protection against allergic responses (83). In contrast to this, suppression of Th2-responses was independent of regulatory T cells since antibody-mediated depletion of CD25+ Tregs had no effect on the suppression of Th2-responses (83). Interestingly, in vitro BM-DC-derived IL-10 secretion induced by H. pylori extracts was shown to depend on myeloid differentiation primary response 88 (MyD88) and toll-like receptor (TLR)2- but not TLR4-signaling (83).
Súkeníková et al. reported E. coli 083 to also induce increased gene expression and secretion of IL-10 in DC of newborns of healthy mothers in comparison to DCs derived from newbornes from allergic mothers (57). This higher IL-10 production was associated with lower levels of IL-4, IL-13, IFN-γ, IL-17A, and IL-22 in DC:CD4+ T cell co-cultures (Figure 3B) (57).
In line with these results, infection of mouse DCs with BCG resulted in a significant enhancement of both IL-10 and IL-12 production (84). Interestingly, BCG-stimulated DCs were characterized by a higher surface expression of CD8a, co-stimulatory molecules CD80, CD86, and CD40, and TLRs (Figure 3A) (84). Here, adoptive transfer of DCs from BCG-infected mice, but not DCs from naive mice, significantly inhibited established allergic airway eosinophilia, mucus overproduction, IgE production, and Th2 cytokine production (Figure 3B) (84). These protective effects of BCG-infected DCs were reversed by the application of either IL-10- or IL-12-neutralizing antibodies, showing both cytokines to be involved in the suppression of the allergic response (84).
Stock et al. described L. monocytogenes to induce CD8a+ DCs co-producing both IL-10 and IL-12 (Figure 3A) (85). These DCs mediated the differentiation of ICOS+Foxp3+ T-box transcription factor TBX21 (T-bet)+ Th1-like CD4+CD25+ Treg cells that themselves produced both IL-10 and the Th1 cytokine IFN-γ (Figure 3B) (85). Therefore, these cells combined features of both regulatory T cells and Th1 cells (85).
Han et al. reported the adoptive transfer of CD8+ICOS ligand (ICOS-L)+IL-10+IL-12+ DCs isolated from Chlamydia-infected mice (Figure 3A), but not those from naive mice, to inhibit OVA-induced systemic and cutaneous eosinophilia after intranasal challenge with OVA (27). In vitro DCs from Chlamydia-infected mice were shown to inhibit allergen-specific Th2 cell differentiation while promoting Th1 responses in an IL-10-, IL-12-, and ICOS-L-dependent way (Figure 3B) (27).
Taken together, these results show that in addition to inducing Th1-priming DCs, infection with different bacteria can result in the differentiation of tolerogenic, IL-10-producing DC subsets characterized by both high expression levels of co-stimulatory molecules and the co-production of pro-inflammatory cytokines (Figure 3A). Mechanistically, these more activated tolerogenic DCs were shown to suppress allergen-specific Th2-responses via the induction of either Th1, Th1-like Tregs, CD4+CD25+Foxp3+ Treg, or Tr1 cells (Figure 3B).
Aside from results obtained with live bacteria, also bacterial extracts and heat killed bacteria were described to suppress allergen-specific Th2-responses. Here, both IL-10-dependent and -independent mechanisms of immune modulation were described.
For example, Pochard et al. reported the addition of heat killed E. coli to peanut-pulsed DCs to suppress both peanut-induced secretion of the Th2 cytokines IL-4, IL-5, IL-13, and T cell proliferation while increasing IFN-γ production in a MyD88/TIR-domain-containing adapter-inducing interferon-β-dependent manner (86). Although stimulation of the DCs with heat killed E. coli did trigger DC-derived, TLR4-dependent IL-10 secretion, these effects of were not influenced by neutralization of IL-10 but shown to be dependent of IL-12/IL23 p40 and IFN-γ secretion (86). Therefore, the suppression of Th2-responses described by Pochard et al. is mediated by a TLR-mediated enhancement of Th1-responses, which in turn downregulate Th2-responses via IL-12 production (86).
Bacterial Components
Besides whole bacteria and bacterial extracts, which are complex mixtures of different, potentially immune modulating components, some single bacterial components have been investigated for their DC tolerizing potential in the context of allergies (Figure 3A). For differentiation purposes, these components will be divided into TLR-ligands and non-TLR-ligands.
TLR Ligands
Bacteria- and virus-derived pathogen-associated molecular patterns are sensed by pathogen-recognition receptors and induce innate and subsequent adaptive immune responses. Due to their intrinsic capacity to activate innate immune cells, TLR ligands are interesting immune modulating components for the treatment of allergic diseases. Here, TLR2-, TLR3-, TLR4-, and TLR5-ligands have been described to induce tolerogenic DC subsets.
Bacterial lipopeptides such as the TLR2/6-ligand Pam2CysK4 have repeatedly been shown to induce tolerogenic DC and regulatory T cell responses (Figures 3A,B) (87, 116, 117). In addition, TLR2-ligands have the potential to induce a Th1-promoting cytokine milieu, enhance Ag presentation of endogenous peptides by DCs (117), and suppress IL-5, IL-13, and IFN-γ responses from human house dust mite-allergic patients (Figure 3B) (118).
Here, chemical conjugation of the TLR2-ligand Pam3CysK4 to OVA-derived CD8+ T cell peptide sequences resulted in a rapid and enhanced uptake in DCs (119). Moreover, dual TLR2/7-ligands combining the TLR2-ligand Pam2CysK4 and the synthetic TLR7-ligand CL264 into a single molecule were shown to induce strongly activated mDCs co-producing IL-10 and pro-inflammatory IL-6 (Figure 3A) (88). In vitro, these mDCs suppressed both DNP-induced, IgE- and Ag-dependent mast cell degranulation and IL-5 secretion from OVA-specific DO11.10 CD4+ TC (Figure 3B) (88). In vivo application of one of these ligands, CL531, was found to suppress allergen-specific IgE production in a mouse model of OVA-induced intestinal allergy, suggesting that such TLR2/7-ligands have the potential to induce Th1-biased immune modulation in vivo (88).
Ahrens et al. described LPS stimulation to strongly enhance IL-10 production from mouse BM-DCs (Figure 3A). In co-culture with allergen-specific naive CD4+ T cells, LPS-stimulated BM-DCs suppressed the secretion of Th1 and Th2 cytokines in an IL-10-dependent manner (89). Here, LPS priming of BM-DCs resulted in the differentiation of a Tr1-like T-cell subset upon co-culture of the primed DCs with naive T cells (89). Accordingly, Wakkach et al. reported LPS-, but not CpG-stimulation, to induce enhanced IL-10 secretion from IL-10-treated, CD11clowB220−CD45RB+ DCs (Figure 3A) (90). However, the suppressive capacity of LPS-primed BM-DC in vitro did not translate into suppression of allergic airway disease in vivo since intranasal administration of these LPS + IL-10-primed BM-DCs into mice was unable to prevent allergic airway inflammation in a mouse model of OVA-induced asthma (89). Here, vaccination with theseBM-DCs led to an even stronger eosinophilic airway inflammation and AHR accompanied by significantly increased levels of IL-5 and IL-13 in BAL fluid (89).
While LPS cannot be applied in humans due to its high toxicity and pyrogenicity, monophosphoryl lipid A (MPLA), a detoxified TLR4-ligand derived from Salmonella minnesota, is already applied as adjuvant in several vaccine formulations. Here, vaccines containing MPLA have been licensed or are in phase III trials including Fendrix (hepatitis B), Cervarix (human papillomavirus-16 and human papillomavirus-18), and RTS,S (malaria) (120–122). For the treatment of allergies, MPLA mixed with grass pollen extract was shown to result in enhanced production of IFN-γ and reduce the production of IL-5 in PBMC from grass pollen-allergic patients (123). In line with these results, MPLA was shown to induce mouse BM-DC activation (CD40 upregulation) and secretion of both pro- (IL-1β, IL-6, TNF-α) and anti-inflammatory (IL-10) cytokines in vitro (Figure 3A) (91). Of note, when MPLA-stimulated mouse BM-DCs were co-cultured with DO11.10 CD4+ T cells in vitro MPLA was shown to boost OVA-specific IL-4 and IL-5 secretion while dose-dependently suppressing IFN-γ secretion displaying a discrepancy between the results obtained in vitro and in clinical trials (91).
In addition, synthetic oligodeoxynucleotides containing CpG motifs (TLR9-ligands) either alone (124–126) or chemically linked to allergens (127–129) have been described to promote Th1 cytokine responses and decrease synthesis of IgE antibodies in allergic individuals. While these constructs were shown to induce the production of IL-12 and IL-18 from human moDCs (125) and induce IL-12, IFN-α, IFN-γ, IL-6, and IL-10 secretion from PBMCs (126), up to now no DC-derived IL-10 secretion has been reported upon application of these CpG-based vaccines.
In our own studies, we evaluated the induction of IL-10-producing mDCs using fusion proteins consisting of the recombinant TLR5-ligand flagellin A (rFlaA) from L. monocytogenes and either OVA from hen’s egg as a model allergen (rFlaA:OVA) (92–94), the major mugwort allergen Artemisia vulgaris allergen 1 (Art v1) (rFlaA:Artv1) (95), or the major birch pollen allergen Betula verrucosa allergen 1 (Bet v 1) (rFlaA:Betv1) (96). Such fusion proteins efficiently target TLR5+ immune cells, e.g., DCs, macrophages, and epithelial cells which take up, process, and present the fused antigen in the context of the flagellin-mediated cell activation.
Mouse bone marrow-derived mDCs stimulated with such fusion proteins were strongly activated [upregulation of CD40, CD69, CD80, CD86, programmed cell death 1 ligand 2 (B7-DC, CD273), PD-L1], displayed highly increased levels of the target receptor TLR5 on their cell surface, and secreted both pro- (IL-1β, IL-6) and anti-inflammatory (IL-10) cytokines (Figure 3A) (93, 95, 96). Interestingly, the non-fused mixture of both components (flagellin and allergen) did not have a comparable effect (93, 95, 96).
When co-cultured with allergen-specific CD4+ T cells, these DCs efficiently suppressed both allergen-induced Th1 and Th2 cytokine secretion in vitro (Figure 3B) (93, 95, 96). In this experimental setting, the flagellin fusion protein-mediated suppression of both Th1- and Th2-cytokine secretion was dependent on IL-10, since this effect was reversed when using either IL-10-neutralizing antibodies or IL-10-deficient mDC as APCs for the co-cultures (93).
In vivo vaccination with the rFlaA:OVA fusion protein efficiently protected mice from OVA-induced gastrointestinal allergy. Here, vaccination with rFlaA:OVA either intraperitoneal (93) or intranasal (92) was sufficient to prevent intestinal allergy induced by continuous challenge with OVA-containing food pellets. Interestingly, core body temperature, body weight, food up-take, and symptom scores were significantly improved in rFlaA:OVA-treated mice in comparison to the respective control groups (92, 93). This protective effect was associated with a reduction of Th2 cytokines in intestinal homogenates, suppression of systemic T cell immune responses, suppression of OVA-specific IgE-, and induction of OVA-specific IgG2a-responses (93). Vaccination with rFlaA and OVA alone or provided as a mixture did neither prevent allergic sensitization nor improve allergy symptom scores.
Mechanistically, stimulation of mDCs with flagellin fusion proteins was shown to result in a stronger uptake into mDCs (92, 96) accompanied by an increased resistance to microsomal digestion (92, 96). Interestingly, stimulation with rFlaA:Betv1 was shown to result in an increased metabolic activity of the stimulated mDCs characterized by a high rate of glycolysis followed by lactic acid fermentation, known as the Warburg effect (130). Further analysis suggested an activation of the mammalian target of rapamycin 1 complex in mDCs stimulated with the fusion protein (96). In this context, recent studies suggest that mTOR is not only a master regulator of cell metabolic function but also regulates innate immune responses (131).
Inhibition of the mTOR complex by pre-treatment of the cells with rapamycin dose-dependently suppressed the induction of anti-inflammatory IL-10 secretion by rFlaA:Betv1, but not pro-inflammatory cytokine secretion (IL-1β and IL-6). These findings show that interestingly, the immune-modulatory cytokine secretion, and therefore the DC tolerizing capacity, of this vaccine candidate was linked to the activation of mDC metabolism. Similar results were obtained for a fusion construct consisting of FlaA and the major mugwort allergen Art v 1 (95).
Non-TLR Ligands with DC-Tolerizing Potential
In the context of allergy treatment, some non-TLR ligands have been reported to induce DCs with tolerizing potential. Here, Bordetella pertussis-derived filamentous hemagglutinin was shown to induce the production of IL-10 by DCs promoting the differentiation of CD4+CD25+CCR5highCD28lowCTLA-4low IL-10- and IL-5-co-producing Tr1 cells (97).
Moreover, Gerstmayr and colleagues generated a recombinant fusion protein of a bacterial surface (S-layer) protein of Geobacillus stearothermophilus (SbsC) and the major birch pollen allergen Bet v 1 as a vaccine candidate to improve the treatment of birch pollen allergy. The SbsC:Bet v 1 fusion protein displayed reduced mediator-releasing capacity, while both preserving Bet v 1-T cell epitopes, and the potency to induce IFN-γ and IL-10 production in Bet v 1-specific Th2-biased T cell clones (98). DCs stimulated with the fusion protein were shown to have a semi-mature phenotype characterized by enhanced expression of CD40, CD80, and CD86 which were still lower than the levels induced by stimulation with LPS (Figure 3A) (98). Moreover, the SbsC:Bet v 1 fusion protein strongly increased DC-derived IL-10 and IL-12 secretion (98). Functionally, DCs matured with SbsC:Bet v 1 induced the IL-12- and IFN-γ-dependent differentiation of naive T cells into IFN-γ-producing T cells co-producing IL-4, suggesting a Th0 phenotype (Figure 3B) (98). In addition, naive T cells also differentiated into IL-10-producing CD4+CD25+Foxp3+CLTA-4+ regulatory T cells capable of active suppression, thus promoting the simultaneous differentiation of Th0/Th1 cells and regulatory T cells (Figure 3B) (98).
Other Factors Contributing to the Inhibitory Capacity of DCs
Although many studies described the suppressive capacity of IL-10 secreting DCs, several other factors were reported to also mediate suppression of T cell responses without inducing DC-derived IL-10 production. Here, LPS- or polyriboinosinic-polyribocytidilic acid-induced production of IL-1β, indoleamine 2,3-dioxygenase (132), transforming growth factor-beta 1, vitamin D3 (133), corticosteroids, cyclosporine (134), as well as neuropeptides have been used to generate tolerogenic DCs (135, 136). Therefore, although the induction of IL-10 reproducibly leads to a tolerogenic phenotype of the induced DCs, other agents may also be used to generate DCs able to suppress T cells responses.
Summary
Because of their potent T cell stimulatory as well as regulatory properties DCs have become a highly attractive tool in vaccine development to modulate antigen-specific immune responses.
While for cancer treatment and vaccination against infectious diseases the efficient induction of adaptive immune responses against the target antigens is the desired outcome when applying DC-based vaccination approaches, the therapy of autoimmune diseases, transplantat rejection, allergic reactions, or the control of chronic inflammation aims to induce DCs with tolerogenic properties.
Comparing IL-10-producing DCs induced either by IL-10-treatment or IL-10-transduction to IL-10-secreting DCs generated by stimulation with bacteria and bacterial components has revealed striking differences in the phenotype of the induced DCs and therefore the mechanism of tolerization: tolerogenic DCs generated by non-bacterial stimuli are arrested in an immature or semi-mature state, characterized by the production of reduced amounts of cytokines that promote T cell activation such as IL-12 and IL-6, a reduced capacity to present exogenous antigens, and the expression of lower amounts of co-stimulatory molecules.
In contrast to this, treatment of DCs with live or killed bacteria as well as isolated bacterial components results in the induction of both anti-inflammatory IL-10- as well as pro-inflammatory, Th1-promoting, IL-12 secretion often paralleled by an enhanced expression of co-stimulatory molecules on the stimulated DCs. This induction of Th1-priming, tolerogenic DCs generated by strongly activating stimuli was shown to suppress allergen-specific Th2-responses via the induction of either Th1-like Tregs, CD4+CD25+Foxp3+ Tregs, or Tr1 cells.
Therefore, while displaying the ability to directly suppress both Th1- and Th2-responses by different mechanisms, IL-10-producing DCs can efficiently modulate antigen-specific-specific immune responses via the induction of T cell subsets with regulatory functions. This makes IL-10-producing DCs promising therapeutics to improve the treatment of allergic diseases.
Over the past few years, we have started to understand the complex molecular mechanisms underlying the immune modulatory capacity of IL-10-producing DCs, identifying novel DC-derived factors that drive T cell tolerization such as DC-derived exosomes, CCL18, and inhibitory molecules like CTLA-4, OX40, Ig-like transcript-22/CD85, or PD-1.
Future studies will undoubtedly further increase our knowledge about the underlying immunological mechanisms allowing us to both refine and improve the application of DC-based vaccination approaches.
Author Contributions
SS performed all research, prepared all the figures and tables, and wrote the manuscript.
Conflict of Interest Statement
The author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Funding
This work has been funded by the budget of the Paul-Ehrlich-Institut, Langen, Germany.
Abbreviations
AIT, allergen-specific immunotherapy; AHR, airway hyperreactivity; APC, antigen-presenting cell; AR, allergic rhinitis; Art v1, major mugwort allergen Artemisia vulgaris allergen 1; B7-DC, programmed cell death 1 ligand 2 (CD273); BAL, bronchoalveolar lavage; BATF3, basic leucine zipper ATF-like 3; BCG, Bacillus Calmette–Guérin; Bet v 1, major birch pollen Betula verrucosa allergen 1; BM-DCs, bone marrow-derived DCs; cAMP, cyclic adenosine monophosphate; CCL-3/18, CC-chemokine ligand 3/18; CCR5/7, CC chemokine receptor 5/7; CD, cluster of differentiation; cDC, conventional DC; CpG-ODN, oligodeoxynucleotides containing CpG motifs; CTLA-4, cytotoxic T lymphocyte-associated antigen 4 (CD152); DC, dendritic cell; Der p 1, major house dust mite allergen Dermatophagoides pteronyssinus peptidase 1; DTH, delayed-type hypersensitivity; FcεRI, high affinity IgE receptor Fc epsilon receptor I; Foxp3, forkhead box protein 3; HLA-DR, human leukocyte antigen DR; ICAM-1, intercellular adhesion molecule 1; ICOS, inducible co-stimulator; ICOS-L, inducible co-stimulator ligand; IDO, indoleamine 2,3-dioxygenase; IFN-α1, interferon alpha 1; IFN-γ, interferon gamma; Ig E/G, immunoglobulin E/G; IL, interleukin; IL-1RN, interleukin 1 receptor antagonist; IL-10R, interleukin 10 receptor; iTregs, inducible Tregs; Jak1, Janus kinase 1; LAG-3, lymphocyte-activation gene 3; LZT, low zone tolerance; MAP kinase, mitogen-activated protein kinase; mDC, myeloid DCs; MHC I/II, major histocompatibility complex I/II; MoDC, monocyte-derived DCs; MPLA, monophosphoryl lipid A; mTOR1, mammalian target of rapamycin 1; MyD88, myeloid differentiation primary response 88; OIT, oral immunotherapy; OX40, TNF receptor superfamily member 4 (CD134); OVA, ovalbumin; PAMP, pathogen-associated molecular pattern; PBMC, peripheral blood mononuclear cell; pDC, plasmacytoid DC; PD-1/2, programmed death-1/2; PD-L1/2, programmed death ligand ½; PDE4, phosphodiesterase-4; PRR, pathogen-recognition receptor; rFlaA, recombinant TLR5-ligand flagellin A; rFlaA:Artv1, recombinant fusion protein consisting of flagellin A and Art v 1; rFlaA:Betv1, recombinant fusion protein consisting of flagellin A and Bet v 1; rFlaA:OVA, recombinant fusion protein consisting of flagellin A and OVA; SbsC, surface (S-layer) protein of Geobacillus stearothermophilus; SbsC:Bet v 1, recombinant fusion protein consisting of SbsC and Bet v 1; SLIT, sublingual immunotherapy; SOCS-3, suppressor of cytokine signaling 3; STAT 1/5/6, signal transducer and activator of transcription 1/5/6; T-bet, T-box transcription factor TBX21; TGF-β, transforming growth factor beta; Th0/1/2/17, T-helper 0/1/2/17 cell; TLR, toll-like receptor; TRIF, TIR-domain-containing adapter-inducing interferon-β; TNF-α, tumor necrosis factor alpha; Tr1, regulatory T cell type I; Treg, regualory T cell; Tyk2, tyrosine kinase 2.
References
1. Koya T, Matsuda H, Takeda K, Matsubara S, Miyahara N, Balhorn A, et al. IL-10-treated dendritic cells decrease airway hyperresponsiveness and airway inflammation in mice. J Allergy ClinImmunol (2007) 119:1241–50. doi:10.1016/j.jaci.2007.01.039
2. Gentile DA, Schreiber R, Howe-Adams J, Trecki J, Patel A, Angelini B, et al. Diminished dendritic cell interleukin 10 production in atopic children. Ann Allergy Asthma Immunol (2004) 92:538–44. doi:10.1016/S1081-1206(10)61761-9
3. Besche V, Wiechmann N, Castor T, Trojandt S, Höhn Y, Kunkel H, et al. Dendritic cells lentivirally engineered to overexpress interleukin-10 inhibit contact hypersensitivity responses, despite their partial activation induced by transduction-associated physical stress. J Gene Med (2010) 12(3):231–43. doi:10.1002/jgm.1436
4. Henry E, Desmet CJ, Garzé V, Fiévez L, Bedoret D, Heirman C, et al. Dendritic cells genetically engineered to express IL-10 induce long-lasting antigen-specific tolerance in experimental asthma. J Immunol (2008) 181:7230–42. doi:10.4049/jimmunol.181.10.7230
5. Steinman RM. The dendritic cell system and its role in immunogenicity. Annu Rev Immunol (1991) 9:271–96. doi:10.1146/annurev.iy.09.040191.001415
6. Schuurhuis DH, Fu N, Ossendorp F, Melief CJM. Ins and outs of dendritic cells. Int Arch Allergy Immunol (2006) 140:53–72. doi:10.1159/000092002
7. Lanzavecchia A. Mechanisms of antigen uptake for presentation. Curr Opin Immunol (1996) 8:348–54. doi:10.1016/S0952-7915(96)80124-5
8. Banchereau J, Briere F, Caux C, Davoust J, Lebecque S, Liu YJ, et al. Immunobiology of dendritic cells. Annu Rev Immunol (2000) 18:767–811. doi:10.1146/annurev.immunol.18.1.767
9. Chan VW, Kothakota S, Rohan MC, Panganiban-Lustan L, Gardner JP, Wachowicz MS, et al. Secondary lymphoid-tissue chemokine (SLC) is chemotactic for mature dendritic cells. Blood (1999) 93:3610–6.
10. Sallusto F, Schaerli P, Loetscher P, Schaniel C, Lenig D, Mackay CR, et al. Rapid and coordinated switch in chemokine receptor expression during dendritic cell maturation. Eur J Immunol (1998) 28:2760–9. doi:10.1002/(SICI)1521-4141(199809)28:09<2760::AID-IMMU2760>3.0.CO;2-N
11. Xu X, Guo Z, Jiang X, Yao Y, Gao Q, Ding Y, et al. Regulatory dendritic cells program generation of interleukin-4-producing alternative memory CD4 T cells with suppressive activity. Blood (2011) 117:1218–27. doi:10.1182/blood-2010-05-285494
12. Guida G, Boita M, Scirelli T, Bommarito L, Heffler E, Badiu I, et al. Innate and lymphocytic response of birch-allergic patients before and after sublingual immunotherapy. Allergy Asthma Proc (2012) 33:411–5. doi:10.2500/aap.2012.33.3588
13. Steinman RM, Banchereau J. Taking dendritic cells into medicine. Nature (2007) 449:419–26. doi:10.1038/nature06175
14. Steinman RM, Hawiger D, Nussenzweig MC. Tolerogenic dendritic cells. Annu Rev Immunol (2003) 21:685–711. doi:10.1146/annurev.immunol.21.120601.141040
15. Li MO, Flavell RA. Contextual regulation of inflammation: a duet by transforming growth factor-beta and interleukin-10. Immunity (2008) 28:468–76. doi:10.1016/j.immuni.2008.03.003
16. Escobar A, Aguirre A, Guzmán MA, González R, Catalán D, Acuña-Castillo C, et al. Tolerogenic dendritic cells derived from donors with natural rubber latex allergy modulate allergen-specific T-cell responses and IgE production. PLoS One (2014) 9:e85930. doi:10.1371/journal.pone.0085930
17. Francis JN, Till SJ, Durham SR. Induction of IL-10+CD4+CD25+ T cells by grass pollen immunotherapy. J Allergy Clin Immunol (2003) 111:1255–61. doi:10.1067/mai.2003.1570
18. Mahnke K, Ring S, Bedke T, Karakhanova S, Enk AH. Interaction of regulatory T cells with antigen-presenting cells in health and disease. Chem Immunol Allergy (2008) 94:29–39. doi:10.1159/000154854
19. Pulendran B, Ahmed R. Translating innate immunity into immunological memory: implications for vaccine development. Cell (2006) 124:849–63. doi:10.1016/j.cell.2006.02.019
20. Moser M, Murphy KM. Dendritic cell regulation of TH1-TH2 development. Nat Immunol (2000) 1:199–205. doi:10.1038/79734
21. Wu K, Bi Y, Sun K, Xia J, Wang Y, Wang C. Suppression of allergic inflammation by allergen-DNA-modified dendritic cells depends on the induction of Foxp3+ regulatory T cells. Scand J Immunol (2008) 67:140–51. doi:10.1111/j.1365-3083.2007.02050.x
22. Couper KN, Blount DG, Riley EM. IL-10: the master regulator of immunity to infection. J Immunol (2008) 180:5771–7. doi:10.4049/jimmunol.180.9.5771
23. Moore KW, de Waal Malefyt R, Coffman RL, O’Garra A. Interleukin-10 and the interleukin-10 receptor. Annu Rev Immunol (2001) 19:683–765. doi:10.1146/annurev.immunol.19.1.683
24. Saraiva M, O’Garra A. The regulation of IL-10 production by immune cells. Nat Rev Immunol (2010) 10:170–81. doi:10.1038/nri2711
25. Clausen BE, Girard-Madoux MJH. IL-10 control of dendritic cells in the skin. Oncoimmunology (2014) 2:e23186–23184. doi:10.4161/onci.23186
26. Vieira P, de Waal Malefyt R, Dang MN, Johnson KE, Kastelein R, Fiorentino DF, et al. Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading frame BCRFI. Proc Natl Acad Sci U S A (1991) 88:1172–6. doi:10.1073/pnas.88.4.1172
27. Han X, Wang S, Fan Y, Yang J, Jiao L, Qiu H, et al. Chlamydia infection induces ICOS ligand-expressing and IL-10-producing dendritic cells that can inhibit airway inflammation and mucus overproduction elicited by allergen challenge in BALB/c mice. J Immunol (2006) 176:5232–9. doi:10.4049/jimmunol.176.9.5232
28. Levings MK, Gregori S, Tresoldi E, Cazzaniga S, Bonini C, Roncarolo MG. Differentiation of Tr1 cells by immature dendritic cells requires IL-10 but not CD25+CD4+ Tr cells. Blood (2005) 105:1162–9. doi:10.1182/blood-2004-03-1211
29. Groux H, Bigler M, de Vries JE, Roncarolo MG. Interleukin-10 induces a long-term antigen-specific anergic state in human CD4+ T cells. J Exp Med (1996) 184:19–29. doi:10.1084/jem.184.1.19
30. Pacciani V, Gregori S, Chini L, Corrente S, Chianca M, Moschese V, et al. Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites. J Allergy Clin Immunol (2010) 125:727–36. doi:10.1016/j.jaci.2009.12.004
31. Oh J-W, Seroogy CM, Meyer EH, Akbari O, Berry G, Fathman CG, et al. CD4 T-helper cells engineered to produce IL-10 prevent allergen-induced airway hyperreactivity and inflammation. J Allergy ClinImmunol (2002) 110:460–8. doi:10.1067/mai.2002.127512
32. Akbari O, DeKruyff RH, Umetsu DT. Pulmonary dendritic cells producing IL-10 mediate tolerance induced by respiratory exposure to antigen. Nat Immunol (2001) 2:725–31. doi:10.1038/90667
33. Kearley J, Barker JE, Robinson DS, Lloyd CM. Resolution of airway inflammation and hyperreactivity after in vivo transfer of CD4+CD25+ regulatory T cells is interleukin 10 dependent. J Exp Med (2005) 202:1539–47. doi:10.1084/jem.20051166
34. Berg DJ, Leach MW, Kühn R, Rajewsky K, Müller W, Davidson NJ, et al. Interleukin 10 but not interleukin 4 is a natural suppressant of cutaneous inflammatory responses. J Exp Med (1995) 182:99–108. doi:10.1084/jem.182.1.99
35. Pestka S, Krause CD, Sarkar D, Walter MR, Shi Y, Fisher PB. Interleukin-10 and related cytokines and receptors. Annu Rev Immunol (2004) 22:929–79. doi:10.1146/annurev.immunol.22.012703.104622
36. de Waal Malefyt R, Haanen J, Spits H, Roncarolo MG, Velde te A, Figdor C, et al. Interleukin 10 (IL-10) and viral IL-10 strongly reduce antigen-specific human T cell proliferation by diminishing the antigen-presenting capacity of monocytes via downregulation of class II major histocompatibility complex expression. J Exp Med (1991) 174:915–24. doi:10.1084/jem.174.4.915
37. Kotenko SV, Krause CD, Izotova LS, Pollack BP, Wu W, Pestka S. Identification and functional characterization of a second chain of the interleukin-10 receptor complex. EMBO J (1997) 16:5894–903. doi:10.1093/emboj/16.19.5894
38. Finbloom DS, Winestock KD. IL-10 induces the tyrosine phosphorylation of tyk2 and Jak1 and the differential assembly of STAT1 alpha and STAT3 complexes in human T cells and monocytes. J Immunol (1995) 155:1079–90.
39. Weber-Nordt RM, Riley JK, Greenlund AC, Moore KW, Darnell JE, Schreiber RD. Stat3 recruitment by two distinct ligand-induced, tyrosine-phosphorylated docking sites in the interleukin-10 receptor intracellular domain. J Biol Chem (1996) 271:27954–61. doi:10.1074/jbc.271.44.27954
40. Berti FCB, Pereira APL, Cebinelli GCM, Trugilo KP, Brajão de Oliveira K. The role of interleukin 10 in human papilloma virus infection and progression to cervical carcinoma. Cytokine Growth Factor Rev (2017) 34:1–13. doi:10.1016/j.cytogfr.2017.03.002
41. Berlato C, Cassatella MA, Kinjyo I, Gatto L, Yoshimura A, Bazzoni F. Involvement of suppressor of cytokine signaling-3 as a mediator of the inhibitory effects of IL-10 on lipopolysaccharide-induced macrophage activation. J Immunol (2002) 168:6404–11. doi:10.4049/jimmunol.168.12.6404
42. Williams L, Bradley L, Smith A, Foxwell B. Signal transducer and activator of transcription 3 is the dominant mediator of the anti-inflammatory effects of IL-10 in human macrophages. J Immunol (2004) 172:567–76. doi:10.4049/jimmunol.172.1.567
43. Dinarello CA. The interleukin-1 family: 10 years of discovery. FASEB J (1994) 8(15):1314–25. doi:10.1096/fasebj.8.15.8001745
44. Biswas PS, Pedicord V, Ploss A, Menet E, Leiner I, Pamer EG. Pathogen-specific CD8 T cell responses are directly inhibited by IL-10. J Immunol (2007) 179:4520–8. doi:10.4049/jimmunol.179.7.4520
45. Dickensheets HL, Donnelly RP. IFN-gamma and IL-10 inhibit induction of IL-1 receptor type I and type II gene expression by IL-4 and IL-13 in human monocytes. J Immunol (1997) 159:6226–33.
46. Le T, Tversky J, Chichester KL, Bieneman AP, Huang S-K, Wood RA, et al. Interferons modulate Fc epsilon RI-dependent production of autoregulatory IL-10 by circulating human monocytoid dendritic cells. J Allergy Clin Immunol (2009) 123:217–23. doi:10.1016/j.jaci.2008.09.013
47. Frischmeyer-Guerrerio PA, Keet CA, Guerrerio AL, Chichester KL, Bieneman AP, Hamilton RG, et al. Modulation of dendritic cell innate and adaptive immune functions by oral and sublingual immunotherapy. Clin Immunol (2014) 155:47–59. doi:10.1016/j.clim.2014.08.006
48. Bellinghausen I, Knop J, Saloga J. The role of interleukin 10 in the regulation of allergic immune responses. Int Arch Allergy Immunol (2001) 126:97–101. doi:10.1159/000049499
49. Akbari O, Umetsu DT. Role of regulatory dendritic cells in allergy and asthma. Curr Opin Allergy Clin Immunol (2004) 4:533–8. doi:10.1097/00130832-200412000-00010
50. Braza F, Chesne J, Castagnet S, Magnan A, Brouard S. Regulatory functions of B cells in allergic diseases. Allergy (2014) 69:1454–63. doi:10.1111/all.12490
51. Faith A, Singh N, Chevretton E, Roberts D, Lee T, Corrigan C, et al. Counter regulation of the high affinity IgE receptor, FcepsilonRI, on human airway dendritic cells by IL-4 and IL-10. Allergy (2009) 64:1602–7. doi:10.1111/j.1398-9995.2009.02060.x
52. Akbari O, Freeman GJ, Meyer EH, Greenfield EA, Chang TT, Sharpe AH, et al. Antigen-specific regulatory T cells develop via the ICOS-ICOS-ligand pathway and inhibit allergen-induced airway hyperreactivity. Nat Med (2002) 8:1024–32. doi:10.1038/nm745
53. Enk AH, Angeloni VL, Udey MC, Katz SI. Inhibition of langerhans cell antigen-presenting function by IL-10. A role for IL-10 in induction of tolerance. J Immunol (1993) 151:2390–8.
54. Punnonen J, de Waal Malefyt R, van Vlasselaer P, Gauchat JF, de Vries JE. IL-10 and viral IL-10 prevent IL-4-induced IgE synthesis by inhibiting the accessory cell function of monocytes. J Immunol (1993) 151:1280–9.
55. Francis JN, James LK, Paraskevopoulos G, Wong C, Calderon MA, Durham SR, et al. Grass pollen immunotherapy: IL-10 induction and suppression of late responses precedes IgG4 inhibitory antibody activity. J Allergy Clin Immunol (2008) 121:1120–5.e2. doi:10.1016/j.jaci.2008.01.072
56. Bohle B, Kinaciyan T, Gerstmayr M, Radakovics A, Jahn-Schmid B, Ebner C. Sublingual immunotherapy induces IL-10-producing T regulatory cells, allergen-specific T-cell tolerance, and immune deviation. J Allergy ClinImmunol (2007) 120:707–13. doi:10.1016/j.jaci.2007.06.013
57. Súkeníková L, Černý V, Novotná O, Petrásková P, Boráková K, Kolářová L, et al. Different capacity of in vitro generated myeloid dendritic cells of newborns of healthy and allergic mothers to respond to probiotic strain E. coli O83:K24:H31. Immunol Lett (2017) 189:82–9. doi:10.1016/j.imlet.2017.05.013
58. Niwa Y. Elevated RANTES levels in plasma or skin and decreased plasma IL-10 levels in subsets of patients with severe atopic dermatitis. Arch Dermatol (2000) 136:125–6. doi:10.1001/archderm.136.1.125
59. Stelmach I, Jerzynska J, Kuna P. A randomized, double-blind trial of the effect of glucocorticoid, antileukotriene and beta-agonist treatment on IL-10 serum levels in children with asthma. Clin Exp Allergy (2002) 32:264–9. doi:10.1046/j.1365-2222.2002.01286.x
60. Gentile DA, Patel A, Ollila C, Fireman P, Zeevi A, Doyle WJ, et al. Diminished IL-10 production in subjects with allergy after infection with influenza A virus. J Allergy ClinImmunol (1999) 103:1045–8. doi:10.1016/S0091-6749(99)70177-6
61. Tournoy KG, Kips JC, Pauwels RA. Endogenous interleukin-10 suppresses allergen-induced airway inflammation and nonspecific airway responsiveness. Clin Exp Allergy (2000) 30:775–83. doi:10.1046/j.1365-2222.2000.00838.x
62. Anderson AE, Mackerness KJ, Aizen M, Carr VA, Nguyen D, Pre Du F, et al. Seasonal changes in suppressive capacity of CD4+ CD25+ T cells from patients with hayfever are allergen-specific and may result in part from expansion of effector T cells among the CD25+ population. Clin Exp Allergy (2009) 39:1693–9. doi:10.1111/j.1365-2222.2009.03320.x
63. Wei P, Hu G-H, Kang H-Y, Yao H-B, Kou W, Liu H, et al. An aryl hydrocarbon receptor ligand acts on dendritic cells and T cells to suppress the Th17 response in allergic rhinitis patients. Lab Invest (2014) 94:528–35. doi:10.1038/labinvest.2014.8
64. Pilette C, Jacobson MR, Ratajczak C, Detry B, Banfield G, VanSnick J, et al. Aberrant dendritic cell function conditions Th2-cell polarization in allergic rhinitis. Allergy (2013) 68:312–21. doi:10.1111/all.12090
65. Lied GA, Vogelsang P, Berstad A, Appel S. Dendritic cell populations in patients with self-reported food hypersensitivity. Int J Gen Med (2011) 4:389–96. doi:10.2147/IJGM.S17655
66. Frischmeyer-Guerrerio PA, Guerrerio AL, Chichester KL, Bieneman AP, Hamilton RA, Wood RA, et al. Dendritic cell and T cell responses in children with food allergy. Clin Exp Allergy (2010) 41:61–71. doi:10.1111/j.1365-2222.2010.03606.x
67. Akdis CA, Akdis M. Mechanisms of immune tolerance to allergens: role of IL-10 and Tregs. J Clin Invest (2014) 124:4678–80. doi:10.1172/JCI78891
68. Nasser SM, Ying S, Meng Q, Kay AB, Ewan PW. Interleukin-10 levels increase in cutaneous biopsies of patients undergoing wasp venom immunotherapy. Eur J Immunol (2001) 31:3704–13. doi:10.1002/1521-4141(200112)31:12<3704::AID-IMMU3704>3.0.CO;2-3
69. Nouri-Aria KT, Wachholz PA, Francis JN, Jacobson MR, Walker SM, Wilcock LK, et al. Grass pollen immunotherapy induces mucosal and peripheral IL-10 responses and blocking IgG activity. J Immunol (2004) 172:3252–9. doi:10.4049/jimmunol.172.5.3252
70. Akdis CA, Blesken T, Akdis M, Wüthrich B, Blaser K. Role of interleukin 10 in specific immunotherapy. J Clin Invest (1998) 102:98–106. doi:10.1172/JCI2250
71. Jutel M, Akdis M, Budak F, Aebischer-Casaulta C, Wrzyszcz M, Blaser K, et al. IL-10 and TGF-beta cooperate in the regulatory T cell response to mucosal allergens in normal immunity and specific immunotherapy. Eur J Immunol (2003) 33:1205–14. doi:10.1002/eji.200322919
72. Möbs C, Slotosch C, Löffler H, Jakob T, Hertl M, Pfützner W. Birch pollen immunotherapy leads to differential induction of regulatory T cells and delayed helper T cell immune deviation. J Immunol (2010) 184:2194–203. doi:10.4049/jimmunol.0901379
73. van de Veen W, Stanic B, Yaman G, Wawrzyniak M, Söllner S, Akdis DG, et al. IgG4 production is confined to human IL-10-producing regulatory B cells that suppress antigen-specific immune responses. J Allergy Clin Immunol (2013) 131:1204–12. doi:10.1016/j.jaci.2013.01.014
74. Kunz S, Dolch A, Surianarayanan S, Dorn B, Bewersdorff M, Alessandrini F, et al. T cell derived IL-10 is dispensable for tolerance induction in a murine model of allergic airway inflammation. Eur J Immunol (2016) 46:2018–27. doi:10.1002/eji.201646319
75. Bros M, Montermann E, Cholaszczyńska A, Reske-Kunz AB. The phosphodiesterase 4 inhibitor roflumilast augments the Th17-promoting capability of dendritic cells by enhancing IL-23 production, and impairs their T cell stimulatory activity due to elevated IL-10. Int Immunopharmacol (2016) 35:174–84. doi:10.1016/j.intimp.2016.03.025
76. Kambayashi T, Wallin RP, Ljunggren HG. cAMP-elevating agents suppress dendritic cell function. J Leukoc Biol (2001) 70:903–10. doi:10.1189/jlb.70.6.903
77. Li X, Yang A, Huang H, Zhang X, Town J, Davis B, et al. Induction of type 2 T helper cell allergen tolerance by IL-10-differentiated regulatory dendritic cells. Am J Respir Cell Mol Biol (2010) 42:190–9. doi:10.1165/rcmb.2009-0023OC
78. Bellinghausen I, König B, Böttcher I, Knop J, Saloga J. Inhibition of human allergic T-helper type 2 immune responses by induced regulatory T cells requires the combination of interleukin-10-treated dendritic cells and transforming growth factor-beta for their induction. Clin Exp Allergy (2006) 36:1546–55. doi:10.1111/j.1365-2222.2006.02601.x
79. Bellinghausen I, Reuter S, Martin H, Maxeiner J, Luxemburger U, Türeci Ö, et al. Enhanced production of CCL18 by tolerogenic dendritic cells is associated with inhibition of allergic airway reactivity. J Allergy Clin Immunol (2012) 130:1384–93. doi:10.1016/j.jaci.2012.08.039
80. Nakagome K, Dohi M, Okunishi K, Komagata Y, Nagatani K, Tanaka R, et al. In vivo IL-10 gene delivery suppresses airway eosinophilia and hyperreactivity by down-regulating APC functions and migration without impairing the antigen-specific systemic immune response in a mouse model of allergic airway inflammation. J Immunol (2005) 174:6955–66. doi:10.4049/jimmunol.174.11.6955
81. Kim SH, Lechman ER, Bianco N, Menon R, Keravala A, Nash J, et al. Exosomes derived from IL-10-treated dendritic cells can suppress inflammation and collagen-induced arthritis. J Immunol (2005) 174:6440–8. doi:10.4049/jimmunol.174.10.6440
82. Ruffner MA, Kim SH, Bianco NR, Francisco LM, Sharpe AH, Robbins PD. B7-1/2, but not PD-L1/2 molecules, are required on IL-10-treated tolerogenic DC and DC-derived exosomes for in vivo function. Eur J Immunol (2009) 39:3084–90. doi:10.1002/eji.200939407
83. Engler DB, Reuter S, van Wijck Y, Urban S, Kyburz A, Maxeiner J, et al. Effective treatment of allergic airway inflammation with Helicobacter pylori immunomodulators requires BATF3-dependent dendritic cells and IL-10. Proc Natl Acad Sci U S A (2014) 111:11810–5. doi:10.1073/pnas.1410579111
84. Bilenki L, Gao X, Wang S, Yang J, Fan Y, Han X, et al. Dendritic cells from mycobacteria-infected mice inhibits established allergic airway inflammatory responses to ragweed via IL-10- and IL-12-secreting mechanisms. J Immunol (2010) 184:7288–96. doi:10.4049/jimmunol.0902829
85. Stock P, Akbari O, Berry G, Freeman GJ, DeKruyff RH, Umetsu DT. Induction of T helper type 1-like regulatory cells that express Foxp3 and protect against airway hyper-reactivity. Nat Immunol (2004) 5:1149–56. doi:10.1038/ni1122
86. Pochard P, Vickery B, Berin MC, Grishin A, Sampson HA, Caplan M, et al. Targeting toll-like receptors on dendritic cells modifies the T(H)2 response to peanut allergens in vitro. J Allergy Clin Immunol (2010) 126:92–7.e5. doi:10.1016/j.jaci.2010.04.003
87. Tsai Y-G, Yang KD, Niu D-M, Chien J-W, Lin C-Y. TLR2 agonists enhance CD8+Foxp3+ regulatory T cells and suppress Th2 immune responses during allergen immunotherapy. J Immunol (2010) 184:7229–37. doi:10.4049/jimmunol.1000083
88. Laiño J, Wangorsch A, Blanco F, Wolfheimer S, Krause M, Flaczyk A, et al. Targeting of immune cells by Dual TLR2/7 ligands suppresses features of allergic Th2 immune responses in mice. J Immunol Res (2017) 2017:7983217. doi:10.1155/2017/7983217
89. Ahrens B, Freund T, Rha R-D, Dittrich A-M, Quarcoo D, Hutloff A, et al. Lipopolysaccharide stimulation of dendritic cells induces interleukin-10 producing allergen-specific T cells in vitro but fails to prevent allergic airway disease. Exp Lung Res (2009) 35:307–23. doi:10.1080/01902140802709460
90. Wakkach A, Fournier N, Brun V, Breittmayer J-P, Cottrez F, Groux H. Characterization of dendritic cells that induce tolerance and T regulatory 1 cell differentiation in vivo. Immunity (2003) 18:605–17. doi:10.1016/S1074-7613(03)00113-4
91. Schülke S, Flaczyk A, Vogel L, Gaudenzio N, Angers I, Löschner B, et al. MPLA shows attenuated pro-inflammatory properties and diminished capacity to activate mast cells in comparison with LPS. Allergy (2015) 70(10):1259–68. doi:10.1111/all.12675
92. Schülke S, Wolfheimer S, Gadermaier G, Wangorsch A, Siebeneicher S, Briza P, et al. Prevention of intestinal allergy in mice by rflaA:Ova is associated with enforced antigen processing and TLR5-dependent IL-10 secretion by mDC. PLoS One (2014) 9:e87822. doi:10.1371/journal.pone.0087822
93. Schülke S, Burggraf M, Waibler Z, Wangorsch A, Wolfheimer S, Kalinke U, et al. A fusion protein of flagellin and ovalbumin suppresses the TH2 response and prevents murine intestinal allergy. J Allergy Clin Immunol (2011) 128:1340–8.e12. doi:10.1016/j.jaci.2011.07.036
94. Schülke S, Waibler Z, Mende M-S, Zoccatelli G, Vieths S, Toda M, et al. Fusion protein of TLR5-ligand and allergen potentiates activation and IL-10 secretion in murine myeloid DC. Mol Immunol (2010) 48:341–50. doi:10.1016/j.molimm.2010.07.006
95. Schülke S, Kuttich K, Wolfheimer S, Duschek N, Wangorsch A, Reuter A, et al. Conjugation of wildtype and hypoallergenic mugwort allergen Art v 1 to flagellin induces IL-10-DC and suppresses allergen-specific TH2-responses in vivo. Sci Rep (2017) 7:11782. doi:10.1038/s41598-017-11972-w
96. Schülke S, Fiedler A-H, Ann-Christine J, Flaczyk A, Wolfheimer S, Anke H, et al. Critical role of mammalian target of rapamycin for IL-10 DC induction by a flagellin FlaA-conjugate preventing allergic sensitization. J Allergy ClinImmunol (2017). doi:10.1016/j.jaci.2017.07.002
97. McGuirk P, McCann C, Mills KHG. Pathogen-specific T regulatory 1 cells induced in the respiratory tract by a bacterial molecule that stimulates interleukin 10 production by dendritic cells: a novel strategy for evasion of protective T helper type 1 responses by Bordetella pertussis. J Exp Med (2002) 195:221–31. doi:10.1084/jem.20011288
98. Gerstmayr M, Ilk N, Schabussova I, Jahn-Schmid B, Egelseer EM, Sleytr UB, et al. A novel approach to specific allergy treatment: the recombinant allergen-S-layer fusion protein rSbsC-Bet v 1 matures dendritic cells that prime Th0/Th1 and IL-10-producing regulatory T cells. J Immunol (2007) 179:7270–5. doi:10.4049/jimmunol.179.11.7270
99. Steinbrink K, Jonuleit H, Müller G, Schuler G, Knop J, Enk AH. Interleukin-10-treated human dendritic cells induce a melanoma-antigen-specific anergy in CD8(+) T cells resulting in a failure to lyse tumor cells. Blood (1999) 93:1634–42.
100. Steinbrink K, Wölfl M, Jonuleit H, Knop J, Enk AH. Induction of tolerance by IL-10-treated dendritic cells. J Immunol (1997) 159:4772–80.
101. Bellinghausen I, Brand U, Steinbrink K, Enk AH, Knop J, Saloga J. Inhibition of human allergic T-cell responses by IL-10-treated dendritic cells: differences from hydrocortisone-treated dendritic cells. J Allergy Clin Immunol (2001) 108:242–9. doi:10.1067/mai.2001.117177
102. Lutz MB, Schuler G. Immature, semi-mature and fully mature dendritic cells: which signals induce tolerance or immunity? Trends Immunol (2002) 23:445–9. doi:10.1016/S1471-4906(02)02281-0
103. Reis e Sousa C. Dendritic cells in a mature age. Nat Rev Immunol (2006) 6:476–83. doi:10.1038/nri1845
104. Steinbrink K, Graulich E, Kubsch S, Knop J, Enk AH. CD4(+) and CD8(+) anergic T cells induced by interleukin-10-treated human dendritic cells display antigen-specific suppressor activity. Blood (2002) 99:2468–76. doi:10.1182/blood.V99.7.2468
105. Jonuleit H, Schmitt E, Schuler G, Knop J, Enk AH. Induction of interleukin 10-producing, nonproliferating CD4(+) T cells with regulatory properties by repetitive stimulation with allogeneic immature human dendritic cells. J Exp Med (2000) 192:1213–22. doi:10.1084/jem.192.9.1213
106. Sato K, Yamashita N, Baba M, Matsuyama T. Modified myeloid dendritic cells act as regulatory dendritic cells to induce anergic and regulatory T cells. Blood (2003) 101:3581–9. doi:10.1182/blood-2002-09-2712
107. De Smedt T, Van Mechelen M, De Becker G, Urbain J, Leo O, Moser M. Effect of interleukin-10 on dendritic cell maturation and function. Eur J Immunol (1997) 27:1229–35. doi:10.1002/eji.1830270526
108. Liu L, Rich BE, Inobe J, Chen W, Weiner HL. Induction of Th2 cell differentiation in the primary immune response: dendritic cells isolated from adherent cell culture treated with IL-10 prime naive CD4+ T cells to secrete IL-4. Int Immunol (1998) 10:1017–26. doi:10.1093/intimm/10.8.1017
109. Morel E, Bellón T. HLA class I molecules regulate IFN-gamma production induced in NK cells by target cells, viral products, or immature dendritic cells through the inhibitory receptor ILT2/CD85j. J Immunol (2008) 181:2368–81. doi:10.4049/jimmunol.181.4.2368
110. Young NT, Waller ECP, Patel R, Roghanian A, Austyn JM, Trowsdale J. The inhibitory receptor LILRB1 modulates the differentiation and regulatory potential of human dendritic cells. Blood (2008) 111:3090–6. doi:10.1182/blood-2007-05-089771
111. Adams VC, Hunt JRF, Martinelli R, Palmer R, Rook GAW, Brunet LR. Mycobacterium vaccae induces a population of pulmonary CD11c+ cells with regulatory potential in allergic mice. Eur J Immunol (2004) 34:631–8. doi:10.1002/eji.200324659
112. Schülke S, Vogel L, Junker A-C, Hanschmann K-M, Flaczyk A, Vieths S, et al. A fusion protein consisting of the vaccine adjuvant monophosphoryl lipid A and the allergen ovalbumin boosts allergen-specific Th1, Th2, and Th17 responses in vitro. J Immunol Res (2016) 2016:4156456–8. doi:10.1155/2016/4156456
113. Amberbir A, Medhin G, Erku W, Alem A, Simms R, Robinson K, et al. Effects of Helicobacter pylori, geohelminth infection and selected commensal bacteria on the risk of allergic disease and sensitization in 3-year-old Ethiopian children. Clin Exp Allergy (2011) 41:1422–30. doi:10.1111/j.1365-2222.2011.03831.x
114. Chen Y, Blaser MJ. Helicobacter pylori colonization is inversely associated with childhood asthma. J Infect Dis (2008) 198:553–60. doi:10.1086/590158
115. Arnold IC, Dehzad N, Reuter S, Martin H, Becher B, Taube C, et al. Helicobacter pylori infection prevents allergic asthma in mouse models through the induction of regulatory T cells. J Clin Invest (2011) 121:3088–93. doi:10.1172/JCI45041
116. Chen Q, Davidson TS, Huter EN, Shevach EM. Engagement of TLR2 does not reverse the suppressor function of mouse regulatory T cells, but promotes their survival. J Immunol (2009) 183:4458–66. doi:10.4049/jimmunol.0901465
117. DePaolo RW, Kamdar K, Khakpour S, Sugiura Y, Wang W, Jabri B. A specific role for TLR1 in protective T(H)17 immunity during mucosal infection. J Exp Med (2012) 209:1437–44. doi:10.1084/jem.20112339
118. Taylor RC, Richmond P, Upham JW. Toll-like receptor 2 ligands inhibit TH2 responses to mite allergen. J Allergy Clin Immunol (2006) 117:1148–54. doi:10.1016/j.jaci.2006.02.014
119. Khan S, Bijker MS, Weterings JJ, Tanke HJ, Adema GJ, van Hall T, et al. Distinct uptake mechanisms but similar intracellular processing of two different toll-like receptor ligand-peptide conjugates in dendritic cells. J Biol Chem (2007) 282:21145–59. doi:10.1074/jbc.M701705200
120. Kundi M. New hepatitis B vaccine formulated with an improved adjuvant system. Expert Rev Vaccines (2007) 6:133–40. doi:10.1586/14760584.6.2.133
121. Agnandji ST, Lell B, Soulanoudjingar SS, Fernandes JF, Abossolo BP, Conzelmann C, et al. First results of phase 3 trial of RTS,S/AS01 malaria vaccine in African children. N Engl J Med (2011) 365:1863–75. doi:10.1056/NEJMoa1102287
122. GlaxoSmithKline Vaccine HPV-007 Study GroupRomanowski B, de Borba PC, Naud PS, Roteli-Martins CM, De Carvalho NS, et al. Sustained efficacy and immunogenicity of the human papillomavirus (HPV)-16/18 AS04-adjuvanted vaccine: analysis of a randomised placebo-controlled trial up to 6.4 years. Lancet (2009) 374:1975–85. doi:10.1016/S0140-6736(09)61567-1
123. Puggioni F, Durham SR, Francis JN. Monophosphoryl lipid A (MPLR)* promotes allergen-induced immune deviation in favour of Th1 responses. Allergy (2005) 60:678–84. doi:10.1111/j.1398-9995.2005.00762.x
124. Parronchi P, Brugnolo F, Annunziato F, Manuelli C, Sampognaro S, Mavilia C, et al. Phosphorothioate oligodeoxynucleotides promote the in vitro development of human allergen-specific CD4+ T cells into Th1 effectors. J Immunol (1999) 163:5946–53.
125. Bohle B, Jahn-Schmid B, Maurer D, Kraft D, Ebner C. Oligodeoxynucleotides containing CpG motifs induce IL-12, IL-18 and IFN-gamma production in cells from allergic individuals and inhibit IgE synthesis in vitro. Eur J Immunol (1999) 29:2344–53. doi:10.1002/(SICI)1521-4141(199907)29:07<2344::AID-IMMU2344>3.0.CO;2-R
126. Horner AA, Widhopf GF, Burger JA, Takabayashi K, Cinman N, Ronaghy A, et al. Immunostimulatory DNA inhibits IL-4-dependent IgE synthesis by human B cells. J Allergy ClinImmunol (2001) 108:417–23. doi:10.1067/mai.2001.117795
127. Marshall JD, Abtahi S, Eiden JJ, Tuck S, Milley R, Haycock F, et al. Immunostimulatory sequence DNA linked to the Amb a 1 allergen promotes T(H)1 cytokine expression while downregulating T(H)2 cytokine expression in PBMCs from human patients with ragweed allergy. J Allergy ClinImmunol (2001) 108:191–7. doi:10.1067/mai.2001.116984
128. Tighe H, Takabayashi K, Schwartz D, Van Nest G, Tuck S, Eiden JJ, et al. Conjugation of immunostimulatory DNA to the short ragweed allergen Amb a 1 enhances its immunogenicity and reduces its allergenicity. J Allergy ClinImmunol (2000) 106:124–34. doi:10.1067/mai.2000.107927
129. Horner AA, Takabayashi K, Beck L, Sharma B, Zubeldia J, Baird S, et al. Optimized conjugation ratios lead to allergen immunostimulatory oligodeoxynucleotide conjugates with retained immunogenicity and minimal anaphylactogenicity. J Allergy ClinImmunol (2002) 110:413–20. doi:10.1067/mai.2002.126660
130. Powell JD, Pollizzi KN, Heikamp EB, Horton MR. Regulation of immune responses by mTOR. Annu Rev Immunol (2012) 30:39–68. doi:10.1146/annurev-immunol-020711-075024
131. Säemann MD, Haidinger M, Hecking M, Hörl WH, Weichhart T. The multifunctional role of mTOR in innate immunity: implications for transplant immunity. Am J Transplant (2009) 9:2655–61. doi:10.1111/j.1600-6143.2009.02832.x
132. Bubnoff Von D, Scheler M, Wilms H, Fimmers R, Bieber T. Identification of IDO-positive and IDO-negative human dendritic cells after activation by various proinflammatory stimuli. J Immunol (2011) 186:6701–9. doi:10.4049/jimmunol.1003151
133. Lyakh LA, Sanford M, Chekol S, Young HA, Roberts AB. TGF-beta and vitamin D3 utilize distinct pathways to suppress IL-12 production and modulate rapid differentiation of human monocytes into CD83+ dendritic cells. J Immunol (2005) 174:2061–70. doi:10.4049/jimmunol.174.4.2061
134. Ciesek S, Ringe BP, Strassburg CP, Klempnauer J, Manns MP, Wedemeyer H, et al. Effects of cyclosporine on human dendritic cell subsets. Transplant Proc (2005) 37:20–4. doi:10.1016/j.transproceed.2004.11.055
135. Delgado M, Chorny A, Ganea D, Gonzalez-Rey E. Vasoactive intestinal polypeptide induces regulatory dendritic cells that prevent acute graft versus host disease and leukemia relapse after bone marrow transplantation. Ann N Y Acad Sci (2006) 1070:226–32. doi:10.1196/annals.1317.019
Keywords: dendritic cells, interleukin-10, allergy, T helper 2, dendritic cell vaccine
Citation: Schülke S (2018) Induction of Interleukin-10 Producing Dendritic Cells As a Tool to Suppress Allergen-Specific T Helper 2 Responses. Front. Immunol. 9:455. doi: 10.3389/fimmu.2018.00455
Received: 04 January 2018; Accepted: 20 February 2018;
Published: 19 March 2018
Edited by:
Nestor González Roldán, Allergobiochemie, Forschungszentrum Borstel, GermanyReviewed by:
Angela Bonura, Consiglio Nazionale Delle Ricerche (CNR), ItalyRaymond P. Donnelly, United States Food and Drug Administration, United States
Copyright: © 2018 Schülke. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Stefan Schülke, c3RlZmFuLnNjaHVlbGtlJiN4MDAwNDA7cGVpLmRl