Skip to main content

REVIEW article

Front. Immunol., 17 December 2015
Sec. T Cell Biology
This article is part of the Research Topic Setting the path – How qualitative and quantitative differences in TCR-pMHC interactions regulate T cell immune responses View all 12 articles

Naïve T Cell Homeostasis Regulated by Stress Responses and TCR Signaling

\r\n      \r\nDaisuke Kamimura,,Daisuke Kamimura1,2,3Toru Atsumi,,Toru Atsumi1,2,3Andrea StofkovaAndrea Stofkova1Naoki NishikawaNaoki Nishikawa1Takuto Ohki,,Takuto Ohki1,2,3Hironao Suzuki,,Hironao Suzuki1,2,3Kokichi Katsunuma,Kokichi Katsunuma2,3Jing-jing Jiang,,Jing-jing Jiang1,2,3Hidenori Bando,,Hidenori Bando1,2,3Jie Meng,,Jie Meng1,2,3Lavannya Sabharwal,,Lavannya Sabharwal1,2,3Hideki Ogura,,Hideki Ogura1,2,3Toshio HiranoToshio Hirano4Yasunobu Arima,,Yasunobu Arima1,2,3Masaaki Murakami,,*\r\n   Masaaki Murakami1,2,3*
  • 1Division of Molecular Neuroimmunology, Institute for Genomic Medicine, Graduate School of Medicine, Hokkaido University, Sapporo, Japan
  • 2Laboratory of Developmental Immunology, WPI Immunology Frontier Research Center, Graduate School of Frontier Biosciences, Osaka University, Suita, Japan
  • 3Laboratory of Developmental Immunology, WPI Immunology Frontier Research Center, Graduate School of Medicine, Osaka University, Suita, Japan
  • 4Osaka University, Suita, Japan

The survival of naïve T cells is believed to require signals from TCR–pMHC interactions and cytokines such as IL-7. In contrast, signals that negatively impact naïve T cell survival are less understood. We conducted a forward genetic screening of mice and found a mutant mouse line with reduced number of naïve T cells (T-Red mice). T-Red mice have a point mutation in the Kdelr1 gene, and their naïve T cells show enhanced integrated stress response (ISR), which eventually induces their apoptosis. Therefore, naïve T cells require a KDEL receptor-mediated mechanism that efficiently relieves cellular stress for their survival in vivo. Interestingly, naïve T cells expressing TCR with higher affinity/avidity to self-antigens survive in T-Red mice, suggesting the possible link between TCR-mediated survival and ISR-induced apoptosis. In this article, we discuss the regulation of naïve T cell homeostasis, keeping special attention on the ISR and TCR signal.

Positive Signals for T Cell Homeostasis: Lessons from Previous Studies

T cell numbers in the periphery are almost constant, even though millions of naïve T cells are generated from the thymus daily. This homeostasis likely requires strict regulation. Additionally, naïve T cells have a relatively long half-life of approximately >50 days, and memory T cells show basal turnover to survive longer in the periphery (13). Survival signals for naïve T cells include TCR–pMHC interactions and cytokines such as IL-7, whereas the homeostasis of memory T cells is largely dependent on cytokines such as IL-2 and IL-15 along with IL-7 (4).

When peripheral naïve and memory T cells are decreased due to involution of the thymus by aging, infection, or irradiation, the remaining T cells start to proliferate, a phenomenon called homeostatic proliferation. At the molecular level, homeostatic proliferation is induced by TCR signaling and/or cytokines (5). Homeostatic proliferation sometimes plays a role in the activation of autoreactive T cells, causing several autoimmune diseases (6, 7). This homeostatic proliferation can be experimentally induced by a sublethal dose of irradiation in mice called lymphopenia-induced proliferation (LIP). LIP has greatly contributed to the understanding of the mechanism of T cell homeostasis in vivo, which otherwise takes a long time to study due to the slow turnover of these cells. LIP of naïve CD4+ and CD8+ T cells does not occur in the absence of TCR–pMHC interactions or IL-7 (5, 8), and genetic deficiency of IL-7 in host animals abrogates LIP (9). Compared to the involvement of cytokines, the contribution of TCR signaling to T cell homeostasis is more complicated. Homeostatic proliferation induced by TCR–pMHC interactions involves clonal competition among T cells, because LIP of monoclonal TCR transgenic (Tg) T cells is inhibited in TCR Tg mice that have the same monoclonal TCR (1013). These observations suggest that not all T cells equally proliferate under LIP; those that have higher TCR affinity to self-antigens receive more TCR signaling to outcompete T cells expressing TCR with lower self-affinity in the periphery. Measurement of TCR avidity to self-peptide–MHC complexes under normal polyclonal conditions is challenging. However, it is known that CD5 can be a surrogate marker that reports the TCR signal strength in T cells. CD5 is a negative regulator of TCR signaling and whose expression correlates with the avidity of TCR (14, 15). Surface CD5 levels in T cells positively correlate with the phosphorylation levels of the CD3ζ chain, which is a proximal signal event after TCR ligation (15), and with Nur77 expression, which is an early target gene of TCR signaling (16). Indeed, LIP of CD8+ T cells derived from TCR Tg mice with high CD5 expression, such as OT-I cells, is faster than that of CD8+ T cells derived from other TCR Tg mice with lower CD5 expression (17). Thus, TCR affinity to self-pMHC contributes to the degree of survival signals that T cells receive. It is known that IL-7-mediated cell death is suppressed in CD8+ T cells by TCR signaling via self-pMHC. For example, IL-7Rα expression on T cells bearing low-affinity TCR requires TGF-β signaling (18). Continuous IL-7 signaling triggers cytokine-induced cell death in CD8+ T cells, while homeostatic engagement of TCR interrupts IL-7-mediated cell death. Consistent with these properties, CD8+ T cells with insufficient TCR affinity via self-pMHC induce IL-7-mediated cell death (19). These studies clearly suggest that T cell survival is tightly regulated by the TCR signal via self-pMHC and cytokines in vivo.

T-Red Mice: Mutant Animals with Enhanced Naïve T Cell Death

To obtain more insight into T cell survival and death, we conducted an N-ethyl-N-nitrosourea (ENU) mutagenesis experiment and searched for mutant mice with unusual naïve vs. memory phenotype T cell ratios in peripheral blood. After the establishment of a mutant line that has excess CD44High memory-phenotype T cells in peripheral blood (20, 21), we enumerated T cell numbers in the spleen and lymph nodes, revealing that CD44High memory-phenotype T cells were not increased, but rather CD44Lo naïve T cells were significantly decreased. Intriguingly, the phenotype of cellular loss was selective to naïve T cells because other immune cells, such as γδT cells, neutrophils, and dendritic cells, were not significantly reduced. Therefore, we named this mutant T-Red (naïve T cell reducing) mice (21). Compared to WT naïve T cells, the survival of T-Red naïve T cells is impaired in vitro and in vivo. Several T cell-dependent immune responses, such as collagen-induced arthritis and T cell responses to bacterial infection, are severely diminished in T-Red mice (21). Importantly, this mutant mouse is suitable for the investigation of naïve T-cell homeostasis in vivo.

KDEL Receptor 1 (Kdelr1) is the Gene Responsible for T-Red Mice

To identify the gene responsible for the ENU mutants, T-Red mice (C57BL/6 background) were crossed with mice on a different genetic background (C3H/He). The resulting F1 hybrids were intercrossed, and the CD44High T-Red phenotype was screened out in the F2 generation. Positional cloning using SNP markers detecting C57BL/6 genomic regions and DNA sequence analysis of T-Red mice on the mixed background identified an amino acid substituting point mutation (S123P) in the gene encoding KDEL receptor 1 (Kdelr1) (21). It is reported that the mutated residue, serine 123, of Kdelr1 is involved in the receptor’s conformation (22), suggesting that the T-Red mutation causes dysfunction of Kdelr1. Because multiple mutations are inevitably induced in the ENU mutant, a rescue experiment and generation of Kdelr1-deficient mice including CD4 T cell-specific Kdelr1 KO mice were performed, formally demonstrating that Kdelr1 is the responsible gene for the T-Red phenotype (21).

The KDEL receptor was originally identified as a chaperone retrieval receptor that recovers soluble endoplasmic reticulum (ER)-resident chaperones from the cis-Golgi. This Golgi-to-ER retrograde transport requires the binding of KDEL receptors with a KDEL motif localized at the C-terminal of the ER chaperones (2325). Examples of actual and potential KDEL receptor ligands having the KDEL motif or its variants include Bip (Grp78), calreticulin, Hsp90b1, and several FK506-binding proteins (26). In mammals, there are three KDEL receptors, Kdelr1, Kdelr2, and Kdelr3, all of which are localized around ER and Golgi (27). A role for KDEL receptors beyond chaperone retrieval was also reported, as chaperone-bound KDEL receptors trigger the activation of Src family kinases at the Golgi complex to generate intracellular signaling cascades that coordinate the secretory pathway (27, 28). Furthermore, consistent with their ER-Golgi localization, KDEL receptors are involved in ER stress responses (29). Using T-Red mice, we found a novel role for Kdelr1 in naïve T cell homeostasis (Figure 1).

FIGURE 1
www.frontiersin.org

Figure 1. The Kdelr1–PP1 axis is required for naïve T cell survival. In WT naïve T cells, PP1 associated with Kdelr1 exhibits optimal phosphatase activity to prevent unwanted persistence of ISR. In T-Red naïve T cells, however, Kdelr1 dysfunction results in reduced PP1 activity, leading to prolonged ISR and apoptosis. Endogenous factors that cause ISR in vivo remain unidentified.

Kdelr1–PP1 Axis: A New Mechanism That Alleviates Cellular Stresses in Naïve T Cells

Transcriptome analysis provided a clue about how Kdelr1 regulates the survival of naïve T cells. In T-Red naïve CD4+ and CD8+ T cells, gene expressions related to the integrated stress response (ISR), including those of Asns (asparagine synthetase), Trib3, Chop, and Vegfa, were significantly increased. The expression of Bim, which is a proapoptotic factor critically involved in T cell death, is controlled by Chop (30), suggesting that stress increases Bim expression in T-Red mice. Consistent with this notion, Bim levels are elevated in T-Red mice.

Integrated stress response is a cellular response that is induced by various types of stress signals, including ER stress, amino acid deprivation, infection with double-stranded RNA viruses, heme deficiency, and oxidative stress. All these stressors increase the phosphorylation at serine 51 of the α subunit of eukaryotic initiation factor 2 (eIF2α). Thus, eIF2α phosphorylation is a key event to triggering ISR (3134). Indeed, T-Red naïve T cells have excess phosphorylation of eIF2α (21). We therefore considered how the excessive phosphorylation of eIF2α is regulated. It has been reported that phosphorylation of eIF2α is mediated by four kinases: double-stranded RNA-dependent protein kinase R (PKR), RNA-dependent protein kinase-like ER kinase (PERK), general control non-repressed 2 (GCN2), and heme-regulated eIF2α kinase (HRI) (35). However, the activation status of these four kinases was not enhanced in T-Red naïve T cells, suggesting that Kdelr1 is not involved in their regulation. The phosphorylated form of eIF2α is efficiently dephosphorylated by protein phosphatase 1 (PP1) to complete ISR. Importantly, PP1 activity in T-Red naïve T cells was reduced compared with WT naïve T cells. Additionally, the phosphorylation of eIF2α is evident in freshly isolated WT naïve T cells, suggesting the existence of certain stressor(s) for naïve T cells in vivo (21). Moreover, Kdelr1 associated with PP1, whereas mutant Kdelr1 did not. These results suggested that Kdelr1 is required for optimal PP1 activity to dephosphorylate eIF2α in naïve T cells under stress. Thus, the Kdelr1–PP1 axis regulates naïve T cell death, which is induced by ISR in vivo (Figure 2) (21).

FIGURE 2
www.frontiersin.org

Figure 2. Strong TCR-mediated signals alleviate ISR in T-Red naïve T cells. T-Red naïve T cells expressing TCR with relatively high self-affinity are resistant to enhanced ISR caused by Kdelr1 dysfunction. Further studies are required to reveal the specific TCR signaling that counteracts ISR.

Strong TCR-Mediated Signals Alleviate ISR in vivo

As described above, T-Red mice show the increased CD44High phenotype in T cells due to a reduction of naïve T cell numbers. Interestingly, the CD44High phenotype became normal when T-Red mice were bred with TCR Tg mice such as OT-I and P14 mice. Absolute cell numbers of TCR Tg T cells were also the same between T-Red/TCR Tg and control TCR Tg mice (21). OT-I TCR Tg mice have TCR, which recognizes an ovalbumin (OVA) peptide on MHC class I-restricted TCR, and P14 TCR Tg mice express TCR, which recognize a lymphocytic choriomeningitis virus peptide on MHC class I, although specific endogenous antigen peptides are not known in either TCR Tg cases. However, it is known that both transgenic TCRs have relatively high self-peptide affinity (16, 17). Therefore, we hypothesized that naïve T cells that receive relatively stronger TCR signals due to higher self-affinity might be resistant to enhanced ISR. Consistent with this idea, the surviving naïve T cells in T-Red mice express significantly higher levels of CD5 (14, 15). In addition, a tetramer dissociation assay using peptides derived from self-antigens confirmed higher TCR affinity/avidity in T-Red naïve T cells. These results suggest a novel link between TCR signal strength and ISR-mediated apoptosis during steady state in naïve T cells.

These findings prompted us to examine the possibility of whether TCR stimulations with a panel of peptide ligands having various affinities to TCR may result in different responses between WT and T-Red naïve T cells in vivo and in vitro. We used ovalbumin (OVA)-altered peptide ligands (APL) as a model antigen (3638) to stimulate control OT-I and T-Red/OT-I T cells and assessed the survival response in vitro and proliferation response in vivo. We found that weak TCR stimulation decreases the survival and proliferation of T-Red naïve T cells, whereas stimulation with ligands having higher affinity has no such effect (39). These results suggest that a strong physiological TCR signal suppresses IRS, whereas a weak one does not (Figure 2).

Future Directions

Even in steady state, in vivo T cells have a certain level of eIF2α phosphorylation, suggesting that they receive a certain level of stress. However, the endogenous stressors for T cells particularly in vivo are unknown. Because the thymus from mice deficient in PERK has reduced levels of eIF2α phosphorylation, at least in the pancreas (40), it might be possible that some activators of eIF2α kinases are stressors in naïve T cells. In summary, we identified that the Kdelr1–PP1 axis plays a beneficial role for naïve T cells to resolve cellular stress in vivo. Our study also revealed that TCR signaling can counteract ISR in naïve T cells. A molecular mechanism behind TCR signaling and the resistance to ISR should be elucidated in the near future.

Author Contributions

All authors contributed to write and elaborate the manuscript.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

We thank the excellent assistance by Ms. Chiemi Nakayama and Ms. Satomi Fukumoto (Hokkaido University, Sapporo, Japan). This work was supported by KAKENHI (DK, YA, TA, MM, and TH), Takeda Science Foundation (MM), Institute for Fermentation Osaka (MM), Mitsubishi Foundation (MM), Mochida Memorial Foundation for Medical and Pharmaceutical Research (DK), Suzuken Memorial Foundation (YA), Japan Prize Foundation (YA), Ono Medical Research Foundation (YA), Kanzawa Medical Research Foundation (YA), Kishimoto Foundation (YA), Nagao Takeshi Research Foundation (YA), Tokyo Medical Research Foundation (MM and YA), JSPS Postdoctoral Fellowship for Foreign Researchers (AS), the JST-CREST program (MM and TH), and the Osaka Foundation for the Promotion of Clinical Immunology (MM).

References

1. Hataye J, Moon JJ, Khoruts A, Reilly C, Jenkins MK. Naive and memory CD4+ T cell survival controlled by clonal abundance. Science (2006) 312:114–6. doi: 10.1126/science.1124228

PubMed Abstract | CrossRef Full Text | Google Scholar

2. Surh CD, Sprent J. Homeostasis of naive and memory T cells. Immunity (2008) 29:848–62. doi:10.1016/j.immuni.2008.11.002

CrossRef Full Text | Google Scholar

3. Den Braber I, Mugwagwa T, Vrisekoop N, Westera L, Mogling R, De Boer AB, et al. Maintenance of peripheral naive T cells is sustained by thymus output in mice but not humans. Immunity (2012) 36:288–97. doi:10.1016/j.immuni.2012.02.006

PubMed Abstract | CrossRef Full Text | Google Scholar

4. Marrack P, Kappler J. Control of T cell viability. Annu Rev Immunol (2004) 22:765–87. doi:10.1146/annurev.immunol.22.012703.104554

PubMed Abstract | CrossRef Full Text | Google Scholar

5. Sprent J, Surh CD. Normal T cell homeostasis: the conversion of naive cells into memory-phenotype cells. Nat Immunol (2011) 12:478–84. doi:10.1038/ni.2018

PubMed Abstract | CrossRef Full Text | Google Scholar

6. King C, Ilic A, Koelsch K, Sarvetnick N. Homeostatic expansion of T cells during immune insufficiency generates autoimmunity. Cell (2004) 117:265–77. doi:10.1016/S0092-8674(04)00335-6

PubMed Abstract | CrossRef Full Text | Google Scholar

7. Sawa S, Kamimura D, Jin GH, Morikawa H, Kamon H, Nishihara M, et al. Autoimmune arthritis associated with mutated interleukin (IL)-6 receptor gp130 is driven by STAT3/IL-7-dependent homeostatic proliferation of CD4+ T cells. J Exp Med (2006) 203:1459–70. doi:10.1084/jem.20052187

PubMed Abstract | CrossRef Full Text | Google Scholar

8. Takada K, Jameson SC. Naive T cell homeostasis: from awareness of space to a sense of place. Nat Rev Immunol (2009) 9:823–32. doi:10.1038/nri2657

PubMed Abstract | CrossRef Full Text | Google Scholar

9. Tan JT, Dudl E, Leroy E, Murray R, Sprent J, Weinberg KI, et al. IL-7 is critical for homeostatic proliferation and survival of naive T cells. Proc Natl Acad Sci U S A (2001) 98:8732–7. doi:10.1073/pnas.161126098

PubMed Abstract | CrossRef Full Text | Google Scholar

10. Moses CT, Thorstenson KM, Jameson SC, Khoruts A. Competition for self ligands restrains homeostatic proliferation of naive CD4 T cells. Proc Natl Acad Sci U S A (2003) 100:1185–90. doi:10.1073/pnas.0334572100

PubMed Abstract | CrossRef Full Text | Google Scholar

11. Troy AE, Shen H. Cutting edge: homeostatic proliferation of peripheral T lymphocytes is regulated by clonal competition. J Immunol (2003) 170:672–6. doi:10.4049/jimmunol.170.2.672

PubMed Abstract | CrossRef Full Text | Google Scholar

12. Ge Q, Bai A, Jones B, Eisen HN, Chen J. Competition for self-peptide-MHC complexes and cytokines between naive and memory CD8+ T cells expressing the same or different T cell receptors. Proc Natl Acad Sci U S A (2004) 101:3041–6. doi:10.1073/pnas.0307339101

PubMed Abstract | CrossRef Full Text | Google Scholar

13. Leitao C, Freitas AA, Garcia S. The role of TCR specificity and clonal competition during reconstruction of the peripheral T cell pool. J Immunol (2009) 182:5232–9. doi:10.4049/jimmunol.0804071

PubMed Abstract | CrossRef Full Text | Google Scholar

14. Tarakhovsky A, Kanner SB, Hombach J, Ledbetter JA, Muller W, Killeen N, et al. A role for CD5 in TCR-mediated signal transduction and thymocyte selection. Science (1995) 269:535–7. doi:10.1126/science.7542801

PubMed Abstract | CrossRef Full Text | Google Scholar

15. Mandl JN, Monteiro JP, Vrisekoop N, Germain RN. T cell-positive selection uses self-ligand binding strength to optimize repertoire recognition of foreign antigens. Immunity (2013) 38:263–74. doi:10.1016/j.immuni.2012.09.011

PubMed Abstract | CrossRef Full Text | Google Scholar

16. Fulton RB, Hamilton SE, Xing Y, Best JA, Goldrath AW, Hogquist KA, et al. The TCR’s sensitivity to self peptide-MHC dictates the ability of naive CD8(+) T cells to respond to foreign antigens. Nat Immunol (2015) 16:107–17. doi:10.1038/ni.3043

PubMed Abstract | CrossRef Full Text | Google Scholar

17. Kieper WC, Burghardt JT, Surh CD. A role for TCR affinity in regulating naive T cell homeostasis. J Immunol (2004) 172:40–4. doi:10.4049/jimmunol.172.1.40

PubMed Abstract | CrossRef Full Text | Google Scholar

18. Ouyang W, Oh SA, Ma Q, Bivona MR, Zhu J, Li MO. TGF-beta cytokine signaling promotes CD8+ T cell development and low-affinity CD4+ T cell homeostasis by regulation of interleukin-7 receptor alpha expression. Immunity (2013) 39:335–46. doi:10.1016/j.immuni.2013.07.016

CrossRef Full Text | Google Scholar

19. Kimura MY, Pobezinsky LA, Guinter TI, Thomas J, Adams A, Park JH, et al. IL-7 signaling must be intermittent, not continuous, during CD8(+) T cell homeostasis to promote cell survival instead of cell death. Nat Immunol (2013) 14:143–51. doi:10.1038/ni.2494

CrossRef Full Text | Google Scholar

20. Papathanasiou P, Goodnow CC. Connecting mammalian genome with phenome by ENU mouse mutagenesis: gene combinations specifying the immune system. Annu Rev Genet (2005) 39:241–62. doi:10.1146/annurev.genet.39.110304.095817

PubMed Abstract | CrossRef Full Text | Google Scholar

21. Kamimura D, Katsunuma K, Arima Y, Atsumi T, Jiang JJ, Bando H, et al. KDEL receptor 1 regulates T-cell homeostasis via PP1 that is a key phosphatase for ISR. Nat Commun (2015) 6:7474. doi:10.1038/ncomms8474

CrossRef Full Text | Google Scholar

22. Scheel AA, Pelham HR. Identification of amino acids in the binding pocket of the human KDEL receptor. J Biol Chem (1998) 273:2467–72. doi:10.1074/jbc.273.4.2467

PubMed Abstract | CrossRef Full Text | Google Scholar

23. Lewis MJ, Pelham HR. A human homologue of the yeast HDEL receptor. Nature (1990) 348:162–3. doi:10.1038/348162a0

PubMed Abstract | CrossRef Full Text | Google Scholar

24. Semenza JC, Hardwick KG, Dean N, Pelham HR. ERD2, a yeast gene required for the receptor-mediated retrieval of luminal ER proteins from the secretory pathway. Cell (1990) 61:1349–57. doi:10.1016/0092-8674(90)90698-E

PubMed Abstract | CrossRef Full Text | Google Scholar

25. Lewis MJ, Pelham HR. Ligand-induced redistribution of a human KDEL receptor from the Golgi complex to the endoplasmic reticulum. Cell (1992) 68:353–64. doi:10.1016/0092-8674(92)90476-S

PubMed Abstract | CrossRef Full Text | Google Scholar

26. Raykhel I, Alanen H, Salo K, Jurvansuu J, Nguyen VD, Latva-Ranta M, et al. A molecular specificity code for the three mammalian KDEL receptors. J Cell Biol (2007) 179:1193–204. doi:10.1083/jcb.200705180

PubMed Abstract | CrossRef Full Text | Google Scholar

27. Capitani M, Sallese M. The KDEL receptor: new functions for an old protein. FEBS Lett (2009) 583:3863–71. doi:10.1016/j.febslet.2009.10.053

PubMed Abstract | CrossRef Full Text | Google Scholar

28. Pulvirenti T, Giannotta M, Capestrano M, Capitani M, Pisanu A, Polishchuk RS, et al. A traffic-activated Golgi-based signalling circuit coordinates the secretory pathway. Nat Cell Biol (2008) 10:912–22. doi:10.1038/ncb1751

PubMed Abstract | CrossRef Full Text | Google Scholar

29. Yamamoto K, Hamada H, Shinkai H, Kohno Y, Koseki H, Aoe T. The KDEL receptor modulates the endoplasmic reticulum stress response through mitogen-activated protein kinase signaling cascades. J Biol Chem (2003) 278:34525–32. doi:10.1074/jbc.M304188200

PubMed Abstract | CrossRef Full Text | Google Scholar

30. Puthalakath H, O’reilly LA, Gunn P, Lee L, Kelly PN, Huntington ND, et al. ER stress triggers apoptosis by activating BH3-only protein Bim. Cell (2007) 129:1337–49. doi:10.1016/j.cell.2007.04.027

PubMed Abstract | CrossRef Full Text | Google Scholar

31. Williams BR. PKR; a sentinel kinase for cellular stress. Oncogene (1999) 18:6112–20. doi:10.1038/sj.onc.1203127

PubMed Abstract | CrossRef Full Text | Google Scholar

32. Harding HP, Novoa I, Zhang Y, Zeng H, Wek R, Schapira M, et al. Regulated translation initiation controls stress-induced gene expression in mammalian cells. Mol Cell (2000) 6:1099–108. doi:10.1016/S1097-2765(00)00108-8

PubMed Abstract | CrossRef Full Text | Google Scholar

33. Han AP, Yu C, Lu L, Fujiwara Y, Browne C, Chin G, et al. Heme-regulated eIF2alpha kinase (HRI) is required for translational regulation and survival of erythroid precursors in iron deficiency. EMBO J (2001) 20:6909–18. doi:10.1093/emboj/20.23.6909

PubMed Abstract | CrossRef Full Text | Google Scholar

34. Lu L, Han AP, Chen JJ. Translation initiation control by heme-regulated eukaryotic initiation factor 2alpha kinase in erythroid cells under cytoplasmic stresses. Mol Cell Biol (2001) 21:7971–80. doi:10.1128/MCB.21.23.7971-7980.2001

PubMed Abstract | CrossRef Full Text | Google Scholar

35. Donnelly N, Gorman AM, Gupta S, Samali A. The eIF2alpha kinases: their structures and functions. Cell Mol Life Sci (2013) 70:3493–511. doi:10.1007/s00018-012-1252-6

CrossRef Full Text | Google Scholar

36. Daniels MA, Teixeiro E, Gill J, Hausmann B, Roubaty D, Holmberg K, et al. Thymic selection threshold defined by compartmentalization of Ras/MAPK signalling. Nature (2006) 444:724–9. doi:10.1038/nature05269

PubMed Abstract | CrossRef Full Text | Google Scholar

37. Zehn D, Lee SY, Bevan MJ. Complete but curtailed T-cell response to very low-affinity antigen. Nature (2009) 458:211–4. doi:10.1038/nature07657

PubMed Abstract | CrossRef Full Text | Google Scholar

38. Stepanek O, Prabhakar AS, Osswald C, King CG, Bulek A, Naeher D, et al. Coreceptor scanning by the T cell receptor provides a mechanism for T cell tolerance. Cell (2014) 159:333–45. doi:10.1016/j.cell.2014.08.042

PubMed Abstract | CrossRef Full Text | Google Scholar

39. Kamimura D, Arima Y, Tsuruoka M, Jiang JJ, Bando H, Meng J, et al. Strong TCR-mediated signals suppress integrated stress responses induced by KDELR1 deficiency in naive T cells. Int Immunol (2015). doi:10.1093/intimm/dxv059

CrossRef Full Text | Google Scholar

40. Harding HP, Zeng H, Zhang Y, Jungries R, Chung P, Plesken H, et al. Diabetes mellitus and exocrine pancreatic dysfunction in perk-/- mice reveals a role for translational control in secretory cell survival. Mol Cell (2001) 7:1153–63. doi:10.1016/S1097-2765(01)00264-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Keywords: T cell homeostasis, stress response, TCR signaling, naive T cells, mutagenesis

Citation: Kamimura D, Atsumi T, Stofkova A, Nishikawa N, Ohki T, Suzuki H, Katsunuma K, Jiang J-j, Bando H, Meng J, Sabharwal L, Ogura H, Hirano T, Arima Y and Murakami M (2015) Naïve T Cell Homeostasis Regulated by Stress Responses and TCR Signaling. Front. Immunol. 6:638. doi: 10.3389/fimmu.2015.00638

Received: 17 September 2015; Accepted: 04 December 2015;
Published: 17 December 2015

Edited by:

Dietmar Zehn, Swiss Vaccine Research Institute, Switzerland

Reviewed by:

Paula M. Oliver, University of Pennsylvania, USA
Matthew Williams, University of Utah, USA

Copyright: © 2015 Kamimura, Atsumi, Stofkova, Nishikawa, Ohki, Suzuki, Katsunuma, Jiang, Bando, Meng, Sabharwal, Ogura, Hirano, Arima and Murakami. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Masaaki Murakami, bXVyYWthbWkmI3gwMDA0MDtpZ20uaG9rdWRhaS5hYy5qcA==

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.