Fenglin Deng
Fanrong Zeng
Gareth J. Norton- 1College of Agriculture, Yangtze University, Jingzhou, China
- 2School of Biological Sciences, University of Aberdeen, Aberdeen, United Kingdom
Editorial on the Research Topic
Natural Variations and Genetic Constraints on Plant Nutrition
The healthy growth, development, and productivity of plants require large numbers of essential and beneficial elements, alongside the uptake of these elements, toxic metals and metalloids are also taken up and accumulated in the tissues of plants due to the imperfect selectivity of the transporters (Clemens and Ma, 2016; Huang et al., 2020; Deng et al., 2021). A proportion of the mineral elements taken up by roots is accumulated in the edible organs for human consumption, with the exact proportion being determined by the element in question, the plant species, and the environmental conditions. The lack of essential elements leads to retarded growth of plants, as well as hidden hunger for humans (Stanton et al., 2022). It is estimated that about 2 billion people worldwide suffer from deficiencies of iron (Fe) and zinc (Zn) in their diet (Huang et al., 2020). On the other hand, excess intake of toxic elements through the food chain results in great health risk (Zhao et al., 2022). For instance, populations that consume cadmium (Cd) or arsenic (As) contaminated grains have an increased cancer risk (Deng et al., 2018; Zhao and Wang, 2020). Therefore, there is an urgency to develop ideal crops with elevated levels of Fe and Zn, as well as reduced As and Cd in their edible tissues.
Both environmental factors and genetic mechanisms contribute to elemental accumulation in plants. During the last decades, great achievements have been made in identifying the critical transporters for the accumulation of various elements (Che et al., 2018; Huang et al., 2020; Yadav et al., 2021; Stanton et al., 2022; Zhao et al., 2022). Furthermore, the increasing datasets of genome sequencing and re-sequencing, pan-genomics, and RNA-seq provide abundant data resources to study the natural variations of critical genes responsible for mineral transport and accumulation in plants (Yang et al., 2018; Norton et al., 2019; Tan et al., 2020; Ruang-areerate et al., 2021). In this special issue, Liu et al. identified 167 unique and 25 repeatable quantitative trait loci (QTLs) in rice (Oryza sativa L.) affecting the concentration of 16 elements using 3,117 single nucleotide polymorphisms (SNPs), while only 40 QTLs were detected with 175 restriction fragment length polymorphism markers with the same population (Zhang et al., 2014).
This Research Topic focuses on the genetic conservation and variation of gene alleles responsible for the accumulation and detoxification of mineral element including both essential and toxic metal (loid)s in plants. Its ultimate aim is to identify the genetic regulators of mineral accumulation in plants, so as to exploit these mechanisms to both food security and safety. Cereals represent the most significant dietary intake of both essential and toxic minerals (Li et al., 2011; Deng et al., 2019; Huang et al., 2020). In this special issue, we include four original research articles covering aspects of both essential and toxic elements accumulation in rice and wheat (Triticum aestivum L.), which are the staple food for approximately 4.0 and 2.5 billion people (Gerard et al., 2020; Huang et al., 2020), respectively.
To gain nutritionally enhanced wheat with higher levels of Fe and Zn, a population consisting of 190 recombinant inbred lines (RILs) derived from a “Kachu” × “Zinc-Shakti” cross were generated. Classical QTL analysis revealed seven new pleiotropic QTLs for grain Zn and Fe accumulation, several candidate genes including putative transporters, regulators involved in ion binding, GTP or phospholipid binding were suggested to underlie these QTLs. The identified molecular markers and RILs with elevated grain Fe and Zn levels will be valuable for breeding wheat with increased concentrations of these key nutrients for people (Rathan et al.).
Apart from the classical approach to identify QTLs, candidate gene-based association analysis, genome-wide association study (GWAS), and multivariate QTL analyses have been employed for studying genetic variation of the rice ionome in this issue (Liu et al.; Liu et al.; Pinson et al.). Several key genes involved in Zn transport and accumulation in rice have been functionally characterized, to evaluate the effects of these genes related to grain Zn content, two panels of breeding lines and a multiparent advanced generation intercross (MAGIC) population were employed. Among the examined 65 candidate genes, a total of 8 genes were detected to be significantly associated with grain Zn concentration. The contribution to the variation was estimated to range from 7.70% to 17.99%. More importantly, OsIRT1 and OsZIP7 were identified from two of the three populations, indicating their broad variations and large effects (Liu et al.). The identified superior genes and haplotypes are excellent resources for further enhancement of Zn in rice grains.
To reveal the relationships among As-related traits, multiple GWA-QTL for hull silica (Si) concentration, grain concentrations of As, phosphorus (P), sulfur (S), calcium (Ca), and copper (Cu), as well as disease severity for straighthead disorder (StHD) (putatively attributed to As toxicity), were detected with in a rice minicore collection. A total of 195 QTLs for the examined nine traits were identified, six of the 15 QTLs which were detected for grain As had not been reported previously. The accumulation of Si, P, S, and Ca were not associated with grain-As at the QTL level. Unexpectedly, only four among of the 33 QTLs for StHD co-located with QTLs for grain As, additionally StHD severity did not correlate with grain As concentration. However, StHD QTLs did overlap with Si QTLs (11/33). The results indicate that the genetic factors regulating grain As and StHD are complex, and Si accumulation in plants is most likely to contribute to StHD severity (Pinson et al.).
Covaration of mineral elements is widely observed in plant tissues and is affected by environmental factors, however, traditional QTL analyses using individual elemental concentrations as separate traits, does not take this interrelatedness into consideration. To better understand the genetic basis of covariation of multiple elements, principle component analysis (PCA) of the examined traits of a rice RIL population under various conditions was conducted and with the PCs used in the QTL analyses. As a result, 53 QTLs controlling covariance among elemental concentrations within a single environment/tissue (PC-QTLs), and 152 QTLs which determined covariation among elements across environments/tissues (aPC-QTLs) were detected. In addition to the PC-QTLs matching the QTLs detected using single traits, some PC-specific QTLs were also identified, implicating novel clues for further analysis (Liu et al.).
The genetic markers, loci, candidate genes as well as superior haplotypes responsible for variation in mineral accumulation in rice and wheat reported in this issue are potentially beneficial for generating cereals rich in essential mineral elements but with less toxic elements in grain.
Author Contributions
This manuscript was written by FD with contributions from FZ and GN.
Funding
The work in FD and FZ lab was supported by National Natural Science Foundation of China (32170276), and the Major International (Regional) Joint Research Project from NSFC-ASRT (32061143044).
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s Note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Acknowledgments
Huge thanks are given to the colleagues, authors, reviewers, and support staff who contributed to the success of this Research Topic.
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Keywords: plant nutrition, mineral elements, toxic metal(loid)s, natural variations, quantitative genetics
Citation: Deng F, Zeng F and Norton GJ (2022) Editorial: Natural Variations and Genetic Constraints on Plant Nutrition. Front. Genet. 13:941118. doi: 10.3389/fgene.2022.941118
Received: 11 May 2022; Accepted: 06 June 2022;
Published: 24 June 2022.
Edited and reviewed by:
Andrew H. Paterson, University of Georgia, United StatesCopyright © 2022 Deng, Zeng and Norton. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Fenglin Deng, dfl@yangtzeu.edu.cn