Validation and Development of COI Metabarcoding Primers for Freshwater Macroinvertebrate Bioassessment
- 1Aquatic Ecosystem Research, Faculty of Biology, University of Duisburg-Essen, Essen, Germany
- 2Centre for Water and Environmental Research, University of Duisburg-Essen, Essen, Germany
A corrigendum on
Validation and Development of COI Metabarcoding Primers for Freshwater Macroinvertebrate Bioassessment
by Elbrecht, V., and Leese, F. (2017). Front. Environ. Sci. 5:11. doi: 10.3389/fenvs.2017.00011
In the original article, the reference for Bista et al. (2017) was incorrectly written as “Carvalho, G. R., Walsh, K., Seymour, M., Hajibabaei, M., Lallias, D., Christmas, M., et al. (2017). Annual time-series analysis of aqueous eDNA reveals ecologically relevant dynamics of lake ecosystem biodiversity. Nat. Commun. 8, 1–11. doi: 10.1038/ncomms14087.” It should be “Bista, I., Carvalho, G. R., Walsh, K., Seymour, M., Hajibabaei, M., Lallias, D., et al. (2017). Annual time-series analysis of aqueous eDNA reveals ecologically relevant dynamics of lake ecosystem biodiversity. Nat. Commun. 8, 1–11. doi: 10.1038/ncomms14087.”
In the original article, there was a mistake in Table 2 as published. “Ephemeroptera” and taxa abundance (8) are missing in the table. The corrected Table appears below. The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way.
Table 2. Number of species recovered with the newly developed primers and data on 16S and Folmer primers from previous tests (Elbrecht and Leese, 2015; Elbrecht et al., 2016).
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
References
Elbrecht, V., and Leese, F. (2015). Can DNA-based ecosystem assessments quantify species abundance? Testing primer bias and biomass—sequence relationships with an innovative metabarcoding protocol. PLoS ONE 10:e0130324. doi: 10.1371/journal.pone.0130324
Keywords: DNA barcoding, primer development, primer evaluation, primer bias, ecosystem assessment, in silico PCR, invertebrates
Citation: Elbrecht V and Leese F (2017) Corrigendum: Validation and Development of COI Metabarcoding Primers for Freshwater Macroinvertebrate Bioassessment. Front. Environ. Sci. 5:38. doi: 10.3389/fenvs.2017.00038
Received: 04 May 2017; Accepted: 13 June 2017;
Published: 23 June 2017.
Edited and reviewed by: Michael M. Douglas, University of Western Australia, Australia
Copyright © 2017 Elbrecht and Leese. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Vasco Elbrecht, VmFzY28uRWxicmVjaHRAdW5pLWR1ZS5kZQ==