Biljana Musicki
Arthur L. Burnett- Department of Urology, The James Buchanan Brady Urological Institute, The Johns Hopkins School of Medicine, Baltimore, MD, United States
Hypogonadism is common in men with sickle cell disease (SCD) with prevalence rates as high as 25%. Testicular failure (primary hypogonadism) is established as the principal cause for this hormonal abnormality, although secondary hypogonadism and compensated hypogonadism have also been observed. The underlying mechanism for primary hypogonadism was elucidated in a mouse model of SCD, and involves increased NADPH oxidase-derived oxidative stress in the testis, which reduces protein expression of a steroidogenic acute regulatory protein and cholesterol transport to the mitochondria in Leydig cells. In all men including those with SCD, hypogonadism affects physical growth and development, cognition and mental health, sexual function, as well as fertility. However, it is not understood whether declines in physical, psychological, and social domains of health in SCD patients are related to low testosterone, or are consequences of other abnormalities of SCD. Priapism is one of only a few complications of SCD that has been studied in the context of hypogonadism. In this pathologic condition of prolonged penile erection in the absence of sexual excitement or stimulation, hypogonadism exacerbates already impaired endothelial nitric oxide synthase/cGMP/phosphodiesterase-5 molecular signaling in the penis. While exogenous testosterone alleviates priapism, it disadvantageously decreases intratesticular testosterone production. In contrast to treatment with exogenous testosterone, a novel approach is to target the mechanisms of testosterone deficiency in the SCD testis to drive endogenous testosterone production, which potentially decreases further oxidative stress and damage in the testis, and preserves sperm quality. Stimulation of translocator protein within the transduceosome of the testis of SCD mice reverses both hypogonadism and priapism, without affecting intratesticular testosterone production and consequently fertility. Ongoing research is needed to define and develop therapies that restore endogenous testosterone production in a physiologic, mechanism-specific fashion without affecting fertility in SCD men.
1 Introduction
Sickle cell disease (SCD) is the most common hereditary hematologic disorder in the United States, which affects an estimated 100,000 Americans, mostly African-Americans, and millions of people globally (1). Patients with SCD experience acute complications, such as painful vaso-occlusive episodes, and chronic multi-organ damage, which heighten their risks for morbidity and mortality (2). SCD was long considered to be a disease of children and young adults because of its devastating natural progression. Due mostly to universal newborn screening and early therapeutic intervention, life expectancy in patients with SCD has steadily improved over the last 30 years, and recent studies have estimated the median survival for patients with SCD at 60 years (3). Extended survival outcomes have, however, led to an increase in long-term complications of this disease.
SCD is associated with hypogonadism (total testosterone levels below 300 ng/dl), which develops in up to 25% of men with this disease (4). This rate contrasts with the 6-12% prevalence rate of symptomatic hypogonadism in otherwise healthy middle aged and older men, who manifest an age-related decline in testosterone production (5). The impact of testosterone deficiency in the SCD male population is evident, based on its symptomatic effects, e.g., impaired physical and sexual maturation, reduced libido, erectile dysfunction, decreased physical strength, fatiguability, mood changes, and infertility (6, 7). Attempts to address this problem are, however, hampered by limited understanding of the mechanism of hypogonadism in SCD.
This review focuses on the mechanism of testosterone deficiency in SCD, the impact of hypogonadism on health- and reproduction-related issues in SCD males, and novel strategies to drive endogenous testosterone biosynthesis. These strategies may translate into clinical therapeutic opportunities for preserving sexual function and fertility, and possibly other conditions, adversely affected by hypogonadism in SCD.
2 Sickle Cell Disease
SCD is caused by a single point mutation in the β-globin gene of hemoglobin, leading to the expression of abnormal sickle hemoglobin (HbS). Traditionally, the pathophysiology of SCD was thought to result exclusively from the polymerization of HbS under hypoxic conditions, causing erythrocytes to become deformed, sludge, and occlude blood vessels, along with oxidative stress, inflammation, and hemolytic anemia (8). More recent studies show that SCD is also characterized by a chronic deficiency of the endogenous vasodilator nitric oxide (NO) and vascular dysfunction (8, 9). As a consequence, SCD leads to progressive multi-organ failure resulting in pulmonary hypertension, leg ulcers, renal failure, stroke, infarct, retinopathy, neurocognitive impairment, bone loss, and priapism (2, 9, 10).
2.1 Hypogonadism in Sickle Cell Disease
Clinical research has documented a high frequency of testosterone deficiency in SCD, with prevalence rates as high as 25% (4). In a small number of clinical studies investigating hypogonadism in SCD, findings regarding its etiology and clinical implications have varied. Studies have reported elevated luteinizing hormone (LH) and follicle-stimulating hormone (FSH) levels in patients with SCD (primary hypogonadism; 6, 11–14). Repeated testicular infarction is observed in some men with SCD, attributed to erythrocyte sickling, obstructed blood flow, and hypoxia (15), and this course has been proposed to be a contributing factor for testicular failure (16–19). In contrast, studies report decreased LH and FSH in patients with SCD (secondary hypogonadism; 4, 20, 21). Furthermore, compensated hypogonadism (characterized by increased gonadotropins and normal testosterone levels) has also been identified in men with SCD (22). Smaller testis size in SCD men (6, 23) and reduced testis weight in SCD mice (24) is further evidence of hypogonadism related to this disease.
In recent years, progress has been made toward understanding the mechanism of testosterone deficiency in SCD, and primary hypogonadism has now been established as the principal cause for this hormonal abnormality. Oxidative/nitrosative stress is implicated in defective testosterone production by affecting the expression or enzymatic activation of several steroidogenic enzymes, or by depletion of antioxidants (25–27). In the vasculature of humans and experimental animals with SCD, reactive oxygen species (ROS)-generated enzymes NADPH oxidase (NOX) and xanthine oxidase, endothelial NO synthase (eNOS) uncoupling, autooxidation of HbS, heme iron release, and increased asymmetric dimethylarginine have been described (28, 29). Diverse stimuli associated with these redox sources include hypoxia, angiotensin II, proinflammatory cytokines, vasoconstrictors, growth factors, metabolic factors, and superoxide itself (30).
The testis of the SCD mouse exhibits upregulation of 4-hydroxy-2-nonenal (4-HNE), a major end product of lipid peroxidation, upregulation of NOX gp91phox subunit, and uncompensated expression of the antioxidant enzyme glutathione peroxidase-1, all consistent with a heightened and uncontrolled redox environment in the SCD mouse Leydig cell (31). Increased NOX-derived oxidative stress reduces protein expression of steroidogenic acute regulatory protein (StAR) (but not cholesterol side-chain cleavage enzyme) in Leydig cells of the SCD mouse testis, which initiates cholesterol transfer into mitochondria. Reduced transport of cholesterol to mitochondria of Leydig cells in the SCD testis accounts for primary hypogonadism (31).
Secondary hypogonadism appears to represent patients having more severe or progressive forms of SCD, who exhibit more frequent abnormalities of LH and FSH in comparison with patients having mild disease (20). While not completely understood, secondary hypogonadism may be the result of vasoocclusion of hypothalamic-pituitary small blood vessels, or pituitary infarction (11).
2.1.1 Hypogonadism, Reproductive Issues, and Health-Related Quality of Life in SCD
Testosterone plays a critical role in muscle physiology, body development, bone density, sexual function, fertility, as well as social, emotional, and neurocognitive functioning in males (32). Patients with SCD exhibit reduced height and weight, decreased physical strength, and delayed sexual maturation (23). Low levels of testosterone have been associated with very low bone mass density in SCD patients compared with those having normal bone mass density (33). Psychological distress, such as mood changes, increased anxiety, extreme fatigue, social withdrawal, and depression, and neurocognitive impairment, such as impaired executive function, attention, and processing speed, are well recognized complications of SCD (34–36). However, it is not understood whether declines in physical, psychological, and social domains of health in SCD patients are related to low testosterone levels or are consequences of other abnormalities of SCD. Future studies are warranted to evaluate this possible consequence of hypogonadism in SCD.
Although poorly studied in SCD, male infertility is recognized to be a common complication of this disease (23, 37–39). Impaired male fertility in SCD is due to multiple causes, including hypogonadism, gonadal failure and sperm abnormalities (such as oligospermia, reduced sperm motility and density, and abnormal sperm morphology), decreased ejaculate volume, and delayed or impaired sexual development. Prevalence rate of at least one abnormal sperm parameter in male patients with SCD is 91% (40). Erectile dysfunction, largely as a result of penile damage from recurrent or prolonged priapism, further contributes to reduced fertility in SCD men (23).
2.1.2 Hypogonadism and SCD-Related Priapism
Priapism is a pathologic condition of prolonged penile erection in the absence of sexual excitement or stimulation (41). Ischemic priapism, which features little or absent intracorporal blood flow resulting in painful erections, is prevalent in men with SCD, occurring in as many as 48% of men, with a mean age of onset of 15 years (42, 43). Repeated episodes of priapism may lead to irreversible damage to erectile tissue and permanent erectile dysfunction (42, 44, 45) and cause psychological distress, impaired sexual relationships, and reduced quality and function of life (46). The prevalence rate of erectile dysfunction associated with recurrent ischemic priapism in SCD patients is as high as 47.5% (47).
The historical premise is that androgens are causative in the pathophysiology of priapism. However, this notion is now challenged. Reports of no increase in priapism in testosterone deficient men administered testosterone gel at eugonadal levels (48), as well as reduced priapism occurrences in testosterone deficient men with SCD receiving long-acting testosterone undecanoate injections (49) oppose earlier conceptions that testosterone therapies cause priapism. It is now established that physiologic testosterone administration does not cause priapism and, in contrast, this intervention promotes molecular mechanisms that favor normal erection responses. In fact, priapism in SCD is associated with decreased testosterone levels. A potential role for testosterone in correcting priapism acknowledges that androgens contribute to physiologic erectile tissue responses. Testosterone and dihydrotestosterone promote physiologic relaxation of penile arteries and cavernous tissue, and androgen deficiency decreases the expression and enzymatic activities of eNOS, neuronal NOS, and phosphodiesterase type 5 (PDE5) in the penis, the main players in penile erection (50).
The mechanisms by which testosterone deficiency contributes to priapism has recently been elucidated. In a mouse model of SCD, characterized by both primary hypogonadism and priapism (51), testosterone replacement at eugonadal levels corrects priapism. At the molecular level, normalized testosterone levels reverse downregulated eNOS activity via a nongenomic mechanism by normalizing downregulated P-Akt (Ser-473) and P-eNOS (Ser-1177) protein expressions in the penis (51). Increased NO reverses downregulated protein expression and activity of PDE5, the enzyme which degrades cGMP in the penis (52–56). Testosterone’s effect on PDE5 protein expression is believed to be mediated by increased NO-induced accumulation of cGMP, which binds to cGMP response sequences in the PDE5 promoter (57). Testosterone’s effect on PDE5 catalytic activity is due to phosphorylation of PDE5 on Ser-92 by cGMP-mediated activation of protein kinase G, which stimulates binding of cGMP to the regulatory domain of PDE5 (58). Upregulated PDE5 protein expression and activity in the penis restores the mechanism for cGMP degradation, thereby preventing excessive accumulation of this nucleotide upon neurostimulation. By controlling the amount of cGMP, which causes relaxation of smooth muscles in the penis and penile erection, priapic activity is lessened (51). This proof-of-principle study supports testosterone deficiency as a cause for SCD-associated priapism by exacerbating already impaired NO molecular signaling in the penis.
In contrast to its physiologic doses, testosterone at supraphysiologic doses decreases NO production from eNOS and increases oxidative stress in endothelial cells (59–61). This may partially explain findings described in several case reports in men that, at excessive dosing, testosterone may trigger priapism rather than reduce it (62–64).
Priapism is one of very few complications of SCD that has been studied in the context of hypogonadism. It is interesting to observe that low testosterone exhibits opposing erection phenomena in the general population of men vs men with SCD: while low testosterone may contribute to decreased erection in the general population having cardiovascular or metabolic factors affecting erectile tissue function, it results in uncontrolled erection in the SCD population, which has a severely disturbed PDE5 regulatory pathway in the penis. However, it is noted that achieving physiologic “eugonadal” effects in the penis is healthful in both populations.
3 Testosterone Replacement Strategies
Traditional approaches for managing testosterone deficiency in general have largely centered on exogenous administration of testosterone. Testosterone therapies and their relative usages are: transdermal testosterone gel therapy (70%), testosterone injections (17%), transdermal testosterone patches (10%), and other forms of testosterone therapy, such as an oral formulation (3%) (65, 66). However, limitations exist with these current therapies. Adverse side effects are commonly described in association with exogenous testosterone administration, including supraphysiologic levels of testosterone, local irritation with applications, gynecomastia, erythrocystosis, hepatotoxicity, and sleep apnea (67). Adverse prostate health risks of benign prostate enlargement and prostate cancer as well as cardiovascular risks (i.e., edema, heart attack, stroke) have also been contended to be potential risks of testosterone therapy (68). Impaired sperm production and infertility are also documented risks of exogenous testosterone therapies, by virtue of feedback inhibition of central gonadotropin release. Such therapies suppress LH, which in turn suppress Leydig cell-stimulated testosterone production, resulting in reduced intratesticular testosterone concentrations needed for spermatogenesis (67, 69). Because of the contraceptive effect exerted by exogenous testosterone preparations, many young men with hypogonadism desiring to retain reproductive function are precluded from pursuing exogenous testosterone therapies as a therapeutic option.
Alternatives to exogenous testosterone treatment have been explored, with the main objective to drive endogenous testosterone production and in turn preserve fertility. Current options include selective estrogen receptor modulators (SERMs), aromatase inhibitors, and human chorionic gonadotropin (hCG) (70). Both SERMs (e.g., clomiphene citrate and tamoxifen citrate), which serve as estrogen receptor antagonists, and aromatase inhibitors (e.g., letrozole, anastrozole, and testolactone), which block the conversion of testosterone to estradiol, result in decreased estrogen feedback to the hypothalamus thereby effecting a natural increase in gonadotropin release (70). Their efficacy in increasing testosterone production is limited in men with normal or elevated LH levels who manifest a testosterone production defect at the testicular level. hCG, operating as an LH analogue, serves to stimulate Leydig cell production of testosterone. Its efficacy is limited in men whose Leydig cells are not functionally responsive to LH because of decreased receptor function or capacity for testosterone production (65, 71).
These reports indicate that currently available testosterone therapeutic options aiming to enhance endogenous testosterone production fall short in addressing testosterone deficiency associated with testicular failure. This shortcoming is relevant generally and for hypogonadal males with SCD. Specifically in males with SCD, exogenous testosterone would further affect fertility by decreasing intratesticular testosterone production needed for spermatogenesis.
4 Endogenous Mechanism-Specific Molecular Targets for Testosterone Production
Targeting mechanism-specific endogenous sources of testosterone production in the SCD testis to produce eugonadal levels of the hormone directly addresses primary hypogonadism. As transfer of cholesterol from the outer to the inner mitochondrial membrane of Leydig cells in the testis is the principal site of regulation of steroid hormone biosynthesis, and is impaired in SCD, targets for stimulating testosterone production may involve transduceosome protein components. The transduceosome is an ensemble of mitochondrial and cytosolic proteins responsible for cholesterol translocation from intracellular stores to the inner mitochondrial membrane (72). Translocator protein (TSPO) is a high-affinity drug- and cholesterol-binding mitochondrial protein, and its protein expression is decreased in the testis of SCD mice (73, 74). The TSPO-dependent import of StAR into mitochondria and the association of TSPO with the outer/inner mitochondrial membrane contact sites drives intramitochondrial cholesterol transfer and subsequent steroid formation (73). Previous studies have shown that TSPO drug ligands activate steroid production by MA-10 mouse Leydig tumor cells and by mitochondria isolated from other steroidogenic cells (75–77). Furthermore, pharmacologic stimulation of TSPO stimulates testosterone production, both in vitro by Leydig cells isolated from aged rats and in vivo in aged rats, without reducing intratesticular testosterone concentrations or sperm number (78, 79). These studies oppose several previous reports which questioned the role and extent of involvement of TSPO in mitochondrial cholesterol import and steroidogenesis (80, 81).
A recent study in a SCD mouse model demonstrated that pharmacologic stimulation of TSPO corrects priapism. Treatment of SCD mice with TSPO-selective drug ligand N,N-dihexyl-2-(4-fluorophenyl) indole-3-acetamide (FGIN-1-27) produces eugonadal levels of testosterone. Normalized testosterone levels corrects priapism without decreasing intratesticular testosterone production (74). At the molecular level, TSPO ligand, by normalizing testosterone levels, restores PDE5 activity and decreases NOX-mediated increase in oxidative stress in the penis. Conceivably, this effect of testosterone pertains to recovered control of NO/cGMP responsiveness associated with restored PDE5 function. The mechanism underlying testosterone’s inhibitory effect on NOX expression and activity is not known, but may be indirect through the improvement of endothelial function. In human endothelial cells and mouse aorta, NO S-nitrosylates and inhibits p47phox subunit of NOX, inhibits protein expression of gp91phox and p47phox subunits of NOX, and inhibits superoxide production (82–84). These findings suggest that targeting endogenous testosterone production in the SCD testis by pharmacologic activation of protein components involved in cholesterol transport could be a novel, targetable pathway to correct primary hypogonadism and ameliorate testosterone deficiency-associated health conditions without affecting fertility.
While not examined, it is plausible that, in addition to TSPO, other cytosolic or outer mitochondrial membrane protein components involved in cholesterol transport from intracellular stores to the inner mitochondrial membrane (such as voltage dependent anion channel 1, negative protein adaptor 14-3-3ϵ, or AAA domain-containing protein 3A) (72), may be targeted in the SCD testis to increase endogenous testosterone production. Because pharmacologic activation of TSPO is independent of LH, it is conceivable that this approach may treat secondary hypogonadism, or mixed primary and secondary hypogonadism, as well. Other possible mechanism-based targets in the SCD testis include increased oxidative stress, or enzymatic sources of oxidative stress (such as NOX), which are enhanced in SCD-associated primary hypogonadism (Figure 1).
Figure 1 A model depicting mechanism-specific endogenous targets within the Leydig cells of the testis which can be modulated to reverse hypogonadism in SCD. Targets include: inhibition of enzymatic sources of oxidative stress, such as NOX; inhibition of increased oxidative stress, which decreases protein synthesis of enzymes involved in cholesterol transport to the mitochondria, such as StAR and TSPO; stimulation of cytosolic or outer mitochondrial membrane protein components involved in cholesterol transport from intracellular stores to the inner mitochondrial membrane; ROS, reactive oxygen species; StAR, steroidogenic acute regulatory protein; TSPO, translocator protein.
Of note, L-glutamine, one of the 3 recently FDA-approved treatments for SCD (L-glutamine, crizanlizumab, and voxelotor), increases glutathione-dependent anti-oxidation in the testis and testosterone levels, at least in sleep-deprived rats (85), while alleviating primary hypogonadism and protecting erythrocytes against oxidative damage.
5 Discussion
SCD affects millions of people throughout the world, mostly of African ancestry, and is recognized by the World Health Organization and United Nations as a global health issue. In the United States, health outcomes for people with SCD have improved in the past few decades. Despite medical advances, life expectancy for individuals with SCD in the United States remains 20 to 30 years lower than that of the average American. It has been recognized that research and treatment efforts for SCD lag behind that of other chronic genetic illnesses, such as hemophilia and cystic fibrosis, requiring legislative attention (86, 87). In correlation, less FDA-approved therapies are currently available for SCD. The Sickle Cell Disease Comprehensive Care Act, signed into law in December 2018, represents a commitment by the government to continue research towards increasing the understanding of prevalence, distribution, outcomes, and therapies associated with SCD.
Amidst health care disparities among ethnic populations in the United States, limited knowledge and action surround hypogonadism in SCD, in spite of its long-term and costly health problems. While many studies have evaluated the mechanism and health-related issues of hypogonadism in the general adolescent population, very few studies have focused on hypogonadism in the SCD population. For example, although an estimated 1 in 4 SCD patients exhibits low testosterone levels, no studies have assessed the testosterone-dependent health-related quality of life profiles of SCD patients.
Despite inequity in federal and foundation research funding, basic scientific advances and potential new directions to target testosterone deficiency in SCD are being made in recent years. The objective of finding and targeting mechanism-specific endogenous sources of testosterone production appears necessary for preserving sexual function and fertility in the SCD young adult population, particularly in light of the harms of exogenous testosterone therapies.
Author Contributions
The authors confirm contribution to the manuscript as follows: BM and AB critically reviewed the literature. BM drafted the article. BM and AB reviewed and revised the manuscript. BM and AB contributed to the article and approved the submitted version.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s Note
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Keywords: nitric oxide, testosterone replacement, TSPO, PDE5, erectile dysfunction, infertility, oxidative stress
Citation: Musicki B and Burnett AL (2022) Testosterone Deficiency in Sickle Cell Disease: Recognition and Remediation. Front. Endocrinol. 13:892184. doi: 10.3389/fendo.2022.892184
Received: 08 March 2022; Accepted: 31 March 2022;
Published: 03 May 2022.
Edited by:
Vassilios Papadopoulos, University of Southern California, United StatesReviewed by:
Carol Podlasek, University of Illinois at Chicago, United StatesPeter Liu, The Lundquist Institute, United States
Copyright © 2022 Musicki and Burnett. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Biljana Musicki, Ym11c2lja2lAamhtaS5lZHU=