Corrigendum: Adeno-Associated Virus-Mediated Gain-of-Function mPCSK9 Expression in the Mouse Induces Hypercholesterolemia, Monocytosis, Neutrophilia, and a Hypercoagulative State

A Corrigendum on
Adeno-Associated Virus-Mediated Gain-of-Function mPCSK9 Expression in the Mouse Induces Hypercholesterolemia, Monocytosis, Neutrophilia, and a Hypercoagulative State

by Louloudis, G., Ambrosini, S., Paneni, F., Camici, G. G., Benke, D., and Klohs, J. (2021). Front Cardiovasc Med. 8:718741. doi: 10.3389/fcvm.2021.718741

In the original article, there was a mistake in Figure 1H as published. The graph incorrectly depicting higher serum HDL-cholesterol levels in control AAV mice on standard diet compared to the rest of the experimental groups. The corrected Figure 1H appears below. It depicts higher serum HDL-cholesterol levels in the mPCSK9-AAV mice on HFD compared to the rest of our experimental groups.

A correction has been made to Results, Elevated PCSK9 plasma levels and hypercholesterolemia induced by mPCSK9-AAV expression and intake of Western-type HFD, paragraph 2:

“Furthermore, mPCSK9-AAV injected mice on the HFD had elevated total, HDL-cholesterol and non-HDL cholesterol serum levels (Figures 1F–H).”

FIGURE 1

Figure 1. Verification of the mPCSK9-AAV mouse model of hypercholesterolemia. (A) Body weights of the four experimental groups: Control AAV + Standard diet (black circles, n = 12), Control AAV + HFD (black squares, n = 10), mPCSK9-AAV + Standard diet (gray upward triangles, n = 11), and mPCSK9-AAV + HFD (gray downward triangles, n = 10). Mean ± SD; repeated-measures ANOVA with Holm–Sidak post-hoc test;^**p ≤ 0.01, ^****p ≤ 0.0001. (B) Mouse PCSK9 plasma concentration in Control AAV + Standard diet (circles, n = 7), Control AAV + HFD (squares, n = 6), mPCSK9-AAV + Standard diet (upward triangles, n = 7), and mPCSK9-AAV + HFD (downward triangles, n = 7) groups. Data are the mean of three independent replicates. Mean ± SD; two-way ANOVA with Tukey's post-hoc test; ^****p ≤ 0.0001. (C,D) Hepatic Ldl receptor (Ldlr) levels in control-injected (n = 6) and mPCSK9-AAV-injected mice (n = 6) on standard diet normalized to total protein. Data are the mean of three independent replicates. Two gels were loaded per replicate. Mean ± SD; unpaired t-test; ^****p ≤ 0.0001. (E) Representative images of Oil-red O-stained thoracic aortae of mPCSK9-AAV and control AAV mice on HFD. Mice did not develop atherosclerotic lesions in the selected time frame. Scale bar, 2 mm. (F–H) Serum total cholesterol (F), non-HDL cholesterol (G), and HDL cholesterol (H) in the different groups: Control AAV + Standard diet (n = 6), Control AAV + HFD (n ≥ 8), mPCSK9-AAV + Standard diet (n ≥ 9), and mPCSK9-AAV + HFD (n ≥ 7). Mean ± SD; two-way ANOVA with Tukey's post-hoc test; ^*p ≤ 0.05, ^**p ≤ 0.01, ^***p ≤ 0.001, ^****p ≤ 0.0001. AAV, adeno-associated virus; HFD, high-fat diet; HDL, high-density lipoprotein.

Furthermore, a correction has been made to the Discussion, paragraph 3:

“Concomitantly, the non-HDL-cholesterol and HDL-cholesterol concentrations were significantly higher in mPCSK9-AAV mice on HFD compared to control AAV mice on standard diet (Figures 1G,H).”

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

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