Meseret N. Teferra
David A. Hobbs
Robyn A. Clark3- 1Medical Device Research Institute, College of Science and Engineering, Flinders University, Adelaide, SA, Australia
- 2Allied Health & Human Performance, University of South Australia, Adelaide, SA, Australia
- 3College of Nursing and Health Science, Flinders University, Adelaide, SA, Australia
Background: With cardiovascular disease continuing to be the leading cause of death and the primary reason for hospitalization worldwide, there is an increased burden on healthcare facilities. Electronic-textile (e-textile)-based cardiac monitoring offers a viable option to allow cardiac rehabilitation programs to be conducted outside of the hospital.
Objectives: This study aimed to determine whether signals produced by an e-textile ECG monitor with textile electrodes in an EASI configuration are of sufficient quality to be used for cardiac monitoring. Specific objectives were to investigate the effect of the textile electrode characteristics, placement, and condition on signal quality, and finally to compare results to a reference ECG obtained from a current clinical standard the Holter monitor.
Methods: ECGs during different body movements (yawning, deep-breathing, coughing, sideways, and up movement) and activities of daily living (sitting, sitting/standing from a chair, and climbing stairs) were collected from a baseline standard of normal healthy adult male using a novel e-textile ECG and a reference Holter monitor. Each movement or activity was recorded for 5 min with 2-min intervals between each recording. Three different textile area electrodes (40, 60, and 70 mm2) and electrode thicknesses (3, 5, and 10 mm) were considered in the experiment. The effect of electrode placement within the EASI configuration was also studied. Different signal quality parameters, including signal to noise ratio, approximate entropy, baseline power signal quality index, and QRS duration and QT intervals, were used to evaluate the accuracy and reliability of the textile-based ECG monitor.
Results: The overall signal quality from the 70 mm2 textile electrodes was higher compared to the smaller area electrodes. Results showed that the ECGs from 3 and 5 mm textile electrodes showed good quality. Regarding location, placing the “A” and “I” electrodes on the left and right anterior axillary points, respectively, showed higher signal quality compared to the standard EASI electrode placement. Wet textile electrodes showed better signal quality compared to their dry counterparts. When compared to the traditional Holter monitor, there was no significant difference in signal quality, which indicated textile monitoring was as good as current clinical standards (non-inferior).
Conclusion: The e-textile EASI ECG monitor could be a viable option for real-time monitoring of cardiac activities. A clinical trial in a larger sample is recommended to validate the results in a clinical population.
Introduction
Cardiovascular Disease (CVD) is the number one non-communicable disease and the highest cause of death worldwide, with an estimated life loss of 17.92 million people in 2015 (1). This number is predicted to rise to 23.6 million by 2030 (2). Heart attack and stroke are the most common events constituting more than 85% of the CVD incidents in 2017 (1, 3).
Population aging in developed countries increases the demand for available health care. At the same time, the prevalence of CVD is also higher in this older age group, placing increased pressure on the medical system (4). A study published in 2019 reported that long-term ambulatory ECG monitoring could play a vital role in detecting the onset of ventricular dysrhythmias and atrial fibrillation (5). Ventricular dysrhythmias are the prominent factors indicating heart failure, stroke, and cardiac death. Electronic-textile-based cardiac monitoring offers a viable option (6) for long-term ambulatory monitoring outside of the hospital premises.
Electronic textiles, also known as e-textiles, are defined as “fabrics that have electronics and interconnections woven into them (7).” In the field of cardiology, researchers have developed e-textile sensors that can monitor the cardiac activities of patients while they are engaging in their day-to-day life (8) or in hospital settings (9). These e-textile ECG electrodes produce signals of acceptable quality (10) and are resistant to repeated washing in aqueous solutions without losing their properties (11, 12). However, an e-textile ECG monitor with a diagnostics capability is yet to be reported (8).
The 12-lead ECG has superior dependability and is considered the gold standard for diagnostic ECG (13). However, the conventional Holter monitor has ECG lead wires and 10 sticky electrodes, making it less comfortable for extended ambulatory monitoring. A wireless ECG monitor based on an EASI electrode configuration (14) was implemented to address this issue. This ECG monitor has a reduced number of leads (three base ECG leads; VAI, VES, and VAS) and only five electrodes to realize the equivalence of a 12-lead ambulatory cardiac monitor.
Objectives
This research focused on the testing and evaluation of ECG signals during activities of daily living with an e-textile ECG monitor with textile electrodes in an EASI configuration. The objectives of the experiments were to:
1. Examine the effect of the textile electrode characteristics (area and thickness) on signal quality
2. Investigate the effect of electrode placement on signal quality
3. Examine the effect of electrode condition due to sweating on signal quality; and
4. Compare the performance of the e-textile ECG monitor to that of a traditional Holter monitor.
Materials and Methods
The E-Textile ECG Monitor
An ECG monitor consisting of a smart ECG vest, textile electrodes, and miniature ECG hardware with a Java-based real-time ECG viewer and data logger was designed. The ECG hardware weighs 152 g and has a built-in Bluetooth module that can transmit data up to a maximum distance of 100 m. The ECG monitor measures ECG using textile sensors and wiring embedded within a garment. The ECG vest has a lining covering made of a modified commercial t-shirt from K-mart (a local department store). The smart ECG vest is shown in Figure 1.
Figure 1. The e-textile ECG vest with textile electrodes and embedded wiring (right) the EASI electrodes attachment site on the smart ECG vest (RLD—the reference Right Leg Drive electrode) (left).
Electrodes are placed in an EASI configuration according to Feild et al. (14): (i) electrode “E” on the lower sternum at the fifth intercostal space; (ii) electrode “A” on the same level as the “E” electrode on the left mid-axillary line; (iii) electrode “I” on the same level as the “E” electrode on the right mid-axillary line; (iv), and electrode “S” at the top of the sternum, on the manubrium.
Data Collection Protocol
The experiment was divided into two phases. During the first phase, ECGs during daily living activities (yawning, coughing, deep breathing, sitting/standing from a chair, lying on a bed in a supine position, making a call using a mobile phone, and climbing stairs) were collected from an e-textile ECG monitor to address Research Objectives 1–3. In the latter phase, the identical setups in the first stage were used to acquire ECG simultaneously from the proposed e-textile ECG monitor and a reference standard 3-leads Holter monitor (SEER light ambulatory ECG from General Electric) to answer Research Objective.
Each movement or activity was recorded for 5 min, with 2-min intervals between each recording. After each session, the lining covering of the ECG vest and the textile electrodes were replaced before the new test was conducted.
Participants
Ethics approval to collect ECG based on the data collection protocol outlined in section Data Collection Protocol from healthy adult participants was obtained from the Flinders University Social and Behavioral Research Ethics Committee (SBREC: project code – 8490). Due to the outbreak of COVID-19 pandemic, it was not possible to collect data from members of the public. However, the protocol was adapted to a COVID safe version, and data were collected from a single healthy male volunteer (age 34, BMI 22.5 kg/m2).
Signal Quality Index (SQI) Parameters
The following parameters were defined to evaluate the accuracy and reliability of the e-textile ECG monitor.
Signal to Noise Ratio (SNR)
Signal to Noise Ratio (SNR) measures the relative power between the desired signal and unwanted interference. SNR is one of the parameters used extensively in signal processing (15–17). SNR is defined in (1):
The equations should be inserted in editable format from the equation editor.
Where x(i) – clean / filtered ECG signal and xr(i) – the raw ECG signal.
The higher the SNR value, the better the energy content in the e-textile ECG. For example, a lower SNR requires complex signal processing algorithms to reduce and remove noise (18).
Approximate Entropy
Approximate entropy (ApEn) is a statistical method used to determine the dynamic nature (randomness) of a noisy time-series signal (19). Given the variable nature of the ECG signal, the ApEn (2) of the collected data was used to study irregularities in the acquired ECG (20)1:
Where: Cm(r) – the prevalence of repetitive patterns of length m in SN; SN – sequence of length N; m – pattern length and r – similarity criteria.
The ApEn is interpreted differently in different disciplines. For example, lower ApEn in heart rate variability (HRV) analysis might indicate underlying pathology. However, the ApEn in the context of this study refers to the complexity and randomness of the acquired ECG, where a higher ApEn value signifies increased noise (21). The ApEn analysis was conducted for a minimum of 1,000 data points based on a similarity criterion of 0.2 and a pattern length of 2, as recommended by Pincus and Goldberger (21).
The Baseline Power Signal Quality Index
The baseline power signal quality index (basSQI) (3) is used to examine ECG noise artifact in the low-frequency region (f ≤ 1 Hz) as a result of deep breathing, coughing, yawning, and various body movements (16). The higher the value of basSQI, the better the signal quality. Clifford et al. (22) showed that a good quality signal had a basSQI value of 0.996 while a poor-quality ECG signal scored a basSQI value of 0.5. Therefore, a basSQI ≥ 0.95 was considered the minimum acceptable baseline low-frequency noise in this study.
QRS Duration and QTc Intervals
The clinical importance of the QRS duration (23, 24) and QT intervals (25, 26) to diagnose and predict possible cardiac abnormalities are well-established concepts. For an ECG monitor to have a diagnostic application, it should be able to acquire a signal with QRS duration and QT intervals equivalent to the standard 12-lead ECG (27). The QTC was calculated based on the following formula (4) (27):
Where: RR is the time between two consecutive R peaks on the ECG tracing.
In the study, the QRS duration (normal QRS, NQRS: 0.08 – 0.12 s; long QRS, LQRS: > 0.12 s) and the corrected QT interval (QTC; short QT: <0.36 s; long QT: > 0.45 s) were used to evaluate the performance of the textile ECG monitor against the reference Holter monitor. If the values between the two systems were different, this was assumed to be a result of noise, so signals were then filtered using a MATLAB-based 2nd order high pass Butterworth filter (fc = 0.67 Hz) to remove the noise, and the results were again compared.
Three peak detection algorithms [Pan and Tompkins, State-Machine, and Multilevel Teager Energy Operator (MTEO)] from BioSigKit (28), a MATLAB toolkit for Bio-Signal analysis, were used to detect the Q, R, S, and T waves of the acquired ECG.
Electrode Characteristics
The relation between the noise introduced during a series of controlled movements and activities of daily living and the textile electrodes surface area was studied. Custom textile electrodes (Figure 2) of different contact surface areas (40, 60, and 70 mm2) were produced in the Medical Device Research Institute laboratory at Flinders University, South Australia. These were constructed from squares of silver-plated nylon conductive fabric (Adafruit Industries, New York, U.S.) sewn onto 3 mm thick Statfree® conductive polyurethane foam. Then, ECGs were acquired from these textile electrodes to investigate the effect of electrode surface area on signal quality. The thickness of the electrodes was kept at 3 mm throughout this study.
Figure 2. The e-textile ECG electrodes; left—front view (user side), middle—back view with male snap fastner (attached to the ECG vest) and right—textile electrode attachment female snap fastner on the ECG vest.
In a second experiment, the effect of electrode thickness (padding of the textile electrodes) on ECG signal transduction was assessed. 60 mm2 squares of silver-plated nylon conductive fabric were sewn onto 3, 5, and 10 mm thick Statfree® conductive polyurethane foam to produce padded textile-electrodes of 3, 5, and 10 mm thickness. ECGs were collected with the standard EASI electrode configuration throughout the experiment.
Electrode Placement
Optimal electrode placement remains an active area of research in cardiac monitoring (8, 29). As the textile electrodes are not attached to the skin firmly, it is possible for the textile electrodes to move during certain activities. It was, therefore, necessary to study the effect of electrode position on signal quality. The lateral electrodes (A and I) were more likely to be knocked or moved during activity. Hence, we wanted to see the effect of varying the electrode position. Therefore, in the experiment, the positions of the two textile sensors at “A” and “I” were varied into three positions—anterior axillary, mid-axillary, and posterior-axillary and at the level of the “E”-electrode while keeping the “E” and “S” electrodes at their defined positions. Throughout the experiment, 70 mm2 textile electrodes of 3 mm thickness were used.
Electrode Condition
An experiment was conducted to investigate the effect of moisture from sweating on signal quality. Signals from “wet” and “dry” electrodes were also compared to those from standard commercial Ag/AgCl electrodes.
An initial ECG was obtained with the participant in a relaxed seated position. The electrodes were considered to be dry for this measurement since the effect of sweating was minimal over this time. Once ECG acquisition from the dry textile electrodes was complete, the volunteer performed casual walking for 5 min wearing the smart ECG vest to induce sweating. ECG was then collected from the “wet” textile electrodes during different body movements and activities of daily living.
Before the start of each movement/activity, the subject rested for 5 min and was given a fresh hand towel to use to dry body sweating. Then, the textile electrodes used in the previous test were replaced with new dry textile electrodes. For the entire test duration, the standard EASI configuration and 3 mm thick, 70 mm2 textile electrodes were used to collect ECG.
Finally, ECGs from wet-gel electrodes (Nissha Medical Technologies, NY, United States) were collected using the e-textile ECG monitor during different body movements (yawning, deep breathing, sideways, and up movements) and activities of daily living (sitting/standing from a chair and climbing stairs) and signal quality was compared to that of the 3 mm thick 60 mm2 textile electrodes.
Comparing the E-Textile ECG to the Traditional Holter Monitor
ECG was acquired simultaneously from the e-textile ECG monitor (using 3 mm thick, 70 mm2 textile electrodes) and from a reference standard 3-lead Holter monitor (SEER light ambulatory ECG from General Electric Healthcare, Chicago, Illinois, U.S.; using wet-gel commercial electrodes). According to the reference Holter monitor user documentation, channel one is the reference lead and is used to acquire modified V5 (mV5). A modified V1 (mV1) is obtained through channel two. It is possible to collect either modified V3 (mV3), modified aVF (maVF), or modified Z (mZ) ECG based on the lead placement connected to channel three (30)2. The modified maVF arrangement was selected as the lead placement during the maVF ECG does not coincide with any of the EASI ECG electrode positions.
Data Analysis
ECG from the e-textile monitor was sampled at 4,000 samples per second (sps), recorded at 200 sps with a frequency range from 0 to 100 Hz at −3 dB level, and wirelessly transmitted to a host PC. Data were analyzed using MATLABTM 2017Ra software (The MathWorks Inc., Natick, MA, U.S.).
The ECG collected from the SEER Holter monitor was exported to MIT Signal Format upon completing the experiments. The data were then converted to an excel file (CSV UTF comma delimited—*.csv) and Text (Tab delimited—*.txt) format using a MATLAB script for ease of manipulation. According to the header file, the ECG from the Holter monitor was recorded at 125 Hz. However, the proposed textile ECG monitor has a recording frequency of 200 samples per second. Therefore, the Holter EC was resampled to match the 200 Hz rate of the textile ECG.
To retain as much low-frequency noise as possible while removing the DC offset from the inadequate skin-electrode interface and the electrode half-cell potential (31), a first-order Butterworth high pass filter (Fc = 0.067 Hz) was used to block the zero-frequency interference into the acquired ECG signal.
Statistical Analysis
Wilcoxon Signed-Rank Test is the non-parametric form of the paired-sample t-test used to analyze samples where the data has unknown distribution. The Wilcoxon Test ranks the absolute values of the differences between the paired data in the two samples. It computes statistical values based on the number of negative and positive differences. If the resulting p-value is small (p < 0.05), it is safe to assume that the two samples have different distributions and reject the null hypothesis (32). In cardiac research, previous studies (13) validated the Wilcoxon Signed-Rank Test as a practical statistical tool to analyze ECG. As a result, the Wilcoxon Signed-Rank Test was used to compare results throughout the experiment. A p-value of < 0.05 was considered statistically significant.
Results
Results from the e-textile ECG experiments are presented in four themes below (A) Textile electrode characteristic, (B) Electrode placement, (C) Electrode condition, and (D) Comparison between the e-textile ECG monitor and the commercial Holter monitor.
Textile Electrode Characteristic
Effect of Electrode Area on Signal Quality
Figure 3 illustrates representative ECG strips from different size textile electrodes. Table 1 presents the SQI (ApEn, basSQI, and SNR) analysis results of experiments conducted to investigate the effect of electrode surface area on motion artifact.
Figure 3. Lead-II representative ECG traces from different size textile electrodes acquired during climbing stairs (top–40 mm2; middle–60 mm2 and bottom–70 mm2 textile electrodes).
Table 1. Results of SQI analysis based on lead-II ECGs from different size textile electrodes (40, 60, and 70 mm2).
The ECG from the 70 mm2 textile electrodes showed lower ApEn during yawning, deep breathing, and up movement and climbing stairs. In contrast, the ECG from the 60 mm2 textile electrodes showed lower ApEn during coughing. The ECG acquired from the 70 and 60 mm2 textile electrodes did not show significant ApEn difference during sideways movement, sitting/standing from a chair and climbing stairs.
Table 1 also compares the SNR values of the ECGs obtained from textile electrodes of three different surface areas (40, 60, 70 mm2). During deep breathing, the lead-II ECG from the 60 mm2 showed increased SNR. Acquiring ECG through the 70 mm2 textile sensors showed higher SNR compared to the smaller area textile electrodes.
Effect of Electrode Thickness (Electrode Padding) on Signal Quality
The signal quality parameters were computed to examine the influence of electrode thickness on the ECG signal quality during different body movements and activities of daily living. The results are presented in Table 2. As shown in Table 2, the ECG collected from the 10 mm thick textile electrodes performed poorly for all movements and activities (higher ApEn, lower basSQI, and lower SNR).
Table 2. Results of SQI analysis based on lead-II ECGs acquired from 3, 5, and 10 mm thick Textile electrodes.
Electrode Placement
Figure 4 presents the temporal plots of lead-II ECG acquired during sideways movement.
Figure 4. Lead-II representative sideways ECG (top—Anterior; middle—medial and bottom—posterior AI electrodes placement).
The basSQI, average R-peak amplitude and average ECG power were calculated to assess the power characteristics of the acquired ECG and are summarized in Table 3. Placing the textile electrodes far from the heart, on the posterior-axillary lines, reduced the amplitude of the collected ECG. Therefore, even though the medial placement showed higher noise in the low-frequency range, the basSQI values were better compared to the posterior placement (Table 3).
Table 3. Summary of the ECG characteristics based on “AI” electrodes placement.
ApEn analysis was conducted to confirm that the higher power content of the ECG acquired from the anterior axillary lines is, in fact, mainly from the ECG signal, and the results are summarized in Table 3. The anterior axillary electrode placement resulted in lower randomness in the acquired signal for the entire experiment. On the other hand, the ECG obtained from the medial-axillary lines showed a higher noise level for every test involving hand movement (sideways, up, sitting/standing from a chair, and climbing stairs).
Electrode Condition
Effect of Sweating on Signal Quality
The ApEn analysis results presented in Table 4 revealed that the ECG collected from dry textile electrodes exhibited higher randomness (higher ApEn) compared to the ECGs from wet textile sensors. The basSQI and SNR computation results of the ECG from dry and wet textile electrodes (Table 4) showed that the ECG acquired from the wet textile electrodes showed reduced noise in the low-frequency region.
Table 4. SQI analysis results of lead-II ECG from textile electrodes and commercial wet-gel electrodes using textile ECG monitor.
Comparison Between the Textile Electrodes and the Disposable Wet-Gel Electrodes
Quantitative signal quality parameters, including ApEn, basSQI, and SNR, were computed, and the results are presented in Table 4. The ECGs from the commercial wet-gel electrodes exhibited higher randomness (ApEn) and lower basSQI values compared to the wet-textile electrodes. However, ECGs from the commercial wet-gel electrodes showed higher SNR during deep breathing, sitting/standing from a chair, and climbing stairs (Table 4). Figure 5 presents a representative ECG acquired during up movement.
Figure 5. Lead-II representative ECG during up movement (top—wet textile electrodes and bottom—commercial wet-gel electrodes).
Comparison Between the E-Textile ECG Monitor and the Commercial Holter Monitor
Six seconds of representative ECG trances from the Holter and textile-based ECG are presented in Figure 6. Increased body movement (e.g., climbing stairs, Figure 6) forced the ECG to drift away from the isoelectric line.
Figure 6. V5 representative ECG during climbing stairs (top—Holter ECG and bottom—textile ECG monitor).
Table 5 compares the quantitative signal quality parameters. The Holter ECG revealed an increased interference in the low-frequency region of the ECG acquired, especially during sitting/standing activities (basSQI = 0.8067), lying on a bed (basSQI = 0.8687), climbing stairs (basSQI = 0.8874), and making a phone call from a mobile phone (basSQI = 0.9325). The significantly higher ApEn values of the ECGs from the reference ambulatory monitor (Table 5) supported the increased randomness of the Holter ECGs compared to the ECGs from the proposed textile-based ECG monitor.
Table 5. SQI analysis results of lead V5 ECG from the reference Holter monitor (using wet-gel commercial electrodes) and the textile ECG monitor (using textile electrodes).
The SNR analysis (Table 5) further confirmed the higher baseline drifts within the reference Holter monitor during the two activities (sitting/standing from a chair and climbing stairs) compared to the ECG acquired from the proposed textile-based ECG monitor during the same sequence of body movements. However, the textile ECGs collected during sideways movement showed lower signal power.
Table 6 summarizes the QRS duration and QTc measurements from ECGs collected from both the reference Holter monitor and the proposed textile-based ECG monitor. There was no difference in the number of normal QRS intervals between the two systems. However, the QTC measures were different between the two systems when the subject was sitting in a chair. For the signals from the Holter monitor, 58 of the 262 QTC intervals were identified as long QTC (>0.45 s), and one was a short QTC interval (<0.36 s). However, 261 of the 262 QTC intervals from the textile-based ECG were detected as normal (Table 6 top). For both the Holter monitor and the textile-based ECG, one QTC interval was missing. The ECG was then denoised using a 2nd order high pass Butterworth filter (fc = 0.67 Hz) in a MATLAB environment and the QTC intervals were computed again. All 58 QTC intervals identified as long QTC within the reference ambulatory monitor were classified as normal QTC after denoising. Moreover, the missed T-wave was recovered in both the Holter and textile ECGs (Table 6 bottom).
Table 6. Summary of the QRS duration and QTC intervals of ECG from the Holter monitor and textile-based ECG monitor during different body movements and activities of the daily living.
Discussion
The aim of this study was to determine whether signals produced by an e-textile ECG monitor with textile electrodes in an EASI configuration are of sufficient quality to be used for cardiac monitoring. Specific objectives were to investigate the effect of the textile electrode characteristics, placement, and condition on signal quality, and finally to compare results to a reference ECG obtained from a current clinical standard the Holter monitor.
Textile Electrode Characteristic
The relation between the size of the electrodes and the ECG quality was studied. Results showed that the bigger the textile electrodes' size, the better the signal quality and the lower the approximate entropy (randomness of the signal). This finding is in agreement with that reported by Ueno et al. (33). Throughout the experiment, the ECG from the 70 mm2 resulted in a higher peak ECG signal for all body movements and daily activities except for deep breathing, where the ECG collected via the 60 mm2 textile electrodes showed slightly higher SNR. The increased ECG amplitude and higher signal power for an increased electrode area also agree with previous studies (34–36).
In a previous study, Cömert and Hyttinen (37) used a 4 mm thick cushion padding structure to support their textile electrodes. The authors showed that the electrode support structure and padding increased the stability of the skin-electrode interface and distributed the compressive force uniformly across the electrode. Moreover, a soft support structure has been shown to produce less noise as it allows the textile electrode to follow the underlying anatomy (37). In this study, 3, 5, and 10 mm thick textile electrodes were constructed using a soft support structure made of Statfree® conductive polyurethane foam.
Throughout the trial, ECG signals from the 3 and 5 mm textile electrodes showed higher signal power, lower randomness, and decreased motion artifact aligning with Comert and Hyttinen's 2015 study (37), where they showed a positive relationship between electrode padding and signal quality using a 4 mm thick padding. In another study, Cömert et al. (38) examined the effect of different thicknesses and types of padding using 6, 9, 13, 14, and 16 mm thick electrodes where the padding was made of two different grades of SunMate memory foam and Poron XRD impact protection cushion. The authors reported the positive effect of padding on signal quality. However, the padding that resulted in the best ECG quality was not clearly stated.
In this study, increasing the padding thickness beyond 5 mm showed decreased signal quality. For example, during climbing stairs, the ECG from the 10 mm thick textile sensors performed worst. This may be a result of the thicker textile electrodes (thicker padding, e.g., 10 mm thick textile electrodes) shifting position and sliding when subjected to movement more so than the thinner (e.g., 3 and 5 mm) textile electrodes. Moreover, Cömert et al. (38) used a different technique to acquire the ECG (electrode was placed on the upper arm and was subjected to different magnitude pressure from 5 to 25 mmHg) and a different material to make the support structure. In our experiment, the electrodes were placed along the EASI configuration, and the support structure was made of Statfree® conductive polyurethane foam. Hence, it is difficult to compare the results directly.
Electrode Placement
Based on the EASI configuration, placing the “A” and “I” electrodes at the anterior axillary line showed a lower ApEn. At the same time, during sideways and up movement, the medial axillary and posterior “AI” placement showed higher randomness (an increased ApEn) in the acquired ECG. As the hands were moved side to side (sideways) and raised above the head and then moved back (up movement), there was a high chance of the arms touching the electrodes placed under the armpit and on the posterior axillary lines, resulting in an unstable skin-electrode interface. This continuous impedance-change induced low-frequency interference in the acquired ECGs.
Moreover, moving the electrodes from the anterior-axillary to the posterior-axillary line diminished the R-wave ECG amplitude, reduced the power contained within the acquired ECG, and increased low-frequency noise. From an electrophysiology perspective, where the body is assumed to be a volume conductor (39), the further the sensors from the source (the heart), the higher the impedance of the volume conductor (39, 40). Therefore, it is unsurprising that the amplitude of the ECG collected with the AI electrodes on the medial axillary line is greater than the ECG collected at the posterior axillary line.
Electrode Condition
Wet textile electrodes (from sweating) were compared to dry counterparts. They were found to perform better as the dry textile electrodes drift easily, change position, and are susceptible to motion artifact during physical activities. Moreover, the performance of the wet textile electrodes was comparable to that of commercial wet-gel electrodes. Previous studies support this result. Pani et al. (41) used poly (3,4-ethylene dioxythiophene): poly (styrene sulfonate) textile electrodes to compare dry textile electrodes, wet textile electrodes, and commercial Ag/AgCl electrodes during different daily activities. The authors showed that the dry textile electrodes performed poorly, especially during physical activities. However, the wet textile electrodes were as good as the Ag/AgCl commercial electrodes. When evaluated based on a QRS detector, the wet textile electrodes performed better than the commercial Ag/AgCl electrodes.
Marozas et al. (34) compared commercial Ag/AgCl electrodes to wet textile electrodes in exercise ECG. The authors concluded that the textile electrodes showed significant noise in the low-frequency band (0–0.67 Hz) while textile electrodes are less prone to broadband noise (0–250 Hz) compared to the Ag/AgCl electrodes. We did not see the same level of low-frequency noise; however, results cannot be directly compared as we did not experiment on exercise ECG. Also, Marozas et al. (34) used three electrodes placed on the thorax area 25 cm apart, where in our case we used the EASI electrode configuration. However, the analog front-end of our hardware has been carefully designed to minimize low-frequency distortion, which might be why we did not observe intense low-frequency noise from the wet textile electrodes.
Comparison Between the E-Textile ECG Monitor and the Commercial Holter Monitor
The performance of the textile-based ECG monitor was compared against the traditional Holter monitor. Channel one (modified V5) from the Holter monitor and the V5 ECG from the textile-based ECG monitor were used to analyze the data. In the time domain plots, there was no significant difference between the ECGs acquired from the Holter monitor and the textile-based ECG monitor. Even from the noisy recording, it was possible to identify the QRS complexes. The main problem seen on the time traces were baseline drift. In both the Holter monitor and the textile-based ECG monitor the motion artifact within the QRS band (5–15 Hz) was minimal as confirmed by the SQI values. However, the ECGs from the Holter monitor showed an increased low-frequency noise, and hence lower basSQI values during a phone call, sitting / standing from a chair, lying on a bed, and climbing stairs.
In summary, compared to the body movements (e.g., deep breathing), the daily activities (e.g., sitting / standing from a chair) resulted in greater low-frequency interference within the ECG acquired from the Holter monitor. The reference ambulatory monitor and the smart ECG vest were used simultaneously. In this regard, for an increased activity like sitting / standing from a chair, the smart ECG vest might be touching the Holter lead wires and hence introducing an increased noise within the Holter ECG.
The precise delineation of the QRS duration and QTC interval is important to detect cardiac episodes. In this regard, the QRS durations and the QTC intervals were extracted from the Holter ECGs and the textile-based ECGs, and the results compared. Based on the QRS and QTC analysis, there was no significant difference between the Holter monitor and the textile-based ECG monitor. However, the textile-based ECG monitor showed higher accuracy than the Holter monitor for the ECG collected when the participant sat quietly. Previous studies showed that ECGs acquired during upright position showed a decreased amplitude in the ST-segments (42), T (43), and Q (44) waves. Moreover, Yokus and Jur (45) compared the textile and wet-commercial electrodes and reported that ECGs collected from textile electrodes during sitting showed a higher SNR. As a result, sitting ECGs from the Holter monitor might be prone to low-frequency noise that affects the lower amplitude Q and T waves. The peak detection algorithm might be an additional contributing factor (34, 46).
Limitations
The major limitation of our study is that data were only collected from a single healthy participant. Even though ethics approval was obtained from the Flinders University Social and Behavioral Research Ethics Committee (SBREC: project code – 8490), it was not possible to recruit and collect data from more participants or cardiac patients due to the outbreak of the COVID-19 pandemic. As a result, given a unisex design of the ECG vest, the effect of different body sizes, body hair, skin type, and gender on ECG quality and the presence or absence of skin irritation due to textile electrodes were not studied.
Conclusion and Implications for Further Research
This study reports on the testing and evaluation of a wireless and wearable EASI-based e-textile ECG monitor. Optimal electrodeposition remains an active area of research for quality ECG transduction. In this regard, the best electrodeposition for the EASI configuration was studied, where the results could be extended for the traditional EASI lead system ECG. Placing the “A” and “I” electrodes on the left and right anterior axillary point, respectively, showed higher signal quality compared to the standard EASI electrode placement.
The preliminary results revealed that there was no significant signal quality difference between the traditional Holter monitor and the e-textile ECG monitor. The standard ambulatory monitor utilizes sticky wet-gel electrodes where the ECG quality deteriorates over time due to the drying of the gel interface. Moreover, the ECG lead wires reduced the comfort of the users. On the other hand, the textile-based ECG monitor has embedded wires and textile electrodes.
The use of the EASI configuration combined with the wearable and wireless design of the e-textile ECG monitor could support long-term ambulatory monitoring of cardiac patients and increase access to cardiac rehabilitation via telemonitoring. The intuitive design of the ECG vest will significantly reduce the time needed to train the users. No assistance is required to put on/off the smart ECG vest. Therefore, it will also lower diagnosis errors due to misplaced electrodes. Further research is needed to validate the e-textile ECG monitor in a larger trial and on a cardiac population.
Data Availability Statement
The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.
Ethics Statement
The studies involving human participants were reviewed and approved by Flinders University Social and Behavioral Research Ethics Committee (SBREC: Project Code – 8490). The patients/participants provided their written informed consent to participate in this study.
Author Contributions
MT conceived the original idea, contributed to the study design, analyzed the data, and wrote the manuscript with support from DH, RC, and KR. All authors contributed to the data analysis, interpretation, and to drafting the article.
Funding
Flinders University supported this project. CINOP Global and Addis Ababa Institute of Technology supported MT through Nuffic funded NICHE project NICHE/ETH/246. Development of the ECG vest was supported by the Tom Simpson Trust Fund and the National Heart Foundation of Australia. KR was funding the publication cost.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher's Note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Acknowledgments
MT thank the staff at the Cardiac Clinic, Flinders Medical Centre, especially May Siew and Rosy Tirimacco, for their assistance during the data collection, and Michael Liedlgruber for his guidance during the data analysis.
Footnotes
1. ^Available at: https://archive.physionet.org/physiotools/ApEn/.
2. ^Available at: http://apps.gehealthcare.com/servlet/ClientServlet.
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Keywords: ambulatory cardiac monitoring, EASI ECG, electronic-textile electrodes, Holter monitoring, smart fabrics, wearable device, wearable sensors
Citation: Teferra MN, Hobbs DA, Clark RA and Reynolds KJ (2021) Preliminary Analysis of a Wireless and Wearable Electronic-Textile EASI-Based Electrocardiogram. Front. Cardiovasc. Med. 8:806726. doi: 10.3389/fcvm.2021.806726
Received: 01 November 2021; Accepted: 29 November 2021;
Published: 20 December 2021.
Edited by:
David Duncker, Hannover Medical School, GermanyReviewed by:
Martin Manninger, Medical University of Graz, AustriaSotirios Nedios, Helios Kliniken, Germany
Copyright © 2021 Teferra, Hobbs, Clark and Reynolds. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Meseret N. Teferra, meseret.teferra@flinders.edu.au