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ORIGINAL RESEARCH article

Front. Cell. Infect. Microbiol.
Sec. Biofilms
Volume 15 - 2025 | doi: 10.3389/fcimb.2025.1535539
This article is part of the Research Topic Novel Strategies for Caries Control View all articles

Exploration of the primary antibiofilm substance and mechanism employed by Lactobacillus salivarius ATCC 11741 to inhibit biofilm of Streptococcus mutans

Provisionally accepted
Nan Ma Nan Ma 1Wei Yang Wei Yang 2*Bairu Chen Bairu Chen 3*Meihua Bao Meihua Bao 3*Yimin Li Yimin Li 3*Meng Wang Meng Wang 3*Xiaopeng Yang Xiaopeng Yang 2*Junyi Liu Junyi Liu 4*Chengyue Wang Chengyue Wang 3*Lihong Qiu Lihong Qiu 5*
  • 1 Department of Periodontics, Affiliated Stomatology Hospital of Jinzhou Medical University, jinzhou, China
  • 2 Department of Pedodontics, Affiliated Stomatology Hospital of Jinzhou Medical University, jinzhou, China
  • 3 Department of Prosthetics, Affiliated Stomatology Hospital of Jinzhou Medical University, jinzhou, China
  • 4 Jinzhou Medical University, Jinzhou, Liaoning Province, China
  • 5 Department of Endodontics, School and Hospital of Stomatology, China Medical University, shenyang, China

The final, formatted version of the article will be published soon.

    Lactobacillus salivarius serves as a probiotic potentially capable of preventing dental caries both in vitro and in vivo. This study focused on understanding the key antibiofilm agents and the mechanisms of action of the Lactobacilli supernatant against Streptococcus mutans. The findings indicated that the supernatant derived from Lactobacillus salivarius could inhibit biofilm formation of Streptococcus mutans at different time. Through transcriptome analysis, we discovered that the cell-free supernatant reduced biofilm formation, by suppressing phosphoenolpyruvatedependent phosphotransferase systems along with two ATP-binding cassette transporters, rather than directly affecting the genes that code for glucosyltransferases, additionally, the supernatant was observed to diminish the expression of genes linked to two-component systems, polyketides/non-ribosomal peptides, acid stress response, quorum sensing, and exopolysaccharide-formation. Non-targeted LC-MS/MS analysis was employed to discover a variety of potential active compounds present in the cellular filtrate of Lactobacillus salivarius that hinder the growth of Streptococcus mutans, including phenyllactic acid, sorbitol, and honokiol. In summary, our findings support the evaluation of Lactobacillus salivarius as a promising oral probiotic aimed at hindering the formation of biofilms by cariogenic pathogens and the development of dental caries.

    Keywords: Streptococcus mutans1, transcriptomics2, metabolomics3, biofilm4, Dental Caries5, Lactobacillus salivarius6

    Received: 27 Nov 2024; Accepted: 14 Jan 2025.

    Copyright: © 2025 Ma, Yang, Chen, Bao, Li, Wang, Yang, Liu, Wang and Qiu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Wei Yang, Department of Pedodontics, Affiliated Stomatology Hospital of Jinzhou Medical University, jinzhou, China
    Bairu Chen, Department of Prosthetics, Affiliated Stomatology Hospital of Jinzhou Medical University, jinzhou, China
    Meihua Bao, Department of Prosthetics, Affiliated Stomatology Hospital of Jinzhou Medical University, jinzhou, China
    Yimin Li, Department of Prosthetics, Affiliated Stomatology Hospital of Jinzhou Medical University, jinzhou, China
    Meng Wang, Department of Prosthetics, Affiliated Stomatology Hospital of Jinzhou Medical University, jinzhou, China
    Xiaopeng Yang, Department of Pedodontics, Affiliated Stomatology Hospital of Jinzhou Medical University, jinzhou, China
    Junyi Liu, Jinzhou Medical University, Jinzhou, 121001, Liaoning Province, China
    Chengyue Wang, Department of Prosthetics, Affiliated Stomatology Hospital of Jinzhou Medical University, jinzhou, China
    Lihong Qiu, Department of Endodontics, School and Hospital of Stomatology, China Medical University, shenyang, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.