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ORIGINAL RESEARCH article

Front. Cell. Infect. Microbiol.
Sec. Clinical Microbiology
Volume 14 - 2024 | doi: 10.3389/fcimb.2024.1526633
This article is part of the Research Topic Advances in the Diagnosis and Management of Infectious Diseases View all 7 articles

Plasma Epstein-Barr Virus DNA Load for Diagnostic and Prognostic Assessment in Intestinal Epstein-Barr Virus Infection

Provisionally accepted

The final, formatted version of the article will be published soon.

    The prospectiveProspective application of plasma Epstein-Barr virus (EBV) DNA load as a noninvasive measure of intestinal EBV infection remains unexplored. This study aims to identifyaims to pinpoint ideal threshold levels for plasma EBV DNA loads in the diagnosis and outcome prediction of intestinal EBV infection, particularly in cases of primary intestinal lymphoproliferative diseases and inflammatory bowel disease (IBD).Methods: Receiver operating characteristic (ROC) curves werehave been examined to determine suitable thresholds for plasma EBV DNA load in diagnosing intestinal EBV infection and predicting its prognosis.Results: 108 patients were retrospectively assigned to the test group, whileand 56 patients were included in the validation group. Plasma EBV DNA loads were significantly higher in the intestinal EBV infection group compared to the non-intestinal EBV infection group (Median: 2.02 × 10 2 copies/mL, interquartile range [IQR]: 5.49 × 10 1 -6.34×10 3 copies/mL versus 4.2×10 1 copies/mL, IQR: 1.07 ×10 1 -6.08×10 1 copies/mL; P < 0.0001). , with median values of 2.02 × 10 2 copies/mL and 4.2 × 10 1 copies/mL, respectively. Plasma EBV DNA levels at 9.21×10 1 and 6.77×10 1 copies/mL were proved beneficial for the identification and alongside prognostication in intestinal EBV infection, respectively. Values of 0.82 and 0.71 were yielded by the area under the ROC curve (AUC) in the test cohort, corresponding to sensitivities of 84.38% (95% confidence interval [95%CI]: 68.25%-93.14% ) and 87.5% (95%CI: 69%-95.66%), specificities of 83.33% (95%CI: 64.15%-93.32% ) and 68.09% (95%CI: 53.83%-79.6%), positive predictive values (PPV) of 87.1% (95%CI: 71.15%-94.87%) and

    Keywords: Epstein-Barr virus DNA load, diagnosis, prognosis, Inflammatory Bowel Diseases, primary intestinal lymphoproliferative diseases, intestinal Epstein-Barr Virus infection

    Received: 12 Nov 2024; Accepted: 16 Dec 2024.

    Copyright: © 2024 Ma, Jiang, Li, Zeng, Wu, Cheng, Lin, Pang, Yin, Jia, Li and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Hu Zhang, West China Hospital, Sichuan University, Chengdu, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.