Skip to main content

ORIGINAL RESEARCH article

Front. Cell. Infect. Microbiol.
Sec. Extra-intestinal Microbiome
Volume 14 - 2024 | doi: 10.3389/fcimb.2024.1517125
This article is part of the Research Topic Microbiome-based metabolomics revolution in health and microbial metabolites redefinition View all 8 articles

Identification of Serotype O3b and High-Risk Clone ST37 of Klebsiella pneumoniae Revealed by Comparative Genomic Analysis

Provisionally accepted
Gopalan Sivaraman Gopalan Sivaraman 1Sudha Sajeev Sudha Sajeev 1Visnuvinayagam Sivam Visnuvinayagam Sivam 1Raja Swaminathan T Raja Swaminathan T 1Rakshit Ojha Rakshit Ojha 2Bibek Ranjan Shome Bibek Ranjan Shome 2Mark Holmes Mark Holmes 3Thanigaivel Sundaram Thanigaivel Sundaram 4Ramesh MD Ramesh MD 5Saranya Vinayagam Saranya Vinayagam 6Suseela Meesala Suseela Meesala 7Tabarak Malik Tabarak Malik 8*PAVAN KUMAR DARA PAVAN KUMAR DARA 4
  • 1 Central Institute of Fisheries Technology (ICAR), Kochi, Kerala, India
  • 2 National Institute of Veterinary Epidemiology And Disease Informatics (ICAR), Bengaluru, Karnataka, India
  • 3 University of Cambridge, Cambridge, England, United Kingdom
  • 4 SRM Institute of Science and Technology, Chennai, India
  • 5 University of Tarapacá, Arica, Tarapaca, Chile
  • 6 Saveetha Medical College & Hospital, Chennai, Tamil Nadu, India
  • 7 Vikrama Simhapuri University, Nellore, Andhra Pradesh, India
  • 8 Jimma University, Jimma, Oromia, Ethiopia

The final, formatted version of the article will be published soon.

    Background: Epidemiological risk factors such as the demography of a place, environment, food, livestock, and companion animals are known sources of Klebsiella pneumoniae infection. The Whole Genome Sequence (WGS) has become a powerful tool for complementing traditional microbiological characterization of foodborne pathogens. Moreover, the K. pneumoniae has several species complex (KpSC) and is very difficult to differentiate using routine microbiological methods. The present study aims to investigate the prevalence of K. pneumoniae in fish available in the retail market using WGS.Methods: Isolation of K. pneumoniae, Identification of K. pneumoniae isolates and determination of the minimum inhibitory concentration (MIC) was performed, Whole-genome sequencing of K. pneumoniae genomes, Phylogenomic analysis for visual comparison of the genomes, nonhuman origin that were submitted from India to the NCBI database were downloaded and included in the comparative analysisThe findings showed that many (Antibiotic resistant genes) ARGs are prominent, including acrD,

    Keywords: K. pneumoniae, Antibiotic resistant genes, whole genome sequencing, serotype, High-risk clone

    Received: 25 Oct 2024; Accepted: 13 Dec 2024.

    Copyright: © 2024 Sivaraman, Sajeev, Sivam, T, Ojha, Shome, Holmes, Sundaram, MD, Vinayagam, Meesala, Malik and DARA. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Tabarak Malik, Jimma University, Jimma, Oromia, Ethiopia

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.