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ORIGINAL RESEARCH article

Front. Cell. Infect. Microbiol., 15 September 2023
Sec. Biofilms
This article is part of the Research Topic Mechanobiology of biofilms and associated host -pathogen interactions View all 6 articles

The great divide: rhamnolipids mediate separation between P. aeruginosa and S. aureus

Jean-Louis Bru&#x;Jean-Louis Bru1†Summer J. Kasallis,&#x;Summer J. Kasallis1,2†Rendell ChangRendell Chang3Quantum ZhuoQuantum Zhuo2Jacqueline NguyenJacqueline Nguyen3Phillip PhamPhillip Pham1Elizabeth WarrenElizabeth Warren4Katrine WhitesonKatrine Whiteson1Nina Molin Hyland-KroghsboNina Molin Høyland-Kroghsbo5Dominique H. LimoliDominique H. Limoli4Albert Siryaporn,*Albert Siryaporn1,2*
  • 1Department of Molecular Biology & Biochemistry, University of California, Irvine, Irvine, CA, United States
  • 2Department of Physics & Astronomy, University of California, Irvine, Irvine, CA, United States
  • 3School of Biological Sciences, University of California, Irvine, Irvine, CA, United States
  • 4Department of Microbiology and Immunology, University of Iowa, Iowa City, IA, United States
  • 5Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark

The interactions between bacterial species during infection can have significant impacts on pathogenesis. Pseudomonas aeruginosa and Staphylococcus aureus are opportunistic bacterial pathogens that can co-infect hosts and cause serious illness. The factors that dictate whether one species outcompetes the other or whether the two species coexist are not fully understood. We investigated the role of surfactants in the interactions between these two species on a surface that enables P. aeruginosa to swarm. We found that P. aeruginosa swarms are repelled by colonies of clinical S. aureus isolates, creating physical separation between the two strains. This effect was abolished in mutants of S. aureus that were defective in the production of phenol-soluble modulins (PSMs), which form amyloid fibrils around wild-type S. aureus colonies. We investigated the mechanism that establishes physical separation between the two species using Imaging of Reflected Illuminated Structures (IRIS), which is a non-invasive imaging method that tracks the flow of surfactants produced by P. aeruginosa. We found that PSMs produced by S. aureus deflected the surfactant flow, which in turn, altered the direction of P. aeruginosa swarms. These findings show that rhamnolipids mediate physical separation between P. aeruginosa and S. aureus, which could facilitate coexistence between these species. Additionally, we found that a number of molecules repelled P. aeruginosa swarms, consistent with a surfactant deflection mechanism. These include Bacillus subtilis surfactant, the fatty acids oleic acid and linoleic acid, and the synthetic lubricant polydimethylsiloxane. Lung surfactant repelled P. aeruginosa swarms and inhibited swarm expansion altogether at higher concentration. Our results suggest that surfactant interactions could have major impacts on bacteria-bacteria and bacteria-host relationships. In addition, our findings uncover a mechanism responsible for P. aeruginosa swarm development that does not rely solely on sensing but instead is based on the flow of surfactant.

Introduction

Pseudomonas aeruginosa and Staphylococcus aureus are opportunistic pathogens that colonize the skin, eyes, and lungs, where they can contribute to the development of a range of illnesses (Lyczak et al., 2000; Howden et al., 2023). While a single species can dominate during infection, co-infections have been associated with worse patient outcomes (Camus et al., 2022). In particular, the two species commonly coinfect the lungs of cystic fibrosis patients (Salsgiver et al., 2016). Several factors produced by each species affect their ability to coexist. For example, 4-hydroxy-2-heptylquinoline N-oxide (HQNO), pyocyanin, and LasA protease produced by P. aeruginosa negatively affect S. aureus growth (Kessler et al., 1993; Hoffman et al., 2006; Biswas et al., 2009). Despite such factors, the species can coexist as mixed biofilms (reviewed in (Hotterbeekx et al., 2017)). In addition, recent work has revealed that P. aeruginosa detects and responds to the presence of S. aureus in several ways. For example, the presence of S. aureus induces exploratory motility in P. aeruginosa microcolonies (Limoli et al., 2019) and P. aeruginosa detects S. aureus exoproducts including intermediate metabolites and molecules that modulate the iron starvation response (Zarrella and Khare, 2022).

Additional insight into how these species interact in host environments may be gained by considering their microenvironments. P. aeruginosa releases several molecules into its surroundings that improve its own fitness, including rhamnolipids (RLs), siderophores, and phenazines. The production of these molecules is regulated by the cell-to-cell communication process known as quorum sensing (QS), which enables groups of bacteria to coordinate collective behaviors by emitting and detecting QS molecules (Papenfort and Bassler, 2016). RLs have a significant impact on the P. aeruginosa microenvironment due to their surfactant properties, ubiquity, and multifunctional roles. RLs are glycolipids that consist of rhamnose and variable-length fatty acid moieties (Jarvis and Johnson, 1949; Abdel-Mawgoud et al., 2010). They are amphipathic, containing both hydrophilic (rhamnose) and hydrophobic (fatty acid) groups, and function as surfactants, which decrease surface tension at air-liquid interfaces and interfacial tension between two liquids. Due to their surfactant properties, RLs increase the solubility of hydrophobic molecules. This property improves the ability of P. aeruginosa to uptake otherwise poorly soluble molecules such as hydrocarbons, which can be used as nutrient sources (Beal and Betts, 2000; Noordman and Janssen, 2002; Abdel-Mawgoud et al., 2010). RLs additionally solubilize the P. aeruginosa QS molecule 2-heptyl-3-hydroxy-4-quinolone (PQS) (Collier et al., 2002; Calfee et al., 2005), which could enhance PQS diffusion and sensing. RLs can directly impact S. aureus through the formation of micelles that transport toxins into the bacterium, altering its biofilm development and QS gene expression, and functioning as an antimicrobial (Haba et al., 2003; Benincasa et al., 2004; Gdaniec et al., 2022; Saadati et al., 2022). Serving as an important factor in pathogenesis, RLs are found in relatively high abundance in infection environments such as the lung, where they promote biofilm formation and inhibit host immunity (Kownatzki et al., 1987; McClure and Schiller, 1992; Pamp and Tolker-Nielsen, 2007; Abdel-Mawgoud et al., 2010).

RLs have a critical role in the expansion of P. aeruginosa swarms, which are characterized by radially expanding dendritic patterns of densely packed cells that are referred to as tendrils (Caiazza et al., 2005). Cells within swarms are highly motile via flagellar activity, grow at a high density, and have high metabolic activity (reviewed in (Kearns, 2010)). Due to the rapid expansion of swarms across large distances, swarming has been widely described as a form of motility. RLs are produced in abundance by P. aeruginosa in swarms, where RLs expand radially from colonies and precede tendril formation (Siegmund and Wagner, 1991; Morris et al., 2011; Xavier et al., 2011). The spatial heterogeneity and abundance of RLs present in swarms make swarming an ideal system to investigate interactions between P. aeruginosa and S. aureus.

Recent work investigated the interaction between sub-populations of P. aeruginosa within swarms, finding that cells stressed by bacteriophage (phage) virus infection or antibiotics repel P. aeruginosa tendrils (Bru et al., 2019). This reorganization creates spatial separation between stressed and untreated populations, enabling “healthy” P. aeruginosa populations to physically avoid agents that induce stress. This collective stress response occurs through altered production of RLs, the QS-associated molecule PQS, and its precursor, 2-heptyl-4-quinolone (HHQ) (Figure 1A) (Bru et al., 2019). Untreated populations produce RLs that facilitate swarming. Phage or antibiotic-stressed populations increase the production of PQS, which signals the redirection of healthy tendrils away from the area of stress (Bru et al., 2019). A similar process could facilitate physical separation of P. aeruginosa from predators and competitors, facilitating coexistence between P. aeruginosa and other bacterial species. Many questions remain regarding how tendrils are steered away from PQS. For example, P. aeruginosa can sense PQS produced by the stressed populations through the QS receptor and transcriptional regulator PqsR (Wade et al., 2005). However, the mechanism by which the swarm alters its tendril motion in response to the detection of PQS is unknown. Additionally, it is not understood how prevalent the collective stress response is among strains of P. aeruginosa or other bacterial species including S. aureus.

FIGURE 1
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Figure 1 The effect of tobramycin on swarm repulsion by wild-type P. aeruginosa, mutants, and clinical isolates. (A) Schematic depicting relationship between the QS molecule 2-heptyl-3-hydroxy-4-quinolone (PQS), its precursor HHQ, and rhamnolipid (RL) production in P. aeruginosa on swarming plates. Untreated P. aeruginosa produces more RLs whereas antibiotic- or phage-stressed cells produce more PQS. (B) Schematic depicting swarm interaction assays in which P. aeruginosa is inoculated at the center and forms swarm tendrils that move outwards. (C) Swarm interaction assays in which wild-type (WT) P. aeruginosa was spotted at the center and test strains were spotted at satellite positions. Tobramycin (TOB) treatment was performed by mixing TOB with bacteria to a final concentration of 0.5 mg/mL and spotting 6 μL of the mixture onto the swarm plate. Images were acquired 18-20 hours following inoculation. Dashed lines indicate the boundaries of the initial inoculum. (D) Quantification of tendril repulsion by P. aeruginosa PA14 WT and mutant strains. Black dots represent the average radius from six satellite positions on a single plate, red bars indicate the average of three independent experiments, and error bars indicate standard deviation. (E) Quantification of tendril repulsion by P. aeruginosa clinical isolates from airways or skin wounds. Black dots represent the repulsion radius (cm) of individual satellite positions from the same plate and red bars indicate the average. Error bars indicate standard deviations. T-tests in (D) and (E) were performed as two-tailed distributions with unequal variance. *** denotes p < 0.001. T-tests in (E) were performed using individual satellite positions from the same plate. (F) Plot summarizing the number of clinical isolates that did not repel swarm tendrils (no repulsion) or exhibited repulsion at four or more out of six satellite positions (repulsion) in swarm interaction assays. Swarm assay images for the clinical isolates are shown in Figure S2A in the Supplementary Materials.

S. aureus modifies its microenvironment in part through the production of phenol-soluble modulins (PSMs), which are amphipathic peptides that have surfactant properties, antimicrobial activity, and inhibit host innate immunity (Kaito and Sekimizu, 2007; Cogen et al., 2010; Tsompanidou et al., 2011; Peschel and Otto, 2013). Multiple PSMs have been identified, including PSMα1 to PSMα4, PSMβ1 and PSMβ2, and PSMγ, also referred to as δ-toxin (Wang et al., 2007; Peschel and Otto, 2013). δ-toxins resemble PSMα and are encoded by the hld gene. PSMs can aggregate, which results in the formation of amyloid fibrils that have multiple functions, including the fortification of the matrix of S. aureus biofilms and increasing the cytotoxicity of PSMs (Schwartz et al., 2012; Tayeb-Fligelman et al., 2017; Zheng et al., 2018; Zaman and Andreasen, 2020; Kreutzberger et al., 2022). PSMs facilitate the expansion of S. aureus on surfaces through spreading and comet formation, a mechanism similar to the active motility mechanism known as gliding (Tsompanidou et al., 2013; Pollitt et al., 2015). The functional roles of PSMs and RLs and their impact on their microenvironments suggest that they could have a significant role in P. aeruginosa - S. aureus interactions.

Here, we investigate how P. aeruginosa swarm tendrils interact with clinical isolates of P. aeruginosa and S. aureus. We find that clinical P. aeruginosa strains that are stressed by the antibiotic tobramycin repel swarm tendrils, suggesting that the collective stress response may be prevalent among P. aeruginosa strains. Untreated S. aureus repels P. aeruginosa swarm tendrils, an effect which is abolished in S. aureus strains that are defective in the production of PSMs. We use Imaging of Reflected Illuminated Structures (IRIS) (Kasallis et al., 2023) to investigate how S. aureus repels swarm tendrils by tracking the movement of surfactant produced by P. aeruginosa. We find that S. aureus alters the flow of surfactant from P. aeruginosa swarms, creating a zone of surfactant exclusion which results in tendril repulsion. This effect is significantly reduced in S. aureus PSM mutants. Our results show that the surfactant layer, which is composed of RLs, mediates the repulsion of P. aeruginosa swarms away from S. aureus. In support of this mechanism, multiple molecules including PQS, surfactin produced by B. subtilis, and lung surfactant, also repel P. aeruginosa swarms.

Results

Collective stress response in P. aeruginosa clinical isolates

The collective stress response was previously reported in a limited number of P. aeruginosa strains (Bru et al., 2019). We investigated the prevalence of the collective stress response in clinical isolates of P. aeruginosa using swarm interaction assays in order to determine if this response could be of clinical relevance. In these assays, P. aeruginosa UCBPP-PA14 was inoculated as a single spot at the center of a plate containing swarm medium and test strains were inoculated at six satellite positions surrounding the center spot (Figure 1B). As the center swarm expands radially, the swarm tendrils approach and interact with the strain at the satellite positions (Figure 1B). This swarm interaction assay enables replication of a single condition or the testing of multiple conditions on the same swarm plate. When P. aeruginosa strain PA14 is spotted at satellite positions, it repels the center swarm due to the production of RLs (Bru et al., 2019), an effect that is abolished in ΔrhlAB strains, which do not produce RLs (Figures 1C, D). To elicit the collective stress response, antibiotics were supplied at satellite positions at above-MIC concentrations that slow growth but do not entirely inhibit it due to the diffusion of antibiotics away from the initial spot. Previous work showed that a number of antibiotics including gentamicin, kanamycin, and fosfomycin, elicited the collective stress response, causing antibiotic-stressed strains at satellite positions to repel untreated swarms (Bru et al., 2019).

We obtained 9 clinical isolates of P. aeruginosa that were isolated from airway or skin infections. The isolates exhibited varying degrees of resistance to kanamycin and fosfomycin, as evidenced by variable growth among strains that were treated with these antibiotics (Figure S1 in Supplementary Materials and (Bru et al., 2019)). In contrast, the strains were largely sensitive to tobramycin (TOB), as evidenced by the inhibition of growth or swarming in all but one TOB-treated satellite colonies (Figures 1C, E, F and Figure S2A in Supplementary Materials). TOB targets the bacterial 30S ribosomal subunit, blocks tRNA translocation (Ying et al., 2019), and induces the production of PQS in P. aeruginosa (Morales-Soto et al., 2018; Rieger et al., 2020). TOB-treated WT and ΔrhlAB strains at satellite positions were significantly inhibited for growth but repelled the untreated center swarm (Figures 1C, D). This result is consistent with TOB triggering the PQS-mediated collective stress response. We note that PQS alone is sufficient to repel the center swarm ((Bru et al., 2019) and Figure S2B in Supplementary Materials). TOB treatment of P. aeruginosa ΔrhlAB ΔpqsA, which lacks the ability to produce both RLs and PQS, failed to elicit tendril repulsion (Figures 1C, D), further supporting the dependence of the TOB-induced repulsive response on PQS. Center swarm tendrils collided with satellite positions containing only TOB, indicating that the repulsion is not due to the presence of TOB alone (Figures 1C, D). Together, the results suggest that TOB treatment of P. aeruginosa stimulates the collective stress response and repels swarms through the production of PQS. These results are consistent with the model that P. aeruginosa swarms produce RLs in non-stressed conditions but increase PQS production under stress from antibiotics (Figure 1A).

We assessed the TOB-induced stress response in the clinical isolates of P. aeruginosa (CIPa). Colonies were considered to possess at least partial ability to repel the center swarm if the tendrils did not come into contact with four or more of the satellite inoculum positions. In the absence of TOB, three of the clinical isolates (CIPa-4, CIPa-6, CIPa-8) repelled the center swarm (Figures 1E, F and Figure S2A in Supplementary Materials). We attribute the variability in repulsion among the clinical isolates to variations in swarm phenotypes, which are affected by the production of RLs and PQS in these strains. Treatment with TOB significantly reduced P. aeruginosa growth and caused at least partial repulsion of the swarm tendrils in 7 out of the 9 clinical isolates (Figures 1E, F and Figure S2A in Supplementary Materials). The only strains that repelled at less than four out of six satellite positions when treated with TOB were CIPa-3 and CIPa-9, the latter of which appeared to be TOB-resistant. The significant increase in the number of strains that repelled swarms due to TOB treatment (Figure 1F) is consistent with activation of a collective stress response and is consistent with a model in which PQS is produced in response to antibiotic stress in the TOB-sensitive clinical isolates tested. Together, these results suggest that the activity of the collective stress response is present in P. aeruginosa clinical isolates that cause disease in human airways and skin wounds.

S. aureus clinical isolates repel P. aeruginosa swarms

The collective stress response in P. aeruginosa could promote its survival in natural environments by separating the swarm population from areas that contain phage or antibiotics. We reasoned that P. aeruginosa could use a similar strategy to isolate itself from other bacterial species. We assessed the interaction between P. aeruginosa and S. aureus by inoculating P. aeruginosa at the swarm plate center and S. aureus at satellite positions (Figure 2A). Under these conditions, P. aeruginosa developed tendrils that moved outwards whereas S. aureus grew as colonies that did not expand spatially. We tested the interaction of P. aeruginosa with multiple methicillin-resistant S. aureus USA300 (LAC) clinical isolates, which we refer to as USA300 s1, USA300 s2, and USA300 JE2. These strains strongly repelled P. aeruginosa tendrils (Figures 2B, C), similar to antibiotic-stressed P. aeruginosa. The treatment of these S. aureus strains with TOB reduced colony growth but did not completely abolish it, similar to the effect on P. aeruginosa strains (Figures 1C, 2B). However, TOB treatment of S. aureus suppressed the repulsion of P. aeruginosa tendrils and enabled the tendrils to infiltrate the S. aureus colonies (Figures 2B, C). TOB treatment of the clinical strains of S. aureus (CISa), which were isolated from airways and skin wounds, largely reduced colony growth (Figure S3A and Movie S1 in Supplementary Materials). Growth was quantified for CISa strains for which the growth was difficult to discern visually and confirmed that TOB did not abolish growth (Figure S3B in Supplementary Materials). Similar to the USA300 strains, untreated CISa strains largely repelled P. aeruginosa tendrils (9 out of the 10 clinical isolates) and TOB treatment largely suppressed tendril repulsion (Figures 2D, E and Figure S3A in Supplementary Materials).

FIGURE 2
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Figure 2 The effect of tobramycin on swarm repulsion by wild-type S. aureus and clinical isolates. (A) Schematic indicating swarm interaction assay in which P. aeruginosa and S. aureus are inoculated at the center and satellite positions respectively, and P. aeruginosa swarm tendrils move outward towards the satellite positions. (B) Swarm interaction assays in which WT P. aeruginosa and WT S. aureus strains were spotted at satellite positions. TOB treatment was performed by mixing TOB with bacteria to a final concentration of 0.5 mg/mL and spotting 6 μL of the mixture onto the swarm plate. Plates were imaged 18-20 hours following inoculation. (C) Quantification of repulsion by S. aureus USA300 strains. Black dots represent the average repulsion radius from six satellite positions, red bars indicate the average of three independent experiments, and error bars indicate standard deviation. (D) Quantification of repulsion by S. aureus clinical isolates from airways or skin wounds. Black dots represent the repulsion radius of individual satellites from the same plate and red bars indicate the average. Error bars indicate standard deviation. T-tests in (C, D) were performed as two-tailed distributions with unequal variance. *** denotes p < 0.001. (E) Plot summarizing the number of clinical isolates that did not repel swarm tendrils (no repulsion) or exhibited repulsion at four or more out of six satellite positions (repulsion) in swarm interaction assays. Swarm assay images for the clinical isolates are shown in Figure S3A in the Supplementary Materials.

Whereas TOB treatment of P. aeruginosa caused tendril repulsion, this treatment of S. aureus suppressed tendril repulsion. Thus, P. aeruginosa is repelled by antibiotic stress of its own species, but not by antibiotic stress of its competitor S. aureus. This result suggests that the collective stress response is beneficial for only its own species. These findings raise the question of how S. aureus repels P. aeruginosa tendrils. While tendrils are repelled by antibiotic-stressed P. aeruginosa via the production of the QS molecule PQS, S. aureus does not contain the genes necessary for PQS synthesis. We hypothesized that S. aureus produces another QS-associated molecule that alters tendril direction.

PSM fibrils facilitate repulsion of P. aeruginosa swarms

We investigated the potential role of the QS-regulated PSMs produced by S. aureus in the repulsion of P. aeruginosa tendrils. We assessed the extent of repulsion by the S. aureus Δpsmα mutant. Whereas WT S. aureus strongly repelled P. aeruginosa swarms (Figures 2B, C), the Δpsmα mutant was deficient in repulsion, with P. aeruginosa tendrils infiltrating these colonies (Figure 3A). The USA300 Δpsmα Δhld mutant, which does not produce PSMα or the δ-toxin, were also deficient in repelling P. aeruginosa swarms (Figure 3A). These findings suggest that PSMα peptides significantly contribute to the repulsion of P. aeruginosa swarms.

FIGURE 3
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Figure 3 Dependence of P. aeruginosa tendril repulsion on phenol-soluble modulins (PSMs). (A) Swarm interaction assays in which WT P. aeruginosa was spotted at the center and WT S. aureus strains (USA300 s1 and s2) or their respective Δpsmα or Δpsmα Δhld mutants were spotted at the satellite positions. The images for WT S. aureus are the same as those in Figure 2B and are shown here for reference. (B) Copper grids were placed at the same location as or adjacent to inoculums of WT S. aureus or the Δpsmα Δhld mutant on swarm plates and incubated 18 to 20 hours (small images). Grids were imaged using transmission electron microscopy (TEM) (large images). Fibrils (red arrows) were observed at all positions on the grids in the vicinity of the WT S. aureus colony. No fibrils were observed at positions that were 1.5 mm or greater from the edge of the S. aureus Δpsmα Δhld colony. Scale bars indicate 3 mm and 500 nm in the small and large images, respectively.

S. aureus PSMs can form amyloid fibrils that support biofilm structures (Tayeb-Fligelman et al., 2017; Zheng et al., 2018; Zaman and Andreasen, 2020; Kreutzberger et al., 2022). We considered the possibility that PSM fibrils could mediate P. aeruginosa repulsion. First, we investigated the formation of fibrils by PSMs on the surface of the swarm plate surface using transmission electron microscopy (TEM). Carbon-coated copper TEM grids were placed at or adjacent to S. aureus inoculation sites at satellite positions and were incubated for the same period of time as the swarming assays to allow for PSM production and spreading. In WT S. aureus, we observed fibrous structures that were consistent with amyloid fibrils. The fibrils were present at distances up to 2.5 mm from the colony edge (Figure 3B and Figure S4A in Supplementary Materials). In contrast, no fibrils were observed in the Δpsmα Δhld mutant beyond the colony edge (Figure 3B and Figure S4B in Supplementary Materials). These observations suggest that PSMs form a layer of amyloid fibrils that surround WT S. aureus colonies.

Deflection of the surfactant layer causes tendril repulsion

To uncover the mechanism underlying the repulsion of tendrils by PSM amyloid fibrils, we considered two models that could explain tendril repulsion by PSMs: (1) cellular sensing of the PSMs and (2) a physicochemical mechanism that alters the development of tendrils. In the first model, P. aeruginosa could sense PSM fibrils and alter its motility away from them. For example, the P. aeruginosa pilus chemoreceptor PilJ is involved in sensing S. aureus and it is possible that it could detect PSMs (Yarrington et al., 2022). In the second model the repulsion of tendrils does not require detection by P. aeruginosa, but is due to physicochemical interactions between the amphipathic PSMs and the swarm. In this model, PSMs form a fibril boundary around S. aureus that repels approaching tendrils by altering the spatial distribution of the RLs near the P. aeruginosa tendrils.

To differentiate between these two models and better understand tendril repulsion, we performed a series of experiments using molecules with a range of hydrophobicities. We hypothesized that hydrophobicity would be an important determinant in tendril repulsion. We selected non-polar molecules with low volatilities to ensure that they remained on the swarm plate throughout the duration of the assays. Long-chain carbon molecules fit these criteria, including: oleic acid and linoleic acid (fatty acids), glyceryl trioleate and glyceryl trilinoleate (triglyceride forms of the fatty acids), Triton X-100 and Tween-20 (surfactants), and the synthetic liquid lubricant polydimethylsiloxane (PDMS) (Figure 4A).

FIGURE 4
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Figure 4 Repulsion of P. aeruginosa swarms by hydrophobic molecules. (A) Chemical structures of hydrophobic molecules that were tested for repulsion in swarm interaction assays. (B) Schematic indicating how contact angles were measured using images from a contact angle goniometer. Images of droplets on an oleophobic surface are shown for water and 1000 cSt PDMS, which gave the highest and lowest contact angles, respectively. (C) Contact angle measurements for each of the molecules. Repulsion or lack of repulsion was assessed using the data in Figures 4D and 1C. Points indicate the average measurement of the left and right sides of each droplet. Red lines indicate the average (n=6) and error bars indicate standard deviation. Representative droplet images can be found in Figure S5A in the Supplementary Materials. (D) Swarm interaction assays in which WT P. aeruginosa and test molecules were spotted at the center or satellite positions, respectively. Images were acquired 15 hours following inoculation. Triton X-100 and Tween-20 were used at concentrations of 0.2% and 2%, respectively, due to their inhibitory effect on P. aeruginosa growth at higher concentrations.

The relative hydrophobicity of each molecule was determined by measuring its contact angle using a contact angle goniometer. This device measures the angle that a droplet makes with the surface and gives a quantitative measure of hydrophobicity (Figure 4B). We formed droplets on an oleophobic surface to maintain droplet forms for a wide range of hydrophobicities using a single surface. PDMS had the smallest contact angle, indicating the greatest hydrophobicity of the molecules tested (Figure 4C). In order of decreasing hydrophobicity (increasing contact angle) were PDMS, the fatty acids, triglycerides, and the surfactant Tween-20 (Figure 4C and Figure S5 in Supplementary Materials). Water was included as a reference and had the greatest contact angle. We spotted 6 µL of each molecule at satellite positions in swarm interaction assays. In support of our hypothesis, PDMS and the fatty acids repelled tendrils (Figure 4D). Triglycerides and surfactants, which are less hydrophobic, did not repel tendrils (Figure 4D). In addition, we tested the effect of viscosity on tendril repulsion by using PDMS at two different viscosities, 200 cSt and 1000 cSt. For reference, water has a viscosity of 1 cSt at 20°C. The lower viscosity (200 cSt) PDMS produced greater repulsion than the higher viscosity PDMS (1000 cSt) (Figure 4D and S5B in Supplementary Materials), suggesting that a lower viscosity molecule of the same hydrophobicity produces greater repulsion. Together, these results show that hydrophobic molecules repel swarm tendrils.

We next hypothesized that hydrophobic molecules repel tendrils by altering the spatial organization of surfactant that is produced by the swarm. The surfactant contains RLs and precursor 3-(3-hydroxyalkanoyloxy) alkanoic acids (HAAs), which facilitate tendril development (Caiazza et al., 2005; Soberón-Chávez et al., 2005; Abdel-Mawgoud et al., 2010). Tracking the spatial distribution of surfactants has been a significant challenge because they are optically transparent. Surfactants from swarms have been visualized using methylene blue and Nile red dyes (Siegmund and Wagner, 1991; Morris et al., 2011) but these dyes can alter surfactant and swarm dynamics. To better measure the dynamics of surfactants and swarm tendrils without perturbing them, we used the recently-developed imaging method IRIS, which requires no modification of growth conditions or staining (Kasallis et al., 2023). IRIS applies the principle of deflectometry to illuminate swarm surfaces by projecting a structured image onto the surface and capturing the reflection (Figure S6A). The structured image increases the contrast of surface features, most notably the liquid-air interface at the edge of the swarm. Conventional ambient lighting and reflection of a non-structured image capture the boundaries of tendrils but do not differentiate the surfactant from the surrounding surface (Figure 5A). In contrast, IRIS imaging reveals high resolution details about the swarm. Notably, the tendrils grow on the surface of an optically transparent liquid-like zone (Figure 5A). We used edge detection to further demarcate the tendrils and the boundary of the liquid-like zone, resulting in masked IRIS images (Figure 5A). The liquid-like zone is not present in the ΔrhlAB mutant, which does not produce the HAAs or RLs (Figure S6B in Supplementary Materials). This observation suggests that the liquid-like zone is composed of HAAs and RLs.

FIGURE 5
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Figure 5 Role of the P. aeruginosa surfactant layer in swarm repulsion. (A) Swarm assay in which WT P. aeruginosa was spotted at the center and imaged using ambient lighting, a reflected uniform image, or a reflected structured image at 11 hours following inoculation. The enlarged portion of the IRIS Image shows tendril boundaries and surfactant boundary. The masked IRIS image indicates the surfactant layer (yellow) and P. aeruginosa (green) boundaries. The differential IRIS image indicates components of the surfactant layer and P. aeruginosa that are dynamic. (B) IRIS, masked IRIS, and differential IRIS images of swarm interaction assays in which WT P. aeruginosa was spotted at the center and either test molecules (1000 cSt PDMS or water) or strains (TOB-treated P. aeruginosa or P. aeruginosa ΔrhlAB) were spotted at satellite positions. Images were acquired 12-14 hours following inoculation. Masked IRIS images indicate the surfactant layer (yellow), P. aeruginosa (green), and test molecules or strains at the satellite positions (red). In ΔrhlAB, the surfactant layer overlaps the ΔrhlAB satellite colonies. Dashed lines at satellite positions show the boundary of the initial spot. Magnified raw IRIS images that are not masked can be found in Figure S6C in the Supplementary Materials.

Recent work using shadowgraphy and optical profilometry revealed that RLs penetrate the agar layer, causing the agar to swell (Deforet, 2023). This effect alters the surface of the agar such that the RL-swelled agar is raised relative to the surrounding agar surface and P. aeruginosa is present above the RL-swelled agar ((Deforet, 2023) and Figure 5A). The liquid-like zone observed using IRIS is consistent with the penetration of RLs throughout the agar and the presence of RLs at the surface of the agar. It is unclear whether RLs at the surface are suspended in water, are present as a slurry of RL with agar, or are contained within the swelled agar. We refer to the liquid-like zone observed here as the surfactant layer because it requires HAAs and RLs and appears below the tendrils. We performed differential analysis of timelapse IRIS images to further demarcate tendril and surfactant layer boundaries and identify the components of the swarm that were dynamic (Figure 5A). A previous analysis showed that the surfactant layer expands in lockstep with the tendril edges, which suggests that movement of the surfactant layer and tendrils are coupled (Kasallis et al., 2023). Building upon this finding, we considered the possibility that hydrophobic molecules could alter the flow of the surfactant layer, which in turn could alter the direction of tendrils.

We imaged the dynamics of the P. aeruginosa surfactant layer and its interaction with the hydrophobic molecules using IRIS timelapse imaging. PDMS, which was the most hydrophobic molecule that we tested (Figure 4C), strongly deflected the surfactant layer, causing the surfactant to flow clearly around the PDMS (Figure 5B, and Figure S6C and Movie S2 in Supplementary Materials). Concomitant with the deflection of the surfactant, the tendrils moved around the PDMS. A notable feature of the dynamics is that the tendrils were constrained to move within the boundary of the surfactant layer. These observations raise the possibility that the surfactant layer sets the boundaries on which the tendrils can move, which constrains and alters the tendril movements. It is unlikely that P. aeruginosa could alter the direction of the surfactant flow to cause the surfactant to move around the PDMS. A more plausible explanation is that the hydrophobicity of the PDMS deflects the surfactant layer, causing the surfactant to move around the PDMS. Consistent with this, neither the surfactant layer nor tendrils were deflected by water (Figure 5B and Figure S6C in Supplementary Materials). These results suggest that the physicochemical interaction between the surfactant layer and PDMS is responsible for tendril repulsion by PDMS, though the interpretation does not entirely rule out a role for sensing of PDMS by P. aeruginosa. In support of the physicochemical interpretation, oleic acid and linoleic acid formed large boundaries that could contribute to the deflection of the surfactant layer (Figure S6D).

We hypothesized that stressed P. aeruginosa populations (Figures 1C, D) repel tendrils through surfactant deflection. In support of this hypothesis, surfactant from the P. aeruginosa swarm was deflected by TOB-treated P. aeruginosa satellite populations (Figure 5B). The movement of swarm tendrils away from TOB-treated populations was concomitant with the deflection of the surfactant (Movie S3 in Supplementary Materials). These data suggest that tendril repulsion by antibiotic-stressed P. aeruginosa is caused by deflection of the surfactant layer, which in turn, alters the movement of swarm tendrils around the stressed P. aeruginosa population. We verified that P. aeruginosa alone does not repel tendrils or deflect the surfactant layer by using the ΔrhlAB strain, which is deficient in RL production and in these growth conditions produces insufficient PQS to repel tendrils without antibiotic stress (Bru et al., 2019). Surfactant produced by the WT swarm first merged with ΔrhlAB colonies at the satellite positions, followed by the advancement of swarm tendrils into the satellite boundaries (Figure 5B and Figure S6C in the Supplementary Materials).

The effects on the surfactant layer were prominent in differential IRIS images, which show that the surfactant boundary formed a concave feature as it approached PDMS or TOB-treated P. aeruginosa (Figure 5B). In contrast, no concave features were observed in the surfactant layer as it approached the ΔrhlAB mutant colony. In addition, the surfactant was attracted to the ΔrhlAB colony, causing the surfactant layer to develop into a hexagonal pattern (Figure 5B and Movie S4 in Supplementary Materials). We note that surfactant flow from the WT swarm is observed within the boundary of the ΔrhlAB satellite colony, as detected by the differential IRIS images. This flow is not observed in the TOB-treated WT colonies (Movie S3 in Supplementary Materials) and is consistent with merging of the surfactant layer with the ΔrhlAB colony.

We reasoned that PQS could deflect the surfactant layer from approaching tendrils, based on the observation that PQS repels swarm tendrils ((Bru et al., 2019) and Figure S2B). Indeed, IRIS imaging showed that PQS deflects the surfactant layer, causing a simultaneous change in tendril direction (Figure 6A and Movie S5 in Supplementary Materials). Dimethyl sulfoxide (DMSO), the solvent in which PQS was dissolved, did not deflect the surfactant layer (Figure S7A in Supplementary Materials). Concave features were observed in the surfactant layer in the differential IRIS image, consistent with the surfactant deflection that was observed with PDMS and TOB-treated P. aeruginosa (Figure 5B). We measured the magnitude of surfactant deflection by PQS through image analysis (see Methods section and Figure S7B in Supplementary Materials) and found that the surfactant deflection area and tendril repulsion radius both increased with increasing concentrations of PQS (r = 0.88) (Figure 6B). This finding supports the hypothesis that PQS repels tendrils physically by disrupting the surfactant layer flow. Together, these data suggest that tendril repulsion by stressed P. aeruginosa populations is caused by physicochemical interactions between PQS and the surfactant layer. These interactions in turn alter the surfactant flow and tendril direction around stressed P. aeruginosa populations. We note that while surfactant layer deflection has a significant role in tendril repulsion, the data do not rule out a role for sensing of PQS by the P. aeruginosa cells within the tendrils. It is possible that PQS sensing could affect the surfactant production or composition that could indirectly affect tendril direction.

FIGURE 6
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Figure 6 PQS increases both surfactant deflection and tendril repulsion. (A) IRIS, masked IRIS, and differential IRIS images of a swarm interaction assay in which WT P. aeruginosa was spotted at the center and a range of PQS concentrations (indicated in mM) was spotted at satellite positions. Dashed lines in the IRIS image indicate the boundaries of the initial spots. Masked IRIS images indicate surfactant layer (yellow), P. aeruginosa (green), and initial PQS spots (red). The differential IRIS image indicates components of the surfactant layer and P. aeruginosa that are dynamic. Magnified raw IRIS images that are not masked are shown in Figure S6C in the Supplementary Materials. (B) Tendril repulsion radii and surfactant deflection areas for the range of PQS concentrations from three independent experiments. The correlation coefficient (r value) and a least squares fit (dashed line) are displayed on the plot. *** denotes p < 0.001 computed using a two-tailed t-test. Repulsion radius was measured as the distance from the nearest tendril to the center of the PQS spot, regardless of whether the tendril contacted the boundary of the initial spot. Images were acquired 14.5-17 hours following inoculation.

S. aureus repels P. aeruginosa tendrils by deflecting the surfactant layer

We rationalized that the repulsion of tendrils by S. aureus could be caused by a similar repulsion mechanism observed by PDMS, TOB-treated P. aeruginosa, and PQS. In particular, the amphipathic property of the PSM fibrils produced by S. aureus could deflect the P. aeruginosa surfactant layer, thereby altering tendril direction. IRIS imaging revealed that the P. aeruginosa surfactant layer was indeed deflected and moved around WT S. aureus colonies (Figures 7A, B, Figure S6C and Movie S6 in Supplementary Materials). Surfactant deflection was significantly reduced in Δpsmα and Δpsmα Δhld mutants (Figures 7A, B), which are defective in the production of PSMs and do not repel tendrils. These data suggest that PSMs produced by S. aureus deflect the surfactant layer, which results in tendril repulsion.

FIGURE 7
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Figure 7 Surfactant layer deflection is diminished in PSM mutants. (A) IRIS, masked IRIS, and differential IRIS images of swarm interaction assays in which WT P. aeruginosa was spotted at the center and WT S. aureus or PSM mutants were spotted at satellite positions. The masked IRIS images indicate the surfactant layer (yellow), P. aeruginosa (green), and initial spots (red). Differential IRIS images indicate components of the surfactant layer and P. aeruginosa that are dynamic. Magnified raw IRIS images that are not masked are shown in Figure S6C in the Supplementary Materials. (B) Tendril repulsion radii (black dots) and surfactant deflection area (blue dots) of WT S. aureus and PSM mutants. The dots represent the average radius or area from six satellite positions on a single plate, red bars indicate the average of three independent experiments, and error bars indicate standard deviation. T-tests were performed as two-tailed distributions with unequal variance. *** denotes p < 0.001. (C) IRIS images of the fluidic boundary produced by S. aureus USA300 s1 cultured on M8 or LB medium containing 0.5% agar. The fluidic boundary is outlined in the lower images (red dashed line). Scale bar represents 3 mm. Images were acquired 14 hours following inoculation.

Additional support for surfactant deflection by S. aureus is the observation of a fluidic boundary that moves outwards from WT S. aureus colonies during growth. The fluidic boundary surrounds the colony and extends 3 mm beyond the colony edge (Figure 7C and Movie S6 in Supplementary materials). While the composition of the fluidic boundary is unclear, we observed amyloid fibrils at a comparable distance (1.5 to 3 mm) from the S. aureus colony using TEM (Figure 3B). The observation of the fluidic boundary at a comparable distance as the fibrils offers a potential explanation for how the fibrils could be transported away from the colony edge. The fibrils are absent at this distance in the Δpsmα Δhld mutant (Figure 3B), which is consistent with the model that PSM fibrils mediate the deflection of the P. aeruginosa surfactant layer. We note that concave features in the surfactant layer were observed in the differential IRIS images near WT S. aureus colonies (Figure 7A). The features are similar to the concave features produced by other tendril-repelling molecules (Figures 5B, 6A). The size of the concave features is diminished in the Δpsmα Δhld mutant compared to WT, though they are not entirely abolished (Figure 7A). Together, these data suggest that PSM amyloid fibrils deflect the P. aeruginosa surfactant layer, resulting in the repulsion of P. aeruginosa tendrils. Because surfactant deflection is not completely abolished by the Δpsmα Δhld mutant, this suggests that other molecules produced by S. aureus, including PSMβ could contribute to surfactant deflection. The deflection of surfactant by S. aureus is consistent with the mechanism of tendril repulsion observed by PDMS, TOB-treated P. aeruginosa, and PQS (Figures 5B, 6A).

Repulsion by surfactant produced by other organisms

Our data suggests that molecules that impact the surfactant layer could alter tendril movement. We investigated the potential for surfactants produced by other species to alter tendril patterns. We rationalized that such surfactants would alter the flow of the P. aeruginosa surfactant layer. We assessed the impact of surfactin, which is a surfactant that is produced by B. subtilis, and of porcine lung surfactant. Consistent with our model, surfactin repelled P. aeruginosa swarm tendrils (Figure 8A). The effect of lung surfactant on tendrils was dependent on concentration. Lung surfactant at 0.25 mg/mL repelled tendrils but at a higher concentration of 1.6 mg/mL, inhibited P. aeruginosa swarm expansion altogether (Figure 8B and Figure S7C in Supplementary Materials). Consistent with the surfactant deflection model, lung surfactant produced a fluidic boundary that expanded radially from the initial spot (Figure 8B and Figure S7C in Supplementary Materials), similar to the boundaries produced by oleic acid and linoleic acid (Figure S6D). These results suggest that interactions between the surfactant layer and surfactant molecules have a significant impact on the organization of P. aeruginosa swarms.

FIGURE 8
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Figure 8 P. aeruginosa repulsion mediated by the surfactant layer. (A) Swarm interaction assays in which WT P. aeruginosa was spotted at the center and 10 µM surfactin from B. subtilis was spotted at satellite positions. Images were acquired 16.5 hours following inoculation. (B) Swarm interaction assays and masked IRIS images in which WT P. aeruginosa was spotted at the center and 6 μL of lung surfactant at the indicated concentrations was spotted at satellite positions. The masked IRIS images indicate the initial inoculum of lung surfactant (red), the fluidic boundary that expands from the lung surfactant (pink), P. aeruginosa surfactant (yellow), and P. aeruginosa (green). Images of the interaction assays and IRIS images were acquired 18 and 3 hours following inoculation, respectively. The raw IRIS images that do not contain masks are in Figure S7C in the Supplementary Materials. Dashed lines in (A, B) indicate the boundaries of the initial inoculum. (C) Schematic of the proposed P. aeruginosa swarm repulsion model. PSMs surround the S. aureus and deflect the P. aeruginosa surfactant layer, thereby causing tendril repulsion.

Discussion

A fundamental aspect of multispecies bacterial communities is how one organism interacts with another. Here, we have investigated the spatial interaction between two competing opportunistic pathogens of major clinical importance, P. aeruginosa and S. aureus. Swarms of P. aeruginosa were repelled by S. aureus, which created a cell-free physical barrier and facilitated coexistence between the two species. We found that physicochemical interactions between the surfactant produced by P. aeruginosa and S. aureus PSMs create the separation. Our results provide insight into P. aeruginosaS. aureus interactions and into P. aeruginosa swarming. The findings have important implications on the understanding of how bacteria interact with other species and host environments.

Differential responses to antibiotic treatment

Our survey of clinical isolates revealed that under TOB-induced stress, P. aeruginosa and S. aureus have different effects on approaching P. aeruginosa swarms. Almost all of the TOB-treated P. aeruginosa isolates repelled P. aeruginosa swarms (Figures 1E, F). The converse was true in S. aureus strains. While almost all untreated clinical S. aureus strains repelled P. aeruginosa swarms, TOB treatment of these strains abolished the repulsive effect (Figures 2D, E). This suggests that the antibiotic stress response of P. aeruginosa induces production of the signaling molecule PQS. However, an analogous stress response in which S. aureus produces additional signaling molecules that repel P. aeruginosa was not observed.

Repulsion of P. aeruginosa by S. aureus

The combination of IRIS and TEM imaging revealed a novel P. aeruginosa - S. aureus interaction. IRIS images showed that S. aureus colonies produce a fluidic boundary that expands radially from the colony, forming a ring up to 3 mm beyond the colony’s edge after 10 hours of growth. The fluidic boundary is not visible under ambient lighting or with conventional imaging methods that use non-reflective illumination (Figure 5A). TEM showed that PSM-dependent fibrils lie within the fluidic boundary region, up to 3 mm away from the colony’s edge (Figure 3B). The combination of IRIS and TEM data raises a number of questions regarding how fibrils are formed and transported. It is possible that PSMs could be carried by a fluid and subsequently nucleate into fibrils during transport away from the colony, or that already-formed fibrils could be carried away by a fluid. We point out that the fluidic boundary produced by S. aureus is not limited to the growth conditions on swarm media plates here. In particular, a fluidic boundary is also observed around S. aureus colonies that are cultured on LB plates (Figure 7C), which are used widely to culture this organism.

We consider how PSMs deflect the P. aeruginosa surfactant layer. The surfactant consists of RLs and HAAs, which contain long chain hydrophobic domains. It would be expected that the surfactant layer of P. aeruginosa would merge with molecules of similar properties, such as the amphiphilic molecule PQS. In fact, RLs solubilize PQS (Calfee et al., 2005). Surprisingly, the surfactant layer is repelled by PQS (Figure 6A). One possibility of how an amphiphilic molecule could repel the surfactant layer is that an intermediate layer of water provides a physical barrier between the surfactant layer and amphiphilic molecule. In the case of PSMs, which are amphipathic, a water layer could form between the surfactant and PSM fibrils, which would deflect the surfactant and produce a cell-free zone of repulsion between S. aureus and P. aeruginosa (Figure 8C). An additional factor that could affect surfactant deflection is viscosity. We found that PDMS of lower viscosity (200 cSt) repelled tendrils to a greater extent than higher viscosity (1000 cSt) PDMS (p=0.02) (Figure S5B in Supplementary Materials). Thus, a change in the viscosity of the area outside of the S. aureus colony produced by PSMs could contribute to surfactant layer deflection. In addition, small differentials in the amphipathicity between the surfactant layer and PSMs could also contribute to deflection. Finally, S. aureus may produce additional molecules that disrupt the surfactant layer that have not been identified in this study.

Previous investigations of P. aeruginosa - S. aureus interactions suggest that P. aeruginosa is predisposed to interact with S. aureus if the two species are within close proximity (microns) of one another (Limoli et al., 2019). During chronic infections, P. aeruginosa and S. aureus can evolve a mutualistic relationship in which P. aeruginosa does not inhibit S. aureus growth, but rather the interaction enhances P. aeruginosa growth (Michelsen et al., 2014; Frydenlund Michelsen et al., 2016). In contrast, our study shows that at the longer (millimeter) scale, S. aureus repels P. aeruginosa and the two species remain isolated, promoting species heterogeneity. In particular, RLs interact with the S. aureus membrane, making it more permeable to TOB, and thus sensitize S. aureus to TOB-mediated killing (Yarlagadda and Wright, 2019). The separation mechanism described here creates a physical barrier that reduces the likelihood that RLs reach S. aureus at the millimeter scale.

Role of surfactant flow in swarm organization

Our analysis of P. aeruginosa swarming suggests that fluid mechanics has a significant role in tendril formation. In particular, the surfactant and tendrils form two separate layers that are coupled in motion in which the former is required for the movement of the latter. The surfactant layer constrains the movement of the swarm tendrils such that P. aeruginosa cannot move beyond the surfactant layer boundary. The movement of the surfactant layer around repulsive molecules such as PQS and PSMs thus directs the motion of the tendrils to move around these molecules. Recent work using shadowgraphy shows that RLs penetrate and flow through the agar layer (Deforet, 2023). Our observation of the surfactant flow using IRIS is consistent with movement of RLs through the agar layer. The details of how repulsive molecules affect RL flow within the agar layer are unclear. It is possible that such molecules repel the movement of RLs within the agar or that alternatively, only RLs near the agar surface are affected. In particular, the fluidic boundaries observed moving away from S. aureus, oleic acid, linoleic acid, and lung surfactants are consistent with the penetration and movement of these molecules through the agar layer as well. The details of surfactant flow in three dimensions will need to be considered in future studies to address this issue. The mechanism of surfactant disruption has important implications for understanding how tendrils move on surfaces and are repelled or merge with other tendrils, such as is observed with the ΔsadB and ΔrhlC mutants (Caiazza et al., 2005). The flow of surfactant may need to be considered in such cases and in other aspects of swarm development.

While our results suggest an important role of the surfactant layer in tendril development, they do not preclude a role for sensing. Multiple P. aeruginosa sensors can explain the detection of molecules such as RLs and PQS by swarm tendrils. For example, it is possible that sensing of PSMs and PQS could alter the motion of tendrils around S. aureus and TOB-treated P. aeruginosa, respectively. A mechanism that explains how the tendril motion is altered in response to sensing is lacking, however. Metabolic sensing of hydrocarbon molecules provides an additional possible mechanism that could alter tendril development (Kang et al., 2010). In addition, we note that coupling between the surfactant and tendril layers is likely not the only mechanism that directs tendril movement. For example, after the surfactant layer reaches the boundary of the Petri dish, tendrils continue to move, although at a slower rate. Continued tendril growth in which the surfactant and tendril layers are decoupled may be possible. Biophysical models of pressure-driven flow and Marangoni flow (Fauvart et al., 2012; Yang et al., 2017) may explain the continued expansion of swarm tendrils in the absence of surfactant flow.

Potential role of surfactant interactions in coexistence

In infection settings, P. aeruginosa and S. aureus frequently colonize the same environment. Here, it may be mutually beneficial for both species to remain spatially separated. The interactions between P. aeruginosa and other organisms may be affected by the presence of surfactant. Similarly, interactions between P. aeruginosa and S. aureus may be affected by PSM production. Consistent with surfactant and PSMs having important roles in infection, significant levels of RLs have been measured in the lungs of cystic fibrosis patients (Kownatzki et al., 1987). Likewise, PSMs have a major role in lung infection models of S. aureus (Bloes et al., 2017). The repulsive interactions observed in this study could facilitate the coexistence of the two species in different micro-niches during coinfection. In particular, PSM fibrils could reduce RL-induced aminoglycoside sensitivity. As the role of swarming in infection is unclear, future experiments will need to address the role of surfactant interactions with PSMs in vivo.

The finding that the P. aeruginosa surfactant layer has a major role in swarm organization may have implications on P. aeruginosa colonization in natural and host environments. The findings suggest that molecules that interact with P. aeruginosa surfactants can impact the spatial organization of the population. Moreover, the physicochemical nature of this interaction suggests that chemically-diverse hydrophobic molecules could alter this spatial organization. In support of this claim, surfactin from B. subtilis repelled P. aeruginosa swarms. This observation is remarkable because B. subtilis is evolutionarily distant from P. aeruginosa yet causes repulsion of P. aeruginosa tendrils. In host environments such as the human lung, cells produce a number of surfactants (Han and Mallampalli, 2015) that could impact colonization or swarm-dependent spreading of P. aeruginosa during infection. Our results show that lung surfactant repelled P. aeruginosa tendrils and at a sufficiently high concentration, inhibited swarming and the proliferation of P. aeruginosa. In contrast, the lack of surfactant facilitated the expansion of P. aeruginosa. These findings are consistent with the finding that deficiencies in surfactant production promote susceptibility to P. aeruginosa colonization. Indeed, cystic fibrosis patients, who are deficient in lung surfactant production, and mice that are deficient in surfactant production have increased susceptibility to P. aeruginosa colonization (Griese et al., 1997; Glasser et al., 2008). Future studies should address the extent that surfactant interactions can alter the expansion of P. aeruginosa in vivo. Given that numerous bacterial species found in natural and host environments produce unique surfactants, our findings raise the possibility that surfactants produced among different species of bacteria in polymicrobial environments could have a significant impact on bacterial spatial organization and dissemination, and on disease development.

Materials and methods

Growth conditions and materials

Strains were streaked from frozen stocks onto LB Broth-Miller (BD, Franklin Lakes, NJ) plates containing 2% Bacto agar (BD) and incubated overnight at 37°C. Single colonies were inoculated into the same broth and incubated 16 to 18 hours in a roller drum at 20 rpm and 37°C. All strains used in this study are described in Table S1 in the Supplementary Materials. Clinical isolates were obtained from the Whiteson Lab and UCI Health Medical Microbiology Laboratory; no identifying information was collected and therefore IRB approval was not required. The clinical strains used in the study were all of airway and skin wound isolates that were available to us.

Compounds in swarm interaction assays including 200 cSt PDMS, 1000 cSt PDMS, oleic acid, linoleic acid, glyceryl trioleate, glyceryl trilinoleate, Triton X-100, Tween-20, 2-heptyl-3-hydroxy-4-quinolone (PQS), and surfactin isolated from Bacillus subtilis (Cat. #S3523), were obtained from Sigma Aldrich (St. Louis, MO) and were used in their pure concentrated forms unless otherwise specified. PQS was dissolved in dimethylsulfoxide (DMSO). Strains at satellite positions were treated with antibiotics using tobramycin (Thermo Fisher Scientific Hampton, NH) at a concentration of 0.5 mg/mL. The porcine pulmonary surfactant extract Curosurf (poractant alfa) (Chiesi USA, Inc., Cary, NC) was obtained at a concentration of 80 mg/mL, diluted in water, and used at the indicated concentrations.

Swarm interaction assays

Assays were performed as described previously (Bru et al., 2019; Bru et al., 2020). Briefly, 100 x 15mm Petri dishes contained 20 mL of M8 minimum medium (Bru et al., 2020) supplemented with 1 mM MgSO4, 0.2% glucose, 0.5% Casamino Acids (BD), and 0.5% Bacto agar (BD). Following sterilization, media in Petri dishes solidified for 1 hour with lids on at room temperature, and then dried for 30 minutes without lids in a laminar flow hood at 300 cubic ft./min. with approximately 40 to 50% ambient humidity. Strains were cultured 16 to 18 hours in LB broth. WT P. aeruginosa was spotted at the center of the swarming plate in a 5 µL droplet. Strains or compounds were spotted at satellite positions using 6 µL droplets at 6 satellite positions that were equidistant from each other along a 5.8 cm-diameter circle centered at the swarming plate (Figure S7D in the Supplementary Materials). Plates were incubated overnight at 37°C with a darkened Petri dish lid. Images were acquired every 30 minutes for 18 to 20 hours using an Epson scanner (Epson, Los Alamitos, CA) that was controlled using RoboTask (robotask.com) and then processed using ImageJ 1.54d (NIH, Bethesda, MD). For the antibiotic treatment of strains at satellite positions, tobramycin (Thermo Fisher Scientific) was mixed with the bacterial inoculum to a final concentration of 0.5 mg/mL and the mixture was spotted immediately onto swarming plates in 6 µL droplets. The repulsion radius at each satellite colony was measured as the distance from the center of the satellite to the nearest tendril along a line that is tangent to the 5.8 cm-diameter circle that is concentric with the swarming plate (Figure S7D in the Supplementary Materials). If a tendril contacted the boundary of the initial satellite spot, the repulsion radius was marked as zero, except for the measurements to determine the correlation between repulsion radius and surfactant deflection area by PQS (Figure 6B).

Transmission electron microscopy

Mesh copper grids that were 3 mm in diameter (Electron Microscopy Sciences, Hatfield, PA) were placed with the carbon layer facing up at or adjacent to inoculation positions on swarming plates. Six microliters of S. aureus that had been cultured in LB broth for 16 to 18 hours was spotted onto the inoculum position. Plates containing the inoculums and copper grids were incubated at 37°C and 50% humidity for 16 to 18 hours. The grids were stained using 1% uranyl acetate (Electron Microscopy Sciences), dried overnight at room temperature, and imaged using a JEM-2800 High Throughput Electron Microscope (JEOL, Akishima, Japan) using an acceleration voltage of 200 kV.

Contact angle measurements

Test compounds were pipetted vertically in one microliter droplets at room temperature onto a Notak oleophobic-coated (SilcoTek, Bellefonte, PA) polished stainless steel sheet surface and imaged immediately for 10 seconds using a Model 90 goniometer (ramé-hart, Succasunna, NJ) with a Summit SK2-3.1X 3.1MP digital camera (OptixCam, Roanoke, VA). Contact angles were measured from timelapses at the 7 second timepoint after the initial droplet placement onto the surface using ImageJ and the sessile drop method. Angles were measured for the left and right sides of the droplet in the images and averaged.

Surface growth quantification

The growth of S. aureus on swarm plates (Figure S3A in Supplementary Materials) was determined by acquiring images as described in the swarm interaction assays. Surface growth intensities were computed using ImageJ by measuring the pixel intensities of S. aureus colonies along a ring that corresponded to S. aureus growth along the outer radius of the colony. Pixel intensities of S. aureus were subtracted by those outside of the S. aureus colony, arriving at a surface growth intensity. Intensities were normalized by dividing by the value measured within 1 hour of inoculation for each strain.

IRIS imaging

Imaging was performed as described previously (Kasallis et al., 2023). Briefly, swarming plate surfaces were illuminated by an ASUS LCD VE278Q monitor (ASUS, Fremont, CA) that displayed a structured image that consisted of evenly-spaced white squares in a 17 x 31 grid pattern on a black background (Figure S6A in Supplementary Materials). The reflection of the structured image on the swarming plate surface was acquired using a Canon EOS Rebel T7 DSLR (Canon, Melville, NY) with a Canon EF-S 18-55 mm lens. The inner surfaces of Petri dishes were scratched with a metal wire brush to reduce reflections. Swarming plates were incubated at 37˚C and 50% humidity. For timelapses, the lid was removed and replaced at regular intervals using a robotic arm to allow for image acquisition. Masks of the IRIS images were constructed by passing the magnetic lasso tool in Adobe Photoshop (Adobe, San Jose, California) over edge features in IRIS images to identify boundaries. Zones that were enclosed within the boundaries were designated as P. aeruginosa or surfactant. Differential images were constructed using the imabsdiff function in Matlab version R2017a (Mathworks, Natick, MA) using images that were acquired at 5 minute (Figure 5A) or 30 minute intervals (all others). The surfactant deflection area was defined as the total area in the vicinity of the satellite spot that did not contain P. aeruginosa surfactant (Figure S7B in the Supplementary Materials). Our identification of the P. aeruginosa surfactant layer boundary was aided by following surfactant flow in timelapse IRIS movies and using differential IRIS images. An additional boundary of the deflection area was defined as an arc that connected two of the nearest surfactant layer boundaries that were not deflected.

Data availability statement

The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

Author contributions

AS, J-LB, and QZ developed the IRIS device. J-LB, SJK, KW, NMH-K, EW, DHL, and AS designed experiments and determined project direction. J-LB, SJK, RC, QZ, PP, and JN performed experiments and analyzed data. J-LB, SJK, and AS drafted and edited the manuscript. All authors contributed to the article and approved the submitted version.

Acknowledgments

We thank Joshua Shrout for helpful discussions about swarming and Cassiana Bittencourt (UCI Health), Zachary Lu, Joann Phan and Tara Gallagher (Whiteson Lab) for collecting the clinical isolates. NMH-K was supported by a grant from the Independent Research Fund Denmark (1054-00099B) and AS was supported by grants from the NIH NIAID (R56AI163196 and R01AI163196).

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Supplementary material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2023.1245874/full#supplementary-material

References

Abdel-Mawgoud, A. M., Lépine, F., Déziel, E. (2010). Rhamnolipids: diversity of structures, microbial origins and roles. Appl. Microbiol. Biotechnol. 86, 1323–1336. doi: 10.1007/s00253-010-2498-2

PubMed Abstract | CrossRef Full Text | Google Scholar

Beal, R., Betts, W. B. (2000). Role of rhamnolipid biosurfactants in the uptake and mineralization of hexadecane in Pseudomonas aeruginosa. J. Appl. Microbiol. 89, 158–168. doi: 10.1046/j.1365-2672.2000.01104.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Benincasa, M., Abalos, A., Oliveira, I., Manresa, A. (2004). Chemical structure, surface properties and biological activities of the biosurfactant produced by Pseudomonas aeruginosa LBI from soapstock. Antonie Van Leeuwenhoek 85, 1–8. doi: 10.1023/B:ANTO.0000020148.45523.41

PubMed Abstract | CrossRef Full Text | Google Scholar

Biswas, L., Biswas, R., Schlag, M., Bertram, R., Götz, F. (2009). Small-colony variant selection as a survival strategy for Staphylococcus aureus in the presence of Pseudomonas aeruginosa. Appl. Environ. Microbiol. 75, 6910–6912. doi: 10.1128/AEM.01211-09

PubMed Abstract | CrossRef Full Text | Google Scholar

Bloes, D. A., Haasbach, E., Hartmayer, C., Hertlein, T., Klingel, K., Kretschmer, D., et al. (2017). Phenol-soluble modulin peptides contribute to influenza A virus-associated staphylococcus aureus pneumonia. Infect. Immun. 85, e00620–e00617. doi: 10.1128/IAI.00620-17

CrossRef Full Text | Google Scholar

Bru, J.-L., Rawson, B., Trinh, C., Whiteson, K., Høyland-Kroghsbo, N. M., Siryaporn, A. (2019). PQS produced by the pseudomonas aeruginosa stress response repels swarms away from bacteriophage and antibiotics. J. Bacteriol 201, e00383–e00319. doi: 10.1128/JB.00383-19

PubMed Abstract | CrossRef Full Text | Google Scholar

Bru, J.-L., Siryaporn, A., Høyland-Kroghsbo, N. M. (2020). Time-lapse imaging of bacterial swarms and the collective stress response. J. Vis. Exp., (159), e60915. doi: 10.3791/60915

CrossRef Full Text | Google Scholar

Caiazza, N. C., Shanks, R. M. Q., O’Toole, G. A. (2005). Rhamnolipids modulate swarming motility patterns of Pseudomonas aeruginosa. J. Bacteriol, 187, 7351–7361. doi: 10.1128/JB.187.21.7351-7361.2005

PubMed Abstract | CrossRef Full Text | Google Scholar

Calfee, M. W., Shelton, J. G., McCubrey, J. A., Pesci, E. C. (2005). Solubility and bioactivity of the Pseudomonas quinolone signal are increased by a Pseudomonas aeruginosa-produced surfactant. Infect. Immun. 73, 878–882. doi: 10.1128/IAI.73.2.878-882.2005

PubMed Abstract | CrossRef Full Text | Google Scholar

Camus, L., Briaud, P., Vandenesch, F., Doléans-Jordheim, A., Moreau, K. (2022). Mixed Populations and Co-Infection: Pseudomonas aeruginosa and Staphylococcus aureus. Adv. Exp. Med. Biol. 1386, 397–424. doi: 10.1007/978-3-031-08491-1_15

PubMed Abstract | CrossRef Full Text | Google Scholar

Cogen, A. L., Yamasaki, K., Sanchez, K. M., Dorschner, R. A., Lai, Y., MacLeod, D. T., et al. (2010). Selective antimicrobial action is provided by phenol-soluble modulins derived from Staphylococcus epidermidis, a normal resident of the skin. J. Invest. Dermatol. 130, 192–200. doi: 10.1038/jid.2009.243

PubMed Abstract | CrossRef Full Text | Google Scholar

Collier, D. N., Anderson, L., McKnight, S. L., Noah, T. L., Knowles, M., Boucher, R., et al. (2002). A bacterial cell to cell signal in the lungs of cystic fibrosis patients. FEMS Microbiol. Lett. 215, 41–46. doi: 10.1111/j.1574-6968.2002.tb11367.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Deforet, M. (2023). Long-range alteration of the physical environment mediates cooperation between Pseudomonas aeruginosa swarming colonies. Environ. Microbiol. 25(8), 1451–1464. doi: 10.1111/1462-2920.16373

PubMed Abstract | CrossRef Full Text | Google Scholar

Fauvart, M., Phillips, P., Bachaspatimayum, D., Verstraeten, N., Fransaer, J., Michiels, J., et al. (2012). Surface tension gradient control of bacterial swarming in colonies of Pseudomonas aeruginosa. Soft Matter 8, 70–76. doi: 10.1039/C1SM06002C

CrossRef Full Text | Google Scholar

Frydenlund Michelsen, C., Hossein Khademi, S. M., Krogh Johansen, H., Ingmer, H., Dorrestein, P. C., Jelsbak, L. (2016). Evolution of metabolic divergence in Pseudomonas aeruginosa during long-term infection facilitates a proto-cooperative interspecies interaction. ISME J. 10, 1323–1336. doi: 10.1038/ismej.2015.220

PubMed Abstract | CrossRef Full Text | Google Scholar

Gdaniec, B. G., Bonini, F., Prodon, F., Braschler, T., Köhler, T., van Delden, C. (2022). Pseudomonas aeruginosa rhamnolipid micelles deliver toxic metabolites and antibiotics into Staphylococcus aureus. iScience 25, 103669. doi: 10.1016/j.isci.2021.103669

PubMed Abstract | CrossRef Full Text | Google Scholar

Glasser, S. W., Senft, A. P., Whitsett, J. A., Maxfield, M. D., Ross, G. F., Richardson, T. R., et al. (2008). Macrophage dysfunction and susceptibility to pulmonary Pseudomonas aeruginosa infection in surfactant protein C-deficient mice. J. Immunol. 181, 621–628. doi: 10.4049/jimmunol.181.1.621

PubMed Abstract | CrossRef Full Text | Google Scholar

Griese, M., Birrer, P., Demirsoy, A. (1997). Pulmonary surfactant in cystic fibrosis. Eur. Respir. J. 10, 1983–1988. doi: 10.1183/09031936.97.10091983

PubMed Abstract | CrossRef Full Text | Google Scholar

Haba, E., Pinazo, A., Jauregui, O., Espuny, M. J., Infante, M. R., Manresa, A. (2003). Physicochemical characterization and antimicrobial properties of rhamnolipids produced by Pseudomonas aeruginosa 47T2 NCBIM 40044. Biotechnol. Bioeng 81, 316–322. doi: 10.1002/bit.10474

PubMed Abstract | CrossRef Full Text | Google Scholar

Han, S., Mallampalli, R. K. (2015). The role of surfactant in lung disease and host defense against pulmonary infections. Ann. Am. Thorac. Soc. 12, 765–774. doi: 10.1513/AnnalsATS.201411-507FR

PubMed Abstract | CrossRef Full Text | Google Scholar

Hoffman, L. R., Déziel, E., D’Argenio, D. A., Lépine, F., Emerson, J., McNamara, S., et al. (2006). Selection for Staphylococcus aureus small-colony variants due to growth in the presence of Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. U.S.A. 103, 19890–19895. doi: 10.1073/pnas.0606756104

PubMed Abstract | CrossRef Full Text | Google Scholar

Hotterbeekx, A., Kumar-Singh, S., Goossens, H., Malhotra-Kumar, S. (2017). In vivo and In vitro Interactions between Pseudomonas aeruginosa and Staphylococcus spp. Front. Cell. Infect. Microbiol. 7. doi: 10.3389/fcimb.2017.00106

PubMed Abstract | CrossRef Full Text | Google Scholar

Howden, B. P., Giulieri, S. G., Wong Fok Lung, T., Baines, S. L., Sharkey, L. K., Lee, J. Y. H., et al. (2023). Staphylococcus aureus host interactions and adaptation. Nat. Rev. Microbiol. 21, 380–395. doi: 10.1038/s41579-023-00852-y

PubMed Abstract | CrossRef Full Text | Google Scholar

Jarvis, F. G., Johnson, M. J. (1949). A glyco-lipide produced by pseudomonas aeruginosa. J. Am. Chem. Soc 71, 4124–4126. doi: 10.1021/ja01180a073

CrossRef Full Text | Google Scholar

Kaito, C., Sekimizu, K. (2007). Colony spreading in Staphylococcus aureus. J. Bacteriol 189, 2553–2557. doi: 10.1128/JB.01635-06

PubMed Abstract | CrossRef Full Text | Google Scholar

Kang, Y., Zarzycki-Siek, J., Walton, C. B., Norris, M. H., Hoang, T. T. (2010). Multiple FadD acyl-CoA synthetases contribute to differential fatty acid degradation and virulence in Pseudomonas aeruginosa. PLoS One 5, e13557. doi: 10.1371/journal.pone.0013557

PubMed Abstract | CrossRef Full Text | Google Scholar

Kasallis, S., Bru, J.-L., Chang, R., Zhuo, Q., Siryaporn, A. (2023). Understanding how bacterial collectives organize on surfaces by tracking surfactant flow. Curr. Opin. Solid State Materials Sci. 27, 101080. doi: 10.1016/j.cossms.2023.101080

CrossRef Full Text | Google Scholar

Kearns, D. B. (2010). A field guide to bacterial swarming motility. Nat. Rev. Microbiol. 8, 634–644. doi: 10.1038/nrmicro2405

PubMed Abstract | CrossRef Full Text | Google Scholar

Kessler, E., Safrin, M., Olson, J. C., Ohman, D. E. (1993). Secreted LasA of Pseudomonas aeruginosa is a staphylolytic protease. J. Biol. Chem. 268, 7503–7508. doi: 10.1016/S0021-9258(18)53203-8

PubMed Abstract | CrossRef Full Text | Google Scholar

Kownatzki, R., Tümmler, B., Döring, G. (1987). Rhamnolipid of Pseudomonas aeruginosa in sputum of cystic fibrosis patients. Lancet 1, 1026–1027. doi: 10.1016/s0140-6736(87)92286-0

PubMed Abstract | CrossRef Full Text | Google Scholar

Kreutzberger, M. A. B., Wang, S., Beltran, L. C., Tuachi, A., Zuo, X., Egelman, E. H., et al. (2022). Phenol-soluble modulins PSMα3 and PSMβ2 form nanotubes that are cross-α amyloids. Proc. Natl. Acad. Sci. U.S.A. 119, e2121586119. doi: 10.1073/pnas.2121586119

PubMed Abstract | CrossRef Full Text | Google Scholar

Limoli, D. H., Warren, E. A., Yarrington, K. D., Donegan, N. P., Cheung, A. L., O’Toole, G. A. (2019). Interspecies interactions induce exploratory motility in Pseudomonas aeruginosa. Elife 8, e47365. doi: 10.7554/eLife.47365

PubMed Abstract | CrossRef Full Text | Google Scholar

Lyczak, J. B., Cannon, C. L., Pier, G. B. (2000). Establishment of Pseudomonas aeruginosa infection: lessons from a versatile opportunist. Microbes Infect. 2, 1051–1060. doi: 10.1016/s1286-4579(00)01259-4

PubMed Abstract | CrossRef Full Text | Google Scholar

McClure, C. D., Schiller, N. L. (1992). Effects of Pseudomonas aeruginosa rhamnolipids on human monocyte-derived macrophages. J. Leukoc. Biol. 51, 97–102. doi: 10.1002/jlb.51.2.97

PubMed Abstract | CrossRef Full Text | Google Scholar

Michelsen, C. F., Christensen, A.-M. J., Bojer, M. S., Høiby, N., Ingmer, H., Jelsbak, L. (2014). Staphylococcus aureus Alters Growth Activity, Autolysis, and Antibiotic Tolerance in a Human Host-Adapted Pseudomonas aeruginosa Lineage. J. Bacteriol 196, 3903–3911. doi: 10.1128/JB.02006-14

PubMed Abstract | CrossRef Full Text | Google Scholar

Morales-Soto, N., Dunham, S. J. B., Baig, N. F., Ellis, J. F., Madukoma, C. S., Bohn, P. W., et al. (2018). Spatially dependent alkyl quinolone signaling responses to antibiotics in Pseudomonas aeruginosa swarms. J. Biol. Chem. 293, 9544–9552. doi: 10.1074/jbc.RA118.002605

PubMed Abstract | CrossRef Full Text | Google Scholar

Morris, J. D., Hewitt, J. L., Wolfe, L. G., Kamatkar, N. G., Chapman, S. M., Diener, J. M., et al. (2011). Imaging and analysis of Pseudomonas aeruginosa swarming and rhamnolipid production. Appl. Environ. Microbiol. 77, 8310–8317. doi: 10.1128/AEM.06644-11

PubMed Abstract | CrossRef Full Text | Google Scholar

Noordman, W. H., Janssen, D. B. (2002). Rhamnolipid stimulates uptake of hydrophobic compounds by Pseudomonas aeruginosa. Appl. Environ. Microbiol. 68, 4502–4508. doi: 10.1128/AEM.68.9.4502-4508.2002

PubMed Abstract | CrossRef Full Text | Google Scholar

Pamp, S. J., Tolker-Nielsen, T. (2007). Multiple roles of biosurfactants in structural biofilm development by Pseudomonas aeruginosa. J. Bacteriol 189, 2531–2539. doi: 10.1128/JB.01515-06

PubMed Abstract | CrossRef Full Text | Google Scholar

Papenfort, K., Bassler, B. L. (2016). Quorum sensing signal-response systems in Gram-negative bacteria. Nat. Rev. Microbiol. 14, 576–588. doi: 10.1038/nrmicro.2016.89

PubMed Abstract | CrossRef Full Text | Google Scholar

Peschel, A., Otto, M. (2013). Phenol-soluble modulins and staphylococcal infection. Nat. Rev. Microbiol. 11, 667–673. doi: 10.1038/nrmicro3110

PubMed Abstract | CrossRef Full Text | Google Scholar

Pollitt, E. J. G., Crusz, S. A., Diggle, S. P. (2015). Staphylococcus aureus forms spreading dendrites that have characteristics of active motility. Sci. Rep. 5, 17698. doi: 10.1038/srep17698

PubMed Abstract | CrossRef Full Text | Google Scholar

Rieger, B., Thierbach, S., Ommer, M., Dienhart, F. S. V., Fetzner, S., Busch, K. B. (2020). Pseudomonas Quinolone Signal molecule PQS behaves like a B Class inhibitor at the IQ site of mitochondrial complex I. FASEB Bioadv 2, 188–202. doi: 10.1096/fba.2019-00084

PubMed Abstract | CrossRef Full Text | Google Scholar

Saadati, F., Shahryari, S., Sani, N. M., Farajzadeh, D., Zahiri, H. S., Vali, H., et al. (2022). Effect of MA01 rhamnolipid on cell viability and expression of quorum-sensing (QS) genes involved in biofilm formation by methicillin-resistant Staphylococcus aureus. Sci. Rep. 12, 14833. doi: 10.1038/s41598-022-19103-w

PubMed Abstract | CrossRef Full Text | Google Scholar

Salsgiver, E. L., Fink, A. K., Knapp, E. A., LiPuma, J. J., Olivier, K. N., Marshall, B. C., et al. (2016). Changing epidemiology of the respiratory bacteriology of patients with cystic fibrosis. Chest 149, 390–400. doi: 10.1378/chest.15-0676

PubMed Abstract | CrossRef Full Text | Google Scholar

Schwartz, K., Syed, A. K., Stephenson, R. E., Rickard, A. H., Boles, B. R. (2012). Functional amyloids composed of phenol soluble modulins stabilize Staphylococcus aureus biofilms. PLoS Pathog. 8, e1002744. doi: 10.1371/journal.ppat.1002744

PubMed Abstract | CrossRef Full Text | Google Scholar

Siegmund, I., Wagner, F. (1991). New method for detecting rhamnolipids excreted byPseudomonas species during growth on mineral agar. Biotechnol. Tech 5, 265–268. doi: 10.1007/BF02438660

CrossRef Full Text | Google Scholar

Soberón-Chávez, G., Lépine, F., Déziel, E. (2005). Production of rhamnolipids by Pseudomonas aeruginosa. Appl. Microbiol. Biotechnol. 68, 718–725. doi: 10.1007/s00253-005-0150-3

PubMed Abstract | CrossRef Full Text | Google Scholar

Tayeb-Fligelman, E., Tabachnikov, O., Moshe, A., Goldshmidt-Tran, O., Sawaya, M. R., Coquelle, N., et al. (2017). The cytotoxic Staphylococcus aureus PSMα3 reveals a cross-α amyloid-like fibril. Science 355, 831–833. doi: 10.1126/science.aaf4901

PubMed Abstract | CrossRef Full Text | Google Scholar

Tsompanidou, E., Denham, E. L., Becher, D., de Jong, A., Buist, G., van Oosten, M., et al. (2013). Distinct roles of phenol-soluble modulins in spreading of Staphylococcus aureus on wet surfaces. Appl. Environ. Microbiol. 79, 886–895. doi: 10.1128/AEM.03157-12

PubMed Abstract | CrossRef Full Text | Google Scholar

Tsompanidou, E., Sibbald, M. J. J. B., Chlebowicz, M. A., Dreisbach, A., Back, J. W., van Dijl, J. M., et al. (2011). Requirement of the agr locus for colony spreading of Staphylococcus aureus. J. Bacteriol 193, 1267–1272. doi: 10.1128/JB.01276-10

PubMed Abstract | CrossRef Full Text | Google Scholar

Wade, D. S., Calfee, M. W., Rocha, E. R., Ling, E. A., Engstrom, E., Coleman, J. P., et al. (2005). Regulation of Pseudomonas quinolone signal synthesis in Pseudomonas aeruginosa. J. Bacteriol 187, 4372–4380. doi: 10.1128/JB.187.13.4372-4380.2005

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, R., Braughton, K. R., Kretschmer, D., Bach, T.-H. L., Queck, S. Y., Li, M., et al. (2007). Identification of novel cytolytic peptides as key virulence determinants for community-associated MRSA. Nat. Med. 13, 1510–1514. doi: 10.1038/nm1656

PubMed Abstract | CrossRef Full Text | Google Scholar

Xavier, J. B., Kim, W., Foster, K. R. (2011). A molecular mechanism that stabilizes cooperative secretions in Pseudomonas aeruginosa: Metabolic prudence in P. aeruginosa. Mol. Microbiol. 79, 166–179. doi: 10.1111/j.1365-2958.2010.07436.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Yang, A., Tang, W. S., Si, T., Tang, J. X. (2017). Influence of physical effects on the swarming motility of pseudomonas aeruginosa. Biophys. J. 112, 1462–1471. doi: 10.1016/j.bpj.2017.02.019

PubMed Abstract | CrossRef Full Text | Google Scholar

Yarlagadda, V., Wright, G. D. (2019). Membrane-active rhamnolipids overcome aminoglycoside resistance. Cell Chem. Biol. 26, 1333–1334. doi: 10.1016/j.chembiol.2019.09.015

PubMed Abstract | CrossRef Full Text | Google Scholar

Yarrington, K. D., Shendruk, T. N., Limoli, D. H. (2022). Twitching cells use a chemoreceptor to detect bacterial competitors. bioRxiv. doi: 10.1101/2022.11.28.518211

CrossRef Full Text | Google Scholar

Ying, L., Zhu, H., Shoji, S., Fredrick, K. (2019). Roles of specific aminoglycoside-ribosome interactions in the inhibition of translation. RNA 25, 247–254. doi: 10.1261/rna.068460.118

PubMed Abstract | CrossRef Full Text | Google Scholar

Zaman, M., Andreasen, M. (2020). Cross-talk between individual phenol-soluble modulins in Staphylococcus aureus biofilm enables rapid and efficient amyloid formation. Elife 9, e59776. doi: 10.7554/eLife.59776

PubMed Abstract | CrossRef Full Text | Google Scholar

Zarrella, T. M., Khare, A. (2022). Systematic identification of molecular mediators of interspecies sensing in a community of two frequently coinfecting bacterial pathogens. PLoS Biol. 20, e3001679. doi: 10.1371/journal.pbio.3001679

PubMed Abstract | CrossRef Full Text | Google Scholar

Zheng, Y., Joo, H.-S., Nair, V., Le, K. Y., Otto, M. (2018). Do amyloid structures formed by Staphylococcus aureus phenol-soluble modulins have a biological function? Int. J. Med. Microbiol. 308, 675–682. doi: 10.1016/j.ijmm.2017.08.010

PubMed Abstract | CrossRef Full Text | Google Scholar

Keywords: surfactant flow, swarm repulsion, tendril organization, biofilms, cystic fibrosis, rhamnolipids, structured illumination, lung surfactant

Citation: Bru J-L, Kasallis SJ, Chang R, Zhuo Q, Nguyen J, Pham P, Warren E, Whiteson K, Høyland-Kroghsbo NM, Limoli DH and Siryaporn A (2023) The great divide: rhamnolipids mediate separation between P. aeruginosa and S. aureus. Front. Cell. Infect. Microbiol. 13:1245874. doi: 10.3389/fcimb.2023.1245874

Received: 23 June 2023; Accepted: 23 August 2023;
Published: 15 September 2023.

Edited by:

Tagbo H. R. Niepa, University of Pittsburgh, United States

Reviewed by:

Martina Valentini, University of Geneva, Switzerland
Nicolas Jacquier, Centre Hospitalier Universitaire Vaudois (CHUV), Switzerland

Copyright © 2023 Bru, Kasallis, Chang, Zhuo, Nguyen, Pham, Warren, Whiteson, Høyland-Kroghsbo, Limoli and Siryaporn. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Albert Siryaporn, asirya@uci.edu

These authors have contributed equally to this work and share first authorship

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