Barbara C. Kahl
Karen Moreau- 1Institute of Medical Microbiology, University Hospital Münster, Münster, Germany
- 2CIRI, Centre International de Recherche en Infectiologie, Université de Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, Lyon, France
Editorial on the Research Topic
Co-Infection and Consequences in Cystic Fibrosis
Cystic fibrosis (CF) is the most common inherited disease in the Caucasian population with app. 70,000 individuals affected worldwide (Elborn, 2016). This disease is linked to a mutation in the cftr gene, resulting in alteration of the CFTR protein, a chloride channel located at the apical site of epithelial cells in many organs (Zielenski et al., 1991). At the pulmonary level, the mutation is responsible for the presence of viscous mucus which inhibits ciliary beat. The modification of the pulmonary environment and the alteration of mucociliary clearance promote the implantation and multiplication of specific pathogens linked to CF and thus cause a modification of the pulmonary microbiota (Hauser et al., 2011; Egan, 2016; Saint-Criq and Gray, 2017; French Cystic Fibrosis Register, 2017; Foundation ACF, 2020; Society ECF, 2020). Chronic bacterial respiratory infections in people with CF (pwCF) are responsible for progressive lung disease with exacerbations and bronchiectasis leading to morbidity and mortality with a reduced life expectancy of 46 years. It is estimated that 80% of deaths are due to bacterial infections of the lungs (Turcios, 2020).
In the CF modified lung environment, mostly specific microbial species (Pseudomonas aeruginosa, Staphylococcus aureus, Stenotrophomonas maltophilia, Burkholderia cepacia complex, Achromobacter xylosoxidans, Exophiala dermatitidis, Aspergillus fumigatus and some viruses) are found and presumably can interact synergistically or antagonistically (Boutin et al., 2015; Bisht et al., 2020; Khanolkar et al., 2020; Eichelberger and Cassat, 2021). These interactions themselves generate changes in the composition of the pulmonary microbiota and may also modify the adaptation and virulence of certain pathogens.
The clinical consequences of polymicrobial infections are of major interest. Understanding the mechanisms of microbial interactions and their clinical relevance is essential to rationally orient current therapies and to develop improved treatments for respiratory infections. Deciphering the impact of co-infection and microbial interactions on the host response at the cellular and molecular levels remains a largely unexplored field. The same is true for the role and impact of polymicrobial infections on the evolution and adaptation of pathogens in the context of chronic infection/colonization.
This Research Topic begins with a very nice review by Biswas and Götz in which they discuss the complexity of bacterial interactions in the context of polymicrobial infection. They describe the competitive and cooperative interactions of the two major CF pathogens, S. aureus and P. aeruginosa, and the molecular mechanisms involved. We are thus discovering that by focusing on only two bacterial species, multiple interactions can be set up.
However, the pulmonary microbiome of cystic fibrosis patients is much more complex and evolves with the age of the patients. Metzger et al. address the question of the link between diversity and dynamic of the microbiome and the decline in respiratory function. By following the composition of the lung microbiome of 12 adolescents for 3 to 5 years, they show that the presence of a diverse and dynamic microbiome is associated with a less severe decline in lung function. Conversely, the presence of a stable, low diversity microbiome dominated by Bacteroidetes and Firmicutes appears to be a major contributor to the severity of the infection. The protective role of a diverse microbiome is reinforced by the study of Tony-Odigie et al. By exploring in vitro, the impact of commensal bacterial species on the inflammatory response induced by P. aeruginosa, they show that several commensal bacteria exhibit a protective effect, including certain strains of Streptococcus mitis. The simultaneous presence of S. mitis and P. aeruginosa reduces the activation of pro-inflammatory signaling pathways in pulmonary epithelial cells. Comparative genomic analysis of S. mitis strains has identified potential genes involved in this protective role. These recent findings pave the way for numerous studies aimed at understanding how the diversity of the lung microbiome influences the host response.
In order to explore this question Graf et al. propose a novel protocol for metaproteomic analysis of CF patient sputum. This protocol allows to analyze the physiology of bacteria as closely as possible to in vivo conditions and may allow to identify the metabolic pathways involved and essential in a polymicrobial context.
In summary, the articles in this Research Topic discuss important features of the complexity of the lung microbiome in cystic fibrosis patients, its evolutionary dynamics and its impact on the evolution of lung function. The field of research concerning these issues is vast and promises many more major scientific studies.
Author Contributions
All authors listed have made a substantial, direct, and intellectual contribution to the work and approved it for publication.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s Note
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Acknowledgments
The editors would like to thank the authors who contributed to this Research Topic, the reviewers who constructively appraised the manuscripts, and Frontiers Editorial Office for their kind support.
References
(2017) French Cystic Fibrosis Register. Available at: https://www.vaincrelamuco.org/sites/default/files/rapport_du_registre_-_donnees_2017.pdf.
Bisht, K., Baishya, J., Wakeman, C. A. (2020). Pseudomonas Aeruginosa Polymicrobial Interactions During Lung Infection. Curr. Opin. Microbiol. 53, 1–8. doi: 10.1016/j.mib.2020.01.014
Boutin, S., Graeber, S. Y., Weitnauer, M., Panitz, J., Stahl, M., Clausznitzer, D., et al. (2015). Comparison of Microbiomes From Different Niches of Upper and Lower Airways in Children and Adolescents With Cystic Fibrosis. PloS One 10, 1–19. doi: 10.1371/journal.pone.0116029
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Foundation ACF. (2020). CFF Annual Patient Data Registry 2019. Bethesda, Maryland: Cystic Fibrosis Foundation.
Hauser, A. R., Jain, M., Bar-Meir, M., McColley, S. A. (2011). Clinical Significance of Microbial Infection and Adaptation in Cystic Fibrosis. Clin. Microbiol. Rev. 24, 29–70. doi: 10.1128/CMR.00036-10
Khanolkar, R. A., Clark, S. T., Wang, P. W., Hwang, D. M., Yau, Y. C. W., Waters, V. J., et al. (2020). Ecological Succession of Polymicrobial Communities in the Cystic Fibrosis Airways. mSystems 5, e00809–e00820. doi: 10.1128/mSystems.00809-20
Saint-Criq, V., Gray, M. A. (2017). Role of CFTR in Epithelial Physiology. Cell Mol. Life Sci. 74, 93–115. doi: 10.1007/s00018-016-2391-y
Society ECF. (2020). Annual Data Report (Year 2018). Brussels, Belgium: European Cyclists’ Federation.
Turcios, N. L. (2020). Cystic Fibrosis Lung Disease: An Overview. Respir. Care 65, 233–251. doi: 10.4187/respcare.06697
Keywords: cystic fibrosis, microbiome, co-infection, bacterial interaction, evolution and adaptation, host response, clinical outcome
Citation: Kahl BC and Moreau K (2022) Editorial: Co-Infection and Consequences in Cystic Fibrosis. Front. Cell. Infect. Microbiol. 12:924527. doi: 10.3389/fcimb.2022.924527
Received: 20 April 2022; Accepted: 05 May 2022;
Published: 01 June 2022.
Edited and reviewed by:
John S. Gunn, The Research Institute at Nationwide Children’s Hospital, United StatesCopyright © 2022 Kahl and Moreau. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Karen Moreau, a2FyZW4ubW9yZWF1QHVuaXYtbHlvbjEuZnI=