Corrigendum: Brain Microvascular Endothelial Cell-Derived HMGB1 Facilitates Monocyte Adhesion and Transmigration to Promote JEV Neuroinvasion

A Corrigendum on:
Brain Microvascular Endothelial Cell-Derived HMGB1 Facilitates Monocyte Adhesion and Transmigration to Promote JEV Neuroinvasion

By Zou S-S, Zou Q-C, Xiong W-J, Cui N-Y, Wang K, Liu H-X, Lou W-J, Higazy D, Zhang Y-G and Cui M (2021). Front. Cell. Infect. Microbiol. 11:701820. doi: 10.3389/fcimb.2021.701820

In the original article, there was a mistake in Figures 2A, B as published. There were spelling errors of the icon of Figures 2A, B. “LAF-1” should be “LFA-1”. The corrected Figure 2 appears below.

FIGURE 2

Figure 2 JEV infection upregulated adhesion molecules expression in bEnd.3 cells, and rHMGB1 increased the expression of integrin ligands in splenocytes. (A) ICAM-1 expression level was detected in the JEV-infected bEnd.3 cells at 6 h. Representative images showing the binding of highly expressed GFP+ LFA-1 yeast cells. (B) Statistical analysis of fluorescence was performed, and the result represents the expression levels of ICAM-1 in bEnd.3 cells. (C) Detection of ICAM-2, VCAM-1, E-selectin (CD62E), VE-cadherin, and beta-catenin expression levels in JEV-infected bEnd.3 cells were determined by Western blotting. Protein samples were collected at 0 h (Control), 6 h, 12 h, and 24 h. (D) The protein expressions reported in panel (C) were normalized to that of beta-actin and quantitatively analyzed as the fold change relative to the control. The expression levels of LFA-1 (CD11a and CD18) (E) and VLA-4 (CD49d and CD29) (F) in rHMGB1-treated (100 ng/ml) mouse splenocytes, determined by real-time PCR at 0 h (Control), 3 h, 6 h, 12 h, 24 h, 48 h. Untreated cells were served as control. The scale bar for (A) is 50 mm. The experiments were repeated at least three times. The data are expressed as the means ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001.

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

In the original article, there was a mistake in Figures 3A, B as published. There were spelling errors of the icon of Figures 3A, B. “Adhsrent” should be “Adherent”. The corrected Figure 3 appears below.

FIGURE 3

Figure 3 HMGB1 promoted immune cell adhesion to the BMEC monolayer. (A) GFP+ splenocytes were incubated with JEV-P3/UV-P3-infected bEnd.3 cell monolayers for 2 h; the GFP+ splenocytes were obtained from transgenic mice. After washing with PBS, the cells were fixed with 4% paraformaldehyde and then observed by fluorescence microscopy. The fluorescence represents the number of adherent splenocytes. JEV free monolayers were served as control. (B) Statistical analysis of the GFP+ splenocytes binding to the bEnd.3 cell monolayer of (A). (C) Mouse splenocytes were treated with rHMGB1 (100 ng/ml) for 2 h. Then, splenocytes (rHMGB1 treated or untreated) were incubated with the bEnd.3 cell monolayers (JEV infected or uninfected) for 2 h. After gentle washing with PBS, the adherent splenocytes were collected, and the amount of CD19+ B cells, CD3+ T cells, and Ly6C+CD11b+ monocytes was analyzed by flow cytometry. Untreated splenocytes and uninfected monolayers were served as control. (D) Statistical analysis of the binding splenocytes to the bEnd.3 cell monolayer as reported in (C). (E) Purified CD3+ T cells and CD11b monocytes [obtained from JEV-infected mice, tail vein injection (F), 3 dpi] were inoculated onto the virus-infected bEnd.3 cell monolayer and incubated for 2 h. After washing with PBS, the bound cells were detected by flow cytometry. The right panels show the results of the statistical analysis of CD3+ T cells and CD11b monocytes binding to the bEnd.3 cell monolayer of (F). JEV free bEnd.3 cell monolayers were served as control. The scale bar for (A) is 100 mm. These experiments were repeated at least three times. The data are expressed as the means ± SEM. p > 0.05 (ns, no significant difference), *p < 0.05, **p < 0.01, and ***p < 0.001.

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

In the original article, there was a mistake in the Supplementary material as published. There was an error of the caption of uncropped Supplementary Figure S1A.“Uncropped Supplementary Figure 1A” should be captioned “Uncropped Figure 1F”. The corrected Supplementary material (Uncropped Figure 1F) appears below.

UNCROPPED FIGURE 1F

Uncropped Figure 1F

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

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Supplementary Material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2021.771928/full#supplementary-material