This article is a correction to:
Profiling the Urinary Microbiota in Male Patients With Bladder Cancer in China
Peng Wu
Guihao Zhang
Jie Zhao2†
Jiawei Chen
Yang Chen
Weina Huang
Jialei Zhong
Jiarong Zeng- 1Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, China
- 2School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, China
A Corrigendum on
Profiling the Urinary Microbiota in Male Patients With Bladder Cancer in China
by Wu, P., Zhang, G., Zhao, J., Chen, J., Chen, Y., Huang, W., et al. (2018). Front. Cell. Infect. Microbiol. 8:167. doi: 10.3389/fcimb.2018.00167
In the original article, there was an error. Readers were unable to access the 16S sequencing data submitted to the Short Read Archive (SRA) under the accession number SUB3915640.
A correction has been made to MATERIALS AND METHODS, DNA Isolation and 16S rRNA Gene Sequencing:
“To avoid contamination, DNA isolation was performed using the cultured cells protocol supplied with the DNeasy Blood and Tissue Kit (Qiagen, Germany) in a laminar flow hood. The concentration of extracted DNA was determined through a Nanodrop ND-1000 spectrophotometer (Thermo Electron Corporation, USA). The genomic DNA isolated from the clinical samples was amplified using primer sets specific for V4 regions (515F: GTGCCAGCMGCCGCGGTAA; and 806R:GGACTACHVGGGTWTCTAAT). In order to evaluate contribution of extraneous DNA from reagents, extraction negative controls (no urine) and PCR negative controls (no template) were included. The resultant PCR products were purified by Qiaquick PCR purification kit (Qiagen, Valencia, CA). Finally, purified samples were normalized to equal DNA concentration and sequenced using the Illumina Miseq sequencer (Illumina, Inc., USA). The 16S rRNA gene sequences have been submitted to the Short Read Archive (SRA) under accession number PRJNA486651”.
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
Keywords: urinary bladder neoplasms, urinary tract, microbiota, extracellular matrix, inflammation
Citation: Wu P, Zhang G, Zhao J, Chen J, Chen Y, Huang W, Zhong J and Zeng J (2018) Corrigendum: Profiling the Urinary Microbiota in Male Patients With Bladder Cancer in China. Front. Cell. Infect. Microbiol. 8:429. doi: 10.3389/fcimb.2018.00429
Received: 29 November 2018; Accepted: 29 November 2018;
Published: 11 December 2018.
Approved by:
Frontiers in Cellular and Infection Microbiology Editorial Office, Frontiers Media SA, SwitzerlandCopyright © 2018 Wu, Zhang, Zhao, Chen, Chen, Huang, Zhong and Zeng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Peng Wu, doctorwupeng@gmail.com
†These authors have contributed equally to this work