Phyto-fabrication of silver nanoparticles and their catalytic dye degradation and antifungal efficacy

The biogenic synthesis of silver nanoparticles (AgNPs) and their potent application against dye degradation and phytopathogens are attracting many scientists to nanotechnology. An attempt was made to synthesize silver nanoparticles using Plantago ovata leaf extract and test their effectiveness in removing organic dyes and antifungal activity. In the present study, stable AgNPs were synthesized from 0.1 mM AgNO₃ and authenticated by observing the color change from yellow to red-brown, which was confirmed with wavelength UV-Vis spectrophotometer detection. The crystalline nature of the particles was characterized by x-ray diffraction (XRD) patterns. Furthermore, the AgNPs were characterized by high-resolution transmission electron microscope and scanning electron microscope investigations. Atomic force microscopy (AFM) and Raman spectra were also used to confirm the size and structure of the synthesized AgNPs. The elemental analysis and functional groups responsible for the reduction of AgNPs were analyzed by electron dispersive spectroscopy and fourier transform infra-red spectroscopy Fourier transforms infrared, respectively. A new biological approach was taken by breaking down organic dyes such as methylene blue and congo red. The AgNPs effectively inhibit the fungal growth of Alternaria alternata. This could be a significant achievement in the fight against many dynamic pathogens and reduce dye contamination from waste water.

Introduction

Nanotechnology has emerged in recent years as a promising and versatile technology that deals with the processing of the separation, consolidation and deformation of materials by an atom or by a molecule (Shah et al., 2020). It is a multidisciplinary field that includes the formation, usage, and manipulation of materials with a scale smaller than 100 nm at one dimension (Nakkala et al., 2018), (Raj et al., 2021). Nanoparticles are attracting the attention of current researchers due to their high surface-area-to-volume ratio, which leads to differences in physical and chemical properties compared to bulk counterparts with the same composition (Raj et al., 2021), (Dashora et al., 2022). It opened the doors for rapidly growing technologies because it was involved in the creation and production of innovative materials with unique and superior material properties (Dhand et al., 2016). Metal nanoparticles (such as Ag, Au, and Cu) have been chosen for their exceptional physicochemical properties, including excellent catalytic activity, optical, magnetic, thermal conductivity, biological, and chemical properties, due to the large specific surface area and a high fraction of surface atoms (Rashid et al., 2019), (Seku et al., 2021). Silver is the metal of choice among the various precious metals due to its many unique properties, most notably its powerful antibacterial and catalytic nature. Various physicochemical techniques such as chemical reduction have been used to synthesize AgNPs (Abdelmoteleb et al., 2020). In recent years, conventional approaches to the synthesis of AgNPs have rarely been used. This is due to the use of hazardous compounds in these processes associated with high energy consumption, corrosive nature and expensive further processing (Khanal et al., 2022). Various approaches have been used for the synthesis of metallic NPs, among which the greener route is considered to be an environmentally benign method (Ali et al., 2022). To formulate the NPs, the biological route has received much more attention compared to the rest of the modern techniques (both physical and chemical) due to several important characteristics such as safety, eco-friendly protocols with non-toxic by-products, requirements for gentle reaction conditions and the use of a natural one capping. Biogenic synthesis includes such as fungi (Tyagi et al., 2019), (Osorio-Echavarria et al., 2021), bacteria (Saeed et al., 2020), algae (Bhuyar et al., 2020) and plants (Raj et al., 2018) for the synthesis of AgNPs and found to be a clean, nontoxic and environmentally acceptable technique (Kaur and Jaryal, 2018), (Varadavenkatesan et al., 2020). Plant part extracts such as root, flower and leaves etc (Sahayaraj et al., 2020; Elangovan et al., 2021; Ilahi et al., 2021) are more effective than microorganism-mediated procedures because plants and plant extracts are less sensitive to metal toxicity, cell culture is not required and metabolites such as polyphenols, saponins, tannins, alkaloids, flavonoids, steroids and others are widely available. These metabolites have the ability to stabilize and reduce the silver ions for the synthesis of NPs (Bharathi and Bhuvaneshwari, 2019), (Mosaviniya et al., 2019).

Toxic organic dyes could be used in a variety of industries, including textiles, leather, plastic, paper, paint, cosmetics and pharmaceuticals (Wang et al., 2018), (Singh et al., 2021). Direct contact of the dye with the organisms can lead to serious health problems such as mental health problems, eye damage and central nervous system. The sequestration of synthetic organic compounds from wastewater is a critical environmental issue (Varadavenkatesan et al., 2019). Many physical and chemical methods such as adsorption, ozonation, photocatalysis, microwave-based degradation, chemo-catalysis, Fenton reaction and electrocatalysis can be used to eliminate these pollutants (Nasrollahzadeh et al., 2018). The complete removal of synthetic dyes from wastewater through physical and chemical processes required high technology that is both inefficient and energy-intensive (Bonigala et al., 2018). The removal of hazardous organic pollutants from wastewater using metallic nanoparticles is a unique strategy and a viable alternative to physical and chemical processes (Eisa et al., 2019). AgNPs have been extensively studied among the numerous metallic NPs because of their relatively low cost (Wang et al., 2019). Therefore, a simple and green biosynthetic technique mediated by Plantago ovata leaf extract was developed in this study.

Plantago ovata is a member of the Plantaginaceae family and is a winter annual plant known locally as Indian Plantago, Desert Indian Wheat, Isabagol, and Ispaghula. It grows in different regions of the world, mainly in the northern hemisphere between the 26th and 36th latitude (Meyers and Liston, 2008). In India, mainly cultivated in Rajasthan and Gujarat as a crop and for medicinal purposes. Plantago leaves are used for various ailments to treat intestinal disorders and bowel habits, as well as constipation, haemorrhoids, skin irritation, diarrhoea, colon cancer, diabetes, excessive cholesterol and inflammatory bowel diseases such as ulcerative colitis (Ahmadi et al., 2012), (Franco et al., 2020). The plant leaves contain many phytochemicals such as alkaloids, caffeic acid derivatives, coumarins, lipids and oils, mucilage, polysaccharides, sterols and salicylic acid, etc. (Nawaz et al., 2021), (Kumar et al., 2020). These active chemicals could be crucial in reducing silver ions to AgNPs. There is no report on the synthesis of silver nanoparticles using Plantago ovata extract and evaluation of their catalytic and antifungal properties, despite the potential of these chemicals in the green synthesis of metal NPs.

Plant pathogenic fungi Fusarium oxysporum and Alternaria alternata cause various diseases in economically important crops. These phytopathogenic fungi can damage pre- or post-harvest crops as their wide host range can cause a variety of damage depending on the susceptibility of the host, the virulence of the isolate, and the infected regions of the plant (Kgatshe et al., 2019). The use of fungicides to combat fungal diseases is the most effective way. These fungicides are mostly synthetic and have numerous health hazards and multiple environmental and human health side effects (Isa and Lockman, 2019). Various natural and safe substitutes for chemical fungicides have been investigated in recent years. In these, biogenic metal NPs were also used to combat phytopathogenic fungi (Khanal et al., 2022). In the food industry, textile composition, and many environmental applications, AgNPs have been widely used in the form of antimicrobial agents (Mekky et al., 2021). However, few records exist on the potential of AgNPs to combat phytopathogenic fungi.

In this present study, we have reported biogenic and eco-friendly synthesis of silver nanoparticles employing Plantago ovata plant extract and characterized by various analytical techniques such as UV-Vis, FT-IR, HR-TEM, FE-SEM, EDS, XRD, AFM and Raman spectra. Further, their catalytic degradation properties against azo dyes MB and CR and in vitro antifungal activity against phytopathogenic fungi Alternaria alternata and Fusarium oxysporum were analysed.

Materials and methods

Materials

All the analytical grade chemicals such as silver nitrate (AgNO₃), sodium borohydride (NaBH₄), methylene blue (MB), congo red (CR), potato dextrose agar (PDA), were procured from Sigma-Aldrich, St. Louis, United States and Himedia Pvt. Ltd. New Delhi, India. Fungal culture of Fusarium oxysporum (ITCC No. 4998) and Alternaria alternata (ITCC No. 6134) were obtained from IARI New Delhi. Plantago ovata plants were collected from Sikar district Rajasthan, prepare herbarium and submitted to the herbarium of Rajasthan University Jaipur, Rajasthan, India to obtain voucher number. The voucher specimen (RUBL211771) was retained in the department for future reference. Figure 1 shows the overview of AgNP synthesis, characterization and applications.

FIGURE 1

FIGURE 1. Schematic illustration of the synthesis of AgNPs and their characterization and applications.

Preparation of the plant extract

Collected plant leaves were washed thoroughly with a tap and followed by double distilled water to remove dust particles and shad dried for 1 week at room temperature. Dried leaves were pulverized with a blender and made into fine powder. Take 5 g of leaf powder mixed with 200 ml sterile deionized water in 500 ml Erlenmeyer flask and boil at 60°C serological water bath for 20 min. After cooling the extract was filtrated through Whatman No. 1 filter paper and stored at 4°C in the refrigerator for further use within a week.

Synthesis of AgNPs with Plantago ovata leaf extract

For AgNPs synthesis 10 ml of plant extract mixed with 90 ml of AgNO₃ solution was taken from 1 mM stock solution of AgNO₃ and incubated at 40°C for 24 h. The whole process was done in low light to avoid photo-oxidation of AgNO₃. The light yellow colour of the solution gradually turn into reddish-brown colour which indicated the reduction of Ag⁺ to Ag⁰. This synthesis of AgNPs was monitored using UV-VIS spectrophotometer scanning between 300 and 700 nm. Afterwards, the solution was centrifuged for 15 min at 10,000 rpm, the supernatant was discarded and pellets were collected. The pellets were washed with de-ionized water and dried in a lyophilizer to make a fine powder.

Characterization of AgNPs

For the initial characterization of synthesized AgNPs, a UV-Visible spectrophotometer (Systronics 117 UV–VIS spectrophotometer) was used. After 24 h of AgNPs synthesis, absorbance was measured between the wavelength ranges of 300–700 nm with a resolution of 1 nm in a quartz cuvette cell with a 1 cm path length. Further characterization of AgNPs was done using Fourier transforms infrared (FTIR) PerkinElmer Spectrum 2 spectrometer at room temperature in the range 500–4000 cm-1 (PerkinElmer, Inc. Waltham, MA, United States). Crystalline metallic AgNPs were examined using an XRD (Rigaku, Ultima IV, Japan). Powder of AgNPs was analyzed by XRD using CuK radiation (1.54 nm) and a scanning 2θ angle ranging from 20^o to 80^o. The polydispersity index (PDI), zeta size and zeta potential of AgNPs were analyzed using DLS (Malvern Instrument Inc., London, United Kingdom) and were taken at a scattering angle of 90^o at a 25°C temperature range. Atomic Force Microscopy (AFM) Multimode Scanning Probe Microscope (Bruker) Germany, was used to analyse surface morphology, and 2D and 3D topography of synthesized AgNPs. The surface morphology of the synthesized AgNPs was carried out using TEM (TEM-Tecnai G2–20, United States) and SEM (JEOL SM-7600F Japan) combined with energy-dispersive X-ray spectroscopy (Oxford EDS system) from Sophisticated Analytical Instrument Facility (SAIF), IIT Bombay. Raman Spectroscopy was used to detect the AgNPs molecular structure and chemical bonds were carried out using lso Plane SCT-320, PIXIS 100 Princeton Instrument from MNIT Materials Research Centre (MRC), Jaipur, Rajasthan, India.

Catalytic degradation of dyes

The degradation of organic dyes MB and CR was carried out by using biosynthesized AgNPs as a catalyst. Before commencement, the AgNPs were dispersed in deionized water using an Ultra probe sonicator for 10 min and a stock solution for dye was prepared. The 3 ml reaction mixture contains desired volume of dyes, NaBH₄ and AgNPs as mentioned in Table 1. The progress of the reaction was monitored by using the UV–vis spectrophotometer (Systronics 117 UV–VIS spectrophotometer). The absorbance of each dye was measured at 1 min time intervals and the change in absorbance at each time interval was examined for kinetic study under different conditions. Reaction mixture with AgNPs and NaBH₄ separately used as control. A reaction mixture without AgNPs was used as a reference and pseudo-first-order kinetics was analyzed to evaluate the rate constant as per the following equation:

$\mathrm{I}\mathrm{n}\left({\mathrm{A}}_{\mathrm{t}}/{\mathrm{A}}_0\right)=-{\mathrm{k}}_{\mathrm{t}}(1)$

TABLE 1

TABLE 1. Detail of experiment for catalytic degradation of dyes.

The % degradation of the dyes was estimated through the following equation

$\mathrm{P}\mathrm{e}\mathrm{r}\mathrm{c}\mathrm{e}\mathrm{n}\mathrm{t}\mathrm{d}\mathrm{e}\mathrm{g}\mathrm{r}\mathrm{a}\mathrm{d}\mathrm{a}\mathrm{t}\mathrm{i}\mathrm{o}\mathrm{n}={\mathrm{A}}_0-{\mathrm{A}}_{\mathrm{t}}/{\mathrm{A}}_0\times 100(2)$

Where, A₀ is the initial absorbance of dye, A_t is the absorbance of dye at time t and k is the rate constant. The whole reaction of degradation was processed at room temperature.

Antifungal activity of synthesized nanoparticles

The antifungal activity of AgNPs against A. alternata and F. oxysporum was carried out using the poison food method (Grover and Moore, 1962). AgNPs at different concentrations (25, 50, 75 and 100 μg/ml) were added to 20 mL of sterilized and cooled autoclaved PDA medium along with synthetic fungicides Bavistin and Mancozeb at 100 μg/ml, plant extract and water as control. After the media solidify the mycelial disc of 6 mm diameter from 7 days old pure culture was placed at the centre of each Petri plate and incubated at 28 ± 2°C. After 7 days of incubation, observations were recorded of fungal mycelium growth inhibition and the percentage of inhibition of fungal growth was calculated as per the following formula given by Vincent (Vincent, 1947).

$\%\mathrm{M}\mathrm{y}\mathrm{c}\mathrm{e}\mathrm{l}\mathrm{i}\mathrm{u}\mathrm{m}\mathrm{i}\mathrm{n}\mathrm{h}\mathrm{i}\mathrm{b}\mathrm{i}\mathrm{t}\mathrm{i}\mathrm{o}\mathrm{n}=\frac{Gc-Gt}{Gc}\times 100(3)$

Where Gc is the diameter of test fungal mycelia growth in a control plate and Gt is the diameter of test fungi mycelia growth in a treated plate.

Spore germination effect

The activity of spore germination inhibition of AgNPs has been assayed against F. oxysporum and A. alternata fungi as described by Saharan et al., 2015 (Saharan et al., 2015). Different concentrations of AgNPs (25, 50, 75 and 100 μg/ml), 100 μg/ml of AgNO₃, Bavistin and Mancozeb, and water as control were used to determine spore germination activity. The spore suspensions were made in sterilized distilled water containing 1×10³ spores/ml. On the glass depression slide (Himedia, Mumbai, India), 50 μl of test fungi suspensions and 50 μl of different concentrations of AgNPs were also added in the same cavity. To maintain the required humidity, each cavity slide was incubated at 28 ± 2°C for 12 h. Three replicates of each treatment, including standard, were maintained and observed under a microscope to calculate spores germination inhibition rate.

$\%\mathrm{s}\mathrm{p}\mathrm{o}\mathrm{r}\mathrm{e}\mathrm{i}\mathrm{n}\mathrm{h}\mathrm{i}\mathrm{b}\mathrm{i}\mathrm{t}\mathrm{i}\mathrm{o}\mathrm{n}=\frac{\mathrm{N}\mathrm{u}\mathrm{m}\mathrm{b}\mathrm{e}\mathrm{r}\mathrm{o}\mathrm{f}\mathrm{s}\mathrm{p}\mathrm{o}\mathrm{r}\mathrm{e}\mathrm{g}\mathrm{e}\mathrm{r}\mathrm{m}\mathrm{i}\mathrm{n}\mathrm{a}\mathrm{t}\mathrm{e}\mathrm{d}}{\mathrm{T}\mathrm{o}\mathrm{t}\mathrm{a}\mathrm{l}\mathrm{n}\mathrm{u}\mathrm{m}\mathrm{b}\mathrm{e}\mathrm{r}\mathrm{o}\mathrm{f}\mathrm{s}\mathrm{p}\mathrm{o}\mathrm{r}\mathrm{e}\mathrm{e}\mathrm{x}\mathrm{a}\mathrm{m}\mathrm{i}\mathrm{n}\mathrm{e}\mathrm{d}}\times 100(4)$

Statistical analysis

Statistical analysis was performed using analysis of variance (ANOVA), followed by a Tukey HSD test (p = 0.05) using SPSS Version 26. The Microsoft Office, OriginPro 2020 and Corel Draw software were used for calculation and designing Graphs and figures.

Results and discussion

UV–visible spectroscopy

The primary investigation of AgNP synthesis was confirmed by UV-Vis absorption spectra scanning between 300 and 700 nm. Deionized water was used as a reference. The peak of the synthesized AgNPs was observed at 414 nm (Figure 2). It was shown that the phytocompounds contained in the leaf extract successfully reduced the Ag + ions to AgNPs since it is known that colloidal silver shows a typical absorption band in the range of 400–450 nm due to the surface plasmon resonance of the conducting electrons of the metal (David and Moldovan, 2020). During the process of AgNPs synthesis, adding a reducing agent, P. ovata aqueous extract in AgNO₃ solution resulted in a colour change from pale yellow to reddish-brown was observed. However, the reduction of silver salt was initiated as the increasing pH of the aqueous extract towards slight alkaline (pH 9). These indicated that the pH plays a crucial role in reducing silver salts to silver ions (Parthiban et al., 2019). It has been revealed by many previous studies that alkaline pH can efficiently reduce the nanoparticles and further encapping them. In their research on the impact of pH on the synthesis of nanoparticles, Sathishkumar et al. (2009) noted the formation of small, stable nanoparticles at an alkaline pH (Sathishkumar et al., 2009). They claim that lower pH values encourage the nucleation of nanoparticles whereas higher pH results in electrostatic repulsion, which causes the creation of smaller nanoparticles. The functional groups in the plant extract have reduced power, which is less active at acidic pH, but it becomes more active as the pH rises to basic pH, resulting in an increase in their reductional power and the development of thermodynamically advantageous structures. Alkaline conditions are therefore favourable for biosynthesis.

FIGURE 2

FIGURE 2. (A) UV-visible spectra of synthesized AgNPs [Inlet (a) AgNO3 (b) Plant Extract (c) AgNPs].

FTIR spectroscopy

FTIR spectroscopy was performed to identify the functional groups associated with the biomolecule involved in AgNP reduction and stability shown in Figure 3. The significant absorption bands at 3,702.37 and 3,332.82 cm⁻¹ correspond to OH and C-H stretches, indicating that alcohol and alkyne are present (Hasnain et al., 2019). Similarly, prominent peaks appeared in the IR spectra at 1,594.97 and 1,384.51 cm⁻¹ ascribed to C=C stretches of cyclic alkenes (aromatic compounds) and C-H stretches of the amide II bond, which confirmed the presence of proteins (Al-Nuairi et al., 2020). Peaks at 1,075.68 cm⁻¹ relate to C-O stretching of the carboxyl ether group and peaks at 984.20 cm⁻¹ are due to C-OH stretching of the carboxyl ether group (Nouri et al., 2020). The C-H stretch of the aromatic group was assigned to the peak at 856.97 cm⁻¹ while the OH bend of the phenol group was assigned to the peak at 540.81 cm⁻¹ (Hawar et al., 2022). These distinct absorption peaks indicated the presence of phytochemicals from extracts (such as amino acids, carbohydrates, phenols, flavonoids, alkaloids, terpenoids, proteins, and water-soluble biomolecules, among others) on the surface of NPs, which were responsible for capping and efficient stabilization of AgNPs and prevention of aggregation of AgNPs (Jemilugba et al., 2019). The morphology (size, shape) and overall functional properties of biosynthesized AgNPs are significantly influenced by the nature of the biomolecules involved in silver ion capping and reduction (Sharma et al., 2020a). According to FTIR analysis, the aromatic compounds (C=C), ether groups (C-O), hydroxyl (-OH) and carboxyl groups (C-OH) in the leaf extract are most likely involved in the reduction.

FIGURE 3

FIGURE 3. FTIR spectra of synthesized AgNPs.

X-ray diffraction

XRD patterns were used to analyze the crystalline nature, structure and size of synthesized AgNPs from Plantago leaf extract. The XRD pattern showed the Bragg reflection plane in the 2θ range of 20–80° and prominent peaks at two values, which can be attributed to the 111, 200, 220, and 311 crystal planes of AgNPs, at 38.22, 46.32, 64.34 and 76.46 shown in Figure 4. These peaks signify that the silver particles with a crystallite size of 17.09 nm have a face-cantered cubic structure and the results are comparable with TEM data (Gulbagca et al., 2019). Other peaks were observed for the 2θ values, which are due to residues of different organic components of the plant extract. These peaks indicated that various plant metabolite units are crystallized on the surface of AgNPs, consistent with the results of Saba Pirtarighat et al. (Pirtarighat et al., 2019). The Braggs diffraction peaks were observed by XRD analysis, which agreed well with a database of the Joint Committee on Powder Diffraction Standard of Ag (JCPDS Card No. 04-0783). There were few unassigned (*) peaks, possibly due to the crystallization of the bioorganic moieties on the surface of the AgNPs, as noted in many previous studies (Raj et al., 2020). From the X-ray magnification, the size of the crystalline NPs was evaluated by the Scherrer equation.

$\mathrm{D}=\mathrm{k}\mathrm{\lambda }/\mathrm{\beta }\cos \mathrm{\theta }(5)$

FIGURE 4

FIGURE 4. XRD pattern of synthesized AgNPs.

Where D is the average crystal size, k is a constant equal to 1, the source of the X-rays is (λ = 1.54), the angular line is full width at half maximum (FWHM) and is the Bragg angle. AgNPs were found to have an average crystal size of 17.09 nm.

DLS analysis

The hydrodynamic size (size and surface water molecule), surface potential, and colloidal stability of biosynthesized AgNPs were studied using the dynamic light scattering (DLS) technique. Before analysis, samples were highly dispersed in water using ultrasound to keep the polydispersity index (PDI) below 0.5, which is much needed during analysis (Mekky et al., 2021). The observed negative potential of biosynthesized AgNPs was 14.9 mV. The negative value could be due to the capping effect of biomolecules present in the leaf extract of P. ovata, which supports the high stability of AgNPs (Figure 5A) (Bharathi and Bhuvaneshwari, 2019). The stability of the synthesized AgNPs was due to the same negative charge on the particles creating a repulsive force between the particles, keeping them suspended in the aqueous solution (Raj et al., 2018). The average size of the AgNPs was 88.3 nm (see supplementary). The polydispersity index (PDI) was estimated to be 0.284, indicating the narrow distribution of the AgNPs (see supplementary). The particle size in DLS appeared larger than in TEM because DLS is measured based on the hydrodynamic diameter (size and surface water molecule) of the particles and gives an intensity-weighted average particle size, while the size obtained from TEM is based on the dry particle diameter and gives an average particle size. (Nakkala et al., 2018). In addition, agglomeration or poor dispersion significantly increases the mean value and thus generally contradicts the SEM/TEM results (Shah et al., 2020).

FIGURE 5

FIGURE 5. DLS analysis (A) Zeta potential, (B) Zeta size of synthesized AgNPs.

AFM analysis

AFM data delineated the biosynthesized AgNPs were roughly spherical with an average grain size of 14.56 nm and root mean square roughness is 10.97 nm. The figure shows the two-dimensional (Figure 6A), three-dimensional (Figure 6C), histogram of sizes obtained from AFM analysis Figures 6B, D shows the roughness profile from the 3D image of synthesized AgNPs calculated. The maximum height of the profile was 37.76 nm, the maximum roughness of valley depth was 13.13 nm and the average maximum height of the roughness was 24.63 nm. These results were consistent with previous studies on the surface morphology of AgNPs in AFM analysis (Bhat et al., 2021), (Yugandhar and Savithramma, 2016). It supports the existence of nanostructures, their fairly homogeneous distribution and the absence of significant agglomeration.

FIGURE 6

FIGURE 6. AFM analysis (A) 2D image (B) Histogram, (C) 3D image, (D) Surface roughness.

TEM and SEM-EDX analysis

TEM images (Figure 7) show fine silver NPs that are roughly spherical, have a smooth surface and are well scattered with a tight, compact arrangement in TEM images, consistent with SEM results. From the TEM figures, it was observed that the particles were predominantly spherical, discrete, polydisperse, and distorted spherical shapes with uniform distribution (AryanRubyMehata, 2021). The calculated value of the biosynthesized AgNPs agreed with the selected area electron diffraction (SAED) pattern and resembled a good agreement with the XRD results (Figure 7E). The SAED image shows distinct and brilliant points in a circular ring, arising from the numerous crystallites of the diffraction planes of the face-cantered cubic crystals (111, 200, 220, 311) (Khan et al., 2020). According to FE-SEM analysis, the particles are consistently spherical and have an organic layer coating their rough surface that serves as a capping agent (Figure 8A) (Bhat et al., 2021). The TEM and FE-SEM analyses were used to determine the particles’ sizes, which are primarily in the range of 10–30 nm with an average size was 12.67 nm. The particle size distribution graph is based on fitting the histogram to a Gaussian model as shown in Figure 7F. In addition, the particle size calculated from the histogram agrees closely with the TEM images.

FIGURE 7

FIGURE 7. TEM analysis (A–D) TEM micrographs of AgNPs at different magnification (E) SAED pattern (F) Histogram shows average particle size distribution of Synthesized AgNPs.

FIGURE 8

FIGURE 8. SEM analysis (A) SEM micrograph (B) EDS spectrum of synthesized AgNPs.

The elemental composition of the reaction mixture was confirmed by EDX. The horizontal axis shows the energy in keV and the vertical axis shows the number of X-ray counts. The EDX result shows (Figure 8B) the presence of silver (75.51%) with a significant peak as well as the additional elements C (7.25%), O (4.25%) and Cl (12, 79%) in weight per cent and Ag (36.04%), C (31.04%), O (14.30%), and Cl (18.58%) in atomic per cent. No ionic silver peak is observed in the EDX spectrum, confirming the bio-reduction of Ag⁺ to stable Ag⁰ in satisfactory yield. Therefore, according to the observed EDX patterns, the biosynthesized AgNPs are highly pure and crystalline due to the complete bio-reduction of silver ions caused by phytochemicals present in the aqueous extract of P. ovata leaves. The C, O and Cl signals are mainly attributed to the biomolecules in the P. ovata leaf extract (Hambardzumyan et al., 2020), (Vijayan et al., 2018). The presence of elemental silver as the main ingredient was verified by EDX analysis, which was possible due to the successful conjugation of P. ovata to AgNPs.

Raman spectra analysis

A Raman spectroscopy study was performed to investigate the detailed interactions between capping components and AgNPs and to examine the functional groups (Bélteky et al., 2019). The significant absorption bands at 2,847 cm⁻¹ correspond to the C-H stretching vibrations and show the hydroxypropyl group shown in Figure 9. Symmetric and asymmetric vibrations of C=O bonds were observed at the peaks 1,330 cm⁻¹ and 1,538 cm⁻¹. This refers to the presence of essential amino acids with aromatic side chains (Abdel-Azeem et al., 2020). The bands at 1,538 cm⁻¹ and 1,330 cm⁻¹ indicate the presence of AgNPs. The other peak obtained at 562 cm⁻¹ could be related to the bending vibrations of N-H associated with the amide structure (Katta and Dubey, 2021). The interaction between the leaf extract and AgNO₃ may be seen in the Raman spectra of produced AgNPs, which have a peak at 335 cm⁻¹. The existence of the silver lattice vibrational models is indicated by the band at 152 cm⁻¹ (Kgatshe et al., 2019).

FIGURE 9

FIGURE 9. Raman spectroscopy of synthesized AgNPs.

Catalytic activity

The degradation of MB was performed by AgNPs synthesized from P. ovata leaf extract. The highest absorption value of pure MB is 664 nm, which corresponds to the MB n→π* transition (Figure 10A). AgNPs and their composites have a larger surface area than other NPs, resulting in higher catalytic activity in dye removal and degradation processes. Norain et al. investigated the reduction of MB by biosynthesized AgNPs (Isa and Lockman, 2019). During the process, slow reduction was seen in the presence of the powerful reducing agent NaBH₄. After the addition of an amount of biosynthesized AgNPs in the dye solution in the presence of NaBH₄, the dye gradually decreases and then shifts towards a higher wavelength. The reduction in absorbance indicates that biosynthesized AgNPs are capable of degrading MB. The reaction is completed in 20 min and MB colour is completely degraded. Which shows a significant decrease in MB absorbance and an increase in the SPR peak of AgNPs at 664 nm shown in Figure 10, Which frequently shows the electron relay effect in which electron transfer occurs between leaf extract mediated AgNPs and MB dye. The linear expression of Lagergren pseudo-first order for MB adsorption by P. ovata mediated AgNPs is shown in Figure 10C. The absorption of biosynthesized AgNPs with a rate constant of 0.056 min⁻¹ is interpreted by our results. Results matched with Catharanthus roseus leaves mediated AgNPs and their efficiency in catalytic and adsorption kinetics investigations of MB dye reported in a previous study (Anjum and Riazunnisa, 2021). For the breakdown of colours, most industrial chemists use NaBH₄, a powerful reducing agent. In the presence of NaBH₄ and AgNPs separately, the reaction proceeded slowly and only a small change in absorbance was observed, as shown in Figures 10A, 11A (Albeladi et al., 2020).

FIGURE 10

FIGURE 10. UV-visible absorption spectra (A) MB with AgNPs and NaBH₄ separately (B) Catalytic degradation of MB by NaBH₄ in the presence of AgNPs, (C) Pseudo-first order plot of ln (A_t/A₀) vs time of MB, (D) Percent degradation of MB with time by AgNPs.

FIGURE 11

FIGURE 11. UV-visible absorption spectra pf (A) CR with AgNPs and NaBH₄ separately (B) Catalytic degradation of CR by NaBH₄ in the presence of AgNPs (C) Pseudo-first order plot of the ln (A_t/A₀) vs. time of CR (D) Percent degradation of CR with time by AgNPs.

The catalytic degradation of carcinogenic CR dye using P. ovata leaf extract mediated AgNPs in the presence of NaBH₄ was investigated in this work. Because of their complex structure and the inclusion of the diazo group, which provides them with physicochemical, thermal and optical stability and because of health concerns, CR is difficult to biodegrade. Aqueous CR electronic spectra revealed two absorption bands at 498 (π→π*) nm and 348 (π→π*) nm (Figure 11) (Varadavenkatesan et al., 2020). During the process after a long period, NaBH₄ alone was unable to adequately reduce the CR dye. The addition of P. ovata mediated AgNPs to the reaction mixture, on the other hand, resulted in CR dye degradation in just 18 min. The azo bonds (-N=N-) in the dye molecule are destroyed during the CR reduction process, resulting in a variety of aromatic amine derivatives. Abolanle et al., 2020 noted a constant decline in the absorption band strength at 498 and 348 nm as time progressed and the colour changed from radish brown to colourless (Tito et al., 2020). The link between ln (A_t/A₀) and response time was discovered to be linear. The rate constant k was determined to be 0.166 min⁻¹ at room temperature and it follows pseudo-first-order kinetics. The biologically synthesized AgNPs have a lot of promise as catalytic agents for purifying organic dye-contaminated wastewater and industrial effluents because of their stability and strong catalytic activity (Seku et al., 2021). Similar findings from other previous studies on the dye degradation activity of synthetic AgNPs are presented in Table 2.

TABLE 2

TABLE 2. Previous studies of Dye degradation activity of synthesized AgNPs.

As a result, the Langmuir–Hinshelwood model suggested that the catalytic degradation of organic dyes was due to a surface reaction between reactant and AgNPs (Figure 12) (Raj et al., 2020). NaBH₄ serves as both an electron donor and a hydrogen supplier in this scenario. AgNPs act as an intermediate to transfer the electrons between BH₄⁻ ion and dye because of their high negative potential (Edison and Sethuraman, 2012), (Edison et al., 2016). After adding NaBH₄ to a solution having dye and AgNPs, the BH₄⁻ ion from NaBH₄ and dye molecules adsorb on the AgNPs surface, resulting in instantaneous electron and hydrogen transport. Diffusion among adsorbed molecules causes desorption of the colourless degraded by-product that might give additional catalytic sites for the breakdown of MB attributable to AgNPs’ wide surface area.

FIGURE 12

FIGURE 12. Schematic representation of reaction of dye degradation (the figure is adapted from our previous studies Raj et al., 2020).

Antifungal assay of silver nanoparticles

This study clearly showed that AgNPs have good antifungal activity against F. oxysporum and A. alternata (Figures 13, 14). However, the reduction in mycelial growth was observed slightly more in A. alternata than in F. oxysporum. Fusarium, Alternaria, AgNPs concentration and their interaction were very significant source of variation in fungal growth on PDA. The concentration of AgNPs was the most important source of variation, according to the relative contribution, whereas for the control treatment of both fungal strains, the interaction was the least important subject. Regardless of the culture medium, all AgNPs concentrations (25, 50, 75, and 100 μg/ml) were capable of suppressing fungal growth shown in Table 3. As the concentration of AgNPs increases, inhibition generally increased. At a concentration of 100 μg/ml, fungal growth was drastically retarded (Dawoud et al., 2021). As a positive control, the commercial fungicide Bavistin (100 μg/ml) showed 100% inhibition of fungal mycelial development as it is a good antifungal agent but have toxic effects on human. Whereas AgNO₃ (100 μg/ml) showed 63.67 ± 2.30 inhibition for F. oxysporum and 60 ± 1.11% for A. alternata and plant extract was found ineffective in inhibiting mycelia growth of both fungal strains. This dimension allows these NPs to readily enter, aggregate, and interact with cell membranes, thereby inactivating the protein activities that contribute to cell death (Nguyen et al., 2020), (Al-Otibi et al., 2021a).

FIGURE 13

FIGURE 13. In vitro antifungal of synthesized AgNPs against Fusarium oxysporum. (A) Control, (B) Plant extract (C) Bavistin, (D) AgNO₃, (E–H) 25, 50, 75, 100 μg/ml AgNPs.

FIGURE 14

FIGURE 14. In vitro antifungal of synthesized AgNPs against Alternaria alternata. (A) Control, (B) Plant extract (C) Bavistin, (D) AgNO₃, (E–H) 25, 50, 75, 100 μg/ml AgNPs.

TABLE 3

TABLE 3. Mycelial growth inhibition activity of synthesized AgNPs.

It has been reported that engineered AgNPs suppress conidial germination and germ tube development, and significantly reduce conidial outgrowth, in a work by Jian et al. (Jian et al., 2021). These results suggest that AgNPs can effectively prevent phytopathogenic fungi from developing asexually. Regarding AgNP activity, research on various bacteria and fungi has shown that AgNP treatment can compromise cell membrane integrity and permeability. In addition, the RNA-Seq data showed that AgNPs according to the KEGG category can inhibit the transcription of genes associated with cellular energy expenditure and metabolism in F. graminearum (Jian et al., 2021). Similar results were recently observed in fungi, suggesting that the disruption of cellular energy expenditure and metabolic pathways is a key component of AgNPs’ antifungal efficacy (Table 4) (Shen et al., 2020).

TABLE 4

TABLE 4. Previous study of mycelial growth inhibition activity of synthesized AgNPs.

Effect of AgNPs on the spore germination

Significant spore germination suppression of F. oxysporum and A. alternata were detected at a varied concentration of AgNPs, as shown in the results (Table 5). The highest quantities of biosynthesized AgNPs resulted in the greatest suppression of spore germination against F. oxysporum and A. alternata (Talie et al., 2020). For control, the germination rate was 100%. However, it reduced sharply with increased concentration of AgNPs. To be specific, 60.57 and 59.78% spore germination of F. oxysporum and A. alternata were inhibited at AgNO₃ and the 25, 50, 75 and 100 μg/ml of biosynthesized AgNPs shown respectively, 56.39, 61.76, 67.35 and 76.30% of spore inhibition of F. oxysporum and 61.51, 65.31, 68.13 and 79.18% of spore inhibition of A. alternata and highest spore inhibition 76.30% (F. oxysporum) and 79.18% (A. alternata) were absorbed in 100 μg/ml concentration of biosynthesized AgNPs. Conidia germination is the key process for pathogen invasion, high inhibition rate at a low concentration of AgNPs against conidia germination could induce effective pathogen quantity and even avoid its infection of plants (Huang et al., 2022).

TABLE 5

TABLE 5. Inhibition activity of AgNPs on spore germination of F. oxysporum and A. alternata

Conclusion

In this study, the highly stable silver nanoparticles were synthesized in an aqueous leaf extract medium by a simple, cheap and environmentally friendly biological strategy. The characterization of AgNPs was analyzed by UV-Vis result and shows that the peak at 414 nm confirmed the synthesis of AgNPs. The components such as aromatic compound (C=C), ether group (C-O), hydroxyl (-OH), and carboxyl (C-OH) and other biomolecules may have reduced and stabilized the silver ions to Ag NPs, confirmed by FTIR and Raman spectra. The crystalline nature of NPs has been demonstrated by XRD and SAED patterns. SEM and TEM analysis reveal spherical NPs with a particle size of 10–26 nm and an average size of biosynthesized AgNPs of 12.67 nm. The biosynthesized AgNPs show excellent catalytic properties in the degradation of MB and CR dyes under optimal experimental conditions. According to these results, the bio-reduction methods and biosynthesized NPs create new opportunities for the development of novel catalysts with efficient catalytic properties, recyclability, and stability. Furthermore, the synthesized AgNPs were found to have high antifungal activity even at low concentrations, especially in the case of A. alternata, since synthesized AgNPs are small and have better fungicidal activity.

Data availability statement

The data analyzed in this study is subject to the following licenses/restrictions: Data are available on the request to authors and journal. Requests to access these datasets should be directed to c2hhbmlyYWoxOTkyQGdtYWlsLmNvbQ==.

Author contributions

RT, Supervision; Project administration. CG and SR, Execute the Experiment; Formal Analysis; Data Curation. SR, Conceptualization; Methodology; Software; Validation; Figure Preparation. SR and CG, Writing- Original Draft Preparation. RT, SR, CG, AD, and SM were involved in the final development of the project and manuscript preparation and approved the final version of manuscript.

Funding

The authors CG and AD are grateful to CSIR Fellowship (09/172(0108)/2019-EMR-I) and (09/172(0092)/2019-EMR-I) respectively, for their financial support.

Acknowledgments

The authors are thankful to IIT SAIF, Bombay and MNIT Jaipur, Rajasthan for SEM-EDS and TEM analysis. The authors are also grateful to N. Laxmi and P. Baroliya for their help in XRD and FTIR analysis

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Supplementary material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fchem.2022.994721/full#supplementary-material

References

Abdel-Azeem, A., Nada, A. A., O’donovan, A., Thakur, V. K., and Elkelish, A. (2020). Mycogenic silver nanoparticles from endophytic Trichoderma atroviride with antimicrobial activity. J. Renew. Mat. 8 (2), 171–185. doi:10.32604/jrm.2020.08960

CrossRef Full Text | Google Scholar

Abdelmoteleb, A., Valdez-Salas, B., Beltran-Partida, E., and Gonzalez-Mendoza, D. (2020). Green synthesis of silver nanoparticles from abronia villosa as an alternative to control of pathogenic microorganisms. J. Renew. Mat. 8 (1), 69–78. doi:10.32604/jrm.2020.08334

CrossRef Full Text | Google Scholar

Ahmadi, R., Kalbasi-Ashtari, A., Oromiehie, A., Yarmand, M.-S., and Jahandideh, F. (2012). Development and characterization of a novel biodegradable edible film obtained from psyllium seed (Plantago ovata Forsk). J. Food Eng. 109 (4), 745–751. doi:10.1016/j.jfoodeng.2011.11.010

CrossRef Full Text | Google Scholar

Al-Nuairi, A. G., Mosa, K. A., Mohammad, M. G., El-Keblawy, A., Soliman, S., and Alawadhi, H. (2020). Biosynthesis, characterization, and evaluation of the cytotoxic effects of biologically synthesized silver nanoparticles from cyperus conglomeratus root extracts on breast cancer cell line MCF-7. Biol. Trace Elem. Res. 194 (2), 560–569. doi:10.1007/s12011-019-01791-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Al-Otibi, F., Al-Ahaidib, R. A., Alharbi, R. I., Al-Otaibi, R. M., and Albasher, G. (2021). Antimicrobial potential of biosynthesized silver nanoparticles by aaronsohnia factorovskyi extract. Molecules 26 (1), 130. doi:10.3390/molecules26010130

PubMed Abstract | CrossRef Full Text | Google Scholar

Al-Otibi, F., Perveen, K., Al-Saif, N. A., Alharbi, R. I., Bokhari, N. A., Albasher, G., et al. (2021). Biosynthesis of silver nanoparticles using Malva parviflora and their antifungal activity. Saudi J. Biol. Sci. 28 (4), 2229–2235. doi:10.1016/j.sjbs.2021.01.012

PubMed Abstract | CrossRef Full Text | Google Scholar

Albeladi, S. S. R., Malik, M. A., and Al-thabaiti, S. A. (2020). Facile biofabrication of silver nanoparticles using Salvia officinalis leaf extract and its catalytic activity towards Congo red dye degradation. J. Mater. Res. Technol. 9 (5), 10031–10044. doi:10.1016/j.jmrt.2020.06.074

CrossRef Full Text | Google Scholar

Ali, S., Sulaiman, S., Khan, A., Khan, M. R., and Khan, R. (2022). Green synthesized silver nanoparticles (AgNPs) from Parrotiopsis jacquemontiana (Decne) Rehder leaf extract and its biological activities. Microsc. Res. Tech. 85 (1), 28–43. doi:10.1002/jemt.23882

PubMed Abstract | CrossRef Full Text | Google Scholar

Anjum, S. M., and Riazunnisa, K. (2021). Catharanthus roseus leaves as a green source for facile synthesis of silver nanoparticles and their efficacy towards catalytic and adsorption kinetics studies of methylene blue dye. Res. J. Chem. Environ. 25 (5).

Google Scholar

Arya, G., Kumari, R. M., Gupta, N., Kumar, A., Chandra, R., and Nimesh, S. (2018). Green synthesis of silver nanoparticles using prosopis juliflora bark extract: Reaction optimization, antimicrobial and catalytic activities. Artif. Cells Nanomed. Biotechnol. 46 (5), 985–993. doi:10.1080/21691401.2017.1354302

PubMed Abstract | CrossRef Full Text | Google Scholar

Aryan, , , Ruby, , , and Mehata, M. S. (2021). Green synthesis of silver nanoparticles using Kalanchoe pinnata leaves (life plant) and their antibacterial and photocatalytic activities. Chem. Phys. Lett. 778, 138760. doi:10.1016/j.cplett.2021.138760

CrossRef Full Text | Google Scholar

Banu, R., Reddy, G. B., and Mangatayaru, K. G. (2020). Catalytic and antibacterial activity of microwave-assisted synthesis of silver nanoparticles using punica granatum peel extract. AIP Conf. Proc. 2269 (1), 030074. doi:10.1063/5.0019608

CrossRef Full Text | Google Scholar

Bélteky, P., Ronavari, A., Igaz, N., Szerencses, B., Toth, I. Y., Pfeiffer, I., et al. (2019). Silver nanoparticles: Aggregation behavior in biorelevant conditions and its impact on biological activity. Int. J. Nanomedicine 14, 667–687. doi:10.2147/ijn.s185965

PubMed Abstract | CrossRef Full Text | Google Scholar

Bharathi, D., and Bhuvaneshwari, V. (2019). Evaluation of the cytotoxic and antioxidant activity of phyto-synthesized silver nanoparticles using Cassia angustifolia flowers. Bionanoscience 9 (1), 155–163. doi:10.1007/s12668-018-0577-5

CrossRef Full Text | Google Scholar

Bhat, M., Chakraborty, B., Kumar, R. S., Almansour, A. I., Arumugam, N., Kotresha, D., et al. (2021). Biogenic synthesis, characterization and antimicrobial activity of Ixora brachypoda (DC) leaf extract mediated silver nanoparticles. J. King Saud Univ. - Sci. 33 (2), 101296. doi:10.1016/j.jksus.2020.101296

CrossRef Full Text | Google Scholar

Bhuyar, P., Rahim, M. H. A., Sundararaju, S., Ramaraj, R., Maniam, G. P., and Govindan, N. (2020). Synthesis of silver nanoparticles using marine macroalgae Padina sp. and its antibacterial activity towards pathogenic bacteria. Beni. Suef. Univ. J. Basic Appl. Sci. 9 (1), 3–15. doi:10.1186/s43088-019-0031-y

CrossRef Full Text | Google Scholar

Bonigala, B., Kasukurthi, B., Konduri, V. V., Mangamuri, U. K., Gorrepati, R., and Poda, S. (2018). Green synthesis of silver and gold nanoparticles using Stemona tuberosa Lour and screening for their catalytic activity in the degradation of toxic chemicals. Environ. Sci. Pollut. Res. 25 (32), 32540–32548. doi:10.1007/s11356-018-3105-9

PubMed Abstract | CrossRef Full Text | Google Scholar

Dashora, A., Rathore, K., Raj, S., and Sharma, K. (2022). Synthesis of silver nanoparticles employing Polyalthia longifolia leaf extract and their in vitro antifungal activity against phytopathogen. Biochem. Biophys. Rep. 31, 101320. doi:10.1016/j.bbrep.2022.101320

PubMed Abstract | CrossRef Full Text | Google Scholar

David, L., and Moldovan, B. (2020). Green synthesis of biogenic silver nanoparticles for efficient catalytic removal of harmful organic dyes. Nanomaterials 10 (2), 202. doi:10.3390/nano10020202

CrossRef Full Text | Google Scholar

Dawoud, T. M., Yassin, M. A., El-Samawaty, A. R. M., and Elgorban, A. M. (2021). Silver nanoparticles synthesized by Nigrospora oryzae showed antifungal activity. Saudi J. Biol. Sci. 28 (3), 1847–1852. doi:10.1016/j.sjbs.2020.12.036

PubMed Abstract | CrossRef Full Text | Google Scholar

Dhand, V., Soumya, L., Bharadwaj, S., Chakra, S., Bhatt, D., and Sreedhar, B. (2016). Green synthesis of silver nanoparticles using Coffea arabica seed extract and its antibacterial activity. Mater. Sci. Eng. C 58, 36–43. doi:10.1016/j.msec.2015.08.018

PubMed Abstract | CrossRef Full Text | Google Scholar

Edison, T. J. I., and Sethuraman, M. G. (2012). Instant green synthesis of silver nanoparticles using Terminalia chebula fruit extract and evaluation of their catalytic activity on reduction of methylene blue. Process Biochem. 47 (9), 1351–1357. doi:10.1016/j.procbio.2012.04.025

CrossRef Full Text | Google Scholar

Edison, T. N. J. I., Atchudan, R., Kamal, C., and Lee, Y. R. (2016). Caulerpa racemosa: A marine green alga for eco-friendly synthesis of silver nanoparticles and its catalytic degradation of methylene blue. Bioprocess Biosyst. Eng. 39 (9), 1401–1408. doi:10.1007/s00449-016-1616-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Eisa, W. H., Zayed, M. F., Anis, B., Abbas, L. M., Ali, S. S. M., and Mostafa, A. M. (2019). Clean production of powdery silver nanoparticles using Zingiber officinale: The structural and catalytic properties. J. Clean. Prod. 241, 118398. doi:10.1016/j.jclepro.2019.118398

CrossRef Full Text | Google Scholar

Elangovan, M., Ramachandran, D., and Rajesh, K. (2021). Green synthesis of silver nanoparticles using flower extract of hemigraphis colorata as reducing agent and its biological activity. Lett. Appl. NanoBioScience 10, 2646–2654. doi:10.33263/LIANBS104.26462654

CrossRef Full Text | Google Scholar

Franco, E. A. N., Sanches-Silva, A., Ribeiro-Santos, R., and de Melo, N. R. (2020). Psyllium (Plantago ovata Forsk): From evidence of health benefits to its food application. Trends Food Sci. Technol. 96, 166–175. doi:10.1016/j.tifs.2019.12.006

CrossRef Full Text | Google Scholar

Grover, R. K., and Moore, J. D. (1962). Toximetric studies of fungicides against BROWN rot organisms, sclerotinia-fructicola and S-Laxa. Phytopathology 52 (9), 876.

Google Scholar

Gulbagca, F., Ozdemir, S., Gulcan, M., and Sen, F. (2019). Synthesis and characterization of Rosa canina-mediated biogenic silver nanoparticles for anti-oxidant, antibacterial, antifungal, and DNA cleavage activities. Heliyon 5 (12), e02980. doi:10.1016/j.heliyon.2019.e02980

PubMed Abstract | CrossRef Full Text | Google Scholar

Hambardzumyan, S., Sahakyan, N., Petrosyan, M., Nasim, M. J., Jacob, C., and Trchounian, A. (2020). Origanum vulgare L. extract-mediated synthesis of silver nanoparticles, their characterization and antibacterial activities. Amb. Express 10 (1), 162–211. doi:10.1186/s13568-020-01100-9

PubMed Abstract | CrossRef Full Text | Google Scholar

Hasnain, M. S., Javed, M. N., Alam, M. S., Rishishwar, P., Rishishwar, S., Ali, S., et al. (2019). Purple heart plant leaves extract-mediated silver nanoparticle synthesis: Optimization by box-behnken design. Mater. Sci. Eng. C 99, 1105–1114. doi:10.1016/j.msec.2019.02.061

PubMed Abstract | CrossRef Full Text | Google Scholar

Hawar, S. N., Al-Shmgani, H. S., Al-Kubaisi, Z. A., Sulaiman, G. M., Dewir, Y. H., and Rikisahedew, J. J. (2022). Green synthesis of silver nanoparticles from alhagi graecorum leaf extract and evaluation of their cytotoxicity and antifungal activity. J. Nanomater. 2022, 1–8. doi:10.1155/2022/1058119

CrossRef Full Text | Google Scholar

Huang, W., Lan, B. I. N., and Yu, H. (2022). Antifungal activity of biosynthesized silver nanoparticles against three maize leaf phytopathogens. Pak. J. Bot. 54 (1), 291–296. doi:10.30848/pjb2022-1(6)

CrossRef Full Text | Google Scholar

Ilahi, I., Khuda, F., Umar Khayam Sahibzada, M., Alghamdi, S., Ullah, R., Zakiullah, , et al. (2021). Synthesis of silver nanoparticles using root extract of Duchesnea indica and assessment of its biological activities. Arabian J. Chem. 14 (5), 103110. doi:10.1016/j.arabjc.2021.103110

CrossRef Full Text | Google Scholar

Isa, N., and Lockman, Z. (2019). Methylene blue dye removal on silver nanoparticles reduced by Kyllinga brevifolia. Environ. Sci. Pollut. Res. 26 (11), 11482–11495. doi:10.1007/s11356-019-04583-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Jemilugba, O. T., Parani, S., Mavumengwana, V., and Oluwafemi, O. S. (2019). Green synthesis of silver nanoparticles using Combretum erythrophyllum leaves and its antibacterial activities. Colloid Interface Sci. Commun. 31, 100191. doi:10.1016/j.colcom.2019.100191

CrossRef Full Text | Google Scholar

Jian, Y., Chen, X., Ahmed, T., Shang, Q., Zhang, S., Ma, Z., et al. (2021). Toxicity and action mechanisms of silver nanoparticles against the mycotoxin-producing fungus Fusarium graminearum. J. Adv. Res. 38, 1–12. doi:10.1016/j.jare.2021.09.006

PubMed Abstract | CrossRef Full Text | Google Scholar

Katta, V. K. M., and Dubey, R. S. (2021). Green synthesis of silver nanoparticles using Tagetes erecta plant and investigation of their structural, optical, chemical and morphological properties. Mater. Today Proc. 45, 794–798. doi:10.1016/j.matpr.2020.02.809

CrossRef Full Text | Google Scholar

Kaur, H., and Jaryal, N. (2018). Utilization of biogenic tea waste silver nanoparticles for the reduction of organic dyes. Mat. Res. Express 5 (5), 055402. doi:10.1088/2053-1591/aabf4b

CrossRef Full Text | Google Scholar

Kgatshe, M., Aremu, O. S., Katata-Seru, L., and Gopane, R. (2019). Characterization and antibacterial activity of biosynthesized silver nanoparticles using the ethanolic extract of Pelargonium sidoides DC. J. Nanomater. 2019, 1–10. doi:10.1155/2019/3501234

CrossRef Full Text | Google Scholar

Khan, Z. U. H., Shah, N. S., Iqbal, J., Khan, A. U., Imran, M., Alshehri, S. M., et al. (2020). Biomedical and photocatalytic applications of biosynthesized silver nanoparticles: Ecotoxicology study of brilliant green dye and its mechanistic degradation pathways. J. Mol. Liq. 319, 114114. doi:10.1016/j.molliq.2020.114114

CrossRef Full Text | Google Scholar

Khanal, L. N., Sharma, K. R., Paudyal, H., Parajuli, K., Dahal, B., Ganga, G. C., et al. (2022). Green synthesis of silver nanoparticles from root extracts of Rubus ellipticus Sm. and comparison of antioxidant and antibacterial activity. J. Nanomater. 2022, 1–11. doi:10.1155/2022/1832587

CrossRef Full Text | Google Scholar

Kucukcobanoglu, Y., Ayisigi, M., Haseki, S., and Aktas, L. Y. (2021). In situ green synthesis of cellulose based silver nanocomposite and its catalytic dye removal potential against methylene blue. J. Clust. Sci. 33 (1–11), 1623–1633. doi:10.1007/s10876-021-02093-6

CrossRef Full Text | Google Scholar

Kumar, D., Singh, H., Raj, S., and Soni, V. (2020). Chlorophyll a fluorescence kinetics of mung bean (Vigna radiata L.) grown under artificial continuous light. Biochem. Biophys. Rep. 24, 100813. doi:10.1016/j.bbrep.2020.100813

PubMed Abstract | CrossRef Full Text | Google Scholar

Mekky, A. E., Farrag, A. A., Hmed, A. A., and Sofy, A. R. (2021). Antibacterial and antifungal activity of green-synthesized silver nanoparticles using spinacia oleracea leaves extract. Egypt. J. Chem. 64 (10), 0–5792. doi:10.21608/ejchem.2021.74432.3673

CrossRef Full Text | Google Scholar

Meyers, S. C., and Liston, A. (2008). The biogeography of plantago ovata forssk.(plantaginaceae). Int. J. Plant Sci. 169 (7), 954–962. doi:10.1086/589699

CrossRef Full Text | Google Scholar

Mosaviniya, M., Kikhavani, T., Tanzifi, M., Yaraki, M. T., Tajbakhsh, P., and Lajevardi, A. (2019). Facile green synthesis of silver nanoparticles using Crocus Haussknechtii Bois bulb extract: Catalytic activity and antibacterial properties. Colloid Interface Sci. Commun. 33, 100211. doi:10.1016/j.colcom.2019.100211

CrossRef Full Text | Google Scholar

Nair, B., Lija, G., Saranya, K., and Mallikarjunaswamy, G. E. (2020). Green synthesis of silver nanoparticles using aqueous extract of leaves of salacia gambleana and its evaluation of antimicrobial activity. World J. Pharm. Pharm. Sci. 9 (9), 2039–2050. doi:10.20959/wjpps20209-17126

CrossRef Full Text | Google Scholar

Nakkala, J. R., Mata, R., Raja, K., Chandra, V. K., and Sadras, S. R. (2018). Green synthesized silver nanoparticles: Catalytic dye degradation, in vitro anticancer activity and in vivo toxicity in rats. Mater. Sci. Eng. C 91, 372–381. doi:10.1016/j.msec.2018.05.048

PubMed Abstract | CrossRef Full Text | Google Scholar

Naseem, K., Zia Ur Rehman, M., Ahmad, A., Dubal, D., and AlGarni, T. S. (2020). Plant extract induced biogenic preparation of silver nanoparticles and their potential as catalyst for degradation of toxic dyes. Coatings 10 (12), 1235. doi:10.3390/coatings10121235

CrossRef Full Text | Google Scholar

Nasrollahzadeh, M., Mehdipour, E., and Maryami, M. (2018). Efficient catalytic reduction of nitroarenes and organic dyes in water by synthesized Ag/diatomite nanocomposite using Alocasia macrorrhiza leaf extract. J. Mat. Sci. Mat. Electron. 29 (19), 17054–17066. doi:10.1007/s10854-018-9802-9

CrossRef Full Text | Google Scholar

Nawaz, M., Wang, X., Saleem, M. H., Khan, M. H. U., Afzal, J., Fiaz, S., et al. (2021). Deciphering plantago ovata forsk leaf extract mediated distinct germination, growth and physio-biochemical improvements under water stress in maize (Zea mays L.) at early growth stage. Agronomy 11 (7), 1404. doi:10.3390/agronomy11071404

CrossRef Full Text | Google Scholar

Nguyen, D. H., Lee, J., Park, K., Ching, Y., Nguyen, X., Phan, V., et al. (2020). Green silver nanoparticles formed by Phyllanthus urinaria, Pouzolzia zeylanica, and Scoparia dulcis leaf extracts and the antifungal activity. Nanomaterials 10 (3), 542. doi:10.3390/nano10030542

PubMed Abstract | CrossRef Full Text | Google Scholar

Nouri, A., Yaraki, M. T., Lajevardi, A., Rezaei, Z., Ghorbanpour, M., and Tanzifi, M. (2020). Ultrasonic-assisted green synthesis of silver nanoparticles using Mentha aquatica leaf extract for enhanced antibacterial properties and catalytic activity. Colloid Interface Sci. Commun. 35, 100252. doi:10.1016/j.colcom.2020.100252

CrossRef Full Text | Google Scholar

Osorio-Echavarria, J., Osorio-Echavarria, J., Ossa-Orozco, C. P., and Gómez-Vanegas, N. A. (2021). Synthesis of silver nanoparticles using white-rot fungus Anamorphous Bjerkandera sp. R1: Influence of silver nitrate concentration and fungus growth time. Sci. Rep. 11 (1), 3842–3914. doi:10.1038/s41598-021-82514-8

PubMed Abstract | CrossRef Full Text | Google Scholar

Parthiban, E., Manivannan, N., Ramanibai, R., and Mathivanan, N. (2019). Green synthesis of silver-nanoparticles from Annona reticulata leaves aqueous extract and its mosquito larvicidal and anti-microbial activity on human pathogens. Biotechnol. Rep. 21, e00297. doi:10.1016/j.btre.2018.e00297

PubMed Abstract | CrossRef Full Text | Google Scholar

Pawar, J. S., and Patil, R. H. (2020). Green synthesis of silver nanoparticles using Eulophia herbacea (Lindl.) tuber extract and evaluation of its biological and catalytic activity. SN Appl. Sci. 2 (1), 52–12. doi:10.1007/s42452-019-1846-9

CrossRef Full Text | Google Scholar

Pirtarighat, S., Ghannadnia, M., and Baghshahi, S. (2019). Green synthesis of silver nanoparticles using the plant extract of Salvia spinosa grown in vitro and their antibacterial activity assessment. J. Nanostructure Chem. 9 (1), 1–9. doi:10.1007/s40097-018-0291-4

CrossRef Full Text | Google Scholar

Purohit, A., Sharma, R., Shiv Ramakrishnan, R., Sharma, S., Kumar, A., Jain, D., et al. (2022). Biogenic synthesis of silver nanoparticles (AgNPs) using aqueous leaf extract of buchanania lanzan spreng and evaluation of their antifungal activity against phytopathogenic fungi. Bioinorg. Chem. Appl. 2022, 1–9. doi:10.1155/2022/6825150

CrossRef Full Text | Google Scholar

Raj, S., Chand Mali, S., and Trivedi, R. (2018). Green synthesis and characterization of silver nanoparticles using Enicostemma axillare (Lam.) leaf extract. Biochem. Biophys. Res. Commun. 503, 2814–2819. doi:10.1016/j.bbrc.2018.08.045

PubMed Abstract | CrossRef Full Text | Google Scholar

Raj, S., Singh, H., Trivedi, R., and Soni, V. (2020). Biogenic synthesis of AgNPs employing Terminalia arjuna leaf extract and its efficacy towards catalytic degradation of organic dyes. Sci. Rep. 10 (1), 9616–9710. doi:10.1038/s41598-020-66851-8

PubMed Abstract | CrossRef Full Text | Google Scholar

Raj, S., Trivedi, R., and Soni, V. (2021). Biogenic synthesis of silver nanoparticles, characterization and their applications—a review. Surfaces 5 (1), 67–90. doi:10.3390/surfaces5010003

CrossRef Full Text | Google Scholar

Rashid, S., Azeem, M., Khan, S. A., Shah, M. M., and Ahmad, R. (2019). Characterization and synergistic antibacterial potential of green synthesized silver nanoparticles using aqueous root extracts of important medicinal plants of Pakistan. Colloids Surfaces B Biointerfaces 179, 317–325. doi:10.1016/j.colsurfb.2019.04.016

PubMed Abstract | CrossRef Full Text | Google Scholar

Rizwana, H., and Alwhibi, M. S. (2021). Biosynthesis of silver nanoparticles using leaves of Mentha pulegium, their characterization, and antifungal properties. Green Process. Synthesis 10 (1), 824–834. doi:10.1515/gps-2021-0079

CrossRef Full Text | Google Scholar

Saeed, S., Iqbal, A., and Ashraf, M. A. (2020). Bacterial-mediated synthesis of silver nanoparticles and their significant effect against pathogens. Environ. Sci. Pollut. Res. 27 (30), 37347–37356. doi:10.1007/s11356-020-07610-0

PubMed Abstract | CrossRef Full Text | Google Scholar

Saharan, V., Sharma, G., Yadav, M., Choudhary, M. K., Sharma, S., Pal, A., et al. (2015). Synthesis and in vitro antifungal efficacy of Cu–chitosan nanoparticles against pathogenic fungi of tomato. Int. J. Biol. Macromol. 75, 346–353. doi:10.1016/j.ijbiomac.2015.01.027

PubMed Abstract | CrossRef Full Text | Google Scholar

Sahayaraj, K., Balasubramanyam, G., and Chavali, M. (2020). Green synthesis of silver nanoparticles using dry leaf aqueous extract of Pongamia glabra Vent (Fab.), Characterization and phytofungicidal activity. Environ. Nanotechnol. Monit. Manag. 14, 100349. doi:10.1016/j.enmm.2020.100349

CrossRef Full Text | Google Scholar

Sathishkumar, M., Sneha, K., Won, S. W., Cho, C.-W., Kim, S., and Yun, Y.-S. (2009). Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its bactericidal activity. Colloids Surfaces B Biointerfaces 73 (2), 332–338. doi:10.1016/j.colsurfb.2009.06.005

PubMed Abstract | CrossRef Full Text | Google Scholar

Seku, K., Hussaini, S. S., Pejjai, B., Al Balushi, M. M. S., Dasari, R., Golla, N., et al. (2021). A rapid microwave-assisted synthesis of silver nanoparticles using Ziziphus jujuba Mill fruit extract and their catalytic and antimicrobial properties. Chem. Pap. 75 (4), 1341–1354. doi:10.1007/s11696-020-01386-w

CrossRef Full Text | Google Scholar

Shah, M., Nawaz, S., Jan, H., Uddin, N., Ali, A., Anjum, S., et al. (2020). Synthesis of bio-mediated silver nanoparticles from Silybum marianum and their biological and clinical activities. Mater. Sci. Eng. C 112, 110889. doi:10.1016/j.msec.2020.110889

PubMed Abstract | CrossRef Full Text | Google Scholar

Sharma, B., Singh, I., Bajar, S., Gupta, S., Gautam, H., and Kumar, P. (2020). Biogenic silver nanoparticles: Evaluation of their biological and catalytic potential. Indian J. Microbiol. 60 (4), 468–474. doi:10.1007/s12088-020-00889-0

PubMed Abstract | CrossRef Full Text | Google Scholar

Sharma, K., Guleria, S., and Razdan, V. K. (2020). Green synthesis of silver nanoparticles using ocimum gratissimum leaf extract: Characterization, antimicrobial activity and toxicity analysis. J. Plant Biochem. Biotechnol. 29 (2), 213–224. doi:10.1007/s13562-019-00522-2

CrossRef Full Text | Google Scholar

Shen, T., Wang, Q., Li, C., Zhou, B., Li, Y., and Liu, Y. (2020). Transcriptome sequencing analysis reveals silver nanoparticles antifungal molecular mechanism of the soil fungi Fusarium solani species complex. J. Hazard. Mat. 388, 122063. doi:10.1016/j.jhazmat.2020.122063

PubMed Abstract | CrossRef Full Text | Google Scholar

Sherin, L., Sohail, A., Mustafa, M., Jabeen, R., and Ul-Hamid, A. (2020). Facile green synthesis of silver nanoparticles using Terminalia bellerica kernel extract for catalytic reduction of anthropogenic water pollutants. Colloid Interface Sci. Commun. 37, 100276. doi:10.1016/j.colcom.2020.100276

CrossRef Full Text | Google Scholar

Singh, H., Raj, S., Kumar, D., Sharma, S., Bhatt, U., kalaji, H. M., et al. (2021). Tolerance and decolorization potential of duckweed (Lemna gibba) to C.I. Basic Green 4. Sci. Rep. 11 (1), 10889. doi:10.1038/s41598-021-90369-2

PubMed Abstract | CrossRef Full Text | Google Scholar

Talie, M. D., Wani, A. H., Ahmad, N., Bhat, M. Y., and War, J. M. (2020). Green synthesis of silver nanoparticles (AgNPs) using Helvella leucopus Pers. and their antimycotic activity against fungi causing fungal rot of Apple. Asian J. Pharm. Clin. Res. 13 (4), 161–165. doi:10.22159/ajpcr.2020.v13i4.37024

CrossRef Full Text | Google Scholar

Tito, G. S., Abolanle, A. S., Kuvarega, A. T., Idris, A. O., Mamba, B. B., and Feleni, U. (2020). Nickel selenide quantum dot applications in electrocatalysis and sensors. Electroanalysis 32 (12), 2603–2614. doi:10.1002/elan.202060341

CrossRef Full Text | Google Scholar

Tyagi, S., Tyagi, P. K., Gola, D., Chauhan, N., and Bharti, R. K. (2019). Extracellular synthesis of silver nanoparticles using entomopathogenic fungus: Characterization and antibacterial potential. SN Appl. Sci. 1 (12), 1545–1549. doi:10.1007/s42452-019-1593-y

CrossRef Full Text | Google Scholar

Varadavenkatesan, T., Selvaraj, R., and Vinayagam, R. (2019). Dye degradation and antibacterial activity of green synthesized silver nanoparticles using Ipomoea digitata Linn. flower extract. Int. J. Environ. Sci. Technol. (Tehran). 16 (5), 2395–2404. doi:10.1007/s13762-018-1850-4

CrossRef Full Text | Google Scholar

Varadavenkatesan, T., Selvaraj, R., and Vinayagam, R. (2020). Green synthesis of silver nanoparticles using Thunbergia grandiflora flower extract and its catalytic action in reduction of Congo red dye. Mater. Today Proc. 23, 39–42. doi:10.1016/j.matpr.2019.05.441

CrossRef Full Text | Google Scholar

Vijayan, R., Joseph, S., and Mathew, B. (2018). Green synthesis of silver nanoparticles using Nervalia zeylanica leaf extract and evaluation of their antioxidant, catalytic, and antimicrobial potentials. Part. Sci. Technol. 37 (7), 1–11. doi:10.1080/02726351.2018.1450312

CrossRef Full Text | Google Scholar

Vincent, J. M. (1947). Distortion of fungal hyphæ in the presence of certain inhibitors. Nature 159 (4051), 850. doi:10.1038/159850b0

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, F., Zhang, W., Tan, X., Wang, Z., Li, Y., and Li, W. (2019). Extract of Ginkgo biloba leaves mediated biosynthesis of catalytically active and recyclable silver nanoparticles. Colloids Surfaces A Physicochem. Eng. Aspects 563, 31–36. doi:10.1016/j.colsurfa.2018.11.054

CrossRef Full Text | Google Scholar

Wang, L., Lu, F., Liu, Y., Wu, Y., and Wu, Z. (2018). Photocatalytic degradation of organic dyes and antimicrobial activity of silver nanoparticles fast synthesized by flavonoids fraction of Psidium guajava L. leaves. J. Mol. Liq. 263, 187–192. doi:10.1016/j.molliq.2018.04.151

CrossRef Full Text | Google Scholar

Yugandhar, P., and Savithramma, N. (2016). Biosynthesis, characterization and antimicrobial studies of green synthesized silver nanoparticles from fruit extract of Syzygium alternifolium (Wt.) Walp. an endemic, endangered medicinal tree taxon. Appl. Nanosci. 6 (2), 223–233. doi:10.1007/s13204-015-0428-4

CrossRef Full Text | Google Scholar